sp|B9GE13|KN12F_ORYSJ ----> Kinesin-like protein KIN-12F

Intron 1 GTACCTTGAT ... ATATTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.32	84	27	0
A2	31.82	75	35	6
A3	15.85	69	13	1
A4	23.74	35.3	11	4
A5	25	12	4	3
A6	19.72	57	14	1
A7	19.71	224	55	0
A8	21.26	263	71	1
A9	22.5	217	63	1
A10	19.44	145	35	1

>TRINITY_DN44769_c0_g1_i6 from sample A9 / Example spliced isoform

TTAATGATTTTGAATGCTCTGAAGAACACTGGAGAAGTGAAAGGGAAGGTCTCTCCTCTAAGATTGATCTTCTGCATGCTGCCCTTGTTGAAAAGGAAAAAGAGGTGGAAGTTCTTCAAAATCAACAAGTTACACAAGTTTCAGAAGCTGTTCCACGGTCTACTTTGAAGCAAGATATGGAAAATGAAGCTATCAAGTCA
GAACTGGAAAGAATGAGATTTGAATCTCAAAATCTCAAAGAAAATCTTTTTGAGAAAGATCAAACTATTTTATCGGTAAGAAAAGAAATGGAAGCTGCCCTAGCAAGCTTGAAGGAAGCTGAGCTGGAGATGAAGAGAACAATAGATGAAAAAGAAGAATTGAGGAAAGCTGAGCTGGAGATGAAGAGAACAATAGATGA
AAAAGAAGAATTGAGGAAAGCTGAGGAACAGGGAAGACATAAGATTGAATCCCTTATTAGTGACATCAAGTATTTGCAAGATCAGGTAAATCATAGAGAAGAAGAACTTGAGCTACTGCAGCTGGAGATGCCGAAATTGCTTGGAGCGGTGGAGAAGAGGATGAAGGATGCTGAAGAAGTCTGGCGAAAAGAGAAAGAGA
TATTATCTCTTGAGCTAAGTGATGCCAAGCTGATTGCTGCTGAAAAAAGCAGTGAGGCTGTCATTGTGTTTAGGAAGTTTGAGGAGAGCCAAGAGACTATTAAGGAAGCAGATCTCATGCTCAATACATTGTTGAAAGCAAATGAAAATGCAAGATATGACACAGAACAGTGGAAAAATGCAGGGCAAGATTTGGAAACT
GAAAGAGGTTCTTTGATAGATGAGATTCAAAGAACCAAAAATGCTTTTGAAGAGAAGGAAGAGCAATTTGTGTCAATGCAAGAGCAGATGCAGATGAATTTACAAGAAGCAAGAGACCTGATTTCCAGTGTAAAAGAAGTTGCTGTAAACTCAATGAGAGGACTTGAGGAAGAATTTGAGGCCATTAATACAGAAATCGG
TAACTTAAAGTTGGATATTTTACACGCCCTAAATGCACCAAATTCATGGCTTGAAGAGATCTTGTCTATTGTTGCTGAAAAAGAAAGTGCCACGTTTGTTTTGCATGCTTGCCAAGTAGAGGCACTCTTAGGAAAGCTGCGGGCACTGCATGCAGAGATTGATTCTCTTAATGTAAAACTACATGAATCCAACAGAAGTA
TATCTCAGCTGGAGGAGAATAACATGATCTTAAGGGGAGAAGTTGATAGGTCTTTCAGTTTGGGCACCAAATTAGAAGTGGAAATGAAGACAAGTTTAGGCAAAATTAATGAGAAAGAAAGACAACTGGAAAGGCTTTTGGCTCAATTAGACCAGTTGGATAAAAAAATGTCTGAATTAGAAGGACAGGAGGATGTGATG
ATGAAATGGTTGGCTGCTAAGGACTTTGATTTGGCTACCCTGATCTCACAGGTAGAAGAGAAGCACAATAATGAAATGGAAGTTGCTAGTAAACTTGAAATAATGCAAAGTGAAAAGGGGGAATTACATCTTCAACTCCAGAAGTCTTGTGAGAAGATTGAGCAGATGCAGAGAGAAAAAGAAGAATTATTTATTCAATT
AGAGCAGTCTTGTAACAAAATTACACTTATGGACAATCAACAAGAATCGATATGTAAAGAGATTGATTTGCTTCATGTGCAGCTTCATGAATCTAACACAAGGGTGGGTGAGTTAGAGAAGTATAACATGGATTTAAAAAATGAGATTAACGAGTATGAAGTGGCTATAGCCGAGTTGCAAGTGGATTTAATTGATGATG
ACTCAGAAAGAACTTTTGAAGCTGTCAAGGGACGAGAAAGCTTGGGTGGACAGCTGGAAACATTGAAAATGGAACTGTCTGATTTAAAGATGAAGAAGGAAACTCTAAGCAAAGCAATGATAGAGAAGGTATCCGAAGTTACAATTCTGCATCAAGAACTAGATCAACATACCAGGGTGCAGAGCTCTCTTCAGGAAACT
CTTCAACAGGTGGATGATTTGCAAAGAGAAAATCATGAGCTATCTCTTCAGCTGAAACAGTCTTCTGAAAGGATTATACATTTGGAAGAGGAAAAGACGGCTCTTGGACGGGAGGAATCTGAACTTCAAGAGCGATCAAGTTCATTACTCCTTGAACTTGATCAGAGTTGTGAAAGATCAGCTTATTTTGAGAAGCTTTC
TGCTGACGCTAATGAAAATTTGAAAAAGAAGCATGAAGAGATTGAAGCCCTGCATAGGCAGTTAGAGGAAGCATCTTGTAGTGCAATGTCCTTGGAACAGAAAAGTAAAGAATTACAAGACAAAGTAAGCACATTGGAGGCATCTTTTGGTGCTCTACGATCCAATCTTGATATTAAAAACAGAGATTTGATTGAGGTTC
AACTTGCTCATTCCAAAATTTTAGAGGAGATTGAACACAAGCACCAAGAGAGTGAGGACTTAATGACTAGATTGAATGCCTCTGAGATGGAAAACATGAAGTTGAGAGAGAATATTGCTAAAGAGCTTGAAACTAATGAAAGATTATTTTCTGAGCTTCAGCAAAGCAATGATGTTCTTGAAGAAATCCGGAGGGAAAAT
GCTAAACACGCTGTAGATATGCAAGAGAGAGAAGCAGAGATGATTAGGCTCTTTCAGCAGAAGGAAGACATTTCGAATCACTTTTATGAAGTGGAGAGAGAGAAGGAAACAATGAAAGAAACTATGAGCAGTCAAAATGACAAACTTCAAGAATTAGCTGAGCAGATAGCTCTTAAAGATGATTATTTGAAGCAGCTTCA
GGCTGTTTCTCTGCAATTGACAAAAGATAGCTGTTGTTCCACTGATATAGCTGAAAAGCTCGAGGCCAAACTTTCTAGCTTGCAGGACAGAGTTTTCTCTGTCATTCCTGTGGAGAGTGCAGATGAAATGCTGGTCATAGAAAAATTCCTTAAATACATATCTGAACTTGAGGAGAATGCACATATGTTTTTAGCTAAAA
CTGAATTGCTTGAGTTGAACTTCAAAGATATGTTTTCACAGAATGCTGCATTAATATCAGAACTGCAGTGGAAAGAGGAAATTTTGAAAGGTTTGCAATTTGATTTGAGTTTATTGCAAGAGTCTGCATCCAAAGCAAAGGACCAAAAAGATGAAGTTCAGGAGACATCTGCAGCATTGAAATTGGTTCAAGAAGATCTA
TCGGCAAAAAATAATGAGCTCGACACAGTTATGTCTCATAATATAAGGCTAGAGATAGAGCTAATGGAGAAAGTAAGCGAAGTGACCAGTCTAAACGTAGAGCTTACAAAACTAACGGAGACTCTGGCCTCTGTCTCAAATGAAAACTCTCAGCTTGCAACTGATATTGAAGACCTATTAGCAAAGAGAAATTCTGCAGA
AGAGGAAATGGAAGAGAAACAGAAAGTGATTGAAGGATTAGAGGAAGAGCTCCTTGAAATGAGTTCTCTGGTAGATCACCAGATGATCAATTCAATTGAAAGCATGAAATGTGAATTGAACAATGTTATTCGAGAGAGGGACCGTCTTAATGCAGAAGTACTTGCCCTGAATGAACAGTTGGAAATGACCCAGGCTTTTG
CAGATGAACGGGAAGCTATTGCTGTGGAAGCACGTCAGGTTGCAGAGGCAAGGAAAACTTATGCAGAAGAGAAAGAAGAAGAGACTAAGCTTTTGGAACGTTCAGTTGAAGAACTAGAGCGTACAGTAAATGCATTAGAGAGCCAGGTGGACATTGTGAAACGGGAGGCTGAAAGGCAGCGTCTGATGAGGGAGGATTTG
GAATTGGAGCTGCAAGCCCTAAGACATCAGATGTCAGCAATGCAGAGTGCACAAACCTCTATGAACATCCAAAGCTCAAGTGAGTCCATGGAGAGCGCCAATGAAAACCCAAGGGTTATGGCAGGTAGAAAGCTTGATTTGCGAGAGGCACGCAAACAGATTAAAGTTCTAGAGAAAGAACTTGAAGAAAAAGATGTGGA
GATTAAGAAGTGCAAAGCCCATATTACGGAATTGGTTATGAATGCAGAAACACAGGCAAGTGAGTACCAACAAAAGTATAAAGCATTGGAATCCATGGCAAATCAAGTGAAGGCTGAGCAAACGAACTCCAGTGCTATGGCTTCATATACGAATAAGACAGGGGAAAAGACTGCTGCTAAGTCTAGAGGTTCAGGGTCAC
CTTTCAAATGTATTGGTTTAGGTTTAGCCCAACAGATGAATTCAGAGAGGGATGAAGAGCTGCATGCTTCCAAGCGACGTATAGAAGAACTTGAAGGTCTTCTTGCAGCTCGACAAAAGGAGATATTCATGCTCAATTCTAGGCTGGCTGCAGCAGAGAGTATGACACATGATGTCATAAGGGATTTGCTTGGAGTTAAA
CTGGATATGACCAACTATGCTTCTGTACTAGATAATCAACAAGTACAAAAGATAGCAGAAAAAGCTCGGCGTCAGAATGAAGAGTCACAGGAAAAGGAACATGAAGTGCAGAAGCTAAGACATCAATTGAATGATTTAATTGAAGAAAGAGAGAGCTGGCTTGACGAAATTAATCGTAGACAAGCGGAAATGGTGGCTGC
TCGAATTGCAGCTGAAAAACTGCGACAGAGGGATCAGTTCCTGACTACAGAGAGTGATATGTTAAAGACAGACAATACTAGTCAGAAGAGGAAGATTGTGGAGCTTGAGGAAGAGATTAAGAAGCTTTCAGGGCAACAAAACCTACAACAACGAATTCATCATCATGCAAAAATTAAGGAAGAAAATAATACACTCAGAG
CTCAACATGAAGAAATCAGTGGAAGACTAAGGCGAACAGAGGCTCTTCTAGCTCGTGTAAATGATGAACTTGCTCGATACCGGACGGCTGATGGAAGGACACCTTATATTAGTATTGATGAAGAGCAAAGGCTGCGGAACAAACTTCAGGAAGCCGAGGAGGAAAGGGTGCAGTTGGCACAAAAGTTAGTGAGCTTGTGT
AACAGCATCTTGCAGGTAGCTGGAGTTGTTCGACCACCAAGGGACATTGATCCTTCCTTAGCAATTGAATCATTGCAGCAACTAAAAGAGAGACTTGATTGTGCAGAGAGAGAGTTGCAAGACTTGAAGCTCAAGACAAGAATCGCCAGTGAAAAGATGCGTCTGAGCGAGCTTCGCCAACAATCAAATTCTCCGTTAAG
TGTGAAGGCAAATGAGAACAATAACAGTTTTACAACTGTGCAGAACAACTGCCCAAGTCCTGCTAATTCTAATCCCATATAGAAAACGACTAAATGGTGTGGAGGCAGATGATAATTGAATGGATTAGAAGCCAACCATACTGATAGTTAAATTCTAGTTAACTAACATAACAAAGATGGATGTATTGGTTAAACCACTA
ATTTTCACATTGAGGGACAGTCGTTGTACAGCCCAGCATCAGTAGTCTTAAAACAATAACCGATTATTGTTTTTTGGGTTGAGGCATTCTTGCATTTATGCTCACCCTATGTAGATTATTCGTACTGTATCTCTCTATTTGTAACCATATTATATTGATTTCGTAATGCATTGCGATTATTGTTTGCAATTGAATAACGC
GGCTAATCTTAATTTTCAATTGTTCTCCAACCGAGTTAAGAAATTTGCAAGATATACAGTTGCAACCGAGTTAAGAAATTTGCAAAGATAATACAGTTGCAACCGAGTTAAGAAATTTGCAAAGATATACCGTTGCAAGACCATTTGAAGATTTACAAAAAATGAAGTTTTTTGAATACCCATTCACATTTTGACATATA
TATGACAAATTAATTTTC

