sp|P93447|EF1D_SPUBR ----> Elongation factor 1-delta

Intron 1 GTATATCATC ... ATAATGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.28	456	26	58
A2	13.63	488	19	77
A3	15.22	468	9	84
A4	13.62	501	3	79
A5	12.95	289	8	43
A6	13.54	2081	68	326
A7	13.7	1669	38	265
A8	14.79	1221	40	212
A9	14.65	1695	61	291
A10	13.24	1592	57	243

>TRINITY_DN99165_c0_g1_i4 from sample A2 / Example spliced isoform

CTTTGGATTTCTAGGGTTTAGGATTCCTGGTTTCTGAGCCTTCGAATTGTGCGAGCCTGATCATATCACTCACCCTCTGTATTTTTTCACGAGCAAAGACCATGGCGGTTATCTTCCCCAATCTGAGCACAGAGGCTGGTCTCAAGCATCTTGATGAATATCTTCTATCAAGAACGTTTATTACTGGGCATCAGGCCACA
AGAGATGATATTACAGTCTATGGCGCCATTAGCAAAGTCCCTGGACCTAACTTTCCTAATGTATCTCGCTGGTACAGCCATATCAGTGCCCTCCTGTCTTCAAGTTTTCCTGGGGAGGCTATTGGTGTAAAAATAGAAGGAGCAGCTCCAGCTGCTGCACTTGTTACTGAGACCACAAATGAGTCTCCTCCTAAAGATGA
GGAAGATGATGATGACTTGGACCTTTTTGGTGAGGAGACCGAGGAAGAAAAGAAGGCATCAGCAGAACGGGAGGCATCTAAAAAAGCATCAGGCAAAAAGAAAGAAAGTGGAAAGTCTTCTGTTCTGTTGGATGTGAAACCTTGGGATGATGAGACAGATATGAAAAAGCTGGAGGAGGTTGTGAGGAGTGTGCAAATGC
CTGGCTTGTTTTGGGGAGCTTCAAAGCTTATTGCTGTAGGATATGGTATTAAGAAGCTCCAGATTATGATGACCATTGTTGATGATCTTGTGTCTGTTGATGATCTTATCGAGGACCGCCTAACTTCTGAACCAGCAAATGAATACATTCAGAGTTGCGACATTGCTGCATTTAACAAAATCTAATGATGCAGGAAACTC
GGTTTCAAAGAGACTGCACAATGACCAACCGTGGAGGTTAATATGTATCGTATTGTTCCGATGCCTAAAAAAACCATGTTGCTGAGATGGATTCGTGTACGAGGAATGTTGTTTTTGGCTTTTAATGAGTCTTTTTAAGGGCATGCATGCCCTAATAACGGGAACTTGGTTGAAATTTATTTTTAATTGAAACGTTATAT
TCATGATCGTATTTTATATGAGAAACGTATATTCTGGCATTCAATGTGGTTTTTAGCACATGCTGTGAGTAGAAACTTGGTTGAAATTTGATTGTATTTAAGATAATATATATCTAGAGGTATTTTTTGAGGACATATTTGTGAACGCTTCACAAGTAACGAATTATGTAGCTTCGCCAACTTAGTAAAATGTTTTCGAG
GCCATGTATTCACTCTGCATATGTTTATGAA

>TRINITY_DN99165_c0_g1_i2 from sample A2 / Example unspliced isoform:

