NA ----> NA

Intron 1 GTATGAATTT ... CCGATCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	0	1	0	0
A5	-1	0	0	0
A6	11.76	15	2	0
A7	18.75	26	6	0
A8	15	34	6	0
A9	19.51	33	8	2
A10	6.25	15	1	0

>TRINITY_DN35326_c0_g1_i3 from sample A9 / Example spliced isoform

ATAAACCTTGACTCTGATTGTTCGCTTATTACTGGAACATCTGCATCACTTGTAGATAAAAACAGCCACATCTGCAAGTGATGTAGATGTGCTAGCCTTAGTTCATTTGCTAACTTTGTCTTCTCTTCTCAATATTCTTTAGTAACGGCATCTCAATATTCTTTTGTAACGGATCTTTTTCTCTTCTCAATATCTTTAGT
AACGGCATCTTTTTGGTGACCTAGGCGAGGTAAGCGAGGAGAACGGTACGGGAAATACCAAAGTTAAAACCAGCGCAGAGGGTGGGGTTACAGCTGATTCTGAATTTCTTGTGGTAGTCTTGTATCCTTAGATTATCAGGTAGCAACAATGGCAGATGATGGTGCCAGTGCTATTTGCAGCATGGAAGTAAGTCTTCTTA
TAAACAATTAAAAAACCGGACGGGTCCTGTATGCGGAGGCAGGTAAGGACTTCGTGGATCTCCTCTTGGCTTTTCTTCTGCTGCCGACTGGTGCCATCATCCGACTTCTGCGCGAACATGGTACGGCGACTTCCGAGTCAGTGGGAAGCATAAGTAACGTGTACAACAGTGTCCACAATCTGAAAGCGGCCTTTATGAAA
ACTGATAAATCCATTTTGCTGGAACCGCAACTAGAATCCACCCCCTTCAACAGTTTCCCACAACTTCAATATTCTACGACTCCTCGCGACTACTATGTATGCCCCATCGCTAGCAACGGTAACGGAAAGCACCAAATTAGTACCCGAGCATGCCATAAATGCACATGTGGCCGAGAAATAAAAACTGCGTTGGTGTTCGT
TATTCCGCTCTCGTCCCCGTCTTCTTCTAAGAATTCCGGGAGCTCTGAAGGGTTCGTGAAAGAGACTGTTAGATTTATAATCACCGATGATTTGAATATCCTTCCAATTTCTACCGTTACCATTATTACTCTCTTGAATCGGCTACAAGTGAAAGACCTAAGCGAATTGAAGGAAATGACCGTAACAGTCGGTCCTGACA
AGGTTTTGGAACTGCTGGGCGCGTCTATGGCTTCAAAGACCGCTTTAAACGACGTTTTCTCTCCGAAATCAGCATCCGTCGCTCAAGAGGAGTAGAAGATAGATCGATGACTCGTTTTTCGGAAGTTAGGGTTTGATTGAACAATTGTTATCATGAAACGCTTTCAGTTCAATGGCTGTTGTAGCCTGTACTTGATATTT
TGATAGTGTCAAAATATATTTGTTGGTATATTAATCGTGATATATTATTTGTCTATGCAATGCTTTTAGTTGTCCAAGCGAGTAATGGAATCCTTTCACATGGAACTGTAAAGTATATCATATTATAAGTGGGATTTGAGAACTTCAACAATATGGTGATCACACCAAATCTCTTTTGACTTCCACATTAGCTTGTGTCA
TCC

>TRINITY_DN35326_c0_g1_i1 from sample A9 / Example unspliced isoform:

ATAAACCTTGACTCTGATTGTTCGCTTATTACTGGAACATCTGCATCACTTGTAGATAAAAACAGCCACATCTGCAAGTGATGTAGATGTGCTAGCCTTAGTTCATTTGCTAACTTTGTCTTCTCTTCTCAATATTCTTTAGTAACGGCATCTCAATATTCTTTTGTAACGGATCTTTTTCTCTTCTCAATATCTTTAGT
AACGGCATCTTTTTGGTGACCTAGGCGAGGTAAGCGAGGAGAACGGTACGGGAAATACCAAAGTTAAAACCAGCGCAGAGGGTGGGGTTACAGCTGATTCTGAATTTCTTGTGGTAGTCTTGTATCCTTAGATTATCAGGTAGCAACAATGGCAGATGATGGTGCCAGTGCTATTTGCAGCATGGAAGTAAGTCTTCTTA
TAAACAATTAAAAAACCGGACGGGTCCTGTATGCGGAGGCAGGTAAGGACTTCGTGGATCTCCTCTTGGCTTTTCTTCTGCTGCCGACTGGTGCCATCATCCGACTTCTGCGCGAACATGGTACGGCGACTTCCGAGTCAGTGGGAAGCATAAGTAACGTGTACAACAGTGTCCACAATCTGAAAGCGGCCTTTATGAAA
ACTGATAAATCCATTTTGCTGGAACCGCAACTAGAATCCACCCCCTTCAACAGTTTCCCACAACTTCAATATTCTACGACTCCTCGCGACTACTATGTATGCCCCATCGCTAGCAACGGTAACGGAAAGCACCAAATTAGTACCCGAGCATGCCATAAATGCACATGTGGCCGAGAAATAAAAACTGCGTTGGTGTTCGT
TATTCCGCTCTCGTCCCCGTCTTCTTCTAAGAATTCCGGGAGCTCTGAAGGGTTCGTGAAAGAGACTGTTAGATTTATAATCACCGATGATTTGAATATCCTTCCAATTTCTACCGTTACCATTATTACTCTCTTGAATCGGCTACAAGTGAAAGACCTAAGCGAATTGAAGGAAATGACCGTAACAGTCGGTCCTGACA
AGgtatgaatttttattcaattaatcggcagcctctatttagtgctgcaacttgttgataaattgaatttgtaggctttattttacgaaacgtaatgccgatcacagGTTTTGGAACTGCTGG

