sp|Q94AH6|CUL1_ARATH ----> Cullin-1

Intron 1 GTTAGGAAGA ... GGTTCTGGAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	4	2	0
A2	50	1	1	0
A3	0	1	0	0
A4	-1	0	0	0
A5	100	0	3	0
A6	100	0	2	0
A7	66.67	1	2	2
A8	50	7	7	4
A9	66.67	2	4	0
A10	100	0	2	0

>TRINITY_DN40912_c0_g1_i3 from sample A9 / Example spliced isoform

GCCGTAGAAATCTTTGGGAGAATAGCTAAAATAGGTGAACCAGATGTATTAAAATCTTAGGGTTGTGTGCCCCAAAATCCTGCAGCGCTCTTTTCTTTTCATCAGCGCTTTTTCATTAGCTATTATAAATACGGTTTGGTTCGGATTTTATTACACCTGCATGGCGTGGGTGGTGGGTGGTTAATGCCATTGTCCGCAAC
GGCAAGTATGGTGTTTCTCGTCTGCTTCAATGTTGGGTCCGACGGTATGGTGGGTGTCATTTCTACTACTGATGGTTGTCTCGACGGCATGTTTGCTCATGGCTTCGGCTTTTCACAGGTCTGTTTTGCGAGGTCGTGGATTTTGGAAGTGAAGCAAGGAGCGGTATGGCGGATGGGTGCCGTGGTGGAGAGAGGGCTCC
TGGTTTCCGCATTGCCCTTCGTCAAAACGCTAGCGTTCTGCGATTGACAGGCGGTCGGCCGGGACATGCAGTTCACTGTGCTGTCCGGTACATTTATTTCTTGGTTATGGGGCCGCGTGGTTATGGTGTCATTTCTATTTATTGCAGTTCACTGTGCAATTTGTGGCCCTTTGCAGAGCTATTTTCTCTTTCCGTGGTCT
TTTCCCTTGTCTGTATTTCCGACTCGCGTTGGCAAGATATTTTTTCGTTGGTGTTGTTTCTTGCTGAGGAAGGCGTCTCCTGTTGTCTGCGTTGTCATCGGTGCTTTGTTGCGTTTTCCCATTTGCTGCAACTTGGGTTGAAGAAGCCAGGCTCTGCCCCTGTTCCTGGTTGTTTCTGTTCATCGATGCTTGTCTGTGGA
TGCCGCTATTCGTGATTGCTCGTCCATGATCGTGCTATTAACAAAGAAACTTGCATCCGCGCTTCCAACTGTGGTCGTCGTGGCATCTTATCGGGGGTTTGCTTCGGCAATTTGACATTGGCTGTGAACTCTACGCCTCTGCCTATTTGATTAAACATGTTTGGATCATGTATGTTCACCTTGCAAACGAATTCAAAAAC
CTTTGGACACAGCATCACGGACCACAGCTTTTGGCACTGATACAAGCAGACATCCAACAATTTGCATATGGGTTTTTTTTAGGGCTTCAGGGCCCAATTCTCGTGGGGAGATGCTTTGTCTCCATCATTGTTTGTATAAGATTTGTGCTATCATACTTGACTCCTGTAGAGTAGGAAGATTCAGTTAGGCGGTTCTGGAG


GTTAGGAAGATTTCTGTGTCCAGATTGTGGGAAAGCTTTGTGACCGTCCTAGTTTTTAGCAAGCAGAATTTATGGCCCTATTAAGGCACTAATTCTGTGGTCTCTATCCATGTCAACGTTCTTAATAATCCATTTTTTTCTTCATCTTCGTATGGAAGTAGAATCTTAATCTTCTTCTTCGGGTTGTCAAACTGTAATTG
ACTTCAAATCCATCGCTCGGACAGATTTGTTTTGTATACGGCTCCTTGAGCAAAATTTTATAATTTCCAAATACTACAACTGAATGTAGTAGTACAAGATATTCATCTCCCAAATTCAATTGAGATTTGATGTCTAAATAACTCACCTTTTGAGAGTTACTAAACAATAACAATAAAATTGTATGGTATAGTGTTACCAA
CATTTCTATTGGCCCAGCATCAAACTTGCACATGACATTACAGGTCGTCATCAAATAGAGCGC

>TRINITY_DN40912_c0_g1_i4 from sample A9 / Example unspliced isoform:

ATTTGTGCTATCATACTTGACTCCTGTAGAGTAGGAAGATTCAGTTAGGCGGTTCTGGAGgttaggaagatttgtgctatcatacttgactcttgtagagaatgggcctcgagatttggttctggaggtggattgatgctctcttttttttctcggcttccaccaggactctagaggagactccgctcgaagcttttcca
ctgattttattttatttttctttcaaattccctttctttgttttttttgcaataactagctctgcagaatgccatcaatttcaacctcgttcctatatcttttttctttgtctactatcctctgattcagtgaaaaagctggttgcacaggtctcacgaaacatcgatggcattgcgcctgtagagtaggaagattcagt
taggcggttctggagGTTAGGAAGATTTCTGTGTCCAGATTGTGGGAAAGCTTTGTGACCGTCCTAGTTTTTAGCAAGCAGAATTTATGGCCCTATTAAGGCACTAATTCTGTGGTCTCTATCCATGTCAACGTTCTTAATAATCCATTTTTTTCTTCATCTTCGTATGGAAGTAGAATCTTAATCTTCTTCTTCGGGTT
GTCAAACTGTAATTGACTTCAAATCCATCGCTCGGACAGATTTGTTTTGTATACGGCTCCTTGAGCAAAATTTTATAATTTCCAAATACTACAACTGAATGTAGTAGTACAAGATATTCATCTCCCAAATTCAATTGAGATTTGATGTCTAAATAACTCACCTTTTGAGAGTTACTAAACAATAACAATAAAATTGTATG
GTATAGTGTTACCAACATTTCTATTGGCCCAGCATCAAACTTGCACATGACATTACAGGTCGTCATCAAATAGAGCGC

