sp|Q06827|CATR_SCHDU ----> Caltractin

Intron 1 GTATACTCTT ... TTTTGGTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.98	28.3	78	70
A2	62.47	35.3	67	46
A3	69.7	28.7	62	64
A4	67.73	30.7	64	62
A5	72.24	7	25	11
A6	75.13	79.3	204	264
A7	71.45	135.3	329	328
A8	71.31	134	290	345
A9	72.38	141.7	327	392
A10	70.67	112.7	249	279

>TRINITY_DN114097_c0_g1_i2 from sample A3 / Example spliced isoform

TGCATATGCTTGGTCAAGGTAAATCACAGATTGCATTCTGCACAAAGAGAGTGATGCATTGATTTTAGCTTTATGATGATTTGGATGTTATATGTGGTGCCAGAGCAAGAAAATGAAGTCTCTTAATTCATTAATCGTGGATAAAGCATCCGAAGAGCATGGATGGACCGCTTTACAACAGAATGAACTGGATGAGGACG
CTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCAGAAGCGTCAAAGCAAGATTCAGAAGTTGGGAGACCAAAAACAAAAGTAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAACAGCCAAGGTTGACAAATTTCCCCGTGGCAGAAGACAAAGAAAGCAAACAAAG
AGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGCAGGCAAGGGGAAAATTTCAGTGCGAGACTTGGAGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATGAAATGGTCAGCACCTTTGACAAGGATTGCGATAGACAGCTAAGTCTCGAGGA
CTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAAGTGCATGCACGTGCAGAGAGATATGGCTGCCTGCTGCATGTGTTTTAAGTGTTGCATAAAATCTTAAAGCGGATGTTTTTTGGACCAAAGTGGGAAGATGTAGAAGCA

>TRINITY_DN114097_c0_g1_i4 from sample A3 / Example unspliced isoform:

CATACTTGAGCATACCTTTATAACTACCGGGGTGAAATCTTTCCAATGATACTCACTATATATATCGCGAGATACAAGGTCCATAGATTCTTACGATTCGATTCTTTTCACAATTTATAACTGTCTTCTGGTTTTCAAGATTTCCATGAAAAATTGACCATATTCAGAATTGTAATGGGTTTACCCAAGATCGTGTCGGA
GAATTGAGGTGCGTTATTGCGGGAGCAATGAAGAAGAAGCAGGTTGCTGCCGTGGACGACAGTGATGAAGCAGCTAGTTCTTCAGAACAAGAAGAGAACGAAGAGGGATTGAAGAACGGAGGAGACAAAGGTAAGGCCAAATTGGCTTCTTCCTCTGCAACTATAACAGAATATGAAAAACAGAGACAGCAAAGAATTAA
GGAGAACAGAGCAAAGCTACAAGCCCTTCGCTTGCCCGTACTTGCATCAACCCTCTCAAAAAACAGCAACAAAAAGCTGAAAAAACCCACTGCCCAAAAGGCTAAAACCAAACAGTATGATGACTATCAGCCCTCCGATGAACAGTCCGAGAACTCTAGTGACGAGGACGCCGAATACAAAGTGCCGACGCCGAAGAAGG
TCACCCCTGCAAAACGAAGGCCTCCTGGACGCCCAAAGAGCAAGAAAATGAAGTCTCTTAATTCATTAATCGTGGATAAAGCATCCGAAGAGCATGGATGGACCGCTTTACAACAGAATGAACTGGATGAGGACGCTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCA
GAAGCGTCAAAGCAAGATTCAGAAGTTGGGAGACCAAAAACAAAAGTAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAACAGCCAAGGTTGACAAATTTCCCCGTGGCAGAAGACAAAGAAAGCAAACAAAGAGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGC
AGGCAAGGGGAAAATTTCAGTGCGAGACTTGGAGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATGAAATGGTCAGCACCTTTGACAAGGATTGCGATAGACAGCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAgtatactcttgaaaa
cttgtagttattgctgtaatttatttgagttgtaccctcctccatggcagccatgctcatttgcctctcatttattgtgttttggtgagAGTGCATGCACGTGCAGAGAGATATGGCTGCCTGCTGCATGTGTTTTAAGTGTTGCATAAAATCTTAAAGCGGATGTTTTTTGGACCAAAGTGGGAAGATGTAGAAGCACT
TTTAAATGAGTTTGGCAGATGGGTGTTTGTAAGGGACCCTAAATCCACAGAAATGGATGATTCTTTGTTGATGGATGAATTGCTTGTGTCAAATTTCAATATGCAGATGTGAAAAGACTCAGGACATCTCTGATTGTAGGAGAATCAGTTAGAGATTATGCAGGGTGTTGTATCAAGACAGTATCAAAGAACCATATTTT
ACCACATCAGCTGCATCCTGAATTGTATCTAAGCTTCATCATTATGATTTGTATAAATAAATGGTTGGTCTCGGCATTGCCTTTTCAGCAAGAAGAAATTTGTTCCAGCTTTCCTTCCTGGATGCACTAACAAATATCTTGGCAAAGCTTTCAAGAATGTTAGCACCTGGAAATATTAGGTGCTGACTTTGGTTCTTATG
CCTGATAAATGCAAATCAATACAAGTTTTAACGCCTTTTTGACGTCGTTAGATTTATTCTTCATAATTGTAATCAAAGTGTTTTATAATTGCCGAAATTTGTGGAGCTTGAAAATGAAAAAAAAAA

