sp|Q6H7S2|RH8_ORYSJ ----> DEAD-box ATP-dependent RNA helicase 8

Intron 1 GTAAGTGGCT ... TTTCCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.79	11	28	0
A2	53.85	24	28	0
A3	60.66	24	37	0
A4	68.42	12	26	0
A5	71.43	8	20	0
A6	72	35	90	0
A7	61.99	65	106	0
A8	59.39	67	98	3
A9	65.03	64	119	1
A10	70.68	39	94	0

>TRINITY_DN43620_c0_g2_i5 from sample A9 / Example spliced isoform

GGGGAAGGAAAAGTGAATTACTAAAAAAATGAGTAAATTAAAAAAAAGAATTGGAGGGAAAATAAATGAAGCTTAAATAGATCCAATCTGTCCGAGTTGAATGGATGCCAAAAAAGGGTTGAATCATGCCAAAACTGACAACCTGATCAAGAAATTCAGCATCTAGCTCTGTCCTGACAGTAAAGATCGTTGGGTGGGCT
AACAATGCGGGTAGTGCACACAGCTTACCCTGTAGGTCCATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGATCCAAATCAACCAATTAATATATTTTTTCAATTATGTTAATTGAGTGGAACTGTTGGCCAA
AGAAATCACAGAACTTGGATATTCATGCTTTTACATCCATGCAAAGATGTTACGATCTCACCAGAACAAAGTCTTCCATGACTTCCATAATGGTGCCTGTAGAAATCTAGTGTCTTCAGATGAAAGGCTTGCTAAAAGTGAAGAATGCTTTCGCACAATCGTTCCAGTGTATCCGCACCAAATGTGAACAACTAATGATA
CATAGCTCATCAAATTGCATAGTTTGCAAAGAAGCAACATAAGTGAGATATGAGCACAATGTTGAATGCATTATAGAAACAGAGGATGGGCCAATTAAATGTACCTTACGTAGTCGAGCACTTGAAGAATGTTTCCCAGAAATTGGGAAAATATCTTATGAGAATTACTCAAAAAAGATTTCGTCAAGTGTGTTGTGTTT
GCAAAAACTATGCTAGACAGGCCTTTTCACACTGTCAGTAGGCAACAACATCACATAACTAAAAAAACTATGATCACTGTCTCTGAAGTCGTTGCAATGTTTTTTTGTTGTCAAGGAACAATTACTTTTCCAAAGAATAAATTTGGATTAGTAGTATGGGTTGTTAGCAATTACTTTTCAGACTTCTTCTAACCTATTCT
CCATAGTTTTATCTCTATTGAATCCATAACTTATATTAATGTTACTAAAGTTGCATAAGGGAATTTTGGTCTAAACCTGGAGCCTTCACAACTCTTTTTGAAGAGAAGGAAAATAGGGGGGGAATGCTGACAATACATCGATATTGTTGTTCAATCATACCTGCAGTTTTGTATTTCAATATCTGTTACATTCCAAATGC
AATTTAGAAGTTTTTTTCTG

>TRINITY_DN43620_c0_g2_i8 from sample A9 / Example unspliced isoform:

GGGGAAGGAAAAGTGAATTACTAAAAAAATGAGTAAATTAAAAAAAAGAATTGGAGGGAAAATAAATGAAGCTTAAATAGATCCAATCTGTCCGAGTTGAATGGATGCCAAAAAAGGGTTGAATCATGCCAAAACTGACAACCTGATCAAGAAATTCAGCATCTAGCTCTGTCCTGACAGTAAAGATCGTTGGGTGGGCT
AACAATGCGGGTAGTGCACACAGCTTACCCTGTAGGTCCATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGgtaagtggctttccccttttgggtgcttccaacttagggtttggcgttcttgttggaagaac
ttccgagtatggaaacattggtatctttaaagggaagaacttgcctagtgcgtgctcctagtcccaaacttctacaacgggctcaagctttgggcaagctattacatcaaaatagccttctggagaattctatctcctgctttcttgatgaaaaccaaaactagtggattgatgatcactcgtccgatgatagtcacgct
aatggcactttccctacggatcaacgtctggaattttgatattcacgctaatggcactctcataaatttcaatcttcgcaaaatttcttgcctcaactctaggttggctttatcattttgcttcataaaagtgcgctcatttgttttgtttggatgtgagctaaatcttaagcattattaaaagttttgttgttttcctg
cagATCCAAATCAACCAATTAATA

