sp|Q75LU8|CNBL3_ORYSJ ----> Calcineurin B-like protein 3

Intron 1 GTACGTTCTA ... GAAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.67	5	0.9	1
A2	51.25	2	0	2.1
A3	-1	0	0.9	0
A4	100	0	1	1
A5	66.67	1	0.9	2
A6	41.18	10	0	7
A7	69.06	3	5.5	6.8
A8	50	8	6.3	8
A9	53.85	6	8.3	7
A10	40	9	7.9	6

>TRINITY_DN26587_c0_g1_i2 from sample A6 / Example spliced isoform

CGTCATAATTACAAAATAAGATAAGAACACGGAAAGGAAGGACTTTTTGACATGCAATCTTACACACTACAGACGCGGAAGCCGGCCATTATTGATTTTACAAGGGCGTTGGATCCAGGGGAATCATGACCTGATCGATATGCTCATTTGTAATTTTCAGACATTCGTCTGCGAAATTGGAAGCCACTTCATTTTGATAA
CACCGGTATGGAGTATGGTCATGAATTGATACTATAATGGTGCAGTGCTTAGAGGAAATTGGCCATGTATTGGATCATCTATTGCCTTGTCTCGGTATAAAGTCTTTCATGCGACCTCGCCGCCTTAGAAACCCTGAGAAAGTGGCAAGTGAAACTGTTTTTAGTGTGAGTGAAGTAGAAGCATTATATGAGCTATTTAA
GAGGATTAGTAGTACTGTTATTGATGATGGTCTTATCAACAAGGAAGAGTTTCAACTGGCATTATTTAAAACCAATAAGAAAGAGAGCCTATTTGCGGATAGAGTTTTTGATTTGTTTGACACAAAGCATAAGAACGCACTAGGATTTGAAGAGTTTGCCCGTGCACTCTCGGTGTTTCACCCAAATGCTCCGATAGATG
AAAAAATTGATTTTTCCTTTGAACTCTATGACCTAAAGCAACAAGGCTTTATTGAGAGACAAGAGGTGAAGAGTATGGTAGTGGCCACGCTTGCTGAGTCGGGCATGAACCTTTCAGATGATGTCATCGAAAGTATAATTGACAAGACCTTTGAAGAGGCAGATACAAAACATGATGGAAAGATTGATAAAGAAGAATGG
AAGACCCTTGTTCTTCGTCACCCTCCACTGTTAAAAAATATGTCGCTTCAATATCTCAGGGATATTACGACTTCGTTTCCCAGCTTTGTCTTTTATTCACAAGTGGAAGATGCATGAGAAACTATTATATCATGCATTTTATTTAGCGAGGAGATCATGACCAATGTCACTTTTGTGAATTTTATACTTGCCAGATTCTT
GTAGGAGATAGCTATTTCCATTGTCTGTTTACCTGTCTTCGTTTTCTCTTTCCGAGTTCAATGTACAATGAAGTTTGGAGGTGACATATTACATTCTTTGTAATTTTTTGATTTGAGTAAAAATAGAAAAATTCCTCTGACAAAAGGACATTGAGAAAGGTGATGATTGACAATAAGAAATTATGTATGTGTTTAAGTTT
CAG

>TRINITY_DN26587_c0_g1_i1 from sample A6 / Example unspliced isoform:

GGGAAGATGCATGAgtacgttctacactcattagagaatagcaacttttgctggctgcaagttatggtaggcactattcaacagctgtctaaactcgaagtctatgtcgaaattgcagGAAACTATTATATCATGCATTTTATTTAGCGAGGAGATCATGACCAATGTCACTTTTGTGAATTTTATACTTGCCAGATTCT
TGTAGGAGATAGCTATTTCCATTGTCTGTTTACCTGTCTTCGTTTTCTCTTTCCGAGTTCAATGTACAATGAAGTTTGGAGGTGACATATTACATTCTTTGTAATTTTTTGATTTGAGTAAAAATAGAAAAATTCCTCTGACAAAAGGACATTGAGAAAGGTGATGATTGACAATAAGAAATTATGTATGTGTTTAAGTT
TCAG