>TRINITY_DN44769_c0_g1_i2 from sample A9 / Example unspliced isoform:

TTAATGATTTTGAATGCTCTGAAGAACACTGGAGAAGTGAAAGGGAAGGTCTCTCCTCTAAGATTGATCTTCTGCATGCTGCCCTTGTTGAAAAGGAAAAAGAGGTGGAAGTTCTTCAAAATCAACAAGTTACACAAGTTTCAGAAGCTGTTCCACGGTCTACTTTGAAGCAAGATATGGAAAATGAAGCTATCAAGTCA
GAACTGGAAAGAATGAGATTTGAATCTCAAAATCTCAAAGAAAATCTTTTTGAGAAAGATCAAACTATTTTATCGGTAAGAAAAGAAATGGAAGCTGCCCTAGCAAGCTTGAAGGAAGCTGAGCTGGAGATGAAGAGAACAATAGATGAAAAAGAAGAATTGAGGAAAGCTGAGCTGGAGATGAAGAGAACAATAGATGA
AAAAGAAGAATTGAGGAAAGCTGAGGAACAGGGAAGACATAAGATTGAATCCCTTATTAGTGACATCAAGTATTTGCAAGATCAGGTAAATCATAGAGAAGAAGAACTTGAGCTACTGCAGCTGGAGATGCCGAAATTGCTTGGAGCGGTGGAGAAGAGGATGAAGGATGCTGAAGAAGTCTGGCGAAAAGAGAAAGAGA
TATTATCTCTTGAGCTAAGTGATGCCAAGCTGATTGCTGCTGAAAAAAGCAGTGAGGCTGTCATTGTGTTTAGGAAGTTTGAGGAGAGCCAAGAGACTATTAAGGAAGCAGATCTCATGCTCAATACATTGTTGAAAGCAAATGAAAATGCAAGATATGACACAGAACAGTGGAAAAATGCAGGGCAAGATTTGGAAACT
GAAAGAGGTTCTTTGATAGATGAGATTCAAAGAACCAAAAATGCTTTTGAAGAGAAGGAAGAGCAATTTGTGTCAATGCAAGAGCAGATGCAGATGAATTTACAAGAAGCAAGAGACCTGATTTCCAGTGTAAAAGAAGTTGCTGTAAACTCAATGAGAGGACTTGAGGAAGAATTTGAGGCCATTAATACAGAAATCGG
TAACTTAAAGTTGGATATTTTACACGCCCTAAATGCACCAAATTCATGGCTTGAAGAGATCTTGTCTATTGTTGCTGAAAAAGAAAGTGCCACGTTTGTTTTGCATGCTTGCCAAGTAGAGGCACTCTTAGGAAAGCTGCGGGCACTGCATGCAGAGATTGATTCTCTTAATGTAAAACTACATGAATCCAACAGAAGTA
TATCTCAGCTGGAGGAGAATAACATGATCTTAAGGGGAGAAGTTGATAGGTCTTTCAGTTTGGGCACCAAATTAGAAGTGGAAATGAAGACAAGTTTAGGCAAAATTAATGAGAAAGAAAGACAACTGGAAAGGCTTTTGGCTCAATTAGACCAGTTGGATAAAAAAATGTCTGAATTAGAAGGACAGGAGGATGTGATG
ATGAAATGGTTGGCTGCTAAGGACTTTGATTTGGCTACCCTGATCTCACAGGTAGAAGAGAAGCACAATAATGAAATGGAAGTTGCTAGTAAACTTGAAATAATGCAAAGTGAAAAGGGGGAATTACATCTTCAACTCCAGAAGTCTTGTGAGAAGATTGAGCAGATGCAGAGAGAAAAAGAAGAATTATTTATTCAATT
AGAGCAGTCTTGTAACAAAATTACACTTATGGACAATCAACAAGAATCGATATGTAAAGAGATTGATTTGCTTCATGTGCAGCTTCATGAATCTAACACAAGGGTGGGTGAGTTAGAGAAGTATAACATGGATTTAAAAAATGAGATTAACGAGTATGAAGTGGCTATAGCCGAGTTGCAAGTGGATTTAATTGATGATG
ACTCAGAAAGAACTTTTGAAGCTGTCAAGGGACGAGAAAGCTTGGGTGGACAGCTGGAAACATTGAAAATGGAACTGTCTGATTTAAAGATGAAGAAGGAAACTCTAAGCAAAGCAATGATAGAGAAGGTATCCGAAGTTACAATTCTGCATCAAGAACTAGATCAACATACCAGGGTGCAGAGCTCTCTTCAGGAAACT
CTTCAACAGGTGGATGATTTGCAAAGAGAAAATCATGAGCTATCTCTTCAGCTGAAACAGTCTTCTGAAAGGATTATACATTTGGAAGAGGAAAAGACGGCTCTTGGACGGGAGGAATCTGAACTTCAAGAGCGATCAAGTTCATTACTCCTTGAACTTGATCAGAGTTGTGAAAGATCAGCTTATTTTGAGAAGCTTTC
TGCTGACGCTAATGAAAATTTGAAAAAGAAGCATGAAGAGATTGAAGCCCTGCATAGGCAGTTAGAGGAAGCATCTTGTAGTGCAATGTCCTTGGAACAGAAAAGTAAAGAATTACAAGACAAAGTAAGCACATTGGAGGCATCTTTTGGTGCTCTACGATCCAATCTTGATATTAAAAACAGAGATTTGATTGAGGTTC
AACTTGCTCATTCCAAAATTTTAGAGGAGATTGAACACAAGCACCAAGAGAGTGAGGACTTAATGACTAGATTGAATGCCTCTGAGATGGAAAACATGAAGTTGAGAGAGAATATTGCTAAAGAGCTTGAAACTAATGAAAGATTATTTTCTGAGCTTCAGCAAAGCAATGATGTTCTTGAAGAAATCCGGAGGGAAAAT
GCTAAACACGCTGTAGATATGCAAGAGAGAGAAGCAGAGATGATTAGGCTCTTTCAGCAGAAGGAAGACATTTCGAATCACTTTTATGAAGTGGAGAGAGAGAAGGAAACAATGAAAGAAACTATGAGCAGTCAAAATGACAAACTTCAAGAATTAGCTGAGCAGATAGCTCTTAAAGATGATTATTTGAAGCAGCTTCA
GGCTGTTTCTCTGCAATTGACAAAAGATAGCTGTTGTTCCACTGATATAGCTGAAAAGCTCGAGGCCAAACTTTCTAGCTTGCAGGACAGAGTTTTCTCTGTCATTCCTGTGGAGAGTGCAGATGAAATGCTGGTCATAGAAAAATTCCTTAAATACATATCTGAACTTGAGGAGAATGCACATATGTTTTTAGCTAAAA
CTGAATTGCTTGAGTTGAACTTCAAAGATATGTTTTCACAGAATGCTGCATTAATATCAGAACTGCAGTGGAAAGAGGAAATTTTGAAAGGTTTGCAATTTGATTTGAGTTTATTGCAAGAGTCTGCATCCAAAGCAAAGGACCAAAAAGATGAAGTTCAGGAGACATCTGCAGCATTGAAATTGGTTCAAGAAGATCTA
TCGGCAAAAAATAATGAGCTCGACACAGTTATGTCTCATAATATAAGGCTAGAGATAGAGCTAATGGAGAAAGTAAGCGAAGTGACCAGTCTAAACGTAGAGCTTACAAAACTAACGGAGACTCTGGCCTCTGTCTCAAATGAAAACTCTCAGCTTGCAACTGATATTGAAGACCTATTAGCAAAGAGAAATTCTGCAGA
AGAGGAAATGGAAGAGAAACAGAAAGTGATTGAAGGATTAGAGGAAGAGCTCCTTGAAATGAGTTCTCTGGTAGATCACCAGATGATCAATTCAATTGAAAGCATGAAATGTGAATTGAACAATGTTATTCGAGAGAGGGACCGTCTTAATGCAGAAGTACTTGCCCTGAATGAACAGTTGGAAATGACCCAGGCTTTTG
CAGATGAACGGGAAGCTATTGCTGTGGAAGCACGTCAGGTTGCAGAGGCAAGGAAAACTTATGCAGAAGAGAAAGAAGAAGAGACTAAGCTTTTGGAACGTTCAGTTGAAGAACTAGAGCGTACAGTAAATGCATTAGAGAGCCAGGTGGACATTGTGAAACGGGAGGCTGAAAGGCAGCGTCTGATGAGGGAGGATTTG
GAATTGGAGCTGCAAGCCCTAAGACATCAGATGTCAGCAATGCAGAGTGCACAAACCTCTATGAACATCCAAAGCTCAAGTGAGTCCATGGAGAGCGCCAATGAAAACCCAAGGGTTATGGCAGGTAGAAAGCTTGATTTGCGAGAGGCACGCAAACAGATTAAAGTTCTAGAGAAAGAACTTGAAGAAAAAGATGTGGA
GATTAAGAAGTGCAAAGCCCATATTACGGAATTGGTTATGAATGCAGAAACACAGGCAAGTGAGTACCAACAAAAGTATAAAGCATTGGAATCCATGGCAAATCAAGTGAAGGCTGAGCAAACGAACTCCAGTGCTATGGCTTCATATACGAATAAGACAGGGGAAAAGACTGCTGCTAAGTCTAGAGGTTCAGGGTCAC
CTTTCAAATGTATTGGTTTAGGTTTAGCCCAACAGATGAATTCAGAGAGGGATGAAGAGCTGCATGCTTCCAAGCGACGTATAGAAGAACTTGAAGGTCTTCTTGCAGCTCGACAAAAGGAGATATTCATGCTCAATTCTAGGCTGGCTGCAGCAGAGAGTATGACACATGATGTCATAAGGGATTTGCTTGGAGTTAAA
CTGGATATGACCAACTATGCTTCTGTACTAGATAATCAACAAGTACAAAAGATAGCAGAAAAAGCTCGGCGTCAGAATGAAGAGTCACAGGAAAAGGAACATGAAGTGCAGAAGCTAAGACATCAATTGAATGATTTAATTGAAGAAAGAGAGAGCTGGCTTGACGAAATTAATCGTAGACAAGCGGAAATGGTGGCTGC
TCGAATTGCAGCTGAAAAACTGCGACAGAGGGATCAGTTCCTGACTACAGAGAGTGATATGTTAAAGACAGACAATACTAGTCAGAAGAGGAAGATTGTGGAGCTTGAGGAAGAGATTAAGAAGCTTTCAGGGCAACAAAACCTACAACAACGAATTCATCATCATGCAAAAATTAAGGAAGAAAATAATACACTCAGAG
CTCAACATGAAGAAATCAGTGGAAGACTAAGGCGAACAGAGGCTCTTCTAGCTCGTGTAAATGATGAACTTGCTCGATACCGGACGGCTGATGGAAGGACACCTTATATTAGTATTGATGAAGAGCAAAGGCTGCGGAACAAACTTCAGgtaccttgatgccatcctatctatcctttgaccaaagaaagttgtagaccc
ttttaatctttcattttcgtgatgcgtctgcttttttgtcaaacaatggatttcccctaaagtaggaaaaagatgatgcttttcagaataaacactggcttgagtatttattaggcaatctgattccatatttccagGAAGCCGAGGAGGAAAGGGTGCAGTTGGCACAAAAGTTAGTGAGCTTGTGTAACAGCATCTTG
CAGGTTAGTAGAATGTACCGAATATTCATGGATTGTGTGCATGTACAGAATACTCTCTAAAACAATGCAAACATGGAACCAAACAGGTAGCTGGAGTTGTTCGACCACCAAGGGACATTGATCCTTCCTTAGCAATTGAATCATTGCAGCAACTAAAAGAGAGACTTGATTGTGCAGAGAGAGAGTTGCAAGACTTGAAG
CTCAAGACAAGAATCGCCAGTGAAAAGATGCGTCTGAGCGAGCTTCGCCAACAATCAAATTCTCCGTTAAGTGTGAAGGCAAATGAGAACAATAACAGTTTTACAACTGTGCAGAACAACTGCCCAAGTCCTGCTAATTCTAATCCCATATAGAAAACGACTAAATGGTGTGGAGGCAGATGATAATTGAATGGATTAGA
AGCCAACCATACTGATAGTTAAATTCTAGTTAACTAACATAACAAAGATGGATGTATTGGTTAAACCACTAATTTTCACATTGAGGGACAGTCGTTGTACAGCCCAGCATCAGTAGTCTTAAAACAATAACCGATTATTGTTTTTTGGGTTGAGGCATTCTTGCATTTATGCTCACCCTATGTAGATTATTCGTACTGTA
TCTCTCTATTTGTAACCATATTATATTGATTTCGTAATGCATTGCGATTATTGTTTGCAATTGAATAACGCGGCTAATCTTAATTTTCAATTGTTCTCCAACCGAGTTAAGAAATTTGCAA