Intron 2 GTAAGTGGCT ... TCAACTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.16	11	28	17
A2	50.52	24	28	21
A3	51.02	24	37	13
A4	56.36	12	26	5
A5	62.79	8	20	7
A6	66.18	35	90	47
A7	59.12	65	106	82
A8	57.32	67	98	82
A9	60.62	64	119	78
A10	62.32	39	94	35

>TRINITY_DN39070_c0_g1_i7 from sample A7 / Example spliced isoform

GTAAATTAAAAAAAAGAATTGGAGGGAAAATAAATGAAGCTTAAATAGATCAAATCTGTCCGAGTTGAATGGATGCCAAAAAAGGGTTGAATCATGCCAAAACTGACAACCTGATCAAGAAATTCAGCATCTAGCTCTGTCCTGACAGTAAAGATCGTTGGGTGGGCTAACAATGCGGGTAGTGCACACAGCTTACCCTG
TAGGTCCATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGATCCAAATCAACCAATTAATATATTTTTTCAATTATGTTAATTGAGTGGAACTGTTGGCCAAAGAAATCACAGAACTTGGATATTCATGCTTTT
ACATCCATGCAAAGATGTTACGATCTCACCAGAACAAAGTCTTCCATGACTTCCATAATGGTGCCTGTAGAAATCTAGTGTCTTCAGATGAAAGGCTTGCTAAAAGTGAAGAATGCTTTCGCACAATCGTTCCAGTGTATCCGCACCAAATGTGAACAACTAATGATACATAGCTCATCAAATTGCATAGTTTGCAAAGA
AGCAACATAAGTGAGATATGAGCACAATGTTGAATGCATTCTAGAAACAGAGGATGGGCCAATTAAATGTACCTTACGTAGTCGAGCACTTGAAGAATGTTTCCCAGAAATTGGGAAAATATCTTATGAGAATTACTCAAAAAAGATTTCGTCAAGTGTGTTGTGTTTGCAAAAACTATGCTAGACAGGCCTTTTCACAC
TGTCAGTAGGCAACAACATCACATAACTAAAAAAACTATGATCACTGTCTCTGAAGTCGTTGCAATGTTTTTTGTTGTCAAGGAACAATTACTTTTCCAAAGAATAAATTTGGATTAGTAGTATGGGTTGTTAGCAATTACTTTTCAGACTTCTTCTAACCTATTCTCCATAGTTTTATCTCTATTGAATCCATAACTTA
TATTAATGTTACTAAAGTTGCATAAGGGAATTTTGGTCTAAACCTGGAGCCTTCACAACTCTTTTTGAAGAGAAGGAAAATAGGGGGGGAATGCTGACAATACATCGATATTGTTGTTCAATCATACCTGCAGTTTTGTATTTCAATATCTG

>TRINITY_DN39070_c0_g1_i4 from sample A7 / Example unspliced isoform:

GTAAATTAAAAAAAAGAATTGGAGGGAAAATAAATGAAGCTTAAATAGATCAAATCTGTCCGAGTTGAATGGATGCCAAAAAAGGGTTGAATCATGCCAAAACTGACAACCTGATCAAGAAATTCAGCATCTAGCTCTGTCCTGACAGTAAAGATCGTTGGGTGGGCTAACAATGCGGGTAGTGCACACAGCTTACCCTG
TAGGTCCATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGgtaagtggctttccccttttgggtgcttccaacttagggtttggcgttcttgttggaagaacttccgagtatggaaacattggtatctttaaag
ggaagaacttgcctagtgcgtgctcctagtcccaaacttctacaacgggctcaagctttgggcaagctattacatcaaaatagccttctggagaattctatctcctgctttcttgatgaaaaccaaaactagtggattgatgatcactcgtccgatgatagtcacgctaatggcactttccctacggatcaacgtctgga
attttgatattcacgctaatggcactcataaatttcaatcttcgcaaaatttcttgcctcaactctagATCCAAATCAACCAATTAATATATTTTTTCAATTATGTTAATTGAGTGGAACTGTTGGCCAAAGAAATCACAGAACTTGGATATTCATGCTTTTACATCCATGCAAAGATGTTACGATCTCACCAGAACAAA
GTCTTCCATGACTTCCATAATGGTGCCTGTAGAAATCTAGTGTCTTCAGAAATACTAACAGGATGGAGAACAAAATGGACAAAACATCAACTAGTGATACACACAAAAAGGTGCATTTTTTATTATTATTCATCCTATACAAATACAAGCAAATTCCCAAAGTTGCTATATAGTAGATTATGGTCCTAATACATATTTAT
TTTTCTCGTTCTTTCTTTTGCCTATTTACAATAATGCAACTATGCTCGATGATATATAACAAAGTTTTTGAGGGAGACGTGTTAGCACTATATGGACATGGCCAGAGCAAAGGGCCAAATTCATTGACAAAAACACAATTAGTACATGCTGATCTAGTAGACAAGGATGGACAGATGACAATAACTGTATACATCAACAA
CCACTTAATCCCTTGTTTCATTGATTAATTCTGTCCTGGAGTTAGTGTCAGAATAGTCGACTTTGTCTTGAAGAAAAAATGACCATACCATAAAGGAGATGCGAAATTCTGCATTGTCCTCCTTGATAGAACAAAATTTGAGTTTATTGAGCAGGTTTGCAAATATCACAAATTTCCACCGGACACCATAATTGATGAGT
TAGTGCAATCAAATGATGCCTATTCAGTTGGTTCTATTGCAGCAATTGTCATTGGATGTCAATAAAACAACACACAATACGATCTAAGTATCAAGGATGGAGAGATGCCATAGACAATGCAATTGTAAGTCTTGACCATTTTTATCAAAAATACTTTTTCTTTGTAGATGAAAGGCTTGCTAAAAGTGAAGAATGCTTTC
GCACAATCGTTCCAGTGTATCCGCACCAAATGTGAACAACTAATGATACATAGCTCATCAAATTGCATAGTTTGCAAAGAAGCAACATAAGTGAGATATGAGCACAATGTTGAATGCATTCTAGAAACAGAGGATGGGCCAATTAAATGTACCTTACGTAGTCGAGCACTTGAAGAATGTTTCCCAGAAATTGGGAAAAT
ATCTTATGAGAATTACTCAAAAAAGATTTCGTCAAGTGTGTTGTGTTTGCAAAAACTATGCTAGACAGGCCTTTTCACACTGTCAGTAGGCAACAACATCACATAACTAAAAAAACTATGATCACTGTCTCTGAAGTCGTTGCAATGTTTTTTGTTGTCAAGGAACAATTACTTTTCCAAAGAATAAATTTGGATTAGTA
GTATGGGTTGTTAGCAATTACTTTTCAGACTTCTTCTAACCTATTCTCCATAGTTTTATCTCTATTGAATCCATAACTTATATTAATGTTACTAAAGTTGCATAAGGGAATTTTGGTCTAAACCTGGAGCCTTCACAACTCTTTTTGAAGAGAAGGAAAATAGGGGGGGAATGCTGACAATACATCGATATTGTTGTTCA
ATCATACCTGCAGTTTTGTATTTCAATATCTG

Intron 3 GTGCATTTTT ... TTCTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40	9	0	12
A2	44.44	1	1.7	0
A3	77.78	1	5	2
A4	33.33	4	1	3
A5	-1	0	0	0
A6	20.64	5	2.7	0
A7	50.39	10	9.3	11
A8	36.03	13	2.7	12
A9	43.75	9	3	11
A10	33.33	15	1	14

