NA ----> NA

Intron 1 GTAAGTTTCT ... AAATTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	7.77	83	8	17.4
A2	6.42	63	4.6	6.4
A3	4.35	64	3	14.2
A4	6.04	45	3.2	9.6
A5	10.28	26	3.6	4.2
A6	3.72	224	9.2	48
A7	3.94	320.2	14	63
A8	3.71	350	14.2	86.8
A9	4.68	293	15.2	72.8
A10	5.14	190	11.2	30

>TRINITY_DN39309_c0_g1_i5 from sample A8 / Example spliced isoform

ATTATCTCCCTCTTCCAAGGCGCCCCCACCCTCTCGCTTTGTCATGTAAAAAGAGATTCGCTGGACGAATTCGAATTTCCCTTCTCACTAACGGCCATTGTTATCGAAGAAAGGGGAAACCTTGAATTACGGGACCGGAGAAATAACGTCGCCGGTCACATGGGCTACGGTTCGATGCGACGAGGGGAGGGCCCAAGAGA
TGGATGACGCTTTTCCCGATGAATCCTCGGCCGTTCCTGTAAATTAAAACAGCACAATAAACCAAATTATAACATACCTTTTAAGAGATGGAAGGAATGGCCTCTGTGTACGTGCTTGTATATATCCGAGGTGCGGGATTGAGCAGAACTCTTCCGAGGGACTGCAATGGCAGTGGAAGTCGAAATGTGGTGGGGATTGC
CAGCGGGTTTAATCTGTTTGGCGGCCATGCTTCTTCTTCTGCTTCTGCTTCTTTTGGCGAAGAAGAACCATCCCACGCCCAAGGATTTGACGCTTTCCACATCTGGTGAAATGGGGAAGCCGAGTAGTAATCAGCAGCGGAGGCTGGTTCTGGAGATCCTCATGTCCTCGGGACGAACCGAGAGCTTCCGGCTAAAGCTG
GAGGCTGACGCCGCCAATTTCACGGCGCTCACAAATCTGGCTTCGTCGCTCTCCTCTGCTGCAAAGGGGTGCCCATGTGCCCATTCGGGCGGCGAAGAAGAAGAGGAGGAGGAAGAAGCTGGGGTTCCGGGCGTGCCGGGGGCGCTTCAAAAGGCCGATTCTGTTTCCAGCCATGTGACCTTTAGCGGTACAGAAAACAA
CGTCCCGGACGCCGAGGCGGAGGAGGGGAATTACGGTGACCTGGAGGCGGTGGCCAAGATGTGCAGCGATGACGTGGCGGTGCTACAGGGAGCGGGATGCGAATCTGATTGGGCAGACGGTAAAGCGGAGAAGAAAAGAAAGCGGCGGCGGAGGAGGAGGAAGAAAAAAGCGGTGGGGAACGGGGAGTTCGTGATGGAGA
GGGGCGGAGAAGAAGAAGAGGGCGAGTTAGGGTGCACATGCGGCAAGTGGAAGGGTGGGGATTGCCACGAGGGGAGCTGCCCCTATCCATTCACCTCCTCAGGCAGAGGCAGCGTGATACAGAGGAGAATTAAGCAGCACTACGATGAGCTCCTCCAATCTAATATGGCCGGTCCTCTCACAATAGCCCAGGTTGGGCGC
TTTACCAACTGCCTTGTGGAGGCAAAAGCTGCACTGCTGCAAAAGTCTGAAACCATTCAACGCAAGTTTACAATAGCAAAGTCACTTTTAGTAAAGGCAGATAAATCATCATTTGATCGCCTTTGTGGGCAGGTTAGTAGTAATGCACTTTTGCAATGAGCAATGTTTGTCCATCTGTTTTACTCTAATTTTACAATTAC
TGGTTATGCTATTCTCTTCACCATCATTTTGGAATCTGGAATTGCTTTTCATGTTATGAGATATTTGAAGTCTACATGATCTATTTTAAGTTTGGTTAATGTTTAGTCCAATACTCTGGTAAGTTTCCCTTCTACTCAATGATTATGGAGTGATACAGATATATAGGCTAGAGACTGAACAAAAGAGATTAGAGGAGGAC
ACCGAAGTTTACAATCGGCTTCAAGAACAGCTCAAGGTTTCTCCAGCCTATCAGAAGATGCTCGAGTATGGTCACAGCCACTTCCAGTCACAGCTCAATACAGGCCAGTTGTTAGAAAAGTCCGAGACAGAAGATGCTGAAATATCTTTTGAGGAGTTATTAGCTCAGGAAAAGAAGGATTCTTTCTGGCAAAAGCATGG
CCATTTCAAGTCATCAATGCCTGTAAGTTTTTGATGCTTTTGGCTTGAATTGCACATGGCAAAGGGAGAGACCGAAGTATCTTTCTTGTATAGTTACAAAGAAGAATCAAATACCTTTGGGGAATTCACGATGTGATGTATGGAATTATTTTTCTTCTTCTTTCACTGATGAATGTGATGCCTGAAACTTCCATTCATGA
TAATATTGTCGAAGTCAAATGTGCAGCTTGTAGACAAGAATATAAGAATATTTCAGTCTACTATAAAATCTCAGGTGTCAATAGAGTGATTCTCTTTAATGATAGCATGTTCCTCGTCTCCAAGCTCCTAGTGCATGTTGGTTTCTTTTGTGCCAAGGAGTATAGTGACCTGTAAACTGCCGTGTATATTCTACTGGAAC
AGTTTGTCACAACAGATCTTGTAATATTCTGAAAATCCCTTATTATGCGG

