sp|F4ISQ7|TOP3B_ARATH ----> DNA topoisomerase 3-beta

Intron 1 GTAGGCATTT ... TTTGCGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.48	21.6	15	26
A2	68.56	22.9	9	45
A3	68.64	20.6	0	42
A4	55.86	24.1	15	28
A5	61.68	11.9	11	17
A6	65.04	203.6	64	336
A7	64.2	174.7	39	283
A8	70.23	80.6	19	173
A9	65.67	164.4	48	284
A10	65.99	145.6	42	251

>TRINITY_DN135416_c2_g1_i1 from sample A1 / Example spliced isoform

ACTACTTTTCGGAACTGGTTCAAGCGGATCAAGTAACCTTTTCCTTAGTATTGGGTCGTGGTAAATCTGAATTGGGTTTTTGTATTTTGCGACAGATCCAGGTTCCAATCACATAGGTGTGCGTTGTTGTGGATAATGGCGGTTATGACTATAAGAAATCCAGGTTCGAATCACACCAGAGGCCAGAGAAGAATGGCACT
GGAATGGGTACTTCTGGGTTACGCAGCAAGCGCAGAGGCCATTATGCTGTTGTTGCTCACAATGCCTCTAGGGATAAACCCCCTGAGAAAGGGATTGATTTCCGTCATTAGCAGTTCATTGAAGCCGATGCTCTGTTTGGTGTCATTCTGCCTGTTCTTACTCCTCGATATATTTTGGAAATACGAGACACTGGTCGCAT
GTGAAAAAGAGGATTGTAGCCAGAGCCTGCATATGAGCCACCAGAAATCAATCATGAAGAGCCAACGGAATGCTATCATGATTGCAGTGTCCCTGTTACTCTATTGGCTTCTCTTTTCTGTCACCACCATACTTCAGCGTCTTGATGTTTTGACTCAGCAGCTCAAGAACCTCCAGAGAGCCTCCCAATTGAGGAGGAGA
GTGAGCGAGCTTCAGTGAATCATCTCAATTGTTTTGGGCAAGGTTCAGTTGTAAGGGGAGCCTTATGCATTTGATGGACTTCTCTTGTTTTAGGAGGAGAGTGAGCTGAACCATCTCAATTGTTTTGGGGAAGGTTAACCAGGGAGTGTTGTCTTACTCACCTTGGGGGAAGGCAACAAGTCTATTTGGAATTTAAAGAT
TGCGAAGCCGTGAATCCTAGAGATGCTATATGCTGCTGTGTGCAGTGAAATTCTCTTCCTGCCAAGAATTATACTTTATTCCTCATATATCTGCAATTAGTGATCATACTTATAATAAAATGCTGTGTTGTATACTGTAAAGTTTCAAAGCATCACCACCTCCCAGTTGGGATTCTGTAGTTGATTTCATAGGTGTTATG
GGAGGGTGGTCACCAACATAATTTTTTTCCTCTAAAGGATGCACATAATCACCTCAATTAGGTTTTTGCTTTGTATCTCAAGAGTATTTTCAAAATCAAATGTTAAAGTATCTTCAAAATCAAATGTGG

>TRINITY_DN135416_c2_g1_i3 from sample A1 / Example unspliced isoform:

ACTACTTTTCGGAACTGGTTCAAGCGGATCAAGTAACCTTTTCCTTAGTATTGGGTCGTGGTAAATCTGAATTGGGTTTTTGTATTTTGCGACAGATCCAGGTTCCAATCACATAGGTGTGCGTTGTTGTGGATAATGGCGGTTATGACTATAAGAAgtaggcatttacacgctaactctttggattaatatatatatat
atatatatactctgtttgttagctatggaagaatctacatttgcatatatacactacttttcaaaactggttcaagcggatcaaataagctttttccttgtactgggtcgtcgcaaatctgaattgggtctttgtattttgcggtagATCCAGGTTCGAATCACACCAGAGGCCAGAGAAGAATGGCACTGGAATGGGTA
CTTCTGGGTTACGCAGCAAGCGCAGAGGCCATTATGCTGTTGTTGCTCACAATGCCTCTAGGGATAAACCCCCTGAGAAAGGGATTGATTTCCGTCATTAGCAGTTCATTGAAGCCGATGCTCTGTTTGGTGTCATTCTGCCTGTTCTTACTCCTCGATATATTTTGGAAATACGAGACACTGGTCGCATGTGAAAAAGA
GGATTGTAGCCAGAGCCTGCATATGAGCCACCAGAAATCAATCATGAAGAGCCAACGGAATGCTATCATGATTGCAGTGTCCCTGTTACTCTATTGGCTTCTCTTTTCTGTCACCACCATACTTCAGCGTCTTGATGTTTTGACTCAGCAGCTCAAGAACCTCCAGAGAGCCTCCCAATTGAGGAGGAGAGTGAGCGAGC
TTCAGTGAATCATCTCAATTGTTTTGGGCAAGGTTCAGTTGTAAGGGGAGCCTTATGCATTTGATGGACTTCTCTTGTTTTAGGAGGAGAGTGAGCTGAACCATCTCAATTGTTTTGGGGAAGGTTAACCAGGGAGTGTTGTCTTACTCACCTTGGGGGAAGGCAACAAGTCTATTTGGAATTTAAAGATTGCGAAGCCG
TGAATCCTAGAGATGCTATATGCTGCTGTGTGCAGTGAAATTCTCTTCCTGCCAAGAATTATACTTTATTCCTCATATATCTGCAATTAGTGATCATACTTATAATAAAATGCTGTGTTGTATACTGTAAAGTTTCAAAGCATCACCACCTCCCAGTTGGGATTCTGTAGTTGATTTCATAGGTGTTATGGGAGGGTGGT
CACCAACATAATTTTTTTCCTCTAAAGGATGCACATAATCACCTCAATTAGGTTTTTGCTTTGTATCTCAAGAGTATTTTCAAAATCAAATGTTAAAGTATCTTCAAAATCAAATGTGG