>TRINITY_DN39070_c0_g1_i5 from sample A7 / Example spliced isoform

GTAAATTAAAAAAAAGAATTGGAGGGAAAATAAATGAAGCTTAAATAGATCAAATCTGTCCGAGTTGAATGGATGCCAAAAAAGGGTTGAATCATGCCAAAACTGACAACCTGATCAAGAAATTCAGCATCTAGCTCTGTCCTGACAGTAAAGATCGTTGGGTGGGCTAACAATGCGGGTAGTGCACACAGCTTACCCTG
TAGGTCCATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGGTAAGTGGCTTTCCCCTTTTGGGTGCTTCCAACTTAGGGTTTGGCGTTCTTGTTGGAAGAACTTCCGAGTATGGAAACATTGGTATCTTTAAAG
GGAAGAACTTGCCTAGTGCGTGCTCCTAGTCCCAAACTTCTACAACGGGCTCAAGCTTTGGGCAAGCTATTACATCAAAATAGCCTTCTGGAGAATTCTATCTCCTGCTTTCTTGATGAAAACCAAAACTAGTGGATTGATGATCACTCGTCCGATGATAGTCACGCTAATGGCACTTTCCCTACGGATCAACGTCTGGA
ATTTTGATATTCACGCTAATGGCACTCATAAATTTCAATCTTCGCAAAATTTCTTGCCTCAACTCTAGATCCAAATCAACCAATTAATATATTTTTTCAATTATGTTAATTGAGTGGAACTGTTGGCCAAAGAAATCACAGAACTTGGATATTCATGCTTTTACATCCATGCAAAGATGTTACGATCTCACCAGAACAAA
GTCTTCCATGACTTCCATAATGGTGCCTGTAGAAATCTAGTGTCTTCAGAAATACTAACAGGATGGAGAACAAAATGGACAAAACATCAACTAGTGATACACACAAAAAGATGAAAGGCTTGCTAAAAGTGAAGAATGCTTTCGCACAATCGTTCCAGTGTATCCGCACCAAATGTGAACAACTAATGATACATAGCTCA
TCAAATTGCATAGTTTGCAAAGAAGCAACATAAGTGAGATATGAGCACAATGTTGAATGCATTCTAGAAACAGAGGATGGGCCAATTAAATGTACCTTACGTAGTCGAGCACTTGAAGAATGTTTCCCAGAAATTGGGAAAATATCTTATGAGAATTACTCAAAAAAGATTTCGTCAAGTGTGTTGTGTTTGCAAAAACT
ATGCTAGACAGGCCTTTTCACACTGTCAGTAGGCAACAACATCACATAACTAAAAAAACTATGATCACTGTCTCTGAAGTCGTTGCAATGTTTTTTGTTGTCAAGGAACAATTACTTTTCCAAAGAATAAATTTGGATTAGTAGTATGGGTTGTTAGCAATTACTTTTCAGACTTCTTCTAACCTATTCTCCATAGTTTT
ATCTCTATTGAATCCATAACTTATATTAATGTTACTAAAGTTGCATAAGGGAATTTTGGTCTAAACCTGGAGCCTTCACAACTCTTTTTGAAGAGAAGGAAAATAGGGGGGGAATGCTGACAATACATCGATATTGTTGTTCAATCATACCTGCAGTTTTGTATTTCAATATCTG

>TRINITY_DN39070_c0_g1_i1 from sample A7 / Example unspliced isoform:

AATCTAAGCATTCTTTAAAAAAAACCACTTTTGTTGGCGACTTATGCCTTATATGGCAAAATGAAATGCAAACAACACTTTCCTGGGTATTCTGTTGTTGCTACTATTTTCTTTTAAAATTTTCCTCCAAATAGTATTTGTCAAGTTATGTTGTTGAGTTGAATGAATAGCCTTATGCATATTTATTTTTCTGCCTTTGA
ACTTGGTTTTCTTCCCATATGCTTCCTCTTCATCTATCAACATATTGTCTTGCTAGTAATTACAAAATATCAAACAATTTACCAAAATATCTATGCATTCCAGAAATACTAACAGGATGGAGAACAAAATGGACAAAACATCAACTAGTGATACACACAAAAAGgtgcattttttattattattcatcctatacaaatac
aagcaaattcccaaagttgctatatagtagattatggtcctaatacatatttatttttctcgttctttcttttgcctatttacaataatgcaactatgctcgatgatatataacaaagtttttgagggagacgtgttagcactatatggacatggccagagcaaagggccaaattcattgacaaaaacacaattagtaca
tgctgatctagtagacaaggatggacagatgacaataactgtatacatcaacaaccacttaatcccttgtttcattgattaattctgtcctggagttagtgtcagaatagtcgactttgtcttgaagaaaaaatgaccataccataaaggagatgcgaaattctgcattgtcctccttgatagaacaaaatttgagttta
ttgagcaggtttgcaaatatcacaaatttccaccggacaccataattgatgagttagtgcaatcaaatgatgcctattcagttggttctattgcagcaattgtcattggatgtcaataaaacaacacacaatacgatctaagtatcaaggatggagagatgccatagacaatgcaattgtaagtcttgaccatttttatc
aaaaatactttttctttgtagATGAAAGGCTTGCTAAAAGTGAAGAATGCTTTCGCACAATCGTTCCAGTGTATCCGCACCAAATGTGAACAACTAATGATACATAGCTCATCAAATTGCATAGTTTGCAAAGAAGCAACATAAGTGAGATATGAGCACAATGTTGAATGCATTCTAGAAACAGAGGATGGGCCAATTAA
ATGTACCTTACGTAGTCGAGCACTTGAAGAATGTTTCCCAGAAATTGGGAAAATATCTTATGAGAATTACTCAAAAAAGATTTCGTCAAGTGTGTTGTGTTTGCAAAAACTATGCTAGACAGGCCTTTTCACACTGTCAGTAGGCAACAACATCACATAACTAAAAAAACTATGATCACTGTCTCTGAAGTCGTTGCAAT
GTTTTTTGTTGTCAAGGAACAATTACTTTTCCAAAGAATAAATTTGGATTAGTAGTATGGGTTGTTAGCAATTACTTTTCAGACTTCTTCTAACCTATTCTCCATAGTTTTATCTCTATTGAATCCATAACTTATATTAATGTTACTAAAGTTGCATAAGGGAATTTTGGTCTAAACCTGGAGCCTTCACAACTCTTTTT
GAAGAGAAGGAAAATAGGGGGGGAATGCTGACAATACATCGATATTGTTGTTCAATCATACCTGCAGTTTTGTATTTCAATATCTG