Intron 2 GTGAAAGATA ... TCTTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.32	112.5	29.5	28
A2	15.1	132	20	27
A3	14.86	96	10.5	23
A4	16.32	73	11.5	17
A5	12.64	38	6	5
A6	13.45	201	27.5	35
A7	12.2	429.5	46	73.5
A8	13.98	336.5	38.5	71
A9	13.11	382.5	46.5	69
A10	11.14	359	41	49

>TRINITY_DN105060_c0_g1_i2 from sample A4 / Example spliced isoform

AGCTTTATGATGATTTGGATGTTATATGTGGTGCCAGAGCAAGAAAATGAAGTCTCTTAATTCTTTAATCGTGGATAAAGCATCCAAAGAGCATGGATGGACGGCTTTACAACAGAATGAACTGGATGAGGACACTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCAG
AAGCGTCAAAGCAAGATTCAGAAGTTGGGAGACCAAAAACAAAAGCAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAACAGCCAAGGTTGACAAATTTCCTCGTGGCAGAAGACAAAGAAAGCAAACAAAGAGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGCA
GGCAAGGGGAAAATTTCAGTGCGAGACTTGGGGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATGAAATGGTCAGCACCTTCGACAAGGATTGCGATGGACAGCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAGTATACTCTTGAAAAC
TTGTAGTTATTGCTGTAATTTATTTGAGTTGTACTCTCCTCCATGGCAGCCATGCTCATCTGCCTCACATTTATTGTGTTTTGGTGAGTTTGCATGCACGTGCAGAGAGATATGGCTGCCTGCTGCATGTGTTTTAAGTGTTGCATAAAATCTTAAAGCGGATGTTTTTTGGACCAAAGTGGGAAGATGTAGAAGCATTT
TAAATGAGTTTGGCAGATGGGTGTTTGTAAGGGACCCTAAATCCACAGAAATGGATGATTCTGTTGATGGATGAATTGCTTGTGTCAAATTTCAATATGCAGATGTGAAAAGACTCAGGACATCTCTGATTGTAGGAGAATCAGTTAGAGATTATGCAGGGTGTTGTATCAAGACAGAGTATCAAAGAACCATATTTTAC
CACATCAGCTGCATCCTGAATTGTATCTAAGCTTCATCATTATGATTTGTATAAATAAATGGTTGGTCTCGGCATTGCCTTTTCAGCAAGAATAAATTTGTTCTAGCTTTCCTTCCTGGATGCACTAACAAATATCTTGGCAAAGCTTTCAAGAATTAGCACCTGGAAATATTAGGTGCTGACTTTGGTTCTTATGCCTG
ATAAATGCAAATCAATACAAGTTCTAACTCCTTTTTGATGTCGTCAAAAA

>TRINITY_DN105060_c0_g1_i3 from sample A4 / Example unspliced isoform:

TCTATGCTCATTGCACAACCAAAATTTTCCTAGTTTCTGATGCAGTTAATGACTCAGAGAGGGGGGGAGGGAAGGAAGCAGGTTGATACATTGGAGGATCTGTACAAGTTTACTTTTAATATCTCTTCCTGTAGAATTTTTTACAAATTCTCTAATGCTGTTTCATGTTTCTGCACCACAGAAGCAGGCAAGGGGAAAAT
TTCAGTGCGAGACTTGGGGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATGAAATGGTCAGCACCTTCGACAAGGATTGCGATGGACAGgtgaaagatatttaaatcttctttccgagactaatttggaaaaccatagtagcattctttttaatcagcgtgcttaaaatattcgtatgctttt
tgaaagagattggctgcagtgaaattaaagatacgctgtttctctcttttgtagCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAGTATACTCTTGAAAACTTGTAGTTATTGCTGTAATTTATTTGAGTTGTACTCTCCTCCATGGCAGCCATGCTCATTTGCCTC
TCATTTATTGTGTTTTGGTGAGAGTGCATGCACGTGCAGAGAGATATGGCTGCCTGCTGCATGTGTTTTAAGTGTTGCATAAAATCTTAAAGCGGATGTTTTTTGGACCAAAGTGGGAAGATGTAGAAGCACTTTTAAATGAGTTTGGCAGATGGGTGTTTGTAAGGGACCCTAAATCCACAGAAATGGATGATTCT

Intron 3 GTATACTCTT ... GCACGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.1	42	78	72
A2	63.33	33	67	47
A3	61.17	40	62	64
A4	65.41	32	64	57
A5	56.76	16	25	17
A6	66.24	118	204	259
A7	67.05	156	329	306
A8	64.64	169	290	328
A9	67.18	170	327	369
A10	65.69	135	249	268

>TRINITY_DN125277_c0_g1_i5 from sample A1 / Example spliced isoform

TAGATTCTTACGATTCGATTCTTTTCACAATTTATAACTGTCTTCTGGTTTTCAAGATTTCCATGAAAAATTGACCATATTCAGAATTGTAATGGGTTTACCCAAGATCGTGTTGGAGAATTGAGGTGCGTTATTGCGGGAGCAATGAAGAAGAAGCAGGTTGCTGCCGTGGACGACAGTGATGAAGCAGCTACTTCTTC
AGAACAAGAAGAGAACGAAGAGGGATTGAAGAACGGAGGAGACAAAGGTAAGGCCAAATTGGCTTCTTCCTCTGCAACTATAACAGAATATGAAAAACAGAGACAACAAAGAATTGAGGAGAACAGAGCAAAGCTACAAGCCCTTCGCTTGCCCGTACTTGCATCAACCCTCTCAAAAAACAGCAACAAAAAGCTGAAAA
AACCCACTGCCCAAAAGGCTAAAACCAAACAGTATGATGACTATCAGCCCTCCGATGAACAGTCCGAGAACTCTAGTGACGAGGACGCCGAATACAAAGTGCCGACGCCGAAGAAGGTCACTCCTGCAAAACGAAAGCCTCCTGGACGCCCAAAGAGCAAGAAAATGAAGTCTCTTAATTCTTTAATCGTGGATAAAGCA
TCCAAAGAGCATGGATGGACGGCTTTACAACAGAATGAACTGGATGAGGACACTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCAGAAGCGTCAAAGCAAGATTCAGAAGTTGGGAGACCAAAAACAAAAGTAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAAC
AGCCAAGGTTGACAAATTTCCTCGTGGCAGAAGACAAAGAAAGCAAACAAAGAGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGCAGGCAAGGGGAAAATTTCAGTGCGAGACTTGGAGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATG
AAATGGTCAGCACCTTTGACAAGGATTGCGATAGACAGCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAAGAGATATGGCTGCCTGCTGCATGTGTTTTAAGTGTTGCATAAAATCTTAAAGCGGATGTTTTTTGGACCAAAGTGGGAAGATGTAGAAGCACTTTTA
AATGAGTTTGGCAGATGGGTGTTTGTAAGGGACCCTAAATCCACAGAAATGGATGATTCTTTGTTGTTGGATGAATTGCTTGTGTCAAATTTCAATATGCAGATGTGAAAAGACTCAGGACATCTCTGATTGTAGGAGAATCAGTTAGAGATTATGCAGGGTGTTGTATCAAGACAGTATCAAAGAACCATATTTTACCA
CATCAGCTGCATCCTGAATTGTATCTAAGCTTCATCATTATGATTTGTATAAATAAATGGTTGGTCTCGGCATTGCCTTTTCAGCAAGAAGAAATTTGTTCCAGCTTTCCTTCCTGGATGCACTAACAAATATCTTGGCAAAGCTTTCAAGAATGTTAGCACCTGGAAATATTAGGTGTTGACTTTGGTTCTTATGTCTG
ATAAATGCAAATCAATACAAGTTTTAACGCCTTTTTGACGTCGTTAGATTTATTCTTCATAATTGTAATCAAAGTGTTTTATACTTGCTGAAATTTGTGGAGCTTGAAAATGTGTTTTCTTGCTGGAAAAGTTTTTATAGGAAGTGGTAG

