NA ----> NA

Intron 1 GTACGTTAAT ... ATTTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.86	52.9	21.1	12
A2	19.34	48.9	15.2	11
A3	28.96	47.7	14.5	19
A4	27.87	25.3	11.9	9
A5	30.42	28.1	6.7	12
A6	26.42	400.4	98.3	140
A7	25.88	411.4	87.2	140
A8	28.38	457.9	120.1	176
A9	27.47	423.1	98.7	156
A10	25.94	283.5	54.9	97

>TRINITY_DN116614_c1_g1_i2 from sample A1 / Example spliced isoform

ATTTATATCAAATCGCCAAATCTATCAACACAACATTTCCAAACGATGGTAGGAGGTGGGAAGTGACCTGTGGAGTCTTTAAGGTTTATTTCTGGAAAATCCAGCAAGATCCCTCTCCACGTGATCATTAAACCAGGCTATGTAGTGGGCATATTCCTCGCTTATACATTTGACACCACAAGCGTTTCCCCTCAGAAACT
CCGGCGCAAGACCAGTCACTGAACGCAACAAAGAAGTCTACACCTATGCGCGCAGGTTCGATTCCCTGGCACATCCAGCTTTGGCGTGAACACAAACAGAGACTCTGAAATTGGGGAAGAAGAAGTGAGTAAATCTGGCAAACTACGGGGAATTGAGGGCCAGGTCGTCCTCGAGACAAGCCCCCACAGACGACGAAGAA
GATTGCAGGCCCTGGCAGAGGAGGCTTAGCATGACAGTCACACGGGGATCCATGTTCCGATTGCTTCTCGTGCTTCTTCTTCTCGGTGCCTTCATTACGGCTTGCCTCACTCTGCCGATGGAAAAGACGTTAAAAGACTTCTTGGTCTGGATTGATAAGAATTTAGGCCATTGGGGGCCTCTTGTGTTGGCTGTGGCATA
TATTCCATTGACAGTGCTTGCAGTTCCAGCATCCATCCTTACACTTGGAGGTGGCTACCTGTTTGGGCTGACTACTGGCTTTATTGCGGATTCCATTGGATCTACTGCTGGTGCGACAGCTGCATTCCTCGTTGGTAGAACGATTGGAAGGTCTTATGTAGTTGCCAAATTGAAGGACTATCCTCAGTTTCAAGCTGTCG
CTATAGCAGTTCAACGTTCTGGTTTCAAGGTTCAATCTCTGTGTGTATCATTTTGCTTCTTAGGCTTGTTCCACTACTTCCTTTCAACATGCTAAATTATCTTCTTTCAGTGACGCCTGTCAGTATAGGGGAGTACATTCTTGCATCATGGATTGGTACGATGCCAATAACTTTTGCTTTTGTGTATGTTGGAACGACAA
TAAAGGATCTCTCTGATATAACTCATGGCTGGAATGAAATTTCAACAACTCGTTGGATATTGCTCAGTATTGGTTCGATAGCCTCTGTTGTTCTAATGGTTTTGGTGACTAAAGTGGCAAGGAAGTCACTCAACAAGGCCCTTGAGGAAAATGACAGCAAACTGGATTGCATTTTGGTACCACCAGACTTACCAGTTGTA
AATGAACCCCGCTTGGATCTTTTACATCCACTGATTATTAAAGTAGACCCTATTGTGAACAATCATGATCACTAGTCATTTGATTAATGTAAGCTGTGTTAATGATCTTTGAAATGGAAGCATATGTAAAAAGCAAATATTGTTGTTATGGTCCATATGTCTATAATAGTTGTGTATATCATGCAACTGTGGAAATTTTG
TTTCCATTTCTCCAATCCTGTATATTAATGATAAAAGTCATGAAAAGGCA

>TRINITY_DN116614_c1_g1_i6 from sample A1 / Example unspliced isoform:

ATTTATATCAAATCGCCAAATCTATCAACACAACATTTCCAAACGATGGTAGGAGGTGGGAAGTGACCTGTGGAGTCTTTAAGGTTTATTTCTGGAAAATCCAGCAAGATCCCTCTCCACGTGATCATTAAACCAGGCTATGTAGTGGGCATATTCCTCGCTTATACATTTGACACCACAAGCGTTTCCCCTCAGAAACT
CCGGCGCAAGACCAGTCACTGAACGCAACAAAGAAGTCTACACCTATGCGCGCAGGTTCGATTCCCTGGCACATCCAGCTTTGGCGTGAACACAAACAGAGACTCTGAAATTGGGGAAGAAGAAGTGAGTAAATCTGGCAAACTACGGGGAATTGAGGGCCAGGTCGTCCTCGAGACAAGCCCCCACAGACGACGAAGAA
GATTGCAGGCCCTGGCAGAGGAGGCTTAGCATGACAGTCACACGGGGATCCATGTTCCGATTGCTTCTCGTGCTTCTTCTTCTCGGTGCCTTCATTACGGCTTGCCTCACTCTGCCGATGGAAAAGACGTTAAAAGACTTCTTGGTCTGGATTGATAAGAATTTAGGCCATTGGGGGCCTCTTGTGTTGGCTGTGGCATA
TATTCCATTGACAGTGCTTGCAGTTCCAGCATCCATCCTTACACTTGGAGGTGGCTACCTGTTTGGGCTGACTACTGGCTTTATTGCGGATTCCATTGGATCTACTGCTGGTGCGACAGCTGCATTCCTCGTTGGTAGAACGATTGGAAGGTCTTATGTAGTTGCCAAATTGAAGGACTATCCTCAGTTTCAAGCTGTCG
CTATAGCAGTTCAACGTTCTGGTTTCAAGCCAATAACTTTTGCTTTTGTGTATGTTGGAACGACAATAAAGGATCTCTCTGATATAACTCATGGCTGGAATGAAATTTCAACAACTCGTTGGATATTGCTCAGTATTGGTTCGATAGCCTCTGgtacgttaatatttttgacttgcagtatattcttcccatatggttta
cctttgaagcattttctggttaacatctccaattgttaagtggctgccttgtgtatctgatgactatatataaaagtaggttgtgccttgatgcttgtttttcatcatctacttgtaggaattttactgatttaaattgcatttttgtagTTGTTCTAATGGTTTTGGTGACTAAAGTGGCAAGGAAGTCACTCAACAAG
GCCCTTGAGGAAAATGACAGCAAACTGGATTGCATTTTGGTACCACCAGACTTACCAGTTGTAAATGAACCCCGCTTGGATCTTTTACATCCACTGATTATTAAAGTAGACCCTATTGTGAACAATCATGATCACTAGTCATTTGATTAATGTAAGCTGTGTTAATGATCTTTGAAATGGAAGCATATGTAAAAAGCAAA
TATTGTTGTTATGGTCCATATGTCTATAATAGTTGTGTATATCATGCAACTGTGGAAATTTTGTTTCCATTTCTCCAATCCTGTATATTAATGATAAAAGTCATGAAAAGGCA

Intron 2 GTTCAATCTC ... CTTCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	78.7	18	65	68
A2	67.3	26	53	54
A3	72.95	28	63	88
A4	69.3	35	76	82
A5	65.63	11	18	24
A6	69.37	136	300	316
A7	71.43	122	309	301
A8	69.53	149	300	380
A9	64.25	165	307	286
A10	70.25	94	220	224

