NA ----> NA

Intron 1 GTATGAGCCT ... TTTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.62	34.4	5	15.2
A2	41.03	27	16.4	16
A3	38.51	48.9	10.1	22.6
A4	42.89	30.1	4.6	19.1
A5	36.59	20.8	6	11
A6	28.28	359.4	98.8	112.6
A7	30.17	202.3	55.2	62.8
A8	32.81	223.6	101.9	87.1
A9	33.58	166.9	52.9	69.8
A10	24.12	128.3	35.4	27.4

>TRINITY_DN115697_c11_g1_i4 from sample A1 / Example spliced isoform

GATCATTTTTAAAGTGCTTACATTGTCTGTGATAATGCAATTTTTACCTTTTGATTAAAAATTCATATCCATGTCTAAGAAGTTTAGAGATAAAACGGCTATGATATCATTGCAAACACCTTATTTTTAGCGCTGGCTATGAGTATTAGTGCAAGAACCTTATTTTTAGCCCTGGCGACACTGGCTACGAGTATCAGTGC
AAGAACCTTATTTCTAGCTATCATTGTGATTGAATACCTAATTTTGGCTGTGACGGTGGTTGAATTTTCACTTGTTTTGTCTGTAGGATAACATATGGATCTGGTTTGGACGAGGTCTGGCTTCTGCAAATCTTATTTATTTTACTATTAATCTCTTCTGTTTCCTCATTCCGAGATTCCTACCGCGTGCTTTCGAGAGA
TACTTTCAGGAAAGAGCTGAATGTAACGGAAAAGTATCCGAAATCGAACCTCAGAAGAAGGAGAAGGCGCAGAAGAAATCTGAATGATTCACATTGTATACAAATCTGAATATGATGCATTGTGTGTGCATGGTATGGCTTTTTAGCTTTAAAATACAGGACTTTGTTTTACATGCATAGAATCTACGGCCACTCCTTGG
TTTCGAACTTTCGAGGGACTCTAAAGCTGGGCAATGCTTGATTCTCGATTCCAATGGCGTGCAAACCCAAAATAAAATTCTCATATCTGAAAGAGAGCGTCTGTAATCTTGGCTATATAGTCATAAATCAATTAAGGTTTTTCGATGGATTTATGCTATTTTGTTTTATATGTAGTTACTTTTTCACTTTCCAGGAAAAA
AATGTAGTTACTTTTTCACTGGAACATTTTTGTATTTAGACTTCGTGCATTTTTTTTTGACCGGATAGGCTTTTAAAAATGGAGTCACTGGGTGGACTCTAAAAATGGGGTGAGTTACTATAATTAAGTTGACTTGTGGACAAACTCGGTGTATCTAGGTAAACTCTGGATAAAGTTTCACTGTTTGTATCAAATTTTGG
TTTTGAATTTTTGGTCATTTCGTGTGAATCCTTTTCTGAGGTAAAACCTTGAAATTTTGAAATTCTCTGGTGATTCC

>TRINITY_DN115697_c11_g1_i3 from sample A1 / Example unspliced isoform:

GTATGGCTTTTTAGCTTTAAAATgtatgagcctgagttggttttacagctaaatttatgatacaaacttttgatttctccctgcatacaagtttagaaatggatattcctgtttacattaatcaagtgtattcattttccgtcgccatacttttccattaaaattatctcgcatatttgtcgcagACAGGACTTTGTTTT
ACATGCATAGAATCTACGGCCACTCCTTGGTTTCGAACTTTCGAGGGACTCTAAAGCTGGGCAATGCTTGATTCTCGATTCCAATGGCGTGCAAACCCAAAATAAAATTCTCATATCTGAAAGAGAGCGTCTGTAATCTTGGCTATATAGTCATAAATCAATTAAGGTTTTTCGATGGATTTATGCTATTTTGTTTTATA
TGTAGTTACTTTTTCACTTTCCAGGAAAAAAATGTAGTTACTTTTTCACTGGAACATTTTTGTATTTAGACTTCGTGCATTTTTTTTTGACCGGATAGGCTTTTAAAAATGGAGTCACTGGGTGGACTCTAAAAATGGGGTGAGTTACTATAATTAAGTTGACTTGTGGACAAACTCGGTGTATCTAGGTAAACTCTGGA
TAAAGTTTCACTGTTTGTATCAAATTTTGGTTTTGAATTTTTGGTCATTTCGTGTGAATCCTTTTCTGAGGTAAAACCTTGAAATTTTGAAATTCTCTGGTGATTCC

Intron 2 GTAATCAATT ... TTCTTTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	68.57	11	4	24
A2	67.57	12	0	25
A3	72	14	2	36
A4	68.29	13	2	28
A5	73.33	8	0	22
A6	69.23	44	3	99
A7	67.36	47	7	97
A8	60.42	38	7	58
A9	64.71	60	8	110
A10	65.48	58	6	110