Intron 2 GTGGGTGTCA ... CTTTTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	14	16
A2	100	0	2	0
A3	96.23	1	28	23
A4	66.67	2	4	4
A5	87.5	2	11	17
A6	86.47	18	104	126
A7	81.55	25	104	117
A8	86.83	22.5	139.8	157
A9	82.63	27	122.8	134
A10	89.89	18	154	166

>TRINITY_DN46248_c1_g2_i1 from sample A8 / Example spliced isoform

GGATTTTATTACACCTGCATGGCGTGGGTGGTGGGTGGTTAATGCCATTGTCCGCAACGGCAAGTATGGTGTTTCTCGTCTGCTTCAATGTTGGGTCCGACGGTATGGTCTGTTTTGCGAGGTCGTGGATTTTGGAAGTGAAGCAAGGAGCGGTATGGCGGATGGGTGCCGTGGTGGAGAGAGGGCTCCTGGTTTCCGCA
TTGCCCTTCGTCAAAACGCTAGCGTTCTGCGATTGACAGGCGGTCGGCCGGGACATGCAGTTCACTGTGCTGTCCGGTACATTTATTTCTTGGTTATGGGGCCGCGTGGTTATGGTGTCATTTCTATTTATTGCAGTTCACTGTGCAATTTGTGGCCCTTTGCAGAGCTATTTTCTCTTTCCGTGGTCTTTTCCCTTGTC
TGTATTTCCGACTCGCGTTGGCAAGATATTTTTTCGTTGGTGTTGTTTCTTGCTGAGGAAGGCGTCTCCTGTTGTCTGCGTTGTCATCGGTGCTTTGTTGCGTTTTCCCATTTGCTGCAACTTGGGTTGAAGAAGCCAGGCTCTGCCCCTGTTCCTGGTTGTTTCTGTTCATCGATGCTTGTCTGTGGATGCCGCTATTC
GTGATTGCTCGTCCATGATCGTGCTATTAACAAAGAAACTTGCATCCGCGCTTCCAACTGTGGTCGTCGTGGCATCTTATCGGGAGCAAAGGGTCACAAAAACAATTGCTGGATGGGGTGACTTCTTGCAGGATTTTTTTTTTTGGTTGAGACAAAATACAGAGCTTTTTTTGTTACTGTTCACACAGCTTTTGCTTCCA
CATATTGCACAACTTCATGAGCAATTATTTTGAAACTTATCCACTGATCTTTTGATACCAAGTATATCTATAGCTAGTAGCATATACATATCAAATAGAGTTTTTTCCATTTGTATCAATGCTTTCAAACAATGTTTTCACAACAAATATATCTGCAGCTGATCACTTAGACAAAGCAAATAAATTTTTTCCTTTATATC
TATTATCATTACTACCATGAAATGCTTTTCCT

>TRINITY_DN46248_c1_g2_i3 from sample A8 / Example unspliced isoform:

GGATTTTATTACACCTGCATGGCGTGGGTGGTGGGTGGTTAATGCCATTGTCCGCAACGGCAAGTATGGTGTTTCTCGTCTGCTTCAATGTTGGGTCCGACGGTATGgtgggtgtcatttctactactgatggttgtctcgacggcatgtttgctcatggcttcggcttttcacagGTCTGTTTTGCGAGGTCGTGGATT
TTGGAAGTGAAGCAAGGAGCGGTATGGCGGATGGGTGCCGTGGTGGAGAGAGGGCTCCTGGTTTCCGCATTGCCCTTCGTCAAAACGCTAGCGTTCTGCGATTGACAGGCGGTCGGCCGGGACATGCAGTTCACTGTGCTGTCCGGTACATTTATTTCTTGGTTATGGGGCCGCGTGGTTATGGTGTCATTTCTATTTAT
TGCAGTTCACTGTGCAATTTGTGGCCCTTTGCAGAGCTATTTTCTCTTTCCGTGGTCTTTTCCCTTGTCTGTATTTCCGACTCGCGTTGGCAAGATATTTTTTCGTTGGTGTTGTTTCTTGCTGAGGAAGGCGTCTCCTGTTGTCTGCGTTGTCATCGGTGCTTTGTTGCGTTTTCCCATTTGCTGCAACTTGGGTTGAA
GAAGCCAGGCTCTGCCCCTGTTCCTGGTTGTTTCTGTTCATCGATGCTTGTCTGTGGATGCCGCTATTCGTGATTGCTCGTCCATGATCGTGCTATTAACAAAGAAACTTGCATCCGCGCTTCCAACTGTGGTCGTCGTGGCATCTTATCGGGAGCAAAGGGTCACAAAAACAATTGCTGGATGGGGTGACTTCTTGCAG
GATTTTTTTTTTTGGTTGAGACAAAATACAGAGCTTTTTTTGTTACTGTTCACACAGCTTTTGCTTCCACATATTGCACAACTTCATGAGCAATTATTTTGAAACTTATCCACTGATCTTTTGATACCAAGTATATCTATAGCTAGTAGCATATACATATCAAATAGAGTTTTTTCCATTTGTATCAATGCTTTCAAACA
ATGTTTTCACAACAAATATATCTGCAGCTGATCACTTAGACAAAGCAAATAAATTTTTTCCTTTATATCTATTATCATTACTACCATGAAATGCTTTTCCT