NA ----> NA

Intron 1 GTACGTGCTC ... GTCTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.89	3	36	32
A2	88.24	3	22	23
A3	80.56	7	28	30
A4	80.65	6	27	23
A5	77.78	3	14	7
A6	83.37	43	188	243
A7	84.68	35	179	208
A8	76.36	85	240	309
A9	78.51	52	185	195
A10	81.91	34	154	154

>TRINITY_DN99938_c0_g1_i2 from sample A4 / Example spliced isoform

TCTCCAATCACTCCAGAAGACTTTACTAGAAGTTACAAGCAAATATTGTATGGAGGTCTTTCCTTGGTATTTCTTTCTTTTGTGTGGAGTTCAAGCTCAGCTCTTCTGTCGGGAACATACGAGAAGCTGACACCGTTAAGGAGATTGAAGATAAATCAGATTAAGATCTTTAATCTGAAGCATCAAAACGTTGATTCAAA
CTTTACTGAATCAGGGTTGAAAGTGCTGCTTGTTTTATAGGCTTATGGAATAAATGATTGTAATATATGATGGATGATTTCATCTCCTGTACATAAGTTTGTCTTGTATCATTTTCCTTGACTGGAAGAAAGCATGATGGGAAACCCTCTATTCAATAAATTCGTTGTATATTGTATGGCCCATATGCACTGTAAAAGTT
ATTCACATGTAATGATCTCCCTCAGACGTACACACAAAGTAGTTGATCTATGGATTTGTAATGAAAGACCCCCTTGTCACAAGC

>TRINITY_DN99938_c0_g1_i5 from sample A4 / Example unspliced isoform:

CTTCAACATTGCAATCATTTGGGATTTGAGCTGCCCCAACTTCCCTGAAATAATTGAAGAAACCCCAATAATATATTGCAGGACCCCCAATAATTGGAGGCTGTTAGCACTTTTGAGCGGCAATTTCTTCACATTACAGCGGCAATTTCTTCACATTACAGGCATCATTTGAATGGCAAATATTTGTCAAGCCTTGTCAT
CTATGGATGTGGCAGTCAAATATGGAGGACGTTTGACTTGTTTTGGGCACTTGGATGTAAAATCTGGTTCCCAACTACCAACTTTTTCACCTGTGGATACATTCACATCCCATTTCACAGTCAACCGAAATTTTTTGGTGAGAAATCCAAATTTAAGATTCAGTGCGTCATGGTTCAGGGCATCTCAAACTGCACAACCG
GCTGTGGCAGGTCCATTGAAGTCAGAGCTATGGGGGGATCTTCTGAACATAAGAAACTCTCCTAAGAAGGTTGGAAAGGTTAAGCAGAGAAAAGTTAGATGGAACTGCATTAGTGGTTCATATGGTGGAAGGTCTTCAGATGAACCTGTGCCTGGAGGAGATCCAAATACTCAGGATATTCTTGTTGAGATGGTTAATCT
GGAAATATCCAAGGTTCAGATGATAGAGATTGTAGATGAAAGTTCCCGAAAGATACAAAATATTGGACTACAAACCCAGCTGGAATATGCTCAACTTGCTGATCTAACTGTGAAAAAATTGGATGCATCTGGGAACCAGGTTCTCAGCCTACTAGATGTAAATGCCAACGCATGCCTGCATGAGTTTGATCTTGCTACTG
CAGATCTTGAGGCCCAATCTCGCAATTTTGAGCAGCTTCAGCTTCTTGCAGCATATTCAAGAAACGATGGGCTGTTCTTCAAAAATCTGTATAAAGCACCGCCAGTACTTGCATATAGATCAGATCTTTCAACGTCTTTGACAGAAGAGAAAGCACCGGTCTCATCTCCAATCACTCCAGAAGACTTTACCAGAAGAGAA
AGCACCGGTCTCATCTCCAATCACTCCAGAAGACTTTACTAGAAGTTACAAGCAAATATTGTATGGAGGTCTTTCCTTGGTATTTCTTTCTTTTGTGTGGAGTTCAAGCTCAGCTCTTCTGTCGGGAACATACgtacgtgctccaaaactaatatcttatggggtcatcatgtctttgcttctgttacaattagcttatg
caaaagtcttgacagGAGAAGCTGACACCGTTAAGGAGATTGAAGATAAATCAGATTAAGATCTTTAATCTGAAGCATCAAAACGTTGATTCAAACTTTACTGAATCAGGGTTGAAAGTGCTGCTTGTTTTATAGGCTTATGGAATAAATGATTGTAATATATGATGGATGATTTCATCTCCTGTACATAAGTTTGTCTT
GTATCATTTTCCTTGACTGGAAGAAAGCATGATGGGAAACCCTCTATTCAATAAATTCGTTGTATATTGTATGGCCCATATGCACTGTAAAAGTTATTCACATGTAATGATCTCCCTCAGACGTACACACAAAGTAGTTGATCTATGGATTTGTAATGAAAGACCCCCTTGTCACAAGC

