sp|O23310|NFYB3_ARATH ----> Nuclear transcription factor Y subunit B-3

Intron 1 GTACTAGTTT ... TTTTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.43	3219	878	682.3
A2	22.39	5526	1594.3	1046.3
A3	23.22	5951	1799.3	1018.7
A4	23.13	4790.7	1442	829.3
A5	25.89	6555.7	2290	976.7
A6	21.95	697	196	155.7
A7	22.4	181.3	52.3	48
A8	20.54	205	53	50.7
A9	24.55	292	95	74
A10	26.62	91	33	13

>TRINITY_DN114555_c0_g1_i1 from sample A2 / Example spliced isoform

CTTTGTTAATTAGATTTCTGAATTTCAACAGTTTTCTGTAGGTGAGAATTGCTATAATTCTGTCTGTTCTTTCGATTTCTCAATTTCAATAGCCGATGGGAAGGGCTCCGAATCATTTTTTTTCTTGCTGTACTAAATTTTGAAAATCAAAGCTTCCTGGCTATTACGAAACTCGAACAGATAGAAAGTGTGGCCGTTGG
AATGAGGCTTGAGTAAAGTCAGCATGACCCTTGAAGGAGCAGCTTGTTCCCTCAGCTCCTGTTCCCTGTATTTCTCTTAACGCCGACGCAGAGGAGTAATGTAGTCCGTGTACCCAACAACTTCTATTGCCACACACCAAATCATTGACACCAATAGTCTTACGGTTCTCTGAGACGCACTTCTCATGTGCCATTCCGGT
GATAAAACTGATAAACTCTGTGACGCACTGCTGCATCAACCCCTTGGTTTCTGTCGACACCTTCGCATTCTTGCCCAACACTTTCTTTCATTATTCTGCTCACGATGGCCGACGGCATATACAACTCATTGGGGTTGTTGCTGTAATTACCACCACTGTTGGTGTTATTGTTGTGTATTTCTTTAAATACCGAAGCCGAG
GAGCTATGCAACACTGCTATTCTTGTTAGATTTCTGCGGAATGAGGCATTGCTATTCTGTCTGCGGAATACCGAAGCCGAGGAATGAAGGCTGCGGAGTGAGGGACTGCTATTCTGATGAGCATTGCTATTCTGTCTGTTTGTTCGATTTCTCAATTTCAATGGCCGATGGGAACAGCTCCGAAACAACTGAAAACGGCA
ACATCAACACTGTCGGTGAGTTAGATTTCTCAAATTTCAATGGCTGATGAGAGCGGTTTCAAATGAACTGATCAACACAACAATAACACCAACACTGGTGGTGATTACAGAAACAACACCAATGAGCTGTTTATGCCGTCGGCCAACGTGACCAGAATAATGAGGAAAGTGTTGAGCGAGGATGCGAAGTTGTCGACAGA
AGCCGAGGAGGTTGATGCAGCAGTGCGTGACAGAGTTTATGAGTTTTGTCACCGGGAAGGCGCACGAGAAGTGCGCCACAGAGAGCCGTAAGACTCTTGGGGTCGATGATTTGTTTTGGGGCAGTAGAAGTTGTTGGGTACACGGACTGCATCATTCCTCTGCGTCGGTATTTATAGAAATACAGGGAACGGGAGCTGAG
CAAACAAGCTGCTGCTTCAGAGGGTCATGGTGGCTTTACTCAACCTCATTCCAACGGCCATACTTTTTATCTGTTCGAGTTTCTCAAAATTTTTGTACAGCAGGAAGAAAATGATAGGTGATTTACTGTCTTGTTGATTGCATCTCAAAACAATCAAGGGCATTCAATTTTTTGTGAGGGCATTCTATTTTTTAATGAGC
TGAAATGGAATTGGGATGCCTTATTTTAGGGCATTCAATTATTTTTAACGACATTCAACTTTTTAAAGATTTTTCAGAAATGCTCAGATCCAACTTTTACCAATGTTCAAGAACAGATTCTTTAATTTTTAATTCAATTACATAGCTGAGTTCTTTCATGTCCTTGCTACTTTTTCACAC

