sp|Q0JA29|FLS2_ORYSJ ----> LRR receptor-like serine/threonine-protein kinase FLS2

Intron 1 GTGAAATGGA ... TTTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.34	9	40	15
A2	70.59	5	20	4
A3	70.82	7	20	14
A4	82.22	2	10.5	8
A5	53.26	5	8.5	3
A6	65.56	8	17.5	13
A7	67.73	10	28	14
A8	76.16	9	44	13.5
A9	58.61	12	24	10
A10	73.33	8	27	17

>TRINITY_DN133738_c1_g1_i12 from sample A1 / Example spliced isoform

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGACCGTG
ACTCCCAGCGCAGATGTGTTCAGCTTTGGCCTAATTCTCACGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGAAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAA
GATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATAGTCCAAGCATGAGCGAAGTGCTGCCCTAGATGTTGCAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATTATCGTTTTATTCTACACTGGAATATAGTGATCTGGACTGAATTGACTCGTCTGACCGCTGAAAT
GGAAGTTTCTGCTTGGAAATTAGAAATTAACAGGGCCATATTTCCATCTACAATTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCCGGATGACATGATGTCTTGAAGCAACCTAGCTATCTCGATTCCTTGGGCACAAGAAAGTTCTCTCAGCCGTGTTAGCACCACGGTCTTGGTGGGGGAGC
CCTTCCGTAGTGGGATTACCAAATGCAGATTTATAAAGAAGACCGCATTGTATTCAAGATGC

>TRINITY_DN133738_c1_g1_i6 from sample A1 / Example unspliced isoform:

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGACCGTG
ACTCCCAGCGCAGATGTGTTCAGCTTTGGCCTAATTCTCACGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGAAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAA
GATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATAGTCCAAGCATGAGCGAAGTGCTGCCCTAGATGTTGCAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATGTAAGTAGTCTTTCAAAATTGTCTTATTCTTGCGTGGAATTTAGAAATAATGGATGGACTGGATG
CAATACTGTCTTCTTCTTTCTTGCAGTATCGTTTTATTCTACACTGGAATATAGTGATCTGGACTGAATTGACTCGTCTGACCGCTGAAATGGAAGTTTCTGCTTGGAAATTAGAAATTAACAGgtgaaatggaagggcatgattgagtctacaataacggcgaccctcttcgcttccgggcattttgcagtgccatatt
tccatctacgagagagaatggagcatttgatgggttttttttgcagtgccatattatctacgagagagaatggagcatctgatggtttttttttgcagGGCCATATTTCCATCTACAATTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCCGGATGACATGATGTCTTGAAGCAACC

Intron 2 GTAAGTAGTC ... TTTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.68	23	21	35.5
A2	55.56	8	1	10
A3	77.63	7.5	5	26
A4	75	4	9	12
A5	66.67	4	5	8
A6	71.25	10.5	3	26
A7	58.93	23	5	33
A8	70	15	15	35
A9	54.78	26	6	31.5
A10	58.81	28	2	40

>TRINITY_DN133738_c1_g1_i5 from sample A1 / Example spliced isoform

TCAATCTTAGCTTTTTTCAAAATATAGTTACAAAAGCCGTGCAGTCACACCCATGCATGCATACAGCCGATGATAACGGCATTCTTGCTTTCAAAATAGATGGATACCTTCTTAGTGGTGAGACGAGCACAGGTGTAGCTGAAGCATTGGTTTATTTGCACGAGGATTACGATTTCCCCATCGTCCACTGTGATCTGAAG
CCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGCGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGACCGTGACTCCCAGCGCAGATGTGTTCAGCTTTGGCCTA
ATTCTCACGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGAAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTG
TACCAAAGCAATAGTCCAAGCATGAGCGAAGTGCTGCCCTAGATGTTGCAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATGGCCATATTTCCATCTACAACTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCCGGATGACATGATGTCTTGAAGCAACCCAG
CTATCTCGATTCCTTAGATCTCTGACCAATTTATTTCACTGGAGAATAGATCTTGCCGAGATTTTTGGGCACAAGAAAGTTCTCTCAGCCGTGTTAGCACCAC

>TRINITY_DN133738_c1_g1_i9 from sample A1 / Example unspliced isoform:

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGACCGTG
ACTCCCAGCGCAGATGTGTTCAGCTTTGGCCTAATTCTCACGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGAAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAA
GATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATAGTCCAAGCATGAGCGAAGTGCTGCCCTAGATGTTGCAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATgtaagtagtctttcaaaattgtcttattcttgcgtggaatttagaaataatggatggactggatg
caatactgtcttcttctttcttgcagtatcgttttattctacactggaatatagtgatctggagtggattgactcgtctgaccgctgaaatggaagtttctgcttggaaattagaaattaacgggtgaaatggaagggcatgattgagtctacaataacggcgaccctcttcgcttccgggcattttgcagtgccatatt
tccatctacgagagagaatggagcatctgatgggttttttttgcagGGCCATATTTCCATCTACAATTCTCCTCAATATATTGAATGCACTTTTTCCTCGCCTTCTGACCATTCCAGATGACATGATGTCTTGAAGCAACCCAGCTATCTCGATTCCTTAGATCTCTGACCAATTTATTTCACTGGAGAATAGATCTTGC
CGAGATTTTTGGGCACAAGAAAGTTCTCTCAGCCGTGTTAGCACCACGGTCTTGGTGGGGGAGCCCTTCCGTAGTGGGATTACTGCATCGAATCATATGGCCACATTGGGATGCCAAATGCAGATTTATAAAGAAGACCGCATTGTATTCAAGATGC

Intron 3 GCAAGAATAA ... GCAATTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.81	46	7	9
A2	16.17	23.3	3	6
A3	10.86	53.3	3	10
A4	33.33	14	7	7
A5	9.21	24.7	5	0
A6	23.33	23	6	8
A7	20.21	51.3	9	17
A8	13.98	40	2	11
A9	9.24	54	5	6
A10	9.84	55	7	5

>TRINITY_DN133738_c1_g1_i13 from sample A1 / Example spliced isoform

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGGTGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGCGACTTTGGTACTGCCAGGATTCTTGGGATCCATCTCCAGCCAGAACTAAGCCAGGGGACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGAC
CGTGACTCCCAGCGCAGATGTGTTCAGCTTTGGCATAATTCTCATGGAGTTATCGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGCAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTC
TGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCCAAGCATGAGCCAAGCGCTGCCCTAGATGTTGGAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATGTAAGTAGTCTTTCAAAATTGTCTTATTCTTGCGTGGAATTTAGAAATAAT
GGATGGACTGGATGCAATACTGTCTTCTTCTTTCTTGCAGTATCGTTTTATTCTACACTGGAATATAGTGATCTGGACTGAATTGACTCGTCTGACCGCTGAAATGGAAGTTTCTGCTTGGAAATTAGAAATTAACAGG

>TRINITY_DN133738_c1_g1_i16 from sample A1 / Example unspliced isoform:

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGgcaagaataatacattacataac
ttaaccatttattaggtttgtttatatcatgacatcaagtatttaaattaagcgaccatcactgttaacaaggtgtctggtgcaattacagAGTTTGCATATATGGCGACCGTGACTCCCAGCGCAGATGTGTTCAGCTTTGGCATAATTCTCATGGAGTTATCGACAAGGACAAGACCCACCAATATTGCTAGCTCGTC
TTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGCAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCCAAGCATGAGCCAAGCGCTGCCCTAGA
TGTTGGAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATTATCGTTTTATTCTACACTGGAATATAGTGATCTGGACTGAATTGACTCGTCTGACCGCTGAAATGGAAGTTTCTGCTTGGAAATTAGAAATTAACAGGTGAAATGGAAGGGCATGATTGAGTCTACAATAACGGCGACCC
TCTTCGCTTCCGGGCATTTTGCAGTGCCATATTTCCATCTACGAGAGAGAATGGAGCATTTGATGGGTTTTTTTTGCAGTGCCATATTATCTACGAGAGAGAATGGAGCATCTGATGGTTTTTTTTTGCAGGGCCATATTTCCATCTACAATTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCC
GGATGACATGATGTCTTGAAGCAACC

Intron 4 GTAAGTAGTC ... CATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.76	8	21	29
A2	65.71	6	5	18
A3	90.48	1	5	14
A4	85.71	1	8	4
A5	80	1	5	3
A6	65.52	5	4	15
A7	73.91	6	4	30
A8	65.43	14	18	35
A9	70.73	6	6	23
A10	74.47	6	5	30