CTTTGGATTTCTAGGGTTTAGGATTCCTGGTTTCTGAGCCTTCGAATTGTGCGAGCCTGATCATATCACTCACCCTGTAAGTACCCTTGTGTTAGGTGTTTTCAGTTAGGGCCTTTTGTTAACTCTGCGTTGCCTACTTTTTCATTGAACGACTTACTGATTGAATCCAAATCTCTGTTTCACAGCTGTATTTTTTCACG
AGCAAAGACCATGGCGGTTATCTTCCCCAATCTGAGCACAGAGGCTGGTCTCAAGCATCTTGATGAATATCTTCTATCAAGAACGTTTATTACTGGgtatatcatccatctccacgatttattctgaaaataatattgaccttgcttttgtattattttgatctgctttgggactgagctcttttgttttaaattataat
ggcagGCATCAGGCCACAAGAGATGATATTACAGTCTATGGCGCCATTAGCAAAGTCCCTGGACCTAACTTTCCTAATGTATCTCGCTGGTACAGCCATATCAGTGCCCTCCTGTCTTCAAGTTTTCCTGGGGAGGCTATTGGTGTAAAAATAGAAGGAGCAGCTCCAGCTGCTGCACTTGTTACTGAGACCACAAATGA
GTCTCCTCCTAAAGATGAGGAAGATGATGATGACTTGGACCTTTTTGGTGAGGAGACCGAGGAAGAAAAGAAGGCATCAGCAGAACGGGAGGCATCTAAAAAAGCATCAGGCAAAAAGAAAGAAAGTGGAAAGTCTTCTGTTCTGTTGGATGTGAAACCTTGGGATGATGAGACAGATATGAAAAAGCTGGAGGAGGTTG
TGAGGAGTGTGCAAATGCCTGGCTTGTTTTGGGGAGCTTCAAAGCTTATTGCTGTAGGATATGGTATTAAGAAGCTCCAGATTATGATGACCATTGTTGATGATCTTGTGTCTGTTGATGATCTTATCGAGGACCGCCTAACTTCTGAACCAGCAAATGAATACATTCAGAGTTGCGACATTGCTGCATTTAACAAAATC
TAATGATGCAGGAAACTCGGTTTCAAAGAGACTGCACAATGACCAACCGTGGAGGTTAATATGTATCGTATTGTTCCGATGCCTAAAAAAACCATGTTGCTGAGATGGATTCGTGTACGAGGAATGTTGTTTTTGGCTTTTAATGAGTCTTTTTAAGGGCATGCATGCCCTAATAACGGGAACTTGGTTGAAATTTATTT
TTAATTGAAACGTTATATTCATGATCGTATTTTATATGAGAAACGTATATTCTGGCATTCAATGTGGTTTTTAGCACATGCTGTGAGTAGAAACTTGGTTGAAATTTGATTGTATTTAAGATAATATATATCTAGAGGTATTTTTTGAGGACATATTTGTGAACGCTTCACAAGTAACGAATTATGTAGCTTCGCCAACT
TAGTAAAATGTTTTCGAGGCCATGTATTCACTCTGCATATGTTTATGAA

Intron 2 GTAAGTACCC ... TGTTTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.49	458	2.8	89
A2	15.8	426.2	9.8	85.4
A3	12.16	447.2	3.4	66.8
A4	13.54	447.6	3.2	76
A5	13.61	281	0	47.4
A6	11.29	2392.2	10.2	320
A7	12.21	1906.6	6.8	283.8
A8	11.16	1348.6	8.2	180
A9	11.09	2043.4	5.4	270.2
A10	12.66	1645.6	8.6	252.4

