sp|B2LSD2|MUCIN_MUCPR ----> Cysteine proteinase mucunain (Fragment)

Intron 1 GTAATTCTCA ... ATATTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	69.23	2	6	3
A2	55.56	2	4	1
A3	61.54	5	10	6
A4	100	0	5	3
A5	100	0	1	0
A6	81.44	7	37.4	24
A7	64.27	27.7	60	39.7
A8	71.28	22.7	61	51.7
A9	77.33	12.7	40	46.7
A10	78.18	6	33	10

>TRINITY_DN47773_c2_g5_i2 from sample A7 / Example spliced isoform

GGTGTACGTACATCCACCACCGGGAGACATGTTATCCTCGCGTTCCCATTTGGTTGTTGGGGTTTCATTACTTGTCCATTATCATGAGTCCCTGCAACCGTGAGCCCAGGTTGGAGGCCGACAAATGCGATCTGTGCTCGCTGGACTGAGAATAGTCATCTTTGTGGTCTGAATACTGGCTGTCCAAATCACCTTTGAGA
CACATATGAGATTTGGCCTTTTGGGTAAGGTAAGAATTTGTTGATTGAGCGAAGGGGGTTTGGTTGTTTTTGAGAGAGGTCCAAGGTTTTGAACCCGCAACAATCAGAATGGTGGTTGAAACATTGTCGTCGGGGAGTATTCAGTACATTAGGACGAGGCATCAATCCTAGAGCTCCAACGATGGCACTCCCTTGCATTC
CAGGGCCGTTACTGTGAGTGTCGAAGACTTGAAAACGATGGCATGATTTTTCTTTGAATTGGTGGATTGTGATACTTGTTACAACCTAGGATGCAATGGCAAACTCATGGATTATGCTTTCCAGTTTATCATTAGCAATGGTGGGCTTGACGGCCAAGATGATTACCATTACAAGGCCACCAATGGCAGCTGTGACGCTT
ATAGAGTGGTTGGTCTTGAGTTCTTCTCTGGTACTGTGGTACATGGACATAGATTGTAATTTTAGTCATTGGATTTTTCTCCTGCCTGTGTTATGTAAGGTTAAGGTAAGTTTTTTTGTTTTTTATTTTTTATTTACTGATTCAATTCCCAAGATTTTCCTGAGTTCAAGTTAACTATTTTTCTTGTCTCACTAAGTTGT
TCTCCAAGACTAATATGGAAACGTTGCAGCTAGTTCACAATAGAACCTTTGAAAAATCCAACTCCCATAAGTAGCGTTGCTACCTTTAGAAGTATATATTTTCAGCTATTGGCCAGAGAA

>TRINITY_DN47773_c2_g5_i3 from sample A7 / Example unspliced isoform:

GGTAGCCATATTGTAGTCCAGTTGCTTCGTGATAACAACATAATTCCAAGCACCATGCACAATTTGTTCTCCGTTCCTTGCTGTTTTATTTTAGAAGTGACATTTTTAACGTGTTGCTCTTTATTGAAAGGAAGTGTTATTTAACCAACTGAACATTCTGATAAAACTAAAATATCACTCTTTTAGCTTGATCATATTTT
TGAGAAATTTCATTCTCTATCTAAAACAATAGTTTCTTAACAACATATTCCATGCACAACAACCCTTACAGGTTTCCTCCATTTAGCAAAACGATATTTGCATAAAACAACAATGAAGAAGAAGCCACAATAAAAATAGATGAACTCTACAGCCAAGTTCAAATAAAAATAAAAATATGTTTGTCATAATGGTGTTGGTG
CCAAGACGAAATTGAGATCTCAAAGCTCACAATAGCAGTAGCAACAACATCATCATGGCAAAGACTAATTGAGCAGGGCAATGCCAACGATGCAACAAGTTGTAGATGGAAGAATAGAGATGGATTTGAATGACTCCATGCTTTCAGGTAGAGGAGGAAATATGGTTCCTTCTGACATGATAGAGCAAGAGCAAGACCTA
AATGGTAGATGGAAAGTGCGCTCCAATGACAACTAAGGAAATGAGTTGGTTGGAGTTTGGGAATTTCAGCCAAAGTACTTTGCATACATTTAACTGTTTGTTTGATGCTTGTATTTGACACTTTTCTATAGATTTTTCATAGGAGATTGTTTGAACTGTGAAGAGTTACATGTTTCTATTATTTTTCACCCCAAGGCTCT
TCTTTTGTATTGGGGAATTGTTGTTGTTTAAATTTGCATTCAAATTATTTAGGAAGACATTTTTTCATTCACATTGAAATATGTTAATATACATATGATGGACTATATCATTGGAATTCTATCCGACTAACTGTGATTGCATAAGTTATTTAAAACATGCTTGTTAATCATTCTTGTTAACATTGAAAAATCGCGATGGA
TACACAAGGAAAACCTACAAGGCGATAGGATATACGTATTGAGATGGACCCTACAATTTTGTAGCGCTCCTTTAACAATTGGAGTCTTCTTTCCTCTGTGTTTCCTAGATATTGCTCAGCTCCTTCCTTTTTCTACGTAAAGTCAAGACACCCAACTATGCTTTGTAAGAATATCCCAGTTCTTAAATCCAGCACCCGTG
TGAAGAAAATCAAGCAACAATAGCAAAGGATGAGTTTATTGAGTAGAATTTAAGCAAGAAGAATAACACTATTCCAAAAATACTCTGAATCCAATTGAAAACTAATTTCACTTATTATATAAGTAACATAATAATAACTAAAAACCATAATAAAGCAACTTCATAAAAACAAATATTTAGAAAGAACATAATAATAACTA
AAAAACCATCACCATAAGATGATAACGTGCAAGCACCTTTCCTAAAGCGCTCATGCTTTCGGCGTGGTACACTTGCTTTTGATTGGCAGCTATTGAAGGCATCAATCAAATCATGACTTACATTTTGGCTTCGCTGGTCGAGCAGGAATTGGTGGATTGTGATACTTGTTACAACCTAGGATGCAATGGCAAACTCATGG
ATTATGCTTTCCAGTTTATCATTAGCAATGGTGGGCTTGACGGCCAAGATGATTACCATTACAAGGCCACCAATGGCAGCTGTGACGCTTATAGAgtaattctcaagtatttttttgtaattgggattcgatcctgccacatgttgtattttcttatttatcaggactgattaatctatatgtgtagaaaaatgcccagc
tgactgctctatcggaactcagctggaacacagtgtgactctagagggatatggctcagagtctggtctaacattctgattaaaatccatattgacagGTGGTTGGTCTTGAGTTCTTCTCTGGTACTGTGGTACATGGACATAGATTGTAATTTTAGTCATTGGATTTTTCTCCTGCCTGTGTTATGTAAGGTTAAGGT
AAGTTTTTTTGTTTTTTATTTTTTATTTACTGATTCAATTCCCAAGATTTTCCTGAGTTCAAGTTAACTATTTTTCTTGTCTCACTAAGTTGTTCTCCAAGACTAATATGGAAACGTTGCAGCTAGTTCACAATAGAACCTTTGAAAAATCCAACTCCCATAAGTAGCGTTGCTACCTTTAGAAGTATATATTTTCAGCT
ATTGGCCAGAGAA

Intron 2 GTAAGTTTGT ... AACTATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	5	10	4
A2	62.5	3	5	5
A3	40	18	12	8
A4	70.59	5	12	5
A5	75	1	3	3
A6	54.95	50	61	12
A7	50	33	33	12
A8	56.25	28	36	4
A9	59.7	27	40	5
A10	49.3	36	35	8

