NA ----> NA

Intron 1 GTAAACTGTT ... GGAGCTGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.67	2	20	6
A2	81.82	6	39	15
A3	93.1	1	23	4
A4	100	0	17	0
A5	91.3	1	14	7
A6	68.75	10	18	26
A7	53.7	25	25	33
A8	67.57	18	32	43
A9	63.22	16	26	29
A10	65.85	7	18	9

>TRINITY_DN40809_c0_g3_i4 from sample A6 / Example spliced isoform

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATT
GGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTT
CTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATTGATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

>TRINITY_DN40809_c0_g3_i1 from sample A6 / Example unspliced isoform:

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGgtaaactgttttctttttgttctgatattgtttctctttcagcaatcccaatatgtgctgaatataatgtgttatgaatttgttttggtttgcatggatga
tttgtgagggatcacctgtggtagtgtatggatcccaaagtttcttccaaatacgcttccagcaagagttatcaatatttccatgataggatggcttgaaccaaggctacgactatgcaagtggcctccgcgatgacaaaaacatatattataaagtagttccaggatcttattctactctctctctcttaatttataca
ggtgatataaattgtgtcatcaatgttataatgggaatactaggaatttattcagttaggctctggtcaataaaatcatcattggactctcctccatgggttcgacctaccttgggttttttctgaatccggggaaagggaggaaaaaactccagccacaatagcggttggtaatgtaacgggactcagccaggctagaa
ctggagacctcgtaaaaaaacatcgttctacagcattgatttgaagattctaaatcaagagaagagaagagcaatgagggctattgctggagctggagTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTG
GGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTC
TTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATTGATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

Intron 2 GTAGTTCCAG ... GTTGTGTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.67	14.7	33	10.3
A2	62.98	16.7	40	16.3
A3	61.12	7.7	19	5
A4	67.74	5	15	6
A5	49.56	16.3	22	9.7
A6	64.08	12.3	23	20.7
A7	68.39	11.3	24	24.7
A8	63.51	19.3	42	24.7
A9	88.19	4.7	28	35.3
A10	59.69	9.7	12	16

>TRINITY_DN97705_c0_g1_i1 from sample A5 / Example spliced isoform

CGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATGATTTGTGAGGGATCACCTGTGGTAGT
GTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAATGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGTGGAATCC
ATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAA
CAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATT

>TRINITY_DN97705_c0_g1_i2 from sample A5 / Example unspliced isoform:

CGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATGATTTGTGAGGGATCACCTGTGGTAGT
GTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAgtagttccaggatcttattctactctctctctcttaatttatacaggtgatataaattgtgtcatcaatg
ttataatgggaatactaggaatttattcagttaggctctggtcaataaaatcatcattggactctcctccatgggttcgacctaccttgggttttttctgaatccggggaaagggaggaaaaaactccagccacaatagcggttggtaatgtaacgggactcagccaggctagaactggagacctcgtaaaaaaacatcg
ttctacagcatgtaagtatttaacgaagatagattcctaataaaaggcctctaagtgaggtgaaagcgacgaaaaaagggtgaaaccgtctataacaaagtatctacaatgaggtttcccacagacttgcagtcgtgggccttcctcttttttactgtttccattgaggacataagttcttccgcgggcactttttaatc
aactttaaaaatgaaggtatatatatatatatatataatctcaatgtctttccgttttgttgtgttagTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATC
TTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGAC
AATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATT

Intron 3 GTATATATAT ... CTATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.73	3	12	4
A2	52.73	13	25	4
A3	75	1	5	1
A4	100	0	6	0
A5	66.67	4	11	5
A6	61.7	9	19	10
A7	54.78	26	29	34
A8	61.17	20	27	36
A9	60	19	37	20
A10	60.98	8	15	10

