sp|O22611|DRM1_PEA ----> Dormancy-associated protein 1

Intron 1 GTAACTATCT ... TTTGTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.29	24	69.4	25
A2	61.11	59	122.4	63
A3	57.64	32	56.1	31
A4	45.13	31	27	24
A5	53.81	39	48.9	42
A6	38.03	60.3	34	40
A7	56.53	24	31.4	31
A8	50	29	32	26
A9	48.63	78	72.7	75
A10	44.6	26	25.9	16

>TRINITY_DN117256_c3_g2_i2 from sample A2 / Example spliced isoform

GAAAAGGCTACTTGCAATTTTGCAATTTTGACTGGTTCGAAAACGACACTCAGTCTGAAAATTAGGTTGGAGTCACATAAAAGTTCCGCAATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGATAGTGGCCTCAACCGGCTTCGTAACAATTCCAATTTCAATATTAGCCACCGACCAGATAG
TAACAGTGATATCTAATTATTGGATGTTGCAATCGCATGATGTTGACGAGATAGGAGAAGGGAGTGAAGGAAATGCTGTGAATGTGGATGGAAAGCTATCTAAATTCAATATTCAACCCTCGTTATCCATGGAAAGCTCTACGAGTCCGCCATCTTCACCAACTGCAGCCTCTTCTCCTTCATCTACTCCGCGAGATCAG
GAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAACACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGCTTTACAGTGAAGACACGAAATCGAAAAGGGGCTGAGAACATTTTTCTGGAGGGATGTTACAAGTCCAAACAGTAATGTACAGTGTTCG
AATGTGTTTCTGAGGATATAATTCCTTTTAGTGTGGATTGTAATTACCAAGTAGTTGTATTGTGTGATAAGAGGTGTAACCCATGAAGTCGGATGTTGAACTATCGTACACTGTCCAATTGAAGGGTATGAACATTTCAATTTGGTTCATATTTCAATTTGGCTTTCACAATATTCCATATAAGGAATTAAGAAGGTGAT
ATAGCAGTGGTTTTATAATTTTTGTTTTGTAGAGGGGAGCAACATGCAAAAATTCTCAAATTGCAATGTCTGAATGCTTAACTAATACTTGTAATATCCAATATCTAGTGAATGTATTTAGGGCTTCCCTAAACCATGTATTGAAGACTCTTTCAGGATCTGATTGTATGAAATAGTGTATATATAATTTTTTGGTTTAA
TTTATAATGGCTATAAAGATTTTGTAAGGGCATGTATTTATAGTACTAGATAGTATAAATGTGTTGTTTATAGTAATTATCGTATAGATATTTTTTTTCAATCTTATAAGTTATAAACCAAATTATAAACCATGATCATTAATATTTTAAGTATGTTGGGCC

>TRINITY_DN117256_c3_g2_i1 from sample A2 / Example unspliced isoform:

GAAAAGGCTACTTGCAATTTTGCAATTTTGACTGGTTCGAAAACGACACTCAGTCTGAAAATTAGGTTGGAGTCACATAAAAGTTCCGCAATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGATAGTGGCCTCAACCGGCTTCGTAACAATTCCAATTTCAATATTAGCCACCGACCAGgtaa
ctatctttaatttattattggttactagtatatttacatggataggtgtttatatgttaaaatttggggtggattactatctatgtttttactagaatgaataggtgtttataagttagaatttaggtggattaccatctatgtttttactagattaaagaagattgaagaaggattagacaaatgtattattgattgat
tttatagtggtagttcaaaattccaaataaactattataaaaatatatatatataaaataatgtgtatggataactcctaactcatgcatggataaggtagtcacaaacagtagctgaggcatagataacttgtgattacaactaagcatggagaaccagaatcagataacttgttcttgttcatggcacttgtaaccta
ttgatgttcaccaaccacaaattaagtgatttatgctaattattatttttgttgtagATAGTAACAGTGATATCTAATTATTGGATGTTGCAATCGCATGATGTTGACGAGACAGGAGAAGGGAGTGAAGGAAATGCTGTGAATGTGGATGGAAAGCTATCTAAATTCAATATTCAACCCTCGTTATCCATGGAAAGCTC
TACGAGTCCGCCATCTTCACCAACTGCAGCCTCTTCTCCTTCATCTACTCCGCGAGATCAGGAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAACACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGCTTTACAGTGAAGACACGAAATCGAAAA
GGGGCTGAGAACATTTTTCTGGAGGGATGTTACAAGTCCAAACAGTAATGTACAGTGTTCGAATGTGTTTCTGAGGATATAATTCCTTTTAGTGTGGATTGTAATTACCAAGTAGTTGTATTGTGTGATAAGAGGTGTAACCCATGAAGTCGGATGTTGAACTATCGTACACTGTCCAATTGAAGGGTATGAACATTTCA
ATTTGGTTCATATTTCAATTTGGCTTTCACAATATTCCATATAAGGAATTAAGAAGGTGATATAGCAGTGGTTTTATAATTTTTGTTTTGTAGAGGGGAGCAACATGCAAAAATTCTCAAATTGCAATGTCTGAATGCTTAACTAATACTTGTAATATCCAATATCTAGTGAATGTATTTAGGGCTTCCCTAAACCATGT
ATTGAAGACTCTTTCAGGATCTGATTGTATGAAATAGTGTATATATAATTTTTTGGTTTAATTTATAATGGCTATAAAGATTTTGTAAGGGCATGTATTTATAGTACTAGATAGTATAAATGTGTTGTTTATAGTAATTATCGTATAGATATTTTTTTTCAATCTTATAAGTTATAAACCAAATTATAAACCATGATCAT
TAATATTTTAAGTATGTTGGGCC

