NA ----> NA

Intron 1 GTGAACTAAA ... ATTTTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	0	4
A2	14.29	3	0	1
A3	27.27	4	2	1
A4	0	1	0	0
A5	0	2	0	0
A6	17.78	37	4	12
A7	12.1	218	34	26
A8	14.02	227	35	39
A9	10.22	347	30	49
A10	9.15	263	25	28

>TRINITY_DN47232_c0_g3_i7 from sample A7 / Example spliced isoform

CCTGTTTCTGTGTTGAGGTTCACTTGTGTGACCCCGATCCAACTCCCGGAATCCTGTTTTGGTTGAAAGATCGAACAAATTGCAGGTTATTTGGTTGACAGATCAGAACAAATTGCAGGTTGATATTATTATTGCTGAAAATGACTTGGCAAGAGGAGTGGAATACATTTCTTGCCGAGGCAGAGAAAGACGAAGAATAT
GACGAGAAAGAAGACGAAGACGAAGACGAGAAAGAGGACGAGGTGAACTCACACGAAAATGACGAGAAAGAGGACGAGGATGACGAGAAAGAAAACGAGGTGAACTCAGACGAAAATGACGAGAAAGAAATCGAGGTGAACTCAGACGAAGACGAGGGCGATATTTTTCCAGTGCCTACACAAATCCTTGGTTTGGGAAT
GGAAATGCAGAAACGAAACAGCGAAAACGAAACTGAAATGGAGAAATCGGGCAGTGAAAAAGGAGCTAATTCGAAGGAAAGACTAAAAAGGTCGTTCTGGTGGACTCCTAATTGTTCCAGCAAGCGGCGGGCCTGGGCTGAAGCATTCAGTGCTTTGTAAATATGGCATTGCTATTACCTGCCAATCAAATCAATCATAC
TAAT

>TRINITY_DN47232_c0_g3_i3 from sample A7 / Example unspliced isoform:

CGAAGACGAAGACGAGAAAGAGGACGAGGTGAACTCACACGAAAATGACGAGAAAGAGGACGAGGATGACGAGAAAGAAAACGAGGTGAACTCAGACGAAAATGACGAGAAAGAAATCGAGGTGAACTCAGACGAAGACGAGgtgaactaaaaattccgttccctaccctaaccctacaatatatagatctctttggcat
tctgaattagtcattacatcttcttggctttttggttttaaatgggtacttcttgttttattttaacagGGCGATATTTTTCCAGTGCCTACACAAATCCTTGGTTTGGGAATGGAAATGCAGAAACGAAACAGCGAAAACGAAACTGAAATGGAGAAATCGGGCAGTGAAAAAGGAGCTAATTCGAAGGAAAGACTAAA
AAGGTCGTTCTGGTGGACTCCTAATTGTTCCAGCAAGCGGCGGGCCTGGGCTGAAGCATTCAGTGCTTTGTAAATATGGCATTGCTATTACCTGCCAATCAAATCAATCATACTAAT

Intron 2 GTGAACTCAG ... ATTTTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.29	11	8	4
A2	48.23	11	14.5	6
A3	49.62	19	27	10.5
A4	40.84	9	9.5	3
A5	29.91	8	5	2
A6	22.83	147	58	29
A7	24.09	397	157	95
A8	21.63	461	139.5	115
A9	26.17	412	180.5	112
A10	28.29	300	165	72

>TRINITY_DN44550_c0_g2_i11 from sample A8 / Example spliced isoform

ATTGCTGAAAATGACTTGGCAAGGTAGGGTTATACAATGTATCATTTTACTGTCCATTTAGAAGTGAAATTTCAAGAAATTTTAACGTGGCGATGTATGTGCAGAGGAGTGGAATACATTTCTTGTCGTGGCAGAGAAAGACGAAGTATATTTCTCCGAGGATGACGAGAAAGAAGACGAAGACCAAGACGAGGACGATA
TTTTTCCAGTGCCTGCACAAATCCATGTTTTCGAAAAGGAAATGGAGAAACCAAGCAGTGAAAAAGAAACTGAAATAGAGAAATCGGGCCGAGAAAAAGGAGCTGAATCGAAGGAAAGACGAAAAAGGTCGTTCTGCTGGACTCCTAATTGTTCAAGCAAGCGGCGGGCCTTGGCTGAAGCATTCAGTGCTTTGTAAATA
TGGCATTGCTATTAACTGCCAATCAAATCAATCATACTAATATTTTAAAATGAAACAAAACATTTCTGTCTTGTAATTCTCTTGTACTTTTATATAAAAAATTGTCTTTCATAAATATATGAATAGTCATTTAATAATGATCTTGTACTTTTATATAAAAAATTGTCTTTCATAAATATATGAATAGTCATTTAATAATG
ATCTTGTACTTTTATATAAAAAAT

