sp|P37853|CHLB_PICMA ----> Light-independent protochlorophyllide reductase subunit B (Fragment)

Intron 1 GTAAGTGCTT ... CCATTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.61	13	20	4
A2	50	15	15	1
A3	52.94	16	18	1
A4	57.14	24	32	6
A5	50	8	8	1
A6	53.41	82	94	14
A7	52.74	69	77	2
A8	56.32	38	49	4
A9	55.4	62	77	3
A10	55.06	40	49	1

>TRINITY_DN42928_c0_g1_i7 from sample A6 / Example spliced isoform

AAATTAGCGTTGGTAAAACACCGTTGGTGAAATTGTGTCGAGAGCGCACCCACGATTTACACCGTTGGTGATTTGGCTTCTGACCATAGTGAATCTCACTGAATTAATCATCGGCCTTTCGAGCGAACCCTTCAATTATCTTTACGGCACAAGTTTTTGATTTCAAATCTCCCTCTTCGGAACAGTCTTGTAAAATCCAT
GTTCCGAGCTTCATCTTCTGTCAAGTAGATTTCGTGAAATTTGAGGATCCAGTCAGTGTATGCTTTGAAACGTTGAAAGAAGCAGGAACCCAATTCAGAGATCTGCTGTGGCAGGGCAGGGGTGATTACATTTTTAGGGTGATTACATTTTTGTGATGCGCAGACAGGTGGTGCCAAAGACCGAATCACTGCTCACAAGA
GCAGTGAACAAGGTCTTTGCGTTTGTTCGCCTTGCAGAGTTTGAAATTCTTTTTGTACTCTTCATACTGATTGTGTACATTATTTTCAAAGACATTTGGAAAATTAAAGCCAAAGATGGACTATACCTGAATTAGGCCCACTTTACACTGTTAGATTGATCAGCCTAAACTTATTGACAGACTGACATCTCCATAAGTTT
CTGGTTTCGTACTGATCCCTTAAAATTGGTTTGATCTGTTAGGAAAAGTTTGAATAAAAATAAACTGAATTTTGCGAATAGTCGAGGAGGATGATATGTCATGGTTAAAGCCAGTTTGTGGATTTTACTAACTCATCGTGCCAGAATATAACATATTTGAATAATTCAAGAATAATGATAGTGTAAAATAGCCATTATAG
TGGTAATGTTTTATGATTATCTGAGCATACGGCTGTCATTAATTTCAGTTCATGATGTGGAATTAAATTGAACCGAGCTTTTGGTAAATTTTCAGAATCTCAATTGATCTCCTTTGGGAATTATTCAATTACTTTCATTGTTTAGGAATTTTTAATAACTGTCTCATGTCTAAACTCACAATTGTCTTGTTGAATATAAA
AGCCAAAGTTGTCACACTTGAACTCGGCTGGCCTGGTACTCGGCAAGACTTGGTCACTCGGGAAAGACTCGGAAAATACAAGAAAGGAAAGTCAAAATATAGTCCATGCAAGTCTTGGCTAGTCTGTCTGAGTCTTGGTGAGTGGCGAGTTCAGGGCTGAGTATGAG

>TRINITY_DN42928_c0_g1_i3 from sample A6 / Example unspliced isoform:

AAATTAGCGTTGGTAAAACACCGTTGGTGAAATTGTGTCGAGAGCGCACCCACGATTTACACCGTTGGTGATTTGGCTTCTGACCATAATGAATCTCACTGAGTTAATCATCGGCCTTTCGAGCGAACCCTTCAATTATCTTTACGGCACAAGTTTTTGATTTCAAATCTCCTTCTTCGGAACAGTTTTGTAAAATCCAT
GTTCCGAACTTCATCTTCTGTCAAGTAGATTTCGTGAAATTTGAGGATCCAGTCAGTGTATGCTTTGAAACGTTGAAAGAAGCAGGAACCAAATTCAGAGGGGTGATTACATTTTTGTGATGCGCAGACAGGTGGTGCCAAAGACCGAATCACTGCTCACAAGAGCAGTGAACAAGGTCTTTGCGTTTGTTCGCCTTGCA
GAGTTTGAAATTCTTTTTGTACTCTTCATACTGATTGTGTACATTATTTTCAAAGACATTgtaagtgcttcaatctcttctgctctgcccattatatatattttttaatacaaataaaaggtcattcccaaggttcagatagtttgttctttctatccctgcaatttccaagcatttacatcgtttttcttttatcttat
taacataatttgccaaccagagaggttaatttttgtggagtcttttgtccattggcagTGGAAAATTAAAGCCAAAGATGGACTATACCTGAATTAGGCCCACTTTACACTGTTAGATTGATCAGCCTAAACTTATTGACAGACTGACATCTCCATAAGTTTCTGGTTTCGTACTGATCCCTTAAAATTGGTTTGATCTG
TTAGGAAAAGTTTGAATAAAAATAAACTGAATTTTGCGAATAGTCGAGGAGGATGATATGTCATGGTTAAAGCCAGTTTGTGGATTTTACTAACTCATCGTGCCAGAATATAACATATTTGAATAATTCAAGAATAATGATAGTGTAAAATAGCCATTATAGTGGTAATGTTTTATGATTATCTGAGCATACGGCTGTCA
TTAATTTCAGTTCATGATGTGGAATTAAATTGAACCGAGCTTTTGGTAAATTTTCAGAATCTCAATTGATCTCCTTTGGGAATTATTCAATTACTTTCATTGTTTAGGAATTTTTAATAACTGTCTCATGTCTAAACTCACAATTGTCTTGTTGAATATAAAAGCCAAAGTTGTCACACTTGAACTCGGCTGGCCTGGTA
CTCGGCAAGACTTGGTCACTCGGGAAAGACTCGGAAAATACAAGAAAGGAAAGTCAAAATATAGTCCATGCAAGTCTTGGCTAGTCTGTCTGAGTCTTGGTGAGTGGCGAGTTCAGGGCTGAGTATGAG

