NA ----> NA

Intron 1 GTATGCCACC ... AATTATAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.14	284	79	55.5
A2	18.95	307	96	47.5
A3	20.77	433	137	90
A4	21.58	288	99	59.5
A5	20.88	197	63	41
A6	17.81	3446	970	523.5
A7	17.56	3824	1116	513
A8	18.12	2782.5	805	426.5
A9	18.08	3861.5	1180	524
A10	18.28	3417.5	1066	463

>TRINITY_DN131486_c8_g1_i4 from sample A1 / Example spliced isoform

TGTCACGGTGAGAAGTACTTGTTTAAATTGGCAGGAGAAAGTTGCATTTAAAGACGTGAAAACTGAATGGGTTGAAGATAGGTATTCGGAATGGGGCCGACATAACGGGAAGGGTCAGATGAACATGGCAGCGTGTTAAACTGTGTCTGTCTAATTTGTGTCGAGGATACAACTTACCATCGTCGGCGGCGTCATGAAGC
ATAAGAAAATTGCAGGGCCAGGGGCAGGGGCTGGGCCAGGAGCAAGATCGAAAATAGAGGCTCCAGGAGCGACCACTGCTTCTTCTTCATCAACATCATCTCAGAACCCATCTTTCATCCGGCGTTACAGATTTGCATGGCCAGCTTTACTAGGACTAAATTTGGTCGTTGGCGGTGAGTTGTTGGCTTGTTTATTTTAC
GTTATGAGGGTTTCTCTTTCTGAGATTGCTATCTGTTATCATTTCTTTTCTCATGTTATCTCACACTCGATCTGTACCCACAGTTTTGATATGTAACTTGACCTAAGCTAATTCTGTCTGCTTAACAAATTATCGAGGAAAGCTTTACATCAAAGATGATAGTAGAAAGTGTTTGAATGCAGAAACCTAAACTATCTACA
CAGGTGGGTGAGGTGACTAGATTGCTCTGATGATTACTCTTGGCCCAACATAATCAGGCTTTCATTTATAGAAGTTTTCTCAACTCAGCCGGGGGAGCATTATGGTTGCCGGCTATGGATGGTTGCTAAACTCTAGTATTCACATATTTAACTAAGGTGTAACAATACCCTATATACACAGGAACAACACAAAACCAGAT
CAAGGAAGAGTGGATTATAATGGAGTGCTTATCGTTTAGTACTAAGTTGCACGAAATATGGAAATGGGTACGGTTTCACGGAAAGGGATATGGTTGCCCAAACATAGTGGAACAAATTATTTAAAATCTTGTTTATATACAAAATAGCATTGCATCAGGCAATGTCCAAAAGTAGTCTATGCCAAGGCTCAAAATATAAA
ATGAGCTACACAAAAGTCATTTAGCTCTCAAAAGCCGGAGACAATAAACTTAGGGTTGTAATTATTAGGAGTTTCCTGCAACTTAGGGTTAAACTCTACTGAAAAATTTAAAAAACCCAAAATGTCCTCCTAGTCCAAACCACCTGCCTTGCTATGAATGGACTTAGGGTTTGATATCTGCCGAAATATGTAGCATGGTC
CCTTTTTAAACAAAAACTATATATTTGTTATTTCAAGAGTAAAATCAAAATATATATTGCCGAGCCTCAAAGCTGTTTCACAAAAACATAAAAAGGCCTTCAGCCTGAGCCACCCCTGTAATTACTTATTACAGTCCTCCAGTTCTAAAAGGCCCAGACTTTCAGAACACCTTTTCTAAGAAAAGGCCATACAGTCTTGT
AAGATTACCATCTTCCACCTTGTTCACAGGTTACAGAATGGATAAGGAAATTTCTGATGAGGCCTGACTACCCCTGAACTCTTGGAAGAGAACACCTTCCTGCAGACTAATGCATCCTTCGAGAGAGAGTCTGCTTTTTGATTGAATTCTCTGAAGATATGGGAGAAAGATATAGTGTCAAACCAGGATTTGGCCTCCAT
AACTCTGCTCATTATAG

>TRINITY_DN131486_c8_g1_i3 from sample A1 / Example unspliced isoform:

TGTCACGGTGAGAAGTACTTGTTTAAATTGGCAGGAGAAAGTTGCATTTAAAGACGTGAAAACTGAATGGGTTGAAGATAGGTATTCGGAATGGGGCCGACATAACGGGAAGGGTCAGATGAACATGGCAGCGTGTTAAACTGTGTCTGTCTAATTTGTGTCGAGGATACAACTTACCATCGTCGGCGGCGTCATGAAGC
ATAAGAAAAgtatgccaccctccatttttccttcaatttttgtattgtttaacaaatggaatgaaacaacatgagtgagctgattcatataattataaagTTGCAGGGCCAGGGGCAGGGGCTGGGCCAGGAGCAAGATCGAAAATAGAGGCTCCAGGAGCGACCACTGCTTCTTCTTCATCAACATCATCTCAGAACCC
ATCTTTCATCCGGCGTTACAGATTTGCATGGCCAGCTTTACTAGGACTAAATTTGGTCGTTGGCGGTTATGTCTTGCTTAGAACAAAACCAAAACATGCAAATTTGGATGAAGCCAAGGAACGAGTTCAAGATGCTGAAACCGAGACAGTCACTCAACCTGTCTCAAGCGCCACATCACCTTCTGTGATTCCAGTTGCGA
GTGGGCAGATTTCTGAAGATGAACAGCGACAGCTATTCAAGTGGATCTTGGAGGAGAAGAGACAGATAAAAGCAGCAAACCCATCAGAGAAGAGCCAAATTGATGAAGAGAAAGCTATTCTTAAGCAATATATTACCAGCAAAGAAATTCGTAATTTCTAGAAAATACTAATTGTCGACACATCTGCAAAGATTTTTTTA
CTCATGTTACAATTTAGCATGGTGATACCTTTCAGCCATAGTTAACTTTGTATTGTGTGAATACTTGAGCATAACTTTGACAAGCGGATTTGATATTTCAGTTTTTGATTCATTCTGATTTTCCATCTTTAAATGGTAACATTGTGACAGCTGTGTGGAAACTGAGTTGCGACCTATATGACATTTCATTTGGCACATTT
GTTTCTTTAATTTTGGTTATGATTTGTATACAGCAGCATAGATCCATCTTAACCCTGAACCTATTAATCACTGAGAGGAATTAAAAATAAACCAAGATGCAAACCTGTTGGTCTCAAGATGAATTTATGCTGGTTTATTTCATTATATGTTTTTCCTCATGTAAAAAATCATCTCTTTTGATTAGTCAAAATAAAAAAGA
TACATTTAGATAATTTACTCATCAAGCGGCATAAAATGCCTATGAGCTGAAACATGCCTGGCATAAGCAATCTGTATGTCA

Intron 2 GCCTAGGGGA ... TTGTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.62	232	57	71
A2	23.04	172	57	46
A3	23.52	239	86	61
A4	24.9	193	57	71
A5	20.51	124	31	33
A6	20.34	2092	608	460
A7	20.61	2126	646	458
A8	21.83	1946	624	463
A9	21.18	2267	681	537
A10	21.35	1917	585	456

>TRINITY_DN44922_c0_g3_i3 from sample A10 / Example spliced isoform

ATCAATGTCACGGTGAGAAGTACTTGTTTAAATTGGCAGGAGAAAGTTGCATTTAAAGACGTGAAAACTGAATGGGTTGAAGATAGGTATTCGGAATGGGGCCGACATAACGGGAAGGGTCAGATGAACATGGCAGCGTGTTAAACTGTGTCTGTCTAATTTGTGTCGAGGATACAACTTACCATCGTCGGCGGCGTCAT
GAAGCATAAGAAAATTGCAGGGCCAGGGGCAGGGGCTGGGTCAGGAGCAAGATCGAAAATAGAGGCTCCAGGAGCGACCACTGCTTCTTCTTCATCAACATCATCTCAGAACCCATCTTTCATCCGGCGTTACAGATTTGCATGGCCAGCTTTACTAGGACTAAATTTGGTCGTTGGCGGTTATGTCTTGCTTAGAACAA
AACCAAAACATGCAAATTTGGATGAAGCCAAGGAACGAGTTCAAGATGCTGAAACCGAGACAGTCACTCAACCTGTCTCAAGCGCCACATCACCTTCTGTGATTCCAGTTGCGAGTGGGCAGATTTCTGAAGATGAACAGCGACAGCTGTTCAAGTGGATCTTGGAGGAGAAGAGACAGATAAAAGCAGCAAACCCATCA
GAGAAGAGCCGAATTGATGAAGAGAAAGCTATTCTTAAGCAATATATTACCAGCAAAGAAATTCGTAATTTCTAGAAAATACTAATTGTCGACACATCTGCAAAGATTTTTTTACTCATGTTACAATTTAGCATGGTGATACCTTTCAGCCATAGTTAACTTTGTATTGTGTGAATACTTGAGCATAACTTTGACAAGCG
GATTTGATATTTCAGTTTTTGATTCATTCTGATTTTCCATCTTTAAATGGTAACATTGTGACAGCTGTGTGGAAACTGAGTTGCGACCTATATGACATTTCATTTGGCACATTTGTTTCTTTAATTTTGGTTATGATTTGTATACAGCAGCATAGATCCATCTTAACCCTGAATCTATTAATCACTGAGAGGAATTAAAA
ATAAACCAAGATGCAAACCTGTTGGTCTCAAGATGAATTTATGCTGGA