Intron 2 GTAAGCAGCT ... TCACATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.43	16	0	7
A2	36.36	7	0	4
A3	33.33	10	1	5
A4	40	3	1	2
A5	28.57	10	0	4
A6	11.76	45	0	6
A7	14.63	35	0	6
A8	31.03	20	0	9
A9	24.44	34	1	11
A10	11.11	32	0	4

>TRINITY_DN41857_c0_g1_i6 from sample A6 / Example spliced isoform

TAGAAATCCATTAGTCACAGGTTTGTAAAGAAAGACAGACTCAGACTGAGACTCAGTTTTGGTAGGCGCAGACGAGAATTAGTTGAGCCGATCATGTTGGATAAGATATGGGATGACACTCTTGGCGGTCCACAGCCAGACAACGGCCTCAGCAGACTGCGCAAGAATTCTACTTTGAATGCTACTTATGGATCAGCAGC
AGGAAAAATGCAGGTGAGATCCCTTGATGTCGATGATATGAGTGAGGGAAATCGTGTAATTCGAAAGCCGCCAAAGTTCAATATTCAACGCTCGCTATCCATGGAAAGTAGCGCTTCTACTAGTCCTCCATCTTCACCAACTGCGGCCTCTTCACCATCAGCTTCCTCTACTCCACGAGATCGGGAGAACGTATGGAGAA
GCGTGTTCAACCCAGGGAGTAACCCCAACACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGG

>TRINITY_DN41857_c0_g1_i7 from sample A6 / Example unspliced isoform:

TTTGAATGCTACTTATGGATCAGgtaagcagctctgatccatctagatcgcagagtaattagcctgccagatctattctgtcagtagttttgttgtttttcttgttcgcagcagcagcaacagcaagcagtatataattattgggtattgtgatcacatgcagCAGCAGGAAAAATGCAGGTGAGATCCCTTGATGTCGA
TGATATGAGTGAGGGAAATCGTGTAATTCGAAAGCCGCCAAAGTTCAATATTCAACGCTCGCTATCCATGGAAAGTAGCGCTTCTACTAGTCCTCCATCTTCACCAACTGCGGCCTCTTCACCATCAGCTTCCTCTACTCCACGAGATCGGGAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAACACCA
ACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGG

Intron 3 GTGAGTCGTT ... AATTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.16	181	27	48
A2	16.79	280	46	67
A3	10.88	217	24	29
A4	12.26	136	10	28
A5	14.29	195	13	52
A6	10.76	539	55	75
A7	11.8	329	35	53
A8	9.23	236	18	30
A9	11.32	521	54	79
A10	13.69	268	25	60

>TRINITY_DN125309_c0_g2_i2 from sample A1 / Example spliced isoform

AAAAGGCTGCTTGCAATTTTGACAGGTTCGAAAACAAGACTCAGCGACTGAAAATTAGCTTGGAGTCACTAAAAAGTTGAGCGATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGACAGCGGCCTCAGAAGGCTTCGCAACAATTCCACTTTCAACGCTGGCCACGGAGCAAGCCGAGAAGGG
AGTCAGGGAAATGCTGTGAATGGAAAGCCCTCTAAATTCAATATTCAACGCTCGCTATCCATGGAAATCTCTACGAGTCCGCCATCTTCACCAACTGCAGTCACTTCCCCTTCATCTACTCCGCGAGATCGTGAGAACATATGGAGAAGCGTGTTCGACCCAGGCAGTAACCCCAATACCAACTCAATTGGATCTCAAAG
ATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGTTTTACAGTGGAGACACGAAATCGAAATGGCGCTGAGAACATATTTTCCTGGATGGATGGTACAAGTCCAAACAATAATGTACAATGTTGGCGTGTGTTTCTGAGGATAGATTTGCTTTAGTGTGGGATTGTAATTACCAAGTATTTGCAGTATGTG
ATAAGAAGTGTAAGCCATGAAGTCGGACGTTGAAATATCATACACTATCCTTTGTATTTTGGTGCTATGTACATACGAATATGAACATTACAATTTGGTTTCCACAATATTCCATAAGGGAGTTAAAAAACTGATATAGCAGTGGTTTTATGTTTTCTATCTTATAGAGGGTAGCAGTATGCAAAAATTCTACACTTCCA
AGTGATGAAATGTGC

