sp|O22704|CYP5F_ARATH ----> Cytochrome B5-like protein

Intron 1 GTATATGATA ... GAGCATCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.5	18	52	36
A2	71.55	20	50	47
A3	71.94	18	46	45
A4	66.34	15.5	32	24
A5	63.68	14	26	23
A6	68.18	83	172	174
A7	72.72	124	327	321
A8	71.58	116.5	291	280
A9	70.08	127	301	273
A10	70.8	97.5	239	219

>TRINITY_DN39111_c0_g1_i2 from sample A10 / Example spliced isoform

GTATTATCAATAAGCAGAATCGAACTGGCCAGAGTCATCAGCCAAAATGGTGAGCATGGAGATGGGGATAGCGGTTTCGTTACTCCTGGCGACTCTCCTAGCTGCATTTGTTTTCATCGGCAAAGGGTCTCGTAGATCTGCTACAGCCAAGGCCCCTGTCAACCATCTCACTACATGGGCAGGAGTTTACTGGACATAAC
CATAGGAAAACAGGGCCCCTCTTCTTGGTTCAGACCTCATGGTCCTAACATTTGATGGAAAATGCTCAGCTTTCAAGATTGTATTACAATCAGCTTCCATCCTTTCCTTTCTGGACTCAGAAGGCTTATAGTAAATCTGAAGTTGCTCAACACAACAAAAGGACTGACTGTTGGATCATAATAAAGGACAAGGTGGTGAT
GCAATACTAGCTCATGCTGGGGATGACTCTACTGAAGGGTTTTACGGACCACAACATGCTTCAAGGGTCTTTGATATGGTGGATGAATTTTATGTGGGAGACTTGAAGCACTAAATACAAATAAAAACTTTCGTCACCATCTTTAGCTTTTATCAACAGGAAAGTCCCATCAATCATATCAGATCAAATAGACAGGCTAA
TGGAGTCAGTAAGATTGTGTGCTTGGTTTAAAAGTTGGAATGCTTGTGTTGTAGGTGAAAGAGTGACAAGCTTTCAATTCTAGAATGATTGAAAACTTTTTCCACTTCTGAAAATGGAAATATGCAGTGTGACTTTCTGATAAGGATAAAATAACAAAAGTGCTAGAGAAAAACAAATTACAAGTCAGGATTTGTTACAA
AAGTTTTTTTTACCTTTTGACACTGCAGTTAGGAGATGTGAAAATCGGATTATTCAGAAGGATTGTCCTAGAGGGAATTCCTTGTTGTCTTAAAATTCATGTTACAACACCATAGCTATGTGTAAGACTTTGCGAGCAATTAATTTTCCTCAATATAGCCATATTTTATCATTATTAAGGCCCTCTTCTCCATTTTGGTG
CCATAGATATGATTCCAAATCTACACCTTACATACGACT

>TRINITY_DN39111_c0_g1_i1 from sample A10 / Example unspliced isoform:

GTATTATCAATAAGCAGAATCGAACTGGCCAGAGTCATCAGCCAAAATGGTGAGCATGGAGATGGGGATAGCGGTTTCGTTACTCCTGGCGACTCTCCTAGCTGCATTTGTTTTCATCGGCAAAGGGTCTCGTAGATCTGCTACAGCCAAGGCCCCTGTCAACCATCTCACTACTCAGAAGGCTTATAGTAAATCTGAAG
TTGCTCAACACAACAAAAGGACTGACTGTTGGATCATAATAAAGGACAAGgtatatgatatgacttcctatgtagatgagcatccagGTGGTGATGCAATACTAGCTCATGCTGGGGATGACTCTACTGAAGGGTTTTACGGACCACAACATGCTTCAAGGGTCTTTGATATGGTGGATGAATTTTATGTGGGAGACTTG
AAGCACTAAATACAAATAAAAACTTTCGTCACCATCTTTAGCTTTTATCAACAGGAAAGTCCCATCAATCATATCAGATCAAATAGACAGGCTAATGGAGTCAGTAAGATTGTGTGCTTGGTTTAAAAGTTGGAATGCTTGTGTTGTAGGTGAAAGAGTGACAAGCTTTCAATTCTAGAATGATTGAAAACTTTTTCCAC
TTCTGAAAATGGAAATATGCAGTGTGACTTTCTGATAAGGATAAAATAACAAAAGTGCTAGAGAAAAACAAATTACAAGTCAGGATTTGTTACAAAAGTTTTTTTTACCTTTTGACACTGCAGTTAGGAGATGTGAAAATCGGATTATTCAGAAGGATTGTCCTAGAGGGAATTCCTTGTTGTCTTAAAATTCATGTTAC
AACACCATAGCTATGTGTAAGACTTTGCGAGCAATTAATTTTCCTCAATATAGCCATATTTTATCATTATTAAGGCCCTCTTCTCCATTTTGGTGCCATAGATATGATTCCAAATCTACACCTTACATACGACT