>TRINITY_DN125277_c0_g1_i3 from sample A1 / Example unspliced isoform:

TAGATTCTTACGATTCGATTCTTTTCACAATTTATAACTGTCTTCTGGTTTTCAAGATTTCCATGAAAAATTGACCATATTCAGAATTGTAATGGGTTTACCCAAGATCGTGTTGGAGAATTGAGGTGCGTTATTGCGGGAGCAATGAAGAAGAAGCAGGTTGCTGCCGTGGACGACAGTGATGAAGCAGCTACTTCTTC
AGAACAAGAAGAGAACGAAGAGGGATTGAAGAACGGAGGAGACAAAGGTAAGGCCAAATTGGCTTCTTCCTCTGCAACTATAACAGAATATGAAAAACAGAGACAACAAAGAATTGAGGAGAACAGAGCAAAGCTACAAGCCCTTCGCTTGCCCGTACTTGCATCAACCCTCTCAAAAAACAGCAACAAAAAGCTGAAAA
AACCCACTGCCCAAAAGGCTAAAACCAAACAGTATGATGACTATCAGCCCTCCGATGAACAGTCCGAGAACTCTAGTGACGAGGACGCCGAATACAAAGTGCCGACGCCGAAGAAGGTCACTCCTGCAAAACGAAAGCCTCCTGGACGCCCAAAGAGCAAGAAAATGAAGTCTCTTAATTCTTTAATCGTGGATAAAGCA
TCCAAAGAGCATGGATGGACGGCTTTACAACAGAATGAACTGGATGAGGACACTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCAGAAGCGTCAAAGCAAGATTCAGAAGTTGGGAGACCAAAAACAAAAGTAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAAC
AGCCAAGGTTGACAAATTTCCTCGTGGCAGAAGACAAAGAAAGCAAACAAAGAGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGCAGGCAAGGGGAAAATTTCAGTGCGAGACTTGGAGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATG
AAATGGTCAGCACCTTTGACAAGGATTGCGATAGACAGCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAgtatactcttgaaaacttgtagttattgctgtaatttatttgagttgtactctcctccatggcagccatgctcatttgcctctcatttattgtgtttt
ggtgagagtgcatgcacgtgcagAGAGATATGGCTGCCTGCTGCATGTGTTTTAAGTGTTGCATAAAATCTTAAAGCGGATGTTTTTTGGACCAAAGTGGGAAGATGTAGAAGCACTTTTAAATGAGTTTGGCAGATGGGTGTTTGTAAGGGACCCTAAATCCACAGAAATGGATGATTCTTTGTTGTTGGATGAATTGC
TTGTGTCAAATTTCAATATGCAGATGTGAAAAGACTCAGGACATCTCTGATTGTAGGAGAATCAGTTAGAGATTATGCAGGGTGTTGTATCAAGACAGTATCAAAGAACCATATTTTACCACATCAGCTGCATCCTGAATTGTATCTAAGCTTCATCATTATGATTTGTATAAATAAATGGTTGGTCTCGGCATTGCCTT
TTCAGCAAGAAGAAATTTGTTCCAGCTTTCCTTCCTGGATGCACTAACAAATATCTTGGCAAAGCTTTCAAGAATGTTAGCACCTGGAAATATTAGGTGTTGACTTTGGTTCTTATGTCTGATAAATGCAAATCAATACAAGTTTTAACGCCTTTTTGACGTCGTTAGATTTATTCTTCATAATTGTAATCAAAGTGTTT
TATACTTGCTGAAATTTGTGGAGCTTGAAAATGTGTTTTCTTGCTGGAAAAGTTTTTATAGGAAGTGGTAG

