sp|Q8LGG8|USPAL_ARATH ----> Universal stress protein A-like protein

Intron 1 GTATAACAGT ... TATATATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.84	22	70	48
A2	88	3	34	10
A3	64.52	11	26	14
A4	69.23	8	22	14
A5	81.82	2	14	4
A6	79.02	15	85	28
A7	79.12	19	107	37
A8	76.47	26	118	51
A9	72.38	25	91	40
A10	72.63	26	111	27

>TRINITY_DN44273_c0_g8_i4 from sample A6 / Example spliced isoform

AGACAGTAAACCCGCGGAAATGCAAGGCCGGAATCTTCTTCGATTCGAACGCACTTGAACACGCTGGTTTTCCCATTTATTTGTGCCTACAACTTCCCATCCTCGCCATAACCTCTTTATTCGCTGCATAGAGCAAAACAATTTAGTGGGTGTGAAGATCGGTGTAAGAAGAGACAAAATATTGTGCATTGGAGTCAATG
GAGGAGCATAATATATCAGGCAGAGTCATATTGGTGGCCATAGACAAGAGCAAGGAGAGCTGTGATGCTCTCCAATGGGCTCTTAACAACCTGTTTACATCCCAGGATAGAATCATTCTCATTCATGCTCAGCGCCTTCCAGTTTCCTCTTTCTCAACTGGAAACCCAGGTTGATTTCTTCAGGCATTTTTCTCAGGCTG
TTATGGAGATTTGTAGGATGTAATTCACTGTAAAATCATACAAGGCTCTTGAGTTTATTAAGTGAAAGGATTTTAATCTTTTTCGACACATACAACAGTAGAATGGGCGTGTTCTGTTCGTTCATCTCAGAGCTTGTGATGGCATTTCATCTTGAAGTGTTTTATTCTTTTTTGGGATTGTTATAGAGGCCGAGTTCTGA
TTGCATTTCGGCGTGGCCAGGTTTCATGATGCCAGTAGATGTGCTCAAGATATTGGAAAACGATATCCAGAAGTCTACAGAGAAGATTTTCGCCATAGCAACTGAAATATGCAAAGCCAAAAATGTAAGTTAGGTATACTCATATTGTGATTGGAATTAAAGAATTTGGTGTGAACTAGATCGGAGGGTTGATTCAAAAT
TTAATATAGTTTTATTTTCTTTAATCATTGGCTGACTGTATTGAATCTTTCCTAAACATGCTTTCATTACAAATTGCGCGGGGAGAAGGAAGGCCCCTGGAGCTCCATCTTGGCGCGGGGCTCCATCAACACTCTGTTTGTGGGGGAAATCTGCAGGGTAGTTTTTCTCGTTTTGAGGATGGTATTCAGGGTAAACCAGA
TTCTTTAGGATTCAAAGTCAATATAACAATATGGTTATTTAAGATCTCAGAATCAACAATATGCTTATTTAAGAGCTCAAAATCTTGAGTGAGTAGGGCAACAATCAGTCCTAATTACTCAAACTACACCCAAGTCATGATATTCATCCTGTCATTGTAAGATCTGAGATATTAGTACACTCTTGCTAATACCAATTCGA
AGACCACAATACACAGTCTTGAGCACGCCCATGGCATTCTGCAGCTTTTCCGGCTTCTTTTCCTCTGTAATTCCATAATGGAAAACCTTAGAAGATTTTTCCTTCTACTGTCCCAGGTGACTCCGGAAACACATGCTCACAATGGAGATGCCAGGGAGGTCATCTGCGATGCTGCCAAAAAGTATAATTCTGACATGTTG
GTGATTGGTAGTCATGCATACGGGGTTCTTAAGAGTTCTTGGCAGTGTGAGTGACTATTGTGTCCACCATGTACAGTGCCCTGTTGTTGTTGTGAAGCCCCAGAAGGTCGAGGCTCATTGATTTTCATTTGCCTCTTGCCACCTAAGTGC

>TRINITY_DN44273_c0_g8_i3 from sample A6 / Example unspliced isoform:

AGACAGTAAACCCGCGGAAATGCAAGGCCGGAATCTTCTTCGATTCGAACGCACTTGAACACGCTGGTTTTCCCATTTATTTGTGCCTACAACTTCCCATCCTCGCCATAACCTCTTTATTCGCTGCATAGAGCAAAACAATTTAGTGGGTGTGAAGATCGGTGTAAGAAGAGACAAAATATTGTGCATTGGAGTCAATG
GAGGAGCATAATATATCAGGCAGAGTCATATTGGTGGCCATAGACAAGAGCAAGGAGAGCTGTGATGCTCTCCAATGGGCTCTTAACAACCTGTTTACATCCCAGGATAGAATCATTCTCATTCATGCTCAGCGCCTTCCAGTTTCCTCTTTCTCAACTGGAAACCCAGGTTGATTTCTTCAGGCATTTTTCTCAGGCTG
TTATGGAGATTTGTAGGATGTAATTCACTGTAAAATCATACAAGGCTCTTGAGTTTATTAAGTGAAAGGATTTTAATCTTTTTCGACACATACAACAGTAGAATGGGCGTGTTCTGTTCGTTCATCTCAGAGCTTGTGATGGCATTTCATCTTGAAGTGTTTTATTCTTTTTTGGGATTGTTATAGAGGCCGAGTTCTGA
TTGCATTTCGGCGTGGCCAGGTTTCATGATGCCAGTAGATGTGCTCAAGATATTGGAAAACGATATCCAGAAGTCTACAGAGAAGATTTTCGCCATAGCAACTGAAATATGCAAAGCCAAAAATGTAAGTTAGGTATACTCATATTGTGATTGGAATTAAAGAATTTGGTGTGAACTAGATCGGAGGGTTGATTCAAAAT
TTAATATAGTTTTATTTTCTTTAATCATTGGCTGACTGTATTGAATCTTTCCTAAACATGCTTTCATTACAAATTGCGCGGGGAGAAGGAAGGCCCCTGGAGCTCCATCTTGGCGCGGGGCTCCATCAACACTCTGTTTGTGgtataacagttcactgaatctacatggttgattgggtttatataatatagacattcgg
agagatctatttgaattttggtttttgtccatcaagaagatgaagatgttgttatcaggtttatgcgaagatgaggatcgttttgaatgtccatggatagtttaactctcttccaagtaattttcatcctcctaaacagatagataatctatagtaatttaacctccacaaatgccaactgctctttagattgccaagct
ggagttcacataagcttgagattaaatagatccattttcataggcttcttaattgagtgttgaaatccttcaattcatttgagaaaaaagtgcaattagaagccagtgcgaatttcagaatgtgagtggctaatattgagccttgatcttgatcttttctgtttcaaaatcaaaacccatctttcagcaagatactggtt
caaattcaatttacggttaagtgcctttatgcttgttttggaattttttgatacatttaacttcaagcagattctatccattaaggtcttatctcattctagttaggactgcaaactttaataggaaggtccttcaatggatcagctccatattatcacttttatcaattttgcaaaatgtaattgtgaggtttattgcc
atcactctattggttatgatattcatattttttttcttcttggaatatatatcagGGGGAAATCTGCAGGGTAGTTTTTCTCGTTTTGAGGATGGTATTCAGGGTAAACCAGATTCTTTAGGATTCAAAGTCAATATAACAATATGGTTATTTAAGATCTCAGAATCAACAATATGCTTATTTAAGAGCTCAAAATCTTG
AGTGAGTAGGGCAACAATCAGTCCTAATTACTCAAACTACACCCAAGTCATGATATTCATCCTGTCATTGTAAGATCTGAGATATTAGTACACTCTTGCTAATACCAATTCGAAGACCACAATACACAGTCTTGAGCACGCCCATGGCATTCTGCAGCTTTTCCGGCTTCTTTTCCTCTGTAATTCCATAATGGAAAACC
TTAGAAGATTTTTCCTTCTACTGTCCCAGGTGACTCCGGAAACACATGCTCACAATGGAGATGCCAGGGAGGTCATCTGCGATGCTGCCAAAAAGTATAATTCTGACATGTTGGTGATTGGTAGTCATGCATACGGGGTTCTTAAGAGTTCTTGGCAGTGTGAGTGACTATTGTGTCCACCATGTACAGTGCCCTGTTGT
TGTTGTGAAGCCCCAGAAGGTCGAGGCTCATTGATTTTCATTTGCCTCTTGCCACCTAAGTGC

Intron 2 GTTGATTTCT ... GCGTGGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.34	165	56	38
A2	29.63	152	64	20
A3	34.4	164	86	24
A4	33.51	125	63	24
A5	28.33	86	34	9
A6	25.4	884	301	41
A7	28.19	851	334	34
A8	24.16	741	236	47
A9	26.16	779	276	47
A10	27.95	794	308	65