>TRINITY_DN125309_c0_g2_i1 from sample A1 / Example unspliced isoform:

AAAAGGCTGCTTGCAATTTTGACAGGTTCGAAAACAAGACTCAGCGACTGAAAATTAGCTTGGAGTCACTAAAAAGTTGAGCGATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGACAGCGGCCTCAGAAGGCTTCGCAACAATTCCACTTTCAACGCTGGCCACGGAGCAAGCCGAGAAGGG
AGTCAGGGAAATGCTGTGAATGGAAAGCCCTCTAAATTCAATATTCAACGCTCGCTATCCATGGAAATCTCTACGAGTCCGCCATCTTCACCAACTGCAGTCACTTCCCCTTCATgtgagtcgttcccaactgaacgttttgacctacttttctctgcttgagtagctttcatatgcatccatgcatatgcattatcttt
tgtgaagtttggtatcttaatggatagaaactagatgaaatcataaatggtgtatgaatgaattgtacagCTACTCCGCGAGATCGTGAGAACATATGGAGAAGCGTGTTCGACCCAGGCAGTAACCCCAATACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGTT
TTACAGTGGAGACACGAAATCGAAATGGCGCTGAGAACATATTTTCCTGGATGGATGGTACAAGTCCAAACAATAATGTACAATGTTGGCGTGTGTTTCTGAGGATAGATTTGCTTTAGTGTGGGATTGTAATTACCAAGTATTTGCAGTATGTGATAAGAAGTGTAAGCCATGAAGTCGGACGTTGAAATATCATACAC
TATCCTTTGTATTTTGGTGCTATGTACATACGAATATGAACATTACAATTTGGTTTCCACAATATTCCATAAGGGAGTTAAAAAACTGATATAGCAGTGGTTTTATGTTTTCTATCTTATAGAGGGTAGCAGTATGCAAAAATTCTACACTTCCAAGTGATGAAATGTGC

Intron 4 GTATCATTTA ... TTCTAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.12	179.1	93.6	67
A2	28	188.1	75.9	69.5
A3	24.6	159.7	58	45
A4	26.99	132.7	60.2	37
A5	29.74	124.3	61.9	43
A6	12.26	936.4	161.1	91
A7	15.52	395.3	87	49
A8	17.83	407.6	90.8	76.5
A9	17.98	611.5	144.3	115
A10	17.96	285.6	90.4	27

>TRINITY_DN125309_c0_g2_i7 from sample A1 / Example spliced isoform

TGAAAAGGCTACTTGCAATTTTGCAATTTTGACTGGTTCGAAAACGACACTCAGTCTGAAAATTAGGTTGGAGTCACATAAAAGTTCCGCAATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGATAGTGGCCTCAACCGGCTTCGTAACAATTCCAATTTCAATATTAGCCACCGACCAGGTA
ACTATCTTTAATTTATTATTGGTTACTAGTATATTTACATGGATAGGTGTTTATATGTTAAAATTTGGGGTGGATTACTATCTATGTTTTTACTAGAATGAATAGGTGTTTATAAGTTAGAATTTAGGTGGATTACCATCTATGTTTTTACTAGATTAAAGAAGATTGAAGAAGGATTAGACAAATGTATTATTGATTGA
TTTTATAGTGGTAGTTCAAAATTCCAAATAAACTATTATAAAAATATATATATATAAAATAATGTGTATGGATAACTCCTAACTCATGCATGGATAAGGTAGTCACAAACAGTAGCTGAGGCATAGATAACTTGTGATTACAACTAAGCATGGAGAACCAGAATCAGATAACTTGTTCTTGTTCATGGCACTTGTAACCT
ATTGATGTTCACCAACCACAAATTAAGTGATTTATGCTAATTATTATTTTTGTTGTAGATAGTAACAGTGATATCTAATTATTGGATGTTGCAATCGCATGATGTTGACGAGATAGGAGAAGGGAGTGAAGGAAATGCTGTGAATGTGGATGGAAAGCTATCTAAATTCAATATTCAACCCTCGTTATCCATGGAAAGCT
CTACGAGTCCGCCATCTTCACCAACTGCAGCCTCTTCTCCTTCATCTACTCCGCGAGATCAGGAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAATACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGCTTTACAGTGAAGACACGAAATCGAAA
AGGGGCTGAGAACATTTTTCTGGAGGGATGTTACAAGTCCAAACAGTAATGTACAGTGTTCGAATGTGTTTCTGAGGATATAATTCCTTTTAGTGTGGATTGTAATTACCAAGTAGTTGTATTGTGTGATAAGAGGTGTAACCCATGAAGTCGGATGTTGAACTATCGTACACTGTCCAATTGAAGGGTATGAACATTTC
AATTTGGTTCATATTTCAATTTGGCTTTCACAATATTCCATATAAGGAATTAAGAAGGTGATATAGCAGTGGTTTTATAATTTTTGTTTTGTAGAGGGGAGCAACATGCAAAAATTCTCAAATTGCAATGTCTGAATGCTTAACTAATACTTGTAATATCCAATATCTAGTGAATGTATTTAGGGCTTCCCTAAACCATG
TATTGAAGACTCTTTCAGGATCTGATTGTATGAAATAGTGTATATA