Intron 4 GTATACTCTT ... CAATATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.36	30	78	96
A2	70.75	37	67	112
A3	77.49	26	62	117
A4	73.96	22	64	61
A5	84.13	5	25	28
A6	74.05	160	204	709
A7	76.5	204	330	998
A8	76.99	204	289	1076
A9	78.28	169	328	890
A10	75.64	138	249	608

>TRINITY_DN45897_c0_g1_i5 from sample A7 / Example spliced isoform

CCCACAACAACATACTTGAGCATACATTCCATACCATTATATATACCGGGGTGAAATCTTTCCCATAGACTCTTACGATTCGATTCTATTCACAATTTATAACTGTCTTCTGGTTTTCAAGATTTCCATGAAAAATTGACCATATTCAGAATTGTAATGGGTTTACCCAAGATCGTGTCGGAGAATTGAGGTGCGTTATT
GCGGGAGCAATGAAGAAGAAGCAGGTTGCTGCCGTGGACGACAGTGATGAAGCAGCTACTTCTTCAGAACAAGAAGAGAACGAAGAGGGATTGAAGAACGGAGGAGACAAAGGTAAGGCCAAATTGGCTTCTTCCTCTGCAACTATAACAGAATATGAAAAACAGAGACAGCAAAGAATTAAGGAGAACAGAGCAAAGCT
ACAAGCCCTTCGCTTGCCCGTACTTGCATCAACCCTCTCAAAAAACAGCAACAAAAAGCTGAAAAAACCCACTGCCCAAAAGGCTAAAACCAAACAGTATGATGACTATCAGCCCTCCGATGAACAGTCCGAGAACTCTAGTGATGAGGACGCCGAATACAAAGTGCCGACGCCGAAGAAGGTCACCCCTGCAAAACGAA
GGCCTCCTGGACGCCCAAAGAGCAAGAAAATGAAGTCTCTTAATTCATTAATCGTGGATAAAGCATCCGAAGAGCATGGATGGACCGCTTTACAACAGAATGAACTGGATGAGGACGCTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCAGAAGCGTCAAAGCAAGAT
TCAGAAGTTGGGAGACCAAAAACAAAAGCAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAACAGCCAAGGTTGACAAATTTCCCCGTGGCAGAAGACAAAGAAAGCAAACAAAGAGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGCAGGCAAGGGGAAAATTTC
AGTGCGAGACTTGGAGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATGAAATGGTCAGCACCTTCGACAAGGATTGCGATGGACAGCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAATGTGAAAAGACTCAGGACATCTCTGATTGTAG
GAGAATCAGTTAGAGATTATGCAGGGTGTTGTATCAAGACAGAGTATCAAAGAACCATATTTTACCACATCAGCTGCATCCTGAATTGTATCTAAGCTTCATCATTATGATTTGTATAAATAAATGGTTGGTCTCGGCATTGCCTTTTCAGCAAGAATAAATTTGTTCTAGCTTTCCTTCCTGGATGCACTAACAAATAT
CTTGGCAAAGCTTTCAAGAATTAGCACCTGGAAATATTAGGTGCTGACTTTGGTTCTTATGCCTGATAAATGCAAATCAATACAAGTTCTAACTCCTTTTTGATGTCGTCAGATTTATTCTTCTTAATTGTAATCAAAGTGTTTTATACTTGCTGAAATTTGTGGAGCTTGAAAATGTGTTTTCTTGCTGGAAAAGTTTT
TATAGGAAGTGGTAGCAGTAGTTGATGCATATGCTCACGTGGATTCCATGCCCAAATTTGTTGAGCTTGAAAATGTATTTTCTTGCTGGAAAAGTTTTTATAAGAAGTGGTAGAGGTAGTTGATGATATGCTCACATGGATTCCATGTATCTCATAACATTCATCCATTCCTTTGTAAGTGTGGATCAACATTTTTCGTG
TAAATTTGATAGCGTCAATGCACATTAAATAAATCCTAG

