sp|F4IG73|BCHC2_ARATH ----> BEACH domain-containing protein C2

Intron 1 GTACGTTCTG ... TTTCATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60	2	3	0
A2	71.43	2	5	0
A3	63.64	4	7	2
A4	28.57	5	2	0
A5	16.67	5	1	0
A6	9.09	10	1	0
A7	22.22	7	2	0
A8	25	9	3	0
A9	13.89	31	5	0
A10	0	4	0	0

>TRINITY_DN89328_c0_g2_i1 from sample A3 / Example spliced isoform

GGATAGACCTGATTTTTGGTTACAAGCAGCGTGGAATGGCTGCTTTGGCGGCAGATAACTTATTTCACTTTCTATCATATGAGGGTGCAGTTGATTTGGAGAAGATAGATGATCCCATTGAACGTGCTGGCCTTGAAGTTCAGATTCATGAATTTGGACAAGTGCCACAACAACTTTTTCAGAAATCTCACCGTACACGG
TTTCCATGTAAGACAAAAATTGGAGCTGCTTCATTGTCATCAATGCAAATTGATTTGGGAGCATTGATATATCAACTGATAACAGTAGCTTCATCCATAGAAAGTGACATTTCTTTGGAAGGCGAGAAGACAAGAGCAGCAACTAGTCTTGCTTCTGAATCTGAACAATTATTACACTTAAAGGATTTGCCATATCCACT
CCCGTCTATATACAATTGCAAAGAGAATCCTAAAATTGGTAGTCTTAAAGGGGCTATAGATGAAAGTGCAAATATGGAGAGCTGCAATGTGCAGAACAGAATGATAAGAATTGCTAATGATGATTATGATAGTATTTTGGTAACAAAATTATCCTTGGATGATGACGCTAAACTACAAATTAGGGATGTGGATGGTAAGA
ACTTAGAGAATGAGGAAAATGCTAAGTCCGATGAAACTACAGGAATGCCTTTGAATGGAGACCAAGGAAGAGTAGAGAATGAAATCAGGAGAAATGGTGATGAATCAAAAGATACTGTGCAGGATGATTGTGCTTGGTATTATAGGTTACGGCAACGTTTTTCAGAGCCACAATCTCTAAAGATGCATAGAGGAGCTGTT
TGTGCAGTCATCCTTTCTGAGGATAATGCATCAGAGTGCCTTACAATGTTTTCTGTTGGCAGAGATGGTTTTATCAAGGTTTACTCTATTACAGATGGGATTCAATTGCGTTCTACCAAACTTGGAAGCTTACCACTCTCTTCTCTAGCATTAGCTAAGAGCATGGATGCATATCCTATTGTGCTTGCAGGATCATTTGA
TAATTGCGTTTATGTGTACTCAGTAGATTATGGTCGTGCTCTTGCAAAGGTCAGAGTCCATGAAGATACTGTTTCATGTCTGCAAGTAATGGAATCTTCTTGCCAACAAATGGTCACAGCATCTTGGGATGCAACTGTCAAAATTTGGACTATGGATGAGGGAAGGGGTTCTTGGGCCACAACTTTAGGCTCATCTAAGA
ACACCAGAAATGCTAGTGTGGAATTTGTTGAGCATGATGGAGCTGTACTTTGTTTGGACATAGGATATCAAAATAACATTCTTGTATCAGGGTCCGAAGATGGATCAATCATAGCCTGGGATCCAAGAACTCCAAAGTCAATTTGGCATCAAGCACATAGAAATAAAGGCTCTCCAGCT

>TRINITY_DN89328_c0_g2_i2 from sample A3 / Example unspliced isoform:

GGATAGACCTGATTTTTGGTTACAAGCAGCGTGGAATGGCTGCTTTGGCGGCAGATAACTTATTTCACTTTCTATCATATGAGGGTGCAGTTGATTTGGAGAAGATAGATGATCCCATTGAACGTGCTGGCCTTGAAGTTCAGATTCATGAATTTGGACAAGTGCCACAACAACTTTTTCAGAAATCTCACCGTACACGG
TTTCCATGTAAGACAAAAATTGGAGCTGCTTCATTGTCATCAATGCAAATTGATTTGGGAGCATTGATATATCAACTGATAACAGTAGCTTCATCCATAGAAAGTGACATTTCTTTGGAAGGCGAGAAGACAAGAGCAGCAACTAGTCTTGCTTCTGAATCTGAACAATTATTACACTTAAAGGATTTGCCATATCCACT
CCCGTCTATATACAATTGCAAAGAGAATCCTAAAATTGGTAGTCTTAAAGGGGCTATAGATGAAAGTGCAAATATGGAGAGCTGCAATGTGCAGAACAGAATGATAAGAATTGCTAATGATGATTATGATAGTATTTTGGTAACAAAATTATCCTTGGATGATGACGCTAAACTACAAATTAGGGATGTGGATGGTAAGA
ACTTAGAGAATGAGGAAAATGCTAAGTCCGATGAAACTACAGGAATGCCTTTGAATGGAGACCAAGGAAGAGTAGAGAATGAAATCAGGAGAAATGGTGATGAATCAAAAGATACTGTGCAGGATGATTGTGCTTGGTATTATAGGTTACGGCAACGTTTTTCAGAGCCACAATCTCTAAAGATGCATAGAGGAGCTGTT
TGTGCAGTCATCCTTTCTGAGGATAATGCATCAGAGTGCCTTACAATGTTTTCTGTTGGCAGAGATGGTTTTATCAAGGTTTACTCTATTACAGATGGGATTCAATTGCGTTCTACCAAACTTGGAAGCTTACCACTCTCTTCTCTAGCATTAGCTAAGAGCATGGATGCATATCCTgtacgttctgtgttgcccctctg
aactgcatattaaaggttatcgcctctagctagaatgtttgttttctacgaattccatttacaaaagattattgaatatttttctttccatgttcaattactaatctgtattttcatttagATTGTGCTTGCAGG

Intron 2 GTTATCGCCT ... TTTCATTTAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	1	1	0
A2	33.33	6	3	0
A3	50	8	8	2
A4	0	1	0	0
A5	0	7	0	0
A6	0	6	0	0
A7	-1	0	0	0
A8	-1	0	0	0
A9	0	2	0	0
A10	0	2	0	0

>TRINITY_DN89328_c0_g2_i3 from sample A3 / Example spliced isoform

GGATAGACCTGATTTTTGGTTACAAGCAGCGTGGAATGGCTGCTTTGGCGGCAGATAACTTATTTCACTTTCTATCATATGAGGGTGCAGTTGATTTGGAGAAGATAGATGATCCCATTGAACGTGCTGGCCTTGAAGTTCAGATTCATGAATTTGGACAAGTGCCACAACAACTTTTTCAGAAATCTCACCGTACACGG
TTTCCATGTAAGACAAAAATTGGAGCTGCTTCATTGTCATCAATGCAAATTGATTTGGGAGCATTGATATATCAACTGATAACAGTAGCTTCATCCATAGAAAGTGACATTTCTTTGGAAGGCGAGAAGACAAGAGCAGCAACTAGTCTTGCTTCTGAATCTGAACAATTATTACACTTAAAGGATTTGCCATATCCACT
CCCGTCTATATACAATTGCAAAGAGAATCCTAAAATTGGTAGTCTTAAAGGGGCTATAGATGAAAGTGCAAATATGGAGAGCTGCAATGTGCAGAACAGAATGATAAGAATTGCTAATGATGATTATGATAGTATTTTGGTAACAAAATTATCCTTGGATGATGACGCTAAACTACAAATTAGGGATGTGGATGGTAAGA
ACTTAGAGAATGAGGAAAATGCTAAGTCCGATGAAACTACAGGAATGCCTTTGAATGGAGACCAAGGAAGAGTAGAGAATGAAATCAGGAGAAATGGTGATGAATCAAAAGATACTGTGCAGGATGATTGTGCTTGGTATTATAGGTTACGGCAACGTTTTTCAGAGCCACAATCTCTAAAGATGCATAGAGGAGCTGTT
TGTGCAGTCATCCTTTCTGAGGATAATGCATCAGAGTGCCTTACAATGTTTTCTGTTGGCAGAGATGGTTTTATCAAGGTTTACTCTATTACAGATGGGATTCAATTGCGTTCTACCAAACTTGGAAGCTTACCACTCTCTTCTCTAGCATTAGCTAAGAGCATGGATGCATATCCTGTACGTTCTGTGTTGCCCCTCTG
AACTGCATATTAAAGATTGTGCTTGCAGGATCATTTGATAATTGCGTTTATGTGTACTCAGTAGATTATGGTCGTGCTCTTGCAAAGGTCAGAGTCCATGAAGATACTGTTTCATGTCTGCAAGTAATGGAATCTTCTTGCCAACAAATGGTCACAGCATCTTGGGATGCAACTGTCAAAATTTGGACTATGGATGAGGG
AAGGGGTTCTTGGGCCACAACTTTAGGCTCATCTAAGAACACCAGAAATGCTAGTGTGGAATTTGTTGAGCATGATGGAGCTGTACTTTGTTTGGACATAGGATATCAAAATAACATTCTTGTATCAGGGTCCGAAGATGGATCAATCATAGCCTGGGATCCAAGAACTCCAAAGTCAATTTGGCATCAAGCACATAGAA
ATAAAGGCTCTCCAGCT

