NA ----> NA

Intron 1 GTACCTATTT ... ACCATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	68.18	7	2	15
A2	36.36	7	0	4
A3	55.26	17	0	21
A4	50	8	0	8
A5	63.16	7	0	12
A6	47.37	20	0	18
A7	50	30	0	30
A8	36.11	23	0.8	13
A9	36	32	0.8	18
A10	63.64	12	5	21

>TRINITY_DN100631_c0_g1_i2 from sample A1 / Example spliced isoform

ATTTTGACGTGCTGTGACATGTTAATTTTGAACCGTGGGTGACCAATACCACCCAATACCATTGACTAAACTTCATCGGTAGCAGGGATTCATTTGTCGAAGGAATCGGGATCAACAATGGCAATGGCAGTCGCCCATCACTCATACCCTTCCCGAGCACTGTCAGACAGCGATTGTGCCATCAATGGCAATGGCAGCCA
AATGTGGGGAAAATGCAAATGCCTCAGATCGACCCCCGGGGCGAGAGCGAGGTTTCCTCCTAACAGAATGGTGAGAATGGGATTGAGGGAGGATGGGCCCTCGGTGGCTGTTGTGGGTGTCACGGGTGCCGTCGGGCAGGAGTTCTTGAGCGTGTTGAGTGATAGGGATTTTCCCTACAGAAGTCTCAGATTGCTGGCTA
GCAAGCGCTCTGCTGGAAAGCAAATGACATTTGAGGGACGAGACTATGCAGTAGAGGAGCTCACTGAAGATAGTTTCCACGGGATTGACATTGCTCTGTTCAGTGCTGGTGGCTCCATAAGCAAAAAATTTGGACCCATAGCTGCACAACAGGGGACAATTGTAGTAGACAATAGCTCTGCATTCCGTATGGACGATGGT
GTACCCCTTGTGATCCCCGAGGTGAATCCAGAGGCAATGTCTCACATAAAACTTAGTTATAAGAAGGGAGCGCTTATTGCGAACCCAAACTGTTCAACTATCATCTGTCTCATGGCAGCAACGCCTCTCCACCGTCATGCCAAGGTGAAAAGGATGGTGGTGAGCACATATCAGGCAGCCAGTGGAGCTGGTGCTGCAGC
TATGGCTGAGCTTGAACAACAAACCCGCGAGGTTTTAGAAGGAAAAGAGCCAACCTGTAAAATCTTCAGTCAACAGTATGCATTCAACCTCTTTTCACACAACTCACAAGTCTCCCCAGATGGTTACAATGAGGAGGAGCTGAAGTTAGTCAAAGAGACCAAGAAAATTTGGAATGACCAAGATGTGAGAGTCACTGCCA
CTTGTGTACGGGTCCCAGTAATGCGGGCACATGCAGAGAGCATAAATTTCGAATTTGAGAAACCACTCAATGAGGATACTGCAAGAGAGATTCTTAAGAAGGCTGCTGGGGTTGTTGTCATTGATGATCGAGCTGCAAACATCTTTCCCACACCCCTTGAGGTTTCAAATAAAGATGATGTTGCTGTTGGTAGGATACGG
CAAGATGTTTCTCAAAATGGTGATTATGGATTGGATATTTTCATTTGTGGGGATCAAATACGCAAAGGAGCTGCTCTAAATGCAGTACAAATTGCAGAGAGGCTACTATAAGAGTTTTAAGATCTGAATAACATATTGACGACCTGTTATTCCATGCAGTTCTTTTGAGTATTTTGTTTCCGACAGGAGAAATTTCCTAG
ATGATTATAGAGCAATTAATTAAGGCATTACATTTGATTAGCTTCTTAGCCCAATTCTGTCACCTTGTGATATGCCGGTAACAAATGGTGTACCTTTGAGGAAAAGAGTATTGAGAATAGCCATTCTGTGGTGAAATGGTTTTTGAGGATATAGTAAACCTTGGTTTTAGATGGAAAATTTAAAAACTGCTGGTGGAGGG
TGATGTGATTGTACATCATATCTCTCTCAGAAGACCCCTTTGTGATTCTTGTTTAACATGTTTTTATGGAATTCCTTTGGGTTTTCATTTTTTACAAGATTCTTTTTATCTTAAAA

>TRINITY_DN100631_c0_g1_i1 from sample A1 / Example unspliced isoform:

ATTTTGACGTGCTGTGACATGTTAATTTTGAACCGTGGGTGACCAATACCACCCAATACCATTGACTAAACTTCATCGgtacctatttctcttcttcatttccagatagctatttgtttattcacgtaacgttttctcgttccctactgcccagaactatactaaagagaagcacagttaaccattgcagGTAGCAGGGA
TTCATTTGTCGAAGGAATCGGGATCAACAATGGCAATGGCAGTCGCCCATCACTCATACCCTTCCCGAGCACTGTCAGACAGCGATTGTGCCATCAATGGCAATGGCAGCCAAATGTGGGGAAAATGCAAATGCCTCAGATCGACCCCCGGGGCGAGAGCGAGGTTTCCTCCTAACAGAATGGTGAGAATGGGATTGAGG
GAGGATGGGCCCTCGGTGGCTGTTGTGGGTGTCACGGGTGCCGTCGGGCAGGAGTTCTTGAGCGTGTTGAGTGATAGGGATTTTCCCTACAGAAGTCTCAGATTGCTGGCTAGCAAGCGCTCTGCTGGAAAGCAAATGACATTTGAGGGACGAGACTATGCAGTAGAGGAGCTCACTGAAGATAGTTTCCACGGGATTGA
CATTGCTCTGTTCAGTGCTGGTGGCTCCATAAGCAAAAAATTTGGACCCATAGCTGCACAACAGGGGACAATTGTAGTAGACAATAGCTCTGCATTCCGTATGGACGATGGTGTACCCCTTGTGATCCCCGAGGTGAATCCAGAGGCAATGTCTCACATAAAACTTAGTTATAAGAAGGGAGCGCTTATTGCGAACCCAA
ACTGTTCAACTATCATCTGTCTCATGGCAGCAACGCCTCTCCACCGTCATGCCAAGGTGAAAAGGATGGTGGTGAGCACATATCAGGCAGCCAGTGGAGCTGGTGCTGCAGCTATGGCTGAGCTTGAACAACAAACCCGCGAGGTTTTAGAAGGAAAAGAGCCAACCTGTAAAATCTTCAGTCAACAGTATGCATTCAAC
CTCTTTTCACACAACTCACAAGTCTCCCCAGATGGTTACAATGAGGAGGAGCTGAAGTTAGTCAAAGAGACCAAGAAAATTTGGAATGACCAAGATGTGAGAGTCACTGCCACTTGTGTACGGGTCCCAGTAATGCGGGCACATGCAGAGAGCATAAATTTCGAATTTGAGAAACCACTCAATGAGGATACTGCAAGAGA
GATTCTTAAGAAGGCTGCTGGGGTTGTTGTCATTGATGATCGAGCTGCAAACATCTTTCCCACACCCCTTGAGGTTTCAAATAAAGATGATGTTGCTGTTGGTAGGATACGGCAAGATGTTTCTCAAAATGGTGATTATGGATTGGATATTTTCATTTGTGGGGATCAAATACGCAAAGGAGCTGCTCTAAATGCAGTAC
AAATTGCAGAGAGGCTACTATAAGAGTTTTAAGATCTGAATAACATATTGACGACCTGTTATTCCATGCAGTTCTTTTGAGTATTTTGTTTCCGACAGGAGAAATTTCCTAGATGATTATAGAGCAATTAATTAAGGCATTACATTTGATTAGCTTCTTAGCCCAATTCTGTCACCTTGTGATATGCCGGTAACAAATGG
TGTACCTTTGAGGAAAAGAGTATTGAGAATAGCCATTCTGTGGTGAAATGGTTTTTGAGGATATAGTAAACCTTGGTTTTAGATGGAAAATTTAAAAACTGCTGGTGGAGGGTGATGTGATTGTACATCATATCTCTCTCAGAAGACCCCTTTGTGATTCTTGTTTAACATGTTTTTATGGAATTCCTTTGGGTTTTCAT
TTTTTACAAGATTCTTTTTATCTTAAAA

Intron 2 GTTAGTGTTA ... TTGTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.04	76	41	0
A2	31.25	33	15	2
A3	32.26	63	30	4
A4	21.82	43	12	1
A5	33.33	22	11	2
A6	31.15	126	57	0
A7	31.09	133	60	0
A8	25.97	57	20	0
A9	31.11	93	42	0
A10	32.85	92	45	0

