sp|Q02921|NO93_SOYBN ----> Early nodulin-93

Intron 1 GTGAGTCTTG ... TAACCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.64	21	0	5
A2	13.04	10	3	0
A3	27.27	4	1	2
A4	0	8	0	0
A5	7.69	12	0	2
A6	4.17	46	3	1
A7	17.14	58	9	15
A8	16.67	15	2	4
A9	12.2	36	1	9
A10	17.65	35	8	7

>TRINITY_DN48118_c1_g3_i13 from sample A7 / Example spliced isoform

CCAGCATCCATCTTCTTCCTTCATTTCTCATATATTATATTTATCTGATCTCAAGCATAGACTGGAGGGGGTTATTATAGCATAGCGGACGAGGAGATGACGAGTCCAGATCAACAAGCAGCTGACATGAAAATGCTCAGAGCAAAGCAGTGCTCCAATGAAGCAGTGAAAGCAGGGGCCAAGGCAGCTGCAATTGCATG
TGTAGCGTCGGCCATTCCCACATTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCGACAATCAGTGGCTCGGCGTATTTCATTGTAGCAGATAAGACCATTCTCAAGTCTGCACGTAGAAACTCCTTCCGTGAGCAAATGGAAAATCAGGTCGAGTACTGGC
CTTTCTGAAAGCAAACAGAGAATTAAATGACAAGCAGAGAGAGCAGAGCAGGGACAACAAATTTCTCTCTTTCAGATCTCAGATTCTCATGTACCCAGTCAGTTTGTAGATAATTAGGCAAATATATATGGACATGGATGGATGATGGAATAATTTCAAATCTCCAAGTCTTGTCATTTCATTTGCTCTGTTGTTTATCT
TAAAATGTAAATATAGGTAGTCTCAGTGCTCTTTCTGTTCGGCCTACTATCTCACTGCGTTTTCTGTTGGGCTCTGAATAGATTAGAGTAATAGGGGGCTTACCTCATCCTTCTCTATGCTATCTGTATAAGCAAATGAGTGCATGCAATATTAATATACAAACGTTGCT

>TRINITY_DN48118_c1_g3_i10 from sample A7 / Example unspliced isoform:

CCAGCATCCATCTTCTTCCTTCATTTCTCATATATTATATTTATCTGATCTCAAGCATAGACTGGAGGGGGTTATTATAGCATAGCGGACGAGGAGATGACGAGTCCAGATCAACAAGCAGCTGACATGAAAATGCTCAGAGCAAAGCAGTGCTCCAATGGTCTGAAATCATTCATTCATTTGCATTAATTTTTCAGATT
TTTTAGCACTGGCTATCCAGGTTATTTACTAGAGATCATGTTGATAAATTCATTGTCCTAATGGTGTTAAAATTACCATTTTTGGGGATGATGAAATTGATGAATGTATGTATATGACGTATATGCATACAGAAGCAGTGAAAGCAGGGGCCAAGGCAGCTGCAATTGCATGTGTAGCGTCGGCCATTCCCACAgtgagt
cttgttattgttgatgaatcaatcagtacccagtcaatttagtgacttgactaataatcatattcatgcaactgggcctacttattaactgcttggttggggtaatgtgaataacctacagTTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCGACAATCA
GTGGCTCGGCGTATTTCATTGTAGCAGATAAGACCATTCTCAAGTCTGCACGTAGAAACTCCTTCCGTGAGCAAATGGAAAATCAGGTCGAGTACTGGCCTTTCTGAAAGCAAACAGAGAATTAAATGACAAGCAGAGAGAGCAGAGCAGGGACAACAAATTTCTCTCTTTCAGATCTCAGATTCTCATGTACCCAGTCA
GTTTGTAGATAATTAGGCAAATATATATGGACATGGATGGATGATGGAATAATTTCAAATCTCCAAGTCTTGTCATTTCATTTGCTCTGTTGTTTATCTTAAAATGTAAATATAGGTAGTCTCAGTGCTCTTTCTGTTCGGCCTACTATCTCACTGCGTTTTCTGTTGGGCTCTGAATAGATTAGAGTAATAGGGGGCTT
ACCTCATCCTTCTCTATGCTATCTGTATAAGCAAATGAGTGCATGCAATATTAATATACAAACGTTGCT

