sp|Q8GXZ3|PBL8_ARATH ----> Probable serine/threonine-protein kinase PBL8

Intron 1 GTAACCATGT ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75	1	2	4
A2	60	3	3	6
A3	33.33	5	4	1
A4	38.46	4	4	1
A5	55.56	2	3	2
A6	27.27	8	2	4
A7	48.15	7	8	5
A8	52.63	9	12	8
A9	25	9	1	5
A10	71.43	2	0	10

>TRINITY_DN36013_c0_g1_i2 from sample A9 / Example spliced isoform

CCAACGTGCTGGTTTTCACATTGTTTGAACTGGAGATCATCACCAAAAGTTTCCGTTCTGATTATATTTTGGGGGAAGGAGGCTTTGGAACTGTATATAAGGGGTATATAGATGAGAATGTCAGAGCAGGCCTCAAACCTCTGCCTGTTGCAGTGAAGGTTCTCAACAAAAATGGTTTTCAAGGCCACAGGGAATGGCTG


ACGGAAGTTATATTTTTGGGGCAGCTAAGTCATCCCCATTTGGTCAAACTGATAGGGTACTGCTGCGAGGATGATCATAGATTGCTGGTTTACGAGTTTATGTTTCGTGGCAGTCTGGAGAATCATCTATTTCGGAAGGCATCCGTGACTTTGCCTTGGTCTACAAGGATGATGATTGCACTTGGGGCTGCCAAAGGGCT
TGCTTTTCTTCATGGAGCAGAAAAACCAGTTATCTATCGAGATTTCAAAACATCCAACATTTTATTGGATTCAGACTATGCAGCTAAGCTATCTGATTTTGGACTTGCAAAAGATGGTCCTCAGGGAGATGAAACTCATGTTTCGACTCGGGTGATGGGTACATATGGTTATGCAGCTCCTGAATATGTTATGACTGGGC
ACTTGACAGCTAGAAGTGATGTCTACAGCTTTGGTGTTGTGCTCCTTGAGCTGCTTACTGGAAGGCGGTCTGTGGATAAGACTAGACCAAACAGAGAACATAACCTGGTAGACTGGGCCCGTCCACATCTAAATGATAAGCGGAGGTTGCTGCAGATTATTGATCCTAGGCTGGAAGGACAGTATTCCATGAAAGGTGCC
CAGAAAGCTTGTAGCTTGGCCTATCACTGCCTTAACCAAAATCCCAAGGCAAGGCCTCTAATGAGTGATGTTGTTGAGACATTGGAACCTCTACAAAATAGGAAGGATATGGTGAATGCTTCCTTAGTGTATACTTTAAATAATGGGATAGCAGCATATAAGATGCAGAATCGTTTGAAAGAAAATGGTAGTAACATCAA
TGGTTGTCATGTTCATCACAATAATTCTCCTGCTCCTAGAGATGGACCTGCTGTCTGCAGGGTTAGGTAATAATGATTCAAGTCTCTACAAGTAATCTTCAAGGCATCCATCAACAGAAGTGGAAGTTTCTTGGACTTGAAACCCGTTATTCTTGTCTTGAAAGAGTGATTCAAACCTGTACAATCTCCGTGAGTTGCTG
TAATAAAATAATTATTTATATCTAATAGTCCCATGTGATCTTGACAAAATGCTAGTAGTTTGTAATGGCTTTGAAAGATGTACACAGCCTCATTCTTTTTCGTTGTTTGGATTGTGCGACTTTTTGTACCATCCAAGGTCACCTTGTGCTTATTACTGTGATCCAGGCCCAGTTCATATTCTTTTCCATTTGACGTTATT
CTCAAATCCTTATCTTATGCTTTTTCAATTTC

>TRINITY_DN36013_c0_g1_i4 from sample A9 / Example unspliced isoform:

CCAACGTGCTGGTTTTCACATTGTTTGAACTGGAGATCATCACCAAAAGTTTCCGTTCTGATTATATTTTGGGGGAAGGAGGCTTTGGAACTGTATATAAGGGGTATATAGATGAGAATGTCAGAGCAGGCCTCAAACCTCTGCCTGTTGCAGTGAAGGTTCTCAACAAAAATGGTTTTCAAGGCCACAGGGAATGGCTG


gtaaccatgtttttttttcttatgtattatgtgggtttttgatatgttttagtgatcatcagtttcagagcaattcaatccaatgggttcaccatagtaccaagcttatacctagacaaagatcccgacctgggttgtaatcatgattaataaatttttcgttttccccttttgtaggtttgtgggtattttgctgttca
aggctgatttctacgatttattttgcagACGGAAGTTATATTTTTGGGGCAGCTAAGTCATCCCCATTTGGTCAAACTGATAGGGTACTGCTGCGAGGATGATCATAGATTGCTGGTTTACGAGTTTATGTTTCGTGGCAGTCTGGAGAATCATCTATTTCGGAAGGCATCCGTGACTTTGCCTTGGTCTACAAGGATGA
TGATTGCACTTGGGGCTGCCAAAGGGCTTGCTTTTCTTCATGGAGCAGAAAAACCAGTTATCTATCGAGATTTCAAAACATCCAACATTTTATTGGATTCAGACTATGCAGCTAAGCTATCTGATTTTGGACTTGCAAAAGATGGTCCTCAGGGAGATGAAACTCATGTTTCGACTCGGGTGATGGGTACATATGGTTAT
GCAGCTCCTGAATATGTTATGACTGGGCACTTGACAGCTAGAAGTGATGTCTACAGCTTTGGTGTTGTGCTCCTTGAGCTGCTTACTGGAAGGCGGTCTGTGGATAAGACTAGACCAAACAGAGAACATAACCTGGTAGACTGGGCCCGTCCACATCTAAATGATAAGCGGAGGTTGCTGCAGATTATTGATCCTAGGCT
GGAAGGACAGTATTCCATGAAAGGTGCCCAGAAAGCTTGTAGCTTGGCCTATCACTGCCTTAACCAAAATCCCAAGGCAAGGCCTCTAATGAGTGATGTTGTTGAGACATTGGAACCTCTACAAAATAGGAAGGATATGGTGAATGCTTCCTTAGTGTATACTTTAAATAATGGGATAGCAGCATATAAGATGCAGAATC
GTTTGAAAGAAAATGGTAGTAACATCAATGGTTGTCATGTTCATCACAATAATTCTCCTGCTCCTAGAGATGGACCTGCTGTCTGCAGGGTTAGGTAATAATGATTCAAGTCTCTACAAGTAATCTTCAAGGCATCCATCAACAGAAGTGGAAGTTTCTTGGACTTGAAACCCGTTATTCTTGTCTTGAAAGAGTGATTC
AAACCTGTACAATCTCCGTGAGTTGCTGTAATAAAATAATTATTTATATCTAATAGTCCCATGTGATCTTGACAAAATGCTAGTAGTTTGTAATGGCTTTGAAAGATGTACACAGCCTCATTCTTTTTCGTTGTTTGGATTGTGCGACTTTTTGTACCATCCAAGGTCACCTTGTGCTTATTACTGTGATCCAGGCCCAG
TTCATATTCTTTTCCATTTGACGTTATTCTCAAATCCTTATCTTATGCTTTTTCAATTTC

Intron 2 GTTGTAATCA ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	0	4
A2	100	0	2	6
A3	33.33	1	0	1
A4	100	0	4	1
A5	100	0	0	2
A6	50	3	2	4
A7	60	3	4	5
A8	65.22	4	7	8
A9	44.83	8	8	5
A10	90.48	1	9	10

