sp|P31583|RHN1_NICPL ----> Ras-related protein RHN1

Intron 1 GTTAATTACA ... ATGTTTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.09	89	21	21
A2	18.25	103	17	29
A3	18.15	124	33	22
A4	20.39	82	26	16
A5	15	68	15	9
A6	15.92	462	74	101
A7	16.02	456	79	95
A8	16.27	350	59	77
A9	15.12	466	84	82
A10	14.02	325	54	52

>TRINITY_DN106215_c2_g1_i9 from sample A4 / Example spliced isoform

TTATTGGCGAGCCAAGGGAAATTCGCTCGCAAATCATTGTTTGGTCTGCTGTTCGATTCGCAGCGATCACCAAATTCTGAGCTACAGATCTTCATTCTCACTCCTCCAAAATCTGCGGGCAATCTGCAGAGAAAAATTCGAAGAATTTGAGTGTCAGCATCGAATCCCCTGATTGAATGGCTACCACGGGCACCAATAAC
ATGCAGGCCAAGCTGGTGCTTCTTGGTGATATGGGTACTGGAAAATCGAGCCTCGTTTTGCGATTTGTCAAAGGCCAATTTTTGGAATATCAGGAGTCGACAATTGGAGCAGCATTTTTCTCACAGACACTAGCTGTGAATGAGGTCACTGTAAAATTTGAGATATGGGACACTGCAGGACAAGAAAGATATCATAGCTT
GGCTCCCATGTATTATCGTGGTGCTGCTGCAGCGATAATTGTTTATGACATCACAAATTCGGATTCTTTTACCCGAGCAAAGAATTGGGTGCTGGAACTTCAAAAACAAGGCAACCCAAATTTGGTTATGGCCCTTGCTGGAAACAAGGCTGATGCGGCAGCGAAGAGGAAGGTTGAAGCAGAGGAAGCTGAAACATATG
CAAAGGAAAATGGTCTATTTTTCATGGAAACCTCAGCAAAGACGGCGCAAAATGTCAATGAACTGTTCTATGAAATTGCACGGAGATTACCTAAAGCACGACCGGTGCAGCAGCCTGCAGGCATGGTTCTCACAGATAGACCTGCAGAGGGAGCTAGAACCTACTCTTGCTGCTCATAAGCAGCAAGCAGTTGTTCTATG
CACATTATGTGCTACAGCTGTCATATCCGGAGGAGATGGTGGAGGGTTCAAGCTTTGTTTAGAACTCGATTTAAGGAATATTAGCAGTTTATGGAGAAATGTAAAAATACATATTTTGGTGTTTAAATTAAATCTTTGTACTGAGTTACGATGGTCATTGAGAGTCTTCTAATACAGCTCTTTGTGTCATTTGTGATGAG
CTTATATTTAAATTGAAAGCTATGCTCTGTTTAATTACTTCGATCTCGGAGAAGAAAATCCCTTTTGATCCTACATTGACATCTTGTGTTTGATTGTACTAGATTGTACTATGAGACGTCTAGCCTGTCATTTAAAAATATAAGTCCGTTG

>TRINITY_DN106215_c2_g1_i5 from sample A4 / Example unspliced isoform:

TTATTGGCGAGCCAAGGGAAATTCGCTCGCAAATCATTGTTTGGTCTGCTGTTCGATTCGCAGCGATCACCAAATTCTGAGCTACAGATCTTCATTCTCACTCCTCCAAAATCTGCGGGCAATCTGCAGAGAAAAATTCGAAGAATTTGAGTGTCAGCATCGAATCCCCTGATTGAATGGCTACCACGGGCACCAATAAC
ATGCAGGCCAAGCTGGTGCTTCTTGGTGATATGGGTACTGGAAAATCGAGCCTCGTTTTGCGATTTGTCAAAGGCCAATTTTTGGAATATCAGgttaattacacccttgctgttgttttctcttcaaggctttgtttgattattggttcaattgcttgatttaaggtgtcctacatgtttttagGAGTCGACAATTGGAG
CAGCATTTTTCTCACAGACACTAGCTGTGAATGAGGTCACTGTAAAATTTGAGATATGGGACACTGCAGGACAAGAAAGATATCATAGCTTGGCTCCCATGTATTATCGTGGTGCTGCTGCAGCGATAATTGTTTATGACATCACAAATTCGGATTCTTTTACCCGAGCAAAGAATTGGGTGCTGGAACTTCAAAAACAA
GGCAACCCAAATTTGGTTATGGCCCTTGCTGGAAACAAGGCTGATGCGGCAGCGAAGAGGAAGGTTGAAGCAGAGGAAGCTGAAACATATGCAAAGGAAAATGGTCTATTTTTCATGGAAACCTCAGCAAAGACGGCGCAAAATGTCAATGAACTGTTCTATGAAATTGCACGGAGATTACCTAAAGCACGACCGGTGCA
GCAGCCTGCAGGCATGGTTCTCACAGATAGACCTGCAGAGGGAGCTAGAACCTACTCTTGCTGCTCATAAGCAGCAAGCAGTTGTTCTATGCACATTATGTGCTACAGCTGTCATATCCGGAGGAGATGGTGGAGGGTTCAAGCTTTGTTTAGAACTCGATTTAAGGAATATTAGCAGTTTATGGAGAAATGTAAAAATA
CATATTTTGGTGTTTAAATTAAATCTTTGTACTGAGTTACGATGGTCATTGAGAGTCTTCTAATACAGCTCTTTGTGTCATTTGTGATGAGCTTATATTTAAATTGAAAGCTATGCTCTGTTTAATTACTTCGATCTCGGAGAAGAAAATCCCTTTTGATCCTACATTGACATCTTGTGTTTGATTGTACTAGATTGTAC
TATGAGACGTCTAGCCTGTCATTTAAAAATATAAGTCCGTTG

Intron 2 GTGCTTCTTG ... TGAAGCAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.81	64	108	75
A2	54.18	72	88	82.5
A3	56.45	72	105	82
A4	57.63	60	77	86.5
A5	50.82	50	48	55.5
A6	50.72	455	324	618.5
A7	49.63	438	319	550
A8	50.14	353	263	451
A9	51.51	399	362	489
A10	48.66	356	273	405

>TRINITY_DN106215_c2_g1_i3 from sample A4 / Example spliced isoform

TTATTGGCGAGCCAAGGGAAATTCGCTCGCAAATCATTGTTTGGTCTGCTGTTCGATTCGCAGCGATCACCAAATTCTGAGCTACAGATCTTCATTCTCACTCCTCCAAAATCTGCGGGCAATCTGCAGAGAAAAATTCGAAGAATTTGAGTGTCAGCATCGAATCCCCTGATTGAATGGCTACCACGGGCACCAATAAC
ATGCAGGCCAAGCTGGAAGCTGAAACATATGCAAAGGAAAATGGTCTATTTTTCATGGAAACCTCAGCAAAGACGGCGCAAAATGTCAATGAACTGTTCTATGAAATTGCACGGAGATTACCTAAAGCACGACCGGTGCAGCAGCCTGCAGGCATGGTTCTCACAGATAGACCTGCAGAGGGAGCTAGAACCTACTCTTG
CTGCTCATAAGCCCTATATTTCTTGTTACTGAAAATTTGAAGATGCCACTTTTCAGCAGCAAGCAGTTGTTCTATGCACATTATGTGCTACAGCTGTCATATCCGGAGGAGATGGTGGAGGGTTCAAGCTTTGTTTAGAACTCGATTTAAGGAATATTAGCAGTTTATGGAGAAATGTAAAAATACATATTTTGGTGTTT
AAATTAAATCTTTGTACTGAGTTACGATGGTCATTGAGAGTCTTCTAATACAGCTCTTTGTGTCATTTGTGATGAGCTTATATTTAAATTGAAAGCTATGCTCTGTTTAATTACTTCGATCTCGGAGAAGAAAATCCCTTTTGATCCTACATTGACATCTTGTGTTTGATTGTACTAGATTGTACTATGAGACGTCTAGC
CTGTCATTTAAAAATATAAGTCCGTTG