>TRINITY_DN125309_c0_g2_i11 from sample A1 / Example unspliced isoform:

TGAAAAGGCTACTTGCAATTTTGCAATTTTGACTGGTTCGAAAACGACACTCAGTCTGAAAATTAGGTTGGAGTCACATAAAAGTTCCGCAATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGATAGTGGCCTCAACCGGCTTCGTAACAATTCCAATTTCAATATTAGCCACCGACCAGGTA
ACTATCTTTAATTTATTATTGGTTACTAGTATATTTACATGGATAGGTGTTTATATGTTAAAATTTGGGGTGGATTACTATCTATGTTTTTACTAGAATGAATAGGTGTTTATAAGTTAGAATTTAGGTGGATTACCATCTATGTTTTTACTAGATTAAAGAAGATTGAAGAAGGATTAGACAAATGTATTATTGATTGA
TTTTATAGTGGTAGTTCAAAATTCCAAATAAACTATTATAAAAATATATATATATAAAATAATGTGTATGGATAACTCCTAACTCATGCATGGATAAGGTAGTCACAAACAGTAGCTGAGGCATAGATAACTTGTGATTACAACTAAGCATGGAGAACCAGAATCAGATAACTTGTTCTTGTTCATGGCACTTGTAACCT
ATTGATGTTCACCAACCACAAATTAAGTGATTTATGCTAATTATTATTTTTGTTGTAGATAGTAACAGTGATATCTAATTATTGGATGTTGCAATCGCATGATGTTGACGAGATAGGAGAAGGGAGTGAAGGAAATGCTGTGAATGTGGATGGAAAGCTATCTAAATTCAATATTCAACCCTCGTTATCCATGGAAAGCT
CTACGAGTCCGCCATCTTCACCAACTGCAGCCTCTTCTCCTTCATCTACTCCGCGAGATCAGGAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAATACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGgtatcatttactcctctcctactccatt
aaacaagttgcttttgtacagtttgactgagacacgcagttaagaataagtgtgttttttttatttttaaagtgagttcgattgtaaagaagtaaggttttttacagttctgtaaactaacaaaccaaatccatgtaccatattggggtctcctaacccaaaaagtacaaaattacaagtcaatttgaagtcattattac
cctatttttactttgttgttggtaccattaaatccttctgtccctgtttttgggatggcaacttctaagcagGCTTTACAGTGAAGACACGAAATCGAAAAGGGGCTGAGAACATTTTTCTGGAGGGATGTTACAAGTCCAAACAGTAATGTACAGTGTTCGAATGTGTTTCTGAGGATATAATTCCTTTTAGTGTGGAT
TGTAATTACCAAGTAGTTGTATTGTGTGATAAGAGGTGTAACCCATGAAGTCGGATGTTGAACTATCGTACACTGTCCAATTGAAGGGTATGAACATTTCAATTTGGTTCATATTTCAATTTGGCTTTCACAATATTCCATATAAGGAATTAAGAAGGTGATATAGCAGTGGTTTTATAATTTTTGTTTTGTAGAGGGGA
GCAACATGCAAAAATTCTCAAATTGCAATGTCTGAATGCTTAACTAATACTTGTAATATCCAATATCTAGTGAATGTATTTAGGGCTTCCCTAAACCATGTATTGAAGACTCTTTCAGGATCTGATTGTATGAAATAGTGTATATA

