sp|Q94BS2|MET1_ARATH ----> Protein MET1, chloroplastic

Intron 1 GTTTGTTTCT ... CTTTTTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.62	13.3	2.7	7
A2	19.05	12	3	4
A3	51.75	6	6.8	4.3
A4	34.73	7	4.2	1.3
A5	16.04	12	2.3	2.7
A6	15.21	66.2	12.2	26
A7	11.87	65	9	24.7
A8	14.87	28	5	9
A9	10.37	59	7	21.3
A10	7.82	50.7	4.3	19

>TRINITY_DN109120_c0_g1_i3 from sample A1 / Example spliced isoform

CACCTCATCTCATTTGATCAGCAATTGATCCTCTCGTTCAATCAACAAACTATTTTCATTTCCAACTCTGAAGGCAGTTCCTCGCTACTCCTATCAACCCCTCATCAAGCAAATGGCATCCACAGCGATTGTCTCGCCATCTTCATCCAGCCTGCACAGCAGACAACAACCCATTTTATCGCCCAACAATGCAGTGGTTG
CTCCAAATGCTTCAAGAATCCAGACATCATGTTTCTTTGGGATGTCCTTGTTTGAGGCTAAGGGGGGTCTCTCAATCTCCAAAGCATACAATCCCAACACCAACAATTCCAGAACTGGTATGCCACTGGTGAGAGCAAGGACTGCAGGTGCCTCAACGACAATAGAAGCTGACGTCGACAAACCTCTTGGGTTGGCATTA
GGGCAGAAGTCTGGTGGTGGTGTTTTAATCACGGGAATTGAAAATGGTGGTAATGCTGCAAAAGCTGGTTTAAAAGTCGGGGACCAGGTTCTGTACACCAGTAGTTTTTTTGGAGATGAACTTTGGCCAGCTGACAAACTGGGTTTTACCAAAACTGCCATCCAAGCAAAACCAGATTCAGTCTATTTTGTAGTAAGCAG
AGGCGCAGAGGTAGATGTGAAAAGATTGCCGAAAAGACCGGCCCCTCCTCGATTTGGGAGGAAACTAACTGAGGCTCAGAAGGCCCGGGCAACGCACATTTGTCTCGATTGTGGATACATATACACTTTGACTAAACCATTTGATGAGCAGCGGGATGATTTCGTCTGCCCTCAATGCCTAGCACCCAAGAAGCGTTTTG
CAGGGTATGATGTGGAGAAAGGAAGGGCAATAGGTGGTAGTTTGCCTCCGATTGGTGTAATACTTGGATTAGTTGTTGGTGTTGGAGCTGTTGGTGCACTGCTTTTATATGGCCTTCAATAATCGTTTACAAAATGACTCAGTTGGGTACAAAACTAAAATTATTGTTGCTCTGCTTGAGTGGAGATTTAAAAGTTTCAA
AAACGTCTAGATTTGAGGATCAAATAGTGTTCAATTTGCAAATATGAAATGTAATGGGTAGTGGGTTATGCCAGTTTAGTTTTAGCATTGGAGAACCTGTCTCTGATGGACTTGCAAAGATCTATATCAACGCAGCAAAGCTGGATTTGTTGTATTATGAATGAAATTCTGACAATCTACCCCAAAGGGTAACTCTAGTG
TCTATATATGCATATTTTAATTTGTAAAACCAGGCTATGTCATTCCTAAAGATCCAT

>TRINITY_DN109120_c0_g1_i1 from sample A1 / Example unspliced isoform:

TCAGCTTCTATTGTCGTTGAGGCACCTGCAGTCCTTGCTCTCACCAGTGGCATACCAGTTCTGGAATTGTTGGTGTTTTAATCACGgtttgtttctctcaaaagccacaagtctctctttctctctgagttttgagggtatatttgtttgtggacgatgaagattaggcccgtttttttagGGAATTGAAAATGGTGGTA
ATGCTGCAAAAGCTGGTTTAAAAGTCGGGGACCAGGTTCTGTACACCAGTAGTTTTTTTGGAGATGAACTTTGGCCAGCTGACAAACTGGGTTTTACCAAAACTGCCATCCAAGCAAAACCAGATTCAGTCTATTTTGTAGTAAGCAGAGGCGCAGAGGTAGATGTGAAAAGATTGCCGAAAAGACCGGCCCCTCCTCGA
TTTGGGAGGAAACTAACTGAGGCTCAGAAGGCCCGGGCAACGCACATTTGTCTCGATTGTGGATACATATACACTTTGACTAAACCATTTGATGAGCAGCGGGATGATTTCGTCTGCCCTCAATGCCTAGCACCCAAGAAGCGTTTTGCAGGGTATGATGTGGAGAAAGGAAGGGCAATAGGTGGTAGTTTGCCTCCGAT
TGGTGTAATACTTGGATTAGTTGTTGGTGTTGGAGCTGTTGGTGCACTGCTTTTATATGGCCTTCAATAATCGTTTACAAAATGACTCAGTTGGGTACAAAACTAAAATTATTGTTGCTCTGCTTGAGTGGAGATTTAAAAGTTTCAAAAACGTCTAGATTTGAGGATCAAATAGTGTTCAATTTGCAAATATGAAATGT
AATGGGTAGTGGGTTATGCCAGTTTAGTTTTAGCATTGGAGAACCTGTCTCTGATGGACTTGCAAAGATCTATATCAACGCAGCAAAGCTGGATTTGTTGTATTATGAATGAAATTCTGACAATCTACCCCAAAGGGTAACTCTAGTGTCTATATATGCATATTTTAATTTGTAAAACCAGGCTATGTCATTCCTAAAGA
TCCAT