>TRINITY_DN111396_c0_g1_i3 from sample A1 / Example spliced isoform

AAATGGTTGCTTTTTTCACCTATATATTCTCTATTTGCTATGTAGTTATTCCTCTTTTCATCTCTTTGGATTTCTAGGGTTTAGGATTCCTGGTTTCTGAGCCTTCGAATTGTGCGAGCCTGATCATATCACTCACCCTCTGTATTTTTTCACGAGCAAAGACCATGGCGGTTATCTTCCCCAATCTGAGCACAGAGGCT
GGTCTCAAGCATCTTGATGAATATCTTCTATCAAGAACGTTTATTACTGGGCATCAGGCCACAAGAGATGATATTACAGTCTATGGCGCCATTAGCAAAGTCCCTGGACCTAACTTTCCTAATGTATCTCGCTGGTACAGCCATATCAGTGCCCTCCTGTCTTCAAGTTTTCCTGGGGAGGCTATTGGTGTAAAAATAGA
AGGAGCAGCTCCAGCTGCTGCACTTGTTACTGAGACCACAAATGAGTCTCCTCCTAAAGATGAGGAAGATGATGATGACTTGGACCTTTTTGGTGAGGAGACCGAGGAAGAAAAGAAGGCATCAGCAGAACGGGAGGCATCTAAAAAAGCATCAGGCAAAAAGAAAGAAAGTGGAAAGTCTTCTGTTCTGTTGGATGTGA
AACCTTGGGATGATGAGACAGATATGAAAAAGCTGGAGGAGGTTGTGAGGAGTGTGCAAATGCCTGGCTTGTTTTGGGGAGCTTCAAAGCTTATTGCTGTAGGATATGGTATTAAGAAGCTCCAGATTATGATGACCATTGTTGATGATCTTGTGTCTGTTGATGATCTTATCGAGGACCGCCTAACTTCTGAACCAGCA
AATGAATACATTCAGAGTTGCGACATTGCTGCATTTAACAAAATCTAATGATGCAGGAAACTCGGTTTCAAAGAGACTGCACAATGACCAACCGTGGAGGTTAATATGTATCGTATTGTTCCGATGCCTAAAAAAACCATGTTGCTGAGATGGATTCGTGTACGAGGAATGTTGTTTTTGGCTTTTAATGAGTCTTTTTA
AGGGCATGCATGCCCTAATAACGGGAACTTGGTTGAAATTTATTTTTAATTGAAACGTTATATTCATGATCGTATTTTATATGAGAAACGTATATTCTGGCATTCAATGTGGTTTTTAGCACATGCTGTGAGTAGAAACTTGGTTGAAATTTGATTGTATTTAAGATAATATATATCTAGAGGTATTTTTTGAGGACATA
TTTGTGAACGCTTCACAAGTAACGAATTATGTAGCTTCGCCAACTTAGTAAAATGTTTTCGAGGCCATGTATTCACTCTGCATATGTTTATGAATCATCATATTTCTCAAAA

>TRINITY_DN111396_c0_g1_i2 from sample A1 / Example unspliced isoform:

AGCCTGATCATATCACTCACCCTgtaagtacccttgtgttaggtgttttcagttagggccttttgttaactctgcgttgcctactttttcattgaacgacttactgattgaatccaaatctctgtttcacagCTGTATTTTTTCACGAGCAAAGACCATGGCGGTTATCTTCCCCAATCTGAGCACAGAGGCTGGTCTCA
AGCATCTTGATGAATATCTTCTATCAAGAACGTTTATTACTGGGCATCAGGCCACAAGAGATGATATTACAGTCTATGGCGCCATTAGCAAAGTCCCTGGACCTAACTTTCCTAATGTATCTCGCTGGTACAGCCATATCAGTGCCCTCCTGTCTTCAAGTTTTCCTGGGGAGGCTATTGGTGTAAAAATAGAAGGAGCA
GCTCCAGCTGCTGCACTTGTTACTGAGACCACAAATGAGTCTCCTCCTAAAGATGAGGAAGATGATGATGACTTGGACCTTTTTGGTGAGGAGACCGAGGAAGAAAAGAAGGCATCAGCAGAACGGGAGGCATCTAAAAAAGCATCAGGCAAAAAGAAAGAAAGTGGAAAGTCTTCTGTTCTGTTGGATGTGAAACCTTG
GGATGATGAGACAGATATGAAAAAGCTGGAGGAGGTTGTGAGGAGTGTGCAAATGCCTGGCTTGTTTTGGGGAGCTTCAAAGCTTATTGCTGTAGGATATGGTATTAAGAAGCTCCAGATTATGATGACCATTGTTGATGATCTTGTGTCTGTTGATGATCTTATCGAGGACCGCCTAACTTCTGAACCAGCAAATGAAT
ACATTCAGAGTTGCGACATTGCTGCATTTAACAAAATCTAATGATGCAGGAAACTCGGTTTCAAAGAGACTGCACAATGACCAACCGTGGAGGTTAATATGTATCGTATTGTTCCGATGCCTAAAAAAACCATGTTGCTGAGATGGATTCGTGTACGAGGAATGTTGTTTTTGGCTTTTAATGAGTCTTTTTAAGGGCAT
GCATGCCCTAATAACGGGAACTTGGTTGAAATTTATTTTTAATTGAAACGTTATATTCATGATCGTATTTTATATGAGAAACGTATATTCTGGCATTCAATGTGGTTTTTAGCACATGCTGTGAGTAGAAACTTGGTTGAAATTTGATTGTATTTAAGATAATATATATCTAGAGGTATTTTTTGAGGACATATTTGTGA
ACGCTTCACAAGTAACGAATTATGTAGCTTCGCCAACTTAGTAAAATGTTTTCGAGGCCATGTATTCACTCTGCATATGTTTATGAATCATCATATTTCTCAAAA

