sp|Q8RWW0|ALE2_ARATH ----> Receptor-like serine/threonine-protein kinase ALE2

Intron 1 GTTGTTCTGA ... CTTGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	7	8
A2	75.76	4	10	15
A3	88.24	1	8	7
A4	100	0	13	8
A5	90	1	9	9
A6	86.75	10	73	58
A7	88.79	6	45	50
A8	82.2	17	72	85
A9	89.55	7	57	63
A10	94.52	4	67	71

>TRINITY_DN70585_c0_g1_i1 from sample A2 / Example spliced isoform

GCAGATCTGACCAGCATCCGAGGAAATCTAGGGTTGGCGATATGGGGCCTTCAAATAATATCTAGTGTTTGCAAGCCAATGGTTTGGTCTGCCTCTGATATGCATTAAAGCAATATTCTTCTGTGTTCGAAGCATGACATCTGCAGTGTTTGGAATATGGTATGTGGTGGTGGGAGCTTGAAGCCACCTAAAACTCGCAG
ATCTGACCAGCATCCGAGGAAATCTAGGGTTGGCGATATGGGGCCTTCAAATAATATCTAGTGTTTGCAAGCCAATGGTTTGGTCTGCCTCTGATATGCATTAAAGCAATATTCTTCTGTGTTCGAAGCATGACATCTGCAGTGTCAATATAGGGTTGGCCATATGCTTCCTTCAAATAATATCTAGGGTTCGCAAGCCA
ATGGTCGGCCGCATAATCTGTTGTTGGCCTGATGAATTGAACTGGAAAATCCATTTTCGGTCCTCAAATTAAGGGAAGTTGTGGAATATACGAATTTGCAAAAAGGGCCCAATGTGCGCAACCGCCCATACAAATATTTTTGCAGTCGCTCAACCTGTGATTTTACATTCACTGTAAGTCCCGGTGGAGGAGTCATGTAT
ATCTCCTTATGCATATCACTTTCAAGTAACGCATTATAAACGTCCATTTGATACAGTTTCCTGTGGAAAGACAGAGCTAGAATGGGAAGAGAAGATGTCGATGGAAGAGGCCCAACACCTGAAGGCATGACGATGGTACCCATGATACTTCCATATGGG

>TRINITY_DN70585_c0_g1_i3 from sample A2 / Example unspliced isoform:

GCAGATCTGACCAGCATCCGAGGAAATCTAGGGTTGGCGATATGGGGCCTTCAAATAATATCTAGTGTTTGCAAGCCAATGGTTTGGTCTGCCTCTGATATGCATTAAAGCAATATTCTTCTGTGTTCGAAGCATGACATCTGCAGTGTTTGGAATATGGTATGTGGTGGTGGGAGCTTGAAGCCACCTAAAACTCGCAG
ATCTGACCAGCATCCGAGGAAATCTAGGGTTGGCGATATGGGGCCTTCAAATAATATCTAGTGTTTGCAAGCCAATGGTTTGGTCTGCCTCTGATATGCATTAAAGCAATATTCTTCTGTGTTCGAAGCATGACATCTGCAGTGTCAATATAGGGTTGGCCATATGCTTCCTTCAAATAATATCTAGGGTTCGCAAGCCA
ATGGTCGGCCGCATAATCTGTTGTTGGCCTGgttgttctgatttccttcaaacccacaaatttcttgctctatttttcctttcggactttcagagattcaatccatcggacttgatgcagATGAATTGAACTGGAAAATCCATTTTCGGTCCTCAAATTAAGGGAAGTTGTGGAATATACGAATTTGCAAAAAGGGCCCA
ATGTGCGCAACCGCCCATACAAATATTTTTGCAGTCGCTCAACCTGTGATTTTACATTCACTGTAAGTCCCGGTGGAGGAGTCATGTATATCTCCTTATGCATATCACTTTCAAGTAACGCATTATAAACGTCCATTTGATACAGTTTCCTGTGGAAAGACAGAGCTAGAATGGGAAGAGAAGATGTCGATGGAAGAGGC
CCAACACCTGAAGGCATGACGATGGTACCCATGATACTTCCATATGGG