Intron 2 GTAACCATTC ... GGATACACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.57	15	0	6
A2	23.08	10	0	3
A3	0	3	1	0
A4	18.75	13	0	3
A5	25	9	0	3
A6	17.39	38	1	8
A7	19.28	67	9	16
A8	15	17	0	3
A9	16.98	44	2	9
A10	0	37	2	0

>TRINITY_DN48118_c1_g3_i15 from sample A7 / Example spliced isoform

TTTGAGTTTCCCGTATCTAGCATTCATCTTCTTCTTCCTTAATTTTCATAAATTATATCTATCTGATCTCAAGCACTGAGTAAAGGGGATTATTAGCATAGCCGACGTAGAGATGACGAGTCCAGATCAACAACAAGCTGACATGAAAATTCTCAGAGCAAAGCAGTGCTCCAATGAAGCAGTGAAAGCAGGGGCCAAGG
CAGCTGCAATTGCATGCGTAGCGTCCGCCATCCCCACATTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCTACAATCACTGGTTCGGCCTATTTCATTGTAGCAGATAAGTCCATTCTCAACTCTGCAAGGAGAAACTCCTTCCGTGAACAAATGGAAAAT
CAAGCCGAGTACTGGCCTTTCTGATGGCAAGAACAGAACTATATTACAAGCAGAGAGCAGAGCAGAGACATCAAATCTCTCTCTTTCAGATCTCATGCACTCAGTCAGTCTGTTAATACTTAGGCAAACGTCCATGGAAATGGATGGATGGTGGAATAATCTCCCATCTCCAACCAAGTCTTTTGGTTTGCTCTGCTCTT
GGTCACAAAATGTAAATATAGGTAGTCTCCTATTTTTGCTGGGCTCTGAACAGATGAAGGCGCTCACCTTTTCCTTTTCTATGTTTGTCTGTACATGCAACTGAGTGCGTGTAATATTAATATATAACAATCGTTCACCATCAAGGCTAAGGCGTGGTTGCTAATCTCAA

>TRINITY_DN48118_c1_g3_i9 from sample A7 / Example unspliced isoform:

TTTGAGTTTCCCGTATCTAGCATTCATCTTCTTCTTCCTTAATTTTCATAAATTATATCTATCTGATCTCAAGCACTGAGTAAAGGGGATTATTAGCATAGCCGACGTAGAGATGACGAGTCCAGATCAACAACAAGCTGAAATGAAAATTCTCAGAGCAAAGCAGTGCTCCAATGgtaaccattcattcattcgcatta
atcttttttccttttttctgcattggctatggctagagatcatgtggctaaattcatttcatttgatttaaatgaaatcattgacatcattcctaaataacatgcagtgtgttaattcatttgtgggcatgatgaaactcatgaatgtatgtctgtatggatacacagAAGCAGTGAAAGCAGGGGCCAAGGCAGCTGCA
ATTGCATGCGTAGCGTCCGCCATCCCCACATTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCTACAATCACTGGTTCGGCCTATTTCATTGTAGCAGATAAGTCCATTCTCAACTCTGCAAGGAGAAACTCCTTCCGTGAACAAATGGAAAATCAAGCCGA
GTACTGGCCTTTCTGATGGCAAGAACAGAACTATATTACAAGCAGAGAGCAGAGCAGAGACATCAAATCTCTCTCTTTCAGATCTCATGCACTCAGTCAGTCTGTTAATACTTAGGCAAACGTCCATGGAAATGGATGGATGGTGGAATAATCTCCCATCTCCAACCAAGTCTTTTGGTTTGCTCTGCTCTTGGTCACAA
AATGTAAATATAGGTAGTCTCCTATTTTTGCTGGGCTCTGAACAGATGAAGGCGCTCACCTTTTCCTTTTCTATGTTTGTCTGTACATGCAACTGAGTGCGTGTAATATTAATATATAACAATCGTTCACCATCAAGGCTAAGGCGTGGTTGCTAATCTCAA

