NA ----> NA

Intron 1 GTAATTTCTA ... TCGCAGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.72	24	31.3	34.9
A2	49.64	25	32.2	17.5
A3	52.36	20.5	22.4	22.7
A4	66.41	9	18.6	17
A5	52.64	9	10.5	9.6
A6	57.12	81	98	118.3
A7	56.88	83.5	102.5	118.4
A8	55.76	77.5	86.8	108.9
A9	60.26	59	83.3	96
A10	61.59	36	50.9	64.8

>TRINITY_DN110851_c0_g1_i3 from sample A3 / Example spliced isoform

AACTAAAAATTCCACACTATCTGCTTCTTCGTTGATTTGGTTTGTTTAATTATCATTTATCATCTATTAATGATGAGGTTACAAATCTGCAGCTTTACACGAAAGAACAGAAAAATGTTAGTTTTGTAAAAGAGGTTCTAGAGACTATGGAAAAGAATTTCATAAAACCAGAGCCAACCACTGCTGATATCATTGTTAGC
ATATGCAATGACACTGAACACTGGTCGTTGATGTCTAATATTGCTAAAAAGTTCATCAAGAATGGGGTAAAGCTTCATCCACGTGCTTATGACATATGGATTTCTTCTGAAGCCAATTTAGGGAATACTCTAAAGGTTTGGAAAATACAGGAATTGAGGTTGCTGTTTGGATTACCACATACAATTCCAAGTGCATTCTC
TTGTGCCAAGGCCTATCTATTGAAGTGTCAACCTCAGGAAGCAGCTGAACTCATTCTTGAGCTCAATCAGGAGGATCTCTCTGATTCCAAGAAGAAGTGGGTTTATGAAGAACTACAATATCTAGTGGACGAGTGGCCTGCAGAGGCCCTTGCTACTGAGTTGAAAGATTGCATCAGTATGATGTTCAACTATCTTCTTA
CCAAGGGCATAAATCTTTCATTGGATGTCGAAGAGAGGTATTGCTCAAAAAGCACTAGGAGCATGGATGAAATTATACTAGGCTGAATAATGAGTCTTTGTGGGCCATTGGTATCCAACTCTGTCAATATAGGTACAAACTAAGTGGAGGTCAGTATTTAAGCTGACGTTTTCAATGAACCAGTATAACTTCTCTTGATC
ATATCTCAAAGGGTTTGACAAATATTTCAGGGCATATCAATCTAATCATCAGTTATTTACTTTTTGTGACATTCCACTGTACAATGAACATCCCTATGAGAAAGTCAACCGCACATTTCCAAAAGACTCTGTTGATGTGCTTCTCTTTGGGTTCATCTTAAAATTGAATGCCATGTCATTGAAATCTATTAGTGCAAGAC
AAAAACCACAAATGGTGCTTTCCCTACTTTGATGTTTAGGTTTCTATATGGTAACAATAATTGATAACCTTAACATGGTATTTTTAAA

>TRINITY_DN110851_c0_g1_i8 from sample A3 / Example unspliced isoform:

GTCGTTTGTGATCGGGAGGTTTAGGAAGGGTTTATGAAATTCTTAATGTGCCTTTGTTACTGTGCATAGCACTGAGAACTGAGGGGATTTCGCTGTTTTGGATTTCTGGGCCGAGAGAAAGAGAAGGCCGAAGTAAGCAAGGGTCGTAAAATGCAGGTCGCAGTGAGCAGAGCCGTCTACAAGGCTCTGGGAAGGCCATT
TATTTATTATTCAATACAAACGATTCCATCATCATCTCCATCCCCGAAATGTCCAGACGACGAGCGATCGAATCTCTGCAGGAGCTTCTGCACGTTTGTCTCAAGGAATAATACACAGGATATTAATTGCCGGTATTTGGCGGGCAGGAGAATGCAGCGGTTGTATTTGTCCACAGAGGCGGAGATTCTAAAAGAAGGCC
CTATAAAAGAAGAGCCTACAGAAGCTGTACAGGAGTTGTACCTTAAAATTATTGACTCAGTGAAGGCTGGAGCTATGCCTCCAAAAACTTGGCGGGATTCATTACTGAAAAACTGTAAGAACAAAGAAGATTTGGAGTTGGCTTTTGAAGTATTGGAGAAGCTTCGGAAATTTAAAACGTCAAAGTTATGGCAGTCTTCC
AACTGGAGTCAACAGATTACTTTAGCAGTTGTTTCTGCATGCATTCGTGCAAATTCACCACACCTTGGATTAAGAACCCTCCAGAGACACAATGTTTATGGTCTCACGCCATGTGTTGGTGCTGCTCATAGTTTACTGCTTTACACGAAAGAACAGAAAAATGTTAGTTTTGTAAAAGAGGTTCTAGAGACTATGGAAAA
GAATTTCATAAAACCAGAGCCAACCACTGCTGATATCATTGTTAGCATATGCAATGACACTGAACACTGGTCGTTGATGTCTAATATTGCTAAAAAGTTCATCAAGAATGGGGTAAAGCTTCATCCACGTGCTTATGACATATGGATTTCTTCTGAAGCCAATTTAGGGAATACTCTAAAGGTTTGGAAAATACAGGAAT
TGAGGTTGCTGTTTGGATTACCACATACAATTCCAAGTGCATTCTCTTGTGCCAAGGCCTATCTATTGAAGTGTCAACCTCAGGAAGCAGCTGAACTCATTCTTGAGCTCAATCAGGAGGATCTCTCTGATTCCAAGAAGAAGTGGGTTTATGAAGAACTACAATATCTAGTGGACGAGTGGCCTGCAGAGgtgatttct
aggcgagataaaagtcgcaggaagGCCCTTGCTACTGAGTTGAAAGATTGCATCAGTATGATGTTCAACTATCTTCTTACCAAGGGCATAAATCTTTCATTGGATGTCGAAGAGAGGATTAAGAACCCTCCAGAGACACAATGTTTATGGTCTCACGCCATGTGTTGGTGCTGCTCATAGTTTACTGCTTTACACGAAAG
AACAGAAAA

Intron 2 GTAATTATTA ... CTTGTCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.32	33.8	10	3
A2	12.11	40	4	7
A3	27.36	21.3	7	9
A4	13.8	18.8	4	2
A5	21.94	12.5	5	2
A6	17	88.8	17	19
A7	17.97	92	17	23
A8	22.74	80.5	21	26
A9	20.72	90.8	25	22
A10	25.86	55.8	16	22

>TRINITY_DN110851_c0_g1_i3 from sample A3 / Example spliced isoform

AACTAAAAATTCCACACTATCTGCTTCTTCGTTGATTTGGTTTGTTTAATTATCATTTATCATCTATTAATGATGAGGTTACAAATCTGCAGCTTTACACGAAAGAACAGAAAAATGTTAGTTTTGTAAAAGAGGTTCTAGAGACTATGGAAAAGAATTTCATAAAACCAGAGCCAACCACTGCTGATATCATTGTTAGC
ATATGCAATGACACTGAACACTGGTCGTTGATGTCTAATATTGCTAAAAAGTTCATCAAGAATGGGGTAAAGCTTCATCCACGTGCTTATGACATATGGATTTCTTCTGAAGCCAATTTAGGGAATACTCTAAAGGTTTGGAAAATACAGGAATTGAGGTTGCTGTTTGGATTACCACATACAATTCCAAGTGCATTCTC
TTGTGCCAAGGCCTATCTATTGAAGTGTCAACCTCAGGAAGCAGCTGAACTCATTCTTGAGCTCAATCAGGAGGATCTCTCTGATTCCAAGAAGAAGTGGGTTTATGAAGAACTACAATATCTAGTGGACGAGTGGCCTGCAGAGGCCCTTGCTACTGAGTTGAAAGATTGCATCAGTATGATGTTCAACTATCTTCTTA
CCAAGGGCATAAATCTTTCATTGGATGTCGAAGAGAGGTATTGCTCAAAAAGCACTAGGAGCATGGATGAAATTATACTAGGCTGAATAATGAGTCTTTGTGGGCCATTGGTATCCAACTCTGTCAATATAGGTACAAACTAAGTGGAGGTCAGTATTTAAGCTGACGTTTTCAATGAACCAGTATAACTTCTCTTGATC
ATATCTCAAAGGGTTTGACAAATATTTCAGGGCATATCAATCTAATCATCAGTTATTTACTTTTTGTGACATTCCACTGTACAATGAACATCCCTATGAGAAAGTCAACCGCACATTTCCAAAAGACTCTGTTGATGTGCTTCTCTTTGGGTTCATCTTAAAATTGAATGCCATGTCATTGAAATCTATTAGTGCAAGAC
AAAAACCACAAATGGTGCTTTCCCTACTTTGATGTTTAGGTTTCTATATGGTAACAATAATTGATAACCTTAACATGGTATTTTTAAA

