sp|Q84JE8|ZDP_ARATH ----> Polynucleotide 3'-phosphatase ZDP

Intron 1 GTGATTCATA ... CTTATGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.63	38	8	27.5
A2	27.6	41.3	9	14
A3	35.01	69.3	14	31.5
A4	37.34	16.8	7	9
A5	40.44	18.8	2	11
A6	39.74	148.5	14.5	83.5
A7	34.57	161	24.5	77
A8	37.94	143	18.5	78
A9	35.43	204.5	35.5	98.5
A10	38.33	141	20	77

>TRINITY_DN115551_c0_g1_i7 from sample A1 / Example spliced isoform

GACGACTCAAAACAGTCCCATGCTTTACAAAGGAGCCCCGTGTGCTCTTCCCGCCGTCCTTACCAATTCCGCAATTCCTAACAATTTTTTTCCTAAAACCCTCTCCCGCGCCTGCTATACTCAGTCGCTATATCACCTTCTGGAAATTTCTGGCTCCAAACCTACTGACACATTTATTTCCGCAGGTCACAGGAGGCTTT
TGGCTGTTTCCTATCTGTTTAAGGTCTCACTTTGCGAGCAAATGCCTCCGAAATCGAAGATTTTAGTAGAGTATGCAAAGTCGGCCAGGGCAGGTTGCAAAAAATGCGGAAATACAATAGACAAGGGTTGCATGAGACTCGGGATTGCGAGTAAAGCAGCCGGCGGGTTCGATATGACCCGTTGGCATCATCTTCACTGC
TTTCCATCTCAGTCCGAGGTTGTTTCTGTTGAAAATATCAATGGATTTCAGCTGCTCAAAGCGTCTGATCAGGAAGCTTTGAAAACACTTGTAGCCGAGTTCGGGAAAAACCCGGGGTCTCCTAGGACAGGAAACGAGGATTCTCAACAAAAACCACTGAAATCAATCACAGAAGAAATGTGCGGCGACGATCAAAGAAA
TCCTAAGAAACTCAAATCAGAGAACACTTCAATTAAGTCAAGTGGACACAATGATTCGGGTTTTCTGGATATGTTTTCGCCATCTGAGCTTACAAGTAGATACAAGGATGCTATTCTACCTCCAAAATGGAAGGCATTTCAAACAGTCATTTTTCTTGGGCAGGATGATGGTCTGGAGGCATCAGAAAAGATTGCTGCAT
TTGATTTTGATGGTTGTCTTGTCAGAACTTCTGTCAGGAAAGTTGGAGCAGATGCCTGGTCCCTCCACTATTCTTCCATTCCTGAAAAGTTGCAGAGTTACTACAATAAAGGGTACAAACTGGTGATCTTCACAAATGAGTCGAACATTGATCGGTGGAAAAATAGTAGGCAAAAGGCAATAGACTCGAAGCTTGGACGG
TTAGAAGGATTCATTAAACGAGTAAAGGTGCCAATGCAGGTCTTTGTAGCTTGTGGGATAGAAGGAACAGGTGATTCATTCAGGAAACCAAAGTCAGGAATGTGGAGGTTAATGGAGCGGCATTTAAATAACGGAATCGTGATTGACATGGAACAGTCTTTTTATGTTGGAGATGCAGCTGGGAGAATCAACGACCACAG
TGATGCAGATATTGGTTTTGCTAAGGATGTTGGTTTGAGGTTTCTTTTGCCTGAAGATTTTTTCGCTCCAGAAGCTTGAGAGTTGTCTTTCGCTTGTTTGATATTGCACTATTCGGACCTGGAGATGCGGCCATGACCATTTGAGTTAACATCTCGATACCGTTGATCGTAGTTCAACTAGAATTGGGAATCACTAGCAT
GTCACTATCAAGAAGAATACTGTCAGATTTTCATCTCTGTTACAAAATACAGAGATACAGTTTTGTCTGCAAGAAGTGTTGAGCAGTTTTTCCTTCTGCCATTGTCAGAAGGAAAACCTTTTCTCCTAATTAAAACTGAGACTTGGGCATGGAAGGTTGTAATCGTGACCATTTAAATGTTTGGGTTGCATTAAATTCAT
TTTGTGCAGCTTCACCACATTAAGTTGTTTCTAGGGTCTGATGTTTAAGGAAAATTTATGTTTTTTAAAATGATAATGACAATATTTTGATATGAATAGGCAGATGTATTTCTTTATCATATATAGTATATCGCATTAAAATGGTCAGATGCCATTTCATTGCTGTTCAGATCTTGTTCCTGCC

