sp|O80822|PER25_ARATH ----> Peroxidase 25

Intron 1 GTCGGCGTAG ... TGGAAAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	134	268	20
A2	69.33	100	226	14
A3	71.53	125	314	14
A4	70.17	88	207	7
A5	70.39	69	164	4
A6	62.92	290	492	32
A7	64.78	318	585	32
A8	58.31	286	400	38
A9	67.6	334	697	38
A10	65.72	314	602	30

>TRINITY_DN43464_c2_g1_i12 from sample A8 / Example spliced isoform

CCGGAAACGCAAGAAAGAGATGGTGGTTATAGATTTGCTTAGATTGGTCCATAAGATAAATCTGGCCTTTCAAATTTAAAATGAAAGCCGTTAGAAAAGAAAGAGAGAAGTGGAAGCCCGGAAAAGCAAGAAAGAGAGAAGTGGAAGACCAGAAAAGCAAGAAAGAGAAGGTAGTTATAGATTTGCTTAGATAGCACAGG
CATTCAAAATATTGAGTATCGATTTGTAGTGTGCTGTGTGTCTTTCTTGAAGAGGAGGGTGGCCGCATATTCTCCATCTGTGTTGGAGTGGAAGCAAAGGGAACCAAAGTTGTTTTCTACTACCAAGATCTGTCACACAGCAGAGAATATCGTCAGATCCACAGTTGAAGCCCATGTGAAGGAGGGCAGAGGCACTGCTA
GTATCGGCTCTACAACTTCACTTCCACGGGGAACGCCGACCCGACAATAAACTC

>TRINITY_DN43464_c2_g1_i15 from sample A8 / Example unspliced isoform:

CCGGAAACGCAAGAAAGAGATGGTGGTTATAGATTTGCTTAGATTGGTCCATAAGATAAATCTGGCCTTTCAAATTTAAAATGAAAGCCGTTAGAAAAGAAAGAGAGAAGTGGAAGCCCGGAAAAGCAAGAAAGAGAGAAGTGGAAGACCAGAAAAGCAAGAAAGAGAAGGTAGTTATAGATTTGCTTAGATAGCACAGG
CATTCAAAATATTGAGTATCGATTTGTAGTGTGCTGTGTGTCTTTCTTGAAGAGGAGGGTGGCCGCATATTCTCCATCTGTGTTGGAGTGGAAGCAAAGGGAACCAAAGTTGTTTTCTACTACCAAGATCTGTCACACAGCAGAGAATATCGTCAGATCCACAGTTGAAGCCCATGTGAAGgtcggcgtaggacttgctc
ccggaatttgttttcatgcgaactattctttgaaacctgcagtttttagtggtccatgattcccagtcattcccggattagttgctgaagataattgcttctgctgcttcttcttcctgcagggttgcgatagtaattgaaccactggatattactaaaatctcatttcataatgacctgattattattgtgaaatctca
tttcataatgacctgattatcataatgactggatattatttttgtggtgcatgatttagtggatattagtgaaatctcatttcatattgacctgattattattgtttcttcatttggaaaatagGAGGGCAGAGGCACTGCTAGTATCGGCTCTACAACTTCACTTCCACGGGGAACGCCGACCCG

Intron 2 GCCAACCTGT ... TGCCGCAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.88	49	28	37
A2	33.33	16	6	10
A3	44.58	23	17	20
A4	39.62	16	9	12
A5	31.82	15	5	9
A6	66.67	1	2	2
A7	54.55	5	5	7
A8	42.86	6	4	5
A9	38.46	4	3	2
A10	42.86	2	1	2

>TRINITY_DN136114_c5_g1_i11 from sample A1 / Example spliced isoform

GATGAGTCGTCATTGGGGTCAGGATGAGTTATCCGTAAGCCATCTCAGGATGAGTCGTCATTGGGGTCAGGATGAGTTATCCGTAAGCCATCTCAGGATGAGTCGTCATTGGGGTCAGGCAGAGTTAATCTCCAACCCCACAATTCCGGTGTTCGGTTCTTGAAGTAGTATGCGATTGCTGAGCATCTTGAATCATCAAA
GAGCCCTTTAATAGTTTCCTTCCACTCTCTACACCGCTGCAGCCACGGGCGTCGAAAGCGATGGATCAACTCTTTGGCATTTCCAACTTAATGTTGCATCCTCTGCGGATCTTGGAATGTAGTTGGAATCTCAAGCAAGCCGCCCTTTATACGATATATTTCTTATTTTTTAATTAAAAAATGGGCAT