Intron 2 GTTAGTAGAA ... AACCAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.31	125	2	38
A2	22.83	98	6	29
A3	24.22	97	2	31
A4	23.73	45	3	14
A5	25	18	4	6
A6	24.21	72	1	23
A7	17.84	350	1	76
A8	23.17	441	8	133
A9	19	324	3	76
A10	18.5	207	0	47

>TRINITY_DN44769_c0_g1_i6 from sample A9 / Example spliced isoform

TTAATGATTTTGAATGCTCTGAAGAACACTGGAGAAGTGAAAGGGAAGGTCTCTCCTCTAAGATTGATCTTCTGCATGCTGCCCTTGTTGAAAAGGAAAAAGAGGTGGAAGTTCTTCAAAATCAACAAGTTACACAAGTTTCAGAAGCTGTTCCACGGTCTACTTTGAAGCAAGATATGGAAAATGAAGCTATCAAGTCA
GAACTGGAAAGAATGAGATTTGAATCTCAAAATCTCAAAGAAAATCTTTTTGAGAAAGATCAAACTATTTTATCGGTAAGAAAAGAAATGGAAGCTGCCCTAGCAAGCTTGAAGGAAGCTGAGCTGGAGATGAAGAGAACAATAGATGAAAAAGAAGAATTGAGGAAAGCTGAGCTGGAGATGAAGAGAACAATAGATGA
AAAAGAAGAATTGAGGAAAGCTGAGGAACAGGGAAGACATAAGATTGAATCCCTTATTAGTGACATCAAGTATTTGCAAGATCAGGTAAATCATAGAGAAGAAGAACTTGAGCTACTGCAGCTGGAGATGCCGAAATTGCTTGGAGCGGTGGAGAAGAGGATGAAGGATGCTGAAGAAGTCTGGCGAAAAGAGAAAGAGA
TATTATCTCTTGAGCTAAGTGATGCCAAGCTGATTGCTGCTGAAAAAAGCAGTGAGGCTGTCATTGTGTTTAGGAAGTTTGAGGAGAGCCAAGAGACTATTAAGGAAGCAGATCTCATGCTCAATACATTGTTGAAAGCAAATGAAAATGCAAGATATGACACAGAACAGTGGAAAAATGCAGGGCAAGATTTGGAAACT
GAAAGAGGTTCTTTGATAGATGAGATTCAAAGAACCAAAAATGCTTTTGAAGAGAAGGAAGAGCAATTTGTGTCAATGCAAGAGCAGATGCAGATGAATTTACAAGAAGCAAGAGACCTGATTTCCAGTGTAAAAGAAGTTGCTGTAAACTCAATGAGAGGACTTGAGGAAGAATTTGAGGCCATTAATACAGAAATCGG
TAACTTAAAGTTGGATATTTTACACGCCCTAAATGCACCAAATTCATGGCTTGAAGAGATCTTGTCTATTGTTGCTGAAAAAGAAAGTGCCACGTTTGTTTTGCATGCTTGCCAAGTAGAGGCACTCTTAGGAAAGCTGCGGGCACTGCATGCAGAGATTGATTCTCTTAATGTAAAACTACATGAATCCAACAGAAGTA
TATCTCAGCTGGAGGAGAATAACATGATCTTAAGGGGAGAAGTTGATAGGTCTTTCAGTTTGGGCACCAAATTAGAAGTGGAAATGAAGACAAGTTTAGGCAAAATTAATGAGAAAGAAAGACAACTGGAAAGGCTTTTGGCTCAATTAGACCAGTTGGATAAAAAAATGTCTGAATTAGAAGGACAGGAGGATGTGATG
ATGAAATGGTTGGCTGCTAAGGACTTTGATTTGGCTACCCTGATCTCACAGGTAGAAGAGAAGCACAATAATGAAATGGAAGTTGCTAGTAAACTTGAAATAATGCAAAGTGAAAAGGGGGAATTACATCTTCAACTCCAGAAGTCTTGTGAGAAGATTGAGCAGATGCAGAGAGAAAAAGAAGAATTATTTATTCAATT
AGAGCAGTCTTGTAACAAAATTACACTTATGGACAATCAACAAGAATCGATATGTAAAGAGATTGATTTGCTTCATGTGCAGCTTCATGAATCTAACACAAGGGTGGGTGAGTTAGAGAAGTATAACATGGATTTAAAAAATGAGATTAACGAGTATGAAGTGGCTATAGCCGAGTTGCAAGTGGATTTAATTGATGATG
ACTCAGAAAGAACTTTTGAAGCTGTCAAGGGACGAGAAAGCTTGGGTGGACAGCTGGAAACATTGAAAATGGAACTGTCTGATTTAAAGATGAAGAAGGAAACTCTAAGCAAAGCAATGATAGAGAAGGTATCCGAAGTTACAATTCTGCATCAAGAACTAGATCAACATACCAGGGTGCAGAGCTCTCTTCAGGAAACT
CTTCAACAGGTGGATGATTTGCAAAGAGAAAATCATGAGCTATCTCTTCAGCTGAAACAGTCTTCTGAAAGGATTATACATTTGGAAGAGGAAAAGACGGCTCTTGGACGGGAGGAATCTGAACTTCAAGAGCGATCAAGTTCATTACTCCTTGAACTTGATCAGAGTTGTGAAAGATCAGCTTATTTTGAGAAGCTTTC
TGCTGACGCTAATGAAAATTTGAAAAAGAAGCATGAAGAGATTGAAGCCCTGCATAGGCAGTTAGAGGAAGCATCTTGTAGTGCAATGTCCTTGGAACAGAAAAGTAAAGAATTACAAGACAAAGTAAGCACATTGGAGGCATCTTTTGGTGCTCTACGATCCAATCTTGATATTAAAAACAGAGATTTGATTGAGGTTC
AACTTGCTCATTCCAAAATTTTAGAGGAGATTGAACACAAGCACCAAGAGAGTGAGGACTTAATGACTAGATTGAATGCCTCTGAGATGGAAAACATGAAGTTGAGAGAGAATATTGCTAAAGAGCTTGAAACTAATGAAAGATTATTTTCTGAGCTTCAGCAAAGCAATGATGTTCTTGAAGAAATCCGGAGGGAAAAT
GCTAAACACGCTGTAGATATGCAAGAGAGAGAAGCAGAGATGATTAGGCTCTTTCAGCAGAAGGAAGACATTTCGAATCACTTTTATGAAGTGGAGAGAGAGAAGGAAACAATGAAAGAAACTATGAGCAGTCAAAATGACAAACTTCAAGAATTAGCTGAGCAGATAGCTCTTAAAGATGATTATTTGAAGCAGCTTCA
GGCTGTTTCTCTGCAATTGACAAAAGATAGCTGTTGTTCCACTGATATAGCTGAAAAGCTCGAGGCCAAACTTTCTAGCTTGCAGGACAGAGTTTTCTCTGTCATTCCTGTGGAGAGTGCAGATGAAATGCTGGTCATAGAAAAATTCCTTAAATACATATCTGAACTTGAGGAGAATGCACATATGTTTTTAGCTAAAA
CTGAATTGCTTGAGTTGAACTTCAAAGATATGTTTTCACAGAATGCTGCATTAATATCAGAACTGCAGTGGAAAGAGGAAATTTTGAAAGGTTTGCAATTTGATTTGAGTTTATTGCAAGAGTCTGCATCCAAAGCAAAGGACCAAAAAGATGAAGTTCAGGAGACATCTGCAGCATTGAAATTGGTTCAAGAAGATCTA
TCGGCAAAAAATAATGAGCTCGACACAGTTATGTCTCATAATATAAGGCTAGAGATAGAGCTAATGGAGAAAGTAAGCGAAGTGACCAGTCTAAACGTAGAGCTTACAAAACTAACGGAGACTCTGGCCTCTGTCTCAAATGAAAACTCTCAGCTTGCAACTGATATTGAAGACCTATTAGCAAAGAGAAATTCTGCAGA
AGAGGAAATGGAAGAGAAACAGAAAGTGATTGAAGGATTAGAGGAAGAGCTCCTTGAAATGAGTTCTCTGGTAGATCACCAGATGATCAATTCAATTGAAAGCATGAAATGTGAATTGAACAATGTTATTCGAGAGAGGGACCGTCTTAATGCAGAAGTACTTGCCCTGAATGAACAGTTGGAAATGACCCAGGCTTTTG
CAGATGAACGGGAAGCTATTGCTGTGGAAGCACGTCAGGTTGCAGAGGCAAGGAAAACTTATGCAGAAGAGAAAGAAGAAGAGACTAAGCTTTTGGAACGTTCAGTTGAAGAACTAGAGCGTACAGTAAATGCATTAGAGAGCCAGGTGGACATTGTGAAACGGGAGGCTGAAAGGCAGCGTCTGATGAGGGAGGATTTG
GAATTGGAGCTGCAAGCCCTAAGACATCAGATGTCAGCAATGCAGAGTGCACAAACCTCTATGAACATCCAAAGCTCAAGTGAGTCCATGGAGAGCGCCAATGAAAACCCAAGGGTTATGGCAGGTAGAAAGCTTGATTTGCGAGAGGCACGCAAACAGATTAAAGTTCTAGAGAAAGAACTTGAAGAAAAAGATGTGGA
GATTAAGAAGTGCAAAGCCCATATTACGGAATTGGTTATGAATGCAGAAACACAGGCAAGTGAGTACCAACAAAAGTATAAAGCATTGGAATCCATGGCAAATCAAGTGAAGGCTGAGCAAACGAACTCCAGTGCTATGGCTTCATATACGAATAAGACAGGGGAAAAGACTGCTGCTAAGTCTAGAGGTTCAGGGTCAC
CTTTCAAATGTATTGGTTTAGGTTTAGCCCAACAGATGAATTCAGAGAGGGATGAAGAGCTGCATGCTTCCAAGCGACGTATAGAAGAACTTGAAGGTCTTCTTGCAGCTCGACAAAAGGAGATATTCATGCTCAATTCTAGGCTGGCTGCAGCAGAGAGTATGACACATGATGTCATAAGGGATTTGCTTGGAGTTAAA
CTGGATATGACCAACTATGCTTCTGTACTAGATAATCAACAAGTACAAAAGATAGCAGAAAAAGCTCGGCGTCAGAATGAAGAGTCACAGGAAAAGGAACATGAAGTGCAGAAGCTAAGACATCAATTGAATGATTTAATTGAAGAAAGAGAGAGCTGGCTTGACGAAATTAATCGTAGACAAGCGGAAATGGTGGCTGC
TCGAATTGCAGCTGAAAAACTGCGACAGAGGGATCAGTTCCTGACTACAGAGAGTGATATGTTAAAGACAGACAATACTAGTCAGAAGAGGAAGATTGTGGAGCTTGAGGAAGAGATTAAGAAGCTTTCAGGGCAACAAAACCTACAACAACGAATTCATCATCATGCAAAAATTAAGGAAGAAAATAATACACTCAGAG
CTCAACATGAAGAAATCAGTGGAAGACTAAGGCGAACAGAGGCTCTTCTAGCTCGTGTAAATGATGAACTTGCTCGATACCGGACGGCTGATGGAAGGACACCTTATATTAGTATTGATGAAGAGCAAAGGCTGCGGAACAAACTTCAGGAAGCCGAGGAGGAAAGGGTGCAGTTGGCACAAAAGTTAGTGAGCTTGTGT
AACAGCATCTTGCAGGTAGCTGGAGTTGTTCGACCACCAAGGGACATTGATCCTTCCTTAGCAATTGAATCATTGCAGCAACTAAAAGAGAGACTTGATTGTGCAGAGAGAGAGTTGCAAGACTTGAAGCTCAAGACAAGAATCGCCAGTGAAAAGATGCGTCTGAGCGAGCTTCGCCAACAATCAAATTCTCCGTTAAG
TGTGAAGGCAAATGAGAACAATAACAGTTTTACAACTGTGCAGAACAACTGCCCAAGTCCTGCTAATTCTAATCCCATATAGAAAACGACTAAATGGTGTGGAGGCAGATGATAATTGAATGGATTAGAAGCCAACCATACTGATAGTTAAATTCTAGTTAACTAACATAACAAAGATGGATGTATTGGTTAAACCACTA
ATTTTCACATTGAGGGACAGTCGTTGTACAGCCCAGCATCAGTAGTCTTAAAACAATAACCGATTATTGTTTTTTGGGTTGAGGCATTCTTGCATTTATGCTCACCCTATGTAGATTATTCGTACTGTATCTCTCTATTTGTAACCATATTATATTGATTTCGTAATGCATTGCGATTATTGTTTGCAATTGAATAACGC
GGCTAATCTTAATTTTCAATTGTTCTCCAACCGAGTTAAGAAATTTGCAAGATATACAGTTGCAACCGAGTTAAGAAATTTGCAAAGATAATACAGTTGCAACCGAGTTAAGAAATTTGCAAAGATATACCGTTGCAAGACCATTTGAAGATTTACAAAAAATGAAGTTTTTTGAATACCCATTCACATTTTGACATATA
TATGACAAATTAATTTTC