>TRINITY_DN48527_c2_g1_i8 from sample A7 / Example spliced isoform

CTTGGGATCCATCTCCAGCCAGAACTAAGCCAGGGGACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGACCGTGACTCCCAGCGCAGATGTGTTCAGCTTTGGCCTAATTCTCATGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTC
TGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGCAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGCCTGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCCAAGCATGAGCGAAGTGCTGCCCTAGATGTTGCAGG
TCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATTGCCATATTTCCATCTACGAGAGAGAATGGAGCATCTGATGG

>TRINITY_DN48527_c2_g1_i12 from sample A7 / Example unspliced isoform:

CTTTATCCATAAAGCAAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTT
ATTTGCACGAGGATTACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTTTGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCAT
ATATGGCGACCGTGACTCCCAGCGCAGATGTGTTCAGCTTTGGCCTAATTCTCATGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGCAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTAC
AGAATGTGCCTGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCCAAGCATGAGCCAAGCGCTGCCCTAGATGTTGGAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATgtaagtagtctttcaatattgtcttattcttgcttggaatt
tagaaataatggatggactggatgcaatactgtcttcttctttcttgcagtatcgttttattctacactggaatatagtgatctggactgaattgactcgtctgaccgctgaaatggaagtttctgcttggaaattagaaattaacgggtgaaatggaagggcatgattgagtctacaataacggcgaccctcttcgctt
ccgggcattttgcagTGCCATATTTCCATCTACGAGAGAGAATGGAGCATTTGATGGGTTTTTTTTGCAGTGCCATATTATCTACGAGAGAGAATGGAGCATCTGATGGTTTTTTTTTGCAGGGCCATATTTCCATCTACAATTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCCGGATGAGAT
GATGTCTTGAAGCAACCTAGCTATCTCGATTCCTTGG

Intron 5 GTGAGACGAG ... CCTGTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.77	27	121	39
A2	79.83	12	66	29
A3	81.46	14	95	28
A4	73.96	22	82	43
A5	78.22	11	63	16
A6	81.68	12	82	25
A7	83.26	19	133	56
A8	72.15	22	75	39
A9	86.14	14	123	51
A10	78.52	29	157	55

>TRINITY_DN48527_c2_g1_i7 from sample A7 / Example spliced isoform

CTTTATCCATAAAGCAAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGAATGTGCCTGAAGATGGCAAGTTTATTGAAGATCTC
GTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCTAAGCATGAGCCAAGTGCTGCCCTAGATGTTGCAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATTATCGTTTTATTCTACACTGGAATATAGTGATCTGGAGTGGATTGACTCGTCTGACCGCTGAAATGGAAGTTTCTGCTT
GGAAATTAGAAATTAACGGGTGAAATGGAAGGGCATGATTGAGTCTACAATAACGGCGACCCTCTTCGCTTCCGGGCATTTTGCAGTGCCATATTTCCATCTACGAGAGAGAATGGAGCAT

>TRINITY_DN48527_c2_g1_i12 from sample A7 / Example unspliced isoform:

CTTTATCCATAAAGCAAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGgtgagacgagcacagatgtagctgaagcattggttt
atttgcacgaggattacgatttccccatcgtccactgtgatctgaagcctgcaaacattcttctggacgaagaatttgaagctcatctgagagactttggtactgccaggatgcttgggatccatctccagccagactttggtacacgttgtctgcttttcagctggggaccatcggctacgttgcaccagagtttgcat
atatggcgaccgtgactcccagcgcagatgtgttcagctttggcctaattctcatggagttattgacaaggacaagacccaccaatattgctagctcgtcttctgcttctgaccaaagaagcacagtttacaagaatggatagaaaatggtttcaaggattgcaagaacttaattagtccgattgatcctctcctgttac
agAATGTGCCTGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCCAAGCATGAGCCAAGCGCTGCCCTAGATGTTGGAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATGTAAGTAGTCTTTCAATATTGTCTTATTCTTGCTTGGAATT
TAGAAATAATGGATGGACTGGATGCAATACTGTCTTCTTCTTTCTTGCAGTATCGTTTTATTCTACACTGGAATATAGTGATCTGGACTGAATTGACTCGTCTGACCGCTGAAATGGAAGTTTCTGCTTGGAAATTAGAAATTAACGGGTGAAATGGAAGGGCATGATTGAGTCTACAATAACGGCGACCCTCTTCGCTT
CCGGGCATTTTGCAGTGCCATATTTCCATCTACGAGAGAGAATGGAGCATTTGATGGGTTTTTTTTGCAGTGCCATATTATCTACGAGAGAGAATGGAGCATCTGATGGTTTTTTTTTGCAGGGCCATATTTCCATCTACAATTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCCGGATGAGAT
GATGTCTTGAAGCAACCTAGCTATCTCGATTCCTTGG

