sp|Q9SJQ9|ALFC6_ARATH ----> Fructose-bisphosphate aldolase 6, cytosolic

Intron 1 GTAAGTCATT ... TATTTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.26	40	308	240
A2	85.52	53	345	281
A3	86.87	44	331	251
A4	89.01	30	261	225
A5	88.8	21	188	145
A6	97.59	119	5014	4620
A7	94.37	155	2756	2440
A8	97.24	102	3461	3734
A9	96	125	2932	3069
A10	94.96	91	1837	1595

>TRINITY_DN98876_c0_g1_i10 from sample A4 / Example spliced isoform

TACGGCCTCACAAATTTCGTCTGTATGGCCGTTGAAGGCCAGCTTTGGTTGTCATGCTCTGCTTTGGATGAAATCCAATTTGAAGCTTTTGACAGGTTTTAGGCTCGTATGTTTGAGCAATAAGCAGCGTCACTAAAAATTATTCAGATATAAAAAAAATGTACTTCGAGTTGGATAATGCCAACTCTTATAAAAATGAA
TCTGTTGAACACCAAAGATTCGTCCAGGTCCCAGGATGAACGAAGCATCGAATTAACTGATGGTCAAATCAAAGCTCAAGAAGATTTTTCAACGGCAATTTCCAGACGTTTTGAGGAGTTCCATTTTTTGAAACTCCCTGCCCAATCCCTCTCACGCGTATTCAAGTAGCCCTCGTCTTTTGTTTTCTGCGAATTTCTCG
AACCTCATCCAATAATTTGAATCCGATGCCTTGATTTGCTAGTGGATTGTGTGCTTACGATGGTTCTACTTTATGTTTTTATCATATTTTGCAGGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATT
ACATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGAGGTTGCCTAAATGTTCAGTGAAAATAAGCGGGAATATAACAAAAGAGTTCATCAAATTGCGGAGCAGAGTTGGATTGCAAAAGTCACAACCATGCCTATTCATAGTGTAT
ACAAGGATGATAACGACGAACTTGACAGTGAAGGGCTCTTCAGGCTAGTAACTTGAAGACATGGGCTGGAAAAGTTGAGAATATCCCCTTGGCACATGATGCTTTCTTATCATGATGCCACTTTGGTCAAGTATGTGGGTGATTATTCCAAGGGAAATAGAGTTTTTGAGAGCTTTCATGTCAACAACTATAAATATTGA
TTGATGATCACTACTAGCCATCTGTCCATCAAGGAAAAGAGAGACCTCAAAGAGGAGATATTTCTTTGAATTCCAATTCAGTGGGTTGTCAGGAGTTGACAGAGCAGCTTAGCATATGATTGCTCAATGAACGGTTTACTCATCCTACAATGCAGGATTCACTTCATCCTACAATGCAGGATTCACTTGAGTCAATTTTC
CCAAAGGACACTCTTAAGAACTCACGATTTACCATCAACATTTTCACCTCTATTGGTCTTGGTGGTATCACTCAAAGTTTGCGTGAATGTTTGAAGAATATGCAAAGACTTATATTGCAGCAGCTGAAGGCCATTTCTGAATCAGATGCCTCAATTTCTGGCGTTGATTCCTTTGCTTCTACTAAACATAGCTAGAAAAA
CTCTATACGACCACTTCACACAAATGAAATGATTAGGTTGACCTTGTTGACCCATGCTAATTGATGCATTGATAAAAGAATTTTTGCTCTTCGCATTCATAGGATTACCATTTCATCCAACAAATATCTTATGGGAAATTTGTAATTCTGATTTCAGATATAAAACTTATTAATCTACG

>TRINITY_DN98876_c0_g1_i4 from sample A4 / Example unspliced isoform:

GTCTTGACCGTCCATACTGGTTTCTTAAACCTTTATGTGACCTATATGCCAAATCTTAGTTTAAATTGGCATATTGTGGAGCTAATCTAGAATTTGTATGCAATTCTTTGTTGCACTTTCTGTAATTATGTTAAGAATATTACATAAATAACTTAGCTAGTTTTGTTGTCCTTACTTTGTGATCCAAATCCTAACTCTCT
AGCAAATTCAGAGGTTGCCTAAATGTTCAGTGAAAATAAGCGGGAATATAACAAAAGAGTTCATCAAATTGCGGAGCAGAGTTGGATTGCAAAgtaagtcattttgttttttacttaagaaaaaggtttgtaatattgttaagaggaattgatggacctcatatatcaatgaaactagacttatgatactatgaatcaag
tgaacgattccaagctttgtcttatatgctcaaagacttggggtaagtctatttttatgtgttgccttttttatttgtcagAGTCACAACCATGCCTATTCATAGTGTATACAAGGATGATAACGACGAACTTGACAGTGAAGGGCTCTTCAGGCTAGTAACTTGAAGACATGGGCTGGAAAAGTTGAGAATATCCCCTT
GGCACATGATGCTTTCTTATCATGATGCCACTTTGGTCAAGTATGTGGGTGATTATTCCAAGGGAAATAGAGTTTTTGAGAGCTTTCATGTCAACAACTATAAATATTGATTGATGATCACTACTAGCCATCTGTCCATCAAGGAAAAGAGAGACCTCAAAGAGGAGATATTTCTTTGAATTCCAATTCAGTGGGTTGTC
AGGAGTTGACAGAGCAGCTTAGCATATGATTGCTCAATGAACGGTTTACTCATCCTACAATGCAGGATTCACTTCATCCTACAATGCAGGATTCACTTGAGTCAATTTTCCCAAAGGACACTCTTAAGAACTCACGATTTACCATCAACATTTTCACCTCTATTGGTCTTGGTGGTATCACTCAAAGTTTGCGTGAATGT
TTGAAGAATATGCAAAGACTTATATTGCAGCAGCTGAAGGCCATTTCTGAATCAGATGCCTCAATTTCTGGCGTTGATTCCTTTGCTTCTACTAAACATAGCTAGAAAAACTCTATACGACCACTTCACACAAATGAAATGATTAGGTTGACCTTGTTGACCCATGCTAATTGATGCATTGATAAAAGAATTTTTGCTCT
TCGCATTCATAGGATTACCATTTCATCCAACAAATATCTTATGGGAAATTTGTAATTCTGATTTCAGATATAAAACTTATTAATCTACG

Intron 2 GTCCCAGGAT ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.43	30	26	14
A2	46.05	41	35	14
A3	48.24	44	41	17
A4	34.38	21	11	17
A5	48.98	25	24	7
A6	49.03	79	76	21
A7	43.63	115	89	18
A8	41.83	89	64	15
A9	37.69	124	75	29
A10	50.75	99	102	21

>TRINITY_DN109430_c0_g4_i3 from sample A2 / Example spliced isoform

GTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAG
TTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGAGTCACAACCATGCCTATTCATAGTGTATACAAGGATGATAACGACGAACTTGAC
AGTGAAGGGCTCTTCAGGCTAGTAACTTGAAGACATGGGCTGGAAAAGTTGAGAATATCCCCTTGGCACATGATGCTTTCTTATCATGATGCCACTTTGGTCAAGTATGTGGGTGATTATTCCAAGGGAAATAGAGTTTTTGAGAGCTTTCATGTCAACAACTATAAATATTGATTGATGATCACTACTAGCCATCTGTC
CATCAAGGAAAAGAGAGACCTCAAAGAGGAGATATTTCTTTGAATTCCAATTCAGTGGGTTGTCAGGAGTTGACAGAGCAGCTTAGCATATGATTGCTCAATGAACGGTTTACTCATCCTACAATGCAGGATTCACTTCATCCTACAATGCAGGATTCACTTGAGTCAATTTTCCCAAAGGACACTCTTAAGAACTCACG
ATTTACCATCAACATTTTCACCTCTATTGGTCTTGGTGGTATCACTCAAAGTTTGCGTGAATGTTTGAAGAATATGCAAAGACTTATATTGCAGCAGCTGAAGGCCATTTCTGAATCAGATGCCTCAATTTCTGGCGTTGATTCCTTTGCTTCTACTAAACATAGCTAGAAAAACTCTATACGACCACTTCACACAAATG
AAATGATTAGGTTGACCTTGTTGACCCATGCTAATTG

>TRINITY_DN109430_c0_g4_i5 from sample A2 / Example unspliced isoform:

GTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGgtcccaggatgaacgaagcatcgaattaactgatggtcaaatcaaagctc
aagaagatttttcaacggcaatttccagacgttttgaggagttccattttttgaaactccctgcccaatccctctcacgcgtattcaagtagccctcgtcttttgttttctgcgaatttctcgaacctcatccaataatttgaatccgatgccttgatttgctagtggattgtgtgcttacgatggttctactttatgtt
tttatcatattttgcagGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCAT
TGGAGCTCATGGGTGAGAGACGAAGCCACCCAGTTTGCAATTAGTTCCTTTGTGGCATTGAAACCATAGTTGTCACATCTGAAATATAGCTCTTTATGTTAATAGCATCTTGCTTCTTTCGTGAAAATTGAGACTCTTTCTTCAAAAATTGTGC

Intron 3 GTTGCCTAAA ... ATTGCAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.4	27	37	42
A2	56	33	31	53
A3	59.06	35	57	44
A4	64.95	17	34	29
A5	58.33	15	21	21
A6	64.39	60	98	119
A7	63.95	83.4	141	155
A8	64.44	56	100	103
A9	68.39	55	112	126
A10	62.59	53.2	87.1	91

>TRINITY_DN109430_c0_g4_i3 from sample A2 / Example spliced isoform

GTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAG
TTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGAGTCACAACCATGCCTATTCATAGTGTATACAAGGATGATAACGACGAACTTGAC
AGTGAAGGGCTCTTCAGGCTAGTAACTTGAAGACATGGGCTGGAAAAGTTGAGAATATCCCCTTGGCACATGATGCTTTCTTATCATGATGCCACTTTGGTCAAGTATGTGGGTGATTATTCCAAGGGAAATAGAGTTTTTGAGAGCTTTCATGTCAACAACTATAAATATTGATTGATGATCACTACTAGCCATCTGTC
CATCAAGGAAAAGAGAGACCTCAAAGAGGAGATATTTCTTTGAATTCCAATTCAGTGGGTTGTCAGGAGTTGACAGAGCAGCTTAGCATATGATTGCTCAATGAACGGTTTACTCATCCTACAATGCAGGATTCACTTCATCCTACAATGCAGGATTCACTTGAGTCAATTTTCCCAAAGGACACTCTTAAGAACTCACG
ATTTACCATCAACATTTTCACCTCTATTGGTCTTGGTGGTATCACTCAAAGTTTGCGTGAATGTTTGAAGAATATGCAAAGACTTATATTGCAGCAGCTGAAGGCCATTTCTGAATCAGATGCCTCAATTTCTGGCGTTGATTCCTTTGCTTCTACTAAACATAGCTAGAAAAACTCTATACGACCACTTCACACAAATG
AAATGATTAGGTTGACCTTGTTGACCCATGCTAATTG

>TRINITY_DN109430_c0_g4_i4 from sample A2 / Example unspliced isoform:

GTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTCCCAGGATGAACGAAGCATCGAATTAACTGATGGTCAAATCAAAGCTC
AAGAAGATTTTTCAACGGCAATTTCCAGACGTTTTGAGGAGTTCCATTTTTTGAAACTCCCTGCCCAATCCCTCTCACGCGTATTCAAGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCT
TGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGAGgttgcctaaatgttcagtgaaaataagcgggaatataacaaaagagttcatcaaattgcggagcagagttggattgcaaaagTCACAACCATGCCTATTCATAGTGTATACAAGG
ATGATAACGACGAACTTGACAGTGAAGGGCTCTTCAGGCTAGTAACTTGAAGACATGGGCTGGAAAAGTTGAGAATATCCCCTTGGCACATGATGCTTTCTTATCATGATGCCACTTTGGTCAAGTATGTGGGTGATTATTCCAAGGGAAATAGAGTTTTTGAGAGCTTTCATGTCAACAACTATAAATATTGATTGATG
ATCACTACTAGCCATCTGTCCATCAAGGAAAAGAGAGACCTCAAAGAGGAGATATTTCTTTGAATTCCAATTCAGTGGGTTGTCAGGAGTTGACAGAGCAGCTTAGCATATGATTGCTCAATGAACGGTTTACTCATCCTACAATGCAGGATTCACTTCATCCTACAATGCAGGATTCACTTGAGTCAATTTTCCCAAAG
GACACTCTTAAGAACTCACGATTTACCATCAACATTTTCACCTCTATTGGTCTTGGTGGTATCACTCAAAGTTTGCGTGAATGTTTGAAGAATATGCAAAGACTTATATTGCAGCAGCTGAAGGCCATTTCTGAATCAGATGCCTCAATTTCTGGCGTTGATTCCTTTGCTTCTACTAAACATAGCTAGAAAAACTCTAT
ACGACCACTTCACACAAATGAAATGATTAGGTTGACCTTGTTGACCCATGCTAATTG

