NA ----> NA

Intron 1 GTAAGTTGGT ... CTGTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.8	25.6	26	20.8
A2	30.61	18	10	5.9
A3	47.15	11.8	10	11
A4	26.67	11	4	4
A5	35.29	11	5	7
A6	39.38	93.8	51	70.9
A7	33.61	80	34	47
A8	32.15	82.6	35	43.2
A9	35.01	95.2	45	57.6
A10	37.95	58.9	34	38

>TRINITY_DN121682_c0_g3_i10 from sample A1 / Example spliced isoform

GTTAATGAATGAGAAGTTTGTGCAAAAAATTTATGTTGAAGGAAGGATAATCAGTAGTGACTATTGTTTGAAGCAAGTGGACCGAAAGGCGACGCTGGTAATTGAGAAGGGCGCATTCCCTGTCAAGAAGGGATATACACCAGCCTCATCTTCAGGTCTGAAGTTTGGAGAAGAATACTGCCAGTATAAAATATTATCTC
TGCAGCTGTCCAAGAAAAGGGGTCGCAGTCTCTCCTTTTTACGAGTGAACTTGTGGTCTGTGGAAGAAGGGGAAGTTTACATCAGTTTATTGCATAGTCGTGGCTGTTAGTTGGGTGCACTGTCGTTTGAGAAGGGCGCGGCCTTGAAACTTCTCATTTGGGAGGTCGAATCTCTCCGTAATTACCCATTTACAGATACA
AAAGGGGCGTGTCCAGGCCTTAAGGAGGGCGAATCCACTGTAGGTAAAGTTGTTGCTGATTAGTAGTATTTCTAGCTTCAGGAGATCGCACCAGACTGCCGCACATCACTTATTCATGTATCAGCCATCTCTTCCACGTTTTTGACTGCTGGTCCAGACATCAAGCTTGCAAATCGAGTTCCACAGTGGCTGCTAATTAT
CACTAAATCGTGGCCTTCAAAGGGAGCCTTAACTCCGCATCATGGCCAGGTCACATGTAGATGATTATTTATTTTGCAATCTCTGTGGGAGCCTTCTATATTTTGATAATCCATTATTTGCATCTTGCCCTTTGTGTCATAATCAACACAATGTTAAAGATATTCGAGGGAAAGAGATATGGTGCAAGATTGGTGCTGAG

>TRINITY_DN121682_c0_g3_i13 from sample A1 / Example unspliced isoform:

GTTAATGAATGAGAAGTTTGTGCAAAAAATTTATGTTGAAGGAAGGATAATCAGTAGTGACTATTGTTTGAAGCAAGTGGACCGAAAGGCGACGCTGGTAATTGAGAAGGGCGCATTCCCTGTCAAGAAGGGATATACACCAGCCTCATCTTCAGGTCTGAAGTTTGGAGAAGAATACTGCCAGTATAAAATATTATCTC
TGCAGCTGTCCAAGAAAAGGGGTCGCAGTCTCTCCTTTTTACGAGTGAACTTGTGGTCTGTGGAAGAAGGGGAAGTTTACATCAGTTTATTGCATAGTCGTGGCTGTTAGTTGGGTGCACTGTCGTTTGAGAAGGGCGCGGCCTTGAAACTTCTCATTTGGGAGGTCGAATCTCTCCGTAATTACCCATTTACAGATACA
AAAGGGGCGTGTCCAGGCCTTAAGGAGGGCGAATCCACTGTAGGTAAAGTTGTTGCTGATTAGTAGTATTTCTAGCTTCAGGAGATCGCACCAGACTGCCGCACATCACTTATTCATGTATCAGCCATCTCTTCCACGTTTTTGACTGCTGGTCCAGACATCAAGCTTGCAAATCGAGTTCCACAGTGGCTGCTAATTAT
CACTAAATCGTGGCCTTCAAAGGGAgtaagttggtcattaaaggccgaccgtcagactctgtttacagGCCTTAACTCCGCATCATGGCCAGGTCACATGTAGATGATTATTTATTTTGCAATCTCTGTGGGAGCCTTCTATATTTTGATAATCCATTATTTGCATCTTGCCCTTTGTGTCATAATCAACACAATGTTAA
AGATATTCGAGGGAAAGAGATATGGTGCAAGATTGGTGCTGAG