Intron 3 GTGAGTCTTA ... TAATCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.5	21	3	1
A2	0	10	0	0
A3	0	4	0	0
A4	11.11	8	1	0
A5	25	12	4	0
A6	11.11	48	6	0
A7	1.82	54	1	2
A8	7.14	13	1	0
A9	5.41	35	2	0
A10	8.33	33	3	0

>TRINITY_DN48118_c1_g3_i7 from sample A7 / Example spliced isoform

TCATCTTCTTCTTCGTCATTTTCATAAATCAATTTATCTGATCTCAAGCACTGAGCGAAGGGATTTATTATAACATAGCTTATGAGGAGATGCCGAGTTCACATATTCAACAAGCGGACATGAAAATTCTCAGAGCCAAGCAGTGCTCCAATGAAGCAGTGAAAGCAGGGGCCAAGGCAGCTGCAATTGCATGCGTAGCG
TCGGCCATCCCCACATTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCTACAATCACTGGTTCGGCCTATTTCATTGTAGCAGATAAGTCCATTCTCAACTCTGCAAGGAGAAACTCCTTCCGTGAACAAATGGAAAATCAAGCCGAGTACTGGCCTTTCTG
ATGGCAAGAACAGAACTATATTACAAGCAGAGAGCAGAGCAGAGACATCAAATCTCTCTCTTTCAGATCTCATGCACTCAGTCAGTCTGTTAATACTTAGGCAAACGTCCATGGAAATGGATGGATGGTGGAATAATCTCCCATCTCCAACCAAGTCTTTTGGTTTGCTCTGCTCTTGGTCACAAAATGTAAATATAGGT
AGTCTCCTATTTTTGCTGGGCTCTGAACAGATGAAGGCGCTCACCTTTTCCTTTTCTATGTTTGTCTGTACATGCAACTGAGTGCGTGTAATATTAATATATAACAATCGTTCACCATCAAGGCTAAGGCGTGGTTGCTAATCTCAA

>TRINITY_DN48118_c1_g3_i1 from sample A7 / Example unspliced isoform:

TCATCTTCTTCTTCGTCATTTTCATAAATCAATTTATCTGATCTCAAGCACTGAGCGAAGGGATTTATTATAACATAGCTTATGAGGAGATGCCGAGTTCACATATTCAACAAGCGGACATGAAAATTCTCAGAGCCAAGCAGTGCTCCAATGAAGCAGTGAAAGCAGGGGCCAAGGCAGCTGCAATTGCATGCGTAGCG
TCGGCCATCCCCACAgtgagtcttattattgttactaatctgtacccattcaattcactggcttaaccaattttcatattcttgcaatcgggcctacttattaactgctggattgggttgatgtataacgcggtgttaataatctgcagTTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCT

Intron 4 GTCTGAAATC ... ATGCATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	8.34	15	0	3
A2	29.63	10	6	2.5
A3	17.14	2.5	0	1
A4	6.67	13	0	2
A5	5	9	0	1
A6	13.92	38.5	7	5.5
A7	13.96	66	14.5	7
A8	2.78	17	0	1
A9	9.49	42	3	6
A10	15.39	39.5	11	3.5

