NA ----> NA

Intron 1 GTATTGTACT ... TTTCACAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36	8	2	7
A2	33.33	5	1	4
A3	37.5	10	4	8
A4	33.33	6	3	3
A5	33.33	4	2	2
A6	36.84	54	18	45
A7	45.74	51	38	48
A8	41.9	52	30	45
A9	39.3	61	42	37
A10	35.59	57	29	34

>TRINITY_DN54186_c0_g1_i2 from sample A3 / Example spliced isoform

AAGAAAGTCGTGCAGGTCGGGAGAGAGGGAAAACTCGAACTCGAGACCTCTGAATAAATGAACAGCAGTTAAACTTTATAGCTCGGGCGGTCACACTTCAAACGGTCTGGGATGGAGCCGCTTACTCTCAGCGTAGGACAGTTGCGGAAGGCCAGGGAAGCCGCAGGGAATATCATGCAGACGAAGCAGCCAGCCGAGGC
GCTCACCATTTTCACAAAGGGTTTGAGGCCGGTTCGTTCCATGCGGGAGATGGAGTCTCTCTTACAGGAAGAGGCGCAACAGCTTCACGATATGCAAGACAAGTCGCACGCTATTCGCTTGTACTCTTCTTCAGGTTCTCGTCCCGTGGATATTGTGAAGGGAGCTTCTTCCGCTCCGTTTTAACTTTGCACCTCATGAG
ATTCGAACACGATACTTTGGTTCAGACTTAGTTAAATGCTTAACAATATTGACAACAATCGATGAAACTAACCGCTTGGACTGGGAATATTCACATCCCTATTCTTATGTGAAGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAG

>TRINITY_DN54186_c0_g1_i1 from sample A3 / Example unspliced isoform:

AAGAAAGTCGTGCAGGTCGGGAGAGAGGGAAAACTCGAACTCGAGACCTCTGAATAAATGAACAGCAGTTAAACTTTATAGCTCGGGCGGTCACACTTCAAACGGTCTGGGATGGAGCCGCTTACTCTCAGCGTAGGACAGTTGCGGAAGGCCAGGGAAGCCGCAGGGAATATCATGCAGACGAAGCAGCCAGCCGAGGC
GCTCACCATTTTCACAAAGgtattgtactctttattgagaaaaataggaagccgcggtggatatcatccagaagcagccagacgaggcactcaccattttcacaaagGGTTTGAGGCCGGTTCGTTCCATGCGGGAGATGGAGTCTCTCTTACAGGAAGAGGCGCAACAGCTTCACGATATGCAAGACAAGTCGCACGCT
ATTCGCTTGTACTCTTCTTCAGGTTCTCGTCCCGTGGATATTGTGAAGGGAGCTTCTTCCGCTCCGTTTTAACTTTGCACCTCATGAGATTCGAACACGATACTTTGGTTCAGACTTAGTTAAATGCTTAACAATATTGACAACAATCGATGAAACTAACCGCTTGGACTGGGAATATTCACATCCCTATTCTTATGTGA
AGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAG

Intron 2 GTATTCTTTA ... TTTACTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	6.67	7	1	0
A2	20	4	0	2
A3	11.11	8	1	1
A4	25	3	0	2
A5	0	2	0	0
A6	15.09	45	3	13
A7	19.33	48	7	16
A8	20.35	45	8	15
A9	11.9	37	3	7
A10	13.92	34	0	11

