NA ----> NA

Intron 1 GTAAGACTTA ... TTTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.86	2.7	12	10
A2	100	0	8.3	4
A3	48.57	3.3	0	6
A4	100	0	5	1
A5	42.86	2	0	3
A6	66.98	5	15.7	5
A7	82.28	8	56.7	17.7
A8	77.8	11.7	51	31
A9	69.47	6	18.3	9
A10	87.62	4	35.7	21

>TRINITY_DN107870_c0_g1_i2 from sample A1 / Example spliced isoform

ATATACACAGAACAGCAAAGCTGAAATCGCCAGGAATAAGGAGATGAAATAGTGTTCAAGGACCATCGTTTGCAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCATTTTGAAGTTTGACGGCGAAAGCTTTATCTGCCAGAGATATAGATCATCTATGTTATT
TAAGCAGTATGAATTGGACTGTTCGAGGGGTGGCGGTCTCTGCAGACCAGAGAAGTTGTAATCTTTCTG

>TRINITY_DN107870_c0_g1_i1 from sample A1 / Example unspliced isoform:

ATATACACAGAACAGCAAAGCTGAAATCGCCAGGAATAAGGAGATGAAATAGTGTTCAAGGACCATCgtaagacttaaccatcgagaatttaatttaatcgctcaatcagattttcgcctcaaagatcgagaagaagagttcacctactgttttacattgtggttaaagtgttttactcgaatttatataagaaggagat
ggtattctgcatacagaaaagggggaattttctatcttttcattatttacagtttacgttgctgaaattttaataacattctaccttggtaaatgaacgttgattaatattttttttttttcagGTTTGCAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCAT
TTTGAAGTTTGACGGCGAAAGCTTTATCTGCCAGAGATATAGATCATCTATGTTATTTAAGCAGTATGAATTGGACTGTTCGAGGGGTGGCGGTCTCTGCAGACCAGAGAAGTTGTAATCTTTCTG

Intron 2 GTAAGACTTA ... TTTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80	2	12	4
A2	100	0	9	0
A3	42.86	4	0	6
A4	100	0	5	0
A5	33.33	2	0	2
A6	69.7	5	18	5
A7	82.98	8	59	19
A8	77.78	11	54	23
A9	68.42	6	19	7
A10	86.67	4	37	15

>TRINITY_DN45792_c0_g5_i9 from sample A7 / Example spliced isoform

GCATTTAAAAATGGTTGTTGAATGATGATATACACAGAACAGCAAAGCTGAAATCGCCAGGAATAAGGAGATGAAATAGTGTTCAAGGACCATCGTTTGTAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCAGTTTGAAGTTTGACGGCGAAAGCTTTATCTG
CCAGAGATATAGATCATCTATGTTATTTAAGCAGTATGAATTGGACTGTTCAGAGGGGGGTGGGCGGTCTCTGCAGACCAGAGAAATTGTAATTTTTGCTAATTCCTTTTTCTGTACATTTATGCTTGGAAAAGGGAGACTGAGGAGACGCTGCTGCCAAGGGGGAAAATCGATGGAGTTGAGGAAACTTTCGTTTCTGT
AAACTCTGTACCATTTCTTTGCTAGGGGAGCGCTCAACAGAGCTGGATAGATTTTTCTTTCAGGACTGAGGAGGCGCTGCCAAGGAGGAATCGAGGCAAGGGAATCAACATAGAAGAACTAAGGAAGCGCTGTTTCAATCCATGCATTGTAATTTTTTGC

>TRINITY_DN45792_c0_g5_i3 from sample A7 / Example unspliced isoform:

GTTAAACACCTGTGTAACAAGCGTCATGATATTAGCGTCCACGTGAAGGAGAATATGTGATTGTTATGAATTAAATTACATAAAATCTTAAGGATCGCTACCGAGAGTAAAGCTTCCACAAGAGGATAATCTTGAGTTGTTTTGCCATTTGTGCATAACTATAATCACAACATTTAAAAACGGTTGTTGAATGATGATAT
ACACAGAACAGCAAAGCTGAAATCGCCAGGAATAAGGAGATGAAATAGTGTTCAAGGACCATCgtaagacttaaccatcggggatttcatttaatcgctcaatcagattttcgcctcaaagatcgagaagaagagttcacctcctgttttacatcgtggttaaagtgttttactcgaatttatataagaaggagatggta
ttctacatacaaaaaaggggaattttctatcttttcattatttacagtttacgttgctggtaaatgaacgttgattaatatattttttttgcagGTTTGTAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCAGTTTGAAGTTTGACGGCGAAAGCTTTATCTG
CCAGAGATATAGATCATCTATGTTATTTAAGCAGTATGAATTGGACTGTTCAGAGGGGGGTGGGCGGTCTCTGCAGACCAGAGAAATTGTAATTTTTGCTAATTCCTTTTTCTGTACATTTATGCTTGGAAAAGGGAGACTGAGGAGACGCTGCTGCCAAGGGGGAAAATCGATGGAGTTGAGGAAACTTTCGTTTCTGT
AAACTCTGTACCATTTCTTTGCTAGGGGAGCGCTCAACAGAGCTGGATAGATTTTTCTTTCAGGACTGAGGAGGCGCTGCCAAGGAGGAATCGAGGCAAGGGAATCAACATAGAAGAACTAAGGAAGCGCTGTTTCAATCCATGCATTGTAATTTTTTGC

