sp|Q9LZ98|AB20I_ARATH ----> ABC transporter I family member 20

Intron 1 GTTTTCCTAA ... TTTGATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.42	31	0	17
A2	23.81	16	0	5
A3	33.33	28	0	14
A4	13.51	32	0	5
A5	40.91	13	0	9
A6	25.66	84	0	29
A7	23.44	98	0	30
A8	21.31	96	0	26
A9	24.78	85	0	28
A10	27.52	79	0	30

>TRINITY_DN111589_c0_g1_i2 from sample A1 / Example spliced isoform

TACGCGCTCACGAGCTACGTAATCATTTAAACGAATGACCGACCTTCACTCACATTGAGTGCGTGTCTAAAGAGAGACCTCACGGACTAGTAAAACGTTTAAAATAAACATTTGATTGATTACGCGCTCACAAGCTTCGTAATCAAGAAAATGAACGCCCTTTACTCACCTAATCGGCAACCCGAGTGACATGAAATGGA
ATAACATGGATCCAGATCTTGATACTGATGCCACCAGTAGCACCTCCAGAAGAAGCAGCAAAAACTACGATGATGGATAATATGAAAGGTGTGACAGTGGAAATCAACAATCTCAATTTCACTTATCCTGGAATAGATGGCCACCCCCCGCCGGGAGCCAAGCCGCTCATTCAAAACTTCTCGCTCACTCTCAGAGCCGG
GGATCGTTGCCTCCTGGTGGGCGCCAACGGAGCAGGGAAAACCACGATTCTGAAAATACTGGGAGGGAAACACATGGTCGAGCCCCACATGGTCCGCGTGCTCGGGAGATCGGCTTTTCACGATACTTCTTTGACTTCATCCGGCGATCTTTGTTACTTGGGAGGCGAGTGGAGAAGAGATGTCGCATTTGCTGGTTTTG
ATGTTCCGCTGCAAATGGATGTTTCAGCTGAAAAAATGATATTTGGTGTTCACGGAATTGAACCTCAACGAAGAGACCGCCTTATAAAGGTATTAGGCGTTGACCTAAGATGGCGCATGCATAATGTATCAGATGGTCAAAGGAGACGTGTGCAGATTTGTATGGGCCTTCTACAACCATTTAAGGTTCTTCTTCTAGAT
GAGATTACTGTTGACCTAGATGTACTTGCAAGGGCTGATTTATTGTCTTTTTTAAAGCATGAATGTAAAGACAGAGGAGCCACAATTATCTATGCCACACATATCTTTGATGGCCTTGATAATTGGCCATCACACATAGTAAGATATTTTTGGTATACTCTTTACAGCTTTTTAAAATCCATCTGTTTAGAAAAC

>TRINITY_DN111589_c0_g1_i1 from sample A1 / Example unspliced isoform:

GCCTTCTACAACCATTTAAGgttttcctaatcttctttcaaagtcttagtgctttttgatgctataatctataaattttgaccctaaagaccaaaggcaatggacaatatccataagcatagatctgattgtttactttgattgtggtgtgttcatgctcaagaaatatacatagcatttttttaaattgtcttgtgacc
agtttgtattgttttgatgtagGTTCTTCTTCTAGATGAGATTACTGTTGACCTAGATGTACTTGCAAGGGCTGATTTATTGTCTTTTTTTAAAGCATGAATGTAAAGACAGAGGAGCCACAATTATCTATGCCACACATATCTTTGATGGCCTTGATAATTGGCCATCACACATAGTAAGATATTTTTGGTATACTCTT
TACAGCTTTTTAAAATCCATCTGTTTAGAAAAC

Intron 2 GTTCTTCTTC ... TTTTTTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	13	31	34
A2	77.14	4	16	11
A3	87.69	4	28	29
A4	67.09	13	32	21
A5	92	1	13	10
A6	71.56	32	84	77
A7	76.38	30	98	96
A8	70.71	41	96	102
A9	74.42	33	85	107
A10	76.41	23	79	70