>TRINITY_DN35393_c0_g1_i6 from sample A8 / Example spliced isoform

CCCCGGACCGATCAAAGAAAGTCGTGCAGGTCGGGAGAGAGGGAAAACTCGAACTCGAGACCTCTGAATAAATGAACAGCAGTTAAACTTTATAGCTCGGGCGGTCACACTTCAAACGGTCTGGGATGGAGCCGCTTACTCTCAGCGTAGGACAGTTGCGGAAGGCCAGGGAAGCCGCAGGGAATATCATGCAGACGAAG
CAGCCAGCCGAGGCGCTCACCATTTTCACAAAGGTATTGTACTCTTTATTGAGAAAAATAGGAAGCCGCGGTGGATATCATCCAGAAGCAGCCAGACGAGGCACTCACCATTTTCACAAAGGGTTTGAGGCCGGTTCGTTCCATGCGGGAGATGGAGTCTCTCTTACAGGAAGAGGCGCAACAGCTTCACGATATGCAAG
ACAAGTCGCACGCTATTCGCTTGTACTCTTCTTCAGGTTCTCGTCCCGTGGATATTGTGAAGGGAGCTTCTTCCGCTCCGTTTTAACTTTGCACCTCATGAGATTCGAACACGATACTTTGGTTCAGACTTAGTTAAATGCTTAACAATATTGACAACAATCGATGAAACTAACCGCTTGGACTGGGAATATTCACATCC
CTATTCTTATGTGAAGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAGTTAGTGAAGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAGTTAGATTGTGGAGTTTATTTATGTTATTATGGAGGAGAAGCTTTAACACATAGATCCATCTCTTAAACTTGTATCTATCAAGC
ATCCAGAAGGATCCAATTTCTTGAGGTGTATTTTTTTTTTAAAATTTAAACTCAAGAAATTTGATATCTCTTGATTGATAGACTCGGGTTTAAGTTTTAAGTTTTTATTTTATAAA

>TRINITY_DN35393_c0_g1_i5 from sample A8 / Example unspliced isoform:

CCCCGGACCGATCAAAGAAAGTCGTGCAGGTCGGGAGAGAGGGAAAACTCGAACTCGAGACCTCTGAATAAATGAACAGCAGTTAAACTTTATAGCTCGGGCGGTCACACTTCAAACGGTCTGGGATGGAGCCGCTTACTCTCAGCGTAGGACAGTTGCGGAAGGCCAGGGAAGCCGCAGGGAATATCATGCAGACGAAG
CAGCCAGCCGAGGCGCTCACCATTTTCACAAAGGTATTGTACTCTTTATTGAGAAAAATAGGAAGCCGCGGTGGATATCATCCAGAAGCAGCCAGACGAGGCACTCACCATTTTCACAAAGgtattctttactctttattgagaaaatattgccgggatttaaatgtgaaatcctttaatttctttactgtagGGTTTGA
GGCCGGTTCGTTCCATGCGGGAGATGGAGTCTCTCTTACAGGAAGAGGCGCAACAGCTTCACGATATGCAAGACAAGTCGCACGCTATTCGCTTGTACTCTTCTTCAGGTTCTCGTCCCGTGGATATTGTGAAGGGAGCTTCTTCCGCTCCGTTTTAACTTTGCACCTCATGAGATTCGAACACGATACTTTGGTTCAGA
CTTAGTTAAATGCTTAACAATATTGACAACAATCGATGAAACTAACCGCTTGGACTGGGAATATTCACATCCCTATTCTTATGTGAAGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAGTTAGTGAAGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAGTTAGATTGTGG
AGTTTATTTATGTTATTATGGAGGAGAAGCTTTAACACATAGATCCATCTCTTAAACTTGTATCTATCAAGCATCCAGAAGGATCCAATTTCTTGAGGTGTATTTTTTTTTTAAAATTTAAACTCAAGAAATTTGATATCTCTTGATTGATAGACTCGGGTTTAAGTTTTAAGTTTTTATTTTATAAA

Intron 3 GTATTGTACT ... TTTACTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.11	8	2	0
A2	23.08	5	1	2
A3	20	10	4	1
A4	29.41	6	3	2
A5	20	4	2	0
A6	19.4	54	13	13
A7	33.77	51	36	16
A8	29.73	52	29	15
A9	27.38	61	39	7
A10	25.49	57	28	11