Intron 5 GTAACTATCT ... GACGAGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40.55	90	69.8	53
A2	46.81	137.6	122.8	119.3
A3	41.57	74	56.7	48.7
A4	38.57	56	27	43.3
A5	37.18	93	48.9	61.2
A6	29.52	142	34	85
A7	31.68	109.5	31.8	69.8
A8	31.31	98	32	57.3
A9	35.67	183	72.9	130
A10	30.13	78	25.9	41.3

>TRINITY_DN125309_c0_g2_i6 from sample A1 / Example spliced isoform

TGAAAAGGCTACTTGCAATTTTGCAATTTTGACTGGTTCGAAAACGACACTCAGTCTGAAAATTAGGTTGGAGTCACATAAAAGTTCCGCAATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGATAGTGGCCTCAACCGGCTTCGTAACAATTCCAATTTCAATATTAGCCACCGACCAGGAG
AAGGGAGTGAAGGAAATGCTGTGAATGTGGATGGAAAGCTATCTAAATTCAATATTCAACCCTCGTTATCCATGGAAAGCTCTACGAGTCCGCCATCTTCACCAACTGCAGCCTCTTCTCCTTCATCTACTCCGCGAGATCAGGAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAATACCAACTCAATT
GGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGTATCATTTACTCCTCTCCTACTCCATTAAACAAGTTGCTTTTGTACAGTTTGACTGAGACACGCAGTTAAGAATAAGTGTGTTTTTTTTATTTTTAAAGTGAGTTCGATTGTAAAGAAGTAAGGTTTTTTACAGTTCTGTAAACTA
ACAAACCAAATCCATGTACCATATTGGGGTCTCCTAACCCAAAAAGTACAAAATTACAAGTCAATTTGAAGTCATTATTACCCTATTTTTACTTTGTTGTTGGTACCATTAAATCCTTCTGTCCCTGTTTTTGGGATGGCAACTTCTAAGCAGGCTTTACAGTGAAGACACGAAATCGAAAAGGGGCTGAGAACATTTTT
CTGGAGGGATGTTACAAGTCCAAACAGTAATGTACAGTGTTCGAATGTGTTTCTGAGGATATAATTCCTTTTAGTGTGGATTGTAATTACCAAGTAGTTGTATTGTGTGATAAGAGGTGTAACCCATGAAGTCGGATGTTGAACTATCGTACACTGTCCAATTGAAGGGTATGAACATTTCAATTTGGTTCATATTTCAA
TTTGGCTTTCACAATATTCCATATAAGGAATTAAGAAGGTGATATAGCAGTGGTTTTATAATTTTTGTTTTGTAGAGGGGAGCAACATGCAAAAATTCTCAAATTGCAATGTCTGAATGCTTAACTAATACTTGTAATATCCAATATCTAGTGAATGTATTTAGGGCTTCCCTAAACCATGTATTGAAGACTCTTTCAGG
ATCTGATTGTATGAAATAGTGTATATA

>TRINITY_DN125309_c0_g2_i11 from sample A1 / Example unspliced isoform:

TGAAAAGGCTACTTGCAATTTTGCAATTTTGACTGGTTCGAAAACGACACTCAGTCTGAAAATTAGGTTGGAGTCACATAAAAGTTCCGCAATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGATAGTGGCCTCAACCGGCTTCGTAACAATTCCAATTTCAATATTAGCCACCGACCAGgta
actatctttaatttattattggttactagtatatttacatggataggtgtttatatgttaaaatttggggtggattactatctatgtttttactagaatgaataggtgtttataagttagaatttaggtggattaccatctatgtttttactagattaaagaagattgaagaaggattagacaaatgtattattgattga
ttttatagtggtagttcaaaattccaaataaactattataaaaatatatatatataaaataatgtgtatggataactcctaactcatgcatggataaggtagtcacaaacagtagctgaggcatagataacttgtgattacaactaagcatggagaaccagaatcagataacttgttcttgttcatggcacttgtaacct
attgatgttcaccaaccacaaattaagtgatttatgctaattattatttttgttgtagatagtaacagtgatatctaattattggatgttgcaatcgcatgatgttgacgagatagGAGAAGGGAGTGAAGGAAATGCTGTGAATGTGGATGGAAAGCTATCTAAATTCAATATTCAACCCTCGTTATCCATGGAAAGCT
CTACGAGTCCGCCATCTTCACCAACTGCAGCCTCTTCTCCTTCATCTACTCCGCGAGATCAGGAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAATACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGTATCATTTACTCCTCTCCTACTCCATT
AAACAAGTTGCTTTTGTACAGTTTGACTGAGACACGCAGTTAAGAATAAGTGTGTTTTTTTTATTTTTAAAGTGAGTTCGATTGTAAAGAAGTAAGGTTTTTTACAGTTCTGTAAACTAACAAACCAAATCCATGTACCATATTGGGGTCTCCTAACCCAAAAAGTACAAAATTACAAGTCAATTTGAAGTCATTATTAC
CCTATTTTTACTTTGTTGTTGGTACCATTAAATCCTTCTGTCCCTGTTTTTGGGATGGCAACTTCTAAGCAGGCTTTACAGTGAAGACACGAAATCGAAAAGGGGCTGAGAACATTTTTCTGGAGGGATGTTACAAGTCCAAACAGTAATGTACAGTGTTCGAATGTGTTTCTGAGGATATAATTCCTTTTAGTGTGGAT
TGTAATTACCAAGTAGTTGTATTGTGTGATAAGAGGTGTAACCCATGAAGTCGGATGTTGAACTATCGTACACTGTCCAATTGAAGGGTATGAACATTTCAATTTGGTTCATATTTCAATTTGGCTTTCACAATATTCCATATAAGGAATTAAGAAGGTGATATAGCAGTGGTTTTATAATTTTTGTTTTGTAGAGGGGA
GCAACATGCAAAAATTCTCAAATTGCAATGTCTGAATGCTTAACTAATACTTGTAATATCCAATATCTAGTGAATGTATTTAGGGCTTCCCTAAACCATGTATTGAAGACTCTTTCAGGATCTGATTGTATGAAATAGTGTATATA