>TRINITY_DN40351_c0_g1_i2 from sample A8 / Example spliced isoform

CATTTCCAAACGATGGTAGGAGGTGGGAAGTGACCTGTGGAGTCTTTAAGGTTTATTTCTGGAAAATCCAGCAAGATCCCTCTCCACGTGATCATTAAACCAGGCTATGTAGTGGGCATATTCCACGCTTATACATTTGACACCACAAGCGTTTCCCCTCAGAAACTCCGGCGCAAGACCAGTCACTGAACGCAACAAAG
AAGTCCACACCTATGCGCGCAGGTTCGATTCCCTGGCACATCCAGCCTTAGCGTGAACACAAACAGAGACTCTGAAATTGGGGAAGAAGAAGTGAGTAAATCTGGCAAACTACGGGGAATTGAGGGCCAGGTCGTCCTCGAGACAAGCCCCCACAGACGACGAAGAAGATTGCAGGCCCTGGCAGAGGAGGCTTAGCATG
ACAGTCACACGGGGATCCATGTTCCGATTGCTTCTCGTGCTTCTTCTTCTCGGTGCCTTCGCTACGGCTTGCCTCACTCTGCCGATGGAAAAGACGTTAAAAGACTTCTTGGTCTGGATTGATAAGAATTTAGGCCATTGGGGGCCTCTTGTGTTGGCTGTGGCATATATTCCATTGACAGTGCTTGCAGTTCCAGCATC
CATCCTTACACTTGGAGGTGGCTACCTGTTTGGGCTGACTACTGGCTTTATTGCGGATTCCATTGGATCTACTGCTGGTGCGACAGCTGCATTCCTCGTTGGTAGAACGATTGGAAGGTCTTATGTAGTTGCCAAATTGAAGGACTATCCTCAGTTTCAAGCTGTCGCTATAGCAGTTCAACGTTCTGGTTTCAAGTGAC
GCCTGTCAGTATAGGGGAGTACATTCTTGCATCATGGATTGGTACGATGCCAATAACTTTTGCTTTTGTGTATGTTGGAACGACAATAAAGGATCTCTCTGATATAACTCATGGCTGGAATGAAATTTCAACAACTCGTTGGAT

>TRINITY_DN40351_c0_g1_i4 from sample A8 / Example unspliced isoform:

CATTTCCAAACGATGGTAGGAGGTGGGAAGTGACCTGTGGAGTCTTTAAGGTTTATTTCTGGAAAATCCAGCAAGATCCCTCTCCACGTGATCATTAAACCAGGCTATGTAGTGGGCATATTCCACGCTTATACATTTGACACCACAAGCGTTTCCCCTCAGAAACTCCGGCGCAAGACCAGTCACTGAACGCAACAAAG
AAGTCCACACCTATGCGCGCAGGTTCGATTCCCTGGCACATCCAGCCTTAGCGTGAACACAAACAGAGACTCTGAAATTGGGGAAGAAGAAGTGAGTAAATCTGGCAAACTACGGGGAATTGAGGGCCAGGTCGTCCTCGAGACAAGCCCCCACAGACGACGAAGAAGATTGCAGGCCCTGGCAGAGGAGGCTTAGCATG
ACAGTCACACGGGGATCCATGTTCCGATTGCTTCTCGTGCTTCTTCTTCTCGGTGCCTTCGCTACGGCTTGCCTCACTCTGCCGATGGAAAAGACGTTAAAAGACTTCTTGGTCTGGATTGATAAGAATTTAGGCCATTGGGGGCCTCTTGTGTTGGCTGTGGCATATATTCCATTGACAGTGCTTGCAGTTCCAGCATC
CATCCTTACACTTGGAGGTGGCTACCTGTTTGGGCTGACTACTGGCTTTATTGCGGATTCCATTGGATCTACTGCTGGTGCGACAGCTGCATTCCTCGTTGGTAGAACGATTGGAAGGTCTTATGTAGTTGCCAAATTGAAGGACTATCCTCAGTTTCAAGCTGTCGCTATAGCAGTTCAACGTTCTGGTTTCAAGgttc
aatctctgtgtgtatcattttgcttcttaggcttgttccactacttcctttcaacatgctaaattatcttctttcagTGACGCCTGTCAGTATAGGGGAGTACATTCTTGCATCATGGATTGGTACGATGCCAATAACTTTTGCTTTTGTGTATGTTGGAACGACAATAAAGGATCTCTCTGATATAACTCATGGCTGGA
ATGAAATTTCAACAACTCGTTGGATATTGCTCAGTATTGGTTCGATAGCCTCTGTTGTTCTAATGGTTTTGGTGACTAAAGTGGCAAGGAAGTCACTCAACAAGGCCCTTGAGGAAAATGACAGCAAACTGGATTGCATTTTGGTACCACCAGACTTACCAGTTGTAAATGAACCCCGCTTGGATCTTTTACATCCACTG
ATTATTAAAGTAGACCCTATTGTGAACAATCATGATCACTAGTCATTTGATTAATGTAAGCTGTGTTAATGATCTTTGAAATGGAAGCATATGTAAAAAGCAAATATTGTTGTTATGGTCCATATGTCTATAATAGTTGTGTATATCATGCAACTGTGGAAATTTTGTTTCCATTTCTCCAATCCTGTATATTAATGATA
AAAGTCATGAAAAGGCAACATGGCGAGCAGTCTTCCTGTTTATTGTGGCCTGGTTTACCCTTATCAAATTCTAAATCCTGCTGATTGATAGATAATATAGGGCATCCTGTATCAAGAAACAAAATCTCATCTCCGTGGATTTGCTGCTTGTATTTTTTAGAAACTAAAAATCCCGATGTGTAATGCATTCCGGGTCCACT
TTCCTTGTGGATAAACTAATTTAAAACTAGATATCTTTGGGAAAGAACTGAAGGCTGATCATTATAATTGAATAAAGTTAAGGAGATGGATTTTTGAAAGAAAATGCTTTAAAG

Intron 3 GTGTATCATT ... CTTCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.67	6	64	68
A2	83.33	11	56	54
A3	94.94	4	62	88
A4	86.59	12	73	82
A5	83.33	4	16	24
A6	90.22	33	293	316
A7	88.68	39	310	301
A8	90.21	37	302	380
A9	87.77	39	274	286
A10	89.09	27	217	224

>TRINITY_DN43996_c0_g2_i6 from sample A10 / Example spliced isoform

ATCAACACAACATTTCCAAACGATGGTAGGAGGTGGGAAGTGACCTGTGGAGTCTTTAAGGTTTATTTCTGGAAAATCCAGCAAGATCCCTCTCCACGTGATCATTAAACCAGGCTATGTAGTGGGCATATTCCTCGCTTATACATTTGACACCACAAGCGTTTCCCCTCAGAAACTCCGGCGCAAGACCAGTCACTGAA
CGCAACAAAGAAGTCCACACCTATGCGCGCAGGTTCGATTCCCTGGCACATCCAGCCTTAGCGTGAACACAAACAGAGACTCTGAAATTGGGGAAGAAGAAGTGAGTAAATCTGGCAAACTACGGGGAATTGAGGGCCAGGTCGTCCTCGAGACAAGCCCCCACAGACGACGAAGAAGATTGCAGGCCCTGGCAGAGGAG
GCTTAGCATGACAGTCACACGGGGATCCATGTTCCGATTGCTTCTCGTGCTTCTTCTTCTCGGTGCCTTCGCTACGGCTTGCCTCACTCTGCCGATGGAAAAGACGTTAAAAGACTTCTTGGTCTGGATTGATAAGAATTTAGGCCATTGGGGGCCTCTTGTGTTGGCTGTGGCATATATTCCATTGACAGTGCTTGCAG
TTCCAGCATCCATCCTTACACTTGGAGGTGGCTACCTGTTTGGGCTGACTACTGGCTTTATTGCGGATTCCATTGGATCTACTGCTGGTGCGACAGCTGCATTCCTCGTTGGTAGAACGATTGGAAGGTCTTATGTAGTTGCCAAATTGAAGGACTATCCTCAGTTTCAAGCTGTCGCTATAGCAGTTCAACGTTCTGGT
TTCAAGGTTCAATCTCTGTTGACGCCTGTCAGTATAGGGGAGTACATTCTTGCATCATGGATTGGTACGATG