>TRINITY_DN44273_c0_g8_i2 from sample A6 / Example spliced isoform

AGACAGTAAACCCGCGGAAATGCAAGGCCGGAATCTTCTTCGATTCGAACGCACTTGAACACGCTGGTTTTCCCATTTATTTGTGCCTACAACTTCCCATCCTCGCCATAACCTCTTTATTCGCTGCATAGAGCAAAACAATTTAGTGGGTGTGAAGATCGGTGTAAGAAGAGACAAAATATTGTGCATTGGAGTCAATG
GAGGAGCATAATATATCAGGCAGAGTCATATTGGTGGCCATAGACAAGAGCAAGGAGAGCTGTGATGCTCTCCAATGGGCTCTTAACAACCTGTTTACATCCCAGGATAGAATCATTCTCATTCATGCTCAGCGCCTTCCAGTTTCCTCTTTCTCAACTGGAAACCCAGGTTTCATGATGCCAGTAGATGTGCTCAAGAT
ATTGGAAAACGATATCCAGAAGTCTACAGAGAAGATTTTCGCCATAGCAACTGAAATATGCAAAGCCAAAAATGTGACTCCGGAAACACATGCTCACAATGGAGATGCCAGGGAGGTCATCTGCGATGCTGCCAAAAAGTATAATTCTGACATGTTGGTGATTGGTAGTCATGCATACGGGGTTCTTAAGAGGGCAGTTC
TTGGCAGTGTGAGTGACTATTGTGTCCACCATGTACAGTGCCCTGTTGTTGTTGTGAAGCCCCAGAAGGTCGAGGCTCATTGATTTTCATTTGCCTCTTGCCACCTAAGTGC

>TRINITY_DN44273_c0_g8_i4 from sample A6 / Example unspliced isoform:

AGACAGTAAACCCGCGGAAATGCAAGGCCGGAATCTTCTTCGATTCGAACGCACTTGAACACGCTGGTTTTCCCATTTATTTGTGCCTACAACTTCCCATCCTCGCCATAACCTCTTTATTCGCTGCATAGAGCAAAACAATTTAGTGGGTGTGAAGATCGGTGTAAGAAGAGACAAAATATTGTGCATTGGAGTCAATG
GAGGAGCATAATATATCAGGCAGAGTCATATTGGTGGCCATAGACAAGAGCAAGGAGAGCTGTGATGCTCTCCAATGGGCTCTTAACAACCTGTTTACATCCCAGGATAGAATCATTCTCATTCATGCTCAGCGCCTTCCAGTTTCCTCTTTCTCAACTGGAAACCCAGgttgatttcttcaggcatttttctcaggctg
ttatggagatttgtaggatgtaattcactgtaaaatcatacaaggctcttgagtttattaagtgaaaggattttaatctttttcgacacatacaacagtagaatgggcgtgttctgttcgttcatctcagagcttgtgatggcatttcatcttgaagtgttttattcttttttgggattgttatagaggccgagttctga
ttgcatttcggcgtggccagGTTTCATGATGCCAGTAGATGTGCTCAAGATATTGGAAAACGATATCCAGAAGTCTACAGAGAAGATTTTCGCCATAGCAACTGAAATATGCAAAGCCAAAAATGTAAGTTAGGTATACTCATATTGTGATTGGAATTAAAGAATTTGGTGTGAACTAGATCGGAGGGTTGATTCAAAAT
TTAATATAGTTTTATTTTCTTTAATCATTGGCTGACTGTATTGAATCTTTCCTAAACATGCTTTCATTACAAATTGCGCGGGGAGAAGGAAGGCCCCTGGAGCTCCATCTTGGCGCGGGGCTCCATCAACACTCTGTTTGTGGGGGAAATCTGCAGGGTAGTTTTTCTCGTTTTGAGGATGGTATTCAGGGTAAACCAGA
TTCTTTAGGATTCAAAGTCAATATAACAATATGGTTATTTAAGATCTCAGAATCAACAATATGCTTATTTAAGAGCTCAAAATCTTGAGTGAGTAGGGCAACAATCAGTCCTAATTACTCAAACTACACCCAAGTCATGATATTCATCCTGTCATTGTAAGATCTGAGATATTAGTACACTCTTGCTAATACCAATTCGA
AGACCACAATACACAGTCTTGAGCACGCCCATGGCATTCTGCAGCTTTTCCGGCTTCTTTTCCTCTGTAATTCCATAATGGAAAACCTTAGAAGATTTTTCCTTCTACTGTCCCAGGTGACTCCGGAAACACATGCTCACAATGGAGATGCCAGGGAGGTCATCTGCGATGCTGCCAAAAAGTATAATTCTGACATGTTG
GTGATTGGTAGTCATGCATACGGGGTTCTTAAGAGTTCTTGGCAGTGTGAGTGACTATTGTGTCCACCATGTACAGTGCCCTGTTGTTGTTGTGAAGCCCCAGAAGGTCGAGGCTCATTGATTTTCATTTGCCTCTTGCCACCTAAGTGC