sp|Q9LY87|RGLG2_ARATH ----> E3 ubiquitin-protein ligase RGLG2

Intron 1 GCAATGGGTA ... CCTTGATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.79	39.3	44	62.9
A2	50.62	36.9	29.8	45.8
A3	47.78	48.4	52	36.6
A4	55.28	30.8	37	39.1
A5	61.85	13.8	17	27.8
A6	45.41	40.8	38	30.1
A7	48.98	89.3	78.2	93.4
A8	49.13	53	42	60.2
A9	59.11	55.3	70	89.7
A10	56.21	63.1	68.8	93.2

>TRINITY_DN121742_c0_g2_i5 from sample A2 / Example spliced isoform

TGTTCCAAGCCTAGAAGAGAATGGCATATCGCGCCATCCACCAAGGCTCGAATTCGGAGAGATACAATACAGCACAATGCAAATTACACACCTCGTCGGGCAATCTCATATGGGTTGATATTCCTTATCTGAGAGTAGAAAGTGGTTGATATTCCTTACGAATATGGATAGGTCCTTTCTGGCTTTTATTCCCACGGATT
CTCTAGTGCGGGGCAAAGCAGGTGACAGCACACCCCTTCCTATTCTATCCGTGTACACTCTAATCAACTAAAATGACCGTCATAAACAGTTTCCATTTTAGCCAGACGGTCGCTGGCTTTGAAAGCATGGGAATCGGTGGGTGCTTTTGTTCACTACGAGTAACTTCGATAAATTTGGCAGAGAGGATTACGTTCTGGAA
TAGCAGCACCTTTTATATGCAACTGCCACTAATAATTCCCAGCCCGGCAAAGTTTCTCAAGTTAACAGATCTCTACAATTTTTCCGAGTCACGCTGGGCGATCTCTTCGTCATAAATCTGCCTCTGTAACTGCTGCATTGAGAGAGTCTGGACTTGAGTCTTCTAACTTGATTGTTGGTATTGACTTCACTAAGAGCAAT
GAATGGACTGGGAGACACTCTTTTAATGGGCGAAGTCTACATGAAATTGGGAGTATTATGAATCCATATGAACAAGCTATTTCTTTCATAGGGCGTACTTTGTCTCCTTTTGATGAGGACAATCTAATCCCATGTTTTGGCTTCGGTGATGGTTGGGAACTATAAAGCTGTTATCCATTTTAATTGCACGTATTCTGTTT
TGGTACATCACAATACTACTTAATATTGTCTATCTTTAGAG

>TRINITY_DN121742_c0_g2_i2 from sample A2 / Example unspliced isoform:

TGTTCCAAGCCTAGAAGAGAATGGCATATCGCGCCATCCACCAAGGCTCGAATTCGGAGAGATACAATACAGCACAATGCAAATTACACACCTCGTCGGGCAATCTCATATGGGTTGATATTCCTTATCTGAGAGTAGAAAGTGGTTGATATTCCTTACGAATATGGATAGGTCCTTTCTGGCTTTTATTCCCACGGATT
CTCTAGTGCGGGGCAAAGCAGGTGACAGCACACCCCTTCCTATTCTATCCGTGTACACTCTAATCAACTAAAATGACCGTCATAAACAGTTTCCATTTTAGCCAGACGGTCGCTGGCTTTGAAAGCATGGGAATCGGTGGGTGCTTTTGTTCACTACGAGTAACTTCGATAAATTTGGCAGAGAGGATTACGTTCTGGAA
TAGCAGCACCTTTTATATGCAACTGCCACTAATAATTCCCAGCCCGGCAAAGTTTCTCAAGTTAACAGATCTCTACAATTTTTCCGAGTCACGCTGGGCGATCTCTTCGTCATAAATCTGCCTCTgcaatgggtaataagttgacaaggaggctttcccaccaaccatcacacacccaaacatcacaattgacacccggg
cagccggttcctacaaggtcacgcacccaaagacatcagtctcaaccccaattgacatctgggcagccggttcctggaaggtcaccaacacagaaatatgcgcttattacagatcagtatgaaacccttgatcagGTAACTGCTGCATTGAGAGAGTCTGGACTTGAGTCTTCTAACTTGATTGTTGGTATTGACTTCAC
TAAGAGCAATGAATGGACTGGGAGACACTCTTTTAATGGGCGAAGTCTACATGAAATTGGGAGTATTATGAATCCATATGAACAAGCTATTTCTTTCATAGGGCGTACTTTGTCTCCTTTTGATGAGGACAATCTAATCCCATGTTTTGGCTTCGGTGATGCATCTACACATGATGTCGATGTCTTCAGCTTCTATGAAA
ACAATCGACCCTGCCATGGGTTTGAACAAGTACTTGAGCGTTACAAGGAGATTGTTCCACATCTAACATTGGCTGGTCCAACGAGTTTTGCTCCAGTAATTGAGGCTTCAATGGGCATTGTGGAAGCAAGTGGTGGTCAATATCATGTTCTTGTTATTATTGCAGATGGACAGGTAACCAGG