>TRINITY_DN48118_c1_g3_i13 from sample A7 / Example spliced isoform

CCAGCATCCATCTTCTTCCTTCATTTCTCATATATTATATTTATCTGATCTCAAGCATAGACTGGAGGGGGTTATTATAGCATAGCGGACGAGGAGATGACGAGTCCAGATCAACAAGCAGCTGACATGAAAATGCTCAGAGCAAAGCAGTGCTCCAATGAAGCAGTGAAAGCAGGGGCCAAGGCAGCTGCAATTGCATG
TGTAGCGTCGGCCATTCCCACATTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCGACAATCAGTGGCTCGGCGTATTTCATTGTAGCAGATAAGACCATTCTCAAGTCTGCACGTAGAAACTCCTTCCGTGAGCAAATGGAAAATCAGGTCGAGTACTGGC
CTTTCTGAAAGCAAACAGAGAATTAAATGACAAGCAGAGAGAGCAGAGCAGGGACAACAAATTTCTCTCTTTCAGATCTCAGATTCTCATGTACCCAGTCAGTTTGTAGATAATTAGGCAAATATATATGGACATGGATGGATGATGGAATAATTTCAAATCTCCAAGTCTTGTCATTTCATTTGCTCTGTTGTTTATCT
TAAAATGTAAATATAGGTAGTCTCAGTGCTCTTTCTGTTCGGCCTACTATCTCACTGCGTTTTCTGTTGGGCTCTGAATAGATTAGAGTAATAGGGGGCTTACCTCATCCTTCTCTATGCTATCTGTATAAGCAAATGAGTGCATGCAATATTAATATACAAACGTTGCT

>TRINITY_DN48118_c1_g3_i10 from sample A7 / Example unspliced isoform:

CCAGCATCCATCTTCTTCCTTCATTTCTCATATATTATATTTATCTGATCTCAAGCATAGACTGGAGGGGGTTATTATAGCATAGCGGACGAGGAGATGACGAGTCCAGATCAACAAGCAGCTGACATGAAAATGCTCAGAGCAAAGCAGTGCTCCAATGgtctgaaatcattcattcatttgcattaatttttcagatt
ttttagcactggctatccaggttatttactagagatcatgttgataaattcattgtcctaatggtgttaaaattaccatttttggggatgatgaaattgatgaatgtatgtatatgacgtatatgcatacagAAGCAGTGAAAGCAGGGGCCAAGGCAGCTGCAATTGCATGTGTAGCGTCGGCCATTCCCACAGTGAGT
CTTGTTATTGTTGATGAATCAATCAGTACCCAGTCAATTTAGTGACTTGACTAATAATCATATTCATGCAACTGGGCCTACTTATTAACTGCTTGGTTGGGGTAATGTGAATAACCTACAGTTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCGACAATCA
GTGGCTCGGCGTATTTCATTGTAGCAGATAAGACCATTCTCAAGTCTGCACGTAGAAACTCCTTCCGTGAGCAAATGGAAAATCAGGTCGAGTACTGGCCTTTCTGAAAGCAAACAGAGAATTAAATGACAAGCAGAGAGAGCAGAGCAGGGACAACAAATTTCTCTCTTTCAGATCTCAGATTCTCATGTACCCAGTCA
GTTTGTAGATAATTAGGCAAATATATATGGACATGGATGGATGATGGAATAATTTCAAATCTCCAAGTCTTGTCATTTCATTTGCTCTGTTGTTTATCTTAAAATGTAAATATAGGTAGTCTCAGTGCTCTTTCTGTTCGGCCTACTATCTCACTGCGTTTTCTGTTGGGCTCTGAATAGATTAGAGTAATAGGGGGCTT
ACCTCATCCTTCTCTATGCTATCTGTATAAGCAAATGAGTGCATGCAATATTAATATACAAACGTTGCT

Intron 5 GTCAGTAATT ... TGATTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	5.5	0	0
A2	0	6	0	0
A3	0	6	0	0
A4	42.86	2	0	3
A5	0	5	0	0
A6	2.19	22.5	0	1
A7	13.3	42.5	2	11
A8	28	9	4	3
A9	10.55	25.5	0	6
A10	15	25.5	4	5