Intron 3 GTATGCCTTG ... TGAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.42	510	71.7	156
A2	22.76	526	58.3	155
A3	21.14	552	39.5	148
A4	20.74	405	43.5	106
A5	22.13	278	24.8	79
A6	20.87	4456	392.6	1175
A7	21.1	3010	278.6	805
A8	20.49	2903	254.1	748
A9	20.6	3107	273.7	806
A10	20.85	2444	195.7	644

>TRINITY_DN111396_c0_g1_i2 from sample A1 / Example spliced isoform

AGCCTGATCATATCACTCACCCTGTAAGTACCCTTGTGTTAGGTGTTTTCAGTTAGGGCCTTTTGTTAACTCTGCGTTGCCTACTTTTTCATTGAACGACTTACTGATTGAATCCAAATCTCTGTTTCACAGCTGTATTTTTTCACGAGCAAAGACCATGGCGGTTATCTTCCCCAATCTGAGCACAGAGGCTGGTCTCA
AGCATCTTGATGAATATCTTCTATCAAGAACGTTTATTACTGGGCATCAGGCCACAAGAGATGATATTACAGTCTATGGCGCCATTAGCAAAGTCCCTGGACCTAACTTTCCTAATGTATCTCGCTGGTACAGCCATATCAGTGCCCTCCTGTCTTCAAGTTTTCCTGGGGAGGCTATTGGTGTAAAAATAGAAGGAGCA
GCTCCAGCTGCTGCACTTGTTACTGAGACCACAAATGAGTCTCCTCCTAAAGATGAGGAAGATGATGATGACTTGGACCTTTTTGGTGAGGAGACCGAGGAAGAAAAGAAGGCATCAGCAGAACGGGAGGCATCTAAAAAAGCATCAGGCAAAAAGAAAGAAAGTGGAAAGTCTTCTGTTCTGTTGGATGTGAAACCTTG
GGATGATGAGACAGATATGAAAAAGCTGGAGGAGGTTGTGAGGAGTGTGCAAATGCCTGGCTTGTTTTGGGGAGCTTCAAAGCTTATTGCTGTAGGATATGGTATTAAGAAGCTCCAGATTATGATGACCATTGTTGATGATCTTGTGTCTGTTGATGATCTTATCGAGGACCGCCTAACTTCTGAACCAGCAAATGAAT
ACATTCAGAGTTGCGACATTGCTGCATTTAACAAAATCTAATGATGCAGGAAACTCGGTTTCAAAGAGACTGCACAATGACCAACCGTGGAGGTTAATATGTATCGTATTGTTCCGATGCCTAAAAAAACCATGTTGCTGAGATGGATTCGTGTACGAGGAATGTTGTTTTTGGCTTTTAATGAGTCTTTTTAAGGGCAT
GCATGCCCTAATAACGGGAACTTGGTTGAAATTTATTTTTAATTGAAACGTTATATTCATGATCGTATTTTATATGAGAAACGTATATTCTGGCATTCAATGTGGTTTTTAGCACATGCTGTGAGTAGAAACTTGGTTGAAATTTGATTGTATTTAAGATAATATATATCTAGAGGTATTTTTTGAGGACATATTTGTGA
ACGCTTCACAAGTAACGAATTATGTAGCTTCGCCAACTTAGTAAAATGTTTTCGAGGCCATGTATTCACTCTGCATATGTTTATGAATCATCATATTTCTCAAAA

>TRINITY_DN111396_c0_g1_i1 from sample A1 / Example unspliced isoform:

GCCTGGCTTGTTTTGGGGAGCTTgtatgccttgtcaccctagatttattttgatcttttatctatttttattgacctttctttggtcatgagtggactttaatataatttctatgcaacggttggttctatattgcttgtctactgattctgttgggagttgaaacttacatacatgcttatgaattgcagCAAAGCTTA
TTGCTGTAGGATATGGTATTAAGAAGCTCCAGATTATGATGACCATTGTTGATGATCTTGTGTCTGTTGATGATCTTATCGAGGACCGCCTAACTTCTGAACCAGCAAATGAATACATTCAGAGTTGCGACATTGCTGCATTTAACAAAATCTAATGATGCAGGAAACTCGGTTTCAAAGAGACTGCACAATGACCAACC
GTGGAGGTTAATATGTATCGTATTGTTCCGATGCCTAAAAAAACCATGTTGCTGAGATGGATTCGTGTACGAGGAATGTTGTTTTTGGCTTTTAATGAGTCTTTTTAAGGGCATGCATGCCCTAATAACGGGAACTTGGTTGAAATTTATTTTTAATTGAAACGTTATATTCATGATCGTATTTTATATGAGAAACGTAT
ATTCTGGCATTCAATGTGGTTTTTAGCACATGCTGTGAGTAGAAACTTGGTTGAAATTTGATTGTATTTAAGATAATATATATCTAGAGGTATTTTTTGAGGACATATTTGTGAACGCTTCACAAGTAACGAATTATGTAGCTTCGCCAACTTAGTAAAATGTTTTCGAGGCCATGTATTCACTCTGCATATGTTTATGA
ATCATCATATTTCTCAAAA

Intron 4 GTGAGCATCT ... ACATTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	7.02	640.9	49.5	13
A2	9.8	536.5	60.3	18.9
A3	8.31	465.8	43.5	21.4
A4	10.07	503.3	58.4	26.1
A5	14.27	257.5	45	9.4
A6	9.09	3334.9	344.6	93.8
A7	9.61	2254.9	248	79.4
A8	9.13	2248	233.3	73.5
A9	9.47	2435.9	263.6	84.3
A10	9.64	1875	207.3	76.5

>TRINITY_DN92050_c1_g1_i1 from sample A5 / Example spliced isoform

AAATGGTTGCTTTTTTCACCTATATATTCTCTATTTGCTATGTAGTTATTCCTCTTTTCATCTCTTTGGATTTCTAGGGTTTAGGATTCCTGGTTTCTGAGCCTTCGAATTGTGCGAGCCTGATCATATCACTCACCCTCTGTATTTTTTCACGAGCAAAGACCATGGCGGTTATCTTCCCCAATCTGAGCACAGAGGCT
GGTCTCAAGCATCTTGATGAATATCTTCTATCAAGAACGTTTATTACTGGGCATCAGGCCACAAGAGATGATATTACAGTCTATGGCGCCATTAGCAAAGTCCCTGGACCTAACTTTCCTAATGTATCTCGCTGGTACAGCCATATCAGTGCCCTCCTGTCTTCAAGTTTTCCTGGGGAGGCTATTGGTGTAAAAATAGA
AGGAGCAGCTCCAGCTGCTGCACTTGTTACTGAGACCACAAATGAGTCTCCTCCTAAAGATGAGGAAGATGATGATGACTTGGACCTTTTTGGTGAGGAGACCGAGGAAGAAAAGAAGGCATCAGCAGAACGGGAGGCATCTAAAAAAGCATCAGGCAAAAAGAAAGAAAGTGGAAAGTCTTCTGTTCTGTTGGATGTGA
AACCTTGGGATGATGAGACAGATATGAAAAAGCTGGAGGAGGTTGTGAGGAGTGTGCAAATGCCTGGCTTGTTTTGGGGAGCTTCAAAGCTTATTGCTGTAGGATATGGTATTAAGAAGCTCCAGATTATGATGACCATTGTTGATGATCTTGTGTCTGTTGATGATCTTATCGAGGACCGCCTAACTTCTGAACCAGCA
AATGAATACATTCAGAGTTGCGACATTGCTGCATTTAACAAAATCTAATGATGCAGGAAACTCGGTTTCAAAGAGACTGCACAATGACCAACCGTGGAGGTTAATATGTATCGTATTGTTCCGATGCCTAAAAAAACCATGTTGCTGAGATGGATTCGTGTACGAGGAATGTTGTTTTTGGCTTTTAATGAGTCTTTTTA
AGGGCATGCATGCCCTAATAACGGGAACTTGGTTGAAATTTATTTTTAATTGAAACGTTATATTCATGATCGTATTTTATATGAGAAACGTATATTCTGGCATTCAATGTGGTTTTTAGCACATGCTGTGAGTAGAAACTTGGTTGAAATTTGATTGTATTTAAGATAATATATATCTAGAGGTATTTTTTGAGGACATA
TTTGTGAACGCTTCACAAGTAACGAATTATGTAGCTTCGCCAACTTAGTAAAATGTTTTCGAGGCCATGTATTCACTCTGCATATGTTTATGAATCATCATATTTCTCAGTTCTTTGGTACATGCC