>TRINITY_DN106215_c2_g1_i4 from sample A4 / Example unspliced isoform:

TTATTGGCGAGCCAAGGGAAATTCGCTCGCAAATCATTGTTTGGTCTGCTGTTCGATTCGCAGCGATCACCAAATTCTGAGCTACAGATCTTCATTCTCACTCCTCCAAAATCTGCGGGCAATCTGCAGAGAAAAATTCGAAGAATTTGAGTGTCAGCATCGAATCCCCTGATTGAATGGCTACCACGGGCACCAATAAC
ATGCAGGCCAAGCTGgtgcttcttggtgatatgggtactggaaaatcgagcctcgttttgcgatttgtcaaaggccaatttttggaatatcaggagtcgacaattggagcagcatttttctcacagacactagctgtgaatgaggtcactgtaaaatttgagatatgggacactgcaggacaagaaagatatcatagctt
ggctcccatgtattatcgtggtgctgctgcagcgataattgtttatgacatcacaaattcggattcttttacccgagcaaagaattgggtgctggaacttcaaaaacaaggcaacccaaatttggttatggcccttgctggaaacaaggctgatgcggcagcgaagaggaaggttgaagcagagGAAGCTGAAACATATG
CAAAGGAAAATGGTCTATTTTTCATGGAAACCTCAGCAAAGACGGCGCAAAATGTCAATGAACTGTTCTATGAAATTGGTAATGATTATAATGTGAAAGTTTCAGAGCTTGCATGCATGCTAATATTGTCATAAGCCAAACCTGTATCCTATTTGTGTGCCCTGCC

Intron 3 GTATAACCAG ... TTGGCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.97	83.3	4	20
A2	12.65	79.6	2	13.5
A3	7.37	73.3	0	7
A4	19.02	74.4	3	19.5
A5	12.5	36.8	0	7
A6	10.63	415.9	2	63
A7	10.04	369	0	52.5
A8	10.61	308.9	0	47.5
A9	8.14	429.5	0	46.5
A10	9.59	243.5	0.5	32.5

>TRINITY_DN118160_c1_g6_i7 from sample A1 / Example spliced isoform

GAGTTTCTCTTATTGGCGAGCCAAGGGAAATTCGCTCGCAAATCATTGTTTGGTCTGCTGTTCGATTCGCAGCGATCACCAAATTCTGAGCTACAGATCTTCATTCTCACTCCTCCAAAATCTGCGGGCAATCTGCAGAGAAAAATTCGAAGAATTTGAGTGTCAGCATCGAATCCCCTGATTGAATGGCTACCACGGGC
ACCAATAACATGCAGGCCAAGCTGGTGCTTCTTGGTGATATGGGTACTGGAAAATCGAGCCTCGTTTTGCGATTTGTCAAAGGCCAATTTTTGGAATATCAGGAGTCGACAATTGGAGCAGCATTTTTCTCACAGACACTAGCTGTGAATGAGGTCACTGTAAAATTTGAGATATGGGACACTGCAGGACAAGAAAGATA
TCATAGCTTGGCTCCCATGTATTATCGTGGTGCTGCTGCAGCGATAATTGTTTATGACATCACAAATTCGGATTCTTTTACCCGAGCAAAGAATTGGGTGCTGGAACTTCAAAAACAAGGCAACCCAAATTTGGTTATGGCCCTTGCTGGAAACAAGGCTGATGCGGCAGCGAAGAGGAAGGTTGAAGCAGAGGAAGCTG
AAACATATGCAAAGGAAAATGGTCTATTTTTCATGGAAACCTCAGCAAAGACGGCGCAAAATGTCAATGAACTGTTCTATGAAATTGCACGGAGATTACCTAAAGCACGACCGGTGCAGCAGCCTGCAGGCATGGTTCTCACAGATAGACCTGCAGAGGGAGCTAGAACCTACTCTTGCTGCTCATAAGCAGCAAGCAGT
TGTTCTATGCACATTATGTGCTACAGCTGTCATATCCGGAGGAGATGGTGGAGGGTTCAAGCTTTGTTTAGAACTCGATTTAAGGAATATTAGCAGTTTATGGAGAAATGTAAAAATACATATTTTGGTGTTTAAATTAAATCTTTGTACTGAGTTACGATGGTCATTGAGAGTCTTCTAATACAGCTCTTTGTGTCATT
TGTGATGAGCTTATATTTAAATTGAAAGCTATGCTCTGTTTAATTACTTCGATCTCGGAGAAGAAAATCCCTTTTGATCCTACATTGACATCTTGTGTTTGATTGTACTAGATTGTACTATGAGACGTCTAGCCTGTCATTTAAAAATATAAGTCCGTTAGT