Intron 4 GTACGGCCTC ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.82	30	14	14
A2	27.43	41	17	14
A3	35.77	44	32	17
A4	33.33	21	4	17
A5	29.58	25	14	7
A6	26.17	79	35	21
A7	18.44	115	34	18
A8	20.89	89	32	15
A9	21.27	124	38	29
A10	23.85	99	41	21

>TRINITY_DN108417_c0_g4_i7 from sample A3 / Example spliced isoform

TCGCCGTGTTTCACAAGTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTTGACTGTATTTTTCTGAAATGGAGATGAAACG
GAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGGAGTTGACAGAGCAGCTTAGCATATGATTGCTCAATGAA
CGGTTTACTCATCCTACAATGCAGGATTCACTTCATCCTACAATGCAGGATTCACTTGAGTCAATTTTCCCAAAGGACACTCTTAAGAACTCACGATTTACCATCAACATTTTCACCTCTATTGGTCTTGGTGGTATCACTCAAAGTTTGCGTGAATGTTTGAAGAATATGCAAAGACTTATATTGCAGCAGCTGAAGGC
CATTTCTGAATCAGATGCCTCAATTTCTGGCGTTGATTCCTTTGCTTCTACTAAACATAGCTAGAAAAACTCTATACGACCACTTCACACAAATGAAATGATTAGGTTGACCTTGTTGACCCATGCTAATTGATGCATTGATAAAAGAATTTTTGCTCTTCGCATTCATAGGATTACCATTTCATCCAACAAATATCTTA
TGGGAAATTTGTAATTCTGATTTCAGATATAAAACTTATTAATCTAC

>TRINITY_DN108417_c0_g4_i1 from sample A3 / Example unspliced isoform:

TCGCCGTGTTTCACAAGTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGgtacggcctcacaaatttcgtctgtatggccgtt
gaaggccagctttggttgtcatgctctgctttggatgaaatccaatttgaagcttttgacaggttttaggctcgtatgtttgagcaataagcagcgtcactaaaaattattcagatataaaaaaaatgtacttcgagttggataatgccaactcttataaaaatgaatctgttgaacaccaaagattcgtccaggtccca
ggatgaacgaagcatcgaattaactgatggtcaaatcaaagctcaagaagatttttcaacggcaatttccagacgttttgaggagttccattttttgaaactccctgcccaatccctctcacgcgtattcaagtagccctcgtcttttgttttctgcgaatttctcgaacctcatccaataatttgaatccgatgccttg
atttgctagtggattgtgtgcttacgatggttctactttatgtttttatcatattttgcagGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGT
TGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGGTGAGAGACGAAGTCCCCCAATTTGTAGTTGTAGTTCAAGTTATTTTATGGCATTGAAACTATAGTTGTCACATCTGAGATTTCACTCTTTATGTTAATAACATTTCTTTCGTGAAAATTGAACCTCTTTATTTAAAAAAATGT
CCCAAATGGATCTTTCATCAACAACATGTGCTCTATCTGAACTTGAAAGGAAATTTTGTCTAATTCCTTGTTTGTCTAGGAAAGATGCCTTCTCCACACCAAGATTTAATCAAATCCACAAATGTTTTGTAACCCCTAACCATACAATGGTCATGCTCGCTTACCGCTTTGCATCTATTGGTCCTCTCGACCAATCTGCT
CATCTTGATCCAACTGTTCCTCCGGCGGTGGCTGCAGCAATAGGAAAGGAAGAGGATAGTTGGCATCCTAATCATATTCATGATGATGATGCTGCTCCTACAGCAATATAGGAGGAGGAAAGGCAGGATGATCAAATCATATCGCCTGCTACCACTCACGCAGACATTCATACCCAGCACGCTGATATTTATTCAACTCC
AACTGATGCTTTGCATGTATCTGCTGCAATAGACAATAGCCAAGAGCCATGCTCACAATGACGAGG

