NA ----> NA

Intron 1 GTGTCCTTTT ... TTGTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.56	78	11.7	22.4
A2	9.68	54.3	3.4	5.9
A3	10.77	74.7	13	9.1
A4	7.7	42	7.1	3.6
A5	19.25	17.3	1.4	4.1
A6	11.02	203.6	20.4	26
A7	14.21	259.3	36	44.1
A8	12.9	228.9	23.1	34.7
A9	17.21	255.4	24.9	55.3
A10	13.77	206.7	27.7	34.3

>TRINITY_DN115821_c0_g1_i4 from sample A1 / Example spliced isoform

GGAGCACCGACGCCTCCACAGTCTTACTCCCGTTCATATTTCTTGCGCTCGTCGTTGAAAAATCCCCCAAATTGGTCGTCATCGTTATATTTTCCCTTTATCAATGGAATCCTGAGAGCGATTTTGAGGAATCTCTGAGAACATGACCCCAATTTGAAACGTTATTGCGAAATAATGCAAACTGAAATCTCCGGATCCTG
CTAAGGTTTCTGCGTCGGACTATGGATTTTACAGGCTAAATGATAAAGTACTGGGGTTGTGGCGACCCGAGTGTTTAATAAATGCTGATTCCCAAGGTTATGAGAGCTTGGTTTCGGCATTTTATCGCATGCATGGGTGGTTGTCTTGGAGGCTGTGCAAAACCAACACCGATCATTGCTGTGGATGAGCCCTCGAAGCG
GTTGAAAGTTCAGGGACGAAGCTTGAAGGGACCTAGCTTCTCTGAAGACTTCTGGAGTTCAAGTACAAATGAGATGGACAACAGTACAGTACAGTCACAGAGAAGCGTGTCATCCATAAGTGCATCAAACCAAAATTCTGATTCCCGGGCAGCCAACATAAATACAAATAACACAGAATTTGTTAATCATGCTCTTATTT
TGTGGAATGAAATGAGGCAACAATGGGTTGGAGATCGTCCTCGGAATCAGGCTAGGCAGTCACGTGAACCTGTTTTAAGTTGGAATACAACTTATGAGAGCTTGCTTGGAACCAATAGGCCATTTCCTCAACTCATTCCTCTTTCTGAAATGGTTGAATTTCTTGTAGATGTCTGGGAGCAGGAAGGGCTGTATGATTAA
AAGTCCAATTCCCTGGCTTAGAATTAGATAGACCAAATAATACTGATATTTTTTAAGATCTAATATTGTTGTGGTGTGCCCAGTAACTTTTACCAATTGATACTGTAAATACCAGTTCAATGATTTTGCTGGAAGCAGATTAATCTTGTCTGCATTATTTTAAGGTGATCCTGGATACCAAGGCCTATCAAGCAAACTAA
TCGATCGCTGTTTTTGATGATAGTTTAAAAATCCGCACTAAAGAGAACCAAAGCAAATCTCTTAAGAACAGAATTACAAGAGTGGAAAGATAAAAAGATGCCGGGTTGTAAAGCAGCTCTAATGACTAGTGGGACAGAAAAGATGCATAGTTTTTTGAGAATTCTGAAATCTATATCCCCTAAATTTTGGAGGACAAATG
TTCTCCAACCTGTTAAGGATCTGTGCCTCGACTCTGAACATGTAGTCGTGAAATTGTTTTTTCTTAACCTAATGGAAACAACGGCAGCAAATATATTGAAGCCTGTTATTGACCTGGCTTTCATTTTTTGGGCAAGTATAATCTGGACCTTTTGTCAGTTATAGAAATAGGAATGGTGTATGTTGATATTTTGCATTACT
CTGATAGAAATATATGCAATGTGTTATTTTCTTATGTAGCCATC

>TRINITY_DN115821_c0_g1_i2 from sample A1 / Example unspliced isoform:

GTTAGACAGTGCAAGGGCACAGGTCACTCAAATCTTCATTGGATGAAGATATATCTAGAGTACTTTTTGGTGTATCTCATTGAAAATGACAACAAACATATTGCACATATTTTCCATATTTTGTCTTTATCATGTTAAATTGACCCATCAGATTCATGATCTTGCAGCTCTTATTTTGTGGAATGAAATGAGGCAACAAT
GGGTTGGAGATCGTCCTCGGAATCAGGCTAGGCAGTCACGTGAACCTGTTTTAAGgtgtccttttatggttcatgttgtggaattactttacattttaaaccttacattagtaataagtatatcttcttagaaaaaaaataattgcgcgcttttattcgaacttaatgttgtttctaatgttgtattgttttcagTTGGA
ATACAACTTATGAGAGCTTGCTTGGAACCAATAGGCCATTTCCTCAACTCATTCCTCTTTCTGAAATGGTTGAATTTCTTGTAGATGTCTGGGAGCAGGAAGGGCTGTATGATTAAAAGTCCAATTCCCTGGCTTAGAATTAGATAGACCAAATAATACTGATATTTTTTAAGATCTAATATTGTTGTGGTGTGCCCAGT
AACTTTTACCAATTGATACTGTAAATACCAGTTCAATGATTTTGCTGGAAGCAGATTAATCTTGTCTGCATTATTTTAAGGTGATCCTGGATACCAAGGCCTATCAAGCAAACTAATCGATCGCTGTTTTTGATGATAGTTTAAAAATCCGCACTAAAGAGAACCAAAGCAAATCTCTTAAGAACAGAATTACAAGAGTG
GAAAGATAAAAAGATGCCGGGTTGTAAAGCAGCTCTAATGACTAGTGGGACAGAAAAGATGCATAGTTTTTTGAGAATTCTGAAATCTATATCCCCTAAATTTTGGAGGACAAATGTTCTCCAACCTGTTAAGGATCTGTGCCTCGACTCTGAACATGTAGTCGTGAAATTGTTTTTTCTTAACCTAATGGAAACAACGG
CAGCAAATATATTGAAGCCTGTTATTGACCTGGCTTTCATTTTTTGGGCAAGTATAATCTGGACCTTTTGTCAGTTATAGAAATAGGAATGGTGTATGTTGATATTTTGCATTACTCTGATAGAAATATATGCAATGTGTTATTTTCTTATGTAGCCATC

Intron 2 GTGCGTGTTC ... CTAGGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.37	56	45	48
A2	42.7	53	42	37
A3	39.02	75	53	43
A4	40.91	52	31	41
A5	47.17	14	12	13
A6	41	159	117	104
A7	45.93	236	196	205
A8	44.39	109	85	89
A9	42.47	212	157	156
A10	45.5	197	169	160

>TRINITY_DN38693_c0_g2_i3 from sample A9 / Example spliced isoform

GCCTCCACAGTCTTACTGCCGTTCATATTTCTTGCGCTCGTCGTTGAAAAATCCCCCAAATTGGTCGTCATCGTTATATTTTCCCTTTATCAATGGAATCCTGAGAGCGATTTTGAGGAATCTCTGAGAACATGACCCCAATTTGAAACGTTATTGCGAAATAATGCAAACTGAAATCTCCGGATCCTGCTAAGGTTTCT
GCGTCGGACTATGGATTTTACAGGCTAAATGATAAAGTACTGGGGTTGTGGCGACCCGAGTGTTTAATAAATGCTGATTCCCAAGGTTATGAGAGCTTGGTTTCGGCATTTTATCGCATGCATGGGTGGTTGTCTTGGAGGCTGTGCAAAACCAACACCGATCATTGCTGTGGATGAGCCCTCGAAGCGGTTGAAAGTTC
AGGGACGAAGCTTGAAGGGACCTAGCTTCTCCGAAGACTTCTGGAGTTCAAGTACAAATGAGATGGACAACAGTACAGTACAGTCACAGAGAAGCGTGTCATCCATAAGTGCATCAAACCAAAATTCTGATTCCCGGGCAGCCAACATAAATACAAATAACACAGAATTTGTTAATCATGCTCTTATTTTGTGGAATGAA
ATGAGGCAACAATGGGTTGGAGATCGTCCTCGGAATCAGGCTAGGCAGTCACGTGAACCTGTTTTAAGTTGGAATACAACTTATGAGAGCTTGCTTGGAACCAATAGGCCATTTCCTCAACTCATTCCTCTTTCTGAAATGGTTGAATTTCTTGTAGATGTCTGGGAGCAGGAAGGGCTGTATGATTAAAAGTCCAATTC
CCTGGCTTAGAATTAGATAGACCAAATAATACTGATATTTTTTAAGATCTAATATTGTTGTGGTGTGCCCAGTAACTTTTACCAATTGATACTGTAAATACCAGTTCAATGATTTTGCTGGAAGCAGATTAATCTTGCCTGCATTATTTTAAGGTGATCCTGGATACCAAGGCCTATCAAGCAAACTAATCGATCGCTGT
TTTTGATGATAGTTTACAAATATGCTCTAAAGAGAACCAAAGCAAATCTCTTAAGAACAGAATTACAAGAGTGGAAAGATAAAAAGATGCCGGGTTGTAAAGCAGCTCTAATGACTAGTGGGACAGAAAAGATGCATAGTTTTTTGAGAATTCTGAAATCTATATTATATATATCCCCTAAATTTTGGAGGACAAATGTT
CTCCAACCTGTTAAGGATCTGTGCCTCGACTCTGAACATGTAGTCGTGAAATTGTTTTTTCTTAACCTAATGGAAACAACGGCAGCAAATATATTGAAGCCTGTTATTGACCTGGCTTTCATTTTTTGGGCAAGTATAATCTGGACCTTTTGTCAGTTATAGAAATAGGAATGGTGTATGTTGAAAAA