Intron 3 GTATTCTACA ... TTTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.43	12	7	4
A2	33.33	1	1	0
A3	47.37	5	3	6
A4	33.33	1	1	0
A5	42.86	2	1	2
A6	38.78	15	14	5
A7	40.19	32	24	19
A8	46.67	28	26	23
A9	37.5	10	5	7
A10	50.68	18	22	15

>TRINITY_DN45792_c0_g5_i8 from sample A7 / Example spliced isoform

GTTAAACACCTGTGTAACAAGCGTCATGATATTAGCGTCCACGTGAAGGAGAATATGTGATTGTTATGAATTAAATTACATAAAATCTTAAGGATCGCTACCGAGAGTAAAGCTTCCACAAGAGGATAATCTTGAGTTGTTTTGCCATTTGTGCATAACTATAATCACAACATTTAAAAACGGTTGTTGAATGATGATAT
ACACAGAACAGCAAAGCTGAAATCGCCAGGAATAAGGAGATGAAATAGTGTTCAAGGACCATCGTAAGACTTAACCATCGGGGATTTCATTTAATCGCTCAATCAGATTTTCGCCTCAAAGATCGAGAAGAAGAGTTCACCTCCTGTTTTACATCGTGGTTAAAGTGTTTTACTCGAATTTATATAAGAAGGAGATGGTT
TGTAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCAGTTTGAAGTTTGACGGCGAAAGCTTTATCTGCCAGAGATATAGATCATCTATGTTATTTAAGCAGTATGAATTGGACTGTTCAGAGGGGGGTGGGCGGTCTCTGCAGACCAGAGAAATTGTAATTTTT
GCTAATTCCTTTTTCTGTACATTTATGCTTGGAAAAGGGAGACTGAGGAGACGCTGCTGCCAAGGGGGAAAATCGATGGAGTTGAGGAAACTTTCGTTTCTGTAAACTCTGTACCATTTCTTTGCTAGGGGAGCGCTCAACAGAGCTGGATAGATTTTTCTTTCAGGACTGAGGAGGCGCTGCCAAGGAGGAATCGAGGC
AAGGGAATCAACATAGAAGAACTAAGGAAGCGCTGTTTCAATCCATGCATTGTAATTTTTTGC

>TRINITY_DN45792_c0_g5_i3 from sample A7 / Example unspliced isoform:

GTTAAACACCTGTGTAACAAGCGTCATGATATTAGCGTCCACGTGAAGGAGAATATGTGATTGTTATGAATTAAATTACATAAAATCTTAAGGATCGCTACCGAGAGTAAAGCTTCCACAAGAGGATAATCTTGAGTTGTTTTGCCATTTGTGCATAACTATAATCACAACATTTAAAAACGGTTGTTGAATGATGATAT
ACACAGAACAGCAAAGCTGAAATCGCCAGGAATAAGGAGATGAAATAGTGTTCAAGGACCATCGTAAGACTTAACCATCGGGGATTTCATTTAATCGCTCAATCAGATTTTCGCCTCAAAGATCGAGAAGAAGAGTTCACCTCCTGTTTTACATCGTGGTTAAAGTGTTTTACTCGAATTTATATAAGAAGGAGATGgta
ttctacatacaaaaaaggggaattttctatcttttcattatttacagtttacgttgctggtaaatgaacgttgattaatatattttttttgcagGTTTGTAGGGATGCCAAGCCGTGGAGGTTACGATATCACCGAAGATAATGTGATCGAACTGTTGTTGTGCGATCAGTTTGAAGTTTGACGGCGAAAGCTTTATCTG
CCAGAGATATAGATCATCTATGTTATTTAAGCAGTATGAATTGGACTGTTCAGAGGGGGGTGGGCGGTCTCTGCAGACCAGAGAAATTGTAATTTTTGCTAATTCCTTTTTCTGTACATTTATGCTTGGAAAAGGGAGACTGAGGAGACGCTGCTGCCAAGGGGGAAAATCGATGGAGTTGAGGAAACTTTCGTTTCTGT
AAACTCTGTACCATTTCTTTGCTAGGGGAGCGCTCAACAGAGCTGGATAGATTTTTCTTTCAGGACTGAGGAGGCGCTGCCAAGGAGGAATCGAGGCAAGGGAATCAACATAGAAGAACTAAGGAAGCGCTGTTTCAATCCATGCATTGTAATTTTTTGC