>TRINITY_DN39353_c0_g1_i1 from sample A8 / Example spliced isoform

GTTGGATATACATTGCCACTGCGGCATGCTCCATTGTGGAATATTGACATATTGTATTATGTTAAAACTCCTCAACTCTGTAACTACAAATGTATTCATTTCTGAGAGTGTTGATCTACTGTTATTTATCAGGGCCATGTTTTGTTTTTTAGGCACAGTCCAGACACAGCCGGTACCATTGGCACATAAACAAGAACAGC
AACGCAAGAAAGAGCAGCAAGACCCATCTCCTGCAATAAGCAAAAAACCCACCCCCGTTAAGAGACTATCCTTGTCTTCATCAGATCTGAGCAATCCATCAACTCCCAGGGCTGTAGAAGACCTGAGACAGGACTCTGGATCCAACGTGCTGGTTTTCACATTGTTTGAACTGGAGATCATCACCAAAAGTTTCCGTTCC
GATTATATTTTGGGGGAAGGAGGCTTTGGAACTGTATATAAGGGGTATATAGATGAGAATGTCAGAGCAGGCCTCAAACCTCTGCCTGTTGCAGTGAAGGTTCTCAACAAAAATGGTTTTCAAGGCCACAGGGAATGGCTGGTAACCATGTTTTTTTTCTTATGTATTATGTGGGTTTTTGATATGTTTTAGTGATCATC
AGTTTCAGAGCAATTCAATCCAATGGGTTCACCATAGTACCAAGCTTATACCTAGACAAAGATCCCGACCTGGACGGAAGTTATATTTTTGGGGCAGCTAAGTCATCCCCATTTGGTCAAACTGATAGGGTACTGCTGCGAGGATGATCATAGATTGCTGGTTTACGAGTTTATGTTTCGTGGCAGTCTGGAGAATCATC
TATTTCGGAGTATGTATTTGCCGCCTTACTTACTGGCTGTGTATGTTTAATCAGAACTAACAGTTCAATTATATATAGATATATTTTGTCTTTCTAAATTTATTGAATTCCTGAAATTTGGAACAAGACCTATGTAAATTGTTTCGGCCTCGAACTCCTTGACGCATCATTCTATAGATCGTTTCGACTAATCTAGCTGT
CTTGGTCTGAACCGGGAGATGTAGTATTGTATAGTATGCATTATGGATTTCAATTGGTCATTTAAGCATGCATAATGGTTGAC

>TRINITY_DN39353_c0_g1_i2 from sample A8 / Example unspliced isoform:

GTTGGATATACATTGCCACTGCGGCATGCTCCATTGTGGAATATTGACATATTGTATTATGTTAAAACTCCTCAACTCTGTAACTACAAATGTATTCATTTCTGAGAGTGTTGATCTACTGTTATTTATCAGGGCCATGTTTTGTTTTTTAGGCACAGTCCAGACACAGCCGGTACCATTGGCACATAAACAAGAACAGC
AACGCAAGAAAGAGCAGCAAGACCCATCTCCTGCAATAAGCAAAAAACCCACCCCCGTTAAGAGACTATCCTTGTCTTCATCAGATCTGAGCAATCCATCAACTCCCAGGGCTGTAGAAGACCTGAGACAGGACTCTGGATCCAACGTGCTGGTTTTCACATTGTTTGAACTGGAGATCATCACCAAAAGTTTCCGTTCC
GATTATATTTTGGGGGAAGGAGGCTTTGGAACTGTATATAAGGGGTATATAGATGAGAATGTCAGAGCAGGCCTCAAACCTCTGCCTGTTGCAGTGAAGGTTCTCAACAAAAATGGTTTTCAAGGCCACAGGGAATGGCTGGTAACCATGTTTTTTTTCTTATGTATTATGTGGGTTTTTGATATGTTTTAGTGATCATC
AGTTTCAGAGCAATTCAATCCAATGGGTTCACCATAGTACCAAGCTTATACCTAGACAAAGATCCCGACCTGGgttgtaatcatgattaataaatttttcgttttccccttttgtaggtttgtgggtattttgctgttcaaggctgatttctacgatttattttgcagACGGAAGTTATATTTTTGGGGCAGCTAAGTCA
TCCCCATTTGGTCAAACTGATAGGGTACTGCTGCGAGGATGATCATAGATTGCTGGTTTACGAGTTTATGTTTCGTGGCAGTCTGGAGAATCATCTATTTCGGAAGGCATCCGTGACTTTGCCTTGGTCTACAAGGATGATGATTGCACTTGGGGCTGCCAAAGGGCTTGCTTTTCTTCATGGAGCAGAAAAACCAGTTA
TCTATCGAGATTTCAAAACATCCAACATTTTATTGGATTCAGACTATGCAGCTAAGCTATCTGATTTTGGACTTGCAAAAGATGGTCCTCAGGGAGATGAAACTCATGTTTCGACTCGGGTGATGGGTACATATGGTTATGCAGCTCCTGAATATGTTATGACTGGGCACTTGACAGCTAGAAGTGATGTCTACAGCTTT
GGTGTTGTGCTCCTTGAGCTGCTTACTGGAAGGCGGTCTGTGGATAAGACTAGACCTAACAGAGAACATAACCTGGTAGACTGGGCCCGTCCACATCTAAATGATAAGCGGAGGTTGCTGCAGATTATTGATCCTAGGCTGGAAGGACAGTATTCCATGAAAGGTGCCCAGAAAGCTTGTAGCTTGGCCTATCACTGCCT
TAACCAAAATCCCAAGGCAAGGCCTCTAATGAGTGATGTTGTTGAGACATTGGAACCTCTACAAAATAGGAAGGATATGGTGAATGCTTCCTTAGTGTATACTTTAAATAATGGGATAGCAGCATATAAGATGCAGAATCGTTTGAAAGAAAATGGTAGTAACATCAATGGTTGTCATGTTCATCACAATAATTCTCCTG
CTCCTAGAGATGGACCTGCTGTCTGCAGGGTTAGGTAATAATGATTCAAGTCTCTACAAGTAATCTTCAAGGCATCCATCAACAGAAGTGGAAGTTTCTTGGACTTGAAACCCGTTATTCTTGTCTTGAAAGAGTGATTCAAACCTGTACAATCTCCGTGAGTTGCTGTAATAAAATAATTATTTATATCTAATAGTCCC
ATGTGATCTTGACAAAATGCTAGTAGTTTGTAATGGCTTTGAAAGATGTACACAGCCTCATTCTTTTTCGTTGTTTGGATTGTGCGACTTTTTGTACCATCCAAGGTCACCTTGTGCTTATTACTGTGATCCAGGCCCAGTTCATATTCTTTTC