>TRINITY_DN44550_c0_g2_i9 from sample A8 / Example unspliced isoform:

ATTGCTGAAAATGACTTGGCAAGGTAGGGTTATACAATGTATCATTTTACTGTCAATTTAGCAGTGAAATTTCAAGAAATAAATTTTAACGTGGCTGATGTATGTGCAGAGGAGTGGAATACATTTCTTGTCGTGGCAGAGAAAGACGAAGTATATTTCTCCGAGGATGACGAGAAAGAAGACGAAGACCAAGACGAGgt
gaactcagacgaagacgaagacggggtgaactaaaaattccgctccctacccttaccctaaccctaaccctaaaatatatacatctttttggctttctgaattagtcattacatcttcttggctttctgaattagtcattacatcttcttggctttttggttttaaatggctacttcgttttattttaacagGACGATAT
TTTTCCAGTGCCTGCACAAATCCATGTTTTCGAAAAGGAAATGGAGAAACCAAGCAGTGAAAAAGAAACTGAAATAGAGAAATCGGGCCGAGAAAAAGGAGCTGAATCGAAGGAAAGACGAAAAAGGTCGTTCTGCTGGACTCCTAATTGTTCAAGCAAGCGGCGGGCCTTGGCTGAAGCATTCAGTGCTTTGTAAATAT
GGCATTGCTATTAACTGCCAATCAAATCAATCATACTAATATTTTAAAATGAAACAAAACATTTCTGTCTTGTAATTCTCTTGTACTTTTATATAAAAAATTGTCTTTCATAAATATATGAATAGTCATTTAATAATGATCTTGTACTTTTATATAAAAAATTGTCTTTCATAAATATATGAATAGTCATTTAATAATGA
TCTTGTACTTTTATATAAAAAAT

Intron 3 GTGAACTAAA ... ATTTTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	0	4
A2	42.86	2	0	3
A3	27.27	4	2	1
A4	20	2	0	1
A5	0	2	0	0
A6	32.5	27	4	22
A7	25.2	141	34	61
A8	30.2	141	35	87
A9	25.96	221	30	125
A10	19.17	213	25	76

>TRINITY_DN44550_c0_g2_i4 from sample A8 / Example spliced isoform

GGTGAACTCACACGAAAATGACGAGAAAGAGGACGAGGATGACGAAGACGAGAAAGAGGACGAGGTGAACTCACACGAAAATGACGAGAAAGAGGACGAGGATGACGAGAAAGAAAACGAGGTGAACTCAGACGAAAATGACGACAAAGAAAACGAGGAGAACTCAGACGAAGACGAGTGCCTACACAAATCCTTGGTTT
GGGAATGGAAATGCGGAAACCAAACAGCGAAAACGAAACTGAAATGGAGAAATCGGGCAGTGAAAAAGGAGCTAATTCGAAGGAAAGACTAAAAAGGTCGTTCTGGTGGACTCCTAATTGTTCCAAAGCAAGCGGCGGGCCTTGCAAGCGGCGGGCCTGGGCTGAAGCATTCAGTGCTTTGTAAATATGGCATTGCTACT
GCCAATCAAATCAATCAGATTAATGTCTTTTCAGTCTGAAACAATTTTGTCTTGTAAGTCTCTTTTACTTTTATATAAAAAAATGGTCTTTCTCAGTTCTTTGAAGGCATAAATATAT

>TRINITY_DN44550_c0_g2_i20 from sample A8 / Example unspliced isoform:

GGTGAACTCACACGAAAATGACGAGAAAGAGGACGAGGATGACGAAGACGAGAAAGAGGACGAGGTGAACTCACACGAAAATGACGAGAAAGAGGACGAGGATGACGAGAAAGAAAACGAGGTGAACTCAGACGAAAATGACGAGAAAGAAATCGAGGTGAACTCAGACGAAGACGAGgtgaactaaaaattccgttccc
taccctaaccctacaatatatagatctctttggcattctgaattagtcattacatcttcttggctttttggttttaaatgggtacttcttgttttattttaacagggcgatatttttccagTGCCTACACAAATCCTTGGTTTGGGAATGGAAATGCGGAAACCAAACAGCGAAAACGAAACTGAAATGGAGAAATCGGG
CAGTGAAAAAGGAGCTAATTCGAAGGAAAGACTAAAAAGGTCGTTCTGGTGGACTCCTAATTGTTCCAAAGCAAGCGGCGGGCCTTGCAAGCGGCGGGCCTGGGCTGAAGCATTCAGTGCTTTGTAAATATGGCATTGCTACTGCCAATCAAATCAATCAGATTAATGTCTTTTCAGTCTGAAACAATTTTGTCTTGTAA
GTCTCTTTTACTTTTATATAAAAAAATGGTCTTTCTCAGTTCTTTGAAGGCATAAATATAT

Intron 4 GTTGATATTA ... ACTTGGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	100	0	2	2
A3	100	0	1	1
A4	-1	0	0	0
A5	-1	0	0	0
A6	84.62	4	24	20
A7	97.87	2	97	87
A8	100	0	85	82
A9	98.78	2	165	158
A10	100	0	107	105

>TRINITY_DN42846_c0_g1_i1 from sample A9 / Example spliced isoform

GTTCACTTCTGTCACCCTCATCCAACTCCCCGAATCTTGTTTTGGTAGAAAGATCGGAACAAATAGCAAGTTATTTGGTTGGCAGATCACAACAAATTGCAGAGGAGTGGAATAAAATTGTTGCCGAGGCAAAGAAAAACAATGTAGCAGCAAGCGGCGGGCCTGGGCTGAAGCATTCAGTGCTTTGGATATTTCTTCGA
CGATGACGAGAAAAAAAAAATTTCTTCGACGATGACGAGAAAGAAGACGAAGACAAAGACGAGGTGAACTCAGACGAAGACAAGGACGATATTTTTCCAGTGCCTGCACAAATCAGTGGTTTGGGAATGGTAATGGAGACACCAAGCAGCGAAAACGAAGCTGAAATGGAGAAATCTGGCAGTGAAAAAGAAGCTGAATC
GAAGGAAAGACGAAAAAGGTTGTTCTGGTGGACTCCTAATTGTTCCAGACAAGCGGCGGGCCTGGGCTGAAGCATTCAGTGCTTTGTAAATATGGCATTGCAATTACCTGCCAATCGAATCAATCATACTAATATTTTTCATTCTAACAAAATTGGTTTGTGTTATTCTCTTTTACTTTTATATCAAAATGGTTCTTCTT
AAATTCTT

>TRINITY_DN42846_c0_g1_i9 from sample A9 / Example unspliced isoform:

GTTCACTTCTGTCACCCTCATCCAACTCCCCGAATCTTGTTTTGGTAGAAAGATCGGAACAAATAGCAAGTTATTTGGTTGGCAGATCACAACAAATTGCAGgttgatattattattgctgaaaatgacttggcaagAGGAGTGGAATAAAATTGTTGCCGAGGCAAAGAAAAACAATGTAGCAGCAAGCGGCGGGCCTG
GGCTGAAGCATTCAGTGCTTTGGATATTTCTTCGACGATGACGAGAAAAAAAAAATTTCTTCGACGATGACGAGAAAGAAGACGAAGACAAAGACGAGGTGAACTCAGACGAAGACAAGGACGATATTTTTCCAGTGCCTGCACAAATCAGTGGTTTGGGAATGGTAATGGAGACACCAAGCAGCGAAAACGAAGCTGAA
ATGGAGAAATCTGGCAGTGAAAAAGAAGCTGAATCGAAGGAAAGACGAAAAAGGTTGTTCTGGTGGACTCCTAATTGTTCCAGACAAGCGGCGGGCCTGGGCTGAAGCATTCAGTGCTTTGTAAATATGGCATTGCAATTACCTGCCAATCGAATCAATCATACTAATATTTTTCATTCTAACAAAATTGGTTTGTGTTA
TTCTCTTTTACTTTTATATCAAAATGGTTCTTCTTAAATTCTT

Intron 5 GTGAACCCTA ... ATTTGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	1	1	4
A2	100	0	0	1
A3	42.86	2	0	3
A4	-1	0	0	0
A5	20	2	0	1
A6	6.67	14	0	2
A7	19.02	83	21	18
A8	18	82	16	20
A9	17.01	122	26	24
A10	17.16	140	29	29