Intron 6 GTGAGTCAGT ... AATTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.77	301.3	87	51.3
A2	17.74	456.3	117.9	77.8
A3	13.68	338.1	66	39.6
A4	18.89	216.4	55	44.8
A5	22.57	255.9	88	60.6
A6	9.58	1237.3	134	100.8
A7	13.08	701.3	123	64
A8	10.04	597.5	74	40.5
A9	12.68	975.9	164	99.8
A10	11.33	509.3	51	63.3

>TRINITY_DN117256_c3_g2_i1 from sample A2 / Example spliced isoform

GAAAAGGCTACTTGCAATTTTGCAATTTTGACTGGTTCGAAAACGACACTCAGTCTGAAAATTAGGTTGGAGTCACATAAAAGTTCCGCAATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGATAGTGGCCTCAACCGGCTTCGTAACAATTCCAATTTCAATATTAGCCACCGACCAGGTAA
CTATCTTTAATTTATTATTGGTTACTAGTATATTTACATGGATAGGTGTTTATATGTTAAAATTTGGGGTGGATTACTATCTATGTTTTTACTAGAATGAATAGGTGTTTATAAGTTAGAATTTAGGTGGATTACCATCTATGTTTTTACTAGATTAAAGAAGATTGAAGAAGGATTAGACAAATGTATTATTGATTGAT
TTTATAGTGGTAGTTCAAAATTCCAAATAAACTATTATAAAAATATATATATATAAAATAATGTGTATGGATAACTCCTAACTCATGCATGGATAAGGTAGTCACAAACAGTAGCTGAGGCATAGATAACTTGTGATTACAACTAAGCATGGAGAACCAGAATCAGATAACTTGTTCTTGTTCATGGCACTTGTAACCTA
TTGATGTTCACCAACCACAAATTAAGTGATTTATGCTAATTATTATTTTTGTTGTAGATAGTAACAGTGATATCTAATTATTGGATGTTGCAATCGCATGATGTTGACGAGACAGGAGAAGGGAGTGAAGGAAATGCTGTGAATGTGGATGGAAAGCTATCTAAATTCAATATTCAACCCTCGTTATCCATGGAAAGCTC
TACGAGTCCGCCATCTTCACCAACTGCAGCCTCTTCTCCTTCATCTACTCCGCGAGATCAGGAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAACACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGCTTTACAGTGAAGACACGAAATCGAAAA
GGGGCTGAGAACATTTTTCTGGAGGGATGTTACAAGTCCAAACAGTAATGTACAGTGTTCGAATGTGTTTCTGAGGATATAATTCCTTTTAGTGTGGATTGTAATTACCAAGTAGTTGTATTGTGTGATAAGAGGTGTAACCCATGAAGTCGGATGTTGAACTATCGTACACTGTCCAATTGAAGGGTATGAACATTTCA
ATTTGGTTCATATTTCAATTTGGCTTTCACAATATTCCATATAAGGAATTAAGAAGGTGATATAGCAGTGGTTTTATAATTTTTGTTTTGTAGAGGGGAGCAACATGCAAAAATTCTCAAATTGCAATGTCTGAATGCTTAACTAATACTTGTAATATCCAATATCTAGTGAATGTATTTAGGGCTTCCCTAAACCATGT
ATTGAAGACTCTTTCAGGATCTGATTGTATGAAATAGTGTATATATAATTTTTTGGTTTAATTTATAATGGCTATAAAGATTTTGTAAGGGCATGTATTTATAGTACTAGATAGTATAAATGTGTTGTTTATAGTAATTATCGTATAGATATTTTTTTTCAATCTTATAAGTTATAAACCAAATTATAAACCATGATCAT
TAATATTTTAAGTATGTTGGGCC