Intron 2 GTAAAAGCCC ... TTTTCTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.41	36	5.5	15
A2	24.24	25	5.5	8
A3	29.27	29	3	12
A4	25	24	3.5	8
A5	20	20	5	5
A6	28.57	185	26.5	74
A7	25.33	171	18.5	58
A8	24.04	218	24.5	69
A9	26.16	175	17.5	62
A10	26.32	140	7	50

>TRINITY_DN43110_c0_g1_i2 from sample A7 / Example spliced isoform

TATAAACAAAATATCTTACAAAATTCCGTAGAAGGAATCAATGGAGTGTGAGAGAGAGATTCAATGAATCTCCACCGCGTGCTCTGATGGTGACAATATTTGTTTCCGTGTCTTTCGACGGCAAGAATATGCACACGTTAAAGTTCTCCTCGCCCACCACCATTCGACATCTTTTCTATTAAACTCTTCAACATTGCAAT
CATTTGGGATTTGAGCTGCCCCAACTTCCCTGAAATAATTGAAGAAACCCCAATAATATATTGCAGGACCCCCAATAATTGGAGGCTGTTAGCACTTTTGAGCGGCAATTTCTTCACATTACAGCGGCAATTTCTTCACATTACAGGCATCATTTGAATGGCAAATATTTGTCAAGCCTTGTCATCTATGGATGTGGCAG
TCAAATATGGAGGACGTTTGACTTGTTTTGGGCACTTGGATGTAAAATCTGGTTCCCAACTACCAACTTTTTCACCTGTGGATACATTCACATCCCATTTCACAGTCAACCGAAATTTTTTGGTGAGAAATCCAAATTTAAGATTCAGTGCGTCATGGTTCAGGGCATCTCAAACTGCACAACCGGCTGTGGCAGGTCCA
TTGAAGTCAGAGCTATGGGGGGATCTTCTGAACATAAGAAACTCTCCTAAGAAGGTTGGAAAGGTTAAACAGAGAAAAGTTAGATGGAACTGCATTAGTGGTTCATATGGTGGAAGGTCTTCAGATGAACCTGTGCCTGGAGGAGATCCAAATACTCAGGATATTCTTGTTGAGATGGTTAATCTGGAAATATCCAAGGT
TCAGATGATAGAGATTGTAGATGAAAGTTCCCGAAAGATACAAAATATTGGACTACAAACCCAGCTGGAATATGCTCAACTTGCTGATCTAACTGTGAAAAAATTGGATGCATCTGGGAACCAGGTTCTCAGCCTACTAGATGTAAATGCCAACGCATGCCTGCATGAGTTTGATCTTGCTACTGCAGATCTTGAGGCCC
AATCTCGCAATTTTGAGCAGCTTCAGCTTCTTGCAGCATATTCAAGAAACGATGGGCTGTTCTTCAAAAATCTGTATAAAGCACCGCCAGTACTTGCATATAGATCAGATCTTTCAACGTCTTTGACAGAAGAGAAAGCACCGGTCTCATCTCCAATCACTCCAGAAGACTTTACTAGAAGTTACAAGCAAATATTGTAT
GGAGGTCTTTCCTTGGTATTTCTTTCTTTTGTGTGGAGTTCAAGCTCAGCTCTTCTGTCGGGAACATACGTACGTGCTCCAAAACTAATATCTTATGGGGTCATCATGTCTTTGCTTCTGTTACAATTAGCTTATGCAAAAGTCTTGACAGGAGAAGCTGACACTGTTAAGGAGATTGAAGATAAATCAGATTAAGATCT
TTAATCTGAAGCATCAAAACGTTGATTCAAACTTTACTGAATCAGGGTTGAAAGTGCTGCTTGTTTTATAGGCTTATGGAATAAATGATTGTAATATATGATGGATGATTTCATCTCCTGTACATAAGTTTGTCTTGTATCATTTTCCTTGACTGGAAGAAAGCATGATGGGAAACCCTCTATTCAATAAATTCGTTGTA
TATTGTATGGCCCATATGCACTGTAAAAGTTATTCACATGTAATGATCTCCCTCAGACGTACACACAAAGTAGTTGATCTATGGATTTGTAATGAAAGACCCCCTTGTCACAAGTAGGCTGCTGCATCACCATAAACCAACTTTTGGTGAAAATTCCAAAGGAATTGGCGGGTTTAGTTACTTTGACTATATTAAACTAT
GAATAAATAGCATGAAAAACATGAAATTGATAGTGTTTGGTATAGTTGCATTCACCATCCTTAGGGAAAATTTTACATCTCAAATTGATTCTGTACGACTGTGGTCCAGTCCCTGCCTTGGTGGTTGAGATAGGATGTTGTCACCATAATGATCCAAGTTCAAACCTCTGTGGGCATAGAGTTCTGATCATGTGTATAAG
TCTTCAAGTTTGACGATCCCATGTTAAAGTGGGCATTTACCAGAATAATGCTGCGATGGGGGTTATAACTCACCAATCAAAACAAACCTCTACCCTTTTCAAAGCAGAAAAAGACATCGCATGAATTTTGAGAGGAAACATTCTACCACGATGTAATTACAAAAGATGTGAATAATACGAC