>TRINITY_DN92050_c1_g1_i2 from sample A5 / Example unspliced isoform:

AAATGGTTGCTTTTTTCACCTATATATTCTCTATTTGCTATGTAGTTATTCCTCTTTTCATCTCTTTGGATTTCTAGGGTTTAGGATTCCTGGTTTCTGAGCCTTCGAATTGTGCGAGCCTGATCATATCACTCACCCTCTGTATTTTTTCACGAGCAAAGACCATGGCGGTTATCTTCCCCAATCTGAGCACAGAGGCT
GGTCTCAAGCATCTTGATGAATATCTTCTATCAAGAACGTTTATTACTGGGCATCAGGCCACAAGAGATGATATTACAGTCTATGGCGCCATTAGCAAAGTCCCTGGACCTAACTTTCCTAATGTATCTCGCTGGTACAGCCATATCAGTGCCCTCCTGTCTTCAAGTTTTCCTGGGGAGGCTATTGGTGTAAAAATAGA
AGGAGCAGCTCCAGCTGCTGCACTTGTTACTGAGACCACAAATGAGTCTCCTCCTAAAGATGAGGAAGATGATGATGACTTGGACCTTTTTGGTGAGGAGACCGAGGAAGAAAAGAAGGCATCAGCAGAACGGGAGGCATCTAAAAAAGCATCAGGCAAAAAGAAAGAAAgtgagcatctatttgtttgcctcgatctcg
tttcttgaaaattatgtgccttcaggtcccatctattgacgtggttgtatctgtttaaaacattaacagGTGGAAAGTCTTCTGTTCTGTTGGATGTGAAACCTTGGGATGATGAGACAGATATGAAAAAGCTGGAGGAGGTTGTGAGGAGTGTGCAAATGCCTGGCTTGTTTTGGGGAGCTTGTATGCCTTGTCACCCT
AGATTTATTTTGATCTTTTATCTATTTTTATTGACCTTTCTTTGGTCATGAGTGGACTTTAATATAATTTCTATGCAACGGTTGGTTCTATATTGCTTGTCTACTGATTCTGTTGGGAGTTGAAACTTACATACATGCTTATGAATTGCAGCAAAGCTTATTGCTGTAGGATATGGTATTAAGAAGCTCCAGATTATGAT
GACCATTGTTGATGATCTTGTGTCTGTTGATGATCTTATCGAGGACCGCCTAACTTCTGAACCAGCAAATGAATACATTCAGAGTTGCGACATTGCTGCATTTAACAAAATCTAATGATGCAGGAAACTCGGTTTCAAAGAGACTGCACAATGACCAACCGTGGAGGTTAATATGTATCGTATTGTTCCGATGCCTAAAA
AAACCATGTTGCTGAGATGGATTCGTGTACGAGGAATGTTGTTTTTGGCTTTTAATGAGTCTTTTTAAGGGCATGCATGCCCTAATAACGGGAACTTGGTTGAAATTTATTTTTAATTGAAACGTTATATTCATGATCGTATTTTATATGAGAAACGTATATTCTGGCATTCAATGTGGTTTTTAGCACATGCTGTGAGT
AGAAACTTGGTTGAAATTTGATTGTATTTAAGATAATATATATCTAGAGGTATTTTTTGAGGACATATTTGTGAACGCTTCACAAGTAACGAATTATGTAGCTTCGCCAACTTAGTAAAATGTTTTCGAGGCCATGTATTCACTCTGCATATGTTTATGAATCATCATATTTCTCAGTTCTTTGGTACATGCC