Intron 5 GTATTCAAGT ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.38	21	9	14
A2	37.14	22	12	14
A3	41.38	17	7	17
A4	46.67	12	4	17
A5	37.5	10	5	7
A6	28.46	44	14	21
A7	30.97	39	17	18
A8	23.89	43	12	15
A9	25.41	69	18	29
A10	27.59	42	11	21

>TRINITY_DN39576_c2_g2_i7 from sample A8 / Example spliced isoform

CCGTGTTTCACAAGTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTCCCAGGATGAACGAAGCATCGAATTAACTGATGGT
CAAATCAAAGCTCAAGAAGATTTTTCAACGGCAATTTCCAGACGTTTTGAGGAGTTCCATTTTTTGAAACTCCCTGCCCAATCCCTCTCACGCGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTA
CATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGAGGTTGCCTAAATGTTCAGTGAAAATAAGCGGGAATATAACAAAAGAGTTCATCAAATTGCGGAGCAGAGTTGGATTGCAAAAGTCACAACCATGCCTATTCATAGTGTATA
CAAGGATGATAACGACGAACTTGACAGTGAAGGGCTCTTCAGGCTAGTAACTTGAAGACATGGGCTGGAAAAGTTGAGAATATCCCCTTGGCACATGATGCTTTCTTATCATGATGCCACTTTGGTCAAGTATGTGGGTGATTATTCCAAGGGAAATAGAGTTTTTGAGAGCTTTCATGTCAACAACTATAAATATTGAT
TGATGATCACTACTAGCCATCTGTCCATCAAGGAAAAGAGAGACCTCAAAGAGGAGATATTTCTTTGAATTCCAATTCAGTGGGTTGTCAGGAGTTGACAGAGCAGCTTAGCATATGATTGCTCAATGAACGGTTTACTCATCCTACAATGCAGGATTCACTTCATCCTACAATGCAGGATTCACTTGAGTCAATTTTCC
CAAAGGACACTCTTAAGAACTCACGATTTACCATCAACATTTTCACCTCTATTGGTCTTGGTGGTATCACTCAAAGTTTGCGTGAATGTTTGAAGAATATGCAAAGACTTATATTGCAGCAGCTGAAGGCCATTTCTGAATCAGATGCCTCAATTTCTGGCGTTGATTCCTTTGCTTCTACTAAACATAGCTAGAAAAAC
TCTATACGACCACTTCACACAAATGAAATGATTAGGTTGACCTTGTTGACCCATGCTAATTGATGCATTGATAAAAGAATTTTTGCTCTTCGCATTCATAGGATTACCATTTCATCCAACAAATATCTTATGGGAAATTTGTAATTCTGATTTCAGATATAAAACTTATTAATCTACCAACATTTTCTGAGAGATTCCCA
TAATTTTTTATAGTTAATGCTATGTTAAATGTTTTGTACAACATGTTATATTTGACATTTTGTACAAGCTAATAGTAAATGCT

>TRINITY_DN39576_c2_g2_i6 from sample A8 / Example unspliced isoform:

CCGTGTTTCACAAGTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTCCCAGGATGAACGAAGCATCGAATTAACTGATGGT
CAAATCAAAGCTCAAGAAGATTTTTCAACGGCAATTTCCAGACGTTTTGAGGAGTTCCATTTTTTGAAACTCCCTGCCCAATCCCTCTCACGCgtattcaagtagccctcgtcttttgttttctgcgaatttctcgaacctcatccaataatttgaatccgatgccttgatttgctagtggattgtgtgcttacgatggt
tctactttatgtttttatcatattttgcagGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAG
TTTGTGCAACCATTGGAGCTCATGG

Intron 6 GTAGCCCTCG ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.57	20	8	10.8
A2	35.29	22	12	10.8
A3	27.27	16	6	13.5
A4	23.53	13	4	12.8
A5	30.77	9	4	5.8
A6	22.22	42	12	15.8
A7	26	37	13	13.8
A8	20.75	42	11	11.3
A9	17.72	65	14	21.8
A10	20.41	39	10	15.8