Intron 6 GTAAGTAGTC ... TTGCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.41	30.8	19.8	33.1
A2	42.59	13.2	2.4	9.7
A3	31.73	16	4.4	6.8
A4	37.01	12.2	8.3	6.9
A5	45.54	8.3	4.8	6.8
A6	18.77	17	2.8	3.8
A7	33.5	13.7	3.9	6.8
A8	37.48	35.5	13.3	21.1
A9	13.59	22.1	4.7	3.5
A10	33.41	13.9	2.5	6.7

>TRINITY_DN133738_c1_g1_i16 from sample A1 / Example spliced isoform

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGGCAAGAATAATACATTACATAAC
TTAACCATTTATTAGGTTTGTTTATATCATGACATCAAGTATTTAAATTAAGCGACCATCACTGTTAACAAGGTGTCTGGTGCAATTACAGAGTTTGCATATATGGCGACCGTGACTCCCAGCGCAGATGTGTTCAGCTTTGGCATAATTCTCATGGAGTTATCGACAAGGACAAGACCCACCAATATTGCTAGCTCGTC
TTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGCAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCCAAGCATGAGCCAAGCGCTGCCCTAGA
TGTTGGAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATTATCGTTTTATTCTACACTGGAATATAGTGATCTGGACTGAATTGACTCGTCTGACCGCTGAAATGGAAGTTTCTGCTTGGAAATTAGAAATTAACAGGTGAAATGGAAGGGCATGATTGAGTCTACAATAACGGCGACCC
TCTTCGCTTCCGGGCATTTTGCAGTGCCATATTTCCATCTACGAGAGAGAATGGAGCATTTGATGGGTTTTTTTTGCAGTGCCATATTATCTACGAGAGAGAATGGAGCATCTGATGGTTTTTTTTTGCAGGGCCATATTTCCATCTACAATTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCC
GGATGACATGATGTCTTGAAGCAACC

>TRINITY_DN133738_c1_g1_i13 from sample A1 / Example unspliced isoform:

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGGTGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGCGACTTTGGTACTGCCAGGATTCTTGGGATCCATCTCCAGCCAGAACTAAGCCAGGGGACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGAC
CGTGACTCCCAGCGCAGATGTGTTCAGCTTTGGCATAATTCTCATGGAGTTATCGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGCAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTC
TGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCCAAGCATGAGCCAAGCGCTGCCCTAGATGTTGGAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATgtaagtagtctttcaaaattgtcttattcttgcgtggaatttagaaataat
ggatggactggatgcaatactgtcttcttctttcttgcagTATCGTTTTATTCTACACTGGAATATAGTGATCTGGACTGAATTGACTCGTCTGACCGCTGAAATGGAAGTTTCTGCTTGGAAATTAGAAATTAACAGG

Intron 7 GCAAGAATAA ... TGACTCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.56	29	7	25
A2	48.15	7	3	10
A3	56.25	7	3	15
A4	48.94	12	7	16
A5	45.45	3	5	0
A6	46.34	11	6	13
A7	45.71	19	9	23
A8	60	6	2	16
A9	38.03	22	5	22
A10	34.33	22	7	16

>TRINITY_DN133738_c1_g1_i10 from sample A1 / Example spliced isoform

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGCGCAGATGTGTTCAGCTTTGGCA
TAATTCTCACGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTG

>TRINITY_DN133738_c1_g1_i11 from sample A1 / Example unspliced isoform:

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGgcaagaataatacattacataac
ttaaccatttattaggtttgtttatatcatgacatcaagtatttaaattaagcgaccatcactgttaacaaggtgtctggtgcaattacagagtttgcatatatggcgaccgtgactcccagCGCAGATGTGTTCAGCTTTGGCATAATTCTCATGGAGTTATCGACAAGGACAAGACCCACCAATATTGCTAGCTCGTC
TTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGCAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATACCAGAGAATCGTCCAAGCATGAGCCAAGCGCTGCCCTAGA
TGTTGGAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATGTAAGTAGTCTTTCAAAATTGTCTTATTCTTGCGTGGAATTTAGAAATAATGGATGGACTGGATGCAATACTGTCTTCTTCTTTCTTGCAGTATCGTTTTATTCTACTTAGGAATATAGTGATCTGGAGTGGATTGACTCG
TCTGACCGCTGAAATGGAAGTTTCTGCTTGGAAATTAGAAATTAACAGGTGAAATGGAAGGGCATGATTGAGTCTACAATAACGGCGACCCTCTTCGCTTGATGGGTTTTTTTGCAGGAAACCATCTACAATTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCCGGATGACATGATGTCTTGAA
GCAACC