Intron 3 GTAACCATGT ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75	1	2	4
A2	60	3	3	6
A3	33.33	5	4	1
A4	38.46	4	4	1
A5	55.56	2	3	2
A6	27.27	8	2	4
A7	48.15	7	8	5
A8	52.63	9	12	8
A9	21.74	9	0	5
A10	71.43	2	0	10

>TRINITY_DN40047_c0_g1_i4 from sample A7 / Example spliced isoform

CCCACACTCTATGTGGCTAAGAGAATATCCGCTTTTTATCTGAGCTAACTTGCTAGCTAATAAGGTCACGGTCTTACATTCTAGGTTGGATATACATTGCCACTGCGGCATGCTCCATTGTGGAATATTGGCATATTGTATTATGTTAAAACTCCTCAACTCTGTAACTACAAATGTATTCATTTCTGAGAGTGTTGATC
TACTGTTATTTATCAGGGCCATGTTTTGTTTTTTAGGCACAGTCCAGACACAGCCGGTACCATTGGCACATAAACAAGAACAGCAACGCAAGAAAGAGCAGCAAGACCCATCTCCTGCAATAAGCAAAAAACCCACCCCCGTTAAGAGACTATCCTTGTCTTCATCAGATCTGAGCAATCCATCAACTCCCAGGGCTGTA
GAAGACCTGAGACAGGACTCTGGATCCAACGTGCTGGTTTTCACATTGTTTGAACTGGAGATCATCACCAAAAGTTTCCGTTCCGATTATATTTTGGGGGAAGGAGGCTTTGGAACTGTATATAAGGGGTATATAGATGAGAATGTCAGAGCAGGCCTCAAACCTCTGCCTGTTGCAGTGAAGGTTCTCAACAAAAATGG
TTTTCAAGGCCACAGGGAATGGCTGACGGAAGTTATATTTTTGGGGCAGCTAAGTCATCCCCATTTGGTCAAACTGATAGGGTACTGCTGCGAGGATGATCATAGATTGCTGGTTTACGAGTTTATGTTTCGTGGCAGTCTGGAGAATCATCTATTTCGGAAGGCATCCGTGACTTTGCCTTGGTCTACAAGGATGATGA
TTGCACTTGGGGCTGCCAAAGGGCTTGCTTTTCTTCATGGAGCAGAAAAACCAGTTATCTATCGAGATTTCAAAACATCCAACATTTTATTGGATTCAGACTATGCAGCTAAGCTATCTGATTTTGGACTTGCAAAAGATGGTCCTCAGGGAGATGAAACTCATGTTTCGACTCGGGTGATGGGTACATATGGTTATGCA
GCTCCTGAATATGTTATGACTGGGCACTTGACAGCTAGAAGTGATGTCTACAGCTTTGGTGTTGTGCTCCTTGAGCTGCTTACTGGAAGGCGGTCTGTGGATAAGACTA