>TRINITY_DN44769_c0_g1_i2 from sample A9 / Example unspliced isoform:

TTAATGATTTTGAATGCTCTGAAGAACACTGGAGAAGTGAAAGGGAAGGTCTCTCCTCTAAGATTGATCTTCTGCATGCTGCCCTTGTTGAAAAGGAAAAAGAGGTGGAAGTTCTTCAAAATCAACAAGTTACACAAGTTTCAGAAGCTGTTCCACGGTCTACTTTGAAGCAAGATATGGAAAATGAAGCTATCAAGTCA
GAACTGGAAAGAATGAGATTTGAATCTCAAAATCTCAAAGAAAATCTTTTTGAGAAAGATCAAACTATTTTATCGGTAAGAAAAGAAATGGAAGCTGCCCTAGCAAGCTTGAAGGAAGCTGAGCTGGAGATGAAGAGAACAATAGATGAAAAAGAAGAATTGAGGAAAGCTGAGCTGGAGATGAAGAGAACAATAGATGA
AAAAGAAGAATTGAGGAAAGCTGAGGAACAGGGAAGACATAAGATTGAATCCCTTATTAGTGACATCAAGTATTTGCAAGATCAGGTAAATCATAGAGAAGAAGAACTTGAGCTACTGCAGCTGGAGATGCCGAAATTGCTTGGAGCGGTGGAGAAGAGGATGAAGGATGCTGAAGAAGTCTGGCGAAAAGAGAAAGAGA
TATTATCTCTTGAGCTAAGTGATGCCAAGCTGATTGCTGCTGAAAAAAGCAGTGAGGCTGTCATTGTGTTTAGGAAGTTTGAGGAGAGCCAAGAGACTATTAAGGAAGCAGATCTCATGCTCAATACATTGTTGAAAGCAAATGAAAATGCAAGATATGACACAGAACAGTGGAAAAATGCAGGGCAAGATTTGGAAACT
GAAAGAGGTTCTTTGATAGATGAGATTCAAAGAACCAAAAATGCTTTTGAAGAGAAGGAAGAGCAATTTGTGTCAATGCAAGAGCAGATGCAGATGAATTTACAAGAAGCAAGAGACCTGATTTCCAGTGTAAAAGAAGTTGCTGTAAACTCAATGAGAGGACTTGAGGAAGAATTTGAGGCCATTAATACAGAAATCGG
TAACTTAAAGTTGGATATTTTACACGCCCTAAATGCACCAAATTCATGGCTTGAAGAGATCTTGTCTATTGTTGCTGAAAAAGAAAGTGCCACGTTTGTTTTGCATGCTTGCCAAGTAGAGGCACTCTTAGGAAAGCTGCGGGCACTGCATGCAGAGATTGATTCTCTTAATGTAAAACTACATGAATCCAACAGAAGTA
TATCTCAGCTGGAGGAGAATAACATGATCTTAAGGGGAGAAGTTGATAGGTCTTTCAGTTTGGGCACCAAATTAGAAGTGGAAATGAAGACAAGTTTAGGCAAAATTAATGAGAAAGAAAGACAACTGGAAAGGCTTTTGGCTCAATTAGACCAGTTGGATAAAAAAATGTCTGAATTAGAAGGACAGGAGGATGTGATG
ATGAAATGGTTGGCTGCTAAGGACTTTGATTTGGCTACCCTGATCTCACAGGTAGAAGAGAAGCACAATAATGAAATGGAAGTTGCTAGTAAACTTGAAATAATGCAAAGTGAAAAGGGGGAATTACATCTTCAACTCCAGAAGTCTTGTGAGAAGATTGAGCAGATGCAGAGAGAAAAAGAAGAATTATTTATTCAATT
AGAGCAGTCTTGTAACAAAATTACACTTATGGACAATCAACAAGAATCGATATGTAAAGAGATTGATTTGCTTCATGTGCAGCTTCATGAATCTAACACAAGGGTGGGTGAGTTAGAGAAGTATAACATGGATTTAAAAAATGAGATTAACGAGTATGAAGTGGCTATAGCCGAGTTGCAAGTGGATTTAATTGATGATG
ACTCAGAAAGAACTTTTGAAGCTGTCAAGGGACGAGAAAGCTTGGGTGGACAGCTGGAAACATTGAAAATGGAACTGTCTGATTTAAAGATGAAGAAGGAAACTCTAAGCAAAGCAATGATAGAGAAGGTATCCGAAGTTACAATTCTGCATCAAGAACTAGATCAACATACCAGGGTGCAGAGCTCTCTTCAGGAAACT
CTTCAACAGGTGGATGATTTGCAAAGAGAAAATCATGAGCTATCTCTTCAGCTGAAACAGTCTTCTGAAAGGATTATACATTTGGAAGAGGAAAAGACGGCTCTTGGACGGGAGGAATCTGAACTTCAAGAGCGATCAAGTTCATTACTCCTTGAACTTGATCAGAGTTGTGAAAGATCAGCTTATTTTGAGAAGCTTTC
TGCTGACGCTAATGAAAATTTGAAAAAGAAGCATGAAGAGATTGAAGCCCTGCATAGGCAGTTAGAGGAAGCATCTTGTAGTGCAATGTCCTTGGAACAGAAAAGTAAAGAATTACAAGACAAAGTAAGCACATTGGAGGCATCTTTTGGTGCTCTACGATCCAATCTTGATATTAAAAACAGAGATTTGATTGAGGTTC
AACTTGCTCATTCCAAAATTTTAGAGGAGATTGAACACAAGCACCAAGAGAGTGAGGACTTAATGACTAGATTGAATGCCTCTGAGATGGAAAACATGAAGTTGAGAGAGAATATTGCTAAAGAGCTTGAAACTAATGAAAGATTATTTTCTGAGCTTCAGCAAAGCAATGATGTTCTTGAAGAAATCCGGAGGGAAAAT
GCTAAACACGCTGTAGATATGCAAGAGAGAGAAGCAGAGATGATTAGGCTCTTTCAGCAGAAGGAAGACATTTCGAATCACTTTTATGAAGTGGAGAGAGAGAAGGAAACAATGAAAGAAACTATGAGCAGTCAAAATGACAAACTTCAAGAATTAGCTGAGCAGATAGCTCTTAAAGATGATTATTTGAAGCAGCTTCA
GGCTGTTTCTCTGCAATTGACAAAAGATAGCTGTTGTTCCACTGATATAGCTGAAAAGCTCGAGGCCAAACTTTCTAGCTTGCAGGACAGAGTTTTCTCTGTCATTCCTGTGGAGAGTGCAGATGAAATGCTGGTCATAGAAAAATTCCTTAAATACATATCTGAACTTGAGGAGAATGCACATATGTTTTTAGCTAAAA
CTGAATTGCTTGAGTTGAACTTCAAAGATATGTTTTCACAGAATGCTGCATTAATATCAGAACTGCAGTGGAAAGAGGAAATTTTGAAAGGTTTGCAATTTGATTTGAGTTTATTGCAAGAGTCTGCATCCAAAGCAAAGGACCAAAAAGATGAAGTTCAGGAGACATCTGCAGCATTGAAATTGGTTCAAGAAGATCTA
TCGGCAAAAAATAATGAGCTCGACACAGTTATGTCTCATAATATAAGGCTAGAGATAGAGCTAATGGAGAAAGTAAGCGAAGTGACCAGTCTAAACGTAGAGCTTACAAAACTAACGGAGACTCTGGCCTCTGTCTCAAATGAAAACTCTCAGCTTGCAACTGATATTGAAGACCTATTAGCAAAGAGAAATTCTGCAGA
AGAGGAAATGGAAGAGAAACAGAAAGTGATTGAAGGATTAGAGGAAGAGCTCCTTGAAATGAGTTCTCTGGTAGATCACCAGATGATCAATTCAATTGAAAGCATGAAATGTGAATTGAACAATGTTATTCGAGAGAGGGACCGTCTTAATGCAGAAGTACTTGCCCTGAATGAACAGTTGGAAATGACCCAGGCTTTTG
CAGATGAACGGGAAGCTATTGCTGTGGAAGCACGTCAGGTTGCAGAGGCAAGGAAAACTTATGCAGAAGAGAAAGAAGAAGAGACTAAGCTTTTGGAACGTTCAGTTGAAGAACTAGAGCGTACAGTAAATGCATTAGAGAGCCAGGTGGACATTGTGAAACGGGAGGCTGAAAGGCAGCGTCTGATGAGGGAGGATTTG
GAATTGGAGCTGCAAGCCCTAAGACATCAGATGTCAGCAATGCAGAGTGCACAAACCTCTATGAACATCCAAAGCTCAAGTGAGTCCATGGAGAGCGCCAATGAAAACCCAAGGGTTATGGCAGGTAGAAAGCTTGATTTGCGAGAGGCACGCAAACAGATTAAAGTTCTAGAGAAAGAACTTGAAGAAAAAGATGTGGA
GATTAAGAAGTGCAAAGCCCATATTACGGAATTGGTTATGAATGCAGAAACACAGGCAAGTGAGTACCAACAAAAGTATAAAGCATTGGAATCCATGGCAAATCAAGTGAAGGCTGAGCAAACGAACTCCAGTGCTATGGCTTCATATACGAATAAGACAGGGGAAAAGACTGCTGCTAAGTCTAGAGGTTCAGGGTCAC
CTTTCAAATGTATTGGTTTAGGTTTAGCCCAACAGATGAATTCAGAGAGGGATGAAGAGCTGCATGCTTCCAAGCGACGTATAGAAGAACTTGAAGGTCTTCTTGCAGCTCGACAAAAGGAGATATTCATGCTCAATTCTAGGCTGGCTGCAGCAGAGAGTATGACACATGATGTCATAAGGGATTTGCTTGGAGTTAAA
CTGGATATGACCAACTATGCTTCTGTACTAGATAATCAACAAGTACAAAAGATAGCAGAAAAAGCTCGGCGTCAGAATGAAGAGTCACAGGAAAAGGAACATGAAGTGCAGAAGCTAAGACATCAATTGAATGATTTAATTGAAGAAAGAGAGAGCTGGCTTGACGAAATTAATCGTAGACAAGCGGAAATGGTGGCTGC
TCGAATTGCAGCTGAAAAACTGCGACAGAGGGATCAGTTCCTGACTACAGAGAGTGATATGTTAAAGACAGACAATACTAGTCAGAAGAGGAAGATTGTGGAGCTTGAGGAAGAGATTAAGAAGCTTTCAGGGCAACAAAACCTACAACAACGAATTCATCATCATGCAAAAATTAAGGAAGAAAATAATACACTCAGAG
CTCAACATGAAGAAATCAGTGGAAGACTAAGGCGAACAGAGGCTCTTCTAGCTCGTGTAAATGATGAACTTGCTCGATACCGGACGGCTGATGGAAGGACACCTTATATTAGTATTGATGAAGAGCAAAGGCTGCGGAACAAACTTCAGGTACCTTGATGCCATCCTATCTATCCTTTGACCAAAGAAAGTTGTAGACCC
TTTTAATCTTTCATTTTCGTGATGCGTCTGCTTTTTTGTCAAACAATGGATTTCCCCTAAAGTAGGAAAAAGATGATGCTTTTCAGAATAAACACTGGCTTGAGTATTTATTAGGCAATCTGATTCCATATTTCCAGGAAGCCGAGGAGGAAAGGGTGCAGTTGGCACAAAAGTTAGTGAGCTTGTGTAACAGCATCTTG
CAGgttagtagaatgtaccgaatattcatggattgtgtgcatgtacagaatactctctaaaacaatgcaaacatggaaccaaacagGTAGCTGGAGTTGTTCGACCACCAAGGGACATTGATCCTTCCTTAGCAATTGAATCATTGCAGCAACTAAAAGAGAGACTTGATTGTGCAGAGAGAGAGTTGCAAGACTTGAAG
CTCAAGACAAGAATCGCCAGTGAAAAGATGCGTCTGAGCGAGCTTCGCCAACAATCAAATTCTCCGTTAAGTGTGAAGGCAAATGAGAACAATAACAGTTTTACAACTGTGCAGAACAACTGCCCAAGTCCTGCTAATTCTAATCCCATATAGAAAACGACTAAATGGTGTGGAGGCAGATGATAATTGAATGGATTAGA
AGCCAACCATACTGATAGTTAAATTCTAGTTAACTAACATAACAAAGATGGATGTATTGGTTAAACCACTAATTTTCACATTGAGGGACAGTCGTTGTACAGCCCAGCATCAGTAGTCTTAAAACAATAACCGATTATTGTTTTTTGGGTTGAGGCATTCTTGCATTTATGCTCACCCTATGTAGATTATTCGTACTGTA
TCTCTCTATTTGTAACCATATTATATTGATTTCGTAATGCATTGCGATTATTGTTTGCAATTGAATAACGCGGCTAATCTTAATTTTCAATTGTTCTCCAACCGAGTTAAGAAATTTGCAA