>TRINITY_DN45897_c0_g1_i3 from sample A7 / Example unspliced isoform:

CCCACAACAACATACTTGAGCATACATTCCATACCATTATATATACCGGGGTGAAATCTTTCCCATAGACTCTTACGATTCGATTCTATTCACAATTTATAACTGTCTTCTGGTTTTCAAGATTTCCATGAAAAATTGACCATATTCAGAATTGTAATGGGTTTACCCAAGATCGTGTCGGAGAATTGAGGTGCGTTATT
GCGGGAGCAATGAAGAAGAAGCAGGTTGCTGCCGTGGACGACAGTGATGAAGCAGCTACTTCTTCAGAACAAGAAGAGAACGAAGAGGGATTGAAGAACGGAGGAGACAAAGGTAAGGCCAAATTGGCTTCTTCCTCTGCAACTATAACAGAATATGAAAAACAGAGACAGCAAAGAATTAAGGAGAACAGAGCAAAGCT
ACAAGCCCTTCGCTTGCCCGTACTTGCATCAACCCTCTCAAAAAACAGCAACAAAAAGCTGAAAAAACCCACTGCCCAAAAGGCTAAAACCAAACAGTATGATGACTATCAGCCCTCCGATGAACAGTCCGAGAACTCTAGTGATGAGGACGCCGAATACAAAGTGCCGACGCCGAAGAAGGTCACCCCTGCAAAACGAA
GGCCTCCTGGACGCCCAAAGAGCAAGAAAATGAAGTCTCTTAATTCATTAATCGTGGATAAAGCATCCGAAGAGCATGGATGGACCGCTTTACAACAGAATGAACTGGATGAGGACGCTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCAGAAGCGTCAAAGCAAGAT
TCAGAAGTTGGGAGACCAAAAACAAAAGCAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAACAGCCAAGGTTGACAAATTTCCCCGTGGCAGAAGACAAAGAAAGCAAACAAAGAGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGCAGGCAAGGGGAAAATTTC
AGTGCGAGACTTGGAGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATGAAATGGTCAGCACCTTCGACAAGGATTGCGATGGACAGCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAgtatactcttgaaaacttgtagttattgctgta
atttatttgagttgtactctcctccatggcagccatgctcatctgcctcacatttattgtgttttggtgagtttgcatgcacgtgcagagagatatggctgcctgctgcatgtgttttaagtgttgcataaaatcttaaagcggatgttttttggaccaaagtgggaagatgtagaagcattttaaatgagtttggcaga
tgggtgtttgtaagggaccctaaatccacagaaatggatgattctgttgatggatgaattgcttgtgtcaaatttcaatatgcagATGTGAAAAGACTCAGGACATCTCTGATTGTAGGAGAATCAGTTAGAGATTATGCAGGGTGTTGTATCAAGACAGAGTATCAAAGAACCATATTTTACCACATCAGCTGCATCCT
GAATTGTATCTAAGCTTCATCATTATGATTTGTATAAATAAATGGTTGGTCTCGGCATTGCCTTTTCAGCAAGAATAAATTTGTTCTAGCTTTCCTTCCTGGATGCACTAACAAATATCTTGGCAAAGCTTTCAAGAATTAGCACCTGGAAATATTAGGTGCTGACTTTGGTTCTTATGCCTGATAAATGCAAATCAATA
CAAGTTCTAACTCCTTTTTGATGTCGTCAGATTTATTCTTCTTAATTGTAATCAAAGTGTTTTATACTTGCTGAAATTTGTGGAGCTTGAAAATGTGTTTTCTTGCTGGAAAAGTTTTTATAGGAAGTGGTAGCAGTAGTTGATGCATATGCTCACGTGGATTCCATGCCCAAATTTGTTGAGCTTGAAAATGTATTTTC
TTGCTGGAAAAGTTTTTATAAGAAGTGGTAGAGGTAGTTGATGATATGCTCACATGGATTCCATGTATCTCATAACATTCATCCATTCCTTTGTAAGTGTGGATCAACATTTTTCGTGTAAATTTGATAGCGTCAATGCACATTAAATAAATCCTAG