>TRINITY_DN34902_c0_g1_i1 from sample A6 / Example spliced isoform

CGTACATCCACCACCGGGAGACATGTTATCCTCGCGTTCCCATTTGGTTGTTGGGGTTTCATTACTTGTCCATTATCATGAGTCCCTGCAACCGTGAGCCCAGGTTGGAGGCCGACAAATGCGATCTGTGCTCGCTGGACTGAGAATAGTCATCTTTGTGGTCTGAATACTGGCTGTCCAAATCACCTTTGAGACACATA
TGAGATTTGGCCTTTTGGGTAAGGTAAGAATTTGTTGATTGAGCGAAGGGGGTTTGGTTGTTTTTGAGAGAGGTCCAAGGTTTTGAACCCGCAACAATCAGAATGGTGGTTGAAACATTGTCGTCGGGGAGTATTCAGTACATTAGGACGAGGCATCAATCCTAGAGCTCCAACGATGGCACTCCCTTGCATTCCAGGGC
CGTTACTGTGAGTGTCGAAGACTTGAAAACGATGGCATGATTTTTCTTTGGTCAATTGGAGCTTCCATGGGATGTTGGAATTAACTTAATTTAAAATTAAACAACTGTGACCAACTTACGTAGGTTTTTCATTTTTCCGTCTAGTGCATACTATGTAAGGTTTTTCGTTCCTGCAACTACAACCATTCATTTCCCTATTC
AACATGCCTCAACACCCTACTTATAGACTACACACTACTCTTCCCATCTCGTTCCCTTGCTTGCAGACTACCTTCTTCTCGCACTCTCTTGTTTTCTCTCAACTAACACATTTCTTCATAGAATATCGAGGATTAATTGCTAGACTTCCAGTACAATCTCATTATTCTCAAGAAAGGTCCCATAAAATAAAAGCCAATGA
TTTTTCGCAACAGATCTAAGGATTTTTTGGGCAATTTTTGGCTGTCATGTTATTGTTCTTGAAATGCCATAAATATGTTGGTGGGTATCTATTACTAATGACATTATGAGCTCTTAGTTATATACTGTACGTAAGGTGCTCGTGGAGTATACTCCATCTGAGATAGAGGGATCCCAAGATAAACATTATCATTCTGCCTA
TTTAAGGACTCATTTTGTTGATTAAATTTGAAATCTCAAGAGAAGACCCTTGGCATATTGGAATGTTCTTGATTTTGCATTTATACCTTGTATGCATCAAGATGATAGGGGATATCCAGAGAAGTTGCATCATGAATGAGAGGAATTAGAAAGATGTCAATGAGAAGGTTTGAATTTAGAAGCTTTGATTGATG

>TRINITY_DN34902_c0_g1_i5 from sample A6 / Example unspliced isoform:

CGTACATCCACCACCGGGAGACATGTTATCCTCGCGTTCCCATTTGGTTGTTGGGGTTTCATTACTTGTCCATTATCATGAGTCCCTGCAACCGTGAGCCCAGGTTGGAGGCCGACAAATGCGATCTGTGCTCGCTGGACTGAGAATAGTCATCTTTGTGGTCTGAATACTGGCTGTCCAAATCACCTTTGAGACACATA
TGAGATTTGGCCTTTTGGGTAAGGTAAGAATTTGTTGATTGAGCGAAGGGGGTTTGGTTGTTTTTGAGAGAGGTCCAAGGTTTTGAACCCGCAACAATCAGAATGGTGGTTGAAACATTGTCGTCGGGGAGTATTCAGTACATTAGGACGAGGCATCAATCCTAGAGCTCCAACGATGGCACTCCCTTGCATTCCAGGGC
CGTTACTGTGAGTGTCGAAGACTTGAAAACGATGGCATGATTTTTCTTTgtaagtttgttcacatattgcttttgattactccattttgtgtcaattcataaacataaaatctggttagagcactggctatgatatctatatatgacctcggtactaactattcagGGTCAATTGGAGCTTCCATGGGATGTTGGAATTA
ACTTAATTTAAAATTAAACAACTGTGACCAACTTACGTAGGTTTTTCATTTTTCCGTCTAGTGCATACTATGTAAGGTTTTTCGTTCCTGCAACTACAACCATTCATTTCCCTATTCAACATGCCTCAACACCCTACTTATAGACTACACACTACTCTTCCCATCTCGTTCCCTTGCTTGCAGACTACCTTCTTCTCGCA
CTCTCTTGTTTTCTCTCAACTAACACATTTCTTCATAGAATATCGAGGATTAATTGCTAGACTTCCAGTACAATCTCATTATTCTCAAGAAAGGTCCCATAAAATAAAAGCCAATGATTTTTCGCAACAGATCTAAGGATTTTTTGGGCAATTTTTGGCTGTCATGTTATTGTTCTTGAAATGCCATAAATATGTTGGTG
GGTATCTATTACTAATGACATTATGAGCTCTTAGTTATATACTGTACGTAAGGTGCTCGTGGAGTATACTCCATCTGAGATAGAGGGATCCCAAGATAAACATTATCATTCTGCCTATTTAAGGACTCATTTTGTTGATTAAATTTGAAATCTCAAGAGAAGACCCTTGGCATATTGGAATGTTCTTGATTTTGCATTTA
TACCTTGTATGCATCAAGATGATAGGGGATATCCAGAGAAGTTGCATCATGAATGAGAGGAATTAGAAAGATGTCAATGAGAAGGTTTGAATTTAGAAGCTTTGATTGATG