Intron 2 GTATATGATA ... TCCTATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.6	48.8	42.9	37.5
A2	46.12	52.6	42.5	46.5
A3	44.99	50.6	41.5	41
A4	42.97	32.4	24.5	23.5
A5	44.55	29.6	24	23.5
A6	45.66	174.6	145.5	146
A7	47.21	334.6	291	305
A8	45.92	305.4	251	265
A9	45.13	319.4	257	264.5
A10	45.65	246.2	204	208

>TRINITY_DN109080_c0_g1_i3 from sample A1 / Example spliced isoform

AAGTATTATCAATAAGCAGAATCGAACTGGCCAGAGTCATCAGCCAAAATGGTGAGCATGGAGATGGGGATAGCGGTTTCGTTACTGCTGGCGACTCTCCTAGCTGCATTTGTTTTCATCGGCAAAGGGTCTCGTAGATCTGCTACAGCCAAGGCCCCTGTCAACCATCTCACTACTCAGAAGGCTTATAGTAAATCTGA
AGTTGCTCAACACAACAAAAGGACTGACTGTTGGATCATAATAAAGGACAAGATGAGCATCCAGGTGGTGATGCAATACTAGCTCATGCTGGGGATGACTCTACTGAAGGGTTTTACGGACCACAACATGCTTCAAGGGTCTTTGATATGGTGGATGAATTTTATGTGGGAGACTTGAAGCACTAAATACAAATAAAAAC
TTTCGTCACCATCTTTAGCTTTTATCAACAGGAAAGTCCCATCAATCATATCAGATCAAATAGACAGGCTAATGGAGTCAGTAAGATTGTGTGCTTGGTTTAAAAGTTGGAATGCTTGTGTTGTAGGTGAAAGAGTGACAAGCTTTCAATTCTAGAATGATTGAAAACTTTTTCCACTTCTGAAAATGGAAATATGCAGT
GTGACTTTCTGATAAGGATAAAATAACAAAAGTGCTAGAGAAAAACAAATTACAAGTCAGGATTTGTTACAAAAGTTTTTTTTACCTTTTGACACTGCAGTTAGGAGATGTGAAAATCGGATTATTCAGAAGGATTGTCCTAGAGGGAATTCCTTGTTGTCTTAAAATTCATGTTACAACACCATAGCTATGTGTAAGAC
TTTGCGAGCAATTAATTTTCCTCAATATAGCCATATTTTATCATTATTAAGGCCCTCTTCTCCATTTTGG

>TRINITY_DN109080_c0_g1_i1 from sample A1 / Example unspliced isoform:

AAGTATTATCAATAAGCAGAATCGAACTGGCCAGAGTCATCAGCCAAAATGGTGAGCATGGAGATGGGGATAGCGGTTTCGTTACTGCTGGCGACTCTCCTAGCTGCATTTGTTTTCATCGGCAAAGGGTCTCGTAGATCTGCTACAGCCAAGGCCCCTGTCAACCATCTCACTACTCAGAAGGCTTATAGTAAATCTGA
AGTTGCTCAACACAACAAAAGGACTGACTGTTGGATCATAATAAAGGACAAGgtatatgatatgacttcctatgtagATGAGCATCCAGGTGGTGATGCAATACTAGCTCATGCTGGGGATGACTCTACTGAAGGGTTTTACGGACCACAACATGCTTCAAGGGTCTTTGATATGGTGGATGAATTTTATGTGGGAGACT
TGAAGCACTAAATACAAATAAAAACTTTCGTCACCATCTTTAGCTTTTATCAACAGGAAAGTCCCATCAATCATATCAGATCAAATAGACAGGCTAATGGAGTCAGTAAGATTGTGTGCTTGGTTTAAAAGTTGGAATGCTTGTGTTGTAGGTGAAAGAGTGACAAGCTTTCAATTCTAGAATGATTGAAAACTTTTTCC
ACTTCTGAAAATGGAAATATGCAGTGTGACTTTCTGATAAGGATAAAATAACAAAAGTGCTAGAGAAAAACAAATTACAAGTCAGGATTTGTTACAAAAGTTTTTTTTACCTTTTGACACTGCAGTTAGGAGATGTGAAAATCGGATTATTCAGAAGGATTGTCCTAGAGGGAATTCCTTGTTGTCTTAAAATTCATGTT
ACAACACCATAGCTATGTGTAAGACTTTGCGAGCAATTAATTTTCCTCAATATAGCCATATTTTATCATTATTAAGGCCCTCTTCTCCATTTTGG