>TRINITY_DN38693_c0_g2_i2 from sample A9 / Example unspliced isoform:

GCCTCCACAGTCTTACTGCCGTTCATATTTCTTGCGCTCGTCGTTGAAAAATCCCCCAAATTGGTCGTCATCGTTATATTTTCCCTTTATCAATGGAATCCTGAGAGCGATTTTGAGGAATCTCTGAGAACATGACCCCAATTTGAAACGTTATTGCGAAATAATGCAAACTGAAATCTCCGGATCCTGCTAAGGTTTCT
GCGTCGGACTATGGATTTTACAGGCTAAATGATAAAGTACTGGGGTTGTGGCGACCCGAGTGTTTAATAAATGCTGATTCCCAAGGTTATGAGAGCTTGGTTTCGGCATTTTATCGCATGCATGGGgtgcgtgttcgaaaccctaggaacagTGGTTGTCTTGGAGGCTGTGCAAAACCAACACCGATCATTGCTGTGGA
TGAGCCCTCGAAGCGGTTGAAAGTTCAGGGACGAAGCTTGAAGGGACCTAGCTTCTCCGAAGACTTCTGGAGTTCAAGTACAAATGAGATGGACAACAGTACAGTACAGTCACAGAGAAGCGTGTCATCCATAAGTGCATCAAACCAAAATTCTGATTCCCGGGCAGCCAACATAAATACAAATAACACAGAATTTGTTA
ATCATGCTCTTATTTTGTGGAATGAAATGAGGCAACAATGGGTTGGAGATCGTCCTCGGAATCAGGCTAGGCAGTCACGTGAACCTGTTTTAAGGTGTCCTTTTATGGTTCATGTTGTGGAATTACTTTACATTTTAAACCTTACATTAGTAATAAGTATATCTTTTTAGAAAAAAAATAATTGCGCGCTTTTATTCGAA
CTTAATGTTGTTTCTAATGTTGTATTGTTTTCAGTTGGAATACAACTTATGAGAGCTTGCTTGGAACCAATAGGCCATTTCCTCAACTCATTCCTCTTTCTGAAATGGTTGAATTTCTTGTAGATGTCTGGGAGCAGGAAGGGCTGTATGATTAAAAGTCCAATTCCCTGGCTTAGAATTAGATAGACCAAATAATACTG
ATATTTTTTAAGATCTAATATTGTTGTGGTGTGCCCAGTAACTTTTACCAATTGATACTGTAAATACCAGTTCAATGATTTTGCTGGAAGCAGATTAATCTTGCCTGCATTATTTTAAGGTGATCCTGGATACCAAGGCCTATCAAGCAAACTAATCGATCGCTGTTTTTGATGATAGTTTACAAATATGCTCTAAAGAG
AACCAAAGCAAATCTCTTAAGAACAGAATTACAAGAGTGGAAAGATAAAAAGATGCCGGGTTGTAAAGCAGCTCTAATGACTAGTGGGACAGAAAAGATGCATAGTTTTTTGAGAATTCTGAAATCTATATTATATATATCCCCTAAATTTTGGAGGACAAATGTTCTCCAACCTGTTAAGGATCTGTGCCTCGACTCTG
AACATGTAGTCGTGAAATTGTTTTTTCTTAACCTAATGGAAACAACGGCAGCAAATATATTGAAGCCTGTTATTGACCTGGCTTTCATTTTTTGGGCAAGTATAATCTGGACCTTTTGTCAGTTATAGAAATAGGAATGGTGTATGTTGAAAAA

Intron 3 GTAAGTTTAC ... TCTGCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.38	53	2	21
A2	10.87	41	5	5
A3	20	52	2	13
A4	30.77	27	0	12
A5	19.35	25	3	6
A6	15.34	149	2	27
A7	16.44	188	9	37
A8	23.05	217	9	65
A9	16.94	201	8	41
A10	21.16	149	1	40