>TRINITY_DN89328_c0_g2_i2 from sample A3 / Example unspliced isoform:

GGATAGACCTGATTTTTGGTTACAAGCAGCGTGGAATGGCTGCTTTGGCGGCAGATAACTTATTTCACTTTCTATCATATGAGGGTGCAGTTGATTTGGAGAAGATAGATGATCCCATTGAACGTGCTGGCCTTGAAGTTCAGATTCATGAATTTGGACAAGTGCCACAACAACTTTTTCAGAAATCTCACCGTACACGG
TTTCCATGTAAGACAAAAATTGGAGCTGCTTCATTGTCATCAATGCAAATTGATTTGGGAGCATTGATATATCAACTGATAACAGTAGCTTCATCCATAGAAAGTGACATTTCTTTGGAAGGCGAGAAGACAAGAGCAGCAACTAGTCTTGCTTCTGAATCTGAACAATTATTACACTTAAAGGATTTGCCATATCCACT
CCCGTCTATATACAATTGCAAAGAGAATCCTAAAATTGGTAGTCTTAAAGGGGCTATAGATGAAAGTGCAAATATGGAGAGCTGCAATGTGCAGAACAGAATGATAAGAATTGCTAATGATGATTATGATAGTATTTTGGTAACAAAATTATCCTTGGATGATGACGCTAAACTACAAATTAGGGATGTGGATGGTAAGA
ACTTAGAGAATGAGGAAAATGCTAAGTCCGATGAAACTACAGGAATGCCTTTGAATGGAGACCAAGGAAGAGTAGAGAATGAAATCAGGAGAAATGGTGATGAATCAAAAGATACTGTGCAGGATGATTGTGCTTGGTATTATAGGTTACGGCAACGTTTTTCAGAGCCACAATCTCTAAAGATGCATAGAGGAGCTGTT
TGTGCAGTCATCCTTTCTGAGGATAATGCATCAGAGTGCCTTACAATGTTTTCTGTTGGCAGAGATGGTTTTATCAAGGTTTACTCTATTACAGATGGGATTCAATTGCGTTCTACCAAACTTGGAAGCTTACCACTCTCTTCTCTAGCATTAGCTAAGAGCATGGATGCATATCCTGTACGTTCTGTGTTGCCCCTCTG
AACTGCATATTAAAGgttatcgcctctagctagaatgtttgttttctacgaattccatttacaaaagattattgaatatttttctttccatgttcaattactaatctgtattttcatttagATTGTGCTTGCAGG

Intron 3 GTACGTTCTG ... CATATTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	2	3	1
A2	77.78	2	8	6
A3	66.67	4	8	8
A4	23.08	5	2	1
A5	52.38	5	4	7
A6	39.39	10	7	6
A7	12.5	7	2	0
A8	14.29	9	3	0
A9	10.14	31	5	2
A10	20	4	0	2