>TRINITY_DN115551_c0_g1_i3 from sample A1 / Example unspliced isoform:

TGCAAAGGTTATGTTAATAAATCAGAAGTGAAGTCTACTGCTTTCAGGATTTTACTTTTGAGTCTCAGCTTTTTGTTTATACATGTTCTTGCTACTCAGTATTGTTTGGGTTTTCAGATTTCGTTTGCTGAAGGTATATAACTGTCATGGTCAGTTAAATGTTAGTTTTATTTGATGGGATGGAAAGCAGGTGATCTTCA
CAAATGAGTCGAACATTGATCGGTGGAAAAATAGTAGGCAAAAGGCAATAGACTCGAAGCTTGGACGGTTAGAAGGATTCATTAAACGAGTAAAGGTGCCAATGCAGgtgattcatatgctttgttactgtcttcaaactgccgttatttaatatgttatgctttctattatagatagatatttaggacgatcattactg
tttgttggatatatatatttcaatgttaaaattcagatgcaatgatgcttgcttatgtcagGTCTTTGTAGCTTGTGGGATAGAAGGAACAGGTGATTCATTCAGGAAACCAAAGTCAGGAATGTGGAGGTTAATGGAGCGGCATTTAAATAACGGAATCGTGATTGACATGGAACAGATGTTGGTTTGAGGTTTCTTTT
GCCTGAAGATTTTTTCGCTCCAGAAGCTTGAGAGTTGTCTTTCGCTTGTTTGATATTGCACTATTCGGACCTGGAGATGCGGCCATGACCATTTGAGTTAACATCTCGATACCGTTGATCGTAGTTCAACTAGAATTGGGAATCACTAGCATGTCACTATCAAGAAGAATACTGTCAGATTTTCATCTCTGTTACAAAAT
ACAGAGATACAGTTTTGTCTGCAAGAAGTGTTGAGCAGTTTTTCCTTCTGCCATTGTCAGAAGGAAAACCTTTTCTCCTAATTAAAACTGAGACTTGGGCATGGAAGGTTGTAATCGTGACCATTTAAATGTTTGGGTTGCATTAAATTCATTTTGTGCAGCTTCACCACATTAAGTTGTTTCTAGGGTCTGATGTTTAA
GGAAAATTTATGTTTTTTAAAATGATAATGACAATATTTTGATATGAATAGGCAGATGTATTTCTTTATCATATATAGTATATCGCATTAAAATGGTCAGATGCCATTTCATTGCTGTTCAGATCTTGTTCCTGCC

Intron 2 GCGTCTGATC ... AGGAAACGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	85.48	9	46	60
A2	79.37	13	54	46
A3	75.46	20	69	54
A4	66.67	19	37	39
A5	68.42	12	26	26
A6	67.12	36	85	62
A7	73.29	37	116	87
A8	82.35	18	92	76
A9	68.8	39	90	82
A10	68.5	40	92	82