Intron 3 ATGGGCAGGA ... CTTTCTGGAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.6	98.8	39.5	17.4
A2	15.91	89.4	16.9	10.5
A3	31.15	65.3	29.5	12.8
A4	24.57	56.7	18.5	4.7
A5	19.56	37.1	9	9.8
A6	26.32	280.2	100.1	24.8
A7	27	469	173.4	76.4
A8	29.31	395	163.7	52.3
A9	27.48	495.7	187.8	73.6
A10	26.98	387.8	143.3	45.7

>TRINITY_DN109080_c0_g1_i3 from sample A1 / Example spliced isoform

AAGTATTATCAATAAGCAGAATCGAACTGGCCAGAGTCATCAGCCAAAATGGTGAGCATGGAGATGGGGATAGCGGTTTCGTTACTGCTGGCGACTCTCCTAGCTGCATTTGTTTTCATCGGCAAAGGGTCTCGTAGATCTGCTACAGCCAAGGCCCCTGTCAACCATCTCACTACTCAGAAGGCTTATAGTAAATCTGA
AGTTGCTCAACACAACAAAAGGACTGACTGTTGGATCATAATAAAGGACAAGATGAGCATCCAGGTGGTGATGCAATACTAGCTCATGCTGGGGATGACTCTACTGAAGGGTTTTACGGACCACAACATGCTTCAAGGGTCTTTGATATGGTGGATGAATTTTATGTGGGAGACTTGAAGCACTAAATACAAATAAAAAC
TTTCGTCACCATCTTTAGCTTTTATCAACAGGAAAGTCCCATCAATCATATCAGATCAAATAGACAGGCTAATGGAGTCAGTAAGATTGTGTGCTTGGTTTAAAAGTTGGAATGCTTGTGTTGTAGGTGAAAGAGTGACAAGCTTTCAATTCTAGAATGATTGAAAACTTTTTCCACTTCTGAAAATGGAAATATGCAGT
GTGACTTTCTGATAAGGATAAAATAACAAAAGTGCTAGAGAAAAACAAATTACAAGTCAGGATTTGTTACAAAAGTTTTTTTTACCTTTTGACACTGCAGTTAGGAGATGTGAAAATCGGATTATTCAGAAGGATTGTCCTAGAGGGAATTCCTTGTTGTCTTAAAATTCATGTTACAACACCATAGCTATGTGTAAGAC
TTTGCGAGCAATTAATTTTCCTCAATATAGCCATATTTTATCATTATTAAGGCCCTCTTCTCCATTTTGG

>TRINITY_DN109080_c0_g1_i2 from sample A1 / Example unspliced isoform:

AAGTATTATCAATAAGCAGAATCGAACTGGCCAGAGTCATCAGCCAAAATGGTGAGCATGGAGATGGGGATAGCGGTTTCGTTACTGCTGGCGACTCTCCTAGCTGCATTTGTTTTCATCGGCAAAGGGTCTCGTAGATCTGCTACAGCCAAGGCCCCTGTCAACCATCTCACTACatgggcaggagtttactggacata
accataggaaaacagggcccctcttcttggttcagacctcatggtcctaacatttgatggaaaatgctcagctttcaagattgtattacaatcagcttccatcctttcctttctggacTCAGAAGGCTTATAGTAAATCTGAAGTTGCTCAACACAACAAAAGGACTGACTGTTGGATCATAATAAAGGACAAGGTATAT
GATATGACTTCCTATGTAGATGAGCATCCAGGTGGTGATGCAATACTAGCTCATGCTGGGGATGACTCTACTGAAGGGTTTTACGGACCACAACATGCTTCAAGGGTCTTTGATATGGTGGATGAATTTTATGTGGGAGACTTGAAGCACTAAATACAAATAAAAACTTTCGTCACCATCTTTAGCTTTTATCAACAGGA
AAGTCCCATCAATCATATCAGATCAAATAGACAGGCTAATGGAGTCAGTAAGATTGTGTGCTTGGTTTAAAAGTTGGAATGCTTGTGTTGTAGGTGAAAGAGTGACAAGCTTTCAATTCTAGAATGATTGAAAACTTTTTCCACTTCTGAAAATGGAAATATGCAGTGTGACTTTCTGATAAGGATAAAATAACAAAAGT
GCTAGAGAAAAACAAATTACAAGTCAGGATTTGTTACAAAAGTTTTTTTTACCTTTTGACACTGCAGTTAGGAGATGTGAAAATCGGATTATTCAGAAGGATTGTCCTAGAGGGAATTCCTTGTTGTCTTAAAATTCATGTTACAACACCATAGCTATGTGTAAGACTTTGCGAGCAATTAATTTTCCTCAATATAGCCA
TATTTTATCATTATTAAGGCCCTCTTCTCCATTTTGG