Intron 4 GTTGGCTTTA ... TTTCCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.17	17	7	4.7
A2	19.23	21	5	6
A3	16.94	13	2.7	4.7
A4	28.57	5	2	1.3
A5	36.36	7	4	0.7
A6	24	47.5	15	9.3
A7	15.46	82	15	15.3
A8	23.36	82	25	21.7
A9	16.1	78.2	15	15
A10	32.69	35	17	5.3

>TRINITY_DN43305_c4_g1_i1 from sample A8 / Example spliced isoform

GTGAATTACTAAAAAAATGAGTAAATTAAAAAAAAGAATTGGAGGGAAAATAAATGAAGCTTAAATAGATCCAATCTGTCCGAGTTGAATGGATGCCAAAAAAGGGTTGAATCATGCCAAAACTGACAACCTGATCAAGAAATTCAGCATCTAGCTCTGTCCTGACAGTAAAGATCGTTGGGTGGGCTAACAATGCGGGT
AGTGCACACAGCTTACCCTGTAGGTCCATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGGTAAGTGGCTTTCCCCTTTTGGGTGCTTCCAACTTAGGGTTTGGCGTTCTTGTTGGAAGAACTTCCGAGTATGG
AAACATTGGTATCTTTAAAGGGAAGAACTTGCCTAGAGCGTGCTCCTAGTCCCAAACTTCTACAACGGGCTCAAGCTTTGGGCAAGCTATTACATCAAAATAGCCTTCTGGAGAATTCTATCTCCTGCTTTCTTGATGAAAACCAAAACTAGTGGATTGATGATCACTCGTCCGATGATAGTCACGCTAATGGCACTTTC
CCTACGGATCAACGTCTGGAATTTTGATATTCACGCTAATGGCACTCATAAATTTCAATCTTCGCAAAATTTCTTGCCTCAACTCTAGATCCAAATCAACCAATTAATATATTTTTTCAATTATGTTAATTGAGTGGAACTGTTGGCCAAAGAAATCACAGAACTTGGATATTCATGCTTTTACATCCATGCAAAGATGT
TACGATCTCACCAGAACAAAGTCTTCCATGACTTCCATAATGGTGCCTGTAGAAATCTAGTGTCTTCAGAAATACTAACAGGATGGAGAACAAAATGGACAAAACATCAACTAGTGATACACACAAAAAGATGAAAGGCTTGCTAAAAGTGAAGAATGCTTTCGCACAATCGTTCCAGTGTATCCGCACCAAATGTGAAC
AACTAATGATACATAGCTCATCAAATTGCATAGTTTGCAAAGAAGCAACATAAGTGAGATATGAGCACAATGTTGAATGCATTATAGAAACAGAGGATGGGCCAATTAAATGTACCTTACGTAGTCGAGCACTTGAAGAATGTTTCCCAGAAATTGGGAAAATATCTTATGAGAATTACTCAAAAAAGATTTCGTCAAGT
GTGTTGTGTTTGCAAAAACTATGCTAGACAGGCCTTTTCACACTGTCAGTAGGCAACAACATCACATAACTAAAAAAACTATGATCACTGTCTCTGAAGTCGTTGCAATGTTTTTTGTTGTCAAGGAACAATTACTTTTCCAAAGAATAAATTTGGATTAGTAGTATGGGTTGTTAGCAATTACTTTTCAGACTTCTTCT
AACCTATTCTCCATAGTTTTATCTCTATTGAATCCATAACTTATATTAATGTTACTAAAGTTGCATAAGGGAATTTTGGTCTAAACCTGGAGCCTTCACAACTCTTTTTGAAGAGAAGGAAAATAGGGGGGGAATGCTGACAATACATCGATATTGTTGTTCAATCATACCTGCAGTTTTGTATTTCAATATCTGTTACA
TTCCAAATGCAATTTAGAAGTTTTTTTCTGCCTAAAGTCTTTGAACATTTACATTCTATTATGTGTTAATATATAGCTTTGGTTCTTTGATCTCATAGTTGTCATTTATTGCAAGATAGATTCATATGAATTTGTGGAACACTATCAATTTCTTTCCCTAATGCAAACATAACCAATGATTGTTGAGAGCTATTTTTTTC
TTAGGATAACAAATCTTCTACAAGTAACAACAAGGACATGCCAGCTGATGAAAACATTCCACACACCTGTGAGCACCTACTAAATT