>TRINITY_DN36207_c0_g1_i4 from sample A9 / Example spliced isoform

GGAATTACCGAGCAGAAAGGCACTGAAAGGGCCAATGCTCAGATGACCGTTCACTTGACGACTCAAAACAGTCCCATGCTTTACAAAGGAGCCCCGTGTGCTCTTCCCGCCGTCCTTACCAATTCCGCAATTCCTAACAATTTTTTTCCTAAAACCCTCTCCCGCGCCTGCTATACTCAGTCGCTATATCACCTTCTGGA
AATTTCTGGCTCCAAACCTACTGACACATTTATTTCCGCAGGTCACAGGAGGCTTTTGGCTGTTTCCTATCTGTTTAAGGTCTCACTTTGCGAGCAAATGCCTCCGAAATCGAAGATTTTAGTAGAGTATGCAAAGTCGGCCAGGGCAGGTTGCAAAAAATGCGGAAATACAATAGACAAGGGTTGCATGAGACTCGGGA
TTGCGAGTAAAGCAGCCGGCGGGTTCGATATGACCCGTTGGCATCATCTTCACTGCTTTCCATCTCAGTCCGAGGTTGTTTCTGTTGAAAATATCAATGGATTTCAGCTGCTCAAAGATTCTCAACAAAAACCACTGAAATCAATCACAGAAGAAATGTGCGGCGACGATCAAAGAAATCCTAAGAAACTCAAATCAGAG
AACACTTCAATTAAGTCAAGTGGACACAATGATTCGGGTTTTCTGGATATGTTTTCGCCATCTGAGCTTACAAGTAGATACAAGGATGCTATTCTACCTCCAAAATGGAAGGCATTTCAAACAGTCATTTTTCTTGGGCAGGATGATGGTCTGGAGGCATCAGAAAAGATTGCTGCATTTGATTTTGATGGTTGTCTTGT
CAGAACTTCTGTCAGGAAA

>TRINITY_DN36207_c0_g1_i3 from sample A9 / Example unspliced isoform:

GGAATTACCGAGCAGAAAGGCACTGAAAGGGCCAATGCTCAGATGACCGTTCACTTGACGACTCAAAACAGTCCCATGCTTTACAAAGGAGCCCCGTGTGCTCTTCCCGCCGTCCTTACCAATTCCGCAATTCCTAACAATTTTTTTCCTAAAACCCTCTCCCGCGCCTGCTATACTCAGTCGCTATATCACCTTCTGGA
AATTTCTGGCTCCAAACCTACTGACACATTTATTTCCGCAGGTCACAGGAGGCTTTTGGCTGTTTCCTATCTGTTTAAGGTCTCACTTTGCGAGCAAATGCCTCCGAAATCGAAGATTTTAGTAGAGTATGCAAAGTCGGCCAGGGCAGGTTGCAAAAAATGCGGAAATACAATAGACAAGGGTTGCATGAGACTCGGGA
TTGCGAGTAAAGCAGCCGGCGGGTTCGATATGACCCGTTGGCATCATCTTCACTGCTTTCCATCTCAGTCCGAGGTTGTTTCTGTTGAAAATATCAATGGATTTCAGCTGCTCAAAgcgtctgatcaggaagctttgaaaacacttgtagccgagttcgggaaaaacccggggtctcctaggacaggaaacgagGATTCT

CAACAAAAACCACTGAAATCAATCACAGAAGAAATGTGCGGCGACGATCAAAGAAATCCTAAGAAACTCAAATCAGAGAACACTTCAATTAAGTCAAGTGGACACAATGATTCGGGTTTTCTGGATATGTTTTCGCCATCTGAGCTTACAAGTAGATACAAGGATGCTATTCTACCTCCAAAATGGAAGGCATTTCAAAC
AGTCATTTTTCTTGGGCAGGATGATGGTCTGGAGGCATCAGAAAAGATTGCTGCATTTGATTTTGATGGTTGTCTTGTCAGAACTTCTGTCAGGAAAGTTGGAGCAGATGCCTGGTCCCTCCACTATTCTTCCATTCCTGAAAAGTTGCAGAGTTACTACAATAAAGGGTACAAACTGGTGATCTTCACAAATGAGTCGA
ACATTGATCGGTGGAAAAATAGTAGGCAAAAGGCAATAGACTCGAAGCTTGGACGGTTAGAAGGATTCATTAAACGAGTAAAGGTGCCAATGCAGGTCTTTGTAGCTTGTGGGATAGAAGGAACAGGTGATTCATTCAGGAAACCAAAGTCAGGAATGTGGAGGTTAATGGAGCGGCATTTAAATAACGGAATCGTGATT
GACATGGAACAGTATGTTTTATAATTTATGGAGCATACTTTTTGGTTGCTTGATTGAAATTCACAATACCAATAATGGTTATATTTTCAAAAATTGGAAAAGAGTAAATTATTGTTTGCTTCAAAGGGCTGTAAGTTATATTGAGTACCAGAAAACACAGGAAAATATTGCTTATAATTTGGGGGCTTTGGGGAAAGTAT
TTTGCAACAGAGGCACTGTGCTGAATGATGCTTTTTGTATACATAAATGTTACCAAGTTATCCTGTGGCA

