sp|Q6NQ66|GGLO2_ARATH ----> L-gulonolactone oxidase 2

Intron 1 GTTTGTAACT ... CTGTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.75	9	1	7
A2	18.18	9	1.5	2
A3	0	1	0.5	0
A4	0	1	0.5	0
A5	85.71	1	3.5	6
A6	12.5	21	6	3
A7	11.11	32	10.5	4
A8	23.33	23	3.5	7
A9	13.33	26	8.5	4
A10	15.22	39	13	7

>TRINITY_DN37395_c0_g1_i2 from sample A6 / Example spliced isoform

CAAGAAAACTACGACGTTGGTTTCTGAGTGCAGGTGACGCTGAAGCTGCAACCCATGTTTAAGCGCTCCATAACAAACATAAAGATGAACGACACGTATTTAGAGGACAATATAGTTGACTTTGGAATGGCACATGAATTCGCAGACTTCACATGGTTTCCTGGGCAGCGCGAGGTAATCTACAGAATGGACGATCGCGT
TTCAGTTGATGAACCAGGAAACGGGATCAACGACTTCATCGGCTTTCGCCCCGCTTTGACAGCAGGCCTAGCCATAGCTCGTTCAACGGGTACGTTATAAAAGAACCCAACGGTCAAAAGTTACAGCAGAGGAGCTCCAAGAAGATACAAAGGATGCCAAGGGGAAGTGCATCACAGCCAAGGTTCAAGTTGGGGTCATT
TTAAGTCTGGCTTCAGGTTTTAAAAACAACGGTCTATTCTTCACAAAGTATCCTGTGATAGGTTATCAGAACAATCTGCAGGCCTCTGGATCATGTCTGAAATCTGCAGAAGATGTCTTGCTCACAGCTTGTGCATGGGATCCTCGCATCAAAGGCGAATTCTTCCATCAGACAACCATAGCCATTGGTCTTTCCAAGAT
CAAAGATTTCATTGCCGACATTAAGAAGCTTCGGGACCTGAGCCCCAGCAGTCTGTGTGGAATCGAGCTCTACAGTGGCATTCTGATGCGTTTTGTGAAAGCTTCCACTGCATATTTAGGTTTAGACGAGGATTCTGTGGACCTGGATATCACTTATTACCGTTCTCGAGATCCCATGACTCCCAGGCTGAACGAAGATG
TGTTGGAAGAGATTGAGCAGATGGGTTTGTTCAAATATGGAGGTACTCCTCACTGGGGCAAGAACCGAAATATTGCATTTCTGGGAGTCATGGACAAGTTCAGTAAAGCCCGCGATTTCTTGAAAGCAAATACAAAATACGATCCTGCAGGATTGATGTCGAATGAGTGGACAGATGCGATGATGGGGGCAGGGCAGGAA
GGGTTGGTTATAGTGAAGGATGGATGTGCTTTGGAAGGGCTGTGTGTGTGCTCTGAGGACAGACACTGTGCTCCTCACAAGGGTTATTTCTGTAGAGCAGGAAGAGTCTACCCAGATGCTAGGGTTTGTAGATATGAAGGATAGGAAAGCATGCCCATGAATCATTTGCATAACAATGGAAATGTAAATGGCAAATTAAT
CTAAGGTTTTCAATTCTCAGTTTAGTTTCATGAATTTGTTTATTTTTACATGACAGTTGTTTA

>TRINITY_DN37395_c0_g1_i3 from sample A6 / Example unspliced isoform:

CAACGGTCAAAAGTTACAGCAGgtttgtaacttcaagaatttgttcaagaaaattccaagttagaatgaatctgagctcttatttccatgtatttatgatactatggattttggctgtcttcagAGGAGCTCCAAGAAGATACAAAGGATGCCAAGGGGAAGTGCATCACAGCCAAGGTTCAAGTTGGGGTCATTTTAAG
TCTGGCTTCAGGTTTTAAAAACAACGGTCTATTCTTCACAAAGTATCCTGTGATAGGTTATCAGAACAATCTGCAGGCCTCTGGATCATGTCTGAAATCTGCAGAAGATGTCTTGCTCACAGCTTGTGCATGGGATCCTCGCATCAAAGGCGAATTCTTCCATCAGACAACCATAGCCATTGGTCTTTCCAAGATCAAAG
ATTTCATTGCCGACATTAAGAAGCTTCGGGACCTGAGCCCCAGCAGTCTGTGTGGAATCGAGCTCTACAGTGGCATTCTGATGCGTTTTGTGAAAGCTTCCACTGCATATTTAGGTTTAGACGAGGATTCTGTGGACCTGGATATCACTTATTACCGTTCTCGAGATCCCATGACTCCCAGGCTGAACGAAGATGTGTTG
GAAGAGATTGAGCAGATGGGTTTGTTCAAATATGGAGGTACTCCTCACTGGGGCAAGAACCGAAATATTGCATTTCTGGGAGTCATGGACAAGTTCAGTAAAGCCCGCGATTTCTTGAAAGCAAATACAAAATACGATCCTGCAGGATTGATGTCGAATGAGTGGACAGATGCGATGATGGGGGCAGGGCAGGAAGGGTT
GGTTATAGTGAAGGATGGATGTGCTTTGGAAGGGCTGTGTGTGTGCTCTGAGGACAGACACTGTGCTCCTCACAAGGGTTATTTCTGTAGAGCAGGAAGAGTCTACCCAGATGCTAGGGTTTGTAGATATGAAGGATAGGAAAGCATGCCCATGAATCATTTGCATAACAATGGAAATGTAAATGGCAAATTAATCTAAG
GTTTTCAATTCTCAGTTTAGTTTCATGAATTTGTTTATTTTTACATGACAGTTGTTTA

Intron 2 GTACGTTATA ... GTTACAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	13	4	9
A2	48.15	7	4	9
A3	28	9	6	1
A4	33.33	3	2	1
A5	50	1	1	1
A6	65.79	13	29	21
A7	61.6	24	45	32
A8	56.96	17	22	23
A9	56.52	25	39	26
A10	58.94	31	50	39

>TRINITY_DN37395_c0_g1_i1 from sample A6 / Example spliced isoform

CAAGAAAACTACGACGTTGGTTTCTGAGTGCAGGTGACGCTGAAGCTGCAACCCATGTTTAAGCGCTCCATAACAAACATAAAGATGAACGACACGTATTTAGAGGACAATATAGTTGACTTTGGAATGGCACATGAATTCGCAGACTTCACATGGTTTCCTGGGCAGCGCGAGGTAATCTACAGAATGGACGATCGCGT
TTCAGTTGATGAACCAGGAAACGGGATCAACGACTTCATCGGCTTTCGCCCCGCTTTGACAGCAGGCCTAGCCATAGCTCGTTCAACGGAGGAGCTCCAAGAAGATACAAAGGATGCCAAGGGGAAGTGCATCACAGCCAAGGTTCAAGTTGGGGTCATTTTAAGTCTGGCTTCAGGTTTTAAAAACAACGGTCTATTCT
TCACAAAGTATCCTGTGATAGGTTATCAGAACAATCTGCAGGCCTCTGGATCATGTCTGAAATCTGCAGAAGATGTCTTGCTCACAGCTTGTGCATGGGATCCTCGCATCAAAGGCGAATTCTTCCATCAGACAACCATAGCCATTGGTCTTTCCAAGATCAAAGATTTCATTGCCGACATTAAGAAGCTTCGGGACCTG
AGCCCCAGCAGTCTGTGTGGAATCGAGCTCTACAGTGGCATTCTGATGCGTTTTGTGAAAGCTTCCACTGCATATTTAGGTTTAGACGAGGATTCTGTGGACCTGGATATCACTTATTACCGTTCTCGAGATCCCATGACTCCCAGGCTGAACGAAGATGTGTTGGAAGAGATTGAGCAGATGGGTTTGTTCAAATATGG
AGGTACTCCTCACTGGGGCAAGAACCGAAATATTGCATTTCTGGGAGTCATGGACAAGTTCAGTAAAGCCCGCGATTTCTTGAAAGCAAATACAAAATACGATCCTGCAGGATTGATGTCGAATGAGTGGACAGATGCGATGATGGGGGCAGGGCAGGAAGGGTTGGTTATAGTGAAGGATGGATGTGCTTTGGAAGGGC
TGTGTGTGTGCTCTGAGGACAGACACTGTGCTCCTCACAAGGGTTATTTCTGTAGAGCAGGAAGAGTCTACCCAGATGCTAGGGTTTGTAGATATGAAGGATAGGAAAGCATGCCCATGAATCATTTGCATAACAATGGAAATGTAAATGGCAAATTAATCTAAGGTTTTCAATTCTCAGTTTAGTTTCATGAATTTGTT
TATTTTTACATGACAGTTGTTTA

>TRINITY_DN37395_c0_g1_i4 from sample A6 / Example unspliced isoform:

CAAGAAAACTACGACGTTGGTTTCTGAGTGCAGGTGACGCTGAAGCTGCAACCCATGTTTAAGCGCTCCATAACAAACATAAAGATGAACGACACGTATTTAGAGGACAATATAGTTGACTTTGGAATGGCACATGAATTCGCAGACTTCACATGGTTTCCTGGGCAGCGCGAGGTAATCTACAGAATGGACGATCGCGT
TTCAGTTGATGAACCAGGAAACGGGATCAACGACTTCATCGGCTTTCGCCCCGCTTTGACAGCAGGCCTAGCCATAGCTCGTTCAACGGgtacgttataaaagaacccaacggtcaaaagttacagcagAGGAGCTCCAAGAAGATACAAAGGATGCCAAGGGGAAGTGCATCACAGCCAAGGTTCAAGTTGGGGTCATT
TTAAGTCTGGCTTCAGGTTTTAAAAACAACGGTCTATTCTTCACAAAGTATCCTGTGATAGGTTATCAGAACAATCTGCAGGCCTCTGGATCATGTCTGAAATCTGCAGAAGATGTCTTGCTCACAGCTTGTGCATGGGATCCTCGCATCAAAGGCGAATTCTTCCATCAGACAACCATAGCCATTGGTCTTTCCAAGAT
CAAAGATTTCATTGCCGACATTAAGAAGCTTCGGGACCTGAGCCCCAGCAGTCTGTGTGGAATCGAGCTCTACAGTGGCATTCTGATGCGTTTTGTGAAAGCTTCCACTGCATATTTAGGTTTAGACGAGGATTCTGTGGACCTGGATATCACTTATTACCGTTCTCGAGATCCCATGACTCCCAGGCTGAACGAAGATG
TGTTGGAAGAGATTGAGCAGATGGGTTTGTTCAAATATGGAGGTACTCCTCACTGGGGCAAGAACCGAAATATTGCATTTCTGGGAGTCATGGACAAGTTCAGTAAAGCCCGCGATTTCTTGAAAGCAAATACAAAATACGATCCTGCAGGATTGATGTCGAATGAGTGGACAGATGCGATGATGGGGGCAGGGCAGGAA
GGGTTGGTTATAGTGAAGGATGGATGTGCTTTGGAAGGGCTGTGTGTGTGCTCTGAGGACAGACACTGTGCTCCTCACAAGGGTTATTTCTGTAGAGCAGGAAGAGTCTACCCAGATGCTAGGGTTTGTAGATATGAAGAATAGGAAAATATACTCATCAATCGTTTGCATAACCATGACACACAAATGGTTATCAATCG
TTATCAAAGTGACTCCTCATTGTCAATTGTCCGTTGATAGAAAAACTACAATATTTGAAAATTACAGTAGTCAACACAAAAATTACAAAAGTAAACACATTGTATATATGGTTTCTTATCACTAACCAAAAATACTTCCATTGGTTTGACTAATATTGAAACTATTACACATGTACATCCTAACTCTGGTTGGAGCCTTG
GAGGC

Intron 3 GTACGTTATA ... CTGTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.53	13	4	7
A2	36.36	7	4	2
A3	40	9	6	0
A4	40	3	2	0
A5	50	1	1	6
A6	59.38	13	19	3
A7	61.29	24	38	4
A8	41.38	17	12	7
A9	56.9	25	33	4
A10	51.56	31	33	7

