NA ----> NA

Intron 1 GTAAGTCCAG ... CTCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.29	6	1.8	1
A2	28.57	5	0	2
A3	50	4	0	4
A4	-1	0	0	0
A5	0	1	0	0
A6	44.44	15	4.2	12
A7	26.15	48	8.8	17
A8	34.2	55.8	11.6	29
A9	20.96	26.4	10.6	7
A10	9.68	28	7.4	3

>TRINITY_DN42952_c2_g1_i13 from sample A9 / Example spliced isoform

ACTACCTCGCACGCTCCGCTCTCAGTGTTCGGCGTTGGGAACGCTCTGTGGATTGAGCCATCCAGTTATCTTGTTTGATCTATGGATTGTATATGAGAGGGGAAATCGTGGCAAACTCACATTTGACGAACTCATGGTAGAATCACAGAAGGAAAAATCTTTGGATGGCATGGAGTTCAATTGGCGAATTGGAGTTCATT
CCTTCCTGGCCCAGGGTAGAGGTAGCGGCGAATACGACTGATATATTTGAGCAGGAAAATCGAAAACAGCTGGATCGTCTACAGAAGAAAATGACACAGAATGGAAGCAACTGAGGAGGCACTGTGGTGTTGATATTAATCACAGAATGGAAGCATGAGGAGGCCGGCACTGCGTTGAACGAATTGATGAGGCACGCTGT
TAAACAATTGAGGAGGCACTGTGTACAAGTAGCAGCTGCATCATGTGCACAGCTGGCTAGATCACATAGACACCCAATTGCAAAAGAAAATGAAAAGGCTGGATCGTTTACACACCCAATTGCAAAAGAAAATGAAAAGGCTGGATCGTTTACACACCCAATTGCAAAAGAAACAGGTGTTGACTGGCGTCTTCTCTTCT
GCGTGATCAGTTCACAACAGTGGCGATAATGGACAATGACCCTAGAGGATATTGGTGTGTGGTTGGCTATACGACTCTATTTAAACTTGGTTGAACTCTCGTTACTTGGAAATAAAGCCCAAGGATGAACTGGGGTTTATAGTTATAGTTTTATGTGTTCAAAATGTATTTGTATGGGTCCTGAGATGCCCTGCTGTGTA
ATGTTTATGAGGGTTTGATACCCTATCCAAATCTCTGCATTTTTAATATGGATATGGGTTCTTGTACAATCATACACTATCTGCGTTTTCTATTTGGAAATGAACTTGTATTTAACCCTATGAAGGGAATGTGTATCTATTTCTACATTTTTAAGTATTGTTGCTCCACTCTGTGATCTGCTCGAA

>TRINITY_DN42952_c2_g1_i6 from sample A9 / Example unspliced isoform:

ACTACCTCGCACGCTCCGCTCTCAGTGTTCGGCGTTGGGAACGCTCTGTGGATTGAGCCATCCAGTTATCTTGTTTGATCTATGGATTGTATATGAGAGGGGAAATCGTGGCAAACTCACATTTGACGAACTCATGGTAGAATCACAGAAGGAAAAATCTTTGGATGGCATGGAGTTCAATTGGCGAATTGGAGTTCATT
CCTTCCTGGCCCAGGGTAGAGGTAGCGGCGAATACGACTGATATATTTGAGCAGGAAAATCGAAAACAGCTGGATCGTCTACAGAAGAAAATGACACAGAATGGAAGCAACTGAGGAGGCACTGTGGTGTTGATATTAATCACAGAATGGAAGCATGAGGAGGCCGGCACTGCGTTGAACGAATTGATGAGGCACGCTGT
TAAACAATTGAGGAGGCACTGTGTACAAGTAGCAGCTGCATCATGTGCACAGCTGGCTAGATCACATAGACACCCAATTGCAAAAGAAAATGAAAAGGCTGGATCGTTTACACACCCAATTGCAAAAGAAAATGAAAAGGCTGGATCGTTTACACACCCAATTGCAAAAGAAACAGGTGTTGACTGGCGTCTTCTCTTCT
GCGTGATCAGTTCACAACAGTGGCGATAATGGACAATGgtaagtccagaaatttccaactcttagctcctccttttgcccatgtggggattactgttgatgtatgtttttattgggaactggtgaacttatttgagttcaatgtcacaagtttcgaatccctagaagtttcctttttggctatactgtggtgtaaatgta
tctatttgtctctttgcagACCCTAGAGGATATTGGTGTGTGGTTGGCTATACGACTCTATTTAAACTTGGTTGAACTCTCGTTACTTGGAAATAAAGCCCAAGGATGAACTGGGGTTTATAGTTATAGTTTTATGTGTTCAAAATGTATTTGTATGGGTCCTGAGATGCCCTGCTGTGTAATGTTTATGAGGGTTTGAT
ACCCTATCCAAATCTCTGCATTTTTAATATGGATATGGGTTCTTGTACAATCATACACTATCTGCGTTTTCTATTTGGAAATGAACTTGTATTTAACCCTATGAAGGGAATGTGTATCTATTTCTACATTTTTAAGTATTGTTGCTCCACTCTGTGATCTGCTCGAA

Intron 2 GTACCGTTTT ... AGTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	94.69	6	107	13
A2	91.3	14	147	12
A3	90.91	15	150	13
A4	94.19	5	81	11
A5	88.41	8	61	12
A6	92	65	747	51
A7	89.97	141	1265	107
A8	88.62	205	1597	131
A9	90.31	154	1435	94
A10	88.2	137	1024	60

>TRINITY_DN44043_c1_g2_i15 from sample A6 / Example spliced isoform

CATAGACACACAACTGCAAAAGAAAACGAAAAGGCTGGATCGTCTACACACCCAGTTGCAAAAGAAAATGAAACAGTTGGTGGCTAATGGTATTAGGAGAAGAGGTTGTCATATAACTCTAATTCTGCGATGGATTCTTGGTTAATGTTGGTCGGTTATTTACACTGATGTATTTACTACTGTCGGTGCGTAACGATTTT
AGATGTTCCCTTTTCTGTCGAAGTCAGGACGCGAGAGGATGCAAATATGGCTGGTTGGTTGGATGTTCCCTCTGGTTTCACATTAAATGAAAATTTATCTCCACCATTTGCCGTCTATTCT

>TRINITY_DN44043_c1_g2_i12 from sample A6 / Example unspliced isoform:

GGGATCGTCTACAGAAGAAAATGACACAGCACTGCGTTGGAGCAATTGAGGAGGCACTGTGTACAAGTAGCAGCTGGATCGCCTGCACAGCTGGCTAGATCACATAGACACAGAACTGCAAAAGAAAACGAAAAGGGTGGATCGTCTACACACCCAATTGCAAAAGAAAATGAAACAGgtaccgttttcctctcatggct
tcctaatataataaaaacaaatgaaatccatgtgttcaaaataatgtgtcatgtgtataaattctggaatctacattagattgatttattgtggcagagtagtttgcatagtctctacatatatgaattttctagtattcttcatacattctgtgttattttctctgttggttaacttatagttgtattcattggtgatg
tgaaaccaccttaatcaaaattataatacggagttctttaatgttaagaaattcgtatgagtaaactaagttttctaaattgattttattttaattttttttttgttaactgatagttgtattaattggtggtgtgaaaccacctaaatcaaaattataatagggagctctttataaactctaggatctagggttgctcc
ttgccattatgtggtgcctggttagttttacagTTGGTGGCTAATGGTATTAGGAGAAGAGGTTGTCATATAACTCTAATTCTGCGATGGATTCTTGGTTAATGTTGGTCGGTTATTTACACTGATGTATTTACTACTGTCGGTGCGTAACGATTTTAGATGTTCCCTTTTCTGTCGAAGTCAGGACGCGAGAGGATGCA
AATATGGCTGGTTGGTTGGATGTTCCCTCTGGTTTCACATTAAATGAAAATTTATCTCCACCATTTGCCGTCTATTCT