>TRINITY_DN46970_c2_g1_i1 from sample A7 / Example spliced isoform

TCAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATG
ATTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATAC
AGGTGATATAAATTGTGTCATCAATGTTATAATGGGAATACTAGGAATTTATTCAGTTAGGCTCTGGTCAATAAAATCATCATTGGACTCTCCTCCATGGGTTCGACCTACCTTGGGTTTTTTCTGAATCCGGGGAAAGGGAGGAAAAAACTCCAGCCACAATAGCGGTTGGTAATGTAACGGGACTCAGCCAGGCTAGA
ACTGGAGACCTCGTAAAAAAACATCGTTCTACAGCATGTAAGTATTTAACGAAGATAGATTCCTAATAAAAGGCCTCTAAGTGAGGTGAAAGCGACGAAAAAAGGGTGAAACCGTCTATAACAAAGTATCTACAATGAGGTTTCCCACAGACTTGCAGTCGTGGGCCTTCCTCTTTTTTACTGTTTCCATTGAGGACATA
AGTTCTTCCGCGGGCACTTTTTAATCAACTTTAAAAATGAAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGT
CGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATG

>TRINITY_DN46970_c2_g1_i5 from sample A7 / Example unspliced isoform:

TCAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATG
ATTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATAC
AGGTGATATAAATTGTGTCATCAATGTTATAATGGGAATACTAGGAATTTATTCAGTTAGGCTCTGGTCAATAAAATCATCATTGGACTCTCCTCCATGGGTTCGACCTACCTTGGGTTTTTTCTGAATCCGGGGAAAGGGAGGAAAAAACTCCAGCCACAATAGCGGTTGGTAATGTAACGGGACTCAGCCAGGCTAGA
ACTGGAGACCTCGTAAAAAAACATCGTTCTACAGCATGTAAGTATTTAACGAAGATAGATTCCTAATAAAAGGCCTCTAAGTGAGGTGAAAGCGACGAAAAAAGGGTGAAACCGTCTATAACAAAGTATCTACAATGAGGTTTCCCACAGACTTGCAGTCGTGGGCCTTCCTCTTTTTTACTGTTTCCATTGAGGACATA
AGTTCTTCCGCGGGCACTTTTTAATCAACTTTAAAAATGAAGgtatatatatatatatatataatctcaatgtctttccgttttgttgtgttagtgatttgaagattctaaatcaagagaagagaagagcaatgagggctattgctggagctggaggcatgaatctcattgtcattactaatgcatttaccaaaatattt
tctgcccatttcttatatgtttgttgctctgcattggtttactaatgctgctattcctctattttcagTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGT
CATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGG
AATGTCTTCGTAACTGATG

Intron 4 GTGATATAAA ... GTTGTGTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50.92	13	17	10
A2	59.66	14	26	15.5
A3	63.53	5	13	4.5
A4	66.67	4	10	6
A5	55.22	9	14	8.5
A6	64.66	9	13	20
A7	81.47	5	19	25
A8	63.8	14	26	23.5
A9	63.94	18	30	34
A10	59.44	8	8	15.5

>TRINITY_DN40809_c0_g3_i2 from sample A6 / Example spliced isoform

GTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATGATTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGA
ACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATACAGTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAG
GAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTG
GCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAA
ATTGATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

>TRINITY_DN40809_c0_g3_i6 from sample A6 / Example unspliced isoform:

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATGA
TTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATACA
Ggtgatataaattgtgtcatcaatgttataatgggaatactaggaatttattcagttaggctctggtcaataaaatcatcattggactctcctccatgggttcgacctaccttgggttttttctgaatccggggaaagggaggaaaaaactccagccacaatagcggttggtaatgtaacgggactcagccaggctagaa
ctggagacctcgtaaaaaaacatcgttctacagcatgtaagtatttaacgaagatagattcctaataaaaggcctctaagtgaggtgaaagcgacgaaaaaagggtgaaaccgtctataacaaagtatctacaatgaggtttcccacagacttgcagtcgtgggccttcctcttttttactgtttccattgaggacataa
gttcttccgcgggcactttttaatcaactttaaaaatgaaggtatatatatatatatatataatctcaatgtctttccgttttgttgtgttagTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGGCATGAATCTCATTGTCATTACTAATGCATTTACCAAAATATTTT
CTGCCCATTTCTTATATGTTTGTTGCTCTGCATTGGTTTACTAATGCTGCTATTCCTCTATTTTCAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTC
ATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGA
ATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATTGATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

Intron 5 GTAAACTGTT ... GGAGCTGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.67	2	20	6
A2	82.86	6	39	19
A3	93.33	1	23	5
A4	100	0	17	0
A5	92.59	1	14	11
A6	71.01	10	18	31
A7	56.52	25	25	40
A8	70.49	18	32	54
A9	65.22	16	26	34
A10	67.44	7	18	11