>TRINITY_DN41663_c0_g1_i1 from sample A10 / Example spliced isoform

TGTCCTCCTACGAAGCTGCCACCACAGCAATGGACGCCGATAGCTTTCACTATCCAGTATTTTCCATCACCACCTTCACCCATCAGGTGTTTGATTGTGCTTGGCAAGCTATTGAGATTTAGCAGTCTTTGTAGTCTGTGAATATGTCGGGCTTATCTCTAGCTGTGGCGCCTCGCTTGCACAGCGGGATGGGCGAAGGC
GAGAGGGATGTGAAAGGGCAAGGCTTAATGGGCGGTGTGATGGGTTCTCTGAGAGTAATCGAACTTCAGCTGGTTGCATTCATTCTGGTATTCTCGGCCAGCGGTTTGGTGCCTCTGGTAGACCTGGTATCTCCTGTATTCGTCTCGTTATATTTGCTTGCGTTGTCTCGGTGGGCGTTCCCAAGCTACAGGAAGCCCGG
CAACGCAGCGGAGGTTTTCCATGGAAGCAGAATGTTTCAGCTTTATGTTATCGTGGGCACAACGGTGGGCCTGTTCCTGCCTCTGTCCTACGTTCTTGGAGGGTTTGCCAGAGGAGACCAAGAGGCCGTGCGGTCTGCAACGCCTCATCTGTTCCTGCTTTCTTTTCAAATTCTGAGCGAGAATATCATCATGGGTTTGT
CCATATTTTCGCCGCCCGTTCGAGCTCTCGTGCCGCTTCTCTACACAGTTCGGCGTGTTTTCCTTTTAATTGACTGGACCCACGCAACGTTTTTCAATAGCAAGGTGCCCGAGAGTCCGCGCTTTCAGGATAACATATGGATCTGGTTTGGGCGAGGTCTGGCTTCTGTAAATCTTATTTATTTTACTATTAATCTCTTC
TGTTTCCTCATTCCGAGATTCCTACCGCGTGCTTTCGAGAGATACTTTCAGGAAAGAGCTGAATGTAACGGAAAAGTATCCGAAATCGAACCTCAGAAGAAGGAGAAGGCGCAGAAGAAATCTGAATGATTCACATTGTATACAAATCTGAATATGATGCATTGTGTGTGCATGACAGGACTTTGTTTTACATGCATAGA
ATCTACGGCCACTCCTTGGTTTCGAACTTTCGAGGGACTCTAAAGCTGGGCAATGCTTGATTCTCGATTCCAATGGCGTGCAAACCCAAAATAAAATTCTCATATCTGAAAGAGAGCGTCTGTAATCTTGGCTATATAGTCATAAATCAATTAAGGTTTTTCGATGGATTTATGCTATTTTGTTTTATATGTAGTTACTT
TTTCACTTTCCAGGAAAAAAATGTAGTTACTTTTTCACTGGAACATTTTTGTATTTAGACTTCGTGCATTTTTTTTTGACCGGATAGGCTTTTAAAAATGGAGTCACTGGGTGGACTCTAAAAATGGGGTGAGTTACTATAATTAAGTTGACTTGTGGACAAACTCGGTGTATCTAGGTAAACTCTGGATAAAGTTTCAC
TGTTTGTATCAAATTTTGGTTTTGAATTTTTGGTCATTTCGTGTGAATCCTT

>TRINITY_DN41663_c0_g1_i5 from sample A10 / Example unspliced isoform:

TGTCCTCCTACGAAGCTGCCACCACAGCAATGGACGCCGATAGCTTTCACTATCCAGTATTTTCCATCACCACCTTCAgtaatcaatttcttcttcgctcatggtggcctcattccagatattttgattagttttataactccaacagcaattcataatgtttggtttttctttctagCCCATCAGGTGTTTGATTGTGC
TTGGCAAGCTATTGAGATTTAGCAGTCTTTGTAGTCTGTGAATATGTCGGGCTTATCTCTAGCTGTGGCGCCTCGCTTGCACAGCGGGATGGGCGAAGGCGAGAGGGATGTGAAAGGGCAAGGCTTAATGGGCGGTGTGATGGGTTCTCTGAGAGTAATCGAACTTCAGCTGGTTGCATTCATTCTGGTATTCTCGGCCA
GCGGTTTGGTGCCTCTGGTAGACCTGGTATCTCCTGTATTCGTCTCGTTATATTTGCTTGCGTTGTCTCGGTGGGCGTTCCCAAGCTACAGGAAGCCCGGCAACGCAGCGGAGGTTTTCCATGGAAGCAGAATGTTTCAGCTTTATGTTATCGTGGGCACAACGGTGGGCCTGTTCCTGCCTCTGTCCTACGTTCTTGGA
GGGTTTGCCAGAGGAGACCAAGAGGCCGTGCGGTCTGCAACGCCTCATCTGTTCCTGCTTTCTTTTCAAATTCTGAGCGAGAATATCATCATGGGTTTGTCCATATTTTCGCCGCCCGTTCGAGCTCTCGTGCCGCTTCTCTACACAGTTCGGCGTGTTTTCCTTTTAATTGACTGGACCCACGCAACGTTTTTCAATAG
CAAGGTGCCCGAGAGTCCGCGCTTTCAGGATAACATATGGATCTGGTTTGGGCGAGGTCTGGCTTCTGTAAATCTTATTTATTTTACTATTAATCTCTTCTGTTTCCTCATTCCGAGATTCCTACCGCGTGCTTTCGAGAGATACTTTCAGGAAAGAGCTGAATGTAACGGAAAAGTATCCGAAATCGAACCTCAGAAGA
AGGAGAAGGCGCAGAAGAAATCTGAATGATTCACATTGTATACAAATCTGAATATGATGCATTGTGTGTGCATGACAGGACTTTGTTTTACATGCATAGAATCTACGGCCACTCCTTGGTTTCGAACTTTCGAGGGACTCTAAAGCTGGGCAATGCTTGATTCTCGATTCCAATGGCGTGCAAACCCAAAATAAAATTCT
CATATCTGAAAGAGAGCGTCTGTAATCTTGGCTATATAGTCATAAATCAATTAAGGTTTTTCGATGGATTTATGCTATTTTGTTTTATATGTAGTTACTTTTTCACTTTCCAGGAAAAAAATGTAGTTACTTTTTCACTGGAACATTTTTGTATTTAGACTTCGTGCATTTTTTTTTGACCGGATAGGCTTTTAAAAATG
GAGTCACTGGGTGGACTCTAAAAATGGGGTGAGTTACTATAATTAAGTTGACTTGTGGACAAACTCGGTGTATCTAGGTAAACTCTGGATAAAGTTTCACTGTTTGTATCAAATTTTGGTTTTGAATTTTTGGTCATTTCGTGTGAATCCTT

Intron 3 GTATGGCTTT ... TTTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.19	53	11.8	13.4
A2	37.73	37	22.4	13.9
A3	20.73	68.9	18	19.9
A4	20.39	46	11.8	16.7
A5	27.02	30.3	11.2	9.7
A6	25.25	518.6	175.2	98.1
A7	25.04	303.1	101.3	55.6
A8	32	308.3	145.1	76.2
A9	26.03	241.7	85.1	60.5
A10	22.73	192.3	56.6	24.3

>TRINITY_DN103083_c0_g1_i2 from sample A2 / Example spliced isoform

ATTTCTTCTTCGCTCATGGTGGCCTCATTCCAGATATTTTGATTAGTTTTATAACTCCAACAGCAATTCATAATGTTTGGTTTTTCTTTCTAGCCCATCAGGTGTTTGATTGTGCTTGGCAAGCTATTGAGATTTAGCAGTCTTTGTAGTCTGTGAATATGTCGGGCTTATCTCTAGCTGTGGCGCCTCGCTTGCACAGC
GGGATGGGCGAAGGCGAGAGGGATGTGAAAGGGCAAGGCTTAATGGGCGGTGTGATGGGTTCTCTGAGAGTAATCGAACTTCAGCTGGTTGCATTCATTCTGGTATTCTCGGCCAGCGGTTTGGTGCCTCTGGTAGACCTGGTATCTCCTGTATTCGTCTCGTTATATTTGCTTGCGTTGTCTCGGTGGGCGTTCCCAAG
CTACAGGAAGCCCGGCAACGCAGCGGAGGTTTTCCATGGAAGCAGAATGTTTCAGCTTTATGTTATCGTGGGCACAACGGTGGGCCTGTTCCTGCCTCTGTCCTACGTTCTTGGAGGGTTTGCCAGAGGAGACCAAGAGGCCGTGCGGTCTGCAACGCCTCATCTGTTCCTGCTTTCTTTTCAAATTCTGAGCGAGAATA
TCATCATGGGTTTGTCCATATTTTCGCCGCCTGTTCGAGCTCTCGTGCCGCTTCTCTACACAGTTCGGCGTGTTTTCCTTTTAATTGACTGGACCCACGCAACGTTTTTCAATAGCAAGGTGCCCGAGAGTCCGCGCTTTCAGGATAACATATGGATCTGGTTTGGACGAGGTCTGGCTTCTGCAAATCTTATTTATTTT
ACTATTAATCTCTTCTGTTTCCTCATTCCGAGATTCCTACCGCGTGCTTTCGAGAGATACTTTCAGGAAAGAGCTGAATGTAACGGAAAAGTATCCGAAATCGAACCTCAGAAGAAGGAGAAGGCGCAGAAGAAATCTGAATGATTCACATTGTATACAAATCTGAATGATGCATTGTGTGTGCATGACAGGACTTTGTT
TTACATGCATAGAATCTACGGCCACTCCTTGGTTTCGAACTTTCGAGGGACTCTAAAGCTGGGCGATGCTTGATTCTCGATTCCTATGGCGTGCAATCCCAAAATAAAATTCTCATATCTGAAAGAGAGCGTCTGTAATCTTGGCTATATAGTCATAAATCAATTAAGGTTTTTCGATGGATTTATGCTATTTTGTTTTA
TATGTAGTTACTTTTTCACTTTCCAGGAAAAAAATGTAGTTACTTTTTCACTGGAACATTTTTGTATTTAGACTTCGTGCATTTTTTTTTGACCGGATAGGCTTTTAAAAATGGAGTCACTGGGTGGACTCTAAAAATGGGGTGAGTTACTATAATTAAGTTGACTTGTGGACAAACTCGGTGTATCTAGGTAAACTCTG
GAT