>TRINITY_DN114555_c0_g1_i6 from sample A2 / Example unspliced isoform:

CTTTGTTAATTAGATTTCTGAATTTCAACAGTTTTCTGTAGGTGAGAATTGCTATAATTCTGTCTGTTCTTTCGATTTCTCAATTTCAATAGCCGATGGGAAGGGCTCCGAATCATTTTTTTTCTTGCTGTACTAAATTTTGAAAATCAAAGCTTCCTGGCTATTACGAAACTCGAACAGATAGAAAGTGTGGCCGTTGG
AATGAGGCTTGAGTAAAGTCAGCATGACCCTTGAAGGAGCAGCTTGTTCCCTCAGCTCCTGTTCCCTGTATTTCTCTTAACGCCGACGCAGAGGAGTAATGTAGTCCGTGTACCCAACAACTTCTATTGCCACACACCAAATCATTGACACCAATAGTCTTACGGTTCTCTGAGACGCACTTCTCATGTGCCATTCCGGT
GATAAAACTGATAAACTCTGTGACGCACTGCTGCATCAACCCCTTGGTTTCTGTCGACACCTTCGCATTCTTGCCCAACACTTTCTTTCATTATTCTGCTCACGATGGCCGACGGCATATACAACTCATTGGGGTTGTTGCTGTAATTACCACCACTGTTGGTGTTATTGTTGTGTATTTCTTTAAATACCGAAGCCGAG
GAGCTATGCAACACTGCTATTCTTGTTAGATTTCTGCGGAATGAGGCATTGCTATTCTGTCTGCGGAATACCGAAGCCGAGGAATGAAGGCTGCGGAGTGAGGGACTGCTATTCTGgtactagtttgttagatttcaaattctgttaggttgtttgatttgtcgatttcaaattctttttgttagttagatttctcaatt
tcaatggttttttttgtagATGAGCATTGCTATTCTGTCTG

Intron 2 GTAATCGTAG ... TTTTATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0.74	869	6	7
A2	0.61	1213	4	11
A3	0.75	1293	6	13.7
A4	0.59	1160	8	5.7
A5	0.36	1260	4	5
A6	4.96	313	12	20.7
A7	11.29	90	8	15
A8	3.01	91	3	2.7
A9	8.33	139	5	20.3
A10	9.67	24	0.7	4.7

>TRINITY_DN122221_c0_g1_i2 from sample A1 / Example spliced isoform

ATTTCTCAATTTCAATGGCCGATTGGAACAGCTCCGAAACAACTAAAAACGATAACATCAACACTGACGGTGAATTAGATTTCTCAATTTCAATGGCTGAGAGCGGTTTCAAATGCACCGATCAACACAACAATAACACCAACACTGGTGGTGATTACAGAAACAACACCAATGAGCTGTATATGCCGTAGGCTAGAGTT
TATCAGTTTTATCACCAAGAAGGTGCATGAGAAGTGTGCCACAGAGAGCCGTAAGACTCTTGGGGTCGATGATTTATTTTGGGGCAGTAGAAGTTGTTGGGTGCACAGACTGCATCACTCCTCGGCGTCGGTATTTAGAGAAATACAGGGAACGGGAGTTGAGCGAGCAAGCTGCTCCTTTAGAGGGTCATGGTGGCTTT
ACTCAACCTCATTCCAACGGCCATACTTTTTAAATGTTCGAGTTTCTCAAAAATTTTGTACAGTAGGAAGAAAATGATAAGGGATTTACTGTCTTGTTAATTGAATCTCAAAACAATCAAGGGCATTCAATTTTTTGTGAGGGCATTCTATTTTTTATGAGCTGAAATGGAATTGGGCTGCCTTATTTTTAGGCATTCCA
TTTTTTTTAACTGACATTCAACTTTTTAACTGCGCTCACCCATGGTCGCAGGTGGAATTGTCTCGTCATTTCATATGAGAGTTGATTCTCTGCCTCATCATTCTGGCACGAAATGAGAACCTACTTCTTGATGCAATCCTCCCAATTTACTTGCAAGCATTTTTGTAACAAGCTTTTAGCTGAGACCTTGTTGTAGTCAA
GTAAGCAGTGTGTAAAAGAACAAGTTTTAAGAGGTTAAATCACTAATTT