>TRINITY_DN122790_c0_g1_i3 from sample A2 / Example spliced isoform

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATT
GGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTT
CTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAG

>TRINITY_DN122790_c0_g1_i4 from sample A2 / Example unspliced isoform:

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGgtaaactgttttctttttgttctgatattgtttctctttcagcaatcccaatatgtgctgaatataatgtgttatgaatttgttttggtttgcatggatga
tttgtgagggatcacctgtggtagtgtatggatcccaaagtttcttccaaatacgcttccagcaagagttatcaatatttccatgataggatggcttgaaccaaggctacgactatgcaagtggcctccgcgatgacaaaaacatatattataaagtagttccaggatcttattctactctctctctcttaatttataca
ggtgatataaattgtgtcatcaatgttataatgggaatactaggaatttattcagttaggctctggtcaataaaatcatcattggactctcctccatgggttcgacctaccttgggttttttctgaatccggggaaagggaggaaaaaactccagccacaatagcggttggtaatgtaacgggactcagccaggctagaa
ctggagacctcgtaaaaaaacatcgttctacagcatgtaagtatttaacgaagatagattcctaataaaaggcctctaagtgaggtgaaagcgacgaaaaaagggtgaaaccgtctataacaaagtatctacaatgaggtttcccacagacttgcagtcgtgggccttcctcttttttactgtttccattgaggacataa
gttcttccgcgggcactttttaatcaactttaaaaatgaaggtatatatatatatatatataatctcaatgtctttccgttttgttgtgttagtgatttgaagattctaaatcaagagaagagaagagcaatgagggctattgctggagctggagTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAAT
ATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTC
CTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAG

Intron 6 GCATGAATCT ... CTATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.72	6.1	0	4
A2	21.51	16.6	5	4
A3	25.18	4.5	2	1
A4	-1	0	0	0
A5	30.97	9.1	3	5
A6	22.41	29.5	7	10
A7	39.97	36.2	14	34
A8	34.92	49.6	17	36
A9	26.42	32.1	3	20
A10	37.04	11.1	3	10

>TRINITY_DN40809_c0_g3_i5 from sample A6 / Example spliced isoform

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAG
GGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCT
ACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATT
GATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

>TRINITY_DN40809_c0_g3_i6 from sample A6 / Example unspliced isoform:

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATGA
TTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATACA
GGTGATATAAATTGTGTCATCAATGTTATAATGGGAATACTAGGAATTTATTCAGTTAGGCTCTGGTCAATAAAATCATCATTGGACTCTCCTCCATGGGTTCGACCTACCTTGGGTTTTTTCTGAATCCGGGGAAAGGGAGGAAAAAACTCCAGCCACAATAGCGGTTGGTAATGTAACGGGACTCAGCCAGGCTAGAA
CTGGAGACCTCGTAAAAAAACATCGTTCTACAGCATGTAAGTATTTAACGAAGATAGATTCCTAATAAAAGGCCTCTAAGTGAGGTGAAAGCGACGAAAAAAGGGTGAAACCGTCTATAACAAAGTATCTACAATGAGGTTTCCCACAGACTTGCAGTCGTGGGCCTTCCTCTTTTTTACTGTTTCCATTGAGGACATAA
GTTCTTCCGCGGGCACTTTTTAATCAACTTTAAAAATGAAGGTATATATATATATATATATAATCTCAATGTCTTTCCGTTTTGTTGTGTTAGTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGgcatgaatctcattgtcattactaatgcatttaccaaaatatttt
ctgcccatttcttatatgtttgttgctctgcattggtttactaatgctgctattcctctattttcagTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTC
ATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGA
ATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATTGATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

Intron 7 GTAAGTATTT ... GTTGTGTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.38	11.5	25	10
A2	77.64	7.3	34	15.5
A3	90.42	1.5	23	4.5
A4	64.29	5	12	6
A5	70.82	6.5	22	8.5
A6	63.73	15.5	34	20
A7	76.69	11	47	25
A8	81.68	8.8	53	23.5
A9	73.61	15.5	52	34
A10	73.77	8	29	15.5