>TRINITY_DN43110_c0_g1_i1 from sample A7 / Example unspliced isoform:

TATAAACAAAATATCTTACAAAATTCCGTAGAAGGAATCAATGGAGTGTGAGAGAGAGATTCAATGAATCTCCACCGCGTGCTCTGATGGTGACAATATTTGTTTCCGTGTCTTTCGACGGCAAGAATATGCACACGTTAAAGTTCTCCTCGCCCACCACCATTCGACATCTTTTCTATTAAACTCTTCAACATTGCAAT
CATTTGGGATTTGAGCTGCCCCAACTTCCCTGAAATAATTGAAGAAACCCCAATAATATATTGCAGGACCCCCAATAATTGGAGGCTGTTAGCACTTTTGAGCGGCAATTTCTTCACATTACAGCGGCAATTTCTTCACATTACAGGCATCATTTGAATGGCAAATATTTGTCAAGCCTTGTCATCTATGGATGTGGCAG
TCAAATATGGAGGACGTTTGACTTGTTTTGGGCACTTGGATGTAAAATCTGGTTCCCAACTACCAACTTTTTCACCTGTGGATACATTCACATCCCATTTCACAGTCAACCGAAATTTTTTGGTGAGAAATCCAAATTTAAGATTCAGTGCGTCATGGTTCAGGGCATCTCAAACTGCACAACCGGCTGTGGCAGGTCCA
TTGAAGTCAGAGCTATGGGGGGATCTTCTGAACATAAGAAACTCTCCTAAGAAGGTTGGAAAGGTTAAACAGAGAAAAGTTAGATGGAACTGCATTAGTGGTTCATATGGTGGAAGGTCTTCAGATGAACCTGTGCCTGGAGGAGATCCAAATACTCAGGATATTCTTGTTGAGATGGTTAATCTGGAAATATCCAAGGT
TCAGATGATAGAGATTGTAGATGAAAGTTCCCGAAAGATACAAAATATTGGACTACAAACCCAGCTGGAATATGCTCAACTTGCTGATCTAACTGTGAAAAAATTGGATGCATCTGGGAACCAGGTTCTCAGCCTACTAGATGTAAATGCCAACGCATGCCTGCATGAGTTTGATCTTGCTACTGCAGATCTTGAGGCCC
AATCTCGCAATTTTGAGCAGCTTCAGCTTCTTGCAGCATATTCAAGAAACGATGGGCTGTTCTTCAAAAATCTGTATAAAGCACCGCCAGTACTTGCATATAGATCAGATCTTTCAACGTCTTTGACAGAAGAGAAAGCACCGGTCTCATCTCCAATCACTCCAGAAGACTTTACTAGAAGTTACAAGCAAATATTGTAT
GGAGGTCTTTCCTTGGTATTTCTTTCTTTTGTGTGGAGTTCAAGCTCAGgtaaaagcccacaaccattcttatattttattaagacgtttggacatacaattttcgacattttgagttagtttctgccactgcactgattgttgcccttttcaaattttctgtagCTCTTCTGTCGGGAACATACGTACGTGCTCCAAAA
CTAATATCTTATGGGGTCATCATGTCTTTGCTTCTGTTACAATTAGCTTATGCAAAAGTCTTGACAGGAGAAGCTGACACTGTTAAGGAGATTGAAGATAAATCAGATTAAGATCTTTAATCTGAAGCATCAAAACGTTGATTCAAACTTTACTGAATCAGGGTTGAAAGTGCTGCTTGTTTTATAGGCTTATGGAATAA
ATGATTGTAATATATGATGGATGATTTCATCTCCTGTACATAAGTTTGTCTTGTATCATTTTCCTTGACTGGAAGAAAGCATGATGGGAAACCCTCTATTCAATAAATTCGTTGTATATTGTATGGCCCATATGCACTGTAAAAGTTATTCACATGTAATGATCTCCCTCAGACGTACACACAAAGTAGTTGATCTATGG
ATTTGTAATGAAAGACCCCCTTGTCACAAGTAGGCTGCTGCATCACCATAAACCAACTTTTGGTGAAAATTCCAAAGGAATTGGCGGGTTTAGTTACTTTGACTATATTAAACTATGAATAAATAGCATGAAAAACATGAAATTGATAGTGTTTGGTATAGTTGCATTCACCATCCTTAGGGAAAATTTTACATCTCAAA
TTGATTCTGTACGACTGTGGTCCAGTCCCTGCCTTGGTGGTTGAGATAGGATGTTGTCACCATAATGATCCAAGTTCAAACCTCTGTGGGCATAGAGTTCTGATCATGTGTATAAGTCTTCAAGTTTGACGATCCCATGTTAAAGTGGGCATTTACCAGAATAATGCTGCGATGGGGGTTATAACTCACCAATCAAAACA
AACCTCTACCCTTTTCAAAGCAGAAAAAGACATCGCATGAATTTTGAGAGGAAACATTCTACCACGATGTAATTACAAAAGATGTGAATAATACGAC

Intron 3 GTATGGAGGC ... TCAAGCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.62	20	31	36
A2	56.52	20	27	25
A3	64.84	16	30	29
A4	67.57	12	26	24
A5	65.08	11	21	20
A6	62.96	95	138	185
A7	64.08	88	143	171
A8	64.93	111	193	218
A9	65.06	87	149	175
A10	65.61	68	119	140.5