>TRINITY_DN35393_c0_g1_i3 from sample A8 / Example spliced isoform

CCCCGGACCGATCAAAGAAAGTCGTGCAGGTCGGGAGAGAGGGAAAACTCGAACTCGAGACCTCTGAATAAATGAACAGCAGTTAAACTTTATAGCTCGGGCGGTCACACTTCAAACGGTCTGGGATGGAGCCGCTTACTCTCAGCGTAGGACAGTTGCGGAAGGCCAGGGAAGCCGCAGGGAATATCATGCAGACGAAG
CAGCCAGCCGAGGCGCTCACCATTTTCACAAAGGGTTTGAGGCCGGTTCGTTCCATGCGGGAGATGGAGTCTCTCTTACAGGAAGAGGCGCAACAGCTTCACGATATGCAAGACAAGTCGCACGCTATTCGCTTGTACTCTTCTTCAGGTTCTCGTCCCGTGGATATTGTGAAGGGAGCTTCTTCCGCTCCGTTTTAACT
TTGCACCTCATGAGATTCGAACACGATACTTTGGTTCAGACTTAGTTAAATGCTTAACAATATTGACAACAATCGATGAAACTAACCGCTTGGACTGGGAATATTCACATCCCTATTCTTATGTGAAGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAGTTAGTGAAGGAGCTCAAAATGAATGG
AAAAATGCAAAATGTATTAATATGATGAGTTAGATTGTGGAGTTTATTTATGTTATTATGGAGGAGAAGCTTTAACACATAGATCCATCTCTTAAACTTGTATCTATCAAGCATCCAGAAGGATCCAATTTCTTGAGGTGTATTTTTTTTTTAAAATTTAAACTCAAGAAATTTGATATCTCTTGATTGATAGACTCGGG
TTTAAGTTTTAAGTTTTTATTTTATAAA

>TRINITY_DN35393_c0_g1_i5 from sample A8 / Example unspliced isoform:

CCCCGGACCGATCAAAGAAAGTCGTGCAGGTCGGGAGAGAGGGAAAACTCGAACTCGAGACCTCTGAATAAATGAACAGCAGTTAAACTTTATAGCTCGGGCGGTCACACTTCAAACGGTCTGGGATGGAGCCGCTTACTCTCAGCGTAGGACAGTTGCGGAAGGCCAGGGAAGCCGCAGGGAATATCATGCAGACGAAG
CAGCCAGCCGAGGCGCTCACCATTTTCACAAAGgtattgtactctttattgagaaaaataggaagccgcggtggatatcatccagaagcagccagacgaggcactcaccattttcacaaaggtattctttactctttattgagaaaatattgccgggatttaaatgtgaaatcctttaatttctttactgtagGGTTTGA
GGCCGGTTCGTTCCATGCGGGAGATGGAGTCTCTCTTACAGGAAGAGGCGCAACAGCTTCACGATATGCAAGACAAGTCGCACGCTATTCGCTTGTACTCTTCTTCAGGTTCTCGTCCCGTGGATATTGTGAAGGGAGCTTCTTCCGCTCCGTTTTAACTTTGCACCTCATGAGATTCGAACACGATACTTTGGTTCAGA
CTTAGTTAAATGCTTAACAATATTGACAACAATCGATGAAACTAACCGCTTGGACTGGGAATATTCACATCCCTATTCTTATGTGAAGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAGTTAGTGAAGGAGCTCAAAATGAATGGAAAAATGCAAAATGTATTAATATGATGAGTTAGATTGTGG
AGTTTATTTATGTTATTATGGAGGAGAAGCTTTAACACATAGATCCATCTCTTAAACTTGTATCTATCAAGCATCCAGAAGGATCCAATTTCTTGAGGTGTATTTTTTTTTTAAAATTTAAACTCAAGAAATTTGATATCTCTTGATTGATAGACTCGGGTTTAAGTTTTAAGTTTTTATTTTATAAA