sp|Q9FF55|PDI14_ARATH ----> Protein disulfide isomerase-like 1-4

Intron 1 GTATGTTTGG ... TTCCTGGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.81	23	0.3	4
A2	13.89	31	0	5
A3	16.67	25	0	5
A4	39.29	17	1	11
A5	0	25	0	0
A6	25.9	103	2	36
A7	18.12	113	0	25
A8	22.56	127	0	37
A9	22.11	148	2.7	42
A10	13.16	66	0	10

>TRINITY_DN101566_c0_g1_i3 from sample A4 / Example spliced isoform

CACGTTCGTGTGATTTCTCTGCTCGTTACAGTGTCACACACAGTCTTACAAACCCTCGGTCTCTCGAGTTCGTGCAGCCATGGCGGCCGCGAAGCTTGTGATCTTCGGTCTCGTGGCGCTTCTGTTGTCTGTCAGTTTCCAGCACCTTCAGGCCGTCGAAAATGACAGCGAAATCGACGAGATCGAAGAGTTGGAGCTCC
TCGAAGACGAGGGGGAAGAAAATACGACAATGGATCCTTTCACGGCCGGCGAGGAACCAGAGCTGGAAGAAGATTTTGAAGAAGGATCGTACGACCTGGAAGGTGAAGATGCCGATACGGATGCGCCGGCCGTCGACGAGAAAGATGTCGCGGTGTTAAAGGAGAGCAACTTCAGCGATGTCGTGAGCAAAAACCGGTAT
GTGCTGGTCGAGTTTTATGCGCCCTGGTGCGGCCATTGCCAGCGCTTGGTTCCGGAGTATGCTGCGGCTGCGACTGAGTTGAAGGGGGAGGTCGTTCTGGCTAAAGTGGATGCCACTGAAGAAAATGATCTGGCGCAAAAATTCGAAGTTCAGGGGTTTCCGACCATCTTGTTTTTCATCGATGGAGTTCACAAGCAGTA
CCCCGGCCAAAGAACAAAAGAAGCAATTGTTAATTGGATCAAGCGTAAAACTGGCCCTGCCGTATCCAACCTCACCACAACTGAGGATGCAGAGACTCTACTAGACTCAGGAACCACAGCTGCTGTTGGTTTGTTTGACTCATTAGAGGGCATTGAAAATGAGGAGTTTGAAGCAGCTTCCAGGCAAGAAGATGATGTGT
TATTTTATCAAACAACTAGTGAACGTGTAGCTGCTCTTCTCGACATTGACACAAAGGAAAAGCGGCCAGCATTGGTACTATTGAAGAAGGAGCCTGAAAAAATATCCCATTTTGATGGAAAATTTGAAAAAGCTGCCATATCTGAGTTCATCTTTGCAAACAAGCTCCCATTAGTGACGACCTTCACCAGGGAAAGTGCT
AATCTGATATTTGACAGTTCTATAAAGAAACAAATCCTGCTCTTTGTATCTGCCAAGGATTATGAAAAATTCATTCCATCATTTCAAGAAGCTGCAAAATCATTCAAGGGAAAGATCGTTTTTGTGCATGTTGATTCTGACAATGCTGATGTTGGGAAACCAATTATGGAATTCTTCGGCTTAAGTGGAGAAGAACCAAA
GGTAATTGCATGCCTTTTAACTGAAGAACCCATCAAATATATGTTTGAAGCTGAAATAAATGCTGATAACATCAAGGCTTTTGGAGAGGACTTCCTTGCAGACAAACTCAAGCCTTTCTTCAAGTCCGACCCAGTGCCGGAAAAGAATGACGGAGATGTGAAAATTGTTGTGGGAAATAATTTTGATGAGATTGTACTGG
ATGAATCAAAGGATGTCCTTCTCGAGTTATATGCACCTTGGTGTGGGCATTGCCAAGCATTGGAACCTATATACGATAAACTTGCAAAGCAGCTTCGTGGGGTTGACTCCCTTGTCATAGCTAAGATGGATGGTACAAGTAACGAGCATGCTAGGGCCAAGTCTGATGGGTATCCCACGATTCTATTCTATCCAGCAGGA
AACAAGAGTTTTGATCCTATTACTTTTGATGGTGATCGCACTGTCAAAGGATTTTACAAATTCTTAAAGACAAATACAGCTATCCCATTCAAGTTGCCAAAGAAGAGCAAGACTGAAAGTGTAGAGGCTACTCCAGCTACTCAAGATACCAGTGCTGCTGACCAACCTAAAGATGAACTATGATGCTGAGTATTGATATT
TGTGTACTTAGAATCGAAATTTTCCTCAATTTTCCCAAATTTTATCTCTATGAGGGCAATAAGCCAGTAATAAAGTTTTATAATTTATGAAAACCCTAAAAGCGCCAAGCAATTACACAATAACATTTCGGATTTAGTTGTAGTTTGATTTATCGCAAGATAAAATTGATTTCTTCAAATCCTGTTTGCCTACTTGTCAC
GTTTACATCCAAGATATTAAAATTAATGGCCTGGTTCTATTAATAATTAACCATTTAAACACCTTCATCACTGAGGTGAGGTTTTTTCCAGGTATTAGAAGGGTTGTTAGTTATCCTTAAATTTGATATTCCGACTGATGGATATATATTGATGGTATTCACGAATATAAATACCTGTTGCATAATCTTCCATCTCGGAA
AGTATTTTCCTTTCCATTATTTAGTTCAATCTAAGGATTTTTGCAGCTTATATGTTGCCTTTCCATATGCAGAGACAAATGCCCATGTTCACTATTTATCAGTAAGAATTAAAATGTCTGTGTTGCAGTCTTTTGTGTTCTGTTGCATTTATTAACATTTGAGTCTCGAGC