Intron 2 GTATTATTTG ... TTTCTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	51.52	16	7	17
A2	78.57	3	0	11
A3	63.64	4	6	7
A4	53.85	6	3	7
A5	55	9	4	11
A6	64.79	50	11	92
A7	47.52	53	18	48
A8	53.68	44	10	51
A9	43.97	79	24	62
A10	56.1	36	11	46

>TRINITY_DN121682_c0_g3_i1 from sample A1 / Example spliced isoform

GAAGGTTTTTCCCGAGCAAGTTTTTGGAATAAGATTTGGATGCAGGTTTCTTCCGGTTAAGGGTTAACCAACGCCGAGGGAGAAATTCCTTTATTATATCTTAGGTAGATGGATTTGTTATTTCCAAGTTAGAAGAGAGGCATTTAAATTTGTAGTTTCTCATTTGGGATTGTCTCTCATTGAATTTGAGGTGATGAACA
TGAAGGCCGCTCGAATAAATGACAATAGCTTTAAGTCCACTTGAGGAAATTGCAGGCCTTAACTCCGCATCATGGTCAGGTCACATGTAGATGATTATTTATTTTGCAATCTCTGTGGGAGCCTTCTATATTTTGATAATCCATTATTTGCATCTTGCCCTTTGTGTCATAATCAACACAATGTTAAAGATATTCGAGGG
AAAGAGATATGGTGCAAGATTGGTGCTGAGATTCGACAGCAAGGCTGTTCCATCCACATTCCCACATATTTGATGGACAAGTTGAGATAAGGAGATCCTCTTACAACCAAGACATCATGATCGAATATGAAGATGAGAAACTTTTGAAGCTTAAAGGAACATCTGCAAGAGCTCAAAATTGTGCCTACCTCTCTCGGTAT
GAAGAAGGACCTAAGAAAACATCTTAATCTCGAGCCTTTTATAAAAGTGGACAATACCAAGGAAAGGCCAGAAGAGGATGAGATCCAGCAAAGGGCAGTGGTCAATGAAGACTGTCCTCAATGTAATCATCCACAGTTAGAGTATTATACTAGACAGTTACGGTCTGCAGATGAAGGACAAACAGTGTTTTATGAGTGTC
CAAACTGCCACCATAAATTTGCACAGAACACTTGAATCAGCCATGCCATACTTCTATGAGCATGTTTCCTATTATAGTAGATGACTTCTATTAACATTTTTTTGGGTCGAAACTCTGGGCTATGATGCAGTAAGCCTTTCAATATTTAAAAAAAAATTGTGTAGGGTGATTTTTACATAACCTTTAGAAGGGAATGTTGT
TTACAACATGTCTCTTTTATTTCAGTACTTTTGCTTTGCCCACAGCCCCATTGCAGCGTGTTTTGATGTTAGATTGAATCAAGGCATTCTTTTTATTCCTTAACATCCATTCTTAAAATAGAGATTCCATGCTGAAAATATTGTTGGGAATCAATTATTTGTTAAATTGCATATTAAGGATTTGTGACTCTTTGTGCATC
AACATGGCACTTTGAACACCGGTGTAACTATTTTTATTCAGTTTACAACTCTTTTTGCATCA

>TRINITY_DN121682_c0_g3_i5 from sample A1 / Example unspliced isoform:

TAGATGGATTTGTTATTTCCAAGTTAGAAGAGAGGCATTTAAATTTGTAGTTTCTCATTTGGGATTGTCTCTCATTGAATTTGAGGTGATGAACATGAAGGCCGCTCGAATAAATGAACAATAGCTTTAAGTCCACTTGAGGAAATTGCAGgtattatttgaaaccatttatatacattcttagattttttcgttttgta
gaaagaacaggctattaagactcctatatctggatgtagacatttatgggttcctgtgacttattggggaggaaaaagggctacgtttttctcacagGCCTTAACTCCGCATCATGGCCAGGTCACATGTAGATGATTATTTATTTTGCAATCTCTGTGGGAGCCTTCTATATTTTGATAATCCATTATTTGCATCTTGC
CCTTTGTGTCATAATCAACACAATGTTAAAGATATTCGAGGGAAAGAGATATGGTGCAAGATTGGTGCTGAG