Intron 2 GTAATCCCCC ... AATGCATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.86	4	5	1
A2	33.33	4	4	0
A3	50	2	2	2
A4	100	0	1	1
A5	73.33	2	6	5
A6	51.02	12	22	3
A7	41.82	16	12	11
A8	40	9	9	3
A9	33.33	11	11	0
A10	37.04	17	15	5

>TRINITY_DN46132_c0_g3_i11 from sample A10 / Example spliced isoform

GTCTACAGATCCAGTGGTTGACCAAGACAAGCTTGCAGAAGCAAAGGTGCCTAGACCAGTTCGAGATTGAGATCCATGGAGGGTCTACAGATCCAGTGGTTGACCGAGATTGGATAAGAAAAGGGGCCTAGAGAAGTTCAAGATCGAGATCTAGGGAGGGTTTACAGATCCAGTGGTTTATCCAAACGAGCCTACAGAAG
CAAAGGTGACTGGAGAAGTTTGATATCGAGATTCGGGGGAGGGTTTATAGATCTAGTGGTTTACCGAGACGAGCTTGCAGAAGCAAAGATGCCTGGAACAGTTCTAGATCAAGATCCTTAGCCGTCAACCATAGAGGCACAAGGAGTAGAGGCGGCATTCGAGCTAGATCAAGAGCCATAGCTTGTCAAAAGTCCTGTTA
TTTCTTTCTTTATCATGTGTTTCTTTTGTGACAGAAAAATGAGAGATAATCACAGGACGCCTATAGGTTCAGGGTCAAACTTCGACTTATTATCGCCGGAATTGCAGATATTTTTCACCACCATGGCCAAGCATAGACTGAAGGTTAGTGCCAGGAAGCCAAGAGCCAATCACTTAACTTAGGATTCATTGCTGTCGATC
ATAGTCTGTATAATTTCCAGGAGGTAAGGTGCCAGAAGATAAGGTGATCGCCGCCGTCCTGTTGTCATTGCAAGTTCGCTTAGATCTACTGGTTTTGATTAAGGAGAATACGAGATTCATGAATGGTTAGATTTTGGTTTAAATTATTCATGTTGTTACACAGTAATTAGATCCATCAGTTAAAAGAAATTTGTTGTTCC

>TRINITY_DN46132_c0_g3_i2 from sample A10 / Example unspliced isoform:

GTCTACAGATCCAGTGGTTGACCAAGACAAGCTTGCAGAAGCAAAGGTGCCTAGACCAGTTCGAGATTGAGATCCATGGAGGGTCTACAGATCCAGTGGTTGACCGAGATTGGATAAGAAAAGGGGCCTAGAGAAGTTCAAGATCGAGATCTAGGGAGGGTTTACAGATCCAGTGGTTTATCCAAACGAGCCTACAGAAG
CAAAGGTGACTGGAGAAGTTTGATATCGAGATTCGGGGGAGGGTTTATAGATCTAGTGGTTTACCGAGACGAGCTTGCAGAAGCAAAGATGCCTGGAACAGTTCTAGATCAAGATCCTTAGCCGTCAACCATAGAGGCACAAGGAGTAGAGGCAGCATTCGAGCTAGATCGACAGCTATAGCTTGTCAAAAGTCCTGTTA
TTTCTTTCTTTATCATGTGTTTCTTTTGTGACAGAAAAATGAGAGATAATCACAGGACGCCTATAGGTTCAGGGTCAAACTTCGACTTATTATCGCCGgtaatcccccttcctcaaatcatgaatgctgttaccatgcaaattacttaaatatgctcttgcctgatttggtatctctagttctcagcgcttatttgggct
ctgcctttatttttatcatttgggttttggctgtgcaaatcgttttatgttgcttgcgggttaccaatatttttttaagcttatgtgattaaaagggctaagttggtttcagagatatcttatggattagtgattgcattatttttatcatttgggttttggctgtgcaaaccgttttctgttgcttgcgggttgcaaat
atttttttaagcttatgtgattaaaagggctaagttagtttcagagatatcttctggattagtgattgcattatttttatcatttgggttttggctgtgcaaatcgttttctgttgcttgcgggttgccaatatttttttaagcctatgtgattaaaagggctaagttggtttcagagatatcttctggattggtgattg
caaatagagttttttttcttagattcttgtgagggttctggatccatgataatataatttcaatttgcttttttatttgtgaatatggtattcgcttaagacatccaaaaagatggaggataatatggaaagatggaaaataatatgaaattattatagtattcattgtttgattcccattcatcatgaatcaatgcata
agGAATTGCAGATATTTTTCACCACCATGGCCAAGCATAGACTGAAGGTTAGTGCCAGGAAGCCAAGAGCCAATCACTTAACTTAGGATTCATTGCTGTCGATCATAGTCTGTATAATTTCCAGGAGGTAAGGTGCCAGAAGATAAGGTGATCGCCGCCGTCCTGTTGTCATTGCAAGTTCGCTTAGATCTACTGGTTTT
GATTAAGGAGAATACGAGATTCATGAATGGTTAGATTTTGGTTTAAATTATTCATGTTGTTACACAGTAATTAGATCCATCAGTTAAAAGAAATTTGTTGTTCC

Intron 3 GTACTTGTGA ... TCTATTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	7	3
A2	92.73	0.4	4	4
A3	95.07	0.7	13.5	12
A4	93.39	0.4	6	3
A5	77.38	1.7	5	6
A6	81.37	11.9	44.7	58
A7	78.6	15.8	54.5	58
A8	76.8	24.5	71.6	79
A9	79.88	17.2	64.5	67
A10	77.93	8.9	27.4	32

>TRINITY_DN44040_c2_g5_i11 from sample A7 / Example spliced isoform

GAGTATTAAAGCAGTCCGAAGCCTTTGATCCGCCTCCGATATGCATTGAAGCAATATTCTTCTACGTTCGAAGCATGACATCTGCAGTGTCTAGCATATGGTCTGCGGTGGTGGGATATTGAAGCCACCTGAAACTCGCAGATCTGACCAGCATCCGAGGAAATCTAGGGTTGGCGATATGGGGCCTTCAAATAATATCT
AGTGTTTGCAAGCCAATGGTTTGGTCTGCCTCTGATATGCATTAAAGCAATATTCTTCTGTGTTCGAAGCATGACATCTGCAGTGTTTGGAATATGGTATGTGGTGGTGGGAGCTTGAAGCCACCTAAAACTCGCAGATCTGACCAGCATCCGAGGCAATATAGGGTTGGCCATATGCTTCCTTCAAATAATATCTAGGG
TTCGCAAGCCAATGGTCGGCCGCATAATCTGTTGTTGGCCTGGTTGTTCTGATTTCCTTCAAACCCACAAATTTCTTGCTCTATTTTTCCTTTCGGACTTTCAGAGATTCAATCCATCGGACTTGATGCAGATGAATTGAACTGGAAAATCCATTTTCGGTCCTCAAATTAAGGGAAGTTGTGGAATATACGAATTTGCA
AAAAGTTGCTTCAAGAACTGAACAAAGGGAAAAATCTTGCTTCTAGAAGTTGTGATATGGACATCAAAGGAATCACGAGTGGGATAAAACACCACTTACCAAATCAGTTCATCTTTTCATATCAACATTCTTGCAACCACTTCTCAGATCTTGCAAGGTAGTAAATACGTTTTCTATTTTGATATATCTAAATCAAGTAT
CATTCTACCATGAATTATGAATAAAGTAATAACAATTAGGATGATTTCACCGATATTCCATACTTCAGTCTTATAAGGTTG