Intron 3 GTAAGGAGAA ... GTCTAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.42	65	0	21
A2	16.67	75	3	15
A3	25.22	86	0	29
A4	40	21	3	14
A5	30.77	9	1	4
A6	50	7	1	7
A7	17.72	65	1	14
A8	21.9	82	2	23
A9	13.45	103	5	16
A10	10.39	69	2	8

>TRINITY_DN44769_c0_g1_i9 from sample A9 / Example spliced isoform

TTAATGATTTTGAATGCTCTGAAGAACACTGGAGAAGTGAAAGGGAAGGTCTCTCCTCTAAGATTGATCTTCTGCATGCTGCCCTTGTTGAAAAGGAAAAAGAGGTGGAAGTTCTTCAAAATCAACAAGTTACACAAGTTTCAGAAGCTGTTCCACGGTCTACTTTGAAGCAAGATATGGAAAATGAAGCTATCAAGTCA
GAACTGGAAAGAATGAGATTTGAATCTCAAAATCTCAAAGAAAATCTTTTTGAGAAAGATCAAACTATTTTATCGGTAAGAAAAGAAATGGAAGCTGCCCTAGCAAGCTTGAAGGAAGCTGAGCTGGAGATGAAGAGAACAATAGATGAAAAAGAAGAATTGAGGAAAGCTGAGCTGGAGATGAAGAGAACAATAGATGA
AAAAGAAGAATTGAGGAAAGCTGAGGAACAGGGAAGACATAAGATTGAATCCCTTATTAGTGACATCAAGTATTTGCAAGATCAGGTAAATCATAGAGAAGAAGAACTTGAGCTACTGCAGCTGGAGATGCCGAAATTGCTTGGAGCGGTGGAGAAGAGGATGAAGGATGCTGAAGAAGTCTGGCGAAAAGAGAAAGAGA
TATTATCTCTTGAGCTAAGTGATGCCAAGCTGATTGCTGCTGAAAAAAGCAGTGAGGCTGTCATTGTGTTTAGGAAGTTTGAGGAGAGCCAAGAGACTATTAAGGAAGCAGATCTCATGCTCAATACATTGTTGAAAGCAAATGAAAATGCAAGATATGACACAGAACAGTGGAAAAATGCAGGGCAAGATTTGGAAACT
GAAAGAGGTTCTTTGATAGATGAGATTCAAAGAACCAAAAATGCTTTTGAAGAGAAGGAAGAGCAATTTGTGTCAATGCAAGAGCAGATGCAGATGAATTTACAAGAAGCAAGAGACCTGATTTCCAGTGTAAAAGAAGTTGCTGTAAACTCAATGAGAGGACTTGAGGAAGAATTTGAGGCCATTAATACAGAAATCGG
TAACTTAAAGTTGGATATTTTACACGCCCTAAATGCACCAAATTCATGGCTTGAAGAGATCTTGTCTATTGTTGCTGAAAAAGAAAGTGCCACGTTTGTTTTGCATGCTTGCCAAGTAGAGGCACTCTTAGGAAAGCTGCGGGCACTGCATGCAGAGATTGATTCTCTTAATGTAAAACTACATGAATCCAACAGAAGTA
TATCTCAGCTGGAGGAGAATAACATGATCTTAAGGGGAGAAGTTGATAGGTCTTTCAGTTTGGGCACCAAATTAGAAGTGGAAATGAAGACAAGTTTAGGCAAAATTAATGAGAAAGAAAGACAACTGGAAAGGCTTTTGGCTCAATTAGACCAGTTGGATAAAAAAATGTCTGAATTAGAAGGACAGGAGGATGTGATG
ATGAAATGGTTGGCTGCTAAGGACTTTGATTTGGCTACCCTGATCTCACAGGTAGAAGAGAAGCACAATAATGAAATGGAAGTTGCTAGTAAACTTGAAATAATGCAAAGTGAAAAGGGGGAATTACATCTTCAACTCCAGAAGTCTTGTGAGAAGATTGAGCAGATGCAGAGAGAAAAAGAAGAATTATTTATTCAATT
AGAGCAGTCTTGTAACAAAATTACACTTATGGACAATCAACAAGAATCGATATGTAAAGAGATTGATTTGCTTCATGTGCAGCTTCATGAATCTAACACAAGGGTGGGTGAGTTAGAGAAGTATAACATGGATTTAAAAAATGAGATTAACGAGTATGAAGTGGCTATAGCCGAGTTGCAAGTGGATTTAATTGATGATG
ACTCAGAAAGAACTTTTGAAGCTGTCAAGGGACGAGAAAGCTTGGGTGGACAGCTGGAAACATTGAAAATGGAACTGTCTGATTTAAAGATGAAGAAGGAAACTCTAAGCAAAGCAATGATAGAGAAGGTATCCGAAGTTACAATTCTGCATCAAGAACTAGATCAACATACCAGGGTGCAGAGCTCTCTTCAGGAAACT
CTTCAACAGGTGGATGATTTGCAAAGAGAAAATCATGAGCTATCTCTTCAGCTGAAACAGTCTTCTGAAAGGATTATACATTTGGAAGAGGAAAAGACGGCTCTTGGACGGGAGGAATCTGAACTTCAAGAGCGATCAAGTTCATTACTCCTTGAACTTGATCAGAGTTGTGAAAGATCAGCTTATTTTGAGAAGCTTTC
TGCTGACGCTAATGAAAATTTGAAAAAGAAGCATGAAGAGATTGAAGCCCTGCATAGGCAGTTAGAGGAAGCATCTTGTAGTGCAATGTCCTTGGAACAGAAAAGTAAAGAATTACAAGACAAAGTAAGCACATTGGAGGCATCTTTTGGTGCTCTACGATCCAATCTTGATATTAAAAACAGAGATTTGATTGAGGTTC
AACTTGCTCATTCCAAAATTTTAGAGGAGATTGAACACAAGCACCAAGAGAGTGAGGACTTAATGACTAGATTGAATGCCTCTGAGATGGAAAACATGAAGTTGAGAGAGAATATTGCTAAAGAGCTTGAAACTAATGAAAGATTATTTTCTGAGCTTCAGCAAAGCAATGATGTTCTTGAAGAAATCCGGAGGGAAAAT
GCTAAACACGCTGTAGATATGCAAGAGAGAGAAGCAGAGATGATTAGGCTCTTTCAGCAGAAGGAAGACATTTCGAATCACTTTTATGAAGTGGAGAGAGAGAAGGAAACAATGAAAGAAACTATGAGCAGTCAAAATGACAAACTTCAAGAATTAGCTGAGCAGATAGCTCTTAAAGATGATTATTTGAAGCAGCTTCA
GGCTGTTTCTCTGCAATTGACAAAAGATAGCTGTTGTTCCACTGATATAGCTGAAAAGCTCGAGGCCAAACTTTCTAGCTTGCAGGACAGAGTTTTCTCTGTCATTCCTGTGGAGAGTGCAGATGAAATGCTGGTCATAGAAAAATTCCTTAAATACATATCTGAACTTGAGGAGAATGCACATATGTTTTTAGCTAAAA
CTGAATTGCTTGAGTTGAACTTCAAAGATATGTTTTCACAGAATGCTGCATTAATATCAGAACTGCAGTGGAAAGAGGAAATTTTGAAAGGTTTGCAATTTGATTTGAGTTTATTGCAAGAGTCTGCATCCAAAGCAAAGGACCAAAAAGATGAAGTTCAGGAGACATCTGCAGCATTGAAATTGGTTCAAGAAGATCTA
TCGGCAAAAAATAATGAGCTCGACACAGTTATGTCTCATAATATAAGGCTAGAGATAGAGCTAATGGAGAAAGTAAGCGAAGTGACCAGTCTAAACGTAGAGCTTACAAAACTAACGGAGACTCTGGCCTCTGTCTCAAATGAAAACTCTCAGCTTGCAACTGATATTGAAGACCTATTAGCAAAGAGAAATTCTGCAGA
AGAGGAAATGGAAGAGAAACAGAAAGTGATTGAAGGATTAGAGGAAGAGCTCCTTGAAATGAGTTCTCTGGTAGATCACCAGATGATCAATTCAATTGAAAGCATGAAATGTGAATTGAACAATGTTATTCGAGAGAGGGACCGTCTTAATGCAGAAGTACTTGCCCTGAATGAACAGTTGGAAATGACCCAGGCTTTTG
CAGATGAACGGGAAGCTATTGCTGTGGAAGCACGTCAGGTTGCAGAGGCAAGGAAAACTTATGCAGAAGAGAAAGAAGAAGAGACTAAGCTTTTGGAACGTTCAGTTGAAGAACTAGAGCGTACAGTAAATGCATTAGAGAGCCAGGTGGACATTGTGAAACGGGAGGCTGAAAGGCAGCGTCTGATGAGGGAGGATTTG
GAATTGGAGCTGCAAGCCCTAAGACATCAGATGTCAGCAATGCAGAGTGCACAAACCTCTATGAACATCCAAAGCTCAAGTGAGTCCATGGAGAGCGCCAATGAAAACCCAAGGGTTATGGCAGGTAGAAAGCTTGATTTGCGAGAGGCACGCAAACAGATTAAAGTTCTAGAGAAAGAACTTGAAGAAAAAGATGTGGA
GATTAAGAAGTGCAAAGCCCATATTACGGAATTGGTTATGAATGCAGAAACACAGGCAAGTGAGTACCAACAAAAGTATAAAGCATTGGAATCCATGGCAAATCAAGTGAAGGCTGAGCAAACGAACTCCAGTGCTATGGCTTCATATACGAATAAGACAGGGGAAAAGACTGCTGCTAAGTCTAGAGGTTCAGGGTCAC
CTTTCAAATGTATTGGTTTAGGTTTAGCCCAACAGATGAATTCAGAGAGGGATGAAGAGCTGCATGCTTCCAAGCGACGTATAGAAGAACTTGAAGGTCTTCTTGCAGCTCGACAAAAGGAGATATTCATGCTCAATTCTAGGCTGGCTGCAGCAGAGAGTATGACACATGATGTCATAAGGGATTTGCTTGGAGTTAAA
CTGGATATGACCAACTATGCTTCTGTACTAGATAATCAACAAGTACAAAAGATAGCAGAAAAAGCTCGGCGTCAGAATGAAGAGTCACAGGAAAAGGAACATGAAGTGCAGAAGCTAAGACATCAATTGAATGATTTAATTGAAGAAAGAGAGAGCTGGCTTGACGAAATTAATCGTAGACAAGCGGAAATGGTGGCTGC
TCGAATTGCAGCTGAAAAACTGCGACAGAGGGATCAGTTCCTGACTACAGAGAGTGATATGTTAAAGACAGACAATACTAGTCAGAAGAGGAAGATTGTGGAGCTTGAGGAAGAGATTAAGAAGCTTTCAGGGCAACAAAACCTACAACAACGAATTCATCATCATGCAAAAATTAAGGAAGAAAATAATACACTCAGAG
CTCAACATGAAGAAATCAGTGGAAGACTAAGGCGAACAGAGGCTCTTCTAGCTCGTGTAAATGATGAACTTGCTCGATACCGGACGGCTGATGGAAGGACACCTTATATTAGTATTGATGAAGAGCAAAGGCTGCGGAACAAACTTCAGGTACCTTGATGCCATCCTATCTATCCTTTGACCAAAGAAAGTTGTAGACCC
TTTTAATCTTTCATTTTCGTGATGCGTCTGCTTTTTTGTCAAACAATGGATTTCCCCTAAAGTAGGAAAAAGATGATGCTTTTCAGAATAAACACTGGCTTGAGTATTTATTAGGCAATCTGATTCCATATTTCCAGGAAGCCGAGGAGGAAAGGGTGCAGTTGGCACAAAAGTTAGTGAGCTTGTGTAACAGCATCTTG
CAGGTTAGTAGAATGTACCGAATATTCATGGATTGTGTGCATGTACAGAATACTCTCTAAAACAATGCAAACATGGAACCAAACAGGTAGCTGGAGTTGTTCGACCACCAAGGGACATTGATCCTTCCTTAGCAATTGAATCATTGCAGCAACTAAAAGAGAGACTTGATTGTGCAGAGAGAGAGTTGCAAGACTTGAAG
CTCAAGGTATTGATTCGAGTGCCATTATTACTTATTTTAGCATAGAGCTATATATTTTGAAACTAAAAATGAAGATTCACCTGGTTGAAAGACCCAGCAAAATTCACTCACAACTTCTTGATTTTTTGCTTCAGAGCATATAGATTTCTTGCACCATTCACTCTCACTTGCAACATGACACTCTGGTGTTTGCACACACC
ATTTATGACCAGCATTTCTTTTTGGTTCCGCATGATCATGCTGCCGCTTGACG