>TRINITY_DN136114_c5_g1_i15 from sample A1 / Example unspliced isoform:

GATGAGTCGTCATTGGGGTCAGGATGAGTTATCCGTAAGCCATCTCAGGATGAGTCGTCATTGGGGTCAGGATGAGTTATCCGTAAGCCATCTCAGGATGAGTCGTCATTGGGGTCAGGCAGAGTTAATCTCCAACCCCACAATTCCGGTGTTCGGTTCTTGAAGTAGTATgccaacctgttctcttgccgcagagGCGA
TTGCTGAGCATCTTGAATCATCAAAGAGCCCTTTAATAGTTTCCTTCCACTCTCTACACCGCTGCAGCCACGGGCGTCGAGAGCGATGGATCAACTCTTTGGCATTTCCAACTTAATGTTGCATCCTCTGCGGATCTTGGAATGTAGTTGGAATCTC

Intron 3 GTAAACCTGC ... CTTCCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.65	105	14	31
A2	23.08	55	13	20
A3	15.42	85	11	20
A4	19.81	83	14	27
A5	11.93	48	4	9
A6	8.48	178	7	26
A7	11.06	189	19	28
A8	7.05	112	7	10
A9	9.09	185	14	23
A10	9.29	244	19	31

>TRINITY_DN104910_c0_g1_i8 from sample A4 / Example spliced isoform

CGGAAAAGCAAAAAGAGAAGGTGGTTATAGATTTGCTTAGATAGCGCAGGCCTTCAAAATATTGAGTATCAATTTGTAGTGTGCTGTGTGTCTTTCTTGAAGAGGAGGGTGGCCGCATATTCTCCATCTGGGTTGGAGTGGAACCAAAGTTGTTTTTCTACTCCAAGATATGTCACACAGCAGAGAATATCGTCAGAGCC
ACAGTTGAACCCATGTGAAGGTCGACGTAGGATTTGCTCCCGGAATTCTCAGGCTTCACTTCCACGATTGCATTGTTCAGGGTTGCGATAGTAATTGAACCACTGGATATTAATGAAATCTCATTTC

>TRINITY_DN104910_c0_g1_i10 from sample A4 / Example unspliced isoform:

TAGGACTTGCTCCCGGAATTCTCAGGCTTCACTTCCACGATTGCATTGTTCAGgtaaacctgcagtttttagttgctgaggataattgcttctgctgcttcttcttcctgcagGGTTGCGATAGTAATTGAACCACTGGATATTAATGAAATCTCATTTCTACTGGTATCGGCTCTACAACTTCACTTCCACGGGGAACG
CCGACCCGACAATAAACTCGGCGAATTTCGATGCCTCGTTTTTCAAGAATATAAGAGACGGGAATGGCGTTCTGGAGTCCGA

Intron 4 GTAAATTCAT ... TGGAAAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	95.17	7	50	138
A2	94.83	6	30	110
A3	95.92	4	28	94
A4	91.14	7	53	72
A5	87.14	9	19	61
A6	100	0	43	200
A7	97.67	7	39	293
A8	95.85	8	31	185
A9	99.67	1	46	303
A10	97.14	9	45	306

>TRINITY_DN136114_c5_g1_i2 from sample A1 / Example spliced isoform

CTGGCTCCTTCCACGATTGCATTGTTCAGGAGGGCAGAGGCACTGCGAGTTCTTGAGGTATCGGCTCTACAATAAACTCGGTGAATCTACCTTCACTTCCAGTCGCGCTCGATAAAGGAAGCGAAACCAATTTCGATGCCTCGTTTTTCAAGAATATAAGAGACGGGAATGGCGTTCTGGAGTCCGACCAGAGACTTTGG
TCGGATGAGTCTGTGGTCATCTGTT

>TRINITY_DN136114_c5_g1_i3 from sample A1 / Example unspliced isoform:

GGAGCTGTGAAGAGGAGCTGTCACACAGCAGAGAATATCGTCAGATCCACAGTTGAAGCCCATGTGAAGGTCGACGTAGGACTTGCTCCCGGAATTCTCAGGCTTCACTTCCACGATTGCATTGTTCAGgtaaattcatgcgaactattcttttaaacctgcagtttttagtggtgcatgattcccagtcattcccggat
tagttgctgaagataattgcttctgctgcttcttcttcctgcagggttgcgatagtaattgaaccactggatattaatgaaatctcatttcatattgacctgattattattgtttcttcatttggaaaatagGAGGGCAGAGGCACTGCGAGTTCTTGAGGTATCGGCTCTACAACTTCACTTCCACAGGGAACGCCGAC
CCGACAATAAACTCGGCGAATCTGCCTTCACTTCCAGTCCGCGCTCGATAAAGGAAGCGAAACCAATTTCGATGCCTCGTTTTTCAAGAATATAAGAGACGGGAATGGCGTTCTGGAGTCCGACCAGAGACTTTGGTCGGATGAGTCTGTGGTCATCTGTTTGGGCATCGGCTCTGGAAGGACCTCTAATGG