Intron 5 GTATACTCTT ... CAATATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.36	30	78	96
A2	70.2	38	67	112
A3	74.9	30	62	117
A4	74.85	21	64	61
A5	84.13	5	25	28
A6	74.05	160	204	709
A7	76.76	201	330	998
A8	77.22	201	289	1074
A9	77.83	173	328	887
A10	75.77	137	249	608

>TRINITY_DN43481_c0_g1_i6 from sample A6 / Example spliced isoform

CAACAACATACTTGAGCATACATTCCATACCATTATATATACCGGGGTGAAATCTTTCCCATAGACTCTTACGATTCGATTCTATTCACAATTTATAACTGTCTTCTGGTTTTCAAGATTTCCATGAAAAATTGACCATATTCAGAATTGTAATGGGTTTACCCAAGATCGTGTTGGAGAATTGAGGTGCGTTATTGCGG
GAGCAATGAAGAAGAAGCAGGTTGCTGCCGTGGACGACAGTGATGAAGCAGCTACTTCTTCAGAACAAGAAGAGAACGAAGAGGGATTGAAGAACGGAGGAGACAAAGGTAAGGCCAAATTGGCTTCTTCCTCTGCAACTATAACAGAATATGAAAAACAGAGACAGCAAAGAATTAAGGAGAACAGAGCAAAGCTACAA
GCCCTTCGCTTGCCCGTACTTGCATCAACCCTCTCAAAAAACAGCAACAAAAAGCTGAAAAAACCCACTGCCCAAAAGGCTAAAACCAAACAGTATGATGACTATCAGCCCTCCGATGAACAGTCCGAGAACTCTAGTGATGAGGACGCCGAATACAAAGTGCCGACGCCGAAGAAGGTCACCCCTGCAAAACGAAGGCC
TCCTGGACGCCCAAAGAGCAAGAAAATGAAGTCTCTTAATTCATTAATCGTGGATAAAGCATCCGAAGAGCATGGATGGACCGCTTTACAACAGAATGAACTGGATGAGGACACTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCAGAAGCGTCAAAGCAAGATTCAG
AAGTTGGGAGACCAAAAACAAAAGCAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAACAGCCAAGGTTGACAAATTTCCTCGTGGCAGAAGACAAAGAAAGCAAACAAAGAGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGCAGGCAAGGGGAAAATTTCAGTG
CGAGACTTGGAGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATGAAATGGTCAGCACCTTCGACAAGGATTGCGATGGACAGCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAATGTGAAAAGACTCAGGACATCTCTGATTCTAGGAGA
ATCAGTTAGAGATTATGCAGGGTGTTGTATCAAGACAGTATCAAAGAACCATATTTTACCACATCAGCTGCATCCTGAATTGTATCTAAGCTTCATCATTATGATTTGTATAAATAAATGGTTGGTCTCGGCATTGCCTTTTCAGCAAGAAGAAATTTGTTCCAGCTTTCCTTCCTGGATGCACTAACAAATATCTTGGC
AAAGCTTTCAAGAATGTTAGCACCTGGAAATATTAGGTGTTGACTTTGGTTCTTATGTCTGATAAATGCAAATCAATACAAGTTTTAACGCCTTTTTGACGTCGTTAGATTTATTCTTCATAATTGTAATCAAAGTGTTTTATAATTGCCGAAATTTGTGGAGCTTGAAAATGTGTTTTCTTGCTGGAAAAGTTTTTATA
GGAAGTGGTAGCAGTAGTTGATGCATATGCTCACGTGGATTCCATGCCCAAATTTGTTGAGCTTGAAAATGTATTTTCTTGCTGGAAAAGTTTTTATAAGAAGTGGTAGAGGTAGTTGATGATATGCTCACATGGATTCCATGTATCTCATAACATTCATCCATTCCTTTGTAAGTGTGGATCAACATTTTTCGTGTAAA
TTTG

>TRINITY_DN43481_c0_g1_i3 from sample A6 / Example unspliced isoform:

CAACAACATACTTGAGCATACATTCCATACCATTATATATACCGGGGTGAAATCTTTCCCATAGACTCTTACGATTCGATTCTATTCACAATTTATAACTGTCTTCTGGTTTTCAAGATTTCCATGAAAAATTGACCATATTCAGAATTGTAATGGGTTTACCCAAGATCGTGTTGGAGAATTGAGGTGCGTTATTGCGG
GAGCAATGAAGAAGAAGCAGGTTGCTGCCGTGGACGACAGTGATGAAGCAGCTACTTCTTCAGAACAAGAAGAGAACGAAGAGGGATTGAAGAACGGAGGAGACAAAGGTAAGGCCAAATTGGCTTCTTCCTCTGCAACTATAACAGAATATGAAAAACAGAGACAGCAAAGAATTAAGGAGAACAGAGCAAAGCTACAA
GCCCTTCGCTTGCCCGTACTTGCATCAACCCTCTCAAAAAACAGCAACAAAAAGCTGAAAAAACCCACTGCCCAAAAGGCTAAAACCAAACAGTATGATGACTATCAGCCCTCCGATGAACAGTCCGAGAACTCTAGTGATGAGGACGCCGAATACAAAGTGCCGACGCCGAAGAAGGTCACCCCTGCAAAACGAAGGCC
TCCTGGACGCCCAAAGAGCAAGAAAATGAAGTCTCTTAATTCATTAATCGTGGATAAAGCATCCGAAGAGCATGGATGGACCGCTTTACAACAGAATGAACTGGATGAGGACACTGCTTTACAACAGGCTCTGGCACTTTCCATGGGTGTTTCTATGGAAGATGCAACTGCTATAGCAGAAGCGTCAAAGCAAGATTCAG
AAGTTGGGAGACCAAAAACAAAAGCAGACAATTCTGAGCTTGGTGGAAAGGCACAGGAAACAGCCAAGGTTGACAAATTTCCTCGTGGCAGAAGACAAAGAAAGCAAACAAAGAGCATGAACACAATACAGCTATCTGAAGATGAAGTTCTAGCATATTTCTTTTTATTCGATGAAGCAGGCAAGGGGAAAATTTCAGTG
CGAGACTTGGAGAGAGTTGCACTGACCCATGATTTTACATGGAGCAAGCAGGAGTTAAATGAAATGGTCAGCACCTTCGACAAGGATTGCGATGGACAGCTAAGTCTCGAGGACTTCCAGACAATAGTTGCTCGATGCAATATGATTGGTGGCTCTTAAGCAAgtatactcttgaaaacttgtagttattgctgtaattt
atttgagttgtaccctcctccatggcagccatgctcatttgcctctcatttattgtgttttggtgagagtgcatgcacgtgcagagagatatggctgcctgctgcatgtgttttaagtgttgcataaaatcttaaagcggatgttttttggaccaaagtgggaagatgtagaagcacttttaaatgagtttggcagatgg
gtgtttgtaagggaccctaaatccacagaaatggatgattctttgttgatggatgaattgcttgtgtcaaatttcaatatgcagATGTGAAAAGACTCAGGACATCTCTGATTCTAGGAGAATCAGTTAGAGATTATGCAGGGTGTTGTATCAAGACAGTATCAAAGAACCATATTTTACCACATCAGCTGCATCCTGAA
TTGTATCTAAGCTTCATCATTATGATTTGTATAAATAAATGGTTGGTCTCGGCATTGCCTTTTCAGCAAGAAGAAATTTGTTCCAGCTTTCCTTCCTGGATGCACTAACAAATATCTTGGCAAAGCTTTCAAGAATGTTAGCACCTGGAAATATTAGGTGTTGACTTTGGTTCTTATGTCTGATAAATGCAAATCAATAC
AAGTTTTAACGCCTTTTTGACGTCGTTAGATTTATTCTTCATAATTGTAATCAAAGTGTTTTATAATTGCCGAAATTTGTGGAGCTTGAAAATGTGTTTTCTTGCTGGAAAAGTTTTTATAGGAAGTGGTAGCAGTAGTTGATGCATATGCTCACGTGGATTCCATGCCCAAATTTGTTGAGCTTGAAAATGTATTTTCT
TGCTGGAAAAGTTTTTATAAGAAGTGGTAGAGGTAGTTGATGATATGCTCACATGGATTCCATGTATCTCATAACATTCATCCATTCCTTTGTAAGTGTGGATCAACATTTTTCGTGTAAATTTG