Intron 3 GTAGATGGAT ... GAACATGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.44	10.1	15	22.3
A2	47.78	11.3	5	15.6
A3	74.38	3.4	3.6	16.8
A4	78.22	2.5	3	14
A5	82.67	3	6.3	21.9
A6	69.92	28.6	48	83.8
A7	62.84	35.6	21	100.2
A8	68.66	15	20.3	44
A9	72.07	27.5	35	100
A10	65.41	16.4	15.3	46.8

>TRINITY_DN121682_c0_g3_i7 from sample A1 / Example spliced isoform

GCAAGTTTTTGGAATAAGATTTGCAGGTTTCTTCCGGTTAAGGGTTAACCAACGCCGAGGGAGAAATTCCTTTATTATATCTTAGGCCGCTCGAATAAATGACAATAGCTTTAAGTCCACTTGAGGAAATTGCAGACATTTATGGGTTCCTGTGACTTATTGGGGAGGAAAAAGGGCTACGTTTTTCTCACAGGTATGAC
ATGGAATACTGTCCTGCGGCTCTGCGAGTAGTCATTGCATTCATATAATATATTGAGGCTTTAATTTAGTACTGAGTGTTTTTTTTGTAAACTTTTAGTTTAAAAGAGTCACGTGAACATGCAATTAAGTCATGTAAATAATGCTTTACATTATCA

>TRINITY_DN121682_c0_g3_i1 from sample A1 / Example unspliced isoform:

GAAGGTTTTTCCCGAGCAAGTTTTTGGAATAAGATTTGGATGCAGGTTTCTTCCGGTTAAGGGTTAACCAACGCCGAGGGAGAAATTCCTTTATTATATCTTAGgtagatggatttgttatttccaagttagaagagaggcatttaaatttgtagtttctcatttgggattgtctctcattgaatttgaggtgatgaaca
tgaagGCCGCTCGAATAAATGACAATAGCTTTAAGTCCACTTGAGGAAATTGCAGGCCTTAACTCCGCATCATGGTCAGGTCACATGTAGATGATTATTTATTTTGCAATCTCTGTGGGAGCCTTCTATATTTTGATAATCCATTATTTGCATCTTGCCCTTTGTGTCATAATCAACACAATGTTAAAGATATTCGAGGG
AAAGAGATATGGTGCAAGATTGGTGCTGAGATTCGACAGCAAGGCTGTTCCATCCACATTCCCACATATTTGATGGACAAGTTGAGATAAGGAGATCCTCTTACAACCAAGACATCATGATCGAATATGAAGATGAGAAACTTTTGAAGCTTAAAGGAACATCTGCAAGAGCTCAAAATTGTGCCTACCTCTCTCGGTAT
GAAGAAGGACCTAAGAAAACATCTTAATCTCGAGCCTTTTATAAAAGTGGACAATACCAAGGAAAGGCCAGAAGAGGATGAGATCCAGCAAAGGGCAGTGGTCAATGAAGACTGTCCTCAATGTAATCATCCACAGTTAGAGTATTATACTAGACAGTTACGGTCTGCAGATGAAGGACAAACAGTGTTTTATGAGTGTC
CAAACTGCCACCATAAATTTGCACAGAACACTTGAATCAGCCATGCCATACTTCTATGAGCATGTTTCCTATTATAGTAGATGACTTCTATTAACATTTTTTTGGGTCGAAACTCTGGGCTATGATGCAGTAAGCCTTTCAATATTTAAAAAAAAATTGTGTAGGGTGATTTTTACATAACCTTTAGAAGGGAATGTTGT
TTACAACATGTCTCTTTTATTTCAGTACTTTTGCTTTGCCCACAGCCCCATTGCAGCGTGTTTTGATGTTAGATTGAATCAAGGCATTCTTTTTATTCCTTAACATCCATTCTTAAAATAGAGATTCCATGCTGAAAATATTGTTGGGAATCAATTATTTGTTAAATTGCATATTAAGGATTTGTGACTCTTTGTGCATC
AACATGGCACTTTGAACACCGGTGTAACTATTTTTATTCAGTTTACAACTCTTTTTGCATCA