>TRINITY_DN43996_c0_g2_i4 from sample A10 / Example unspliced isoform:

ATCAACACAACATTTCCAAACGATGGTAGGAGGTGGGAAGTGACCTGTGGAGTCTTTAAGGTTTATTTCTGGAAAATCCAGCAAGATCCCTCTCCACGTGATCATTAAACCAGGCTATGTAGTGGGCATATTCCTCGCTTATACATTTGACACCACAAGCGTTTCCCCTCAGAAACTCCGGCGCAAGACCAGTCACTGAA
CGCAACAAAGAAGTCCACACCTATGCGCGCAGGTTCGATTCCCTGGCACATCCAGCCTTAGCGTGAACACAAACAGAGACTCTGAAATTGGGGAAGAAGAAGTGAGTAAATCTGGCAAACTACGGGGAATTGAGGGCCAGGTCGTCCTCGAGACAAGCCCCCACAGACGACGAAGAAGATTGCAGGCCCTGGCAGAGGAG
GCTTAGCATGACAGTCACACGGGGATCCATGTTCCGATTGCTTCTCGTGCTTCTTCTTCTCGGTGCCTTCGCTACGGCTTGCCTCACTCTGCCGATGGAAAAGACGTTAAAAGACTTCTTGGTCTGGATTGATAAGAATTTAGGCCATTGGGGGCCTCTTGTGTTGGCTGTGGCATATATTCCATTGACAGTGCTTGCAG
TTCCAGCATCCATCCTTACACTTGGAGGTGGCTACCTGTTTGGGCTGACTACTGGCTTTATTGCGGATTCCATTGGATCTACTGCTGGTGCGACAGCTGCATTCCTCGTTGGTAGAACGATTGGAAGGTCTTATGTAGTTGCCAAATTGAAGGACTATCCTCAGTTTCAAGCTGTCGCTATAGCAGTTCAACGTTCTGGT
TTCAAGGTTCAATCTCTGTgtgtatcattttgcttcttaggcttgttccactacttcctttcaacatgctaaattatcttctttcagTGACGCCTGTCAGTATAGGGGAGTACATTCTTGCATCATGGATTGGTACGATGCCAATAACTTTTGCTTTTGTGTATGTTGGAACGACAATAAAGGATCTCTCTGATATAACT
CATGGCTGGAATGAAATTTCAACAACTCGTTGGATATTGCTCAGTATTGGTTCGATAGCCTCTGTTGTTCTAATGGTTTTGGTGACTAAAGTGGCAAGGAAGTCACTCAACAAGGCCCTTGAGGAAAATGACAGCAAACTGGATTGCATTTTGGTACCACCAGACTTACCAGTTGTAAATGAACCCCGCTTGGATCTTTT
ACATCCACTGATTATTAAAGTAGACCCTATTGTGAACAATCATGATCACTAGTCATTTGATTAATGTAAGCTGTGTTAATGATCTTTGAAATGGAAGCATATGTAAAAAGCAAATATTGTTGTTATGGTCCATATGTCTATAATAGTTGTGTATATCATGCAACTGTGGAAATTTTGTTTCCATTTCTCCAATCCTGTAT
ATTAATGATAAAAGTCATGAAAAGGCAACATGGCGAGCAGTCTTCCTGTTTATTGTGGCCTGGTTTACCCTTATCAAATTCTAAATCCTGCTGATTGATAGATAATATAGGGCATCCTGTATCAACAAACAAAATCTCATCTCCGT