>TRINITY_DN43305_c4_g1_i8 from sample A8 / Example unspliced isoform:

CCTGACAGTAAAGATCGTTGGGTAAATATATATGAGGTGGGCTAACAATGCGGGTAGTGCACACAGCTTACCCTGTAGGTCCATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGGTAAGTGGCTTTCCCCTTT
TGGGTGCTTCCAACTTAGGGTTTGGCGTTCTTGTTGGAAGAACTTCCGAGTATGGAAACATTGGTATCTTTAAAGGGAAGAACTTGCCTAGAGCGTGCTCCTAGTCCCAAACTTCTACAACGGGCTCAAGCTTTGGGCAAGCTATTACATCAAAATAGCCTTCTGGAGAATTCTATCTCCTGCTTTCTTGATGAAAACCA
AAACTAGTGGATTGATGATCACTCGTCCGATGATAGTCACGCTAATGGCACTTTCCCTACGGATCAACGTCTGGAATTTTGATATTCACGCTAATGGCACTCTCATAAATTTCAATCTTCGCAAAATTTCTTGCCTCAACTCTAGgttggctttatcattttgcttcataaaagtgcgctcatttgttttgtttggatgt
gagctaaatcttaagcattattaaaagttttgttgttttcctgcagATCCAAATCAACCAATTAATATATTTTTTCAATTATGTTAATTGAGTGGAACTGTTGGCCAAAGAAATCACAGAACTTGGATATTCATGCTTTTACATCCATGCAAAGATGTTACGATCTCACCAGAACAAAGTCTTCCATGACTTCCATAATG
GTGCCTGTAGAAATCTAGTGTCTTCAGGTAAGCAAATATCTTGTAACCATCAAATGGCTTAAACGTCTTATATTCCTCTATTTTTTACTCTGTTTTTATCATTCACGATCTTATTGTTCATTACATTTATTGTCCCAGAAAACACTGCCTCTTTCCTCATGTATAAAGAAACATTGAAAATAATAATGATGGGTATATTT
ATATATCTATTGAAAACCTATGTTGGCTTTAGCATCTACGATTTGTTATTTGGCTGTGTATGTGCAATGAATATGGCACAATGTCCTACAATGAACATGGATTCCATGTATAGAGCCTACTACATTTGTTTCCTTATCGCAATTGGCATTATC

Intron 5 GTAAGTGGCT ... TCAACTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.16	11	28	17
A2	50.52	24	28	21
A3	51.02	24	37	13
A4	56.36	12	26	5
A5	62.79	8	20	7
A6	66.18	35	90	47
A7	59.12	65	106	82
A8	57.32	67	98	82
A9	60.74	64	119	79
A10	62.32	39	94	35