>TRINITY_DN109430_c0_g4_i4 from sample A2 / Example spliced isoform

GTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTCCCAGGATGAACGAAGCATCGAATTAACTGATGGTCAAATCAAAGCTC
AAGAAGATTTTTCAACGGCAATTTCCAGACGTTTTGAGGAGTTCCATTTTTTGAAACTCCCTGCCCAATCCCTCTCACGCGTATTCAAGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCT
TGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGAGGTTGCCTAAATGTTCAGTGAAAATAAGCGGGAATATAACAAAAGAGTTCATCAAATTGCGGAGCAGAGTTGGATTGCAAAAGTCACAACCATGCCTATTCATAGTGTATACAAGG
ATGATAACGACGAACTTGACAGTGAAGGGCTCTTCAGGCTAGTAACTTGAAGACATGGGCTGGAAAAGTTGAGAATATCCCCTTGGCACATGATGCTTTCTTATCATGATGCCACTTTGGTCAAGTATGTGGGTGATTATTCCAAGGGAAATAGAGTTTTTGAGAGCTTTCATGTCAACAACTATAAATATTGATTGATG
ATCACTACTAGCCATCTGTCCATCAAGGAAAAGAGAGACCTCAAAGAGGAGATATTTCTTTGAATTCCAATTCAGTGGGTTGTCAGGAGTTGACAGAGCAGCTTAGCATATGATTGCTCAATGAACGGTTTACTCATCCTACAATGCAGGATTCACTTCATCCTACAATGCAGGATTCACTTGAGTCAATTTTCCCAAAG
GACACTCTTAAGAACTCACGATTTACCATCAACATTTTCACCTCTATTGGTCTTGGTGGTATCACTCAAAGTTTGCGTGAATGTTTGAAGAATATGCAAAGACTTATATTGCAGCAGCTGAAGGCCATTTCTGAATCAGATGCCTCAATTTCTGGCGTTGATTCCTTTGCTTCTACTAAACATAGCTAGAAAAACTCTAT
ACGACCACTTCACACAAATGAAATGATTAGGTTGACCTTGTTGACCCATGCTAATTG

>TRINITY_DN109430_c0_g4_i5 from sample A2 / Example unspliced isoform:

GTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTCCCAGGATGAACGAAGCATCGAATTAACTGATGGTCAAATCAAAGCTC
AAGAAGATTTTTCAACGGCAATTTCCAGACGTTTTGAGGAGTTCCATTTTTTGAAACTCCCTGCCCAATCCCTCTCACGCGTATTCAAgtagccctcgtcttttgttttctgcgaatttctcgaacctcatccaataatttgaatccgatgccttgatttgctagtggattgtgtgcttacgatggttctactttatgtt
tttatcatattttgcagGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCAT
TGGAGCTCATGGGTGAGAGACGAAGCCACCCAGTTTGCAATTAGTTCCTTTGTGGCATTGAAACCATAGTTGTCACATCTGAAATATAGCTCTTTATGTTAATAGCATCTTGCTTCTTTCGTGAAAATTGAGACTCTTTCTTCAAAAATTGTGC

Intron 7 GTACGGCCTC ... ATTCGTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.47	26	14	11
A2	23.91	35	17	5
A3	36.92	41	32	16
A4	43.59	11	4	13
A5	32.39	24	14	9
A6	24.38	76	35	14
A7	19.82	89	34	10
A8	22.89	64	32	6
A9	23.08	75	38	7
A10	24.16	102	41	24