>TRINITY_DN39309_c0_g1_i3 from sample A8 / Example unspliced isoform:

AGGAAAAGAAGGATTCTTTCTGgtaagtttctgaaccgacattagagaatattttgctgcaggtttcaaaggttttttaatttgtttttaatacaccactaagttactaactcaaatttgcagGCAAAAGCATGGCCATTTCAAGTCATCAATGCCTGTAAGTTTTTGATGCTTTTGGCTTGAATTGCACATGGCAAAGG
GAGAGACCGAAGTATCTTTCTTGTATAGTTACAAAGAAGAATCAAATACCTTTGGGGAATTCACGATGTGATGTATGGAATTATTTTTCTTCTTCTTTCACTGATGAATGTGATGCCTGAAACTTCCATTCATGATAATATTGTCGAAGTCAAATGTGCAGCTTGTAGACAAGAATATAAGAATATTTCAGTCTACTATA
AAATCTCAGGTGTCAATAGAGTGATTCTCTTTAATGATAGCATGTTCCTCGTCTCCAAGCTCCTAGTGCATGTTGGTTTCTTTTGTGCCAAGGAGTATAGTGACCTGTAAACTGCCGTGTATATTCTACTGGAACAGTTTGTCACAACAGATCTTGTAATATTCTGAAAATCCCTTATTATGCGG

Intron 2 GTTAGTAGTA ... AGTGATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.58	99	14.3	10.8
A2	26.45	89	32	9
A3	11.27	63	8	14.3
A4	25	75	25	8.8
A5	22.92	37	11	5.8
A6	17.9	188	41	24
A7	17.34	270.5	56.8	24.5
A8	16.19	233	45	28
A9	17.44	284	60	32.3
A10	15.89	254	48	35.5