>TRINITY_DN101588_c0_g2_i1 from sample A2 / Example spliced isoform

AAAAAAAAGAATTGGAGGGAAAATAAATGAAGCTTAAATAGATCCAATCTGTCCGAGTTGAATGGATGCCAAAAAAGGGTTGAATCATGCCAAAACTGACAACCTGATCAAGAAATTCAGCATCTAGCTCTGTCCTGACAGTAAAGATCGTTGGGTGGGCTAACAATGCGGGTAGTGCACACAGCTTACCCTGTAGGTCC
ATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGATCCAAATCAACCAATTAATATATTTTTTCAATTATGTTAATTGAGTGGAACTGTTGGCCAAAGAAATCACAGAACTTGGATATTCATGCTTTTACATCCA
TGCAAAGATGTTACGATCTCACCAGAACAAAGTCTTCCATGACTTCCATAATGGTGCCTGTAGAAATCTAGTGTCTTCAGATGAAAGGCTTGCTAAAAGTGAAGAATGCTTTCGCACAATCGTTCCAGTGTATCCGCACCAAATGTGAACAACTAATGATACATAGCTCATCAAATTGCATAGTTTGCAAAGAAGCAACA
TAAGTGAGATATGAGCACAATGTTGAATGCATTATAGAAACAGAGGATGGGCCAATTAAATGTACCTTACGTAGTCGAGCACTTGAAGAATGTTTCCCAGAAATTGGGAAAATATCTTATGAGAATTACTCAAAAAAGATTTCGTCAAGTGTGTTGTGTTTGCAAAAACTATGCTAGACAGGCCTTTTCACACTGTCAGT
AGGCAACAACATCACATAACTAAAAAAACTATGATCACTGTCTCTGAAGTCGTTGCAATGTTTTTTTGTTGTCAAGGAACAATTACTTTTCCAAAGAATAAATTTGGATTAGTAGTATGGGTTGTTAGCAATTACTTTTCAGACTTCTTCTAA

>TRINITY_DN101588_c0_g2_i2 from sample A2 / Example unspliced isoform:

AAAAAAAAGAATTGGAGGGAAAATAAATGAAGCTTAAATAGATCCAATCTGTCCGAGTTGAATGGATGCCAAAAAAGGGTTGAATCATGCCAAAACTGACAACCTGATCAAGAAATTCAGCATCTAGCTCTGTCCTGACAGTAAAGATCGTTGGGTGGGCTAACAATGCGGGTAGTGCACACAGCTTACCCTGTAGGTCC
ATACGCGGGTATGCATTAACGCTGGAGACTTGGATTTGGGCTCTCGGTCTCGTAGGGTGTGGTATTTCCCGTGGGGCGTGGTGCAATTGTGCGCAGCTGgtaagtggctttccccttttgggtgcttccaacttagggtttggcgttcttgttggaagaacttccgagtatggaaacattggtatctttaaagggaagaa
cttgcctagtgcgtgctcctagtcccaaacttctacaacgggctcaagctttgggcaagctattacatcaaaatagccttctggagaattctatctcctgctttcttgatgaaaaccaaaactagtggattgatgatcactcgtccgatgatagtcacgctaatggcactttccctacggatcaacgtctggaattttga
tattcacgctaatggcactctcataaatttcaatctttgcaaaatttcttgcctcaactctagATCCAAATCAACCAATTAATATATTTTTTCAATTATGTTAATTGAGTGGAACTGTTGGCCAAAGAAATCACAGAACTTGGATATTCATGCTTTTACATCCATGCAAAGATGTTACGATCTCACCAGAACAAAGTCTT
CCATGACTTCCATAATGGTGCCTGTAGAAATCTAGTGTCTTCAGATGAAAGGCTTGCTAAAAGTGAAGAATGCTTTCGCACAATCGTTCCAGTGTATCCGCACCAAATGTGAACAACTAATGATACATAGCTCATCAAATTGCATAGTTTGCAAAGAAGCAACATAAGTGAGATATGAGCACAATGTTGAATGCATTATA
GAAACAGAGGATGGGCCAATTAAATGTACCTTACGTAGTCGAGCACTTGAAGAATGTTTCCCAGAAATTGGGAAAATATCTTATGAGAATTACTCAAAAAAGATTTCGTCAAGTGTGTTGTGTTTGCAAAAACTATGCTAGACAGGCCTTTTCACACTGTCAGTAGGCAACAACATCACATAACTAAAAAAACTATGATC
ACTGTCTCTGAAGTCGTTGCAATGTTTTTTTGTTGTCAAGGAACAATTACTTTTCCAAAGAATAAATTTGGATTAGTAGTATGGGTTGTTAGCAATTACTTTTCAGACTTCTTCTAA