>TRINITY_DN39404_c1_g1_i2 from sample A8 / Example spliced isoform

TATCTGTCTGATCTCAAGCACTGAGTAGAGGGGATTATTAGCATAGCCGACGTAGAGATGACGAGTCCAGATCAACAACAAGCTGACATGAAAATTCTCAGAGCAAAGCAGTGCTCCAATGAAGCAGTGAAAGCAGGGGCCAAGGCAGCTGCAATTGCATGCGTAGCGTCCGCCATCCCCACATTGCTGGGGGCAAGGGC
GATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCTACAATCACTGGTTCGGCCTATTTCATTGTAGCAGATAAGTCCATTCTCAACTCTGCAAGGAGAAACTCCTTCCGTGAACAAATGGAAAATCAAGCCGAGTACTGGCCTTTCTGATGGCAAGAACAGAACTATATTACAAGCAGAG
AGCAGAGCAGAGACATCAAATCTCTCTCTTTCAGATCTCATGCACTCAGTCAGTCTGTTAATACTTAGGCAAACGTCCATGGAAATGGATGGATGGTGGAATAATCTCCCATCTCCAACCAAGTCTTTTGGTTTGCTCTGCTCTTGGTCACAAAATGTAAATATAGGTAGTCTCCTATTTTTGCTGGGCTCTGAACAGAT
GAAGGCGCTCACCTTTTCCTTTTCTATGTTTGTCTGTACATGCAACTGAGTGCGTGTAATATTAATATATAACAATCGTTCACCATCAAGGCTAAGGCGTGGTTGCTAATCTCAAATTTTGTGGTTTGATGACTAAAATTTCAATGGAATTGCATGTGGGCCATATGAAATTCCATCAAAATTCCAAAATTCCTTTTCTG
GATAAGTGTTATAGAACAAAGCAAATAGTTTTCATATAAACATATCAGCCAATGCACAAAACTTTGTATCAATACTCCAATCAAGAAGGATGTCTTTCTCTG

>TRINITY_DN39404_c1_g1_i7 from sample A8 / Example unspliced isoform:

GTTATTGTCAATGAAGCAATCAATCAGTACAGATTCAATTCATTGGCTTCATTAATAATCATATTCGTGCAACTGGGCCGACTAATTTCTTGCTGGGTTGGGTTAATGTTAAAAATCTGCAGTTGCTGGGAGCAAGGGCTATTCCTTGGGCAAGGGCTAACCTCAACCATGCTGCACAGGCGCTCATTATCTCTACAAgt
cagtaattaattaaatattgttcgatgtcattccaaatcttaggttgcagttgcttaattatcctcaatatctattcattttttggcatgacatatttgcagagtacatgaaactactactattatatttagctactttttactaaaatgtttgcattgcaatgcattgatacatgttctgataaaggattttacaatta
ctgaattgatttggctgatatccctcattataatgatctcaggaaactgcatattgatgttgtgttgtgtgtactttgattggcagTCACTGGTTCGGCCTATTTCATTGTAGCAGATAAGTCCATTCTCAACTCTGCAAGGAGAAACTCCTTCCGTGAACAAATGGAAAATCAAGCCGAGTACTGGCCTTTCTGATGGC
AAGAACAGAACTATATTACAAGCAGAGAGCAGAGCAGAGACATCAAATCTCTCTCTTTCAGATCTCATGCACTCAGTCAGTCTGTTAATACTTAGGCAAACGTCCATGGAAATGGATGGATGGTGGAATAATCTCCCATCTCCAACCAAGTCTTTTGGTTTGCTCTGCTCTTGGTCACAAAATGTAAATATAGGTAGTCT
CCTATTTTTGCTGGGCTCTGAACAGATGAAGGCGCTCACCTTTTCCTTTTCTATGTTTGTCTGTACATGCAACTGAGTGCGTGTAATATTAATATATAACAATCGTTCACCATCAAGGCTAAGGCGTGGTTGCTAATCTCAAATTTTGTGGTTTGATGACTAAAATTTCAATGGAATTGCATGTGGGCCATATGAAATTC
CATCAAAATTCCAAAATTCCTTTTCTGGATAAGTGTTATAGAACAAAGCAAATAGTTTTCATATAAACATATCAGCCAATGCACAAAACTTTGTATCAATACTCCAATCAAGAAGGATGTCTTTCTCTG