>TRINITY_DN110851_c0_g1_i7 from sample A3 / Example unspliced isoform:

GTCGTTTGTGATCGGGAGGTTTAGGAAGGGTTTATGAAATTCTTAATGTGCCTTTGTTACTGTGCATAGCACTGAGAACTGAGGGGATTTCGCTGTTTTGGATTTCTGGGCCGAGAGAAAGAGAAGGCCGAAGTAAGCAAGGGTCGTAAAATGCAGGTCGCAGTGAGCAGAGCCGTCTACAAGGCTCTGGGAAGGCCATT
TATTTATTATTCAATACAAACGATTCCATCATCATCTCCATCCCCGAAATGTCCAGACGACGAGCGATCGAATCTCTGCAGGAGCTTCTGCACGTTTGTCTCAAGGAATAATACACAGGATATTAATTGCCGGTATTTGGCGGGCAGGAGAATGCAGCGGTTGTATTTGTCCACAGAGGCGGAGATTCTAAAAGAAGGCC
CTATAAAAGAAGAGCCTACAGAAGCTGTACAGGAGTTGTACCTTAAAATTATTGACTCAGTGAAGGCTGGAGCTATGCCTCCAAAAACTTGGCGGGATTCATTACTGAAAAACTGTAAGAACAAAGAAGATTTGGAGTTGGCTTTTGAAGTATTGGAGAAGCTTCGGAAATTTAAAACGTCAAAGTTATGGCAGTCTTCC
AACTGGAGTCAACAGATTACTTTAGCAGTTGTTTCTGCATGCATTCGTGCAAATTCACCACACCTTGGATTAAGAACCCTCCAGAGACACAATGTTTATGGTCTCACGCCATGTGTTGGTGCTGCTCATAGTTTACTGCTTTACACGAAAGAACAGAAAAATGTTAGTTTTGTAAAAGAGGTTCTAGAGACTATGGAAAA
GAATTTCATAAAACCAGAGCCAACCACTGCTGATATCATTGTTAGCATATGCAATGACACTGAACACTGGTCGTTGATGTCTAATATTGCTAAAAAGTTCATCAAGAATGGGGTAAAGCTTCATCCACGTGCTTATGACATATGGATTTCTTCTGAAGCCAATTTAGGGAATACTCTAAAGGTTTGGAAAATACAGGAAT
TGAGGTTGCTGTTTGGATTACCACATACAATTCCAAGTGCATTCTCTTGTGCCAAGGCCTATCTATTGAAGTGTCAACCTCAGGAAGCAGCTGAACTCATTCTTGAGCTCAATCAGgtaattattatattatcaacaagagttgtgttggatttagacacacaggtgagcaaatgtgtaaatgctgctatctctgacttg
tcatagGAGGATCTCTCTGATTCCAAGAAGAAGTGGGTTTATGAAGAACTACAATATCTAGTGGACGAGTGGCCTGCAGAGGTGATTTCTAGGCGAGATAAAAGTCGCAGGAAGGCCCTTGCTACTGAGTTGAAAGATTGCATCAGTATGATGTTCAACTATCTTCTTACCAAGGGCATATATCTTTCATTGGATGTCGA
AGAAAGGTATTGCTCAAAAAGCACTAGGAGCATGGATGAAATTATACTAGGCTGAATAATGAGTCTTTGTGGGCCATTGGTATCCAACTCTGTCAATATAGGTACAAACTAAGTGGAGGTCAGTATTTAAGCTGACGTTTTCAATGAACCAGTATAACTTCTCTTGATCATATCTCAAAGGGTTTGACAAATATTTCAGG
GCATATCAATCTGACCATCAGTTATTTACTTTTTGTGACATTCCACTGTACAATGAACATCCCTATGAGAAAGTCAACCGCACATTTCCAAAAGACTCTGTTGATGTGCTTTTCTTTGGGTTCGTCTTAAAATTGAATGCCATGTCATTGAAATCTATTAGTGCAAGACAAAAACCACAAATGGTGCTTTCCCTACTTTG
ATGTTTAGGTTTCTATATGGTAACAATAATTGATAACCTTAACATGGTATTTTTAAA