Intron 2 GTTGGGAACT ... CTTAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.15	84	18	12
A2	13.77	72	17	6
A3	8.08	91	14	2
A4	15.09	67.5	9	15
A5	17.17	41	9	8
A6	15.61	100	21	16
A7	13.93	262.5	65	20
A8	11.78	168.5	24	21
A9	16.15	176.5	56	12
A10	9.97	171.5	32	6

>TRINITY_DN46201_c0_g1_i7 from sample A7 / Example spliced isoform

ATTTTAGGTTCGCAATTCCATACAAGATTTAAGGTTCGCCCTGTTTGACTGCCCCAAACCCCCTTGTTCCATGCCTAGAAGAGAATGGCATATCGCGCCATCCACCAAGGCTCGAATTCGGAGAGATACAATACAGCACAATGCAAATTACACACCTCGTCGGGCAATCTCATATGGGTTGATATTCCTTATCTGAGAGT
AGAAAGTGGTTGATATTCCTTACGAATATGGATAGGTCCTTTCTGGCTTTTATTCCCACGGATTCTCTAGTACGGGGCAAAGCAGGTGACAGCACACCCCTTGCTATTCTATCCGTGTACACTCTAATCAACTAAAATGACCGTCATAAACAGTTTCCATTTTAGCCAGACGGTCGCTGGCTTTGAAAGCATGGGAATCG
GTGGGTGCTTTTGTTCACTACGAGTAACTTCGATAAATTTGGCAGAGAGGATTACGTTCTGGAATAGCAGCACCTTTTATATGCAACTGCCACTAATAATTCCCAGCCTGGCAAAGTTTCTCAAGTTAACAGATCTCTACAATTTTTCCGAGTCACGCTGGGCGATCTCTTCGTCATAAATCTGCCTCTGCAATGGGTAA
TAAGTTGACAAGGAGGCTTTCCCACCAACCATCACACACCCAAACATCACAATTGACACCCGGGCAGCCGGTTCCTACAAGGTCACGCACCCAAAGACATCAGTCTCAACCCCAATTGACATCTGGGCAGCCGGTTCCTGGAAGGTCACCAACACAGAAATATGCGCTTATTACAGATCAGTATGAAACCCTTGATCAGG
TAACTGCTGCATTGAGAGAGTCTGGACTTGAGTCTTCTAACTTGATTGTTGGTATTGACTTCACTAAGAGCAATGAATGGACTGGGAGACACTCTTTTAATGAGCGAAGTCTACATGAAATTGGGAGTATTATGAATCCATATGAACAAGCTATTTCTTTCATAGGGCGTACTTTGTCTCCTTTTGATGAGGACAATCTA
ATCCCATGTTTTGGCTTCGGTGATGCATCTACACATGATGTCGATGTCTTCAGCTTCTATGAAAACAATCGACCCTGCCATGGGTTTGAACAAGTACTTGAGCGTTACAAGGAGATTGTTCCACATCTAACATTGGCTGGTCCAACGAGTTTTGCTCCAGTAATTGAGGCTTCAATGGGCATTGTGGAAAAGAGTGGGGG
CCAGTATCATGTCCTTCTTGTTGTTGCAGATGGACAGGTAACCAGGAGTAGTGATACAAGATCCAGGCAGTTAAGCTTACAAGAACAGAAGACAATTGATGCTATAGCTACTGCAAGTCATTATCCTTTATCAATAATACTGGTTGGAGTTGGTGATGGACCCTGGGATGTGATGCAAGAATTTGATGATAAACTCCCC