>TRINITY_DN44040_c2_g5_i6 from sample A7 / Example unspliced isoform:

TTTAATATAGTTTTGGTTTCTCTTGTAGCAAGCCACATTATGAAATTACCCATTCTTTATGGACCTCGTAATTCATGTTTAGATCTATCAAGACTCTGCTTTCTTTATTTTAGTATTTATTTTTATTTATTTTTGGACATCAAAGGTATCACTTTCTGAAATTTGTAGTGTTGCTTGATGACGAAATTGATGATATTGTG
CTATATTCCGCAAGCTCTAATTGTATCCATATATTTTGCAGTTGCTTCAAGAACTGAACAAAGGGAAAAATCTTGCTTCTAGAAGTTGTGATATGGACATCAAAGGAATCACGgtacttgtgatttttgtcttagtgtttcagtgcagtctcaatattgttgaaatactgagaaaacaaaattaactgcctcaaattgtt
ctattacagAGTGGGATAAAACACCACTTACCAAATCAGTTCATCTTTTCATATCAACATTCTTGCAACCACTTCTCAGATCTTGCAAGGTAGTAAATACGTTTTCTATTTTGATATATCTAAATCAAGTATCATTCTACCATGAATTATGAATAAAGTAATAACAATTAGGATGATTTCACCGATATTCCATACTTCAG
TCTTATAAGGTTG

Intron 4 GTAAGTCCCG ... GGAAAGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10	9	2	0
A2	80	1	5	3
A3	16.67	10	1	3
A4	25	6	2	2
A5	33.33	9	4	5
A6	14.29	42	4	10
A7	34.29	23	9	15
A8	22.35	33	6	13
A9	24.68	29	6	13
A10	5.15	46	4	1

>TRINITY_DN44040_c2_g5_i3 from sample A7 / Example spliced isoform

GAGTATTAAAGCAGTCCGAAGCCTTTGATCCGCCTCCGATATGCATTGAAGCAATATTCTTCTACGTTCGAAGCATGACATCTGCAGTGTCTAGCATATGGTCTGCGGTGGTGGGATATTGAAGCCACCTGAAACTCGCAGATCTGACCAGCATCCGAGGAAATCTAGGGTTGGCGATATGGGGCCTTCAAATAATATCT
AGTGTTTGCAAGCCAATGGTTTGGTCTGCCTCTGATATGCATTAAAGCAATATTCTTCTGTGTTCGAAGCATGACATCTGCAGTGTTTGGAATATGGTATGTGGTGGTGGGAGCTTGAAGCCACCTAAAACTCGCAGATCTGACCAGCATCCGAGGCAATATAGGGTTGGCCATATGCTTCCTTCAAATAATATCTAGGG
TTCGCAAGCCAATGGTCGGCCGCATAATCTGTTGTTGGCCTGGTTGTTCTGATTTCCTTCAAACCCACAAATTTCTTGCTCTATTTTTCCTTTCGGACTTTCAGAGATTCAATCCATCGGACTTGATGCAGATGAATTGAACTGGAAAATCCATTTTCGGTCCTCAAATTAAGGGAAGTTGTGGAATATACGAATTTGCA
AAAAGGGCCCAATGTGCGCAACCGCCCATACAAATATTTTTGCAGTCGCTCAACCTGTGATTTTACATTCACTAGCTAGAATGGGAAGAGAAGATGTCGATGGAAGAGGCCCAACACCTGAAGGCATGACGATGGTACCCATGATACTTCCATATGGGCGTGTTGGATACATATTGTAAGTCTCACCATCAATTGGGTTT
AGTTCACCTCCACATCATTGACACACGAAAGGAAACTTGAGTCCCCCAGCAGTCTCACCATCAATCATGTTTCCTTCCCCTTCGAATTATCAACATGTGAAAGGACCCTTTGCTTCTCCTGCAAACATTTCCCCTTCGGAATCACCTTAGATGATGTGGAGGATACCTTTGGAACCACTGCCATTGGTATTCCCCCCTCC
CCCCCACCTACTAAAGAGATTGCGGTCCAATAGCTTGCACTCAACAATTGACTTCTACTCCTCTTGGTGCACCATGTGGTTGTGTATATCCAATGCAAGTGGAACTTGAGCTTGGGGTGGCACTTTGTGTTTTTTTCCCATTGGTTTCAGTGCTCGCTGCATAGATAGCAACATGAACTTTCTTGAAACAAATCCAAGTA
AGGATAATGGGAGCAAATGTAGATTCCCAAGATTAGGAAAAGACAATTGTGATTCTTGAT

