sp|Q8S9L5|TIG_ARATH ----> Trigger factor-like protein TIG, Chloroplastic

Intron 1 GTATTCAAGC ... AAACTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.3	46	53	52
A2	49.02	52	48	52
A3	53.93	41	49	47
A4	55.37	27	33	34
A5	42.86	2	2	1
A6	49.86	91	92	89
A7	49.57	203	197	202
A8	52.74	324	364	359
A9	50.79	219	221	231
A10	53.89	148	168	178

>TRINITY_DN44576_c1_g2_i3 from sample A8 / Example spliced isoform

ACATACTTAACTTCAAATTTTCGGGGGGTCTATAAAAACATCTTATCCAATTTCATTTGAACGAAAGAATCACCACAGGAGGTTTCGGGCTTGTTCTGAACAGCCTTTGCAGTCATCCATTCTCCACTTGTTTTTCTTTTCCTTACCAGGATTAGAAGTAGTATCCTTATTTCTACAAGGCAAAATGTCAGTGACAATGA
AGATGGAGCTGTTGAACCCATATGGTAGTGCCTGTTTATGGCCTATCTCATCTTCTTCTTCTGCTAAATTAGCTGTCTCATTGGGTGCTTGCAGAACACACTTAGCTTGCTTCACCAGGACATCACCGTCTGAGAATACATTAACATTACCACGCTCAAACACTCAACACCACAAATGTGTGTATATATGGAAGAAGCAA
AGAAGAAGGGATGCATTCTGCCTGGCTTTCGCTGCAGCTGCAGTTGCTGCTGTCAAGGATGACGAGGGAAACTCAAAGACAGATGTTCAAGTTACAGAAATAAAAGAGCCAAACTGCAGAGTCAGGCTCAATGTGAAAGTCCCTCCAAGAATATGTAAAGAAAGTTATGAGGAAGTTTTGGAAGAACTGCGCAAACAAAC
ACAGATTCCTGGATTTCGCCGTGGTGAGAAAATTCCAGATAAAATTCTTATAAACTTCATCGGGAAAGAAAAAATAAAGACTGCAGCAATTGAAGCAGTTCTGAATAAGACTCTTCCAGAAGCAATGTCTTCAGTTAAGGACAGAGCATTGAAGGATTCAGAGCATATAGAGACCAAGTATAATGCATTGCAAGCTGCTT