>TRINITY_DN113042_c0_g2_i1 from sample A1 / Example spliced isoform

TTATCGAAGAAAGGGGAAACCTTGAATTACGGGACCGGAGAAATAACGTCGCCGGTCACATGGGCAACGGTTCGATGCGACGAGGGGAGGGCCCAAGAGATGGATGACGCTTTTCCCGATGAATCCTCGGCCGTTCCTGTAAATTAAAACAGCACAATAAACCAAATTATAACATACCTTTTAAGAGATGGAAGGAATGG
CCTCTGTGTACGTGCTTGTATATATCCGAGGTGCGGGATTGAGCAGAACTCTTCCGAGGGACTGCAATGGCAGTGGAAGTCGAAATGTGGTGGGGATTGCCAGCGGGTTTAATCTGTTTGGCGGCCATGCTTCTTCTTCTTTTGGCGAAGAAGAACCATCCCACGCCCAAGGATTTGACGCTTTCCACATCTGGTGAAAT
GGGGAAGCCGAGTAGTAATCAGCAGCGGAGGCTGATTCTGGAGATCCTCATGTCCTCGGGACGAACCGAGAGCTTCCGGCTAAAGCTGGAGGCTGACGCCGCCAATTTCACGGCGCTCACAAATCTGGCTTCGTCGCTCTCCTCTGCTGCAAAGGGGTGCCCATGTGCCCATTCGGGCGGCGAAGAAGAAGAGGAGGAGG
AAGAAGCTGGGGTTCCGGGCGTGCCGGGGGCGCTTCAAAAGGCCGATTCTGTTTCCAGCCATGTGACCTTTAGCGGTACAGAAAACAACGTCCCGGACGCCGAGGCGGAGGAGGGGAATTACGGTGACCTGGAGGCGGTGGCCAAGATGTGCAGCGATGACGTGGCGGTGCTACAGGGAGCGGGATGCGAATCTGATTGG
GCAGACGGTAAAGCGGAGAAGAAAAGAAAGCGGCGGCGGAGGAGGAGGAAGAAAAAAGCGGTGGGGAACGGGGAGTTCGTGATGGAGAGGGGCGGAGAAGAAGAAGAGGGCGAGTTAGGGTGCACATGCGGCAAGTGGAAGGGTGGGGATTGCCACGAGGGGAGCTGCCCCTATCCATTCACCTCCTCAGGCAGAGGCAG
CGTGATACAGAGGAGAATTAAGCAGCACTACGATGAGCTCCTCCAATCTAATATGGCCGGTCCTCTCACAATAGCCCAGGTTGGGCGCTTTACCAACTGCCTTGTGGAGGCAAAAGCTGCACTGCTGCAAAAGTCTGAAACCATTCAACGCAAGTTTACAATAGCAAAGTCACTTTTAGTAAAGGCAGATAAATCATCAT
TTGATCGCCTTTGTGGGCAGATATATAGGCTAGAGACTGAACAAAAGAGATTAGAGGAGGACACCGAAGTTTACAATCGGCTTCAAGAACAGCTCAAGGTTTCTCCAGCCTATCAGAAGATGCTCGAGTATGGTCACAGCCACTTCCAGTCACAGCTCAATACAGGCCAGTTGTTAGAAAAGTCCGAGACAGAAGATGCT
GAAATATCTTTTGAGGAGTTATTAGCTCAGGAAAAGAAGGATTCTTTCTGGCAAAAGCATGGCCATTTCAAGTCATCAATGCCTGTAAGTTTTTGATGCTTTTGGCTTGAATTGCACATGGCAAAGGGAGAGACCGAAGTATCTTTCTTGTATAGTTACAAAGAAGAATCAAATACCTTTGGGGAATTCACGATGTGATG
TATGGAATTATTTTTCTTCTTCTTTCACTGATGAATGTGATGCCTGAAACTTCCATTCATGATAATATTGTCGAAGTCAAATGTGCAGCTTGTAGACAAGAATATAAGAATATTTCAGTCTACTATAAAATCTCAGGTGTCAATAGAGTGATTCTCTTTAATGATAGCATGTTCCTCGTCTCCAAGCTCCTAGTGCATGT
TGGTTTCTTTTGTGCCAAGGAGTATAGTGATCTGTAAACTGCCGTGTATATTCTACTGGAACAGTTTGTCACAACAGATCTTGTAATATTCTGAAAATCCCTTATTATGCGGTCTAGTACTTTAGATCTTCAGATTTATTTTTACTAGTTTAATGCATGAACAATGAATAACCTGAAATGAAA

>TRINITY_DN113042_c0_g2_i3 from sample A1 / Example unspliced isoform:

TTATCGAAGAAAGGGGAAACCTTGAATTACGGGACCGGAGAAATAACGTCGCCGGTCACATGGGCAACGGTTCGATGCGACGAGGGGAGGGCCCAAGAGATGGATGACGCTTTTCCCGATGAATCCTCGGCCGTTCCTGTAAATTAAAACAGCACAATAAACCAAATTATAACATACCTTTTAAGAGATGGAAGGAATGG
CCTCTGTGTACGTGCTTGTATATATCCGAGGTGCGGGATTGAGCAGAACTCTTCCGAGGGACTGCAATGGCAGTGGAAGTCGAAATGTGGTGGGGATTGCCAGCGGGTTTAATCTGTTTGGCGGCCATGCTTCTTCTTCTGCTTCTGCTTCTTTTGGCGAAGAAGAACCATCCCACGCCCAAGGATTTGACGCTTTCCAC
ATCTGGTGAAATGGGGAAGCCGAGTAGTAATCAGCAGCGGAGGCTGATTCTGGAGATCCTCATGTCCTCGGGACGAACCGAGAGCTTCCGGCTAAAGCTGGAGGCTGACGCCGCCAATTTCACGGCGCTCACAAATCTGGCTTCGTCGCTCTCCTCTGCTGCAAAGGGGTGCCCATGTGCCCATTCGGGCGGCGAAGAAG
AAGAGGAGGAGGAAGAAGCTGGGGTTCCGGGCGTGCCGGGGGCGCTTCAAAAGGCCGATTCTGTTTCCAGCCATGTGACCTTTAGCGGTACAGAAAACAACGTCCCGGACGCCGAGGCGGAGGAGGGGAATTACGGTGACCTGGAGGCGGTGGCCAAGATGTGCAGCGATGACGTGGCGGTGCTACAGGGAGCGGGATGC
GAATCTGATTGGGCAGACGGTAAAGCGGAGAAGAAAAGAAAGCGGCGGCGGAGGAGGAGGAAGAAAAAAGCGGTGGGGAACGGGGAGTTCGTGATGGAGAGGGGCGGAGAAGAAGAAGAGGGCGAGTTAGGGTGCACATGCGGCAAGTGGAAGGGTGGGGATTGCCACGAGGGGAGCTGCCCCTATCCATTCACCTCCTC
AGGCAGAGGCAGCGTGATACAGAGGAGAATTAAGCAGCACTACGATGAGCTCCTCCAATCTAATATGGCCGGTCCTCTCACAATAGCCCAGGTTGGGCGCTTTACCAACTGCCTTGTGGAGGCAAAAGCTGCACTGCTGCAAAAGTCTGAAACCATTCAACGCAAGTTTACAATAGCAAAGTCACTTTTAGTAAAGGCAG
ATAAATCATCATTTGATCGCCTTTGTGGGCAGgttagtagtaatgcacttttgcaatgagcaatgtttgtccatctgttttactctaattttacaattactggttatgctattctcttcaccatcattttggaatctggaattgcttttcatgttatgagatatttgaagtctacatgatctattttaagtttggttaat
gtttactccaatactctggtaagtttcccttctactcaatgattatggagtgatacagATATATAGGCTAGAGACTGAACAAAAGAGATTAGAGGAGGACACCGAAGTTTACAATCGGCTTCAAGAACAGCTCAAGGTTTCTCCAGCCTATCAGAAGGTAAATACTTTACAGATGGATCAGCAAGCCATTAGGCCAAACT
TTCTAATCCACCCAGCAAACTTCAGCAGTGAGATTGATGTAAATCTACTCTGAATTTGCAAAAACTGCTTTATGTCTG

Intron 3 GTAAATACTT ... CTACCTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.1	104.3	3.7	20
A2	15.55	70.6	3.6	13
A3	7.64	72.5	4.8	6
A4	10.33	60.7	2.7	7
A5	24.57	39.9	0.5	13
A6	13.09	219.1	4.2	33
A7	16.97	279	1.6	57
A8	14.93	285	2.7	50
A9	10.69	275.7	5.9	33
A10	7.34	227.5	7.5	18

>TRINITY_DN112467_c1_g3_i1 from sample A3 / Example spliced isoform

CGGCCATTGTTATCGAAGAAAGGGGAAACCTTGAATTACGGGACCGGAGAAATAACGTCGCCGGTCACATGGGCAACGGTTCGATGCGACGAGGGGAGGGCCCAAGAGATGGATGACGCTTTTCCCGATGAATCCTCGGCCGTTCCTGTAAATTAAAACAGCACAATAAACCAAATTATAACATACCTTTTAAGAGATGG
AAGGAATGGCCTCTGTGTACGTGCTTGTATATATCCGAGGTGCGGGATTGAGCAGAACTCTTCCGAGGGACTGCAATGGCAGTGGAAGTCGAAATGTGGTGGGGATTGCCAGCGGGTTTAATCTGTTTGGCGGCCATGCTTCTTCTTCTTTTGGCGAAGAAGAACCATCCCACGCCCAAGGATTTGACGCTTTCCACATC
TGGTGAAATGGGGAAGCCGAGTAGTAATCAGCAGCGGAGGCTGATTCTGGAGATCCTCATGTCCTCGGGACGAACCGAGAGCTTCCGGCTAAAGCTGGAGGCTGACGCCGCCAATTTCACGGCGCTCACAAATCTGGCTTCGTCGCTCTCCTCTGCTGCAAAGGGGTGCCCATGTGCCCATTCGGGCGGCGAAGAAGAAG
AGGAGGAGGAAGAAGCTGGGGTTCCGGGCGTGCCGGGGGCGCTTCAAAAGGCCGATTCTGTTTCCAGCCATGTGACCTTTAGCGGTACAGAAAACAACGTCCCGGACGCCGAGGCGGAGGAGGGGAATTACGGTGACCTGGAGGCGGTGGCCAAGATGTGCAGCGATGACGTGGCGGTGCTACAGGGAGCGGGATGCGAA
TCTGATTGGGCAGACGGTAAAGCGGAGAAGAAAAGAAAGCGGCGGCGGAGGAGGAGGAAGAAAAAAGCGGTGGGGAACGGGGAGTTCGTGATGGAGAGGGGCGGAGAAGAAGAAGAGGGCGAGTTAGGGTGCACATGCGGCAAGTGGAAGGGTGGGGATTGCCACGAGGGGAGCTGCCCCTATCCATTCACCTCCTCAGG
CAGAGGCAGCGTGATACAGAGGAAAATTAAGCAGCACTACGATGAGCTCCTCCAATCTAATATGGCCGGTCCTCTCACAATAGCCCAGGTTGGGCGCTTTACCAACTGCCTTGTGGAGGCAAAAGCTGCACTGCTGCAAAAGTCTGAAACCATTCAACGCAAGTTTACAATAGCAAAGTCACTTTTAGTAAAGGCAGATA
AATCATCATTTGATCGCCTTTGTGGGCAGATATATAGGCTAGAGACTGAACAAACGAGATTAGAGGAGGACACCGAAGTTTACAATCGGCTTCAAGAACAGCTCAAGGTTTCTCCAGCCTATCAGAAGATGCTCGAGTATGGTCACAGCCACTTCCAGTCACAGCTCAATACAGGCCAGTTGTTAGAAAAGTCCGAGACA
GAAGATGCTGAAATATCTTTTGAGGAGTTATTAGCTCAGGAAAAGAAGGATTCTTTCTGGCAAAAGCATGGCCATTTCAAGTCATCAATGCCTGTAAGTTTTTGATGCTTTTGGCTTGAATTGCACATGGCAAAGGGAGAGACCGAAGTATCTTTCTTGTATAGTTACAAAGAAGAATCAAATACCTTTGGGGAATTCAC
GATGTGATGTATGGAATTATTTTTTTCTTCTTTCACTGATGAATGTGATGCCTGAAACTTCCATTCATGATAATATTGTCGAAGTCAAATGTGCAGCTTGTAGACAAGAATATAAGAATATTTCAGTCTACTATAAAATCTCAGGTGTCAATAGAGTGATTCTCTTTAATGATAGCATGTTCCTCGTCTCCAAGCTCCTA
GTGCATGTTGGTTTCTTTTGTGCCAAGGAGTATAGTGATCTGTAAACTGCCGTGTATATTCTACTGGAACAGTTTGTCACAACAGATCTTGTAATATTCTGAAAATCCCTTATTATGCG