Intron 8 GTAAGTAGTC ... TTTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.28	16.5	21	17
A2	31.91	8.5	3	5.5
A3	71.1	4.5	5	15.5
A4	79	2.5	8.5	9
A5	60.72	3	5	4
A6	52.86	8	3.5	14
A7	41.36	16.5	4.5	16.5
A8	54.58	13	16.5	14.5
A9	35.35	20	6	14.5
A10	32.97	23.5	3.5	19

>TRINITY_DN133738_c1_g1_i5 from sample A1 / Example spliced isoform

TCAATCTTAGCTTTTTTCAAAATATAGTTACAAAAGCCGTGCAGTCACACCCATGCATGCATACAGCCGATGATAACGGCATTCTTGCTTTCAAAATAGATGGATACCTTCTTAGTGGTGAGACGAGCACAGGTGTAGCTGAAGCATTGGTTTATTTGCACGAGGATTACGATTTCCCCATCGTCCACTGTGATCTGAAG
CCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGCGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGACCGTGACTCCCAGCGCAGATGTGTTCAGCTTTGGCCTA
ATTCTCACGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGAAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAAGATGGCAAGTTTATTGAAGATCTCGTTGCTGTG
TACCAAAGCAATAGTCCAAGCATGAGCGAAGTGCTGCCCTAGATGTTGCAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATGGCCATATTTCCATCTACAACTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCCGGATGACATGATGTCTTGAAGCAACCCAG
CTATCTCGATTCCTTAGATCTCTGACCAATTTATTTCACTGGAGAATAGATCTTGCCGAGATTTTTGGGCACAAGAAAGTTCTCTCAGCCGTGTTAGCACCAC

>TRINITY_DN133738_c1_g1_i6 from sample A1 / Example unspliced isoform:

CCAATGAGATGGTTGAATCCGAGGAAATCGTTTGGAATATTGGCGAGTATGTAGTTGCAAAGTTCGAGCGTGGTGAAACGAGCGGGAGGGGGGATTTAGCAGAGATGGACATTGTTGGATGCTGCATATTCAACCCATACCTTCCCAGTGGTGAGACGAGCACAGATGTAGCTGAAGCATTGGTTTATTTGCACGAGGAT
TACGATTTCCCCATCGTCCACTGTGATCTGAAGCCTGCAAACATTCTTCTGGACGAAGAATTTGAAGCTCATCTGAGAGACTATGGTACTGCCAGGATGCTTGGGATCCATCTCCAGCCAGACTTTGGTACACGTTGTCTGCTTTTCAGCTGGGGACCATCGGCTACGTTGCACCAGAGTTTGCATATATGGCGACCGTG
ACTCCCAGCGCAGATGTGTTCAGCTTTGGCCTAATTCTCACGGAGTTATTGACAAGGACAAGACCCACCAATATTGCTAGCTCGTCTTCTGCTTCTGACCAAAGAAGCACAGTTTACAAGAATGGATAGAAAATGGTTTCAAGGATTGAAAGAACTTAATTAGTCCGATTGATCCTCTCCTGTTACAGAATGTGTCTGAA
GATGGCAAGTTTATTGAAGATCTCGTTGCTGTGTACCAAAGCAATAGTCCAAGCATGAGCGAAGTGCTGCCCTAGATGTTGCAGGTCAAAGAGAACCGCATGCATGCATGATGATAAACATTGAGATTTCTGGATgtaagtagtctttcaaaattgtcttattcttgcgtggaatttagaaataatggatggactggatg
caatactgtcttcttctttcttgcagtatcgttttattctacactggaatatagtgatctggactgaattgactcgtctgaccgctgaaatggaagtttctgcttggaaattagaaattaacaggtgaaatggaagggcatgattgagtctacaataacggcgaccctcttcgcttccgggcattttgcagtgccatatt
tccatctacgagagagaatggagcatttgatgggttttttttgcagtgccatattatctacgagagagaatggagcatctgatggtttttttttgcagGGCCATATTTCCATCTACAATTCTCCTCAATATATTGAATGCACTTTCTCCTCGCCTTCTGACCATTCCGGATGACATGATGTCTTGAAGCAACC