sp|Q9ZV24|OP161_ARATH ----> Outer envelope pore protein 16-1, chloroplastic

Intron 1 GTACAAATTC ... CATTGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.22	4	2	13
A2	80.95	2	12	5
A3	88.24	1	11	4
A4	50	5	4	6
A5	50	2	4	0
A6	34.78	15	6	10
A7	44	28	18	26
A8	57.69	11	17	13
A9	50	16	15	17
A10	53.7	25	36	22

>TRINITY_DN101628_c0_g3_i1 from sample A4 / Example spliced isoform

CGGCATTTGGCAAATCCCATCTTAACATAACGTAGAACTGTAGAACCGATCTGTAATAGTAATTGCAAATCCCATGATTAAGATTTCGTTCACATGAACACTGTGCACATTGGACGCTTCTATCCCGAAAAATGGGGCTGTTTAAAGCTCTTGTCTCCACTAACACTGACAGAAATATATAAGAGTGAAGCTTTTCTCAG
GCCAAGTACTCACTTCTGCTCTGCTACCGTGACAAATTCCTAGAGATTCCAAGTTTCTTTACAGTTTTTCGCAGGTCTGCATACTTCCGAATTGCTGCACTTGAGCACCCATTCGGAGCACCTGACAAGATGCCTTCTGGAAGAATTTCTGCCAACGTTCATTCGCCTACTGTGGGGGTGATGATTGATATGGGCAACCC
TTTGCTTAATCGAACACTTGACGGATTTCTCAAAATTGGAACTGTGGGTGCTTCGCGCGTCGTTGTTGAAGAGACTTACGGCTGTTTGAGTAAAGGAAAAGTGTCAAAGCATGATCTTGAGCATACGCTCAAGGAGATGGGAAAGGAGGGGTTACGCTGGGGAGCTGTAGCTGGTGTATACACTGGAGTAGAATATGGAG
TAGAAAGAATTCGTGGCAGGCGAGACTGGACGAATGCTTTAATAAGTGGTGCTCTGACTGGAGCAGCCGTGTCCTTCAGTGATAATAAGTACAGCAGAGACAAGATGATGAAGGACGCCATTATGGGAGGAGCCATAGCCACTGCTGCCGAATTTATAAGATATCTCACATAAAACAACAGATTTCCAGCGAACTTAGTG
AAAGATTATACACGATGACATAAATTTTCTGAGCATTACTCTGCTTGCCATATATGCAGTCCCCGGATTGTTTAAATAAAAGGAATTTAATATGACTAATGAATTGGTAGACCGAATGCTTCAAATTTGTGAAAAAAAGAAAAAACAACCTTTGTTATCG

>TRINITY_DN101628_c0_g3_i2 from sample A4 / Example unspliced isoform:

CGGCATTTGGCAAATCCCATCTTAACATAACGTAGAACTGTAGAACCGATCTGTAATAGTAATTGCAAATCCCATGATTAAGATTTCGTTCACATGAACACTGTGCACATTGGACGCTTCTATCCCGAAAAATGGGGCTGTTTAAAGCTCTTGTCTCCACTAACACTgtacaaattcctagtttaggggcatcccagatt
tttattacttcacttcctcgacacgcatctacaaagcacaatccattttttgtttttttcgacatcacaaatcattaaattaaataatatctcacatttcattgcacagGACAGAAATATATAAGAGTGAAGCTTTTCTCAGGCCAAGTACTCACTTCTGCTCTGCTACCGTGACAAATTCCTAGAGATTCCAAGTTTCT
TTACAGTTTTTCGCAGGTCTGCATACTTCCGAATTGCTGCACTTGAGCACCCATTCGGAGCACCTGACAAGATGCCTTCTGGAAGAATTTCTGCCAACGTTCATTCGCCTACTGTGGGGGTGATGATTGATATGGGCAACCCTTTGCTTAATCGAACACTTGACGGATTTCTCAAAATTGGAACTGTGGGTGCTTCGCGC
GTCGTTGTTGAAGAGACTTACGGCTGTTTGAGTAAAGGAAAAGTGTCAAAGCATGATCTTGAGCATACGCTCAAGGAGATGGGAAAGGAGGGGTTACGCTGGGGAGCTGTAGCTGGTGTATACACTGGAGTAGAATATGGAGTAGAAAGAATTCGTGGCAGGCGAGACTGGACGAATGCTTTAATAAGTGGTGCTCTGAC
TGGAGCAGCCGTGTCCTTCAGTGATAATAAGTACAGCAGAGACAAGATGATGAAGGACGCCATTATGGGAGGAGCCATAGCCACTGCTGCCGAATTTATAAGATATCTCACATAAAACAACAGATTTCCAGCGAACTTAGTGAAAGATTATACACGATGACATAAATTTTCTGAGCATTACTCTGCTTGCCATATATGCA
GTCCCCGGATTGTTTAAATAAAAGGAATTTAATATGACTAATGAATTGGTAGACCGAATGCTTCAAATTTGTGAAAAAAAGAAAAAACAACCTTTGTTATCG