>TRINITY_DN117256_c3_g2_i5 from sample A2 / Example unspliced isoform:

GAAAAGGCTACTTGCAATTTTGCAATTTTGACTGGTTCGAAAACGACACTCAGTCTGAAAATTAGGTTGGAGTCACATAAAAGTTCCGCAATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGATAGTGGCCTCAACCGGCTTCGTAACAATTCCAATTTCAATATTAGCCACCGACCAGGTAA
CTATCTTTAATTTATTATTGGTTACTAGTATATTTACATGGATAGGTGTTTATATGTTAAAATTTGGGGTGGATTACTATCTATGTTTTTACTAGAATGAATAGGTGTTTATAAGTTAGAATTTAGGTGGATTACCATCTATGTTTTTACTAGATTAAAGAAGATTGAAGAAGGATTAGACAAATGTATTATTGATTGAT
TTTATAGTGGTAGTTCAAAATTCCAAATAAACTATTATAAAAATATATATATATAAAATAATGTGTATGGATAACTCCTAACTCATGCATGGATAAGGTAGTCACAAACAGTAGCTGAGGCATAGATAACTTGTGATTACAACTAAGCATGGAGAACCAGAATCAGATAACTTGTTCTTGTTCATGGCACTTGTAACCTA
TTGATGTTCACCAACCACAAATTAAGTGATTTATGCTAATTATTATTTTTGTTGTAGATAGTAACAGTGATATCTAATTATTGGATGTTGCAATCGCATGATGTTGACGAGACAGGAGAAGGGAGTGAAGGAAATGCTGTGAATGTGGATGGAAAGCTATCTAAATTCAATATTCAACCCTCGTTATCCATGGAAAGCTC
TACGAGTCCGCCATCTTCACCAACTGCAGCCTCTTCTCCTTCATgtgagtcagtcccaagtgaacgtttttacctacttttctctgcctgagtagctttcacatgcatccatgcatatgcattatttttgtgaagtttggtacattaatggatagaagccagatgaaatcataaatggtgtatgaatgaattgtacagCT
ACTCCGCGAGATCAGGAGAACGTATGGAGAAGCGTGTTCAACCCAGGGAGTAACCCCAACACCAACTCAATTGGATCTCAAAGATTCGACAAAGTAGAACCGAACAGTCCTACTGTTTATGACTGGTATCATTTACTCCTCTCCTACTCCATTAAACAAGTTGCTTTTGTACAGTTTGACTGAGACACGCAGTTAAGAAT
AAGTGTGTTTTTTTTATTTTTAAAGTGAGTTCGATTGTAAAGAAGTAAGGTTTTTTACAGTTCTGTAAACTAACAAACCAAATCCATGTACCATATTGGGGTCTCCTAACCCAAAATGTACAAAATTACAAGTCAATTTGAAGTCATTATTACCCTATTTTTACTTTGTTGTTGGTACCATTAAATCCTTCTGTCCCTGT
TTTTGGGATGGCAACTTCTAAGCAGGCTTTACAGTGAAGACACGAAATCGAAAAGGGGCTGAGAACATTTTTCTGGAGGGATGTTACAAGTCCAAACAGTAATGTACAGTGTTCGAATGTGTTTCTGAGGATATAATTCCTTTTAGTGTGGATTGTAATTACCAAGTAGTTGTATTGTGTGATAAGAGGTGTAACCCATG
AAGTCGGATGTTGAACTATCGTACACTGTCCAATTGAAGGGTATGAACATTTCAATTTGGTTCATATTTCAATTTGGCTTTCACAATATTCCATATAAGGAATTAAGAAGGTGATATAGCAGTGGTTTTATAATTTTTGTTTTGTAGAGGGGAGCAACATGCAAAAATTCTCAAATTGCAATGTCTGAATGCTTAACTAA
TACTTGTAATATCCAATATCTAGTGAATGTATTTAGGGCTTCCCTAAACCATGTATTGAAGACTCTTTCAGGATCTGATTGTATGAAATAGTGTATATATAATTTTTTGGTTTAATTTATAATGGCTATAAAGATTTTGTAAGGGCATGTATTTATAGTACTAGATAGTATAAATGTGTTGTTTATAGTAATTATCGTAT
AGATATTTTTTTTCAATCTTATAAGTTATAAACCAAATTATAAACCATGATCATTAATATTTTAAGTATGTTGGGCC