>TRINITY_DN118160_c1_g6_i1 from sample A1 / Example unspliced isoform:

GGAACTTCAAAAACAAGgtataaccagattatatgtttggaatgactcacttttgactggcggaagttaaagagagactgatttgaacctggtaatctgggaaatatatttccctactgaattttgagccttggctgcagGCAACCCAAATTTGGTTATGGCCCTTGCTGGAAACAAGGCTGATGCGGCAGCGAAGAGGA
AGGTTGAAGCAGAGGTATTCTTATTCCTAATTATTATTATTTTTCTTTGAAGATAATGATACAGTTTTCAAATCAAAAAAATTACATATTATTAATGGTTACTGAAATGTATGTTTCCATAGTTTATGTTGATAATAAAATCTAAAAAAGTCCTTATGGGATTTTTGAAGGTAAGAATGATATTTTGTCCAATATGATGT
TTTATTACTTCTAAGCATAGTTACCGGATTCGGCCCTAGGCCTAACCGCACCCGAATCAGATTCTTCCGAATCGGATCCTCGGATTCTTCCACCTCATTTTGACAAACCTAAAGCTGTTTATGTA

Intron 4 GTTTCTTAGA ... AGGACCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.44	102	33	33
A2	22.31	94	25	29
A3	23.29	84	22	29
A4	21.5	84	22	24
A5	21.28	37	9	11
A6	20.27	464	114	122
A7	21.07	414	110	111
A8	22.16	367	117	92
A9	19.05	527	135	113
A10	21.96	311	100	75

>TRINITY_DN109089_c1_g1_i2 from sample A2 / Example spliced isoform

CTTATTGGCGAGCCAAGGGAAATTCGCTCGCAAATCATTGTTTGGTCTGCTGTTCGATTCGCAGCGATCACCAAATTCTGAGCTACAGATCTTCATTCTCACTCCTCCAAAATCTGCGGGCAATCTGCAGAGAAAAATTCGAAGAATTTGAGTGTCAGCATCGAATCCCCTGATTGAATGGCTACCACGGGCACCAATAA
CATGCAGGCCAAGCTGGTGCTTCTTGGTGATATGGGTACTGGAAAATCGAGCCTCGTTTTGCGATTTGTCAAAGGCCAATTTTTGGAATATCAGGAGTCGACAATTGGAGCAGCATTTTTCTCACAGACACTAGCTGTGAATGAGGTCACTGTAAAATTTGAGATATGGGACACTGCAGGACAAGAAAGATATCATAGCT
TGGCTCCCATGTATTATCGTGGTGCTGCTGCAGCGATAATTGTTTATGACATCACAAATTCGGATTCTTTTACCCGAGCAAAGAATTGGGTGCTGGAACTTCAAAAACAAGGCAACCCAAATTTGGTTATGGCCCTTGCTGGAAACAAGGCTGATGCGGCAGCGAAGAGGAAGGTTGAAGCAGAGCACGGAGATTACCTA
AAGCACGACCGGTGCAGCAGCCTGCAGGCATGGTTCTCACAGATAGACCTGCAGAGGGAGCTAGAACCTACTCTTGCTGCTCATAAGCAGCAAGCAGTTGTTCTATGCACATTATGTGCTACAGCTGTCATATCCGGAGGAGATGGTGGAGGGTTCAAGCTTTGTTTAGAACTCGATTTAAGGAATATTAGCAGTTTATG
GAGAAATGTAAAAATACATATTTTGGTGTTTAAATTAAATCTTTGTACTGAGTTACGATGGTCATTGAGAGTCTTCTAATACAGCTCTTTGTGTCATTTGTGATGAGCTTATATTTAAATTGAAAGCTATGCTCTGTTTAATTACTTCGATCTCGGAGAAGAAAATCCCTTTTGATCCTACATTGACATCTTGTGTTTGA
TTGTACTAGATTGTACTATGAGACGTCTAGCCTGTCATTTAAAAATATAAGTCCGTA