>TRINITY_DN101566_c0_g1_i2 from sample A4 / Example unspliced isoform:

CAATGAGTTACCTGTTAGCCAATCACTAAATTTTCCAGGCCATAACGAGTCACACCTGTGTTTTGAATTTTGATAGAATGACGGAGATGTGAAAATTGTTGTGGGAAATAATTTTGATGAGATTGTACTGGATGAATCAAAGGATGTCCTTCTCGAGgtaagtttggtgtgcttaaaaattagaaggaactttcatatgg
tttttgcagaagtctgtttaatttcttagtttgacaaacttttgttcctggtagTTATATGCACCTTGGTGTGGGCATTGCCAAGCATTGGAACCTATATACGATAAACTTGCAAAGCAGCTTCGTGGGGTTGACTCCCTTGTCATAGCTAAGATGGATGGTACAAGTAACGAGCATGCTAGGGCCAAGTCTGATGGGTA
TCCCACGATTCTATTCTATCCAGCAGGAAACAAGAGTTTTGATCCTATTACTTTTGATGGTGATCGCACTGTCAAAGGATTTTACAAATTCTTAAAGACAAATACAGCTATCCCATTCAAGTTGCCAAAGAAGAGCAAGACTGAAAGTGTAGAGGCTACTCCAGCTACTCAAGATACCAGTGCTGCTGACCAACCTAAAG
ATGAACTATGATGCTGAGTATTGATATTTGTGTACTTAGAATCGAAATTTTCCTCAATTTTCCCAAATTTTATCTCTATGAGGGCAATAAGCCAGTAATAAAGTTTTATAATTTATGAAAACCCTAAAAGCGCCAAGCAATTACACAATAACATTTCGGATTTAGTTGTAGTTTGATTTATCGCAAGATAAAATTGATTT
CTTCAAATCCTGTTTGCCTACTTGTCACGTTTACATCCAAGATATTAAAATTAATGGCCTGGTTCTATTAATAATTAACCATTTAAACACCTTCATCACTGAGGTGAGGTTTTTTCCAGGTATTAGAAGGGTTGTTAGTTATCCTTAAATTTGATATTCCGACTGATGGATATATATTGATGGTATTCACGAATATAAAT
ACCTGTTGCATAATCTTCCATCTCGGAAAGTATTTTCCTTTCCATTATTTAGTTCAATCTAAGGATTTTTGCAGCTTATATGTTGCCTTTCCATATGCAGAGACAAATGCCCATGTTCACTATTTATCAGTAAGAATTAAAATGTCTGTGTTGCAGTCTTTTGTGTTCTGTTGCATTTATTAACATTTGAGTCTCGAGC