Intron 3 GTAAACTCAA ... TTGTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.92	37	11	0
A2	21.74	18	5	0
A3	14.81	23	4	0
A4	10.53	17	2	0
A5	16.67	10	2	0
A6	14.69	122	21	0
A7	19.21	122	29	0
A8	18.84	112	26	0
A9	20.16	99	25	0
A10	25.56	67	23	1

>TRINITY_DN43692_c0_g1_i1 from sample A10 / Example spliced isoform

GTGATCGGGAGGTTTAGGAAGGGTTTATGAAATTCTTAATGTGCCTTTGTTACTGTGCATAGCACTGAGAACTGAGGGGATTTCGCTGTTTTGGATTTCTGGGCCGAGAGAAAGAGAAGGCCGAAGTAAGCAAGGGTCGTAAAATGCAGGTCGCAGTGAGCAGAGCCGTCTACAAGGCTCTGGGAAGGCCATTTATTTAT
TATTCAATACAAACGATTCCATCATCATCTCCATCCCCGAAATGTCCAGACGACGAGCGATCGAATCTCTGCAGGAGCTTCTGCACGTTTGTCTCAAGGAATAATACACAGGGATTATTTTTGGCAGATATTAATTGCCGGTATTTGGCGGGCAGGAGAATGCAGCGGTTGTATTTGTCCACAGAGGCGGAGATTCTAAA
AGAAGGCCCTATAAAAGAAGAGCCTACAGAAGCTGTACAGGAGTTGTACCTTAAAATTATTGACTCAGTGAAGGCTGGAGCTATGCCTCCAAAAACTTGGCGGGATTCATTACTGAAAAACTGTAAGAACAAAGAAGATTTGGAGTTGGCTTTTGAAGTATTGGAGAAGCTTCGGAAATTTAAAACGTCAAAGTTATGGC
AGTCTTCCAACTGGAGTCAACAGATTACTTTAGCAGTTGTTTCTGCATGCATTCGTGCAAATTCACCACACCTTGGATTAAGAACCCTCCAGAGACACAATGTTTATGGTCTCACGCCATGTGTTGGTGCTGCTCATAGTTTACTGCTTTACACGAAAGAACAGAAAAATGTTAGTTTTGTAAAAGAGGTTCTAGAGACT
ATGGAAAAGAATTTCATAAAACCAGAGCCAACCACTGCTGATATCATTGTTAGCATATGCAATGACACTGAACACTGGTCGTTGATGTCTAATATTGCTAAAAAGTTCATCAAGAATGGGGTAAAGCTTCATCCACGTGCTTATGACATATGGATTTCTTCTGAAGCCAATTTAGGGAATACTCTAAAGGTTTGGAAAAT
ACAGGAATTGAGGTTGCTGTTTGGATTACCACATACAATTCCAAGTGCATTCTCTTGTGCCAAGGCCTATCTATTGAAGTGTCAACCTCAGGAAGCAGCTGAACTCATTCTTGAGCTCAATCAGGAGGATCTCTCTGATTCCAAGAAGAAGTGGGTTTATGAAGAACTACAATATCTAGTGGACGAGTGGCCTGCAGAGG
TGATTTCTAGGCGAGATAAAAGTCGCAGGAAGGCCCTTGCTACTGAGTTGAAAGATTGCATCAGTATGACGTTCAACTATCTTCTTACCAAGGGCATATATCTTTCATTGGATGTCGAAGAAAGGTATTGCTCAAAAAGCACTAGGAGCATGGATGAAATTATACTAGGCTGAATAATGAGTCTTTGTGGGCCATTGGTA
TCCAACTCTGTCAATATAGGTACAAACTAAGTGGAGGTCAGTATTTAAGCTGACGTTTTCAATGAACCAGTATAACTTCTCTTGATCATATCTCAAAGGGTTTGACAAATATTTCAGGGCATATCAATCTGACCATCAGTTATTTACTTTTTGTGACATTCCACTATACAATGAACATCCCTATGAGAAAGTCAACCGCA
CATTTCCAAAAGACTCTGTTGATGTGCTTTTCTTTGGGTTCGTCTTAAAATTGAATGCCATGTCATTGAAATCTATTAGTGCAAGACAAAAACCACAAATGGTGCTTTCCCTACTTTGATGTTTAGGTTTCTATATGGTAACAATAATTGATAACCTTAACATGGTATTTTTAAAGAGATCTGTATTTAATAAAAAATAT
AATTTTGTAG