>TRINITY_DN122221_c0_g1_i1 from sample A1 / Example unspliced isoform:

ATTTCTCAATTTCAATGGCCGATTGGAACAGCTCCGAAACAACTAAAAACGATAACATCAACACTGACGgtaatcgtagcaacattcctaattcgatttctcaatttcaaatactgttactttgaagtttctcattttcgaattctagtagttcggaagatctttgattttcaaaagttttatgtagGTGAATTAGATTT
CTCAATTTCAATGGCTGAGAGCGGTTTCAAATGCACCGATCAACACAACAATAACACCAACACTGGTGGTGATTACAGAAACAACACCAATGAGCTGTATATGCCGTAGGCTAGAGTTTATCAGTTTTATCACCAAGAAGGTGCATGAGAAGTGTGCCACAGAGAGCCGTAAGACTCTTGGGGTCGATGATTTATTTTGG
GGCAGTAGAAGTTGTTGGGTGCACAGACTGCATCACTCCTCGGCGTCGGTATTTAGAGAAATACAGGGAACGGGAGTTGAGCGAGCAAGCTGCTCCTTTAGAGGGTCATGGTGGCTTTACTCAACCTCATTCCAACGGCCATACTTTTTAAATGTTCGAGTTTCTCAAAAATTTTGTACAGTAGGAAGAAAATGATAAGG
GATTTACTGTCTTGTTAATTGAATCTCAAAACAATCAAGGGCATTCAATTTTTTGTGAGGGCATTCTATTTTTTATGAGCTGAAATGGAATTGGGCTGCCTTATTTTTAGGCATTCCATTTTTTTTAACTGACATTCAACTTTTTAACTGCGCTCACCCATGGTCGCAGGTGGAATTGTCTCGTCATTTCATATGAGAGT
TGATTCTCTGCCTCATCATTCTGGCACGAAATGAGAACCTACTTCTTGATGCAATCCTCCCAATTTACTTGCAAGCATTTTTGTAACAAGCTTTTAGCTGAGACCTTGTTGTAGTCAAGTAAGCAGTGTGTAAAAGAACAAGTTTTAAGAGGTTAAATCACTAATTT

Intron 3 GTAATCGCAG ... TTTTCTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	4.83	4150	240	37.3
A2	5.3	6246	404	74
A3	5.08	6635.7	407.7	52.7
A4	4.86	5392.7	313.7	31
A5	5.6	6133.3	424.3	24.3
A6	7.18	818	72.3	150
A7	9.21	216.3	24	57.3
A8	5.61	241.7	16.3	53
A9	7.68	361.7	33.3	90
A10	9.73	59.3	7.3	22