>TRINITY_DN47556_c1_g3_i2 from sample A10 / Example spliced isoform

GCTGTCAATACACAAGCTAGAACCATGATTTCGAATTTCAAATTACCTTTTAAGACACCTTTGGTGAGAATATACATAGTCTATTTGGTTTCATTGGATCCAGTCTTCCGCACATCAAAACTCCCCGTTTCAGTGTTGAGGTTCAGTTGTGTCACCAAGATCCAACTCCCCAAATCTTGTTTTGGTCGAAAGATCGGAAC
AAATTGCAGGTTATTTGGTTCAGAGATCAGAACAAATTGCAGGTTGATATTATTATTGCTGAAAATGACTTGGCAAGAGGAGTGGAATACATTTCTTGCCGTGGCAGAGAAAGACGAAGTATATTTCTCCGAGGATGAGGATGACGAGAGAGAAGACGAGGTGAACTCAGGCGAAGACGAGGTGAACTCTGACTCAGACG
AAGACGAGGTGAACTCAGACTCAGACTCAGACTCAGACGAGGAGGATATTTTTCCAGTCCCTGCACAAATCCTTGGTTTGGGAAAGGAAATGGAGAAACCAAACAGCGAAAACAAAACTGAAATGGAGAAATCGGGCAGTGAAAAAGGAGCTAAATCCTTCTGGTGGACTCCTAATTGTTCCAGCAAGCGGCGGGCCTGG
GCTGAAGCATTCAGTGCTTTGGATATTTCTTCGACGATGACGAGAAAAAAAAAACAACGTATATTTCTTCGACGATGACGAGAAAGAAGAGGAAGACGATGTGAACTCAGACGAAGACGAAGACGAGGACGATATTTTTCCAGTGCCTGCACAAATCAGTGGTTTAGGAATGGAAATGGAGAAACCAAGAAGTGAAAAAG
GACGATATTTTTCCAGTGCCTGCACAAATCCGAAACTGAAATGGAGAAATCGGGCCGAGAAAAAGGAGTGAAAAAGGAGCTAAATCGAAGGAAAGACTAGCTGAATCGAAGGAAAGACGAAAAAGGTTCGTTCTGGTGGACTCCTAATTGTTCCAGCAAGCGGCGGGCCTGAAGCAAGCGGCGGGCCTTCGCTGAAGCAT
TCAGTGCTTTGTAAATATGGCATTGCTATTACCTGCCAATCAAATCAATCATACTAATTGTTTTTCATTCGGAAACAAAATTGGTTTGCCTTATTCTCTTTTACTTATATCAAAATGGATCTTCTTAAATTCTTTTATGGGATAAACATATGAATCCTCATTTATATTTATAAG

>TRINITY_DN47556_c1_g3_i11 from sample A10 / Example unspliced isoform:

TGGCAGAGAAAGACGAAGTATATTTCTCCGAGGATGAGGATGACGAGAGAGAAGACGAGGTGAACTCAGGCGAAGACGAGGTGAACTCTGACTCAGACGAAGACGAGGTGAACTCAGACTCAGACTCAGACTCAGACGAGgtgaaccctaaaatatatacatctttttggctttccgaatttgtcattacatcttcttgg
ctttttggttttaaatggttacttcgttttatttgaacagGAGGATATTTTTCCAGTCCCTGCACAAATCCTTGGTTTGGGAAAGGAAATGGAGAAACCAAACAGCGAAAACAAAACTGAAATGGAGAAATCGGGCAGTGAAAAAGGAGCTAAATCCTTCTGGTGGACTCCTAATTGTTCCAGCAAGCGGCGGGCCTGGG
CTGAAGCATTCAGTGCTTTGGATATTTCTTCGACGATGACGAGAAAAAAAAAACAACGTATATTTCTTCGACGATGACGAGAAAGAAGAGGAAGACGATGTGAACTCAGACGAAGACGAAGACGAGGACGATATTTTTCCAGTGCCTGCACAAATCAGTGGTTTAGGAATGGAAATGGAGAAACCAAGAAGTGAAAAAGG
ACGATATTTTTCCAGTGCCTGCACAAATCCGAAACTGAAATGGAGAAATCGGGCCGAGAAAAAGGAGTGAAAAAGGAGCTAAATCGAAGGAAAGACTAGCTGAATCGAAGGAAAGACGAAAAAGGTTCGTTCTGGTGGACTCCTAATTGTTCCAGCAAGCGGCGGGCCTGAAGCAAGCGGCGGGCCTTCGCTGAAGCATT
CAGTGCTTTGTAAATATGGCATTGCTATTACCTGCCAATCAAATCAATCATACTAATTGTTTTTCATTCGGAAACAAAATTGGTTTGCCTTATTCTCTTTTACTTATATCAAAATGGATCTTCTTAAATTCTTTTATGGGATAAACATATGAATCCTCATTTATATTTATAAG