TCTCTCCGTCAAGTTTTCTCAGCTATGATGTTGCAGTCGATTTGGCACCTGAAGTTAAATGGACTTCACCTGAAGCATACAAGAACCTAAAGGTTGTAGTTGAAGTTGAAGGCGATTCTGCCATAGAAAGAGCTGCTGAAGCTGAACTGAAGGCTCGTTATAAGAATTTGGGATCTCTAAAAATTGTTCAAGGCAGAGGT
CTTAAGATTGGAGATGTTGTGGTTCTCGATGTCTCGGCTGCACAAATAAATGAAGATGGAACAGATGGTGATAAACTCCCATTCACTGAGCAGAAAGGTTTTCATTTGGACACTGAAGAGAGCACTCAGCTTGTTCCAGGATTTGTTAATGCAATATTAGGACTTGAAACAGGACAAACACGGTCTTTTCAGCTTGCATT
TCCAGATACATGGAAGCAAAAGGAGCTTCAAGGAGTTCTTGCTCGTTTTACTGTTGAGTGCAAGGAATTATTTTACAGAGATCTACCGGTTTTGGATGACACGCTTGCCGATAAGCTTGTAAATGGTTTTAGCTCTTTGAGTCAGGTTCAAGAATCAATCTTACAAGAGCACAAGAAAATGGCAGAAGCGAAAAAGAAAC
AAATAATCCAACGAGCACTTCTGCAAGAACTTTCCAAGGTTGTAGAAATGGATGTTCCCCATTCCTTACTAGAAGAGCATGGTCGGCAAGTGTATGGAGCCAAGCTGATACAGCTGCAGATAACAATGAAACTGACTGAGGATCAGATTTATACCCTCTCAAGTGCAAAGGCGGTCAACAACTACCTCTTATCACAAAAG
GAGTCAATCAAGGAAATAGTCAAACAAACATTTGCAGTTGAAGAATACTCAGAGGAAGAGCTGGAAAAAGAGACGGAAGATACTGCAGCAGAATTCAAAGGCTATAATCATGAATTTGATAGAGACCGTATTAGAGAGCAGGCACAGGAATTCCTTAAGGGAGAAAAGGTCTTGCAATTTTTAATGCAACATGCAGATAT
CACTTACATATTAAACGATGCATAAGAGCTTGGACTAGTAAAGAGGACTTAGCTTCAATAATTTTTGCCCTGTCTGAAACAACATGAAATGACATTTTTAAATTTAAGTACTGTATGAGGTAAGTGATTTGGGCTGTACAAATATTTAACCCTTTATAGGACAATGATTGTCTATCAACATAGAGTACAATGGATTATCT
GTTTTTCTGGGTATCACCCATTCCCAAAAATTGAGCACCAAAATTGTGTTCTTTGAGGTTCTATTTCAATTGATTTGAATAATTTGCAATGTCTCTTTAAGGCCTATCATACGAATCCATGTTTTGAAAATTCTCAAATTTTATTCAACTGTATCTCCATTTCGTG