Intron 2 GTTTTTGTTT ... ATTTTGATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.9	30	14	6.5
A2	19.64	27	7	8
A3	15.79	26	8	6
A4	13.34	22	5	4
A5	16.28	29	4	7
A6	11.73	75	10	11.5
A7	12.96	80	9.5	14
A8	10.6	93	14	12.5
A9	12.14	106	11.5	17
A10	10.91	43	6.5	6

>TRINITY_DN110190_c0_g3_i1 from sample A2 / Example spliced isoform

GCTCGTTACAGTGTCACACACAGTCTTACAAACCCTCGGTCTCTCGAGTTCGTGCAGCCATGGCGGCCGCGAAGCTTGTGATCTTCGGTCTCGTGGCGCTTCTGTTGTCTGTCAGTTTCCAGCACCTTCAGGCCGTCGAAAATGACAGCGAAATCGACGAGATCGAAGAGTTGGAGCTCCTCGAAGACGAGGGGGAAGAA
AATACGACAATGGATCCTTTCACGGCCGGCGAGGAACCAGAGCTGGAAGAAGATTTTGAAGAAGGATCGTACGACCTGGAAGGTGAAGATGCCGATACCGATGCGCCGGCCGTCGACGAGAAAGATGTCGCGGTGTTAAAGGAGAGCAACTTCAGCGATGTCGTGAGCAAAAACCGGTATGTGCTGGTCGAGTTTTATGC
GCCCTGGTGCGGCCATTGCCAGCGCTTGGTTCCGGAGTATGCTGCGGCTGCGACTGAGTTGAAGGGGGAGGTCGTTCTGGCTAAAGTGGATGCCACTGAAGAAAATGATCTGGCGCAAAAATTCGAAGTTCAGGGGTTTCCGACCATCTTGTTTTTCATCGATGGAGTTCACAAGCAGTACCCCGGCCAAAGAACAAAAG
AAGCAATTGTTAATTGGATCAAGCGTAAAACTGGCCCTGCCGTATCCAACCTCACCACAACTGAGGATGCAGAGACTCTACTAGACTCAGGAACCACAGCTGCTGTTGGTTTGTTTGACTCATTAGAGGGCATTGAAAATGAGGAGTTTGAAGCAGCTTCCAGGCAAGAAGATGATGTGTTATTTTATCAAACAACTAGT
GAACGTGTAGCTGCTCTTCTCGACATTGACACAAAGGAAAAGCGGCCAGCATTGGTACTATTGAAGAAGGAGCCTGAAAAAATATCCCATTTTGATGGAAAATTTGAAAAAGCTGCCATATCTGAGTTCATCTTTGCAAACAAGCTCCCATTAGTGACGACCTTCACCAGGGAAAGTGCTAATCTGATATTTGACAGTTC
TATAAAGAAACAAATCCTGCTCTTTGTATCTGCCAAGGATTATGAAAAATTCATTCCATCATTTCAAGAAGCTGCAAAATCATTTAAGGGAAAGATCGTTTTTGTGCATGTTGATTCTGACAATGCTGATGTTGGGAAACCAATTATGGAATTCTTCGGCTTAAGTGGAGAAGAACCAAAGGTAATTGCATGCCTTTTAA
CTGAAGAACCCATCAAATATATGTTTGAAGCTGAAATAAATGCTGATAACATCAAGGCTTTTGGAGAGGACTTCCTTGCAGACAAACTCAAGCCTTTCTTCAAGTCCGACCCAGTGCCGGAAAAGAATGACGGAGATGTGAAAATTGTTGTGGGAAATAATTTTGATGAGATTGTACTGGATGAATCAAAGGATGTCCTT
CTCGAGTTATATGCACCTTGGTGTGGGCATTGCCAAGCATTGGAACCTATATACGATAAACTTGCAAAGCAGCTTCGTGGGGTTGACTCCCTTGTCATAGCTAAGATGGATGGTACAAGTAACGAGCATGCTAGGGCCAAGTCTGATGGGTATCCCACGATTTTATTCTATCCAGCAGGAAACAAGAGTTTTGATCCTAT
TACTTTTGATGGTGATCGCACTGTCAAAGGATTTTACAAATTCTTAAAGACAAATACAGCTATCCCATTCAAGTTGCCAAAGAAGAGCAAGACTGAAAGTGTAGAGGCTACTCCAGCTACTCAAGATACCAGTGCTGCTGACCAACCTAAAGATGAACTATGATGCTGAGTATTGATATTTGTGTACTTAGAATCGAAAT
TTTCCTCAATTTTCCCAAATTTTATCTCTATGAGGGCAATAAGCCAGTAATAAAGTTTTATAATTTATGAAAACCCTAAAAGCGCCAAGCAATTACACAATAACATTTCGGATTTAGTTGTAGTTTGATTTATCGCAAGATAAAATTGATTTCTTCAGATCCTGTTTGCCTACTTGTCACGTTTACATCCAAGATATTAA
AATTAATGGCCTGGTTCTATTAATAATTAACCATTTAAACACCTTCATCACTGAGGTGAGGTTTTTTCCAGGTATTAGAAGGGTTG