Intron 2 GTACAAATTC ... CACAGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.54	5	2	14
A2	70.37	4	12	7
A3	80	2	11	5
A4	46.15	7	4	8
A5	50	2	4	0
A6	34.48	19	6	14
A7	42.61	33	18	31
A8	54.1	14	17	16
A9	45.05	25	15	26
A10	50.43	29	36	23

>TRINITY_DN111570_c0_g3_i6 from sample A2 / Example spliced isoform

GGCAAATCCCATCTTAACATAACGTAGAACTGTAGAACCGATCTGTAATAGTAATTGCAAATCCCATGATTAAGATTTCGTTCACATGAACACTGTGCACATTGGACGCTTCTATCCCGAAAAATGGGGCTGTTTAAAGCTCTTGTCTCCACTAACACTAAATATATAAGAGTGAAGCTTTTCTCAGGCCAAGTACTCAC
TTCTGCTCTGCTACCGTGACAAATTCCTAGAGATTCCAAGTTTCTTTACAGTTTTTCGCAGGTCTGCATACTTCCGAATTGCTGCACTTGAGCACCCATTCGGAGCACCTGACAAGATGCCTTCTGGAAGAATTTCTGCCAACGTTCATTCGCCTACTGTGGGGGTGATGATTGATATGGGCAACCCTTTGCTTAATCGA
ACACTTGACGGATTTCTCAAAATTGGAACTGTGGGTGCTTCGCGCGTCGTTGTTGAAGAGACTTACGGCTGTTTGAGTAAAGGAAAAGTGTCAAAGCATGATCTTGAGCATACGCTCAAGGAGATGGGAAAGGAGGGGTTACGCTGGGGAGCTGTAGCTGGTGTATACACTGGAGTAGAATATGGAGTAGAAAGAATTCG
TGGCAGGCGAGACTGGACGAATGCTTTAATAAGTGGTGCTCTGACTGGAGCAGCCGTGTCCTTCAGTGATAATAAGTACAGCAGAGACAAGATGATGAAGGACGCCATTATGGGAGGAGCCATAGCCACTGCTGCCGAATTTATAAGATATCTCACATAAAACAACAGATTTCCAGCGAACTTAGTGAAAGATTATACAC
GATGACATAAATTTTCTGAGCATTACTCTGCTTGCCATATATGCAGTCCCCGGATTGTTTAAATAAAAGGAATTTAATATGACTAATGAATTGGTAGACCGAATGCTTCAAATTTGTGAAAAAAAGAAAAAACAACCTTTGTTATCGTTCATTTCCTTTGATATTTTGTGCATGCTGCATAAATGCATTGTTGTTACCAT
ATGTTAGTTTCATAGTCACAAGTTTGAAGAAGAATTGCATTGGAGAAAGGAGGTATCACTTCTTTTTGAAGTTAGCTATAGACATTCCTATGATCTGCATATACAAGTCACCTTTATATTTTGTCAGAGAATCAAATAATCTCTATGTTTACTCATAATCAAATAAACTCTTGATATATTTTTATTAGATGCATATTTTG
AAAGCACAAGGATTGGCTTTCCAATTTTTTGATATAATGCAATGTAGGTCATCTTGTTGTATTGCAAGTATAGTCAATCATGGTGTTTTTAGATACATGGCCTAAACTTATTTTGGATAGTTTTAAACTAGGTTTATTAGAAGTAACATATATATCATCTCAATATATCTTAATAGATATTTATTCCTTCTTGAGTACAA
CGTGAAAGTGAAATATACAGGCAAACCTATTGAAATACATCCAGAGATGTTGCAGTGAAAGCATTTGTGCATACATGAGAGGCATGTGATATACATGATAGGGACTTTCTTTACAACTGTATGAAACTTGAAGGCCCAAGTATCCAATTAGAATATATGAAATTGCTTTCTTCTATACCTAGGCCTCCTAAAGCCATAAT
GTGTATTGGTAAAAACTATGCTGACCATGTTAAATAAATTTATACATGGAAATGGCTTTTGGCATAGCTTCTCCCAATATACCAAAGTATCTTGTCATCCTCATAAATGCACCACAGTTAGTTATTTGACCAAGAGATGCAATAAGATATCCTCATTCAATAACAACCAAGTGGACTCTGAAACTGTGATTATTGAAAAA
TATGGGCATGGCATAAAGAGGGATGATGGAATGTCACATCCTTTTGGGTATACTATACTAAATGATGTAACTTAAAGAGACTTGCAAAAGCAT