>TRINITY_DN44769_c0_g1_i4 from sample A9 / Example unspliced isoform:

TTAATGATTTTGAATGCTCTGAAGAACACTGGAGAAGTGAAAGGGAAGGTCTCTCCTCTAAGATTGATCTTCTGCATGCTGCCCTTGTTGAAAAGGAAAAAGAGGTGGAAGTTCTTCAAAATCAACAAGTTACACAAGTTTCAGAAGCTGTTCCACGGTCTACTTTGAAGCAAGATATGGAAAATGAAGCTATCAAGTCA
GAACTGGAAAGAATGAGATTTGAATCTCAAAATCTCAAAGAAAATCTTTTTGAGAAAGATCAAACTATTTTATCGGTAAGAAAAGAAATGGAAGCTGCCCTAGCAAGCTTGAAGGAAGCTGAGCTGGAGATGAAGAGAACAATAGATGAAAAAGAAGAATTGAGGAAAGCTGAGCTGGAGATGAAGAGAACAATAGATGA
AAAAGAAGAATTGAGGAAAGCTGAGGAACAGGGAAGACATAAGATTGAATCCCTTATTAGTGACATCAAGTATTTGCAAGATCAGGTAAATCATAGAGAAGAAGAACTTGAGCTACTGCAGCTGGAGATGCCGAAATTGCTTGGAGCGGTGGAGAAGAGGATGAAGGATGCTGAAGAAGTCTGGCGAAAAGAGAAAGAGA
TATTATCTCTTGAGCTAAGTGATGCCAAGCTGATTGCTGCTGAAAAAAGCAGTGAGGCTGTCATTGTGTTTAGGAAGTTTGAGGAGAGCCAAGAGACTATTAAGGAAGCAGATCTCATGCTCAATACATTGTTGAAAGCAAATGAAAATGCAAGATATGACACAGAACAGTGGAAAAATGCAGGGCAAGATTTGGAAACT
GAAAGAGGTTCTTTGATAGATGAGATTCAAAGAACCAAAAATGCTTTTGAAGAGAAGGAAGAGCAATTTGTGTCAATGCAAGAGCAGATGCAGATGAATTTACAAGAAGCAAGAGACCTGATTTCCAGTGTAAAAGAAGTTGCTGTAAACTCAATGAGAGGACTTGAGGAAGAATTTGAGGCCATTAATACAGAAATCGG
TAACTTAAAGTTGGATATTTTACACGCCCTAAATGCACCAAATTCATGGCTTGAAGAGATCTTGTCTATTGTTGCTGAAAAAGAAAGTGCCACGTTTGTTTTGCATGCTTGCCAAGTAGAGGCACTCTTAGGAAAGCTGCGGGCACTGCATGCAGAGATTGATTCTCTTAATGTAAAACTACATGAATCCAACAGAAGTA
TATCTCAGCTGGAGGAGAATAACATGATCTTAAGGGGAGAAGTTGATAGGTCTTTCAGTTTGGGCACCAAATTAGAAGTGGAAATGAAGACAAGTTTAGGCAAAATTAATGAGAAAGAAAGACAACTGGAAAGGCTTTTGGCTCAATTAGACCAGTTGGATAAAAAAATGTCTGAATTAGAAGGACAGGAGGATGTGATG
ATGAAATGGTTGGCTGCTAAGGACTTTGATTTGGCTACCCTGATCTCACAGGTAGAAGAGAAGCACAATAATGAAATGGAAGTTGCTAGTAAACTTGAAATAATGCAAAGTGAAAAGGGGGAATTACATCTTCAACTCCAGAAGTCTTGTGAGAAGATTGAGCAGATGCAGAGAGAAAAAGAAGAATTATTTATTCAATT
AGAGCAGTCTTGTAACAAAATTACACTTATGGACAATCAACAAGAATCGATATGTAAAGAGATTGATTTGCTTCATGTGCAGCTTCATGAATCTAACACAAGGGTGGGTGAGTTAGAGAAGTATAACATGGATTTAAAAAATGAGATTAACGAGTATGAAGTGGCTATAGCCGAGTTGCAAGTGGATTTAATTGATGATG
ACTCAGAAAGAACTTTTGAAGCTGTCAAGGGACGAGAAAGCTTGGGTGGACAGCTGGAAACATTGAAAATGGAACTGTCTGATTTAAAGATGAAGAAGGAAACTCTAAGCAAAGCAATGATAGAGAAGGTATCCGAAGTTACAATTCTGCATCAAGAACTAGATCAACATACCAGGGTGCAGAGCTCTCTTCAGGAAACT
CTTCAACAGGTGGATGATTTGCAAAGAGAAAATCATGAGCTATCTCTTCAGCTGAAACAGTCTTCTGAAAGGATTATACATTTGGAAGAGGAAAAGACGGCTCTTGGACGGGAGGAATCTGAACTTCAAGAGCGATCAAGTTCATTACTCCTTGAACTTGATCAGAGTTGTGAAAGATCAGCTTATTTTGAGAAGCTTTC
TGCTGACGCTAATGAAAATTTGAAAAAGAAGCATGAAGAGATTGAAGCCCTGCATAGGCAGTTAGAGGAAGCATCTTGTAGTGCAATGTCCTTGGAACAGAAAAGTAAAGAATTACAAGACAAAGTAAGCACATTGGAGGCATCTTTTGGTGCTCTACGATCCAATCTTGATATTAAAAACAGAGATTTGATTGAGGTTC
AACTTGCTCATTCCAAAATTTTAGAGGAGATTGAACACAAGCACCAAGAGAGTGAGGACTTAATGACTAGATTGAATGCCTCTGAGATGGAAAACATGAAGTTGAGAGAGAATATTGCTAAAGAGCTTGAAACTAATGAAAGATTATTTTCTGAGCTTCAGCAAAGCAATGATGTTCTTGAAGAAATCCGGAGGGAAAAT
GCTAAACACGCTGTAGATATGCAAGAGAGAGAAGCAGAGATGATTAGGCTCTTTCAGCAGAAGGAAGACATTTCGAATCACTTTTATGAAGTGGAGAGAGAGAAGGAAACAATGAAAGAAACTATGAGCAGTCAAAATGACAAACTTCAAGAATTAGCTGAGCAGATAGCTCTTAAAGATGATTATTTGAAGCAGCTTCA
GGCTGTTTCTCTGCAATTGACAAAAGATAGCTGTTGTTCCACTGATATAGCTGAAAAGCTCGAGGCCAAACTTTCTAGCTTGCAGGACAGAGTTTTCTCTGTCATTCCTGTGGAGAGTGCAGATGAAATGCTGGTCATAGAAAAATTCCTTAAATACATATCTGAACTTGAGGAGAATGCACATATGTTTTTAGCTAAAA
CTGAATTGCTTGAGTTGAACTTCAAAGATATGTTTTCACAGAATGCTGCATTAATATCAGAACTGCAGTGGAAAGAGGAAATTTTGAAAGGTTTGCAATTTGATTTGAGTTTATTGCAAGAGTCTGCATCCAAAGCAAAGGACCAAAAAGATGAAGTTCAGGAGACATCTGCAGCATTGAAATTGGTTCAAGAAGATCTA
TCGGCAAAAAATAATGAGCTCGACACAGTTATGTCTCATAATATAAGGCTAGAGATAGAGCTAATGGAGAAAGTAAGCGAAGTGACCAGTCTAAACGTAGAGCTTACAAAACTAACGGAGACTCTGGCCTCTGTCTCAAATGAAAACTCTCAGCTTGCAACTGATATTGAAGACCTATTAGCAAAGAGAAATTCTGCAGA
AGAGGAAATGGAAGAGAAACAGAAAGTGATTGAAGGATTAGAGGAAGAGCTCCTTGAAATGAGTTCTCTGGTAGATCACCAGATGATCAATTCAATTGAAAGCATGAAATGTGAATTGAACAATGTTATTCGAGAGAGGGACCGTCTTAATGCAGAAGTACTTGCCCTGAATGAACAGTTGGAAATGACCCAGGCTTTTG
CAGATGAACGGGAAGCTATTGCTGTGGAAGCACGTCAGGTTGCAGAGGCAAGGAAAACTTATGCAGAAGAGAAAGAAGAAGAGACTAAGCTTTTGGAACGTTCAGTTGAAGAACTAGAGCGTACAGTAAATGCATTAGAGAGCCAGGTGGACATTGTGAAACGGGAGGCTGAAAGGCAGCGTCTGATGAGGGAGGATTTG
GAATTGGAGCTGCAAGCCCTAAGACATCAGATGTCAGCAATGCAGAGTGCACAAACCTCTATGAACATCCAAAGCTCAAGTGAGTCCATGGAGAGCGCCAATGAAAACCCAAGGGTTATGGCAGGTAGAAAGCTTGATTTGCGAGAGGCACGCAAACAGATTAAAGTTCTAGAGAAAGAAATTGAAGAACAAGATGTGGA
GGTACCTAACTATTGAAATGGGAACTGTCTTAGAAAACTTAATTCACTGATACAAGCCCTTACCTCAGTCTCCCTTATCATGCAGATTAAGAAGTGCAAAGCCCATATTACGGAATTGGTTATGAATGCAGAAACACAGGCAAGTGAGTACCAACAAAAGgtaaggagaacaattgtattgtttaaaattttaatgcatt
cttgcacagtcagcagttcttgattcctccatgatgaaagcataaatatgattggttgtggatctaacttgtttttcccaactgtctaaacagTATAAAGCATTGGAATCCATGGCAAATCAAGTGAAGGCTGAGCAAACGAACTCCAGTGCTATGGCTTCATATACGAATAAGACAGGGGAAAAGACTGCTGCTAAGTC
TAGAGGTTCAGGGTCACCTTTCAAATGTATTGGTTTAGGTTTAGCCCAACAGATGAATTCAGAGAGGGATGAAGAGCTGCATGCTTCCAAGCGACGTATAGAAGAACTTGAAGGTCTTCTTGCAGCTCGACAAAAGGAGGTACATGGCTGCTCTTTGCAATGATACCTTGGAATTGCAGAACAATATTTTGAAAATCTAG
TGGCAAGTTGTGCAGATGAGGCCAGGTGTAAGTCCTGACTAATAGTAATTAGTAATTACCAAACCAAAGGAAAGCTGTAAGCAGCTTTTCTGAAAAAACAACCAAAAGTCAACATATTTATAAAATGAAATCAGTGATGGAATCCTCCAACTTTAGCTCATATTACCTTCTGCCATACCACTGATTTCGTTCAAGGATGT
ACTGATGATTTAAGTTTCTTGCATATCTATTTGATGGATGGCAGTGCTGATTTAACTAGCAATTCTTTTACTTGCAGGAGTAATTTTTTTAACCTGTATTGTATTTTGTATCATTATTATCTTTCTGTTCCTTCTGAGATATTTATGATGAATCCTAATCTTTTGATAGCAGAGAGCAGAACTTTGACCTATATTGTTTC
TGAAGCAGTTATTGTGTTTCTGCTTCTCAGATATTCATGCTCAATTCTAGG

Intron 4 GCAAACATAT ... CATGTCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.23	83	24	13
A2	16.8	52	4	17
A3	20	50	15	10
A4	26.32	28	10	10
A5	27.27	20	10	5
A6	24	38	11	13
A7	19.44	143	34	35
A8	17.22	161	36	31
A9	21.15	164	43	45
A10	18.78	93	25	18