Intron 2 GTATGCCTCT ... CAATGGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	8	7	10
A2	50	5	1	5
A3	64.29	5	1	9
A4	55.56	4	0	5
A5	51.85	13	2	14
A6	51.61	60	20	64
A7	51.92	25	6	27
A8	54.84	14	3	17
A9	48.44	33	9	31
A10	55.22	30	13	37

>TRINITY_DN45022_c0_g1_i4 from sample A10 / Example spliced isoform

CGTTGGTAAAACACCGTTGGTGAAATTGTGTCGAGAGCGCACCCACGATTTACACCGTTGGTGATTTGGCTTCTGACCATAATGAATCTCACTGAGTTAATCATCGGCCTGTCGAGCGAACCCTTCAATTATCTTTACGGCACAAGTTTTTGATTTCAAATCTCCTTCTTCGGAACAGTTTTGTAAAATCCATGTTCCGA
ACTTCATCTTCTGTCAAGTAGATTTCGTGAAATTTGAGGATCCAGTCAGTGTATGCTTTGAAACGTTGAAAGAAGCAGGAACCAAATTCAGAGGAATAGCTTGCAATGGTATGGAGAAAATTGGGAGTCTTATGGGTGGCATGTCAAATCTTCTTCGGGGTTCAAAAAGGGCTATATTGTGTGGTTTTCTAACCTGCACG
TGTTACTCAATGATACTCGGATGATTGCAGAGGCCACGGATTATGTCCTGCCTTGGCGGGGTGATTTTTCATCTTTTTTGCCTGAGGGTGATTACATTTTTGTGATGCGCAGACAGGTGGTGCCAAAGACCGAATCACTGCTCACAAGAGCAGTGAACAAGGTCTTTGCGTTTGTTCGCCTTGCAGAGTTTGAAATTCTT
TTTGTACTCTTCATACTGATTGTGTACATTATTTTCAAAGACATTACATCCACGCCTGAGTACAATCAGTTTCTTGTGAAACGTTTTCCACGTTAAAAGATGTATATAAATGTATTTTAGTCAGGCTTCCCATCTATTCTTTACAATATCAGATGAGATTACTTGCAATAAATTCCATGACATGTTGAGGTTGGCTTCTT
GATATTATGGTGAGCTACAAATGATGCAGCTTTTTAAACTGTGTGATCTTATCCCTTCTTTCTGATTTTAGCACATTCAAATTCTCACTGTTCAGCTCTAAGAGGAATAAGGATAAATAAATCTATTTTGTGGTATAGAACTCCTTTTTTACAGACAATACGGAAGTATATTGTTCCGGGGATTTTCTTTAGTTTCTATT
TTGAGAATGGAACTTTTGCCATTTTGTCCATTTTACGGGACAAAACCCCTTGTAGATATGGGAAGAGATGCATATATAGGGCATAAATTTGTAATGGATTACAATTTTATGTTAATGATGTTAAGATTCAAGCTACTTTCAATAAAAGCTTGAATTTGTACATTTTCTACAAGAGCAACTATTTTG

>TRINITY_DN45022_c0_g1_i1 from sample A10 / Example unspliced isoform:

AAATTCCCCAAAGGCAAATCTCTGTTTTGAAATTTCACGACAGATGGATTTTTTCTTATGTTAATAATGCCCTGAAATAATGGGCCTATGGTTTTCGAGAATACCCATTTGTATATACAGTTCCGCCTTATGTTATGGCATATTGAGTGTTTGAATATTACCCTGGCAGTAAAATCCATGTTCCGAACTTCATCTTCTGT
CAAGTAGATTTCGTGAAATTTGAGGATCCAGTCAGTGTATGCTTTGAAACGTTGAAAGAAGCAGGAACCAAATTCAGAGGAATAGCTTGCAATGGTATGGAGAAAATTGGGAGTCTTATGGGTGGCATGTCAAATCTTCTTCGGGGTTCAAAAAGGGCTATATTGTGTGGTTTTCTAACCTGCACGTGTTACTCAATGAT
ACTCGGATGATTGCAGAGGCCACGGATTATGTCCTGCCTTGGCGGGGTGATTTTTCATCTTTTTTGCCTGAgtatgcctcttgacaatgggcagGGGTGATTACATTTTTGTGATGCGCAGACAGGTGGTGCCAAAGACCGAATCACTGCTCACAAGAGCAGTGAACAAGGTCTTTGCGTTTGTTCGCCTTGCAGAGTTT
GAAATTCTTTTTGTACTCTTCATACTGATTGTGTACATTATTTTCAAAGACATTGTAAGTGCTTCAATCTCTTCTGCTCTGCCCATTATATATATTTTTTAATACAAATAAAAGGTCATTCCCAC