>TRINITY_DN121577_c0_g2_i1 from sample A3 / Example spliced isoform

GTTAATTAGATTTCTGAATTTCAACAGTTTTCTGTAGGTGAGAATTGCTATAATTCTGTCTGTTCTTTCGATTTCTCAATTTCAATAGCCGATGGGAAGGGCTCCGAATCATTTTTTTTCTTGCTGTACTAAATTTTGAAAATCAAAGCTTCCTGGCTATTACGAAACTCGAACAGATAGAAAGTGTGGCCGTTGGAATG
AGGCTTGAGTAAAGTCAGCATGACCCTTGAAGGAGCAGCTTGTTCCCTCAGCTCCTGTTCCCTGTATTTCTCTTAACGCCGACGCAGAGGAGTAATGTAGTCCGTGTACCCAACAACTTCTATTGCCACACACCAAATCATTGACACCAATAGTCTTACGGTTCTCTGAGACGCACTTCTCATGTGCCATTCCGGTGATA
AAACTGATAAACTCTGTGACGCACTGCTGCATCAACCCCTTGGTTTCTGTCGACACCTTCGCATTCTTGCCCAACACTTTCTTTCATTATTCTGCTCACGATGGCCGACGGCATATACAACTCATTGGGGTTGTTGCTGTAATTACCACCACTGTTGGTGTTATTGTTGTGTATTTCTTTAAATACCGAAGCCGAGGAGC
TATGCAACACTGCTATTCTTGTTAGATTTCTGCGGAATGAGGCATTGCTATTCTGTCTGCGGAATACCGAAGCCGAGGAATGAAGGCTGCGGAGTGAGGGACTGCTATTCTGATGAGCATTGCTATTCTGTCTGTTTGTTCGATTTCTCAATTTCAATGGCCGATTGGAACAGCTCCGAAACAACTAAAAACGGCAACAT
CAACACTGTCGGTGAGTTAGATTTCTCAAATTTCAATGGCTGATGAGAGCGGTTTCAAATGAACTGATCAACACAACAATAACACCAACACTGGTGGTGATTACAAAAACAACACCAATGAGCTGTTTATGCCGTCGGCCAACGTGACCAGAATAATGAGGAAAGTGTTGAGCGAGGATGCGAAGTTGTCGACAGAAGCC
GAGGAGGTTGATGCAGCAGTGCGTGACAGAGTTTATGAGTTTTGTCACCGGGAAGGCGCACGAGAAGTGCGCCACAGAGAGCCGTAAGACTCTTGGGGTCGATGATTTGTTTTGGGGCAGTAGAAGTTGTTGGGTACACGGACTGCATCATTCCTCTGCGTCGGTATTTATAGAAATACAGGAAACGGGAGCTGAGCAAA
CAAGCTGCTGCTTCAGAGGGTCATGGTGGCTTTACTCAACCTCATTCCAACGGCCATACTTTTTATCTGTTCGAGTTTCTCAAAATTTTTGTACAGCAGGAAGAAAATGATAGGTGATTTACTGTCTTGTTGATTGCATCTCAAAACAATCAAGGGCATTCAATTTTTTGTGAGGGCATTCTATTTTTTAATGAGCTGAA
ATGGAATTGGGATGCCTTATTTTAGGGCATTCAATTATTTTTAACGACATTCAACTTTTTAAAGATTTTTCAGAAATGCTCAGATCCAACTTTTACCAATGTTCAAGAACAGATTCTTTAATTTTTAATTCAATTACATAGCTGAGTTCTTTCATGTCCTTGCTACTTTTTCAC

>TRINITY_DN121577_c0_g2_i4 from sample A3 / Example unspliced isoform:

ACGGCAACATCAACACTGTCGgtaatcgcagcaacattcctaattccatttctcaatttcaaatactgctactttgtcaagtttttcattttcgaattcttatattagtttggaagatctttgaatttcaaaagttttctgtagGTGAGTTAGATTTCTCAAATTTCAATGGCTGATGAGAGCGGTTTCAAATGAACTGA
TCAACACAACAATAACACCAACACTGGTGGTGATTACAAAAACAACACCAATGAGCTGTTTATGCCGTCGGCCAACGTGACCAGAATAATGAGGAAAGTGTTGAGCGAGGATGCGAAGTTGTCGACAGAAGCCGAGGAGGTTGATGCAGCAGTGCGTGACAGAGTTTATGAGTTTTGTCACCGGGAAGGCGCACGAGAAG
TGCGCCACAGAGAGCCGTAAGACTCTTGGGGTCGATGATTTGTTTTGGGGCAGTAGAAGTTGTTGGGTACACGGACTGCATCATTCCTCTGCGTCGGTATTTATAGAAATACAGGAAACGGGAGCTGAGCAAACAAGCTGCTGCTTCAGAGGGTCATGGTGGCTTTACTCAACCTCATTCCAACGGCCATACTTTTTATC
TGTTCGAGTTTCTCAAAATTTTTGTACAGCAGGAAGAAAATGATAGGTGATTTACTGTCTTGTTGATTGCATCTCAAAACAATCAAGGGCATTCAATTTTTTGTGAGGGCATTCTATTTTTTA