>TRINITY_DN112467_c1_g3_i5 from sample A3 / Example unspliced isoform:

TTTGTCCATCTGTTTTACTCAAATTTTACAATTACTGGTTATGCTATTCTCTTCACCATCATTTTGGAATCTGGAATTGCTTTTCATGTTATGAGATATTTGAAGTCTACATGATCTATTTTAAGTTTGGTTAATGTTTAGTCCAATACTCTGGTAAGTTTCCCTTCTACTCAATGATTATGGAGTGATACAGATATATA
GGCTAGAGACTGAACAAACGAGATTAGAGGAGGACACCGAAGTTTACAATCGGCTTCAAGAACAGCTCAAGGTTTCTCCAGCCTATCAGAAGgtaaatactttacagttggatcagcaagccattaggccaaactttctaatccacccagcaaacttcagcagtgagattgatgtaaatctactctgaatttgcaaaaac
tgctttatgtctgtctgactgggggaagttcacatggtagataaaaatgtctataatgtcataccaaaggagatacattcctttctcagtacaactaaatatttacctctagtttttagcttatggatattcaatttgatgcatgtgtccatgtcttattccattgatcctacctgaagATGCTCGAGTATGGTCACAGC
CACTTCCAGTCACAGCTCAATACAGGCCAGTTGTTAGAAAAGTCCGAGACAGAAGATGCTGAAATATCTTTTGAGGAGTTATTAGCTCAGGAAAAGAAGGATTCTTTCTGGCAAAAGCATGGCCATTTCAAGTCATCAATGCCTGTAAGTTTTTGATGCTTTTGGCTTGAATTGCACATGGCAAAGGGAGAGACCGAAGT
ATCTTTCTTGTATAGTTACAAAGAAGAATCAAATACCTTTGGGGAATTCACGATGTGATGTATGGAATTATTTTTTTCTTCTTTCACTGATGAATGTGATGCCTGAAACTTCCATTCATGATAATATTGTCGAAGTCAAATGTGCAGCTTGTAGACAAGAATATAAGAATATTTCAGTCTACTATAAAATCTCAGGTGTC
AATAGAGTGATTCTCTTTAATGATAGCATGTTCCTCGTCTCCAAGCTCCTAGTGCATGTTGGTTTCTTTTGTGCCAAGGAGTATAGTGATCTGTAAACTGCCGTGTATATTCTACTGGAACAGTTTGTCACAACAGATCTTGTAATATTCTGAAAATCCCTTATTATGCG