sp|Q9LJ86|LTPG5_ARATH ----> Non-specific lipid transfer protein GPI-anchored 5

Intron 1 GTGCTGATGT ... GATGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.94	460.2	194	180.8
A2	29.17	362	173	125.2
A3	31.19	467.2	269	154.6
A4	27.99	355	137	139
A5	28.8	361	157	135
A6	29.33	12052.8	5771	4233.2
A7	29.54	7931.6	3888.8	2761
A8	29.63	8492.2	4122.8	3028.6
A9	29.29	8760.8	4172.6	3085.8
A10	30.09	6262.8	3387.8	2003.4

>TRINITY_DN105542_c0_g1_i2 from sample A3 / Example spliced isoform

TTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTTCTGGTTCAGAGCCACGAAAATCAATTGCAATG
GCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTTACGGGCCTGAGCAACGACTCCAACCCAATATG
CTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCT
TTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGTAAGATTACACT
TAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAGTACTTGTTTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGGCAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGGTTTTTTCTTTT
AGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTC

>TRINITY_DN105542_c0_g1_i1 from sample A3 / Example unspliced isoform:

TTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTTCTGGTTCAGAGCCACGAAAATCAATTGCAATG
GCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTTACGGGCCTGAGCAACGACTCCAACCCAATATG
CTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGgtgctgatgttccgtacaagccgcccaccacaggtctgccag
tgacgcctccaaatgctgtcccaggcaggatctcagggttttctttggtccattacccattctttaatttctttgtaaattcttattttcttagctttgttgctctgatgtttcagGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGG
GCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGTAAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAG
TACTTGTTTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGGCAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCT
ACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTC

Intron 2 GTACACAATT ... CTAACTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.79	237.2	99	0
A2	32.06	208.4	97	0
A3	32.25	316.4	149	4
A4	33.89	155	79	0
A5	32.82	186.4	90	1
A6	30.56	7074.6	3062	5
A7	29.36	4858.6	1979	9
A8	30.35	5011.6	2150	9
A9	30.6	5106	2215	10
A10	30.62	4037.2	1759	5

>TRINITY_DN42218_c0_g1_i1 from sample A7 / Example spliced isoform

TGGAAAAAACAGAAAGAGTGGAGGCGGGTTTGTTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTT
CTGGTTCAGAGCCACGAAAATCAATTGCAATGGCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTT
ACGGGCCTGAGCAACGACTCCAACCCAATATGCTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGGTGCTGATGT
TCCGTACAAGCCGCCCACCACAGGTCTGCCAGTGACGCCTCCAAATGCTGTCCCAGGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTC
ATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGTAAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAGTACTTGTGTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAG
TTTTTCTTTTGTGCATGGCAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTA
CTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTCATCCA

>TRINITY_DN42218_c0_g1_i3 from sample A7 / Example unspliced isoform:

TGGAAAAAACAGAAAGAGTGGAGGCGGGTTTGTTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTT
CTGGTTCAGAGCCACGAAAATCAATTGCAATGGCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTT
ACGGGCCTGAGCAACGACTCCAACCCAATATGCTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGgtacacaatt
aaaccctaatactagaaagcttttaacaatgccaaaggctttcgcgtgatctttttcttgtttactgttactaactgcagGTGCTGATGTTCCGTACAAGCCGCCCACCACAGGTCTGCCAGTGACGCCTCCAAATGCTGTCCCAGGCAGGATCTCAGGGTTTTCTTTGGTCCATTACCCATTCTTTAATTTCTTTGTAA
ATTCTGATTTTCTTAGCTTTGTTGCTCTGATGTTTCAGGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGG
GTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGTAAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAGTACTTGTGTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGG
CAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAA
TTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTCATCCA

Intron 3 GCAGGATCTC ... GATGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.95	351.9	33.4	180.9
A2	33.79	245.1	21.6	125.1
A3	30.21	357.5	39.4	154.8
A4	38.2	224.9	17.4	139
A5	36.4	235.9	19.6	135
A6	31.86	9046.8	870.4	4230.1
A7	31.14	6106.5	587.6	2761.5
A8	32.37	6323.1	609.6	3027.3
A9	32.36	6447.1	620.2	3084.4
A10	30.78	4504.8	519.4	2002.7

>TRINITY_DN105542_c0_g1_i3 from sample A3 / Example spliced isoform

TTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTTCTGGTTCAGAGCCACGAAAATCAATTGCAATG
GCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTTACGGGCCTGAGCAACGACTCCAACCCAATATG
CTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGGTGCTGATGTTCCGTACAAGCCGCCCACCACAGGTCTGCCAG
TGACGCCTCCAAATGCTGTCCCAGGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGGGTGCCCTGTCCAGA
CCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGTAAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAGTACTTGTTTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGGCAATGAAGCTGGAA
AACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAG
ATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTC

>TRINITY_DN105542_c0_g1_i1 from sample A3 / Example unspliced isoform:

TTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTTCTGGTTCAGAGCCACGAAAATCAATTGCAATG
GCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTTACGGGCCTGAGCAACGACTCCAACCCAATATG
CTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGGTGCTGATGTTCCGTACAAGCCGCCCACCACAGGTCTGCCAG
TGACGCCTCCAAATGCTGTCCCAGgcaggatctcagggttttctttggtccattacccattctttaatttctttgtaaattcttattttcttagctttgttgctctgatgtttcagGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGG
GCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGTAAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAG
TACTTGTTTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGGCAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCT
ACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTC

Intron 4 GTGCTGATGT ... GCTGTCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40.2	460.2	266	352.8
A2	39.73	362	232	245.2
A3	43	467.2	348	357
A4	35.56	355	167	224.8
A5	37.96	361	206	235.8
A6	41.33	12052.8	7942	9042.6
A7	42.26	7931.6	5504.8	6105
A8	41.25	8492.2	5603.8	6322.2
A9	40.98	8760.8	5727.6	6442.2
A10	41.74	6262.8	4469.8	4504.6

>TRINITY_DN110712_c0_g1_i1 from sample A1 / Example spliced isoform

GCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTTCTGGTTCAGAGCCACGAAAATCAATTGCAATGGCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGC
TCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTTACGGGCCTGAGCAACGACTCCAACCCAATATGCTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTT
TTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGC
TCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGTAAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAGTACTTGTGTTTTC
GGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGGCAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCC
CTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTCATCCAATGTTATTTAAATAATAGTATTTTTACTATAAGGGATGATAATATTTTATAAAATTAAATAATAGTATTTTTACT
AGAAGGGATGATAATATTTTATAAAATTAAATAATAGTATTTTTACTAGAAGGGATGATAATATTTTATAAA

>TRINITY_DN110712_c0_g1_i2 from sample A1 / Example unspliced isoform:

GCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTTCTGGTTCAGAGCCACGAAAATCAATTGCAATGGCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGC
TCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTTACGGGCCTGAGCAACGACTCCAACCCAATATGCTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTT
TTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGgtgctgatgttccgtacaagccgcccaccacaggtctgccagtgacgcctccaaatgctgtcccagGTTCGATTCCTGCGCCCCCAACAACACCGCC
TGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGT
AAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAGTACTTGTGTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGGCAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGG
TTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTCATCCAATGTTATTT
AAATAATAGTATTTTTACTATAAGGGATGATAATATTTTATAAAATTAAATAATAGTATTTTTACTAGAAGGGATGATAATATTTTATAAAATTAAATAATAGTATTTTTACTAGAAGGGATGATAATATTTTATAAA

Intron 5 GTAAGATTAC ... TTGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	84.88	132	741	272
A2	82.02	130	593	268
A3	83.41	148	744	260
A4	82.2	110	508	208
A5	81.73	97	434	169
A6	80.07	4922	19771	6558
A7	82.18	2761	12730.5	3555
A8	81.86	3468	15646.5	5023
A9	81.46	2979	13090.5	3920
A10	82.9	1760	8530.5	2206

>TRINITY_DN42543_c0_g1_i5 from sample A10 / Example spliced isoform

CTTGGAAAAAACAGAAAGAGTGGAGGCGGGTTTGTTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGAC
TTCTGGTTCAGAGCCACGAAAATCAATTGCAATGGCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACG
TTACGGGCCTGAGCAACGACTCCAACCCAATATGCTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGGTGCTGAT
GTTCCGTACAAGCCGCCCACCACAGGTCTGCCAGTGACGCCTCCAAATGCTGTCCCAGGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGG
TCATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATG
GCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTCATCCAATGTTATTTAAATAATAGTATTTTTACTATAAGGGATGATAATATTTTATAAAATTAAATAATAGTATTTTTACTAGAAGGGATGATAATATTTTATAAAATTAAATAATAGTA
TTTTTACTAGAAGGGATGATAATATTTTATAAA

>TRINITY_DN42543_c0_g1_i3 from sample A10 / Example unspliced isoform:

CTTGGAAAAAACAGAAAGAGTGGAGGCGGGTTTGTTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGAC
TTCTGGTTCAGAGCCACGAAAATCAATTGCAATGGCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACG
TTACGGGCCTGAGCAACGACTCCAACCCAATATGCTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGGTTCGATT
CCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTT
TCTAGCCAGGACCTATTTCATgtaagattacacttagacgactgttttaatggcggacgcatgatcttgcttgcagtacttgtgttttcggatttttaagaatgtggacaaaggattttatatttctccgtaccagtttttcttttgtgcatggcaatgaagctggaaaacagagacggccttgaagagttatatatata
tgtttttggttgcagACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTT
AAAATTTTCATCCAATGTTATTTAAATAATAGTATTTTTACTATAAGGGATGATAATATTTTATAAAATTAAATAATAGTATTTTTACTAGAAGGGATGATAATATTTTATAAAATTAAATAATAGTATTTTTACTAGAAGGGATGATAATATTTTATAAA

Intron 6 GTACACAATT ... GATGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.33	460	99	181
A2	23.47	362	97	125
A3	24.56	467	149	155
A4	23.49	355	79	139
A5	23.76	361	90	135
A6	23.22	12052	3062	4227
A7	23.01	7932	1979	2762
A8	23.36	8491	2150	3026
A9	23.21	8762	2215	3083
A10	23.09	6265	1759	2002

>TRINITY_DN42218_c0_g1_i2 from sample A7 / Example spliced isoform

TGGAAAAAACAGAAAGAGTGGAGGCGGGTTTGTTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTT
CTGGTTCAGAGCCACGAAAATCAATTGCAATGGCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTT
ACGGGCCTGAGCAACGACTCCAACCCAATATGCTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGGTTCGATTCC
TGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGGGTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTC
TAGCCAGGACCTATTTCATGTAAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAGTACTTGTGTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGGCAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATG
TTTTTGGTTGCAGACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAATTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAA
AATTTTCATCCA

>TRINITY_DN42218_c0_g1_i3 from sample A7 / Example unspliced isoform:

TGGAAAAAACAGAAAGAGTGGAGGCGGGTTTGTTACAAACCACAGCACTTTCTAAAACTGTCAATATTCGCCTTCTTACATTCGTATGCGATGTATGCCTTTTATTCCTTGAAGTTGGTCACATTCTTTCTTTCCCACGCTTCCTAACCACTTCAAATAACACTCGGCTGCACTCCCGTTCATCCTCCGATCTTCGACTT
CTGGTTCAGAGCCACGAAAATCAATTGCAATGGCGGGCGTGGCAAGGCGGAGGCGCCTAGTCGCAGCAACATTGGCCGTGGCGGTGCTCCTCCTTGCGGCCGGAAACGCGGTCGAAGTCGCAGCTCAGATCAGTGCTCCTACAGCCCCGCCAGCATGCCCAAACTTCATCTTGGCTATGGCTCCGTGTCTTGGCTACGTT
ACGGGCCTGAGCAACGACTCCAACCCAATATGCTGTGCCGCCTTTGCTGTGGTCGCCAGAACTTCACCTGCCTGCCTGTGCCAGTTTTTCTCTGTACCGAGTCCCCTCGGCATTCCTATGAACCTGACGCGGGTTCGAAACCTACCGAGAGAGTGCAACATCAGCGTCTCGTCTCTCCCACAGTGCACAGgtacacaatt
aaaccctaatactagaaagcttttaacaatgccaaaggctttcgcgtgatctttttcttgtttactgttactaactgcaggtgctgatgttccgtacaagccgcccaccacaggtctgccagtgacgcctccaaatgctgtcccaggcaggatctcagggttttctttggtccattacccattctttaatttctttgtaa
attctgattttcttagctttgttgctctgatgtttcagGTTCGATTCCTGCGCCCCCAACAACACCGCCTGCGGGCTTCTTTCCTGCTACTCCTACCACGGGCTCTCCAATTCCGGCGCCGGGCACTACCTCCGCTCCCAGTATGCCGCCAATTTTTCCTCGCCTCCCCCATCCGTCGGGTCATAATACGTCGGGTGCGG
GTGCCCTGTCCAGACCGTCTGCCATGGGCGTTTGTTTAGGAGTTTTTCTAGCCAGGACCTATTTCATGTAAGATTACACTTAGACGACTGTTTTAATGGCGGACGCATGATCTTGCTTGCAGTACTTGTGTTTTCGGATTTTTAAGAATGTGGACAAAGGATTTTATATTTCTCCGTACCAGTTTTTCTTTTGTGCATGG
CAATGAAGCTGGAAAACAGAGACGGCCTTGAAGAGTTATATATATATGTTTTTGGTTGCAGACCAAGGGTTTTTTCTTTTAGCAGGGACAGGGCCGTGGCTTCTCCAATTTGGACGGCAGCTACAGGGTTTGTCCCTCGTGTTTTTGTTAATGCTGTTTAAGTTTACTGTGATGGCCATTTACTGTAAGGTTTTTAAAAA
TTTGTTTGCCGAAGATCATATTTCTACGATGGATAATAAACAATTTAAAATTTTCATCCA