>TRINITY_DN43692_c0_g1_i3 from sample A10 / Example unspliced isoform:

GTGATCGGGAGGTTTAGGAAGGGTTTATGAAATTCTTAATGTGCCTTTGTTACTGTGCATAGCACTGAGAACTGAGGGGATTTCGCTGTTTTGGATTTCTGGGCCGAGAGAAAGAGAAGGCCGAAGTAAGCAAGGGTCGTAAAATGCAGGTCGCAGTGAGCAGAGCCGTCTACAAGGCTCTGGGAAGGCCATTTATTTAT
TATTCAATACAAACGATTCCATCATCATCTCCATCCCCGAAATGTCCAGACGACGAGCGATCGAATCTCTGCAGGAGCTTCTGCACGTTTGTCTCAAGGAATAATACACAGGATATTAATTGCCGGTATTTGGCGGGCAGGAGAATGCAGCGGTTGTATTTGTCCACAGAGGCGGAGATTCTAAAAGAAGGCCCTATAAA
AGAAGAGCCTACAGAAGCTGTACAGGAGTTGTACCTTAAAATTATTGACTCAGTGAAGGCTGGAGCTATGCCTCCAAAAACTTGGCGGGATTCATTACTGAAAAACTGTAAGAACAAAGAAGATTTGGAGTTGGCTTTTGAAGTATTGGAGAAGCTTCGGAAATTTAAAACGTCAAAGTTATGGCAGTCTTCCAACTGGA
GTCAACAGATTACTTTAGCAGTTGTTTCTGCATGCATTCGTGCAAATTCACCACACCTTGGATTAAGAACCCTCCAGAGACACAATGTTTATGGTCTCACGCCATGTGTTGGTGCTGCTCATAGTTTACTGCTTTACACGAAAGAACAGAAAAATGTTAGTTTTGTAAAAGAGGTTCTAGAGACTATGGAAAAGAATTTC
ATAAAACCAGAGCCAACCACTGCTGATATCATTGTTAGCATATGCAATGACACTGAACACTGGTCGTTGATGTCTAATATTGCTAAAAAGTTCATCAAGAATGGGGTAAAGCTTCATCCACGTGCTTATGACATATGGATTTCTTCTGAAGCCAATTTAGGGAATACTCTAAAGGTTTGGAAAATACAGGAATTGAGGTT
GCTGTTTGGATTACCACATACAATTCCAAGTGCATTCTCTTGTGCCAAGGCCTATCTATTGAAGTGTCAACCTCAGGAAGCAGCTGAACTCATTCTTGAGCTCAATCAGGAGGATCTCTCTGATTCCAAGAAGAAGTGGGTTTATGAAGAACTACAATATCTAGTGGACGAGTGGCCTGCAGAGGTGATTTCTAGGCGAG
ATAAAAGTCGCAGGAAGgtaaactcaaatgcaccataatttgcaaaatgaatcctctcttttactctatatctttatattcatcattcatcatgaagtctcttcttgtttacagGCCCTTGCTACTGAGTTGAAA