>TRINITY_DN108417_c0_g4_i5 from sample A3 / Example spliced isoform

TCGCCGTGTTTCACAAGTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGGTCCCAGGATGAACGAAGCATCGAATTAACTGAT
GGTCAAATCAAAGCTCAAGAAGATTTTTCAACGGCAATTTCCAGACGTTTTGAGGAGTTCCATTTTTTGAAACTCCCTGCCCAATCCCTCTCACGCGTATTCAAGTAGCCCTCGTCTTTTGTTTTCTGCGAATTTCTCGAACCTCATCCAATAATTTGAATCCGATGCCTTGATTTGCTAGTGGATTGTGTGCTTACGAT
GGTTCTACTTTATGTTTTTATCATATTTTGCAGGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGTTGTCCCGATTGCTGTTGAAATGGAGCTT
TAGTTTGTGCAACCATTGGAGCTCATGGGTGAGAGACGAAGTCCCCCAATTTGTAGTTGTAGTTCAAGTTATTTTATGGCATTGAAACTATAGTTGTCACATCTGAGATTTCACTCTTTATGTTAATAACATTTCTTTCGTGAAAATTGAACCTCTTTATTTAAAAAAATGTCCCAAATGGATCTTTCATCAACAACATG
TGCTCTATCTGAACTTGAAAGGAAATTTTGTCTAATTCCTTGTTTGTCTAGGAAAGATGCCTTCTCCACACCAAGATTTAATCAAATCCACAAATGTTTTGTAACCCCTAACCATACAATGGTCATGCTCGCTTACCGCTTTGCATCTATTGGTCCTCTCGACCAATCTGCTCATCTTGATCCAACTGTTCCTCCGGCGG
TGGCTGCAGCAATAGGAAAGGAAGAGGATAGTTGGCATCCTAATCATATTCATGATGATGATGCTGCTCCTACAGCAATATAGGAGGAGGAAAGGCAGGATGATCAAATCATATCGCCTGCTACCACTCACGCAGACATTCATACCCAGCACGCTGATATTTATTCAACTCCAACTGATGCTTTGCATGTATCTGCTGCA
ATAGACAATAGCCAAGAGCCATGCTCACAATGACGAGG

>TRINITY_DN108417_c0_g4_i1 from sample A3 / Example unspliced isoform:

TCGCCGTGTTTCACAAGTTTCATTTACAAGCGCCAGCCTGCAATATTTGTCTATGGCGATGAGATGATGGAAGCGGTGGAAGCGCTCCGAAGGCATTTTTCGAAGTCTTTGGCCCCCAAAGCTAGACCTAGAGCCTGATTCCACCCATGCCTTGCGATCGAAACAGgtacggcctcacaaatttcgtctgtatggccgtt
gaaggccagctttggttgtcatgctctgctttggatgaaatccaatttgaagcttttgacaggttttaggctcgtatgtttgagcaataagcagcgtcactaaaaattattcagatataaaaaaaatgtacttcgagttggataatgccaactcttataaaaatgaatctgttgaacaccaaagattcgtccagGTCCCA

GGATGAACGAAGCATCGAATTAACTGATGGTCAAATCAAAGCTCAAGAAGATTTTTCAACGGCAATTTCCAGACGTTTTGAGGAGTTCCATTTTTTGAAACTCCCTGCCCAATCCCTCTCACGCGTATTCAAGTAGCCCTCGTCTTTTGTTTTCTGCGAATTTCTCGAACCTCATCCAATAATTTGAATCCGATGCCTTG
ATTTGCTAGTGGATTGTGTGCTTACGATGGTTCTACTTTATGTTTTTATCATATTTTGCAGGTTGACTGTATTTTTCTGAAATGGAGATGAAACGGAGATGGGCCTTTGAGTTGAATATGCAGCGTATTTTCTCGGTTTGTAAACTTGACGGATGAGGCACATCATTACATCTTGCAGCCCGAATGAGACATGCAAGTGT
TGTCCCGATTGCTGTTGAAATGGAGCTTTAGTTTGTGCAACCATTGGAGCTCATGGGTGAGAGACGAAGTCCCCCAATTTGTAGTTGTAGTTCAAGTTATTTTATGGCATTGAAACTATAGTTGTCACATCTGAGATTTCACTCTTTATGTTAATAACATTTCTTTCGTGAAAATTGAACCTCTTTATTTAAAAAAATGT
CCCAAATGGATCTTTCATCAACAACATGTGCTCTATCTGAACTTGAAAGGAAATTTTGTCTAATTCCTTGTTTGTCTAGGAAAGATGCCTTCTCCACACCAAGATTTAATCAAATCCACAAATGTTTTGTAACCCCTAACCATACAATGGTCATGCTCGCTTACCGCTTTGCATCTATTGGTCCTCTCGACCAATCTGCT
CATCTTGATCCAACTGTTCCTCCGGCGGTGGCTGCAGCAATAGGAAAGGAAGAGGATAGTTGGCATCCTAATCATATTCATGATGATGATGCTGCTCCTACAGCAATATAGGAGGAGGAAAGGCAGGATGATCAAATCATATCGCCTGCTACCACTCACGCAGACATTCATACCCAGCACGCTGATATTTATTCAACTCC
AACTGATGCTTTGCATGTATCTGCTGCAATAGACAATAGCCAAGAGCCATGCTCACAATGACGAGG