>TRINITY_DN109089_c1_g1_i9 from sample A2 / Example unspliced isoform:

CTTATTGGCGAGCCAAGGGAAATTCGCTCGCAAATCATTGTTTGGTCTGCTGTTCGATTCGCAGCGATCACCAAATTCTGAGCTACAGATCTTCATTCTCACTCCTCCAAAATCTGCGGGCAATCTGCAGAGAAAAATTCGAAGAATTTGAGTGTCAGCATCGAATCCCCTGATTGAATGGCTACCACGGGCACCAATAA
CATGCAGGCCAAGCTGGTGCTTCTTGGTGATATGGGTACTGGAAAATCGAGCCTCGTTTTGCGATTTGTCAAAGGCCAATTTTTGGAATATCAGGAGTCGACAATTGGAGCAGCATTTTTCTCACAGACACTAGCTGTGAATGAGGTCACTGTAAAATTTGAGATATGGGACACTGCAGGACAAGAAAGATATCATAGCT
TGGCTCCCATGTATTATCGTGGTGCTGCTGCAGCGATAATTGTTTATGACATCACAAATTCGgtttcttagatttatagctatgtttcatggggaattcttgtgccagtttgggtgtacccaagatggggatatccctttttctggttacccacccatggggtacaggaccacagGATTCTTTTACCCGAGCAAAGAATT
GGGTGCTGGAACTTCAAAAACAAGGCAACCCAAATTTGGTTATGGCCCTTGCTGGAAACAAGGCTGATGCGGCAGCGAAGAGGAAGGTTGAAGCAGAGGAAGCTGAAACATATGCAAAGGAAAATGGTCTATTTTTCATGGAAACCTCAGCAAAGACGGCGCAAAATGTCAATGAACTGTTCTATGAAATTGCACGGAGA
TTACCTAAAGCACGACCGGTGCAGCAGCCTGCAGGCATGGTTCTCACAGATAGACCTGCAGAGGGAGCTAGAACCTACTCTTGCTGCTCATAAGCAGCAAGCAGTTGTTCTATGCACATTATGTGCTACAGCTGTCATATCCGGAGGAGATGGTGGAGGGTTCAAGCTTTGTTTAGAACTCGATTTAAGGAATATTAGCA
GTTTATGGAGAAATGTAAAAATACATATTTTGGTGTTTAAATTAAATCTTTGTACTGAGTTACGATGGTCATTGAGAGTCTTCTAATACAGCTCTTTGTGTCATTTGTGATGAGCTTATATTTAAATTGAAAGCTATGCTCTGTTTAATTACTTCGATCTCGGAGAAGAAAATCCCTTTTGATCCTACATTGACATCTTG
TGTTTGATTGTACTAGATTGTACTATGAGACGTCTAGCCTGTCATTTAAAAATATAAGTCCGTA

Intron 5 GTTATATTCT ... CTTCTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.61	37	2	35
A2	42.22	26	2	19
A3	34.21	50	12	26
A4	39.62	32	4	21
A5	30.42	18.3	2	8
A6	53.8	79	9	92
A7	38.79	172	8	109
A8	36.71	189.7	21	110
A9	42.09	139	13	101
A10	33.43	157.3	23	79