Intron 2 GTTTTGGAAC ... GGCTTCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	100	0	1	1
A5	-1	0	0	0
A6	57.75	15	15	26
A7	69.86	11	26	25
A8	72.92	13	34	36
A9	68.93	16	33	38
A10	73.33	6	15	18

>TRINITY_DN39339_c0_g1_i3 from sample A8 / Example spliced isoform

AGGTAAGCGAGGAGAACGGTACGGGAAATACCAAAGTTAAAACCAGCGCAGAGGGTGGGGTTACAGCTGATTCTGAATTTCTTGTGGTAGTCTTGTATCCTTAGATTATCAGGTAGCAACAATGGCAGATGATGGTGCCAGTGCTATTTGCAGCATGGAAGTAAGTCTTCTTATAAACAATTAAAAAACCGGACGGGTCC
TGTATGCGGAGGCAGGTAAGGACTTCGTGGATCTCCTCTTGGCTTTTCTTCTGCTGCCGACTGGTGCCATCATCCGACTTCTGCGCGAACATGGTACGGCGACTTCCGAGTCAGTGGGAAGCATAAGTAACGTGTACAACAGTGTCCACAATCTGAAAGCGGCCTTTATGAAAACTGATAAATCCATTTTGCTGGAACCG
CAACTAGAATCCACCCCCTTCAACAGTTTCCCACAACTTCAATATTCTACGACTCCTCGCGACTACTATGTATGCCCCATCGCTAGCAACGGTAACGGAAAGCACCAAATTAGTACCCGAGCATGCCATAAATGCACATGTGGCCGAGAAATAAAAACTGCGTTGGTGTTCGTTATTCCGCTCTCGTCCCCGTCTTCTTC
TAAGAATTCCGGGAGCTCTGAAGGGTTCGTGAAAGAGACTGTTAGATTTATAATCACCGATGATTTGAATATCCTTCCAATTTCTACCGTTACCATTATTACTCTCTTGAATCGGCTACAAGTGAAAGACCTAAGCGAATTGAAGGAAATGACCGTAACAGTCGGTCCTGACAAGACCGCTTTAAACGACGTTTTCTCTC
CGAAATCAGCATCCGTCGCTCAAGAGGAGTAGAAGATAGATCGATGACTCGTTTTTCGGAAGTTAGGGTTTGATTGAACAATTGTTATCATGAAACGCTTTCAGTTCAATGGCTGTTGTAGCCTGTACTTGATATTTTGATAGTGTCAAAATATATTTGTTGGTATATTAATCGTGATATATTATTTGTCTATGCAATGC
TTTTAGTTGTCCAAGCGAGTAATGGAATCCTTTCACATGGAACTGTAAAGTATATCATATTATAAGTGGGATTTGAGAACTTCAACAATATGGTGATCACACCAAATCTCTTTTGAC

>TRINITY_DN39339_c0_g1_i2 from sample A8 / Example unspliced isoform:

AGGTAAGCGAGGAGAACGGTACGGGAAATACCAAAGTTAAAACCAGCGCAGAGGGTGGGGTTACAGCTGATTCTGAATTTCTTGTGGTAGTCTTGTATCCTTAGATTATCAGGTAGCAACAATGGCAGATGATGGTGCCAGTGCTATTTGCAGCATGGAAGTAAGTCTTCTTATAAACAATTAAAAAACCGGACGGGTCC
TGTATGCGGAGGCAGGTAAGGACTTCGTGGATCTCCTCTTGGCTTTTCTTCTGCTGCCGACTGGTGCCATCATCCGACTTCTGCGCGAACATGGTACGGCGACTTCCGAGTCAGTGGGAAGCATAAGTAACGTGTACAACAGTGTCCACAATCTGAAAGCGGCCTTTATGAAAACTGATAAATCCATTTTGCTGGAACCG
CAACTAGAATCCACCCCCTTCAACAGTTTCCCACAACTTCAATATTCTACGACTCCTCGCGACTACTATGTATGCCCCATCGCTAGCAACGGTAACGGAAAGCACCAAATTAGTACCCGAGCATGCCATAAATGCACATGTGGCCGAGAAATAAAAACTGCGTTGGTGTTCGTTATTCCGCTCTCGTCCCCGTCTTCTTC
TAAGAATTCCGGGAGCTCTGAAGGGTTCGTGAAAGAGACTGTTAGATTTATAATCACCGATGATTTGAATATCCTTCCAATTTCTACCGTTACCATTATTACTCTCTTGAATCGGCTACAAGTGAAAGACCTAAGCGAATTGAAGGAAATGACCGTAACAGTCGGTCCTGACAAGgttttggaactgctgggcgcgtcta
tggcttcaaagACCGCTTTAAACGACGTTTTCTCTCCGAAATCAGCATCCGTCGCTCAAGAGGAGTAGAAGATAGATCGATGACTCGTTTTTCGGAAGTTAGGGTTTGATTGAACAATTGTTATCATGAAACGCTTTCAGTTCAATGGCTGTTGTAGCCTGTACTTGATATTTTGATAGTGTCAAAATATATTTGTTGGT
ATATTAATCGTGATATATTATTTGTCTATGCAATGCTTTTAGTTGTCCAAGCGAGTAATGGAATCCTTTCACATGGAACTGTAAAGTATATCATATTATAAGTGGGATTTGAGAACTTCAACAATATGGTGATCACACCAAATCTCTTTTGAC