>TRINITY_DN89328_c0_g2_i1 from sample A3 / Example spliced isoform

GGATAGACCTGATTTTTGGTTACAAGCAGCGTGGAATGGCTGCTTTGGCGGCAGATAACTTATTTCACTTTCTATCATATGAGGGTGCAGTTGATTTGGAGAAGATAGATGATCCCATTGAACGTGCTGGCCTTGAAGTTCAGATTCATGAATTTGGACAAGTGCCACAACAACTTTTTCAGAAATCTCACCGTACACGG
TTTCCATGTAAGACAAAAATTGGAGCTGCTTCATTGTCATCAATGCAAATTGATTTGGGAGCATTGATATATCAACTGATAACAGTAGCTTCATCCATAGAAAGTGACATTTCTTTGGAAGGCGAGAAGACAAGAGCAGCAACTAGTCTTGCTTCTGAATCTGAACAATTATTACACTTAAAGGATTTGCCATATCCACT
CCCGTCTATATACAATTGCAAAGAGAATCCTAAAATTGGTAGTCTTAAAGGGGCTATAGATGAAAGTGCAAATATGGAGAGCTGCAATGTGCAGAACAGAATGATAAGAATTGCTAATGATGATTATGATAGTATTTTGGTAACAAAATTATCCTTGGATGATGACGCTAAACTACAAATTAGGGATGTGGATGGTAAGA
ACTTAGAGAATGAGGAAAATGCTAAGTCCGATGAAACTACAGGAATGCCTTTGAATGGAGACCAAGGAAGAGTAGAGAATGAAATCAGGAGAAATGGTGATGAATCAAAAGATACTGTGCAGGATGATTGTGCTTGGTATTATAGGTTACGGCAACGTTTTTCAGAGCCACAATCTCTAAAGATGCATAGAGGAGCTGTT
TGTGCAGTCATCCTTTCTGAGGATAATGCATCAGAGTGCCTTACAATGTTTTCTGTTGGCAGAGATGGTTTTATCAAGGTTTACTCTATTACAGATGGGATTCAATTGCGTTCTACCAAACTTGGAAGCTTACCACTCTCTTCTCTAGCATTAGCTAAGAGCATGGATGCATATCCTATTGTGCTTGCAGGATCATTTGA
TAATTGCGTTTATGTGTACTCAGTAGATTATGGTCGTGCTCTTGCAAAGGTCAGAGTCCATGAAGATACTGTTTCATGTCTGCAAGTAATGGAATCTTCTTGCCAACAAATGGTCACAGCATCTTGGGATGCAACTGTCAAAATTTGGACTATGGATGAGGGAAGGGGTTCTTGGGCCACAACTTTAGGCTCATCTAAGA
ACACCAGAAATGCTAGTGTGGAATTTGTTGAGCATGATGGAGCTGTACTTTGTTTGGACATAGGATATCAAAATAACATTCTTGTATCAGGGTCCGAAGATGGATCAATCATAGCCTGGGATCCAAGAACTCCAAAGTCAATTTGGCATCAAGCACATAGAAATAAAGGCTCTCCAGCT

>TRINITY_DN89328_c0_g2_i3 from sample A3 / Example unspliced isoform:

GGATAGACCTGATTTTTGGTTACAAGCAGCGTGGAATGGCTGCTTTGGCGGCAGATAACTTATTTCACTTTCTATCATATGAGGGTGCAGTTGATTTGGAGAAGATAGATGATCCCATTGAACGTGCTGGCCTTGAAGTTCAGATTCATGAATTTGGACAAGTGCCACAACAACTTTTTCAGAAATCTCACCGTACACGG
TTTCCATGTAAGACAAAAATTGGAGCTGCTTCATTGTCATCAATGCAAATTGATTTGGGAGCATTGATATATCAACTGATAACAGTAGCTTCATCCATAGAAAGTGACATTTCTTTGGAAGGCGAGAAGACAAGAGCAGCAACTAGTCTTGCTTCTGAATCTGAACAATTATTACACTTAAAGGATTTGCCATATCCACT
CCCGTCTATATACAATTGCAAAGAGAATCCTAAAATTGGTAGTCTTAAAGGGGCTATAGATGAAAGTGCAAATATGGAGAGCTGCAATGTGCAGAACAGAATGATAAGAATTGCTAATGATGATTATGATAGTATTTTGGTAACAAAATTATCCTTGGATGATGACGCTAAACTACAAATTAGGGATGTGGATGGTAAGA
ACTTAGAGAATGAGGAAAATGCTAAGTCCGATGAAACTACAGGAATGCCTTTGAATGGAGACCAAGGAAGAGTAGAGAATGAAATCAGGAGAAATGGTGATGAATCAAAAGATACTGTGCAGGATGATTGTGCTTGGTATTATAGGTTACGGCAACGTTTTTCAGAGCCACAATCTCTAAAGATGCATAGAGGAGCTGTT
TGTGCAGTCATCCTTTCTGAGGATAATGCATCAGAGTGCCTTACAATGTTTTCTGTTGGCAGAGATGGTTTTATCAAGGTTTACTCTATTACAGATGGGATTCAATTGCGTTCTACCAAACTTGGAAGCTTACCACTCTCTTCTCTAGCATTAGCTAAGAGCATGGATGCATATCCTgtacgttctgtgttgcccctctg
aactgcatattaaagATTGTGCTTGCAGGATCATTTGATAATTGCGTTTATGTGTACTCAGTAGATTATGGTCGTGCTCTTGCAAAGGTCAGAGTCCATGAAGATACTGTTTCATGTCTGCAAGTAATGGAATCTTCTTGCCAACAAATGGTCACAGCATCTTGGGATGCAACTGTCAAAATTTGGACTATGGATGAGGG
AAGGGGTTCTTGGGCCACAACTTTAGGCTCATCTAAGAACACCAGAAATGCTAGTGTGGAATTTGTTGAGCATGATGGAGCTGTACTTTGTTTGGACATAGGATATCAAAATAACATTCTTGTATCAGGGTCCGAAGATGGATCAATCATAGCCTGGGATCCAAGAACTCCAAAGTCAATTTGGCATCAAGCACATAGAA
ATAAAGGCTCTCCAGCT