>TRINITY_DN44040_c2_g5_i5 from sample A7 / Example unspliced isoform:

GAGTATTAAAGCAGTCCGAAGCCTTTGATCCGCCTCCGATATGCATTGAAGCAATATTCTTCTACGTTCGAAGCATGACATCTGCAGTGTCTAGCATATGGTCTGCGGTGGTGGGATATTGAAGCCACCTGAAACTCGCAGATCTGACCAGCATCCGAGGAAATCTAGGGTTGGCGATATGGGGCCTTCAAATAATATCT
AGTGTTTGCAAGCCAATGGTTTGGTCTGCCTCTGATATGCATTAAAGCAATATTCTTCTGTGTTCGAAGCATGACATCTGCAGTGTTTGGAATATGGTATGTGGTGGTGGGAGCTTGAAGCCACCTAAAACTCGCAGATCTGACCAGCATCCGAGGCAATATAGGGTTGGCCATATGCTTCCTTCAAATAATATCTAGGG
TTCGCAAGCCAATGGTCGGCCGCATAATCTGTTGTTGGCCTGGTTGTTCTGATTTCCTTCAAACCCACAAATTTCTTGCTCTATTTTTCCTTTCGGACTTTCAGAGATTCAATCCATCGGACTTGATGCAGATGAATTGAACTGGAAAATCCATTTTCGGTCCTCAAATTAAGGGAAGTTGTGGAATATACGAATTTGCA
AAAAGGGCCCAATGTGCGCAACCGCCCATACAAATATTTTTGCAGTCGCTCAACCTGTGATTTTACATTCACTgtaagtcccggtggaggagtcatgtatatctccttatgcatatcactttcaagtaacgcattataaacgtccatttgatacagtttcctgtggaaagacagAGCTAGAATGGGAAGAGAAGATGTCG
ATGGAAGAGGCCCAACACCTGAAGGCATGACGATGGTACCCATGATACTTCCATATGGGCGTGTTGGATACATATTGTAAGTCTCACCATCAATTGGGTTTAGTTCACCTCCACATCATTGACACACGAAAGGAAACTTGAGTCCCCCAGCAGTCTCACCATCAATCATGTTTCCTTCCCCTTCGAATTATCAACATGTG
AAAGGACCCTTTGCTTCTCCTGCAAACATTTCCCCTTCGGAATCACCTTAGATGATGTGGAGGATACCTTTGGAACCACTGCCATTGGTATTCCCCCCTCCCCCCCACCTACTAAAGAGATTGCGGTCCAATAGCTTGCACTCAACAATTGACTTCTACTCCTCTTGGTGCACCATGTGGTTGTGTATATCCAATGCAAG
TGGAACTTGAGCTTGGGGTGGCACTTTGTGTTTTTTTCCCATTGGTTTCAGTGCTCGCTGCATAGATAGCAACATGAACTTTCTTGAAACAAATCCAAGTAAGGATAATGGGAGCAAATGTAGATTCCCAAGATTAGGAAAAGACAATTGTGATTCTTGAT