>TRINITY_DN40047_c0_g1_i2 from sample A7 / Example unspliced isoform:

AGATGGCGGAAAACCCACTCCTGCAAAGTCACGGGATTCATGTATCAGACGACAGCCCCAATAAATTGAGTTGATTTCTATTTCAATTATTAATTTCTGCGTAATAATTACGTTTACTTCATAAATCGACGGTAGATTGTACTCAAATTGCCTTCTGTTCCACCCAATTCCAGCGTTTTCTGTTCTGTCCAATGACGGAG
ATCCAATGGCAGAGCTGCAGATTTTCACTGGGGGCACTGAATTCCTAAAAAAACTGCAAAGGATCTGCATGATGTGTGTACTTCCCGTATCAGAAAACACGGCTCAAAGACAAGGTCACATGGAGTTGGGCGCTTAGCGAAGGAGCTTATTGGCCCATGTTGTAGGCTTTTTCAGGAGAAGAAGCCACAGCACTCTTGTG
AGCATGTCAGCGCATTGCTGTTCATCTGCAAACCCAAACACAGATTTTGACAGCCCATTTGCAGTGAGTATGTGACCACTTTGCCATGGATCTTGAAGCCAAAACCCAGATTTTAAGGGCCCATTTGCTGTCGGCATGTGACCACATTGGCCTTTGTCTACAACCCCAATCTCAGTGGTTGCAATAGTGGAGCTAGGAGG
GAGGATGCATTTGGATTGAGTTTGATCATGGGAAACTGTGGAACAAGAGAGGAGTCTACTGTCACCAGTGTTGTCCCCATAGCTAAGCCAGGCACAGTCCAGACACAGCCGGTACCATTGGCACATAAACAAGAACAGCAACGCAAGAAAGAGCAGCAAGACCCATCTCCTGCAATAAGCAAAAAACCCACCCCCGTTAA
GAGACTATCCTTGTCTTCATCAGATCTGAGCAATCCATCAACTCCCAGGGCTGTAGAAGACCTGAGACAGGACTCTGGATCCAACGTGCTGGTTTTCACATTGTTTGAACTGGAGATCATCACCAAAAGTTTCCGTTCCGATTATATTTTGGGGGAAGGAGGCTTTGGAACTGTATATAAGGGGTATATAGATGAGAATG
TCAGAGCAGGCCTCAAACCTCTGCCTGTTGCAGTGAAGGTTCTCAACAAAAATGGTTTTCAAGGCCACAGGGAATGGCTGgtaaccatgttttttttcttatgtattatgtgggtttttgatatgttttagtgatcatcagtttcagagcaattcaatccaatgggttcaccatagtaccaagcttatacctagacaaag
atcccgacctgggttgtaatcatgattaataaatttttcgttttccccttttgtaggtttgtgggtattttgctgttcaaggctgatttctacgatttattttgcagACGGAAGTTATATTTTTGGGGCAGCTAAGTCATCCCCATTTGGTCAAACTGATAGGGTACTGCTGCGAGGATGATCATAGATTGCTGGTTTAC
GAGTTTATGTTTCGTGGCAGTCTGGAGAATCATCTATTTCGGAAGGCATCCGTGACTTTGCCTTGGTCTACAAGGATGATGATTGCACTTGGGGCTGCCAAAGGGCTTGCTTTTCTTCATGGAGCAGAAAAACCAGTTATCTATCGAGATTTCAAAACATCCAACATTTTATTGGATTCAGACTATGCAGCTAAGCTATC
TGATTTTGGACTTGCAAAAGATGGTCCTCAGGGAGATGAAACTCATGTTTCGACTCGGGTGATGGGTACATATGGTTATGCAGCTCCTGAATATGTTATGACTGGGCACTTGACAGCTAGAAGTGATGTCTACAGCTTTGGTGTTGTGCTCCTTGAGCTGCTTACTGGAAGGCGGTCTGTGGATAAGACTA