Intron 7 GTAACGATCT ... GACGAGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.02	52.7	19	20.7
A2	24.48	65	26.7	17
A3	26.78	27.3	11.3	10.3
A4	43.35	15.7	18.3	6
A5	33.82	26	12.7	14.3
A6	15.48	216	40.3	42
A7	20.58	70.3	15	23.3
A8	15.88	40.3	7.3	9
A9	18.79	156.3	38.3	37
A10	18.38	88.7	22.7	18.7

>TRINITY_DN125309_c0_g2_i10 from sample A1 / Example spliced isoform

AAAAGGCTGCTTGCAATTTTGACAGGTTCGAAAACAAGACTCAGCCTGACTGAAAATTAGGTTGGAGTCACAGAAAAGTTGAGCGATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGACAGCGGCCTCAGAAGGCTTCGCAACAATTCCACTTTCAACGCTGGCCACGGAGCAAGCCGAGAAG
GGAGTCAGGGAAATGCTGTGAATGGAAAGCCCTCTAAATTCAATATTCAACGCTCGCTATCCATGGAAATCTCTACTAGTCCGCCATCTTCACCAACTGCAGTCACTTCCCCTTCATCTACTCCGCGAGATCGTGAGAACATATGGAGAAGCGTGTTCGACCCAGGCAGTAACATCAATACCAAGTCTATTGGATCTGAA
AAATTCGACAAAGCAGAACCAAACAGTCCTACTGTCTATGACTGGTTTTACAGTGGAGACACGAAATCGAAATGGCGCTGAGACCATTTTTACCTGGATGAATGGTACAACTCCAAACAATAATGTACAATGCTGGCGTGTGTTTCTGAGGATAGATTTGCTTTAGTGTGGGATTGTAATTACCAAGTATTTGCAGTATG
TGATAAGAAGTGTAAGCCATGAAGTCGGACGTTGAAATATCATACACTATCCTTTGTATTTTGGTGCTATGTACATACGAATATGAACATTACAATTTGGTTTCCACAATATTCCATAAGGGAGTTAAAAAACTGATATAGCAGTGGTTTTATGTTTTCTATCTTATAGAGGGTAGCAGTATGCAAAAATTCTACACTTC
CAAGTGATGAAATGTGC

>TRINITY_DN125309_c0_g2_i5 from sample A1 / Example unspliced isoform:

AAAAGGCTGCTTGCAATTTTGACAGGTTCGAAAACAAGACTCAGCCTGACTGAAAATTAGGTTGGAGTCACAGAAAAGTTGAGCGATCATGTTGGGTAAGATATGGGATGACACTCTTGGAGGTCCGCAGCCAGACAGCGGCCTCAGAAGGCTTCGCAACAATTCCACTTTCAACGCTGGCCACGGAGCAAgtaacgatc
tttgatccattgaaatttgaaactatttaaatcaatattattagttactagtattgttgtttttgttgttcgtcgcagtagcaagcaatacctaattattgggtgttgtaatcacatgatgatgatgttgacgagacagGCCGAGAAGGGAGTCAGGGAAATGCTGTGAATGGAAAGCCCTCTAAATTCAATATTCAACG
CTCGCTATCCATGGAAATCTCTACGAGTCCGCCATCTTCACCAACTGCAGTCACTTCCCCTTCATCTACTCCGCGAGATCGTGAGAACATATGGAGAAGCGTGTTCGACCCAGGCAGTAACATCAATACCAAGTCTATTGGATCTGAAAAATTCGACAAAGCAGAACCAAACAGTCCTACTGTCTATGACTGGTTTTACA
GTGGAGACACGAAATCGAAATGGCGCTGAGACCATTTTTACCTGGATGAATGGTACAACTCCAAACAATAATGTACAATGCTGGCGTGTGTTTCTGAGGATAGATTTGCTTTAGTGTGGGATTGTAATTACCAAGTATTTGCAGTATGTGATAAGAAGTGTAAGCCATGAAGTCGGACGTTGAAATATCATACACTATCC
TTTGTATTTTGGTGCTATGTACATACGAATATGAACATTACAATTTGGTTTCCACAATATTCCATAAGGGAGTTAAAAAACTGATATAGCAGTGGTTTTATGTTTTCTATCTTATAGAGGGTAGCAGTATGCAAAAATTCTACACTTCCAAGTGATGAAATGTGC