Intron 6 GTGAACTAAA ... ATTTTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.93	12.8	8	4
A2	34.57	13.8	8.6	6
A3	35.66	32.2	25.2	10.6
A4	29.79	12	7.2	3
A5	21.25	12	4.6	2
A6	16.93	178.2	43	29.6
A7	18.27	475.2	116.6	96
A8	16.23	532	90.6	115.6
A9	18.47	522.8	122	114.8
A10	18.53	391.2	105.4	72.6

>TRINITY_DN44550_c0_g2_i13 from sample A8 / Example spliced isoform

CTATATATCTTAGTGGATCCAGTCTTCCGCACATCAATACTCCCCGTTTCACTGCTGAGATTCACTTGTGTCAGCCCGGATTTTGTTTTGGTTGAAAGCTCCGAACAAATTGCCGTTTATTTGGTTGAAAGATCATAACAAATTGCAGTTTATTTGGTTGAAAGATCATAACAAATTGCATGTTGATATTATTATTGCTG
AAAATGACTTGGCTAGAGGAGTGGAATACATTTCTTGTCGTGGCAGAGAAAGACGAAGTATATTTCTCCGAGGATGACGAGAAAGAAGACGAAGACCAAGACGAGGTGAACTCAGACGAAGACGAAGACGGGGACGATATTTTTCCAGTGCCTGCACAAATCCATGTTTTCGAAAAGGAAATGGAGAAACCAAGCAGTGA
AAAAGAAACTGAAATAGAGAAATCGGGCCGAGAAAAAGGAGCTGAATCGAAGGAAAGACGAAAAAGGTCGTTCTGCTGGACTCCTAATTGTTCAAGCAAGCGGCGGGCCTTGGCTGAAGCATTCAGTGCTTTGTAAATATGGCATTGCTATTAACTGCCAATCAAATCAATCATACTAATATTTTAAAATGAAACAAAAC
ATTTCTGTCTTGTAATTCTCTTGTACTTTTATATAAAAAATTGTCTTTCATAAATATATGAATAGTCATTTAATAATGATCTTGTACTTTTATATAAAAAATTGTCTTTCATAAATATATGAATAGTCATTTAATAATGATCTTGTACTTTTATATAAAAAAT

>TRINITY_DN44550_c0_g2_i1 from sample A8 / Example unspliced isoform:

CTATATATCTTAGTGGATCCAGTCTTCCGCACATCAATACTCCCCGTTTCACTGCTGAGATTCACTTGTGTCAGCCCGGATTTTGTTTTGGTTGAAAGCTCCGAACAAATTGCCGTTTATTTGGTTGAAAGATCATAACAAATTGCAGTTTATTTGGTTGAAAGATCATAACAAATTGCATGTTGATATTATTATTGCTG
AAAATGACTTGGCTAGAGGAGTGGAATACATTTCTTGTCGTGGCAGAGAAAGACGAAGTATATTTCTCCGAGGATGACGAGAAAGAAGACGAAGACCAAGACGAGGTGAACTCAGACGAAGACGAAGACGGGgtgaactaaaaattccgctccctacccttaccctaaccctaaccctaaaatatatacatctttttggc
tttctgaattagtcattacatcttcttggctttctgaattagtcattacatcttcttggctttttggttttaaatggctacttcgttttattttaacagGACGATATTTTTCCAGTGCCTGCACAAATCCATGTTTTCGAAAAGGAAATGGAGAAACCAAGCAGTGAAAAAGAAACTGAAATAGAGAAATCGGGCCGAGA
AAAAGGAGCTGAATCGAAGGAAAGACGAAAAAGGTCGTTCTGCTGGACTCCTAATTGTTCAAGCAAGCGGCGGGCCTTGGCTGAAGCATTCAGTGCTTTGTAAATATGGCATTGCTATTAACTGCCAATCAAATCAATCATACTAATATTTTAAAATGAAACAAAACATTTCTGTCTTGTAATTCTCTTGTACTTTTATA
TAAAAAATTGTCTTTCATAAATATATGAATAGTCATTTAATAATGATCTTGTACTTTTATATAAAAAATTGTCTTTCATAAATATATGAATAGTCATTTAATAATGATCTTGTACTTTTATATAAAAAAT