Intron 4 GTTCGAGTTT ... ATGGTCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	214	8
A2	99.66	1	297	9
A3	99.06	3	315	6
A4	100	0	196	11
A5	100	0	159	6
A6	100	0	3648	11
A7	99.26	8	1071	15
A8	99.78	3	1340	6
A9	99.63	6	1612	10
A10	99.37	3	473	6

>TRINITY_DN44319_c1_g6_i2 from sample A8 / Example spliced isoform

CAACGGCCATACTTTTTAAATGTGGAATTGTCTCGTCATTTCATATGAGAGTTGATTCTCTGCCTCATCATTCTGGCACGAAATGAGAACCTACTTCTTGATGCAATCCTCCCAATTTACTTGCAAGCATTTTTGTAACAAGCTTTTAGCTGAGACCTTGTTGTAGTCAAGTAAGCAGTGTGTAAAAGAACAAGTTTTAA
GAGGTTAAATCACTAATTTCTCAAATTTGGTCACTTTACTCAAGCAAGTTGTGACTCAGTTGGAGGCAACTACTGCAACATGGGTTAAGCTGGATGAACCATCTCTTGTAATGGATCTTGAGGCTCATAAATTGGAGGCCTTTTCCAAGGCATACTCTTACTGAGAAG

>TRINITY_DN44319_c1_g6_i1 from sample A8 / Example unspliced isoform:

CAGCTCCGAAACAACTAAAAACGATAACATCAACACTGACGGTGAATTAGATTTCTCAATTTCAATGGCTGAGAGCGGTTTCAAATGCACCGATCAACACAACAATAACACCAACACTGGTGGTGATTACAGAAACAACACCAATGAGCTGTATATGCCGTAGGCTAGAGTTTATCAGTTTTATCACCAAGAAGGTGCAT
GAGAAGTGTGCCACAGAGAGCCGTAAGACTCTTGGGGTCGATGATTTGTTTTGGGGCAGTAGAAGTTGTTGGGTGCACAGACTGCATCACTCCTCGGCGTCGGTATTTAGAGAAATACAGGGAACGGGAGTTGAGCGAGCAAGCTGCTCCTTTAGAGGGTCATGGTGGCTTTACTCAACCTCATTCCAACGGCCATACTT
TTTAAATgttcgagtttctcaaaaattttgtacagtaggaagaaaatgataagggatttactgtcttgttaattgaatctcaaaacaatcaagggcattcaattttttgtgagggcattctattttttatgagctgaaatggaattgggctgccttatttttaggcattccattttttttaactgacattcaacttttta
actgcgctcacccatggtcgcagGTGGAATTGTCTCGTCATTTCATATGAGAGTTGATTCTCTGCCTCATCATTCTGGCACGAAATGAGAACCTACTTCTTGATGCAATCCTCCCAATTTACTTGCAAGCATTTTTGTAACAAGCTTTTAGCTGAGACCTTGTTGTAGTCAAGTAAGCAGTGTGTAAAAGAACAAGTTTT
AAGAGGTTAAATCACTAATTTCTCAAATTTGGTCACTTTACTCAAGCAAGTTGTGACTCAGTTGGAGGCAACTACTGCAACATGGGTTAAGCTGGATGAACCATCTCTTGTAATGGATCTTGAGGCTCATAAATTGGAGGCCTTTTCCAAGGCATACTCTTACTGAGAAG