>TRINITY_DN110190_c0_g3_i3 from sample A2 / Example unspliced isoform:

GCTCGTTACAGTGTCACACACAGTCTTACAAACCCTCGGTCTCTCGAGTTCGTGCAGCCATGGCGGCCGCGAAGCTTGTGATCTTCGGTCTCGTGGCGCTTCTGTTGTCTGTCAGTTTCCAGCACCTTCAGGCCGTCGAAAATGACAGCGAAATCGACGAGATCGAAGAGTTGGAGCTCCTCGAAGACGAGGGGGAAGAA
AATACGACAATGGATCCTTTCACGGCCGGCGAGGAACCAGAGCTGGAAGAAGATTTTGAAGAAGGATCGTACGACCTGGAAGGTGAAGATGCCGATACCGATGCGCCGGCCGTCGACGAGAAAGATGTCGCGGTGTTAAAGGAGAGCAACTTCAGCGATGTCGTGAGCAAAAACCGGTATGTGCTGGTCGAGTTTTATGC
GCCCTGGTGCGGCCATTGCCAGCGCTTGGTTCCGGAGTATGCTGCGGCTGCGACTGAGTTGAAGGGGGAGGTCGTTCTGGCTAAAGTGGATGCCACTGAAGAAAATGATCTGGCGCAAAAATTCGAAGTTCAGGGGTTTCCGACCATCTTGTTTTTCATCGATGGAGTTCACAAGCAGTACCCCGGCCAAAGAACAAAAG
AAGCAATTGTTAATTGGATCAAGCGTAAAACTGGCCCTGCCGTATCCAACCTCACCACAACTGAGGATGCAGAGACTCTACTAGACTCAGGAACCACAGCTGCTGTTGGTTTGTTTGACTCATTAGAGGGCATTGAAAATGAGGAGTTTGAAGCAGCTTCCAGGCAAGAAGATGATGTGTTATTTTATCAAACAACTAGT
GAACGTGTAGCTGCTCTTCTCGACATTGACACAAAGGAAAAGCGGCCAGCATTGGTACTATTGAAGAAGGAGCCTGAAAAAATATCCCATTTTGATGGAAAATTTGAAAAAGCTGCCATATCTGAGTTCATCTTTGCAAACAAGCTCCCATTAGTGACGACCTTCACCAGGGAAAGTGCTAATCTGATATTTGACAGTTC
TATAAAGAAACAAATCCTGCTCTTTGTATCTGCCAAGGATTATGAAAAATTCATTCCATCATTTCAAGAAGCTGCAAAATCATTTAAGGGAAAGATCGTTTTTGTGCATGTTGATTCTGACAATGCTGATGTTGGGAAACCAATTATGGAATTCTTCGGCTTAAGTGGAGAAGAACCAAAGGTAATTGCATGCCTTTTAA
CTGAAGAACCCATCAAATATATGTTTGAAGCTGAAATAAATGCTGATAACATCAAGGCTTTTGGAGAGGACTTCCTTGCAGACAAACTCAAGCCTTTCTTCAAGTCCGACCCAGTGCCGGAAAAGgtttttgtttttaacttctcccttttacattgagttctttgttattcaggttttgatacaatgagttacctcttt
gccaattactaaattttccaggccataacgagtcacacctgtgttttgaattttgatagAATGACGGAGA