>TRINITY_DN109691_c1_g1_i11 from sample A3 / Example spliced isoform

ATTTTTGTGTTTTTGTTGGGTTCAGTGCGTGGAAACTAAGGCAAGCCCAAACAGTCCAGGTGATGTAGATTCCACAAGTGTGGTGGGTGCAAGTGAAGGCTAATGAGAAGGTACAACCGTGGCCTCTTCTTTATTGGACAAGCAAGGAAGAAAGAGCTTGCTTACAGCCAGTTTTACGGGAATGGTGTTTCTTGGTGATA
TGGGTTCTGGAAAATCGAGCCTCATTTTGTGATTTTTCAAAGGCCAATTCTTGGATTATTAGACGCTAGCTGTGAATGTGGTCGCTGTGAAATTTGAGATACGGGACACTACAGGTCTAGAAAGATATCATAGCTTGGCTCACATGTATTATCGTGGTGTTGCTGCAGCGATAATTGTTTATGACATCACAAATTCGGAT
TCTTTTACCCGAGCAAAGAATTGGGTGCTGGAACTTCAAAAACAAGGCAACCCAAATTTGGTTATGGCCCTTGCTGGAAACAAGGCTGATGCGGCAGCGAAGAGGAAGGTTGAAGCAGAGGAAGCTGAAACATATGCAAAGGAAAATGGTCTATTTTTCATGGAAACCTCAGCAAAGACGGCGCAAAATGTCAATGAACT
GTTCTATGAAATTGCACGGAGATTACCTAAAGCACGACCGGTGCAGCAGCCTGCAGGCATGGTTCTCACAGATAGACCTGCAGAGGGAGCTAGAACCTACTCTTGCTGCTCATAAGCAGCAAGCAGTTGTTCTATGCACATTATGTGCTACAGCTGTCATATCCGGAGGAGATGGTGGAGGGTTCAAGCTTTGTTTAGAA
CTCGATTTAAGGAATATTAGCAGTTTATGGAGAAATGTAAAAATACATATTTTGGTGTTTAAATTAAATCTTTGTACTGAGTTACGATGGTCATTGAGAGTCTTCTAATACAGCTCTTTGTGTCATTTGTGATGAGCTTATATTTAAATTGAAAGCTATGCTCTGTTTAATTACTTCGATCTCGGAGAAGAAAATCCCTT
TTGATCCTACATTGACATCTTGTGTTTGATTGTACTAGATTGTACTATGAGACGTCTAGCCTGTCATTTAAAAATATAAGTCCGTTAGTAACAAATTCTTGCTGTTAATGCCTGGTTATTGGAAATAATGTTTTTTGAAGCATGGCGAATAAATTGGCTAATAGTGTAGTTGTTCTGAACAGGTTCTGTTTTCTCCATTG
GTAGAATTGCATATTTGTAATCCTTGGACTTGGAATTGATATGATCACTCATAGTACATTACATTTCTTCATTTCTTTTTCAGAGGAGTCTTATGAATTCAGGTATAGATCTCTACTATTAAATGTTAAAATGTTATGATGCTTGTATCTCTTTCAGGA

>TRINITY_DN109691_c1_g1_i6 from sample A3 / Example unspliced isoform:

ATTTTTGTGTTTTTGTTGGGTTCAGTGCGTGGAAACTAAGGCAAGCCCAAACAGTCCAGGTGATGTAGATTCCACAAGTGTGGTGGGTGCAAGTGAAGGCTAATGAGAAGGTACAACCGTGGCCTCTTCTTTATTGGACAAGCAAGGAAGAAAGAGCTTGCTTACAGCCAGTTTTACGGGAATGGTGTTTCTTGGTGATA
TGGGTTCTGGAAAATCGAGCCTCATTTTGTGATTTTTCAAAGGCCAATTCTTGGATTATTAGgttatattcttgatttcgttactcttgtgttgttttcttttacaagctttgtttgatttttggttcaattgcttgatttaagtgtcctacatgtttttaaaagttgacaatcggagcaacattcttctcacagACGCT
AGCTGTGAATGTGGTCGCTGTGAAATTTGAGATACGGGACACTACAGGTCTAGAAAGATATCATAGCTTGGCTCACATGTATTATCGTGGTGTTGCTGCAGCGATAATTGTTTATGACATCACAAATTC