>TRINITY_DN111570_c0_g3_i4 from sample A2 / Example unspliced isoform:

GGCAAATCCCATCTTAACATAACGTAGAACTGTAGAACCGATCTGTAATAGTAATTGCAAATCCCATGATTAAGATTTCGTTCACATGAACACTGTGCACATTGGACGCTTCTATCCCGAAAAATGGGGCTGTTTAAAGCTCTTGTCTCCACTAACACTgtacaaattcctagtttaggggcatcccagatttttattac
ttcacttcctcgacacgcatctacaaagcacaatccattttttgtttttttcgacatcacaaatcattaaattaaataatatctcacatttcattgcacaggacagAAATATATAAGAGTGAAGCTTTTCTCAGGCCAAGTACTCACTTCTGCTCTGCTACCGTGACAAATTCCTAGAGATTCCAAGTTTCTTTACAGTT
TTTCGCAGGTCTGCATACTTCCGAATTGCTGCACTTGAGCACCCATTCGGAGCACCTGACAAGATGCCTTCTGGAAGAATTTCTGCCAACGTTCATTCGCCTACTGTGGGGGTGATGATTGATATGGGCAACCCTTTGCTTAATCGAACACTTGACGGATTTCTCAAAATTGGAACTGTGGGTGCTTCGCGCGTCGTTGT
TGAAGAGACTTACGGCTGTTTGAGTAAAGGAAAAGTGTCAAAGCATGATCTTGAGCATACGCTCAAGGAGATGGGAAAGGAGGGGTTACGCTGGGGAGCTGTAGCTGGTGTATACACTGGAGTAGAATATGGAGTAGAAAGAATTCGTGGCAGGCGAGACTGGACGAATGCTTTAATAAGTGGTGCTCTGACTGGAGCAG
CCGTGTCCTTCAGTGATAATAAGTACAGCAGAGACAAGATGATGAAGGACGCCATTATGGGAGGAGCCATAGCCACTGCTGCCGAATTTATAAGATATCTCACATAAAACAACAGATTTCCAGCGAACTTAGTGAAAGATTATACACGATGACATAAATTTTCTGAGCATTACTCTGCTTGCCATATATGCAGTCCCCGG
ATTGTTTAAATAAAAGGAATTTAATATGACTAATGAATTGGTAGACCGAATGCTTCAAATTTGTGAAAAAAAGAAAAAACAACCTTTGTTATCGTTCATTTCCTTTGATATTTTGTGCATGCTGCATAAATGCATTGTTGTTACCATATGTTAGTTTCATAGTCACAAGTTTGAAGAAGAATTGCATTGGAGAAAGGAGG
TATCACTTCTTTTTGAAGTTAGCTATAGACATTCCTATGATCTGCATATACAAGTCACCTTTATATTTTGTCAGAGAATCAAATAATCTCTATGTTTACTCATAATCAAATAAACTCTTGATATATTTTTATTAGATGCATATTTTGAAAGCACAAGGATTGGCTTTCCAATTTTTTGATATAATGCAATGTAGGTCATC
TTGTTGTATTGCAAGTATAGTCAATCATGGTGTTTTTAGATACATGGCCTAAACTTATTTTGGATAGTTTTAAACTAGGTTTATTAGAAGTAACATATATATCATCTCAATATATCTTAATAGATATTTATTCCTTCTTGAGTACAACGTGAAAGTGAAATATACAGGCAAACCTATTGAAATACATCCAGAGATGTTGC
AGTGAAAGCATTTGTGCATACATGAGAGGCATGTGATATACATGATAGGGACTTTCTTTACAACTGTATGAAACTTGAAGGCCCAAGTATCCAATTAGAATATATGAAATTGCTTTCTTCTATACCTAGGCCTCCTAAAGCCATAATGTGTATTGGTAAAAACTATGCTGACCATGTTAAATAAATTTATACATGGAAAT
GGCTTTTGGCATAGCTTCTCCCAATATACCAAAGTATCTTGTCATCCTCATAAATGCACCACAGTTAGTTATTTGACCAAGAGATGCAATAAGATATCCTCATTCAATAACAACCAAGTGGACTCTGAAACTGTGATTATTGAAAAATATGGGCATGGCATAAAGAGGGATGATGGAATGTCACATCCTTTTGGGTATAC
TATACTAAATGATGTAACTTAAAGAGACTTGCAAAAGCAT