>TRINITY_DN44769_c0_g1_i6 from sample A9 / Example spliced isoform

TTAATGATTTTGAATGCTCTGAAGAACACTGGAGAAGTGAAAGGGAAGGTCTCTCCTCTAAGATTGATCTTCTGCATGCTGCCCTTGTTGAAAAGGAAAAAGAGGTGGAAGTTCTTCAAAATCAACAAGTTACACAAGTTTCAGAAGCTGTTCCACGGTCTACTTTGAAGCAAGATATGGAAAATGAAGCTATCAAGTCA
GAACTGGAAAGAATGAGATTTGAATCTCAAAATCTCAAAGAAAATCTTTTTGAGAAAGATCAAACTATTTTATCGGTAAGAAAAGAAATGGAAGCTGCCCTAGCAAGCTTGAAGGAAGCTGAGCTGGAGATGAAGAGAACAATAGATGAAAAAGAAGAATTGAGGAAAGCTGAGCTGGAGATGAAGAGAACAATAGATGA
AAAAGAAGAATTGAGGAAAGCTGAGGAACAGGGAAGACATAAGATTGAATCCCTTATTAGTGACATCAAGTATTTGCAAGATCAGGTAAATCATAGAGAAGAAGAACTTGAGCTACTGCAGCTGGAGATGCCGAAATTGCTTGGAGCGGTGGAGAAGAGGATGAAGGATGCTGAAGAAGTCTGGCGAAAAGAGAAAGAGA
TATTATCTCTTGAGCTAAGTGATGCCAAGCTGATTGCTGCTGAAAAAAGCAGTGAGGCTGTCATTGTGTTTAGGAAGTTTGAGGAGAGCCAAGAGACTATTAAGGAAGCAGATCTCATGCTCAATACATTGTTGAAAGCAAATGAAAATGCAAGATATGACACAGAACAGTGGAAAAATGCAGGGCAAGATTTGGAAACT
GAAAGAGGTTCTTTGATAGATGAGATTCAAAGAACCAAAAATGCTTTTGAAGAGAAGGAAGAGCAATTTGTGTCAATGCAAGAGCAGATGCAGATGAATTTACAAGAAGCAAGAGACCTGATTTCCAGTGTAAAAGAAGTTGCTGTAAACTCAATGAGAGGACTTGAGGAAGAATTTGAGGCCATTAATACAGAAATCGG
TAACTTAAAGTTGGATATTTTACACGCCCTAAATGCACCAAATTCATGGCTTGAAGAGATCTTGTCTATTGTTGCTGAAAAAGAAAGTGCCACGTTTGTTTTGCATGCTTGCCAAGTAGAGGCACTCTTAGGAAAGCTGCGGGCACTGCATGCAGAGATTGATTCTCTTAATGTAAAACTACATGAATCCAACAGAAGTA
TATCTCAGCTGGAGGAGAATAACATGATCTTAAGGGGAGAAGTTGATAGGTCTTTCAGTTTGGGCACCAAATTAGAAGTGGAAATGAAGACAAGTTTAGGCAAAATTAATGAGAAAGAAAGACAACTGGAAAGGCTTTTGGCTCAATTAGACCAGTTGGATAAAAAAATGTCTGAATTAGAAGGACAGGAGGATGTGATG
ATGAAATGGTTGGCTGCTAAGGACTTTGATTTGGCTACCCTGATCTCACAGGTAGAAGAGAAGCACAATAATGAAATGGAAGTTGCTAGTAAACTTGAAATAATGCAAAGTGAAAAGGGGGAATTACATCTTCAACTCCAGAAGTCTTGTGAGAAGATTGAGCAGATGCAGAGAGAAAAAGAAGAATTATTTATTCAATT
AGAGCAGTCTTGTAACAAAATTACACTTATGGACAATCAACAAGAATCGATATGTAAAGAGATTGATTTGCTTCATGTGCAGCTTCATGAATCTAACACAAGGGTGGGTGAGTTAGAGAAGTATAACATGGATTTAAAAAATGAGATTAACGAGTATGAAGTGGCTATAGCCGAGTTGCAAGTGGATTTAATTGATGATG
ACTCAGAAAGAACTTTTGAAGCTGTCAAGGGACGAGAAAGCTTGGGTGGACAGCTGGAAACATTGAAAATGGAACTGTCTGATTTAAAGATGAAGAAGGAAACTCTAAGCAAAGCAATGATAGAGAAGGTATCCGAAGTTACAATTCTGCATCAAGAACTAGATCAACATACCAGGGTGCAGAGCTCTCTTCAGGAAACT
CTTCAACAGGTGGATGATTTGCAAAGAGAAAATCATGAGCTATCTCTTCAGCTGAAACAGTCTTCTGAAAGGATTATACATTTGGAAGAGGAAAAGACGGCTCTTGGACGGGAGGAATCTGAACTTCAAGAGCGATCAAGTTCATTACTCCTTGAACTTGATCAGAGTTGTGAAAGATCAGCTTATTTTGAGAAGCTTTC
TGCTGACGCTAATGAAAATTTGAAAAAGAAGCATGAAGAGATTGAAGCCCTGCATAGGCAGTTAGAGGAAGCATCTTGTAGTGCAATGTCCTTGGAACAGAAAAGTAAAGAATTACAAGACAAAGTAAGCACATTGGAGGCATCTTTTGGTGCTCTACGATCCAATCTTGATATTAAAAACAGAGATTTGATTGAGGTTC
AACTTGCTCATTCCAAAATTTTAGAGGAGATTGAACACAAGCACCAAGAGAGTGAGGACTTAATGACTAGATTGAATGCCTCTGAGATGGAAAACATGAAGTTGAGAGAGAATATTGCTAAAGAGCTTGAAACTAATGAAAGATTATTTTCTGAGCTTCAGCAAAGCAATGATGTTCTTGAAGAAATCCGGAGGGAAAAT
GCTAAACACGCTGTAGATATGCAAGAGAGAGAAGCAGAGATGATTAGGCTCTTTCAGCAGAAGGAAGACATTTCGAATCACTTTTATGAAGTGGAGAGAGAGAAGGAAACAATGAAAGAAACTATGAGCAGTCAAAATGACAAACTTCAAGAATTAGCTGAGCAGATAGCTCTTAAAGATGATTATTTGAAGCAGCTTCA
GGCTGTTTCTCTGCAATTGACAAAAGATAGCTGTTGTTCCACTGATATAGCTGAAAAGCTCGAGGCCAAACTTTCTAGCTTGCAGGACAGAGTTTTCTCTGTCATTCCTGTGGAGAGTGCAGATGAAATGCTGGTCATAGAAAAATTCCTTAAATACATATCTGAACTTGAGGAGAATGCACATATGTTTTTAGCTAAAA
CTGAATTGCTTGAGTTGAACTTCAAAGATATGTTTTCACAGAATGCTGCATTAATATCAGAACTGCAGTGGAAAGAGGAAATTTTGAAAGGTTTGCAATTTGATTTGAGTTTATTGCAAGAGTCTGCATCCAAAGCAAAGGACCAAAAAGATGAAGTTCAGGAGACATCTGCAGCATTGAAATTGGTTCAAGAAGATCTA
TCGGCAAAAAATAATGAGCTCGACACAGTTATGTCTCATAATATAAGGCTAGAGATAGAGCTAATGGAGAAAGTAAGCGAAGTGACCAGTCTAAACGTAGAGCTTACAAAACTAACGGAGACTCTGGCCTCTGTCTCAAATGAAAACTCTCAGCTTGCAACTGATATTGAAGACCTATTAGCAAAGAGAAATTCTGCAGA
AGAGGAAATGGAAGAGAAACAGAAAGTGATTGAAGGATTAGAGGAAGAGCTCCTTGAAATGAGTTCTCTGGTAGATCACCAGATGATCAATTCAATTGAAAGCATGAAATGTGAATTGAACAATGTTATTCGAGAGAGGGACCGTCTTAATGCAGAAGTACTTGCCCTGAATGAACAGTTGGAAATGACCCAGGCTTTTG
CAGATGAACGGGAAGCTATTGCTGTGGAAGCACGTCAGGTTGCAGAGGCAAGGAAAACTTATGCAGAAGAGAAAGAAGAAGAGACTAAGCTTTTGGAACGTTCAGTTGAAGAACTAGAGCGTACAGTAAATGCATTAGAGAGCCAGGTGGACATTGTGAAACGGGAGGCTGAAAGGCAGCGTCTGATGAGGGAGGATTTG
GAATTGGAGCTGCAAGCCCTAAGACATCAGATGTCAGCAATGCAGAGTGCACAAACCTCTATGAACATCCAAAGCTCAAGTGAGTCCATGGAGAGCGCCAATGAAAACCCAAGGGTTATGGCAGGTAGAAAGCTTGATTTGCGAGAGGCACGCAAACAGATTAAAGTTCTAGAGAAAGAACTTGAAGAAAAAGATGTGGA
GATTAAGAAGTGCAAAGCCCATATTACGGAATTGGTTATGAATGCAGAAACACAGGCAAGTGAGTACCAACAAAAGTATAAAGCATTGGAATCCATGGCAAATCAAGTGAAGGCTGAGCAAACGAACTCCAGTGCTATGGCTTCATATACGAATAAGACAGGGGAAAAGACTGCTGCTAAGTCTAGAGGTTCAGGGTCAC
CTTTCAAATGTATTGGTTTAGGTTTAGCCCAACAGATGAATTCAGAGAGGGATGAAGAGCTGCATGCTTCCAAGCGACGTATAGAAGAACTTGAAGGTCTTCTTGCAGCTCGACAAAAGGAGATATTCATGCTCAATTCTAGGCTGGCTGCAGCAGAGAGTATGACACATGATGTCATAAGGGATTTGCTTGGAGTTAAA
CTGGATATGACCAACTATGCTTCTGTACTAGATAATCAACAAGTACAAAAGATAGCAGAAAAAGCTCGGCGTCAGAATGAAGAGTCACAGGAAAAGGAACATGAAGTGCAGAAGCTAAGACATCAATTGAATGATTTAATTGAAGAAAGAGAGAGCTGGCTTGACGAAATTAATCGTAGACAAGCGGAAATGGTGGCTGC
TCGAATTGCAGCTGAAAAACTGCGACAGAGGGATCAGTTCCTGACTACAGAGAGTGATATGTTAAAGACAGACAATACTAGTCAGAAGAGGAAGATTGTGGAGCTTGAGGAAGAGATTAAGAAGCTTTCAGGGCAACAAAACCTACAACAACGAATTCATCATCATGCAAAAATTAAGGAAGAAAATAATACACTCAGAG
CTCAACATGAAGAAATCAGTGGAAGACTAAGGCGAACAGAGGCTCTTCTAGCTCGTGTAAATGATGAACTTGCTCGATACCGGACGGCTGATGGAAGGACACCTTATATTAGTATTGATGAAGAGCAAAGGCTGCGGAACAAACTTCAGGAAGCCGAGGAGGAAAGGGTGCAGTTGGCACAAAAGTTAGTGAGCTTGTGT
AACAGCATCTTGCAGGTAGCTGGAGTTGTTCGACCACCAAGGGACATTGATCCTTCCTTAGCAATTGAATCATTGCAGCAACTAAAAGAGAGACTTGATTGTGCAGAGAGAGAGTTGCAAGACTTGAAGCTCAAGACAAGAATCGCCAGTGAAAAGATGCGTCTGAGCGAGCTTCGCCAACAATCAAATTCTCCGTTAAG
TGTGAAGGCAAATGAGAACAATAACAGTTTTACAACTGTGCAGAACAACTGCCCAAGTCCTGCTAATTCTAATCCCATATAGAAAACGACTAAATGGTGTGGAGGCAGATGATAATTGAATGGATTAGAAGCCAACCATACTGATAGTTAAATTCTAGTTAACTAACATAACAAAGATGGATGTATTGGTTAAACCACTA
ATTTTCACATTGAGGGACAGTCGTTGTACAGCCCAGCATCAGTAGTCTTAAAACAATAACCGATTATTGTTTTTTGGGTTGAGGCATTCTTGCATTTATGCTCACCCTATGTAGATTATTCGTACTGTATCTCTCTATTTGTAACCATATTATATTGATTTCGTAATGCATTGCGATTATTGTTTGCAATTGAATAACGC
GGCTAATCTTAATTTTCAATTGTTCTCCAACCGAGTTAAGAAATTTGCAAGATATACAGTTGCAACCGAGTTAAGAAATTTGCAAAGATAATACAGTTGCAACCGAGTTAAGAAATTTGCAAAGATATACCGTTGCAAGACCATTTGAAGATTTACAAAAAATGAAGTTTTTTGAATACCCATTCACATTTTGACATATA
TATGACAAATTAATTTTC