Intron 3 GTAAATGAAC ... TTAATCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.57	5	2	0
A2	25	12	4	2
A3	13.64	19	3	1
A4	0	9	0	0
A5	50	2	2	1
A6	8.51	43	4	2
A7	8.14	79	7	1
A8	6.42	102	7	0
A9	4.62	124	6	1
A10	0	82	0	0

>TRINITY_DN42952_c2_g1_i3 from sample A9 / Example spliced isoform

ACTACCTCGCACGCTCCGCTCTCAGTGTTCGGCGTTGGGAACGCTCTGTGGATTGAGCCATCCAGTTATCTTGTTTGATCTATGGATTGTATATGAGAGGGGAAATCGTGGCAAACTCACATTTGACGAACTCATGGTAGAATCACAGAAGGAAAAATCTTTGGATGGCATGGAGTTCAATTGGCGAATTGGAGTTCATT
CCTTCCTGGCCCAGGGTAGAGGTAGCGGCGAATACGACTGATATATTTGAGCAGGAAAATCGAAAACAGCTGGATCGTCTACAGAAGAAAATGACACAGAATGGAAGCAACTGAGGAGGCACTGTGTTGTTCATTCTAGGCCGGCACTACGTTGAAGGAATTGACGAGGCACGCAGCTGGATCGCTGGATCGCCTACGCA
GCTGGATGGCCTACACACCCAAATGCAGAAGAAAATGAAACAGATGTTCCCTTCTCTTTGGAAGGGAGGATGCGAGGGGATGCAAATCTGCCGTCTACTCGGTGTTATAATGATTTTAAATTTTATCTCCACCATTTGCCGTCTATTCTTGGTGTTATATTATATAATTAGGAGCAGAGGATGTCATATAATTATAGTTC
CACCGATGAATAATTTTTGCTCATGTAGAGTTGTGTAGTGCGAGTTTCCACGGACTTTTGTTATGTAGAATTATCGATTACGGCTTTTACTACACGTATGTTCTCCGAGCCAGTTTAGGAATACATAATCAAGGATCCGTCATCCGACGCCGTTTAAGAACTCGATGGACTGTGATCTTCTAAAACCCACCGAAATATTC
AAGTCAAAGTTAGGTCCAAATGATTAGTAAACTTGCCGTTTAGTTTTTTCTTACTATGTGGGAACTTTTCAGAAGATTTATGATATATAATTATCCACATAGGTTAGCTATTACTGAGATAAAGAATTAAAAAAAAAAAAAAAAAAGTGTTACTGCG

>TRINITY_DN42952_c2_g1_i5 from sample A9 / Example unspliced isoform:

ACTACCTCGCACGCTCCGCTCTCAGTGTTCGGCGTTGGGAACGCTCTGTGGATTGAGCCATCCAGTTATCTTGTTTGATCTATGGATTGTATATGAGAGGGGAAATCGTGGCAAACTCACATTTGACGAACTCATGGTAGAATCACAGAAGGAAAAATCTTTGGATGGCATGGAGTTCAATTGGCGAATTGGAGTTCATT
CCTTCCTGGCCCAGGGTAGAGGTAGCGGCGAATACGACTGATATATTTGAGCAGGAAAATCGAAAACAGCTGGATCGTCTACAGAAGAAAATGACACAGgtaaatgaacctaccgtttccctctcatagattttcaaatggaattttaattcatcaacgaaaattagattttgaattcttggtgttgtattttaatcaca
gAATGGAAGCAACTGAGGAGGCACTGTG