>TRINITY_DN44922_c0_g3_i1 from sample A10 / Example unspliced isoform:

ATCAATGTCACGGTGAGAAGTACTTGTTTAAATTGGCAGGAGAAAGTTGCATTTAAAGACGTGAAAACTGAATGGGTTGAAGATAGGTATTCGGAATGGGGCCGACATAACGGGAAGGGTCAGATGAACATGGCAGCGTGTTAAACTGTGTCTGTCTAATTTGTGTCGAGGATACAACTTACCATCGTCGGCGGCGTCAT
GAAGCATAAGAAAATTGCAGGGCCAGGGGCAGGGGCTGGGTCAGGAGCAAGATCGAAAATAGAGGCTCCAGGAGCGACCACTGCTTCTTCTTCATCAACATCATCTCAGAACCCATCTTTCATCCGGCGTTACAGATTTGCATGGCCAGCTTTACTAGGACTAAATTTGGTCGTTGGCGgcctaggggactttcagcctg
tcacccatgcctggttccaccgtttcatcattttgttccatttgtattgaggctttttttcaatttctacatattggattcttgttttcagGTTATGTCTTGCTTAGAACAAAACCAAAACATGCAAATTTGGATGAAGCCAAGGAACGAGTTCAAGATGCTGAAACCGAGACAGTCACTCAACCTGTCTCAAGCGCCAC
ATCACCTTCTGTGATTCCAGTTGCGAGTGGGCAGATTTCTGAAGATGAACAGCGACAGCTGTTCAAGTGGATCTTGGAGGAGAAGAGACAGATAAAAGCAGCAAACCCATCAGAGAAGAGCCGAATTGATGAAGAGAAAGCTATTCTTAAGCAATATATTACCAGCAAAGAAATTCGTAATTTCTAGAAAATACTAATTG
TCGACACATCTGCAAAGATTTTTTTACTCATGTTACAATTTAGCATGGTGATACCTTTCAGCCATAGTTAACTTTGTATTGTGTGAATACTTGAGCATAACTTTGACAAGCGGATTTGATATTTCAGTTTTTGATTCATTCTGATTTTCCATCTTTAAATGGTAACATTGTGACAGCTGTGTGGAAACTGAGTTGCGACC
TATATGACATTTCATTTGGCACATTTGTTTCTTTAATTTTGGTTATGATTTGTATACAGCAGCATAGATCCATCTTAACCCTGAATCTATTAATCACTGAGAGGAATTAAAAATAAACCAAGATGCAAACCTGTTGGTCTCAAGATGAATTTATGCTGGA

Intron 3 GCCTAGGGGA ... TTGTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.62	232	57	71
A2	23.04	172	57	46
A3	23.52	239	86	61
A4	24.9	193	57	71
A5	20.51	124	31	33
A6	20.35	2091	608.3	460
A7	20.61	2126	646	458
A8	21.83	1946	624	463
A9	21.18	2267	681	537
A10	21.39	1917	585.3	458