>TRINITY_DN40846_c0_g2_i2 from sample A8 / Example spliced isoform

AGCACCGACGCCTCCACAGTCTTACTGCCGTTCATATTTCTTGCGCTCGTCGTTGAAAAATCCCCCAAATTGGTCGTCATCGTTATATTTTACCTTTATTAATGGAATCCTGAGAGCGATTTTGAGGAATCTCTGAGAACATGACCCCAATTTGAAACATTATTGCGAAATAATGCAAACTGAAATCTCCGGATCCTGCT
AAGGTTTCTGCGTCGGACTATGGATTTTACAGGCTAAATGATAAAGTACTGGGGTTGTGGCGACCCGAGTGTTTAATAAATGCTGATTCCCAAGGTTATGAGAGCTTGGTTTCGGCATTTTATCGCATGCATGGGTGGTTGTCTTGGAGGCTGTGCAAAACCAACACCGATCATTGCTGTGGATGAGCCCTCGAAGCGGT
TGAAAGTTCAGGGACGAAGCTTGAAGGGACCTAGCTTCTCCGAAGACTTCTGGAGTTCAAGTACAAATGAGATGGACAACAGTACAGTACAGTCACAGAGAAGCGTGTCATCCATAAGTGCATCAAACCAAAATTCTGATTCCCGGGCAGCCAACATAAATACAAATAACACAGAATTTGTTAATCATGCTCTTATTTTG
TGGAATGAAATGAGGCAACAATGGGTTGGAGATCGTCCTCGGAATCAGGCTAGGCAGTCACGTGAACCTGTTTTAAGTTGGAATACAACTTATGAGAGCTTGCTTGGAACCAATAGGCCATTTCCTCAACTCATTCCTCTTTCTGAAATGGTTGAATTTCTTGTAGATGTCTGGGAGCAGGAAGGGCTGTATGATTAAAA
GTCCAATTCCCTGGCTTAGAATTAGATAGACCAAATAATACTGATATTTTTTAAGATCTAATATTGTTGTGGTGTGCCCAGTAACTTTTACCAATTGATACTGTAAATACCAGTTCAATGATTTTGCTGGAAGCAGATTAATCTTGTCTGCATTATTTTAAGGTGATCCTGGATACCAAGGCCTATCAAGCAAACTAATC
GATCGCTGTTTTTGATGATAGTTTACAAATATGCTCTAAAGAGAACCAAAGCAAATCTCTTAAGAACAGAATTACAAGAGTGGAAAGATAAAAAGATGCCGGGTTGTAAAGCAGCTCTAATGACTAGTGGGACAGAAAAGATGCATAGTTTTTTGAGAATTCTGAAATCTATATTATATATATCCCCTAAATTTTGGAGG
ACAAATGTTCTCCAACCTGTTAAGGATCTGTGCCTCGACTCTGAACATGTAGTCGTGAAATTGTTTTTTCTTAACCTAATGGAAACAACGGCAGCAAATATATTGAAGCCTGTTATTGACCTGGCTTTCATTTTTTGGGCAAGTATAATCTGGACCTTTTGTCAGTTATAGAAATAGGAATGGTGTATGTTGATATTTTG

>TRINITY_DN40846_c0_g2_i1 from sample A8 / Example unspliced isoform:

GGGTAATAGAAGCACTGGAAGTTTCAGGATCCGTTTGAAAATTTCAGGAAATGTTTGATTTTTCACAGGATCCGTTTCATAGGACCAGGATACGTTTCCTTCTGTAAGGGATACGTTTCCATGATGGATCCTAGGCGGATCCGATCCAGATACGTATCGGATCCACAGATCCTAGACACTGGGGTGGTGGATCCGGTAAC
AGAGGAGAAACCTTATTTTCATCAAATTTTGCTTCCCTCATAGTGTTCCTCTTGTTTCAGTGTTGAAGTCTTCTGTTAAATTAGCACAAGGCATCATACAACATATCCACTATAGCATGGGGTTTCTTTGGAGTCTGACAGCCAGGGTCAGAGGAACTGGCATAATAACCCTGCTGTGCCAGGAATTACCTGAGCTATCC
CTCTGATTTGAAGGGATCTTAGTACTGAGCTTCCTCTGCCCGAGCAGCAGAACATTTCCTGGGATGCATCCTCATTCCTGAATCAGCCATGCTTGCACAGGATTGAGAAGAGCTGAATGGGGTTGACAATACTGAAACCATATTCAAAGACAGACCACCTGGTGGTATTATAAAATCTGCTTCATTTAAAGACAAGTGAA
TGCTGAGAGGTGGCTTGATAACATGCCACCTTTCTGTGATTGTACCTCTGAATATCCGTGCCACCTACAATTGCTCCATTAAAATGGTTTAGCTATCCTATCATTATAAATTGGAAGGGGGATGGGTGGGGCGGGAAGGTGAGTAATTAGTCCACTAGTTAATTTGTCGTTTGAACCTTCCATTTTGAAGGGAGGCCCAC
TGATAGCAGTAGATGGACACCCTTTATTAAAACAAGTGACTTGGTTGGCCAATTAATGAGCCACCTGGAATGCCGAGCATTTTACCTGAACAAATCTGCATTCTCTAACCTGGTTGGCTAACTATTCAGCCACCTGGTATGCTGAGCATTTTACGCGTCATGAACAAATCTGCATTCTTTTGAAAGCTGAAATGGAGTAC
CTTCACTATAATACAGGACTTTCCCTTTCCCATAATTAACTTTTGGGTTTTCCATTTCGGATTGCTATTACTAATTTTGCCCCACCTCTAAAGATAATTATTCTCATGCCTAAGCTGTTGGCCTCCTTTATACCAAGAAATGAAAACAGATTGATTCTTCTTTGAGTTTTTATGAGGACTCTAGGCACCCCAGAGTACCC
AAAGTACTGTGAGTGTCCCATTGTTGCAGGGGATGCCCCCACGGACTAGGGGACTTCATTTGGAATGTCTCCTAGGGTACTAGGGGACCAGGGGTGTGCAAGGGAGTGTAGGAGACATTCCAAATGAACTCCCCTAGTTCGTGGAGCTTGGGTGGCATAGGGAACCTATCCCTGACTCTTGAGGACCTATCCCTAACTCT
TGAGGACGTGTCCATGTGGAACACTATAAAAGGGCTGTGATTCTGGATGTAGCGGTTTTATGAAGAAAGCATTCAATGTGAGGTTAGACAGTGCAAGGGCACAGGTCACTCAAATCTTCATTGGATGAAGATATATCTAGAATACTTTGTGGTGTATCTCATTGAAAATGACAACAAACATATTGCACATATTTTCCATA
TTTTGTCTTTATCATGTTAAATTGACCCATCAGATTCATGATCTTGCAGCTCTTATTTTGTGGAATGAAATGAGGCAACAATGGGTTGGAGATCGTCCTCGGAATCAGGCTAGGCAGTCACGTGAACCTGTTTTAAGGTGTCCTTTTATGGTTCATGTTGTGGAATTACTTTACATTTTAAACCTTACATTAGTAATAAG
TATATCTTCTTAGAAAAAAAATAATTGCGCGCTTTTATTCGAACTTAATGTTGTTTCTAATGTTGTATTGTTTTCAGTTGGAATACAACTTATGAGAGCTTGCTTGGAACCAATAGGCCATTTCCTCAACTCATTCCTCTTTCTgtaagtttacctggagtatcattttatttaatggccacgataatttatgccaaaat
cctctgtgaaaatgcaattttaggaattgcatgtagtcagtattattcctctttctgtaagtttacctggagtatcattttatttaatggccacgataatttatgccaaaatcctctgtgaaaatgcaattttaggaattgcatgtagtcagtattatggcttgatacagtttgttctgctgcagGAAATGGTTGAATTT
CTTGTAGATGTCTGGGAGCAGGAAGGGCTGTATGATTAAAAGTCCAATTCCCTGGCTTAGAATTAGATAGACCAAATAATACTGATATTTTTTAAGATCTAATATTGTTGTGGTGTGCCCAGTAACTTTTACCAATTGATACTGTAAATACCAGTTCAATGATTTTGCTGGAAGCAGATTAATCTTGTCTGCATTATTTT
AAGGTGATCCTGGATACCAAGGCCTATCAAGCAAACTAATCGATCGCTGTTTTTGATGATAGTTTACAAATATGCTCTAAAGAGAACCAAAGCAAATCTCTTAAGAACAGAATTACAAGAGTGGAAAGATAAAAAGATGCCGGGTTGTAAAGCAGCTCTAATGACTAGTGGGACAGAAAAGATGCATAGTTTTTTGAGAA
TTCTGAAATCTATATTATATATATCCCCTAAATTTTGGAGGACAAATGTTCTCCAACCTGTTAAGGATCTGTGCCTCGACTCTGAACATGTAGTCGTGAAATTGTTTTTTCTTAACCTAATGGAAACAACGGCAGCAAATATATTGAAGCCTGTTATTGACCTGGCTTTCATTTTTTGGGCAAGTATAATCTGGACCTTT
TGTCAGTTATAGAAATAGGAATGGTGTATGTTGATATTTTG