>TRINITY_DN44769_c0_g1_i5 from sample A9 / Example unspliced isoform:

TTAATGATTTTGAATGCTCTGAAGAACACTGGAGAAGTGAAAGGGAAGGTCTCTCCTCTAAGATTGATCTTCTGCATGCTGCCCTTGTTGAAAAGGAAAAAGAGGTGGAAGTTCTTCAAAATCAACAAGTTACACAAGTTTCAGAAGCTGTTCCACGGTCTACTTTGAAGCAAGATATGGAAAATGAAGCTATCAAGTCA
GAACTGGAAAGAATGAGATTTGAATCTCAAAATCTCAAAGAAAATCTTTTTGAGAAAGATCAAACTATTTTATCGGTAAGAAAAGAAATGGAAGCTGCCCTAGCAAGCTTGAAGGAAGCTGAGCTGGAGATGAAGAGAACAATAGATGAAAAAGAAGAATTGAGGAAAGCTGAGCTGGAGATGAAGAGAACAATAGATGA
AAAAGAAGAATTGAGGAAAGCTGAGGAACAGGGAAGACATAAGATTGAATCCCTTATTAGTGACATCAAGTATTTGCAAGATCAGGTAAATCATAGAGAAGAAGAACTTGAGCTACTGCAGCTGGAGATGCCGAAATTGCTTGGAGCGGTGGAGAAGAGGATGAAGGATGCTGAAGAAGTCTGGCGAAAAGAGAAAGAGA
TATTATCTCTTGAGCTAAGTGATGCCAAGCTGATTGCTGCTGAAAAAAGCAGTGAGGCTGTCATTGTGTTTAGGAAGTTTGAGGAGAGCCAAGAGACTATTAAGGAAGCAGATCTCATGCTCAATACATTGTTGAAAGCAAATGAAAATGCAAGATATGACACAGAACAGTGGAAAAATGCAGGGCAAGATTTGGAAACT
GAAAGAGGTTCTTTGATAGATGAGATTCAAAGAACCAAAAATGCTTTTGAAGAGAAGGAAGAGCAATTTGTGTCAATGCAAGAGCAGATGCAGATGAATTTACAAGAAGCAAGAGACCTGATTTCCAGTGTAAAAGAAGTTGCTGTAAACTCAATGAGAGGACTTGAGGAAGAATTTGAGGCCATTAATACAGAAATCGG
TAACTTAAAGTTGGATATTTTACACGCCCTAAATGCACCAAATTCATGGCTTGAAGAGATCTTGTCTATTGTTGCTGAAAAAGAAAGTGCCACGTTTGTTTTGCATGCTTGCCAAGTAGAGGCACTCTTAGGAAAGCTGCGGGCACTGCATGCAGAGATTGATTCTCTTAATGTAAAACTACATGAATCCAACAGAAGTA
TATCTCAGCTGGAGGAGAATAACATGATCTTAAGGGGAGAAGTTGATAGGTCTTTCAGTTTGGGCACCAAATTAGAAGTGGAAATGAAGACAAGTTTAGGCAAAATTAATGAGAAAGAAAGACAACTGGAAAGGCTTTTGGCTCAATTAGACCAGTTGGATAAAAAAATGTCTGAATTAGAAGGACAGGAGGATGTGATG
ATGAAATGGTTGGCTGCTAAGGACTTTGATTTGGCTACCCTGATCTCACAGGTAGAAGAGAAGCACAATAATGAAATGGAAGTTGCTAGTAAACTTGAAATAATGCAAAGTGAAAAGGGGGAATTACATCTTCAACTCCAGAAGTCTTGTGAGAAGATTGAGCAGATGCAGAGAGAAAAAGAAGAATTATTTATTCAATT
AGAGCAGTCTTGTAACAAAATTACACTTATGGACAATCAACAAGAATCGATATGTAAAGAGATTGATTTGCTTCATGTGCAGCTTCATGAATCTAACACAAGGGTGGGTGAGTTAGAGAAGTATAACATGGATTTAAAAAATGAGATTAACGAGTATGAAGTGGCTATAGCCGAGTTGCAAGTGGATTTAATTGATGATG
ACTCAGAAAGAACTTTTGAAGCTGTCAAGGGACGAGAAAGCTTGGGTGGACAGCTGGAAACATTGAAAATGGAACTGTCTGATTTAAAGATGAAGAAGGAAACTCTAAGCAAAGCAATGATAGAGAAGGTATCCGAAGTTACAATTCTGCATCAAGAACTAGATCAACATACCAGGGTGCAGAGCTCTCTTCAGGAAACT
CTTCAACAGGTGGATGATTTGCAAAGAGAAAATCATGAGCTATCTCTTCAGCTGAAACAGTCTTCTGAAAGGATTATACATTTGGAAGAGGAAAAGACGGCTCTTGGACGGGAGGAATCTGAACTTCAAGAGCGATCAAGTTCATTACTCCTTGAACTTGATCAGAGTTGTGAAAGATCAGCTTATTTTGAGAAGCTTTC
TGCTGACGCTAATGAAAATTTGAAAAAGAAGCATGAAGAGATTGAAGCCCTGCATAGGCAGTTAGAGGAAGCATCTTGTAGTGCAATGTCCTTGGAACAGAAAAGTAAAGAATTACAAGACAAAGTAAGCACATTGGAGGCATCTTTTGGTGCTCTACGATCCAATCTTGATATTAAAAACAGAGATTTGATTGAGGTTC
AACTTGCTCATTCCAAAATTTTAGAGGAGATTGAACACAAGCACCAAGAGAGTGAGGACTTAATGACTAGATTGAATGCCTCTGAGATGGAAAACATGAAGTTGAGAGAGAATATTGCTAAAGAGCTTGAAACTAATGAAAGATTATTTTCTGAGCTTCAGCAAAGCAATGATGTTCTTGAAGAAATCCGGAGGGAAAAT
GCTAAACACGCTGTAGATATGCAAGAGAGAGAAGCAGAGATGATTAGGCTCTTTCAGCAGAAGGAAGACATTTCGAATCACTTTTATGAAGTGGAGAGAGAGAAGGAAACAATGAAAGAAACTATGAGCAGTCAAAATGACAAACTTCAAGAATTAGCTGAGCAGATAGCTCTTAAAGATGATTATTTGAAGCAGCTTCA
GGCTGTTTCTCTGCAATTGACAAAAGATAGCTGTTGTTCCACTGATATAGCTGAAAAGCTCGAGGCCAAACTTTCTAGCTTGCAGGACAGAGTTTTCTCTGTCATTCCTGTGGAGAGTGCAGATGAAATGCTGGTCATAGAAAAATTCCTTAAATACATATCTGAACTTGAGGAGAATGCACATATGTTTTTAGCTAAAA
CTGAATTGCTTGAGTTGAACTTCAAAGATATGTTTTCACAGAATGCTGCATTAATATCAGAACTGCAGTGGAAAGAGGAAATTTTGAAAGGTTTGCAATTTGATTTGAGTTTATTGCAAGAGTCTGCATCCAAAGCAAAGGACCAAAAAGATGAAGTTCAGGAGACATCTGCAGCATTGAAATTGGTTCAAGAAGATCTA
TCGGCAAAAAATAATGAGCTCGACACAGTTATGTCTCATAATATAAGGCTAGAGATAGAGCTAATGGAGAAAGTAAGCGAAGTGACCAGTCTAAACGTAGAGCTTACAAAACTAACGGAGACTCTGGCCTCTGTCTCAAATGAAAACTCTCAGCTTGCAACTGATATTGAAGACCTATTAGCAAAGAGAAATTCTGCAGA
AGAGGAAATGGAAGAGAAACAGAAAGTGATTGAAGGATTAGAGGAAGAGCTCCTTGAAATGAGTTCTCTGGTAGATCACCAGATGATCAATTCAATTGAAAGCATGAAATGTGAATTGAACAATGTTATTCGAGAGAGGGACCGTCTTAATGCAGAAGTACTTGCCCTGAATGAACAGTTGGAAATGACCCAGGCTTTTG
CAGATGAACGGGAAGCTATTGCTGTGGAAGCACGTCAGGTTGCAGAGGCAAGGAAAACTTATGCAGAAGAGAAAGAAGAAGAGACTAAGCTTTTGGAACGTTCAGTTGAAGAACTAGAGCGTACAGTAAATGCATTAGAGAGCCAGGTGGACATTGTGAAACGGGAGGCTGAAAGGCAGCGTCTGATGAGGGAGGATTTG
GAATTGGAGCTGCAAGCCCTAAGACATCAGATGTCAGCAATGCAGAGTGCACAAACCTCTATGAACATCCAAAGCTCAAGTGAGTCCATGGAGAGCGCCAATGAAAACCCAAGGGTTATGGCAGGTAGAAAGCTTGATTTGCGAGAGGCACGCAAACAGATTAAAGTTCTAGAGAAAGAACTTGAAGAAAAAGATGTGGA
GATTAAGAAGTGCAAAGCCCATATTACGGAATTGGTTATGAATGCAGAAACACAGGCAAGTGAGTACCAACAAAAGTATAAAGCATTGGAATCCATGGCAAATCAAGTGAAGGCTGAGCAAACGAACTCCAGTGCTATGGCTTCATATACGAATAAGACAGGGGAAAAGACTGCTGCTAAGTCTAGAGGTTCAGGGTCAC
CTTTCAAATGTATTGGTTTAGGTTTAGCCCAACAGATGAATTCAGAGAGGGATGAAGAGCTGCATGCTTCCAAGCGACGTATAGAAGAACTTGAAGGTCTTCTTGCAGCTCGACAAAAGGAGATATTCATGCTCAATTCTAGGCTGGCTGCAGCAGAGAGTATGACACATGATGTCATAAGGGATTTGCTTGGAGTTAAA
CTGGATATGACCAACTATGCTTCTGTACTAGATAATCAACAAGTACAAAAGATAGCAGAAAAAGCTCGGCGTCAGAATGAAGAGTCACAGGAAAAGGAACATGAAGTGCAGAAGCTAAGACATCAATTGAATGATTTAATTGAAGAAAGAGAGAGCTGGCTTGACGAAATTAATCGTAGACAAGCGGAAATGGTGGCTGC
TCGAATTGCAGCTGAAAAACTGCGACAGAGGGATCAGTTCCTGACTACAGAGAGTGATATGTTAAAGACAGACAATACTAGTCAGAAGAGGAAGATTGTGGAGCTTGAGGAAGAGATTAAGAAGCTTTCAGGGCAACAAAACCTACAACAACGAATTCATCATCATGCAAAAATTAAGgcaaacatattttatctaaaca
tattagtttagtttgtaataagaggatattgcaagatgatataagggtattttcatttggagcaaatagattcgtgtttataatataagtggacattagtaggacattttatactttttcataaatttattctgaatgttaaaattcaatgctgcttcatgtcgcagGAAGAAAATAATACACTCAGAGCTCAACATGAA
GAAATCAGTGGAAGACTAAGGCGAACAGAGGCTCTTCTAGCTCGTGTAAATGATGAACTTGCTCGATACCGGACGGCTGATGGAAGGACACCTTATATTAGTATTGATGAAGAGCAAAGGCTGCGGAACAAACTTCAGGAAGCCGAGGAGGAAAGGGTGCAGTTGGCACAAAAGTTAGTGAGCTTGTGTAACAGCATCTT
GCAGGTAGCTGGAGTTGTTCGACCACCAAGGGACATTGATCCTTCCTTAGCAATTGAATCATTGCAGCAACTAAAAGAGAGACTTGATTGTGCAGAGAGAGAGTTGCAAGACTTGAAGCTCAAGACAAGAATCGCCAGTGAAAAGATGCGTCTGAGCGAGCTTCGCCAACAATCAAATTCTCCGTTAAGTGTGAAGGCAA
ATGAGAACAATAACAGTTTTACAACTGTGCAGAACAACTGCCCAAGTCCTGCTAATTCTAATCCCATATAGAAAACGACTAAATGGTGTGGAGGCAGATGATAATTGAATGGATTAGAAGCCAACCATACTGATAGTTAAATTCTAGTTAACTAACATAACAAAGATGGATGTATTGGTTAAACCACTAATTTTCACATT
GAGGGACAGTCGTTGTACAGCCCAGCATCAGTAGTCTTAAAACAATAACCGATTATTGTTTTTTGGGTTGAGGCATTCTTGCATTTATGCTCACCCTATGTAGATTATTCGTACTGTATCTCTCTATTTGTAACCATATTATATTGATTTCGTAATGCATTGCGATTATTGTTTGCAATTGAATAACGCGGCTAATCTTA
ATTTTCAATTGTTCTCCAACCGAGTTAAGAAATTTGCAA