>TRINITY_DN46201_c0_g1_i11 from sample A7 / Example unspliced isoform:

ATTTTAGGTTCGCAATTCCATACAAGATTTAAGGTTCGCCCTGTTTGACTGCCCCAAACCCCCTTGTTCCATGCCTAGAAGAGAATGGCATATCGCGCCATCCACCAAGGCTCGAATTCGGAGAGATACAATACAGCACAATGCAAATTACACACCTCGTCGGGCAATCTCATATGGGTTGATATTCCTTATCTGAGAGT
AGAAAGTGGTTGATATTCCTTACGAATATGGATAGGTCCTTTCTGGCTTTTATTCCCACGGATTCTCTAGTACGGGGCAAAGCAGGTGACAGCACACCCCTTGCTATTCTATCCGTGTACACTCTAATCAACTAAAATGACCGTCATAAACAGTTTCCATTTTAGCCAGACGGTCGCTGGCTTTGAAAGCATGGGAATCG
GTGGGTGCTTTTGTTCACTACGAGTAACTTCGATAAATTTGGCAGAGAGGATTACGTTCTGGAATAGCAGCACCTTTTATATGCAACTGCCACTAATAATTCCCAGCCTGGCAAAGTTTCTCAAGTTAACAGATCTCTACAATTTTTCCGAGTCACGCTGGGCGATCTCTTCGTCATAAATCTGCCTCTGCAATGGGTAA
TAAGTTGACAAGGAGGCTTTCCCACCAACCATCACACACCCAAACATCACAATTGACACCCGGGCAGCCGGTTCCTACAAGGTCACGCACCCAAAGACATCAGTCTCAACCCCAATTGACATCTGGGCAGCCGGTTCCTGGAAGGTCACCAACACAGAAATATGCGCTTATTACAGATCAGTATGAAACCCTTGATCAGG
TAACTGCTGCATTGAGAGAGTCTGGACTTGAGTCTTCTAACTTGATTGTTGGTATTGACTTCACTAAGAGCAATGAATGGACTGGGAGACACTCTTTTAATGAGCGAAGTCTACATGAAATTGGGAGTATTATGAATCCATATGAACAAGCTATTTCTTTCATAGGGCGTACTTTGTCTCCTTTTGATGAGGACAATCTA
ATCCCATGTTTTGGCTTCGGTGATGgttgggaactataaagctgttatccattttaattgcacgtattctgttttggtacatcacaatactacttaatattgtctatctttagaggcaattgtattaatatctagtaatgcttagtgcagCATCTACACATGATGTCGATGTCTTCAGCTTCTATGAAAACAATCGACCC
TGCCATGGGTTTGAACAAGTACTTGAGCGTTACAAGGAGATTGTTCCACATCTAACATTGGCTGGTCCAACGAGTTTTGCTCCAGTAATTGAGGCTTCAATGGGCATTGTGGAAAAGAGTGGGGGCCAGTATCATGTCCTTCTTGTTGTTGCAGATGGACAGGTAACCAGGAGTAGTGATACAAGATCCAGGCAGTTAAG
CTTACAAGAACAGAAGACAATTGATGCTATAGCTACTGCAAGTCATTATCCTTTATCAATAATACTGGTTGGAGTTGGTGATGGACCCTGGGATGTGATGCAAGAATTTGATGATAAACTCCCC