>TRINITY_DN44576_c1_g2_i1 from sample A8 / Example unspliced isoform:

ACATACTTAACTTCAAATTTTCGGGGGGTCTATAAAAACATCTTATCCAATTTCATTTGAACGAAAGAATCACCACAGGAGGTTTCGGGCTTGTTCTGAACAGCCTTTGCAGTCATCCATTCTCCACTTGTTTTTCTTTTCCTTACCAGGATTAGAAGTAGTATCCTTATTTCTACAAGGCAAAATGTCAGTGACAATGA
AGATGGAGCTGTTGAACCCATATGGTAGTGCCTGTTTATGGCCTATCTCATCTTCTTCTTCTGCTAAATTAGCTGTCTCATTGGGTGCTTGCAGAACACACTTAGCTTGCTTCACCAGGACATCACCGTCTGAGAATACATTAACATTACCACGCTCAAACACTCAACACCACAAATGTGTGTATATATGGAAGAAGCAA
AGAAGAAGGGATGCATTCTGCCTGGCTTTCGCTGCAGCTGCAGTTGCTGCTGTCAAGGATGACGAGGGAAACTCAAAGACAGATGTTCAAGTTACAGAAATAAAAGAGCCAAACTGCAGAGTCAGGCTCAATGTGAAAGTCCCTCCAAGAATATGTAAAGAAAGTTATGAGGAAGTTTTGGAAGAACTGCGCAAACAAAC
ACAGATTCCTGGATTTCGCCGTGGTGAGAAAATTCCAGATAAAATTCTTATAAACTTCATCGGGAAAGAAAAAATAAAGACTGCAGCAATTGAAGCAGTTCTGAATAAGACTCTTCCAGAAGCAATGTCTTCAGTTAAGGACAGAGCATTGAAGGATTCAGAGCATATAGAGACCAAGTATAATGCATTGCAAGCTGCTT
TCTCTCCGTCAAGTTTTCTCAGCTATGATGTTGCAGTCGATTTGGCACCTGAAGTTAAATGGACTTCACCTGAAGCATACAAGAACCTAAAGGTTGTAGTTGAAGTTGAAGGCGATTCTGCCATAGAAAGAGCTGCTGAAGCTGAACTGAAGGCTCGTTATAAGAATTTGGGATCTCTAAAAATTGTTCAAGGCAGAGGT
CTTAAGATTGGAGATGTTGTGGTTCTCGATGTCTCGGCTGCACAAATAAATGAAGATGGAACAGATGGTGATAAACTCCCATTCACTGAGCAGAAAGGTTTTCATTTGGACACTGAAGAGAGCACTCAGCTTGTTCCAGGATTTGTTAATGCAATATTAGGACTTGAAACAGGACAAACACGGTCTTTTCAGCTTGCATT
TCCAGATACATGGAAGCAAAAGGAGCTTCAAGGAGTTCTTGCTCGTTTTACTGTTGAGTGCAAGGAATTATTTTACAGAGATCTACCGGTTTTGGATGACACGCTTGCCGATAAGCTTGTAAATGGTTTTAGCTCTTTGAGTCAGGTTCAAGAATCAATCTTACAAGAGCACAAGAAAATGGCAGAAGCGAAAAAGAAAC
AAATAATCCAACGAGCACTTCTGCAAGAACTTTCCAAGGTTGTAGAAATGGATGTTCCCCATTCCTTACTAGAAGAGCATGGTCGGCAAGTGTATGGAGCCAAGCTGATACAGCTGCAGATAACAATGAAACTGACTGAGGATCAGATTTATACCCTCTCAAGTGCAAAGGCGGTCAACAACTACCTCTTATCACAAAAG
GAGTCAATCAAGGAAATAGTCAAACAAACATTTGCAGTTGAAGAAgtattcaagcttgaaaacttgcagTACTCAGAGGAAGAGCTGGAAAAAGAGACGGAAGATACTGCAGCAGAATTCAAAGGCTATAATCATGAATTTGATAGAGACCGTATTAGAGAGCAGGCACAGGAATTCCTTAAGGGAGAAAAGGTCTTGCA
ATTTTTAATGCAACATGCAGATATCACTTACATATTAAACGATGCATAAGAGCTTGGACTAGTAAAGAGGACTTAGCTTCAATAATTTTTGCCCTGTCTGAAACAACATGAAATGACATTTTTAAATTTAAGTACTGTATGAGGTAAGTGATTTGGGCTGTACAAATATTTAACCCTTTATAGGACAATGATTGTCTATC
AACATAGAGTACAATGGATTATCTGTTTTCTGAAGTTAATCATTCTGGGTATCACCCATTCCCAAAAATTGAGCACCAAAATTGTGTTCTTTGAGGTTCTATTTCAATTGATTTGAATAATTTGCAATGTCTCTTTAAGGCCTATCATACGAATCCATGTTTTGAAAATTCTCAAATTTTATTCAACTGTATCTCCATTT
CGTG