>TRINITY_DN108684_c0_g1_i4 from sample A2 / Example spliced isoform

GCACACGTTAAAGTTCTCCTCGCCCACCACCATTCGACATCTTTTCTATTAAACTCTTCAACATTGCAATCATTTGGGATTTGAGCTGCCCCAACTTCCCTGAAATAATTGAAGAAACCCCAATAATATATTGCAGGACCCCAATAATTGGAGGCTGTTAGCACTTTTGAGCGGCAATTTCTTCACATTACAGCGGCAAT
TTCTTCACATTACAGGCATCATTTGAATGGCAAATATTTGTCAAGCCTTGTCATCTATGGATGTGGCAGTCAAATATGGAGGACGTTTGACTTGTTTTGGGCACTTGGATGTAAAATCTGGTTCCCAACTACCAACTTTTTCACCTGTGGATACATTCACATCCCATTTCACAGTCAACCGAAATTTTTTGGTGAGAAAT
CCAAATTTAAGATTCAGTGCGTCATGGTTCAGGGCATCTCAAACTGCACAACCGGCTGTGGCAGGTCCATTGAAGTCAGAGCTATGGGGGGATCTTCTGAACATAAGAAACTCTCCTAAGAAGGTTGGAAAGGTTAAACAGAGAAAAGTTAGATGGAACTGCATTAGTGGTTCATATGGTGGAAGGTCTTCAGATGAACC
TGTGCCTGGAGGAGATCCAAACACTCAGGATATTCTTGTTGAGATGGTTAATCTGGAAATATCCAAGGTTCAGATGATAGAGATTGTAGATGAAAGTTCCCGAAAGATACAAAATATTGGACTACAAACCCAGCTGGAATATGCTCAACTTGCTGATCTAACTGTGAAAAAATTGGATGCATCTGGGAACCAGGTTCTCA
GCCTACTAGATGTAAATGCCAACGCATGCCTGCATGAGTTTGATCTTGCTACTGCAGATCTTGAGGCCCAATCTCGCAATTTTGAGCAGCTTCAGCTTCTTGCAGCATATTCAAGAAACGATGGGCTGTTCTTCAAAAATCTGTATAAAGCACCGCCAGTACTTGCATATAGATCAGATCTTTCAACGTCTTTGACAGAA
GAGAAAGCACCGGTCTCATCTTCAATCACTCCAGAAGACTTTACTAGAAGTTACAAGCAAATATTCTCTTCTGTCGGGAACATACGTACGTGCTCCAAAACTAATATCTTATGGGGTCATCATGTCTTTGCTTCTGTTACAATTAGCTTATGCAAAAGTCTTGACAGGAGAAGCTGACACTGTTAAGGAGATTGAAGATA