>TRINITY_DN105960_c0_g1_i1 from sample A3 / Example spliced isoform

CTATTTCTCTTCTTCATTTCCAGATAGCTATTTGTTTATTCACGTAACGTTTTCTCGTTCCCTACTGCCCAGAACTATACTAAAGAGAAGCACAGTTAACCATTGCAGGTAGCAGGGATTCATTTGTCGAAGGAATCGGGATCAACAATGGCAATGGCAGTCGCCCATCACTCATACCCTTCCCGAGCACTGTCAGACAG
CGATTGTGCCATCAATGGCAATGGCAGCCAAATGTGGGGAAAATGCAAATGCCTCAGATCGACCCCCGGGGCGAGAGCGAGGTTTCCTCCTAACAGAATGGTGAGAATGGGATTGAGGGAGGATGGGCCCTCGGTGGCTGTTGTGGGTGTCACGGGTGCCGTCGGGCAGGAGTTCTTGAGCGTGTTGAGTGATAGGGATT
TTCCCTACAGAAGTCTCAGATTGCTGGCTAGCAAGCGCTCTGCTGGAAAGCAAATGACATTTGAGGGACGAGACTATGCAGTAGAGGAGCTCACTGAAGATAGTTTCCACGGGATTGACATTGCTCTGTTCAGTGCTGGTGGCTCCATAAGCAAAAAATTTGGACCCATAGCTGCACAACAGGGGACAATTGTAGTAGAC
AATAGCTCTGCATTCCGTATGGACGATGGTGTACCCCTTGTGATCCCCGAGGTGAATCCAGAGGCAATGTCTCACATAAAACTTAGTTATAAGAAGGGAGCGCTTATTGCGAACCCAAACTGTTCAACTATCATCTGTCTCATGGCAGCAACGCCTCTCCACCGTCATGCCAAGGTGAAAAGGATGGTGGTGAGCACATA
TCAGGCAGCCAGTGGAGCTGGTGCTGCAGCTATGGCTGAGCTTGAACAACAAACCCGCGAGGTTTTAGAAGGAAAAGAGCCAACCTGTAAAATCTTCAGTCAACAGTATGCATTCAACCTCTTTTCACACAACTCACAAGTCTCCCCAGATGGTTACAATGAGGAGGAGCTGAAGTTAGTCAAAGAGACCAAGAAAATTT
GGAATGACCAAGATGTGAGAGTCACTGCCACTTGTGTACGGGTCCCAGTAATGCGGGCACATGCAGAGAGCATAAATTTCGAATTTGAGAAACCACTCAATGAGGTAATCCACTATCAGTATTTTATACTTAATTTTGTTTCTTGGAAAATGCATATAATAAAGATAGATGGTGTTTATCTTGGGGTTTTTCCT

>TRINITY_DN105960_c0_g1_i2 from sample A3 / Example unspliced isoform:

CTATTTCTCTTCTTCATTTCCAGATAGCTATTTGTTTATTCACGTAACGTTTTCTCGTTCCCTACTGCCCAGAACTATACTAAAGAGAAGCACAGTTAACCATTGCAGGTAGCAGGGATTCATTTGTCGAAGGAATCGGGATCAACAATGGCAATGGCAGTCGCCCATCACTCATACCCTTCCCGAGCACTGTCAGACAG
CGATTGTGCCATCAATGGCAATGGCAGCCAAATGTGGGGAAAATGCAAATGCCTCAGATCGACCCCCGGGGCGAGAGCGAGGTTTCCTCCTAACAGAATGGTGAGAATGGGATTGAGGGAGGATGGGCCCTCGGTGGCTGTTGTGGGTGTCACGGGTGCCGTCGGGCAGGAGTTCTTGAGCGTGTTGAGTGATAGGGATT
TTCCCTACAGAAGTCTCAGATTGCTGGCTAGCAAGCGCTCTGCTGGAAAGCAAATGACATTTGAGgttagtgttaatatcacaggacaaatcgttggcacaaaatgggtaccttagcttgcatttggttgacattgtgtacagGGACGAGACTATGCAGTAGAGGAGCTCACTGAAGATAGTTTCCACGGGATTGACATT
GCTCTGTTCAGTGCTGGTGGCTCCATAAGGTCAATATTATATTTCGGATGAATATACTATCTGGGAATCAAGTTTCCACTAATGGGAGTG