Intron 5 GTAATTGAAC ... TGGAAAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.99	49	73.5	20
A2	53.57	39	45	14
A3	50.56	44	45	14
A4	34.72	47	25	7
A5	63.97	20	35.5	4
A6	49.22	130	126	32
A7	47.03	107	95	32
A8	37.19	125	74	38
A9	43.93	157	123	38
A10	42.08	106	77	30

>TRINITY_DN43464_c2_g1_i3 from sample A8 / Example spliced isoform

CCGGAAACGCAAGAAAGAGATGGTGGTTATAGATTTGCTTAGATTGGTCCATAAGATAAATCTGGCCTTTCAAATTTAAAATGAAAGCCGTTAGAAAAGAAAGAGAGAAGTGGAAGCCCGGAAAAGCAAGAAAGAGAGAAGTGGAAGACCAGAAAAGCAAGAAAGAGAAGGTAGTTATAGATTTGCTTAGATAGCACAGG
CATTCAAAATATTGAGTATCGATTTGTAGTGTGCTGTGTGTCTTTCTTGAAGAGGAGGGTGGCCGCATATTCTCCATCTGTGTTGGAGTGGAAGCAAAGGGAACCAAAGTTGTTTTCTACTACCAAGATCTGTCACACAGCAGAGAATATCGTCAGATCCACAGTTGAAGCCCATGTGAAGGTCGGCGTAGGACTTGCTC
CCGGAATTTGTTTTCATGCGAACTATTCTTTGAAACCTGCAGTTTTTAGTGGTCCATGATTCCCAGTCATTCCCGGATTAGTTGCTGAAGATAATTGCTTCTGCTGCTTCTTCTTCCTGCAGGGTTGCGATAGAGGGCAGAGGCACTGCTAGTATCGGCTCTACAACTTCACTTCCACGGGGAACGCCGACCCGACAATA
AACTCGGCGAATCTGCCTTCACTTCCAGTCGCGCTCGATAAAGGAAGCGAAACCAATTTAGATGCCTCGTTTTTCAAGAATATAAGAGACGGGAATGGTGTTCTGGAGTCCCACCAGAGACTTTGGTCGTATGAGTCTGTGGTCATCTGTTTGGGCAGCTGCTCTGGAAGGACCTCTAATGGGCATCTGCTTGGCCATTC
CGGTCAATACTG

>TRINITY_DN43464_c2_g1_i9 from sample A8 / Example unspliced isoform:

CCGGAAACGCAAGAAAGAGATGGTGGTTATAGATTTGCTTAGATTGGTCCATAAGATAAATCTGGCCTTTCAAATTTAAAATGAAAGCCGTTAGAAAAGAAAGAGAGAAGTGGAAGCCCGGAAAAGCAAGAAAGAGAGAAGTGGAAGACCAGAAAAGCAAGAAAGAGAAGGTAGTTATAGATTTGCTTAGATAGCACAGG
CATTCAAAATATTGAGTATCGATTTGTAGTGTGCTGTGTGTCTTTCTTGAAGAGGAGGGTGGCCGCATATTCTCCATCTGTGTTGGAGTGGAAGCAAAGGGAACCAAAGTTGTTTTCTACTACCAAGATCTGTCACACAGCAGAGAATATCGTCAGATCCACAGTTGAAGCCCATGTGAAGGTCGGCGTAGGACTTGCTC
CCGGAATTTGTTTTCATGCGAACTATTCTTTGAAACCTGCAGTTTTTAGTGGTCCATGATTCCCAGTCATTCCCGGATTAGTTGCTGAAGATAATTGCTTCTGCTGCTTCTTCTTCCTGCAGGGTTGCGATAgtaattgaaccactggatattaataaaatctcatttcataatgacctgattattattgtgaaatctca
tttcataatgacctgattatcataatgactggatattatttttgtggtgcatgatttagtggatattagtgaaatctcatttcatattgacctgattattattgtttcttcatttggaaaatagGAGGGCAGAGGCACTGCTAGTATCGGCTCTACAACTTCACTTCCACGGGGAACGCCGACCCG