Intron 2 GTTGATAATC ... TAATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75	2	3	6
A2	100	0	5	6
A3	100	0	0	1
A4	100	0	0	2
A5	40	3	0	2
A6	63.64	4	0	7
A7	64.52	11	5	20
A8	63.16	7	2	12
A9	68	8	3	17
A10	90.91	1	2	10

>TRINITY_DN34456_c0_g1_i3 from sample A9 / Example spliced isoform

GTGGACGTCATAATTACAAAATAAGATAAGAACACGGAAAGGAAGGACTTTTTGACATGCAATCTTACACACTACAGACGCGGAAGCCGGCCATTATTGGTTGATGATCGCTTTTAAATTTTGCCATCATTGGACATTCGTCTGCGAAATTGGAAGCCACTTCATTTTGATAACACCGGTATGGAGTATGGTCATGAATT
GATACTATAATGGTGCAGTGCTTAGAGGAAATTGGCCATGTATTGGATCATCTATTGCCTTGTCTCGGTATAAAGTCTTTCATGCGACCTCGCCGCCTTAGAAACCCTGAGAAAGTGGCAAGTGAAACTGTTTTTAGTGTGAGTGAAGTAGAAGCATTATATGAGCTATTTAAGAGGATTAGTAGTACTGTTATTGATGA
TGGTCTTATCAACAAGGAAGAGTTTCAACTGGCATTATTTAAAACCAATAAGAAAGAGAGCCTATTTGCGGATAGAGTTTTTGATTTGTTTGACACAAAGCATAAGAACGCACTAGGATTTGAAGAGTTTGCCCGTGCACTCTCGGTGTTTCACCCAAATGCTCCGATAGATGAAAAAATTGATTTTTCCTTTGAACTCT
ATGACCTAAAGCAACAAGGCTTTATTGAGAGACAAGAGGTGAAGAGTATGGTAGTGGCCACGCTTGCTGAGTCGGGCATGAACCTTTCAGATGATGTCATCGAAAGTATAATTGACAAGACCTTTGAAGAGGCAGATACAAAACATGATGGAAAGATTGATAAAGAAGAATGGAAGACCCTTGTTCTTCGTCACCCTCCA
CTGTTAAAAAATATGTCGCTTCAATATCTCAGGGATATTACGACTTCGTTTCCCAGCTTTGTCTTTTATTCACAAGTGGAAGATGCATGAGTACGTTCTACACTCATTAGAGAATAGCAACTTTTGCTGGCTGCAAGTTATGGTAGGCACTATTCAACAGCTGTCTAAACTCGAAGTCTATGTCGAAATTGCAGGAAACT
ATTATATCATGCATTTTATTTAGCGAGGAGATCATGACCAATGTCACTTTTGTGAATTTTATACTTGCCAGATTCTTGTAGGAGATAGCTATTTCCATTGTCTGTTTACCTGTCTTCGTTTTCTCTTTCCGAGTTCAATGTACAATGAAGTTTGGAGGTGACATATTACATTCTTTGTAATTTTTTGATTTGAGTAAAAA
TAGAAAAATTCCTCTGACAAAAGGACATTGAGAAAGGTGATGATTGACAATAAGAAATTATGTATGTGTTTAAGTTTCAGGGGTTCCATGCATTCAAGATTGTGTGATGTTAAATGTCTGCAGATTTGTTTTTCTAATGAAGGTAAGACATGCCCTTAGCATCTTTAGTGA

>TRINITY_DN34456_c0_g1_i6 from sample A9 / Example unspliced isoform:

GTGGACGTCATAATTACAAAATAAGATAAGAACACGGAAAGGAAGGACTTTTTGACATGCAATCTTACACACTACAGACGCGGAAGCCGGCCATTATTGGTTGATGATCGCTTTTAAATTTTGCCATCATTGGgttgataatcgcttttaaatttggctaatattgggttgatgatcgcctttattttttaattattaat
ttatttcagccatgattcgcgaaacagattttacaagggcgttggatccaggggaatcatgacctgatcgatatgctcatttgtaattttcagACATTCGTCTGCGAAATTGGAAGCCACTTCATTTTGATAACACCGGTATGGAGTATGGTCATGAATTGATACTATAATGGTGCAGTGCTTAGAGGAAATTGGCCATG
TATTGGATCATCTATTGCCTTGTCTCGGTATAAAGTCTTTCATGCGACCTCGCCGCCTTAGAAACCCTGAGAAAGTGGCAAGTGAAACTGTTTTTAGTGTGAGTGAAGTAGAAGCATTATATGAGCTATTTAAGAGGATTAGTAGTACTGTTATTGATGATGGTCTTATCAACAAGGAAGAGTTTCAACTGGCATTATTT
AAAACCAATAAGAAAGAGAGCCTATTTGCGGATAGAGTTTTTGATTTGTTTGACACAAAGCATAAGAACGCACTAGGATTTGAAGAGTTTGCCCGTGCACTCTCGGTGTTTCACCCAAATGCTCCGATAGATGAAAAAATTGATTTTTCCTTTGAACTCTATGACCTAAAGCAACAAGGCTTTATTGAGAGACAAGAGGT
GAAGAGTATGGTAGTGGCCACGCTTGCTGAGTCGGGCATGAACCTTTCAGATGATGTCATCGAAAGTATAATTGACAAGACCTTTGAAGAGGCAGATACAAAACATGATGGAAAGATTGATAAAGAAGAATGGAAGACCCTTGTTCTTCGTCACCCTCCACTGTTAAAAAATATGTCGCTTCAATATCTCAGGGATATTA
CGACTTCGTTTCCCAGCTTTGTCTTTTATTCACAAGTGGAAGATGCATGAGTACGTTCTACACTCATTAGAGAATAGCAACTTTTGCTGGCTGCAAGTTATGGTAGGCACTATTCAACAGCTGTCTAAACTCGAAGTCTATGTCGAAATTGCAGGAAACTATTATATCATGCATTTTATTTAGCGAGGAGATCATGACCA
ATGTCACTTTTGTGAATTTTATACTTGCCAGATTCTTGTAGGAGATAGCTATTTCCATTGTCTGTTTACCTGTCTTCGTTTTCTCTTTCCGAGTTCAATGTACAATGAAGTTTGGAGGTGACATATTACATTCTTTGTAATTTTTTGATTTGAGTAAAAATAGAAAAATTCCTCTGACAAAAGGACATTGAGAAAGGTGA
TGATTGACAATAAGAAATTATGTATGTGTTTAAGTTTCAGGGGTTCCATGCATTCAAGATTGTGTGATGTTAAATGTCTGCAGATTTGTTTTTCTAATGAAGGTAAGACATGCCCTTAGCATCTTTAGTGA

Intron 3 GTTGATGATC ... TTTATTTCAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	3	2	6
A2	100	0	3	4
A3	-1	0	0	0
A4	60	2	0	3
A5	-1	0	0	0
A6	-1	0	0	0
A7	100	0	1	6
A8	66.67	2	2	4
A9	80	1	2	4
A10	63.64	4	0	7