>TRINITY_DN36798_c0_g1_i2 from sample A6 / Example spliced isoform

GGAACCATAACCTAAAACTGTGGTTTCTTTGAAAAGAAAACGCTAATCACACAGTGGGTTTATGCTACTCACCGCTTTGCAATCCATAGTGCGTGTCCAAACACAGACCTTACGCGCTCACGAGCTACGTAATCATTTAAACGAATGACCGACCTTCACTCACATTGAGTGCGTGTCTAAAGAGAGACCTCACGGACTAG
TAAAACGTTTAAAATAAACATTTGATTGATTACGCGCTCACAAGCTTCGTAATCAAGAAAATGAACGCCCTTTACTCACCTAATCGGCAACCCGAGTGACATGAAATGGAATAACATGGATCCAGATCTTGATACTGATGCCACCAGTAGCACCTCCAGAAGAAGCAGCAAAAACTACGATGATGGATAATATGAAAGGT
GTGACAGTGGAAATCAACAATCTCAATTTCACTTATCCTGGAATAGATGGCCACCCCCCGCCGGGAGCCAAGCCGCTCATTCAAAACTTCTCGCTCACTCTCAGAGCCGGGGATCGTTGCCTCCTGGTGGGCGCCAACGGAGCAGGGAAAACCACGATTCTGAAAATACTGGGAGGGAAACACATGGTCGAGCCCCACAT
GGTCCGCGTGCTCGGGAGATCGGCTTTTCACGATACTTCTTTGACTTCATCCGGCGATCTTTGTTACTTGGGAGGCGAGTGGAGAAGAGATGTCGCATTTGCTGGTTTTGATGTTCCGCTGCAAATGGATGTTTCAGCTGAAAAAATGATATTTGGTGTTCACGGAATTGAACCTCAACGAAGAGACCGCCTTATAAAGG
TATTAGGCGTTGACCTAAGATGGCGCATGCATAATGTATCAGATGGTCAAAGGAGACGTGTGCAGATTTGTATGGGCCTTCTACAACCATTTAAGCATGAATGTAAAGACAGAGGAGCCACAATTATCTATGCCACACATATCTTTGATGGCCTTGATAATTGGCCATCACACATAGCATATGTTGCTCGTGGCAAACTG
CAGTTAGCATTGCCATTAGAGGAGGTTGAAGAAATGAAAGAACTCTCCCTAATGAGAACAGTAGAGAAGTGGTTGAGGAAAGAGAAGGAAGATGAGAAGAGAATTAGAAAGGCGAGAAGGGCACAGGGTCTCCCAGAATTTGAAGAATATGTTGAAGGTAGCCGTGTTGTTGGAGATCCAGCCAGGGTTGCTGCTCAGCC
ATTGAATAATGGTTGGGCAGGAGGTAGACTGCATTCAACTTTGGCTGGCACAGATCCTTTTGATTTCAGTTCAAATAGTGTCTTAAGATTATAATAATGCTTCAAATCAAATTTGTGAATAATAAAAGCTTCCAAATCAGCATCGAAGCAAGCAGTAACTACTCTTTCTGGGTTATTTGAAAGTGATCCGTAAGGATTAT
TACATGGATTACATGACCCAGGGTATTGGAGTTTGCAATATTCCCAAAATGAAAATTCAATTCTTCAAGGAATCGATAAATATTCAATAAAAATAAATTTATTATTTGTCATTATTGAAGATTGTAAGTTCAACTACTTGGCCTTTGAAATCACTTCTATACTTCCACTTGATATATGTCATGCTTTTGAACTTAACTAG
AAACTTGCCTACCATAGAAATTAAAAATATCCATGTA

>TRINITY_DN36798_c0_g1_i1 from sample A6 / Example unspliced isoform:

GGAACCATAACCTAAAACTGTGGTTTCTTTGAAAAGAAAACGCTAATCACACAGTGGGTTTATGCTACTCACCGCTTTGCAATCCATAGTGCGTGTCCAAACACAGACCTTACGCGCTCACGAGCTACGTAATCATTTAAACGAATGACCGACCTTCACTCACATTGAGTGCGTGTCTAAAGAGAGACCTCACGGACTAG
TAAAACGTTTAAAATAAACATTTGATTGATTACGCGCTCACAAGCTTCGTAATCAAGAAAATGAACGCCCTTTACTCACCTAATCGGCAACCCGAGTGACATGAAATGGAATAACATGGATCCAGATCTTGATACTGATGCCACCAGTAGCACCTCCAGAAGAAGCAGCAAAAACTACGATGATGGATAATATGAAAGGT
GTGACAGTGGAAATCAACAATCTCAATTTCACTTATCCTGGAATAGATGGCCACCCCCCGCCGGGAGCCAAGCCGCTCATTCAAAACTTCTCGCTCACTCTCAGAGCCGGGGATCGTTGCCTCCTGGTGGGCGCCAACGGAGCAGGGAAAACCACGATTCTGAAAATACTGGGAGGGAAACACATGGTCGAGCCCCACAT
GGTCCGCGTGCTCGGGAGATCGGCTTTTCACGATACTTCTTTGACTTCATCCGGCGATCTTTGTTACTTGGGAGGCGAGTGGAGAAGAGATGTCGCATTTGCTGGTTTTGATGTTCCGCTGCAAATGGATGTTTCAGCTGAAAAAATGATATTTGGTGTTCACGGAATTGAACCTCAACGAAGAGACCGCCTTATAAAGG
TATTAGGCGTTGACCTAAGATGGCGCATGCATAATGTATCAGATGGTCAAAGGAGACGTGTGCAGATTTGTATGGGCCTTCTACAACCATTTAAGgttcttcttctagatgagattactgttgacctagatgtacttgcaagggctgatttattgtcttttttaaagCATGAATGTAAAGACAGAGGAGCCACAATTATC
TATGCCACACATATCTTTGATGGCCTTGATAATTGGCCATCACACATAGCATATGTTGCTCGTGGCAAACTGCAGTTAGCATTGCCATTAGAGGAGGTTGAAGAAATGAAAGAACTCTCCCTAATGAGAACAGTAGAGAAGTGGTTGAGGAAAGAGAAGGAAGATGAGAAGAGAATTAGAAAGGCGAGAAGGGCACAGGG
TCTCCCAGAATTTGAAGAATATGTTGAAGGTAGCCGTGTTGTTGGAGATCCAGCCAGGGTTGCTGCTCAGCCATTGAATAATGGTTGGGCAGGAGGTAGACTGCATTCAACTTTGGCTGGCACAGATCCTTTTGATTTCAGTTCAAATAGTGTCTTAAGATTATAATAATGCTTCAAATCAAATTTGTGAATAATAAAAG
CTTCCAAATCAGCATCGAAGCAAGCAGTAACTACTCTTTCTGGGTTATTTGAAAGTGATCCGTAAGGATTATTACATGGATTACATGACCCAGGGTATTGGAGTTTGCAATATTCCCAAAATGAAAATTCAATTCTTCAAGGAATCGATAAATATTCAATAAAAATAAATTTATTATTTGTCATTATTGAAGATTGTAAG
TTCAACTACTTGGCCTTTGAAATCACTTCTATACTTCCACTTGATATATGTCATGCTTTTGAACTTAACTAGAAACTTGCCTACCATAGAAATTAAAAATATCCATGTA