Intron 3 GTCTTTTTAT ... TTTTGCTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.92	23	28.3	33
A2	66.63	13	19	28.7
A3	70.32	16.7	46.7	22
A4	73.74	10	20.7	35
A5	82.61	2	7	8.7
A6	63.68	106	165	199
A7	59.53	80	121.3	105
A8	60.95	105.3	158	159
A9	59.43	109.7	161.3	143
A10	67.16	57.7	117.3	89

>TRINITY_DN115551_c0_g1_i3 from sample A1 / Example spliced isoform

TGCAAAGGTTATGTTAATAAATCAGAAGTGAAGTCTACTGCTTTCAGGATTTTACTTTTGAGTCTCAGCTTTTTGTTTATACATGTTCTTGCTACTCAGTATTGTTTGGGTTTTCAGATTTCGTTTGCTGAAGGTATATAACTGTCATGGTCAGTTAAATGTTAGTTTTATTTGATGGGATGGAAAGCAGGTGATCTTCA
CAAATGAGTCGAACATTGATCGGTGGAAAAATAGTAGGCAAAAGGCAATAGACTCGAAGCTTGGACGGTTAGAAGGATTCATTAAACGAGTAAAGGTGCCAATGCAGGTGATTCATATGCTTTGTTACTGTCTTCAAACTGCCGTTATTTAATATGTTATGCTTTCTATTATAGATAGATATTTAGGACGATCATTACTG
TTTGTTGGATATATATATTTCAATGTTAAAATTCAGATGCAATGATGCTTGCTTATGTCAGGTCTTTGTAGCTTGTGGGATAGAAGGAACAGGTGATTCATTCAGGAAACCAAAGTCAGGAATGTGGAGGTTAATGGAGCGGCATTTAAATAACGGAATCGTGATTGACATGGAACAGATGTTGGTTTGAGGTTTCTTTT
GCCTGAAGATTTTTTCGCTCCAGAAGCTTGAGAGTTGTCTTTCGCTTGTTTGATATTGCACTATTCGGACCTGGAGATGCGGCCATGACCATTTGAGTTAACATCTCGATACCGTTGATCGTAGTTCAACTAGAATTGGGAATCACTAGCATGTCACTATCAAGAAGAATACTGTCAGATTTTCATCTCTGTTACAAAAT
ACAGAGATACAGTTTTGTCTGCAAGAAGTGTTGAGCAGTTTTTCCTTCTGCCATTGTCAGAAGGAAAACCTTTTCTCCTAATTAAAACTGAGACTTGGGCATGGAAGGTTGTAATCGTGACCATTTAAATGTTTGGGTTGCATTAAATTCATTTTGTGCAGCTTCACCACATTAAGTTGTTTCTAGGGTCTGATGTTTAA
GGAAAATTTATGTTTTTTAAAATGATAATGACAATATTTTGATATGAATAGGCAGATGTATTTCTTTATCATATATAGTATATCGCATTAAAATGGTCAGATGCCATTTCATTGCTGTTCAGATCTTGTTCCTGCC

>TRINITY_DN115551_c0_g1_i7 from sample A1 / Example unspliced isoform:

GACGACTCAAAACAGTCCCATGCTTTACAAAGGAGCCCCGTGTGCTCTTCCCGCCGTCCTTACCAATTCCGCAATTCCTAACAATTTTTTTCCTAAAACCCTCTCCCGCGCCTGCTATACTCAGTCGCTATATCACCTTCTGGAAATTTCTGGCTCCAAACCTACTGACACATTTATTTCCGCAGGTCACAGGAGGCTTT
TGGCTGTTTCCTATCTGTTTAAGGTCTCACTTTGCGAGCAAATGCCTCCGAAATCGAAGATTTTAGTAGAGTATGCAAAGTCGGCCAGGGCAGGTTGCAAAAAATGCGGAAATACAATAGACAAGGGTTGCATGAGACTCGGGATTGCGAGTAAAGCAGCCGGCGGGTTCGATATGACCCGTTGGCATCATCTTCACTGC
TTTCCATCTCAGTCCGAGGTTGTTTCTGTTGAAAATATCAATGGATTTCAGCTGCTCAAAGCGTCTGATCAGGAAGCTTTGAAAACACTTGTAGCCGAGTTCGGGAAAAACCCGGGGTCTCCTAGGACAGGAAACGAGGATTCTCAACAAAAACCACTGAAATCAATCACAGAAGAAATGTGCGGCGACGATCAAAGAAA
TCCTAAGAAACTCAAATCAGAGAACACTTCAATTAAGTCAAGTGGACACAATGATTCGGGTTTTCTGGATATGTTTTCGCCATCTGAGCTTACAAGTAGATACAAGGATGCTATTCTACCTCCAAAATGGAAGGCATTTCAAACAGTCATTTTTCTTGGGCAGGATGATGGTCTGGAGGCATCAGAAAAGATTGCTGCAT
TTGATTTTGATGGTTGTCTTGTCAGAACTTCTGTCAGGAAAGTTGGAGCAGATGCCTGGTCCCTCCACTATTCTTCCATTCCTGAAAAGTTGCAGAGTTACTACAATAAAGGGTACAAACTGGTGATCTTCACAAATGAGTCGAACATTGATCGGTGGAAAAATAGTAGGCAAAAGGCAATAGACTCGAAGCTTGGACGG
TTAGAAGGATTCATTAAACGAGTAAAGGTGCCAATGCAGGTCTTTGTAGCTTGTGGGATAGAAGGAACAGGTGATTCATTCAGGAAACCAAAGTCAGGAATGTGGAGGTTAATGGAGCGGCATTTAAATAACGGAATCGTGATTGACATGGAACAgtctttttatgttggagatgcagctgggagaatcaacgaccacag
tgatgcagatattggttttgctaagGATGTTGGTTTGAGGTTTCTTTTGCCTGAAGATTTTTTCGCTCCAGAAGCTTGAGAGTTGTCTTTCGCTTGTTTGATATTGCACTATTCGGACCTGGAGATGCGGCCATGACCATTTGAGTTAACATCTCGATACCGTTGATCGTAGTTCAACTAGAATTGGGAATCACTAGCAT
GTCACTATCAAGAAGAATACTGTCAGATTTTCATCTCTGTTACAAAATACAGAGATACAGTTTTGTCTGCAAGAAGTGTTGAGCAGTTTTTCCTTCTGCCATTGTCAGAAGGAAAACCTTTTCTCCTAATTAAAACTGAGACTTGGGCATGGAAGGTTGTAATCGTGACCATTTAAATGTTTGGGTTGCATTAAATTCAT
TTTGTGCAGCTTCACCACATTAAGTTGTTTCTAGGGTCTGATGTTTAAGGAAAATTTATGTTTTTTAAAATGATAATGACAATATTTTGATATGAATAGGCAGATGTATTTCTTTATCATATATAGTATATCGCATTAAAATGGTCAGATGCCATTTCATTGCTGTTCAGATCTTGTTCCTGCC