>TRINITY_DN34456_c0_g1_i2 from sample A9 / Example spliced isoform

GACGCGGAAGCCGGCCATTATTGCCATGATTCGCGAAACAGATTTTACAAGGGCGTTGGATCCAGGGGAATCATGACCTGATCGATATGCTCATTTGTAATTTTCAGACATTCGTCTGCGAAATTGGAAGCCACTTCATTTTGATAACACCGGTATGGAGTATGGTCATGAATTGATACTATAATGGTGCAGTGCTTAGA
GGAAATTGGCCATGTATTGGATCATCTATTGCCTTGTCTCGGTATAAAGTCTTTCATGCGACCTCGCCGCCTTAGAAACCCTGAGAAAGTGGCAAGTGAAACTGTTTTTAGTGTGAGTGAAGTAGAAGCATTATATGAGCTATTTAAGAGGATTAGTAGTACTGTTATTGATGATGGTCTTATCAACAAGGAAGAGTTTC
AACTGGCATTATTTAAAACCAATAAGAAAGAGAGCCTATTTGCGGATAGAGTTTTTGATTTGTTTGACACAAAGCATAAGAACGCACTAGGATTTGAAGAGTTTGCCCGTGCACTCTCGGTGTTTCACCCAAATGCTCCGATAGATGAAAAAATTGATTTTTCCTTTGAACTCTATGACCTAAAGCAACAAGGCTTTATT
GAGAGACAAGAGGTGAAGAGTATGGTAGTGGCCACGCTTGCTGAGTCGGGCATGAACCTTTCAGATGATGTCATCGAAAGTATAATTGACAAGACCTTTGAAGAGGCAGATACAAAACATGATGGAAAGATTGATAAAGAAGAATGGAAGACCCTTGTTCTTCGTCACCCTCCACTGTTAAAAAATATGTCGCTTCAATA
TCTCAGGGATATTACGACTTCGTTTCCCAGCTTTGTCTTTTATTCACAAGTGGAAGATGCATGAGTACGTTCTACACTCATTAGAGAATAGCAACTTTTGCTGGCTGCAAGTTATGGTAGGCACTATTCAACAGCTGTCTAAACTCGAAGTCTATGTCGAAATTGCAGGAAACTATTATATCATGCATTTTATTTAGCGA
GGAGATCATGACCAATGTCACTTTTGTGAATTTTATACTTGCCAGATTCTTGTAGGAGATAGCTATTTCCATTGTCTGTTTACCTGTCTTCGTTTTCTCTTTCCGAGTTCAATGTACAATGAAGTTTGGAGGTGACATATTACATTCTTTGTAATTTTTTGATTTGAGTAAAAATAGAAAAATTCCTCTGACAAAAGGAC
ATTGAGAAAGGTGATGATTGACAATAAGAAATTATGTATGTGTTTAAGTTTCAGGGGTTCCATGCATTCAAGATTGTGTGATGTTAAATGTCTGCAGATTTGTTTTTCTAATGAAGGTAAGACATGCCCTTAGCATCTTTAGTGA

>TRINITY_DN34456_c0_g1_i6 from sample A9 / Example unspliced isoform:

GTGGACGTCATAATTACAAAATAAGATAAGAACACGGAAAGGAAGGACTTTTTGACATGCAATCTTACACACTACAGACGCGGAAGCCGGCCATTATTGgttgatgatcgcttttaaattttgccatcattgggttgataatcgcttttaaatttggctaatattgggttgatgatcgcctttattttttaattattaat
ttatttcagCCATGATTCGCGAAACAGATTTTACAAGGGCGTTGGATCCAGGGGAATCATGACCTGATCGATATGCTCATTTGTAATTTTCAGACATTCGTCTGCGAAATTGGAAGCCACTTCATTTTGATAACACCGGTATGGAGTATGGTCATGAATTGATACTATAATGGTGCAGTGCTTAGAGGAAATTGGCCATG
TATTGGATCATCTATTGCCTTGTCTCGGTATAAAGTCTTTCATGCGACCTCGCCGCCTTAGAAACCCTGAGAAAGTGGCAAGTGAAACTGTTTTTAGTGTGAGTGAAGTAGAAGCATTATATGAGCTATTTAAGAGGATTAGTAGTACTGTTATTGATGATGGTCTTATCAACAAGGAAGAGTTTCAACTGGCATTATTT
AAAACCAATAAGAAAGAGAGCCTATTTGCGGATAGAGTTTTTGATTTGTTTGACACAAAGCATAAGAACGCACTAGGATTTGAAGAGTTTGCCCGTGCACTCTCGGTGTTTCACCCAAATGCTCCGATAGATGAAAAAATTGATTTTTCCTTTGAACTCTATGACCTAAAGCAACAAGGCTTTATTGAGAGACAAGAGGT
GAAGAGTATGGTAGTGGCCACGCTTGCTGAGTCGGGCATGAACCTTTCAGATGATGTCATCGAAAGTATAATTGACAAGACCTTTGAAGAGGCAGATACAAAACATGATGGAAAGATTGATAAAGAAGAATGGAAGACCCTTGTTCTTCGTCACCCTCCACTGTTAAAAAATATGTCGCTTCAATATCTCAGGGATATTA
CGACTTCGTTTCCCAGCTTTGTCTTTTATTCACAAGTGGAAGATGCATGAGTACGTTCTACACTCATTAGAGAATAGCAACTTTTGCTGGCTGCAAGTTATGGTAGGCACTATTCAACAGCTGTCTAAACTCGAAGTCTATGTCGAAATTGCAGGAAACTATTATATCATGCATTTTATTTAGCGAGGAGATCATGACCA
ATGTCACTTTTGTGAATTTTATACTTGCCAGATTCTTGTAGGAGATAGCTATTTCCATTGTCTGTTTACCTGTCTTCGTTTTCTCTTTCCGAGTTCAATGTACAATGAAGTTTGGAGGTGACATATTACATTCTTTGTAATTTTTTGATTTGAGTAAAAATAGAAAAATTCCTCTGACAAAAGGACATTGAGAAAGGTGA
TGATTGACAATAAGAAATTATGTATGTGTTTAAGTTTCAGGGGTTCCATGCATTCAAGATTGTGTGATGTTAAATGTCTGCAGATTTGTTTTTCTAATGAAGGTAAGACATGCCCTTAGCATCTTTAGTGA

Intron 4 GTTGATGATC ... CGCGAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70	3	0.8	7
A2	58.33	5	2.4	7
A3	-1	0	0	0
A4	100	0	0	3
A5	-1	0	0	0
A6	0	3	0	0
A7	50	10	1	10
A8	50	7	2	7
A9	41.67	7	1.4	5
A10	70	3	0	7

>TRINITY_DN35688_c0_g2_i2 from sample A8 / Example spliced isoform

CGTCAAAATTACAAAATAAGATAAGAACACGGAAAGGAAGGACTTTTTGACATGCAATCTTACACACTACAGACGCGGAAGCCGGCCATTATTGATTTTACAAGGGCGTTGGATCCAGGGGAATCATGACCTGATCGATATGCTCATTTGTAATTTTCAGACATTCGTCTGCGAAATTGGAAGCCACTTCATTTTGATAA
CACCGGTATGGAGTATGGTCATGAATTGATACTATAATGGTGCAGTGCTTAGAGGAAATTGGCCATGTATTGGATCATCTATTGCCTTGTCTCGGTATAAAGTCTTTCATGCGACCTCGCCGCCTTAGAAACCCTGAGAAAGTGGCAAGTGAAACTGTTTTTAGTGTGAGTGAAGTAGAAGCATTATATGAGCTATTTAA
GAGGATTAGTAGTACTGTTATTGATGATGGTCTTATCAACAAGGAAGAGTTTCAACTGGCATTATTTAAAACCAATAAGAAAGAGAGCCTATTTGCGGATAGAGTTTTTGATTTGTTTGACACAAAGCATAAGAACGCACTAGGATTTGAAGAGTTTGCCCGTGCACTCTCGGTGTTTCACCCAAATGCTCCGATAGATG
AAAAAATTGATTTTTCCTTTGAACTCTATGACCTAAAGCAACAAGGCTTTATTGAGAGACAAGAGGTGAAGAGTATGGTAGTGGCCACGCTTGCTGAGTCGGGCATGAACCTTTCAGATGATGTCATCGAAAGTATAATTGACAAGACCTTTGAAGAGGCAGATACAAAACATGATGGAAAGATTGATAAAGAAGAATGG
AAGACCCTTGTTCTTCGTCACCCTCCACTGTTAAAAAATATGTCGCTTCAATATCTCAGGGATATTACGACTTCGTTTCCCAGCTTTGTCTTTTATTCACAAGTGGAAGATGCATGAGAAACTATTATATCATGCATTTTATTTAGCGAGGAGATCATGACCAATGTCACTTTTGTGAATTTTATACTTGCCAGATTCTT
GTAGGAGATAGCTATTTCCATTGTCTGTTTACCTGTCTTCGTTTTCTCTTTCCGAGTTCAATGTACAATGAAGTTTGGAGGTGACATATTACATTCTTTGTAATTTTTTGATTTGAGTAAAAATAGAAAAATTCCTCTGACAAAAGGACATTGAGAAAGGTGATGATTGACAATAAGAAATTATGTATGTGTTTAAGTTT
CAGGGGTTCCATGCATTCAAGATTGTGTGATGTTAAATGTCTG