Intron 3 GTAAGATATT ... TATTATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.29	21	9	0
A2	32.5	6.3	3	1
A3	42.05	8.3	6	2
A4	25.4	9.3	3	0
A5	0	3.7	0	0
A6	13.92	62.7	10	0
A7	21.03	76.7	20	0
A8	14.81	101.3	17	0
A9	19.74	79.7	19	0
A10	17.11	53.7	11	0

>TRINITY_DN105957_c0_g1_i1 from sample A3 / Example spliced isoform

TCACATTGAGTGCGTGTCTAAAGAGAGACCTCACGGACTAGTAAAACGTTTAAAATAAACATTTGATTGATTACGCGCTCACAAGCTTCGTAATCAAGAAAATGAACGCCCTTTACTCACCTAATCGGCAACCCGAGTGACATGAAATGGAATAACATGGATCCAGATCTTGATACTGATGCCACCAGTAGCACCTCCAG
AAGAAGCAGCAAAAACTACGATGATGGATAATATGAAAGGTGTGACAGTGGAAATCAACAATCTCAATTTCACTTATCCTGGAATAGATGGCCACCCCCCGCCGGGAGCCAAGCCGCTCATTCAAAACTTCTCGCTCACTCTCAGAGCCGGGGATCGTTGCCTCCTGGTGGGCGCCAACGGAGCAGGGAAAACCACGATT
CTGAAAATACTGGGAGGGAAACACATGGTCGAGCCCCACATGGTCCGCGTGCTCGGGAGATCGGCTTTTCACGATACTTCTTTGACTTCATCCGGCGATCTTTGTTACTTGGGAGGCGAGTGGAGAAGAGATGTCGCATTTGCTGGTTTTGATGTTCCGCTGCAAATGGATGTTTCAGCTGAAAAAATGATATTTGGTGT
TCACGGAATTGAACCTCAACGAAGAGACCGCCTTATAAAGGTATTAGGCGTTGACCTAAGATGGCGCATGCATAATGTATCAGATGGTCAAAGGAGACGTGTGCAGATTTGTATGGGCCTTCTACAACCATTTAAGGTTCTTCTTCTAGATGAGATTACTGTTGACCTAGATGTACTTGCAAGGGCTGATTTATTGTCTT
TTTTAAAGCATGAATGTAAAGACAGAGGAGCCACAATTATCTATGCCACACATATCTTTGATGGCCTTGATAATTGGCCATCACACATAGCATATGTTGCTCGTGGCAAACTGCAGTTAGCATTGCCATTAGAGGAGGTTGAAGAAATGAAAGAACTCTCCCTAATGGTAAGTTGTTAATCATACAACTATGAGTTTACT
TCTGATTTAAATTGATTGAAAACTATTCATATTTGTATGCTACTGCTCAATGTAACTACATGTTGAAAGAATAGTCTTAGCCAATAACATATCTGTAACTAACTGAACATTATGCCTGAGCCAATAACATAACTGTGCACCTGTTCTGTGCTGTTCTGTAAGATTGTGTGCAACTTTTAGACCTGTTAAAGGTATGTACC
TCGGATTGTGCGCTTCTTGCACCCAAATTTGGTCTGCTACATGTTTGTTTGAGACCTTTGTATAGCTGTTACAAGTTTAC

>TRINITY_DN105957_c0_g1_i5 from sample A3 / Example unspliced isoform:

AGGTTCTTCTTCTAGATGAGATTACTGTTGACCTAGATGTACTTGCAAGGGCTGATTTATTGTCTTTTTTAAAGCATGAATGTAAAGACAGAGGAGCCACAATTATCTATGCCACACATATCTTTGATGGCCTTGATAATTGGCCATCACACATAgtaagatatttttggtatactctttacagctttttaaaatccatc
tgtttagaaaactctttttgatattttttctattatacagGCATATGTTGCTCGTGGCAAACTGCAGTTAGCATTGCCATTAGAGGAGGTTGAAGAAATGAAAGAACTCTCCCTAATG