>TRINITY_DN107847_c0_g1_i1 from sample A2 / Example spliced isoform

ATCAATGTCACGGTGAGAAGTACTTGTTTAAATTGGCAGGAGAAAGTTGCATTTAAAGACGTGAAAACTGAATGGGTTGAAGATAGGTATTCGGAATGGGGCCGACATAACGGGAAGGGTCAGATGAACATGGCAGCGTGTTAAACTGTGTCTGTCTAATTTGTGTCGAGGATACAACTTACCATCGTCGGCGGCGTCAT
GAAGCATAAGAAAATTGCAGGGCCAGGGGCAGGGGCTGGGTCAGGAGCAAGATCGAAAATAGAGGCTCCAGGAGCGACCACTGCTTCTTCTTCATCAACATCATCTCAGAACCCATCTTTCATCCGGCGTTACAGATTTGCATGGCCAGCTTTACTAGGACTAAATTTGGTCGTTGGCGGTTATGTCTTGCTTAGAACAA
AACCAAAACATGCAAATTTGGATGAAGCCAAGGAACGAGTTCAAGATGCTGAAACCGAGACAGTCACTCAACCTGTCTCAAGCGCCACATCACCTTCTGTGATTCCAGTTGCGAGTGGGCAGATTTCTGAAGATGAACAGCGACAGCTGTTCAAGTGGATCTTGGAGGAGAAGAGACAGATAAAAGCAGCAAACCCATCA
GAGAAGAGCCGAATTGATGAAGAGAAAGCTATTCTTAAGCAATATATTACCAGCAAAGAAATTCGTAATTTCTAGAAAATACTAATTGTCGACACATCTGCAAAGATTTTTTTACTCATGTTACAATTTAGCATGGTGATACCTTTCAGCCATAGTTAACTTTGTATTGTGTGAATACTTGAGCATAACTTTGACAAGCG
GATTTGATATTTCAGTTTTTGATTCATTCTGATTTTCCATCTTTAAATGGTAACATTGTGACAGCTGTGTGGAAACTGAGTTGCGACCTATATGACATTTCATTTGGCACATTTGTTTCTTTAATTTTGGTTATGATTTGTATACAGCAGCA

>TRINITY_DN107847_c0_g1_i3 from sample A2 / Example unspliced isoform:

ATCAATGTCACGGTGAGAAGTACTTGTTTAAATTGGCAGGAGAAAGTTGCATTTAAAGACGTGAAAACTGAATGGGTTGAAGATAGGTATTCGGAATGGGGCCGACATAACGGGAAGGGTCAGATGAACATGGCAGCGTGTTAAACTGTGTCTGTCTAATTTGTGTCGAGGATACAACTTACCATCGTCGGCGGCGTCAT
GAAGCATAAGAAAATTGCAGGGCCAGGGGCAGGGGCTGGGTCAGGAGCAAGATCGAAAATAGAGGCTCCAGGAGCGACCACTGCTTCTTCTTCATCAACATCATCTCAGAACCCATCTTTCATCCGGCGTTACAGATTTGCATGGCCAGCTTTACTAGGACTAAATTTGGTCGTTGGCGgcctaggggactttcagcctg
tcacccatgcctggttccaccgtttcatcattttgttccatttgtattgaggcttttttcaatttctacatattggattcttgttttcagGTTATGTCTTGCTTAGAACAAAACCAAAACATGCAAATTTGGATGAAGCCAAGGAACGAGTTCAAGATGCTGAAACCGAGACAGTCACTCAACCTGTCTCAAGCGCCACA
TCACCTTCTGTGATTCCAGTTGCGAGTGGGCAGATTTCTGAAGATGAACAGCGACAGCTGTTCAAGTGGATCTTGGAGGAGAAGAGACAGATAAAAGCAGCAAACCCATCAGAGAAGAGCCGAATTGATGAAGAGAAAGCTATTCTTAAGCAATATATTACCAGCAAAGAAATTCGTAATTTCTAGAAAATACTAATTGT
CGACACATCTGCAAAGATTTTTTTACTCATGTTACAATTTAGCATGGTGATACCTTTCAGCCATAGTTAACTTTGTATTGTGTGAATACTTGAGCATAACTTTGACAAGCGGATTTGATATTTCAGTTTTTGATTCATTCTGATTTTCCATCTTTAAATGGTAACATTGTGACAGCTGTGTGGAAACTGAGTTGCGACCT
ATATGACATTTCATTTGGCACATTTGTTTCTTTAATTTTGGTTATGATTTGTATACAGCAGCA