>TRINITY_DN35688_c0_g2_i5 from sample A8 / Example unspliced isoform:

GCCGGCCATTATTGgttgatgatcgcttttaaattttgccatcattgggttgataatcgcttttaaatttggctaatattgggttgatgatcgcctttattttttaattattaatttatttcagccatgattcgcgaaacagATTTTACAAGGGCGTTGGATCCAGGGGAATCATGACCTGATCGATATGCTCATTTGTA
ATTTTCAGACATTCGTCTGCGAAATTGGAAGCCACTTCATTTTGATAACACCGGTATGGAGTATGGTCATGAATTGATACTATAATGGTGCAGTGCTTAGAGGAAATTGGCCATGTATTGGATCATCTATTGCCTTGTCTCGGTATAAAGTCTTTCATGCGACCTCGCCGCCTTAGAAACCCTGAGAAAGTGGCAAGTGA
AACTGTTTTTAGTGTGAGTGAAGTAGAAGCATTATATGAGCTATTTAAGAGGATTAGTAGTACTGTTATTGATGATGGTCTTATCAACAAGGAAGAGTTTCAACTGGCATTATTTAAAACCAATAAGAAAGAGAGCCTATTTGCGGATAGAGTTTTTGATTTGTTTGACACAAAGCATAAGAACGCACTAGGATTTGAAG
AGTTTGCCCGTGCACTCTCGGTGTTTCACCCAAATGCTCCGATAGATGAAAAAATTGATTTTTCCTTTGAACTCTATGACCTAAAGCAACAAGGCTTTATTGAGAGACAAGAGGTGAAGAGTATGGTAGTGGCCACGCTTGCTGAGTCGGGCATGAACCTTTCAGATGATGTCATCGAAAGTATAATTGACAAGACCTTT
GAAGAGGCAGATACAAAACATGATGGAAAGATTGATAAAGAAGAATGGAAGACCCTTGTTCTTCGTCACCCTCCACTGTTAAAAAATATGTCGCTTCAATATCTCAGGGATATTACGACTTCGTTTCCCAGCTTTGTCTTTTATTCACAAGTGGAAGATGCATGAGTACGTTCTACACTCATTAGAGAATAGCAACTTTT
GCTGGCTGCAAGTTATGGTAGGCACTATTCAACAGCTGTCTAAACTCGAAGTCTATGTCGAAATTGCAGGAAACTATTATATCATGCATTTTATTTAGCGAGGAGATCATGACCAATGTCACTTTTGTGAATTTTATACTTGCCAGATTCTTGTAGGAGATAGCTATTTCCATTGTCTGTTTACCTGTCTTCGTTTTCTC
TTTCCGAGTTCAATGTACAATGAAGTTTGGAGGTGACATATTACATTCTTTGTAATTTTTTGATTTGAGTAAAAATAGAAAAATTCCTCTGACAAAAGGACATTGAGAAAGGTGATGATTGACAATAAGAAATTATGTATGTGTTTAAGTTTCAGGGGTTCCATGCATTCAAGATTGTGTGATGTTAAATGTCTG