Intron 2 GTCAGGCTCA ... ACAAACACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	68.54	28	64	58
A2	77.3	16	56	53
A3	72.09	24	69	55
A4	74.19	20	58	57
A5	78.18	6	21	22
A6	90.24	2	20	17
A7	71.65	36	92	90
A8	70.33	31	72	75
A9	73.18	35	95	96
A10	69.86	33	73	80

>TRINITY_DN46585_c0_g5_i2 from sample A10 / Example spliced isoform

GCGGATACTCAAATTGAACAGGAGACACGAGGACACATGCCCCTGGGTTTGGAGGAGGACATACTTAACTTCAAATTTTCGGGGGGTCTATAAAAACATCTTATCCAATTTCATTTGAACGAAAGAATCACCACAGGAGGTTTCGGGCTTGTTCTGAACAGCCTTTGCAATCATACATCCTCCACTTGTTTTTCTTTTCC
TTACCAGGATTAGAAGTAGTATCCTTATTTCTACAAGGCAAAATGTCAGTGACAATGAAGATGGAGCTGCTGAACCCATATGGCAGTGCCTGTTTATGGCCTATCTCATCTTCTTCTTCTGCTAAATTAGCTGTCTCATTGGGTGCTTGCAGAACACACTTAGCTTGCTTCAGCAGGACATCACGGTCTGAGAATACATT
AACATTACCACGCTCAAACACTCAACACCACAAATGTGTGTATATATGGAAGAAGCAAAGAAGAAGGGATGCATTCTGCCTGGCTTTCGCTGCAGCTGCAGTTGCTGCTGTCAAGGATGACGAGGGAAACTCAAAGACAGATGTTCAAGTTACAGAAATAAAAGAGCCAAACTGCAGAATTCCTGGATTTCGCCGTGGTG
AGAAAATTCCAGATAAAATTCTTATAAACTTCATCGGGAAAGAAAAAATAAAGACTGCAGCAATTGAAGCAGTTCTGAATAAGACTCTTCCAGAAGCAATGTCTTCAGTTAAGGACAGAGCATTGAAGGATTCAGAGCATATAGAGACCAAGTATAATGCATTGCAAGCTGCTTTCTCTCCGTCAAGTTTTCTCAGCTAT
GATGTTGCAGTCGATTTGGCACCTGAAGTTAAATGGACTTCACCTGAAGCATACAAGAACCTAAAGGTTGTAGTTGAAGTTGAAGGCGATTCTGCCATAGAAAGAGCTGCTGAAGCTGAACTGAAGGCTCGTTATAAGAATTTGGGATCTCTAAAAATTGTTCAAGGCAGAGGTCTTAAGATTGGAGATGTTGTGGTTCT
CGATGTCTCGGCTGCACAAATAAATGAAGATGGAACAGATGGTGATAAACTCCCATTCACTGAGCAGAAAGGTTTTCATTTGGACACTGAAGAGAGCACTCAACTTGTTCCAGGATTTGTTAATGCAATATTAGGACTTGAAACAGGACAAACACGGTCTTTTCAGCTTGCATTTCCAGATACATGGAAGCAAAAGGAGC
TTCAAGGAGTTCTTGCTCGTTTTACTGTTGAGTGCAAGGAATTATTTTACAGAGATCTACCGGTTTTGGATGACACGCTTGCCGATAAGCTTGTAAATGGTTGTAGCTCTTTGAGTCAGGTTCAAGAATCAATCTTACAAGAGCACAAGAAAATGGCAGAAGCGAAAAAGAAACAAATAATCCAACGAGCACTTCTGCAA
GAACTTTCCAAGGTTGTAGAAATGGATGTTCCCCATTCCTTACTAGAAGAGCATGGTCGGCAAGTGTATGGAGCCAAGCTGATACAGCTGCAGATAACAATGAAACTGACTGAGGATCAGATTTATACCCTCTCAAGTGCAAAGGCGGTCAACAACTACCTCTTATCACAAAAGGAGTCAATCAAGGAAATAGTCAAACA
AACATTTGCAGTTGAAGAAGTATTCAAGCTTGAAAACTTGCAGTACTCAGAGGAAGAGCTGGAAAAAGAGACGGAAGATACTGCAGCAGAATTCAAAGGCTATAATCATGAATTTGATAGAGACCGTATTAGAGAGCAGGCACAGGAATTCCTTAAGGGAGAAAAGGTCTTGCAATTTTTAATGCAACATGCAGATATCA
CATACATATTAAACGATGCATAAGAGCTTGGACTAGTAAAGAGGACTTAGCTTCAATAATTTTTGCCCTGTCTGAAACAACATGAAATGACATTTTTAAATTTAAGTACTGTATGAGGTAAGTGATTTGGGCTGTATAAATATTTAACCCTTTATAGGACAATGATTGTTTATCAACATAGAGTACAATGGATTATCTGT
TTT

>TRINITY_DN46585_c0_g5_i3 from sample A10 / Example unspliced isoform:

GCGGATACTCAAATTGAACAGGAGACACGAGGACACATGCCCCTGGGTTTGGAGGAGGACATACTTAACTTCAAATTTTCGGGGGGTCTATAAAAACATCTTATCCAATTTCATTTGAACGAAAGAATCACCACAGGAGGTTTCGGGCTTGTTCTGAACAGCCTTTGCAGTCATCCATTCTCCACTTGTTTTTCTTTTCC
TTACCAGGATTAGAAGTAGTATCCTTATTTCTACAAGGCAAAATGTCAGTGACAATGAAGATGGAGCTGTTGAACCCATATGGTAGTGCCTGTTTATGGCCTATCTCATCTTCTTCTTCTGCTAAATTAGCTGTCTCATTGGGTGCTTGCAGAACACACTTAGCTTGCTTCACCAGGACATCACCGTCTGAGAATACATT
AACATTACCACGCTCAAACACTCAACACCACAAATGTGTGTATATATGGAAGAAGCAAAGAAGAAGGGATGCATTCTGCCTGGCTTTCGCTGCAGCTGCAGTTGCTGCTGTCAAGGATGACGAGGGAAACTCAAAGACAGATGTTCAAGTTACAGAAATAAAAGAGCCAAACTGCAGAgtcaggctcaatgtgaaagtcc
ctccaagaatatgtaaagaaagttatgaggaagttttggaagaactgcgcaaacaaacacagATTCCTGGATTTCGCCGTGGTGAGAAAATTCCAGATAAAATTCTTATAAACTTCATCGGGAAAGAAAAAATAAAGACTGCAGCAATTGAAGCAGTTCTGAATAAGACTCTTCCAGAAGCAATGTCTTCAGTTAAGGAC
AGAGCATTGAAGGATTCAGAGCATATAGAGACCAAGTATAATGCATTGCAAGCTGCTTTCTCTCCGTCAAGTTTTCTCAGCTATGATGTTGCAGTCGATTTGGCACCTGAAGTTAAATGGACTTCACCTGAAGCATACAAGAACCTAAAGGTTGTAGTTGAAGTTGAAGGCGATTCTGCCATAGAAAGAGCTGCTGAAGC
TGAACTGAAGGCTCGTTATAAGAATTTGGGATCTCTAAAAATTGTTCAAGGCAGAGGTCTTAAGATTGGAGATGTTGTGGTTCTCGATGTCTCGGCTGCACAAATAAATGAAGATGGAACAGATGGTGATAAACTCCCATTCACTGAGCAGAAAGGTTTTCATTTGGACACTGAAGAGAGCACTCAACTTGTTCCAGGAT
TTGTTAATGCAATATTAGGACTTGAAACAGGACAAACACGGTCTTTTCAGCTTGCATTTCCAGATACATGGAAGCAAAAGGAGCTTCAAGGAGTTCTTGCTCGTTTTACTGTTGAGTGCAAGGAATTATTTTACAGAGATCTACCGGTTTTGGATGACACGCTTGCCGATAAGCTTGTAAATGGTTGTAGCTCTTTGAGT
CAGGTTCAAGAATCAATCTTACAAGAGCACAAGAAAATGGCAGAAGCGAAAAAGAAACAAATAATCCAACGAGCACTTCTGCAAGAACTTTCCAAGGTTGTAGAAATGGATGTTCCCCATTCCTTACTAGAAGAGCATGGTCGGCAAGTGTATGGAGCCAAGCTGATACAGCTGCAGATAACAATGAAACTGACTGAGGA
TCAGATTTATACCCTCTCAAGTGCAAAGGCGGTCAACAACTACCTCTTATCACAAAAGGAGTCAATCAAGGAAATAGTCAAACAAACATTTGCAGTTGAAGAAGTATTCAAGCTTGAAAACTTGCAGTACTCAGAGGAAGAGCTGGAAAAAGAGACGGAAGATACTGCAGCAGAATTCAAAGGCTATAATCATGAATTTG
ATAGAGACCGTATTAGAGAGCAGGCACAGGAATTCCTTAAGGGAGAAAAGGTCTTGCAATTTTTAATGCAACATGCAGATATCACATACATATTAAACGATGCATAAGAGCTTGGACTAGTAAAGAGGACTTAGCTTCAATAATTTTTGCCCTGTCTGAAACAACATGAAATGACATTTTTAAATTTAAGTACTGTATGA
GGTAAGTGATTTGGGCTGTATAAATATTTAACCCTTTATAGGACAATGATTGTTTATCAACATAGAGTACAATGGATTATCTGTTTTCTGAAGTTAATCATTCTGGGTATCACCCATTCCCAAAAATTGAGCACCAAAATTGTGTTCTTTGAGGTTCTATTTCAATTGATTTGAATAATTTGCAATGTCTCTTTAAGGCC
TATCATACGAATCCA