>TRINITY_DN38438_c0_g1_i2 from sample A7 / Example spliced isoform

GGCGGACGACGTTGGTTTCTGAGTGCAGGTGACGCTGAAGCTGCAACCCATGTTTAAGCGCTCCATAACAAACATAAAGATGAACGACACGTATTTAGAGGACAATATAGTTGACTTTGGAATGGCACATGAATTCGCAGACTTCACATGGTTTCCTGGGCAGCGCGAGGTAATCTACAGAATGGACGATCGCGTTTCAG
TTGATGAACCAGGAAACGGGATCAACGACTTCATCGGCTTTCGCCCCGCTTTGACAGCAGGCCTAGCCATAGCTCGTTCAACGGAGGAGCTCCAAGAAGATACAAAGGATGCCAAGGGGAAGTGCATCACAGCCAAGGTTCAAGTTGGGGTCATTTTAAGTCTGGCTTCAGGTTTTAAAAACAACGGTCTATTCTTCACA
AAGTATCCTGTGATAGGTTATCAGAACAATCTGCAGGCCTCTGGATCATGTCTGAAATCTGCAGAAGATGTCTTGCTCACAGCTTGTGCATGGGATCCTCGCATCAAAGGCGAATTCTTCCATCAGACAACCATAGCCATTGGTCTTTCCAAGATCAAAGATTTCATTGCCGACATTAAGAAGCTTCGGGACCTGAGCCC
CAGCAGTCTGTGTGGAATCGAGCTCTACAGTGGCATTCTGATGCGTTTTGTGAAAGCTTCCACTGCATATTTAGGTTTAGACGAGGATTCTGTGGACCTGGATATCACTTATTACCGTTCTCGAGATCCCATGACTCCCAGGCTGAACGAAGATGTGTTGGAAGAGATTGAGCAGATGGGTTTGTTCAAATATGGAGGTA
CTCCTCACTGGGGCAAGAACCGAAATATTGCATTTCTGGGAGTCATGGACAAGTTCAGTAAAGCCCGCGATTTCTTGAAAGCAAATACAAAATACGATCCTGCAGGATTGATGTCGAATGAGTGGACAGATGCGATGATGGGGGCAGGGCAGGAAGGGTTGGTTATAGTGAAGGATGGATGTGCTTTGGAAGGGCTGTGT
GTGTGCTCTGAGGACAGACACTGTGCTCCTCACAAGGGTTATTTCTGTAGAGCAGGAAGAGTCTACCCAGATGCTAGGGTTTGTAGATATGAAGGATAGGAAAGCATGCCCATGAATCATTTGCATAACAATGGAAATGTAAATGGCAAATTAATCTAAGGTTTTCAATTCTCAGTTTAGTTTCATGA

>TRINITY_DN38438_c0_g1_i3 from sample A7 / Example unspliced isoform:

GGCGGACGACGTTGGTTTCTGAGTGCAGGTGACGCTGAAGCTGCAACCCATGTTTAAGCGCTCCATAACAAACATAAAGATGAACGACACGTATTTAGAGGACAATATAGTTGACTTTGGAATGGCACATGAATTCGCAGACTTCACATGGTTTCCTGGGCAGCGCGAGGTAATCTACAGAATGGACGATCGCGTTTCAG
TTGATGAACCAGGAAACGGGATCAACGACTTCATCGGCTTTCGCCCCGCTTTGACAGCAGGCCTAGCCATAGCTCGTTCAACGGgtacgttataaaagaacccaacggtcaaaagttacagcaggtttgtaacttcaagaatttgttcaagaaaattccaagttagaatgaatctgagctcttatttccatgtatttatg
atactatggattttggctgtcttcagAGGAGCTCCAAGAAGATACAAAGGATGCCAAGGGGAAGTGCATCACAGCCAAGGTTCAAGTTGGGGTCATTTTAAGTCTGGCTTCAGGTTTTAAAAACAACGGTCTATTCTTCACAAAGTATCCTGTGATAGGTTATCAGAACAATCTGCAGGCCTCTGGATCATGTCTGAAAT
CTGCAGAAGATGTCTTGCTCACAGCTTGTGCATGGGATCCTCGCATCAAAGGCGAATTCTTCCATCAGACAACCATAGCCATTGGTCTTTCCAAGATCAAAGATTTCATTGCCGACATTAAGAAGCTTCGGGACCTGAGCCCCAGCAGTCTGTGTGGAATCGAGCTCTACAGTGGCATTCTGATGCGTTTTGTGAAAGCT
TCCACTGCATATTTAGGTTTAGACGAGGATTCTGTGGACCTGGATATCACTTATTACCGTTCTCGAGATCCCATGACTCCCAGGCTGAACGAAGATGTGTTGGAAGAGATTGAGCAGATGGGTTTGTTCAAATATGGAGGTACTCCTCACTGGGGCAAGAACCGAAATATTGCATTTCTGGGAGTCATGGACAAGTTCAG
TAAAGCCCGCGATTTCTTGAAAGCAAATACAAAATACGATCCTGCAGGATTGATGTCGAATGAGTGGACAGATGCGATGATGGGGGCAGGGCAGGAAGGGTTGGTTATAGTGAAGGATGGATGTGCTTTGGAAGGGCTGTGTGTGTGCTCTGAGGACAGACACTGTGCTCCTCACAAGGGTTATTTCTGTAGAGCAGGAA
GAGTCTACCCAGATGCTAGGGTTTGTAGATATGAAGGATAGGAAAGCATGCCCATGAATCATTTGCATAACAATGGAAATGTAAATGGCAAATTAATCTAAGGTTTTCAATTCTCAGTTTAGTTTCATGA