>TRINITY_DN122790_c0_g1_i1 from sample A2 / Example spliced isoform

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATGA
TTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATACA
GGTGATATAAATTGTGTCATCAATGTTATAATGGGAATACTAGGAATTTATTCAGTTAGGCTCTGGTCAATAAAATCATCATTGGACTCTCCTCCATGGGTTCGACCTACCTTGGGTTTTTTCTGAATCCGGGGAAAGGGAGGAAAAAACTCCAGCCACAATAGCGGTTGGTAATGTAACGGGACTCAGCCAGGCTAGAA
CTGGAGACCTCGTAAAAAAACATCGTTCTACAGCATTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAG

>TRINITY_DN122790_c0_g1_i4 from sample A2 / Example unspliced isoform:

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATGA
TTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATACA
GGTGATATAAATTGTGTCATCAATGTTATAATGGGAATACTAGGAATTTATTCAGTTAGGCTCTGGTCAATAAAATCATCATTGGACTCTCCTCCATGGGTTCGACCTACCTTGGGTTTTTTCTGAATCCGGGGAAAGGGAGGAAAAAACTCCAGCCACAATAGCGGTTGGTAATGTAACGGGACTCAGCCAGGCTAGAA
CTGGAGACCTCGTAAAAAAACATCGTTCTACAGCATgtaagtatttaacgaagatagattcctaataaaaggcctctaagtgaggtgaaagcgacgaaaaaagggtgaaaccgtctataacaaagtatctacaatgaggtttcccacagacttgcagtcgtgggccttcctcttttttactgtttccattgaggacataa
gttcttccgcgggcactttttaatcaactttaaaaatgaaggtatatatatatatatatataatctcaatgtctttccgttttgttgtgttagTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAAT
ATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTC
CTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAG

Intron 8 GTAAACTGTT ... AAAAATGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	85.19	2	20	3
A2	81.25	6	39	13
A3	92.31	1	23	1
A4	100	0	17	0
A5	90	1	14	4
A6	57.45	10	18	9
A7	50.5	25	25	26
A8	59.09	18	32	20
A9	58.44	16	26	19
A10	65	7	18	8

>TRINITY_DN46970_c2_g1_i4 from sample A7 / Example spliced isoform

TCAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAAT
TGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATT
TCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATG

>TRINITY_DN46970_c2_g1_i1 from sample A7 / Example unspliced isoform:

TCAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGgtaaactgttttctttttgttctgatattgtttctctttcagcaatcccaatatgtgctgaatataatgtgttatgaatttgttttggtttgcatggatg
atttgtgagggatcacctgtggtagtgtatggatcccaaagtttcttccaaatacgcttccagcaagagttatcaatatttccatgataggatggcttgaaccaaggctacgactatgcaagtggcctccgcgatgacaaaaacatatattataaagtagttccaggatcttattctactctctctctcttaatttatac
aggtgatataaattgtgtcatcaatgttataatgggaatactaggaatttattcagttaggctctggtcaataaaatcatcattggactctcctccatgggttcgacctaccttgggttttttctgaatccggggaaagggaggaaaaaactccagccacaatagcggttggtaatgtaacgggactcagccaggctaga
actggagacctcgtaaaaaaacatcgttctacagcatgtaagtatttaacgaagatagattcctaataaaaggcctctaagtgaggtgaaagcgacgaaaaaagggtgaaaccgtctataacaaagtatctacaatgaggtttcccacagacttgcagtcgtgggccttcctcttttttactgtttccattgaggacata
agttcttccgcgggcactttttaatcaactttaaaaatgaagTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGT
CGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATG

Intron 9 GTAAACTGTT ... GTTGTGTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	88.22	2	20	10
A2	84.47	5	39	15.5
A3	87.27	2	23	4.5
A4	100	0	17	6
A5	61.29	7	14	8.5
A6	61.25	12	18	20
A7	73.52	9	25	25
A8	68.04	13	32	23.5
A9	62.63	18	26.5	34
A10	49.6	17	18	15.5

>TRINITY_DN40809_c0_g3_i5 from sample A6 / Example spliced isoform

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAG
GGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCT
ACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATT
GATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

>TRINITY_DN40809_c0_g3_i6 from sample A6 / Example unspliced isoform:

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGgtaaactgttttctttttgttctgatattgtttctctttcagcaatcccaatatgtgctgaatataatgtgttatgaatttgttttggtttgcatggatga
tttgtgagggatcacctgtggtagtgtatggatcccaaagtttcttccaaatacgcttccagcaagagttatcaatatttccatgataggatggcttgaaccaaggctacgactatgcaagtggcctccgcgatgacaaaaacatatattataaagtagttccaggatcttattctactctctctctcttaatttataca
ggtgatataaattgtgtcatcaatgttataatgggaatactaggaatttattcagttaggctctggtcaataaaatcatcattggactctcctccatgggttcgacctaccttgggttttttctgaatccggggaaagggaggaaaaaactccagccacaatagcggttggtaatgtaacgggactcagccaggctagaa
ctggagacctcgtaaaaaaacatcgttctacagcatgtaagtatttaacgaagatagattcctaataaaaggcctctaagtgaggtgaaagcgacgaaaaaagggtgaaaccgtctataacaaagtatctacaatgaggtttcccacagacttgcagtcgtgggccttcctcttttttactgtttccattgaggacataa
gttcttccgcgggcactttttaatcaactttaaaaatgaaggtatatatatatatatatataatctcaatgtctttccgttttgttgtgttagTGATTTGAAGATTCTAAATCAAGAGAAGAGAAGAGCAATGAGGGCTATTGCTGGAGCTGGAGGCATGAATCTCATTGTCATTACTAATGCATTTACCAAAATATTTT
CTGCCCATTTCTTATATGTTTGTTGCTCTGCATTGGTTTACTAATGCTGCTATTCCTCTATTTTCAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTC
ATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGA
ATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATTGATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

Intron 10 GTAAACTGTT ... CTATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	85.71	2	20	4
A2	78.18	6	39	4
A3	92.31	1	23	1
A4	100	0	17	0
A5	90.48	1	14	5
A6	58.33	10	18	10
A7	54.13	25	25	34
A8	65.38	18	32	36
A9	58.97	16	26	20
A10	66.67	7	18	10

>TRINITY_DN40809_c0_g3_i4 from sample A6 / Example spliced isoform

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATT
GGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTT
CTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATTGATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

>TRINITY_DN40809_c0_g3_i6 from sample A6 / Example unspliced isoform:

CAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGgtaaactgttttctttttgttctgatattgtttctctttcagcaatcccaatatgtgctgaatataatgtgttatgaatttgttttggtttgcatggatga
tttgtgagggatcacctgtggtagtgtatggatcccaaagtttcttccaaatacgcttccagcaagagttatcaatatttccatgataggatggcttgaaccaaggctacgactatgcaagtggcctccgcgatgacaaaaacatatattataaagtagttccaggatcttattctactctctctctcttaatttataca
ggtgatataaattgtgtcatcaatgttataatgggaatactaggaatttattcagttaggctctggtcaataaaatcatcattggactctcctccatgggttcgacctaccttgggttttttctgaatccggggaaagggaggaaaaaactccagccacaatagcggttggtaatgtaacgggactcagccaggctagaa
ctggagacctcgtaaaaaaacatcgttctacagcatgtaagtatttaacgaagatagattcctaataaaaggcctctaagtgaggtgaaagcgacgaaaaaagggtgaaaccgtctataacaaagtatctacaatgaggtttcccacagacttgcagtcgtgggccttcctcttttttactgtttccattgaggacataa
gttcttccgcgggcactttttaatcaactttaaaaatgaaggtatatatatatatatatataatctcaatgtctttccgttttgttgtgttagtgatttgaagattctaaatcaagagaagagaagagcaatgagggctattgctggagctggaggcatgaatctcattgtcattactaatgcatttaccaaaatatttt
ctgcccatttcttatatgtttgttgctctgcattggtttactaatgctgctattcctctattttcagTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTC
ATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGA
ATGTCTTCGTAACTGATGATTTCCCTTCTATGCAATATAAAATGTATTCCTTTTCAATTTTATCATGTGATTCTTTAGACACGACCAAATCTCAAAAGAAAAAATTGATATATGTTGGAATGCCTTTGTAATTGGTGGCCTTTTATAACTTTCGTTAACG

