NA ----> NA

Intron 1 GTAACTAATC ... GTGTAAAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.78	1	3	4
A2	100	0	2	1
A3	60	1	2	1
A4	-1	0	0	0
A5	-1	0	0	0
A6	73.91	3	13	4
A7	14.29	3	1	0
A8	100	0	5	1
A9	75	2	6	6
A10	100	0	9	2

>TRINITY_DN34810_c0_g1_i2 from sample A7 / Example spliced isoform

ACGGAAAGGGAGGACCTTCCCTATTTGAGACGGACTCTCCAAAGGTGAGAGGCTGTCCTAGAGTATATACTTATATACACATGATCTGTTAAAAGAAGGGGAAAAAAAGAAAAAGATTCTTGGGCGTGAAGGAGAAAAATCTGCATTGGGAGAGATAGGAAAAAAAGCAGTCTCAGCGATCTGAAAAAAAAAGGAACTAT
TGCAGTGAAAAGGATCGGTAAGCGGTCTTTACTTGACATTGTTAACCGAGTTTTTATTTTGTTTTTTTGTGTTTTATCATAATGAACAACCCTATTTGATCTCTGTTATCACTTTTGTTTGACGGATGGTTCCATTGTTACCTCGCCACTTCTCTACACGAGTGCAATCAACTATTGTAAGATATCGAACCTCTACGTTG
GAGAAGGCTGCCGCCCAGATTTCAAAATATAATCCTCAGCGCTTAGAAGCAGGCGCAGTTAAAAGATACACGTTTTGCATGATCGCCGTGAAGAATTCTTTGCATTTCATATCTCTTTGTTTTTCTGAAAACGTGGTTAAATGCCAT

>TRINITY_DN34810_c0_g1_i1 from sample A7 / Example unspliced isoform:

ACGGAAAGGGAGGACCTTCCCTATTTGAGACGGACTCTCCAAAGGTGAGAGGCTGTCCTAGAGTATATACTTATATACACATGATCTGTTAAAAGAAGGGGAAAAAAAGAAAAAGATTCTTGGGCGTGAAGGAGAAAAATCTGCATTGGGAGAGATAGGAAAAAAAGCAGTCTCAGCGATCTGAAAAAAAAAGGAACTAT
TGCAGTGAAAAGGATCGGTAAGCGGTCTTTACTTGACATTGTTAACCGAGTTTTTATTTTGTTTTTTTGTGTTTTATCATAATGAACAACCCTATTTGATCTCTGTTATCACTTTTGTTTGACGGATGGTTCCATTGTTACCTCGCCACTTCTCTACACGAGTGCAATCAACTATTGTAAGATATCGAACCTCTACGTTG
GAGAAGGCTGgtaactaatcgatgccctaggagcaagcgatctgtgcatccttgttttaaaacactcttttatgtttagcgatttctatttccgtgtatatgctttggggataggacggctctgatagcctgatcctcatatggttttattgtttattttatgaatggtccattcatttgaggtgatgcattgcatccat
tttgacagagtcgatttctgatcttaaaaccgttcgtaacctttgtgtaaagagCCGCCCAGATTTCAAAATATAATCCTCAGCGCTTAGAAGCAGGCGCAGTTAAAAGATACACGTTTTGCATGATCGCCGTGAAGAATTCTTTGCATTTCATATCTCTTTGTTTTTCTGAAAACGTGGTTAAATGCCAT

Intron 2 GTAACTAATC ... ATTTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	3	4
A2	60	1	2	1
A3	100	0	2	0
A4	-1	0	0	0
A5	-1	0	0	0
A6	66.67	5	13	7
A7	50	1	1	1
A8	60	2	5	1
A9	78.95	2	6	9
A10	100	0	9	4

>TRINITY_DN33015_c0_g1_i3 from sample A6 / Example spliced isoform

GAGGGCACCAATACTCGTAAATCCAGCCATTTTCTAAGAGTACTGTCGGCCTCTCTCTCAAAGAAACGCTGAGCCTTAAGGACCTACCTGCCGCTTGGCTTTCTCGACCTCCAGATGGTGACGGAAAGGGAGGACCTTCCCTATTTGAGACGGACTCTCCAAAGGTGAGAGGCTGTCCTAGAGTATATACTTATATACAC
ATGATCTGTTAAAAGAAGGGGAAAAAAAAGAAAAAGATTCTTGGGCGTGAAGGAGAAAAATCTGCATTGGGAGAGATAGGAAAAAAAGCAGTCTCAGCGATCTGAAAAAAAAAGGAACTATTGCAGTGAAAAGGATCGGTAAGCGGTCTTTACTTGACATTGTTAACCGAGTTTTTATTTTGTTTTTTTGTGTTTTATCA
TAATGAACAACCCTATTTGATCTCTGTTATCACTTTTGTTTGACGGATGGTTCCATTGTTACCTCGCCACTTCTCTACACGAGTGCAATCAACTATTGTAAGATATCGAACCTCTACGTTGGAGAAGGCTGAGTCGATTTCTGATCTTAAAACCGTTCGTAACCTTTGTGTAAAGAGCCGCCCAGATTTCAAAATATAAT
CCTCAGCGCTTAGAAGCAGGCGCAGTTAAAAGATACACGTTTTGCATGATCGCCGGG

>TRINITY_DN33015_c0_g1_i2 from sample A6 / Example unspliced isoform:

GAGGGCACCAATACTCGTAAATCCAGCCATTTTCTAAGAGTACTGTCGGCCTCTCTCTCAAAGAAACGCTGAGCCTTAAGGACCTACCTGCCGCTTGGCTTTCTCGACCTCCAGATGGTGACGGAAAGGGAGGACCTTCCCTATTTGAGACGGACTCTCCAAAGGTGAGAGGCTGTCCTAGAGTATATACTTATATACAC
ATGATCTGTTAAAAGAAGGGGAAAAAAAGAAAAAGATTCTTGGGCGTGAAGGAGAAAAATCTGCATTGGGAGAGATAGGAAAAAAAGCAGTCTCAGCGATCTGAAAAAAAAAGGAACTATTGCAGTGAAAAGGATCGGTAAGCGGTCTTTACTTGACATTGTTAACCGAGTTTTTATTTTGTTTTTTTGTGTTTTATCAT
AATGAACAACCCTATTTGATCTCTGTTATCACTTTTGTTTGACGGATGGTTCCATTGTTACCTCGCCACTTCTCTACACGAGTGCAATCAACTATTGTAAGATATCGAACCTCTACGTTGGAGAAGGCTGgtaactaatcgatgccctaggagcaagcgatctgtgcatccttgttttaaaacactcttttatgtttagc
gatttctatttccgtgtatatgctttggggataggacggctctgatagcctgatcctcatatggttttattgtttattttatgaatggtccattcatttgaggtgatgcattgcatccattttgacagAGTCGATTTCTGATCTTAAAACCGTTCGTAACCTTTGTGTAAAGAGCCGCCCAGATTTCAAAATATAATCCT
CAGCGCTTAGAAGCAGGCGCAGTTAAAAGATACACGTTTTGCATGATCGCCGGG