>TRINITY_DN103083_c0_g1_i1 from sample A2 / Example unspliced isoform:

ATTTCTTCTTCGCTCATGGTGGCCTCATTCCAGATATTTTGATTAGTTTTATAACTCCAACAGCAATTCATAATGTTTGGTTTTTCTTTCTAGCCCATCAGGTGTTTGATTGTGCTTGGCAAGCTATTGAGATTTAGCAGTCTTTGTAGTCTGTGAATATGTCGGGCTTATCTCTAGCTGTGGCGCCTCGCTTGCACAGC
GGGATGGGCGAAGGCGAGAGGGATGTGAAAGGGCAAGGCTTAATGGGCGGTGTGATGGGTTCTCTGAGAGTAATCGAACTTCAGCTGGTTGCATTCATTCTGGTATTCTCGGCCAGCGGTTTGGTGCCTCTGGTAGACCTGGTATCTCCTGTATTCGTCTCGTTATATTTGCTTGCGTTGTCTCGGTGGGCGTTCCCAAG
CTACAGGAAGCCCGGCAACGCAGCGGAGGTTTTCCATGGAAGCAGAATGTTTCAGCTTTATGTTATCGTGGGCACAACGGTGGGCCTGTTCCTGCCTCTGTCCTACGTTCTTGGAGGGTTTGCCAGAGGAGACCAAGAGGCCGTGCGGTCTGCAACGCCTCATCTGTTCCTGCTTTCTTTTCAAATTCTGAGCGAGAATA
TCATCATGGGTTTGTCCATATTTTCGCCGCCTGTTCGAGCTCTCGTGCCGCTTCTCTACACAGTTCGGCGTGTTTTCCTTTTAATTGACTGGACCCACGCAACGTTTTTCAATAGCAAGGTGCCCGAGAGTCCGCGCTTTCAGGATAACATATGGATCTGGTTTGGACGAGGTCTGGCTTCTGCAAATCTTATTTATTTT
ACTATTAATCTCTTCTGTTTCCTCATTCCGAGATTCCTACCGCGTGCTTTCGAGAGATACTTTCAGGAAAGAGCTGAATGTAACGGAAAAGTATCCGAAATCGAACCTCAGAAGAAGGAGAAGGCGCAGAAGAAATCTGAATGATTCACATTGTATACAAATCTGAATATGATGCATTGTGTGTGCATGgtatggctttt
tagctttaaaatgtacgagcctaagtttgttttacagtttaatttatgatacaaacttttgatttctccctgcatacaagtttagaaatggatattcctgtttacattaatcaagtgtattcattttccgtcgccatacttttccattaaaattatctcgcatatttgtcgcagACAGGACTTTGTTTTACATGCATAGA
ATCTACGGCCACTCCTTGGTTTCGAACTTTCGAGGGACTCTAAAGCTGGGCAATGCTTGATTCTCGATTCCAATGGCGTGCAAACCCAAAATAAAATTCTCATATCTGAAAGAGAGCGTCTGTAATCTTGGCTATATAGTCATAAATCAATTAAGGTTTTTCGATGGATTTATGCTATTTTGTTTTATATGTAGTTACTT
TTTCACTTTCCAGGAAAAAAATGTAGTTACTTTTTCACTGGAACATTTTTGTATTTAGACTTCGTGCATTTTTTTTTGACCGGATAGGCTTTTAAAAATGGAGTCACTGGGTGGACTCTAAAAATGGGGTGAGTTACTATAATTAAGTTGACTTGTGGACAAACTCGGTGTATCTAGGTAAACTCTGGAT