AATCAGATTAAGATCTTTAATCTGAAGCATCAAAACGTTGATTCAAACTTTACTGAATCAGGGTTGAAAGTGCTGCTTGTTTTATAGGCTTATGGAATAAATGATTGTAATATATGATGGATGATTTCATCTCCTGTACATAAGTTTGTCTTGTATCATTTTCCTTGACTGGAAGAAAGCATGATGGGAAACCCTCTATT
CAATAAATTCGTTGTATATTGTATGGCCCATATGCACTGTAAAAGTTATTCACATGTAATGATCTCCCTCAGACGTACACACAAAGTAGTTGATCTATGGATTTGTAATGATCTCCCTCAGACGTACACACAAAGTAGTTGATCTATGGATTTGTAATGAAAGACCCCCTTGTCACAAGTAGGCTGCTGCATCACCATAA
ACCAACTTTTGGTGAAAATTCCAAAGGAATTGGCGGG

>TRINITY_DN108684_c0_g1_i3 from sample A2 / Example unspliced isoform:

GTTGATATATGAGGTCTTGTAGAATTGTTAGATTTTATTGATCCATGTTCAACTTTTCAGGCCCAATCTCGCAATTTTGAGCAGCTTCAGCTTCTTGCAGCATATTCAAGAAACGATGGGCTGTTCTTCAAAAATCTGTATAAAGCACCGCCAGTACTTGCATATAGATCAGATCTTTCAACGTCTTTGACAGAAGAGAA
AGCACCGGTCTCATCTTCAATCACTCCAGAAGACTTTACTAGAAGTTACAAGCAAATATTgtatggaggcctttccttggtatttctttcttttgtgtggagttcaagctcagCTCTTCTGTCGGGAACATACGTACGTGCTCCAAAACTAATATCTTATGGGGTCATCATGTCTTTGCTTCTGTTACAATTAGCTTATG
CAAAAGTCTTGACAGGAGAAGCTGACACTGTTAAGGAGATTGAAGATAAATCAGATTAAGATCTTTAATCTGAAGCATCAAAACGTTGATTCAAACTTTACTGAATCAGGGTTGAAAGTGCTGCTTGTTTTATAGGCTTATGGAATAAATGATTGTAATATATGATGGATGATTTCATCTCCTGTACATAAGTTTGTCTT
GTATCATTTTCCTTGACTGGAAGAAAGCATGATGGGAAACCCTCTATTCAATAAATTCGTTGTATATTGTATGGCCCATATGCACTGTAAAAGTTATTCACATGTAATGATCTCCCTCAGACGTACACACAAAGTAGTTGATCTATGGATTTGTAATGATCTCCCTCAGACGTACACACAAAGTAGTTGATCTATGGATT
TGTAATGAAAGACCCCCTTGTCACAAGTAGGCTGCTGCATCACCATAAACCAACTTTTGGTGAAAATTCCAAAGGAATTGGCGGG