Intron 2 GCAAACTCTG ... ATGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.65	14	0	3
A2	20	12	0	3
A3	22.22	14	0	4
A4	0	8	0	0
A5	27.27	8	0	3
A6	11.94	118	0	16
A7	7.69	84	0	7
A8	13.75	69	1.5	11
A9	12.82	102	0.5	15
A10	7.69	72	0	6

>TRINITY_DN38017_c0_g1_i2 from sample A8 / Example spliced isoform

CCACCTCATCTCATTTGATCAGCAATTGATCCTCTCGTTCAATCAACAAACTATTTTCATTTCCAACTCTGAAGGCAGTTCCTCGCTACTCCTATCAACCCCTCATCAAGCAAATGGCATCCACAGCGATTGTCTCGCCATCTTCATCCAGCCTGCACAGCAGACAACAACCCATTTTATCGCCCAACAATGCAGTGGTT
GCTCCAAATGCTTCAAGAATCCAGACATCATGTTTCTTTGGGATGTCCTTGTTTGAGGCTAAGGGGGGTCTCTCAATCTCCAAAGCATACAATCCCAACACCAACAATTCCAGAACTGGTATGCCACTGGTGAGAGCAAGGACTGCAGGTGCCTCAACGACAATAGAAGCTGACGTCGACAAACCTCTTGGGTTGGCATT
AGGGCAGAAGTCTGGTGGTGGTGTTTTAATCACGGGAATTGAAAATGGTGGTAATGCTGCAAAAGCTGGTTTAAAAGTCGGGGACCAGGTTCTGTACACCAGTAGTTTTTTTGGAGATGAACTTTGGCCAGCTGACAAACTGGGTTTTACCAAAACTGCCATCCAAGCAAAACCAGATTCAGTCTATTTTGTAGTAAGCA
GAGGCGCAGAGGTAGATGTGAAAAGATTGCCGAAAAGACCGGCCCCTCCTCGATTTGGGAGGAAACTAACTGAGGCTCAGAAGGCCCGGGCAACGCACATTTGTCTCGATTGTGGATACATATACACTTTGACTAAACCATTTGATGAGCAGCGGGATGATTTCGTCTGCCCTCAATGCCTAGCACCCAAGAAGCGTTTT
GCAGGGTATGATGTGGAGAAAGGAAGGGCAATAGGTGGTAGTTTGCCTCCGATTGGTGTAATACTTGGATTAGTTGTTGGTGTTGGAGCTGTTGGTGCACTGCTTTTATATGGCCTTCAATAATCGTTTACAAAATGACTCAGTTGGGTACAAAACTAAAATTATTGTTGCTCTGCTTGAGTGGAGATTTAAAAGTTTCA
AAAACGTCTAGATTTGAGGATCAAATAGTGTTCAATTTGCAAATATGAAATGTAATGGGTAGTGGGTTATGCCAGTTTAGTTTTAGCATTGGAGAACCTGTCTCTGATGGACTTGCAAAGATCTATATCAACGCAGCAAAGCTGGATTTGTTGTATTATGAATGAAATTCTGACAATCTACCCCAAAGGGTAACTCTAGT
GTCTATATATGCATATTTTAATTTGTAAAACCAGGCTATGTCATTCCTAAAGATCCATGTAGCAATCGCAGGATGTCTTAAAAGTTAAAAGGTATGTGACAAATTTGACATTTCAACAACTCCTTGGTAATGTAATTCTTTGAC

>TRINITY_DN38017_c0_g1_i4 from sample A8 / Example unspliced isoform:

TCGATTGTGGATACATATACACTATGACTAAACCATTTGATGAGCAGgcaaactctggcctctgctattgataatgttttgaattttgagtacatccatagaagttcgatgtgttcatgttgatagcattgatattgtaatcaaccttcttgttcttgttgttaatgtttgcagCGGGATGATTTCGTCTGCCCTCAATG
CCTAGCACCCAAGAAGCGTTTTGCAGGGTATGATGTGGAGAAAGGAAGGGCAATAGGTGGTAGTTTGCCTCCGATTGGTGTAATACTTGGATTAGTTGTTGGTGTTGGAGCTGTTGGTGCACTGCTTTTATATGGCCTTCAATAATCGTTTACAAAATGACTCAGTTGGGTACAAAACTAAAATTATTGTTGCTCTGCTT
GAGTGGAGATTTAAAAGTTTCAAAAACGTCTAGATTTGAGGATCAAATAGTGTTCAATTTGCAAATATGAAATGTAATGGGTAGTGGGTTATGCCAGTTTAGTTTTAGCATTGGAGAACCTGTCTCTGATGGACTTGCAAAGATCTATATCAACGCAGCAAAGCTGGATTTGTTGTATTATGAATGAAATTCTGACAATC
TACCCCAAAGGGTAACTCTAGTGTCTATATATGCATATTTTAATTTGTAAAACCAGGCTATGTCATTCCTAAAGATCCATGTAGCAATCGCAGGATGTCTTAAAAGTTAAAAGGTATGTGACAAATTTGACATTTCAACAACTCCTTGGTAATGTAATTCTTTGAC