Intron 11 GTATATATAT ... GGAGCTGGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75	3	12	6
A2	62.86	13	25	19
A3	83.33	1	5	5
A4	100	0	6	0
A5	73.33	4	11	11
A6	73.53	9	19	31
A7	57.02	26	29	40
A8	66.94	20	27	54
A9	65.14	19	37	34
A10	61.9	8	15	11

>TRINITY_DN46970_c2_g1_i1 from sample A7 / Example spliced isoform

TCAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATG
ATTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATAC
AGGTGATATAAATTGTGTCATCAATGTTATAATGGGAATACTAGGAATTTATTCAGTTAGGCTCTGGTCAATAAAATCATCATTGGACTCTCCTCCATGGGTTCGACCTACCTTGGGTTTTTTCTGAATCCGGGGAAAGGGAGGAAAAAACTCCAGCCACAATAGCGGTTGGTAATGTAACGGGACTCAGCCAGGCTAGA
ACTGGAGACCTCGTAAAAAAACATCGTTCTACAGCATGTAAGTATTTAACGAAGATAGATTCCTAATAAAAGGCCTCTAAGTGAGGTGAAAGCGACGAAAAAAGGGTGAAACCGTCTATAACAAAGTATCTACAATGAGGTTTCCCACAGACTTGCAGTCGTGGGCCTTCCTCTTTTTTACTGTTTCCATTGAGGACATA
AGTTCTTCCGCGGGCACTTTTTAATCAACTTTAAAAATGAAGTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAATATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGT
CGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGTCCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATG

>TRINITY_DN46970_c2_g1_i6 from sample A7 / Example unspliced isoform:

TCAGGGGTTAGTTTTGTCCATGTATGCGGCATTATATTCATTCTCTTCTAGTTGTGTAATGGATTCTTTACGCATGCATTAGAGGAAGCCAAGAATGATGGTAAACTGTTTTCTTTTTGTTCTGATATTGTTTCTCTTTCAGCAATCCCAATATGTGCTGAATATAATGTGTTATGAATTTGTTTTGGTTTGCATGGATG
ATTTGTGAGGGATCACCTGTGGTAGTGTATGGATCCCAAAGTTTCTTCCAAATACGCTTCCAGCAAGAGTTATCAATATTTCCATGATAGGATGGCTTGAACCAAGGCTACGACTATGCAAGTGGCCTCCGCGATGACAAAAACATATATTATAAAGTAGTTCCAGGATCTTATTCTACTCTCTCTCTCTTAATTTATAC
AGGTGATATAAATTGTGTCATCAATGTTATAATGGGAATACTAGGAATTTATTCAGTTAGGCTCTGGTCAATAAAATCATCATTGGACTCTCCTCCATGGGTTCGACCTACCTTGGGTTTTTTCTGAATCCGGGGAAAGGGAGGAAAAAACTCCAGCCACAATAGCGGTTGGTAATGTAACGGGACTCAGCCAGGCTAGA
ACTGGAGACCTCGTAAAAAAACATCGTTCTACAGCATGTAAGTATTTAACGAAGATAGATTCCTAATAAAAGGCCTCTAAGTGAGGTGAAAGCGACGAAAAAAGGGTGAAACCGTCTATAACAAAGTATCTACAATGAGGTTTCCCACAGACTTGCAGTCGTGGGCCTTCCTCTTTTTTACTGTTTCCATTGAGGACATA
AGTTCTTCCGCGGGCACTTTTTAATCAACTTTAAAAATGAAGgtatatatatatatatatataatctcaatgtctttccgttttgttgtgttagtgatttgaagattctaaatcaagagaagagaagagcaatgagggctattgctggagctggagTGGAATCCATTGCAGTGGATTGCAGTGGACATGAAGGAGGGAAA
TATAATTGCGGTGGGAGAAGTAGATCTTGTGTGTATCGCAGCGAAACTAAAAAAATTGGGGTACAAAGAAGAGTTGTTGAGCCTGGGTCATGCAGAAGATCAGAAAAATGATGTCGAAGAGGAGGATCAGAAGAAATACAAGACTATAGAGCCATTGGCTCCAACAATGGTTTTGTGATGCGAAACCATGTGGCTACGGT
CCTTGTGATTAGGGATACTCGTAGACAATGATATAATCTTTGTTTTTCCTAATATTTCTTAAATTCCCAAAAAAATGATATATGTTGGAATGTCTTCGTAACTGATG