Intron 4 GTATTATTTG ... CTGGATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40.47	19.3	7	15
A2	14.21	13.3	0	4
A3	28.81	16.3	6	2
A4	25.5	10.8	3	2
A5	26.25	13.3	4	2
A6	29.81	61	11	27
A7	15.68	89.8	18	8
A8	29.68	36.8	10	13
A9	23.94	76	24	7
A10	21.25	46.3	11	9

>TRINITY_DN121682_c0_g3_i12 from sample A1 / Example spliced isoform

GAAGGTTTTTCCCGAGCAAGTTTTTGGAATAAGATTTGGATGCAGGTTTCTTCCGGTTAAGGGTTAACCAACGCCGAGGGAGAAATTCCTTTATTATATCTTAGGTAGATGGATTTGTTATTTCCAAGTTAGAAGAGAGGCATTTAAATTTGTAGTTTCTCATTTGGGATTGTCTCTCATTGAATTTGAGGTGATGAACA
TGAAGGCCGCTCGAATAAATGACAATAGCTTTAAGTCCACTTGAGGAAATTGCAGACATTTATGGGTTCCTGTGACTTATTGGGGAGGAAAAGGGCTACGTTTTTCTCACAGGCCTTAACTCCGCATCATGGTCAGGTCACATGTAGATGATTATTTATTTTGCAATCTCTGTGGGAGCCTTCTATATTTTGATAATCCA
TTATTTGCATCTTGCCCTTTGTGTCATAATCAACACAATGTTAAAGATATTCGAGGGAAAGAGATATGGTGCAAGATTGGTGCTGAGGACCTAAGAAAACATCTTAATCTCGAGCCTTTTATAAAAGTGGACAATACCAAGGAAAGGCCAGAAGAGGATGAGATCCAGCAAAGGGCAGTGGTCAATGAAGACTGTCCTCA
ATGTAATCATCCACAGTTAGAGTATTATACTAGACAGTTACGGTCTGCAGATGAAGGACAAACAGTGTTTTATGAGTGTCCAAACTGCCACCATAAATTTGCACAGAACACTTGAATCAGCCATGCCATACTTCTATGAGCATGTTTCCTATTATAGTAGATGACTTCTATTAACATTTTTTTGGGTCGAAACTCTGGGC
TATGATGCAGTAAGCCTTTCAATATTTAAAAAAAAATTGTGTAGGGTGATTTTTACATAACCTTTAGAAGGGAATGTTGTTTACAACATGTCTCTTTTATTTCAGTACTTTTGCTTTGCCCACAGCCCCATTGCAGCGTGTTTTGATGTTAGATTGAATCAAGGCATTCTTTTTATTCCTTAACATCCATTCTTAAAATA
GAGATTCCATGCTGAAAATATTGTTGGGAATCAATTATTTGTTAAATTGCATATTAAGGATTTGTGACTCTTTGTGCATCAACATGGCACTTTGAACACCGGTGTAACTATTTTTATTCAGTTTACAACTCTTTTTGCATCA

>TRINITY_DN121682_c0_g3_i5 from sample A1 / Example unspliced isoform:

TAGATGGATTTGTTATTTCCAAGTTAGAAGAGAGGCATTTAAATTTGTAGTTTCTCATTTGGGATTGTCTCTCATTGAATTTGAGGTGATGAACATGAAGGCCGCTCGAATAAATGAACAATAGCTTTAAGTCCACTTGAGGAAATTGCAGgtattatttgaaaccatttatatacattcttagattttttcgttttgta
gaaagaacaggctattaagactcctatatctggatgtagACATTTATGGGTTCCTGTGACTTATTGGGGAGGAAAAAGGGCTACGTTTTTCTCACAGGCCTTAACTCCGCATCATGGCCAGGTCACATGTAGATGATTATTTATTTTGCAATCTCTGTGGGAGCCTTCTATATTTTGATAATCCATTATTTGCATCTTGC
CCTTTGTGTCATAATCAACACAATGTTAAAGATATTCGAGGGAAAGAGATATGGTGCAAGATTGGTGCTGAG