sp|Q9SIU2|NCBP1_ARATH ----> Nuclear cap-binding protein subunit 1

Intron 1 GTAAGTATGG ... TTACCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	65.5	0	0
A2	4.8	59.5	5	1
A3	3.54	95.5	3	4
A4	3.1	47	2	1
A5	8	34.5	4	2
A6	0	15	0	0
A7	8.43	43.5	2	6
A8	0	6	0	0
A9	3	32.5	2	0
A10	0	38.5	0	0

>TRINITY_DN105617_c0_g1_i6 from sample A5 / Example spliced isoform

CACTAACTTCCTACTTGTTTGATTAAACCAGAAAAGTCCATTAATTCTCTGTTGCAAGAGAGGGCGAGCCCTTCAATGGCAATCGGCAACCCCTTTTAAAAATTTGCCGCCAGTGCGAATAAGATTAAGGCAGAGCATTACCGAGTCTCTGAGAAACGAAGGCGTTGCAGGTGAAGCTGAAGCCTTGTAGAAGCCATTGC
ACAGCATACATTTTTATTAAATAGGAATAAGAAGAAACCATGAATAGCTGGCGTTATATGGTGATTAGAATAGGAGAGAGATGTCCCGAGTATGGAAATCACATGGATCCCGAAGCCCATATTGAAACATGTTGTGGTATTCTATGGCGGGAGCTAGAGCAATCTGGAGATGAAGTTTTGCTGTTGCTTATGCAATGTGC
TGAGGAGCTTCCACACAAGAGTCCCTTCTATGGAACACTGGTGGGCTTGTTGAATCTAGAAGATGAAGAGCTTGGTAGGAAGGTTGTAGAACAAACTCATGCCAGACTGCAGGTTTGTTTTCAGTCTTTTCCAATATTTAGTGTCATTTCTAAAATTGGCATACTTTCTCAATTGTGCAGCGA

>TRINITY_DN105617_c0_g1_i3 from sample A5 / Example unspliced isoform:

CGACTGAACTATTCACAGGCAGAGCATTACCGAGTCTGTGAGAAACGAAGGCGTTGCAGGTGAAGCTGAAGCCTTGTAGAAGCCATTGCACAGCATACATTTTTATTAAATgtaagtatggggatgccaattgagcattttgttctgtgtgtgtgttttgcgaaggttttcatggatgattgaagcatgtatctatttac
ctgcagAGGAATAAGAAGAAACCATGAATAGCTGGCGTTATATGGTGATTAGAATAGGAGAGAGATGTCCCGAGTATGGAAATCACATGGATCCCGAAGCCCATATTGTGAGCATTTCTCTTCATCTTTATTTTTGTTTGTTTGTCATGTGTATTTATTGCTCTAAGTAACACCC

Intron 2 ATTTTTTGGA ... TGTCCTGAAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.73	21	37	75
A2	74.24	17	39	59
A3	81.67	11	40	58
A4	78.82	9	28	39
A5	72.41	8	13	29
A6	62.5	15	23	27
A7	72.06	19	33	65
A8	50.88	14	13	16
A9	77.19	13	36	52
A10	83.21	11	44	65

>TRINITY_DN119821_c0_g1_i12 from sample A2 / Example spliced isoform

AGTAACGGGAAAAAGTTATGATTCTGGACCACTGACATGTCCTGAACAGCCTGCGCTACCTTCAACGATTTCTGATGTAACAGCTGGTAGACAGAGACACGAGGCTGAATTGAAATATCCAAGGAGAATTCAAAGGTTGCGGTTATTTCCACAGTCAAAAACAGAGGAGGAGTTGCAGCCAATAGATCGTTTTGTGGTTG
AGGAGTATTTGCTTGATGTGCTGCTATACATGAATGGCTGGAGCCTGCGCTACCTTCAACGATTTCTGATGTAACAGCTGGTAGACAGAGACACGAGGCTGAATTGAAATATCCAAGGAGAATTCAAAGGTTGCGGTTATTTCCACAGTCAAAAACAGAGGAGGAGTTGCAGCCAATAGATCGTTTTGTGGTTGAGGAGT
ATTTGCTTGATGTGCTGCTATACATGAATGGCTGCCGGAAGGAATTTCCTGTATACATGGTTGGCTTACCAGTCTCATTTCGGTATGAATATCTTATGGCAGAAACAATATTCTCTCAGGTAATGCTTTTGGAAAGAAGGTAGCTGTA

>TRINITY_DN119821_c0_g1_i9 from sample A2 / Example unspliced isoform:

AGTAACGGGAAAAAGTTATGATTCTGGACCACTGACATGTCCTGAACAGCCTGCGCTACCTTCAACGATTTCTGATGTAACAGCTGGTAGACAGAGACACGAGGCTGAATTGAAATATCCAAGGAGAATTCAAAGGTTGCGGTTATTTCCACAGTCAAAAACAGAGGAGGAGTTGCAGCCAATAGATCGTTTTGTGGTTG
AGGAGTATTTGCTTGATGTGCTGCTATACATGAATGGCTGGattttttggaagatttatggaggcgcatacaatcactatctgacaatggatggaaaattgatagtgttccacgaccacatcttgcatttgaggcccggttagtaacgggaaaaagttatgattttggaccactgacatgtcctgaacAGCCTGCGCTAC
CTTCAACGATTTCTGATGTAACAGCTGGTAGACAGAGACACGAGGCTGAATTGAAATATCCAAGGAGAATTCAAAGGTTGCGGTTATTTCCACAGTCAAAAACAGAGGAGGAGTTGCAGCCAATAGATCGTTTTGTGGTTGAGGAGTATTTGCTTGATGTGCTGCTATACATGAATGGCTGCCGGAAGGAATTTCCTGTA
TACATGGTTGGCTTACCAGTCTCATTTCGGTATGAATATCTTATGGCAGAAACAATATTCTCTCAG

Intron 3 GTAAACAATT ... TAAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.77	69	0	17
A2	20.25	63	2	16
A3	22.92	37	0	11
A4	13.85	56	0	9
A5	3.57	27	0	1
A6	19.01	230	1	54
A7	24.16	226	0	72
A8	21.33	284	0	77
A9	24.97	231.3	0	77
A10	16.32	159	0	31

>TRINITY_DN119821_c0_g1_i1 from sample A2 / Example spliced isoform

TTAAAGAGGTTGAAGAATACCTATGCTGTCTGCATGGTTTTCCAGTTACAAATTACAAGCAAGTCCAAAACTGTAAGAGATTGTTTAAGGTGCCAACTGCCATGTTTTCTCTGTTATTCTAGCAGGTCCTGTGCATCTTTTATTTCCCAGTTAGCTGTGATAATGACTAAATTAATTCATGCTATTCAATTTGAATGCAG
GCATTGCTCTTTGCTCTTTATAAAAACTTTTTGGACGTGCTTATGAAACATCTCTCGGATGATCGTATTGAGAAACCAATTGATGGTCCTGAACCTGATGAAAGAGATTCCATGGCTGTAGATAAGGAACCTTCAGCAATGGATGCTGATGATGATGATGAACAGAGGAATCAAAGGTTTACCAAGAGTAATGGATTGCA
TACAGAAGAAGAACAGGAGTGGTGTCGGGTCACTCTGGGCCAGGTTCGGGCATTGACAAGGCAGTATGCAACAGAGATTTGGCCTCATATAGAGAAGTTAGATGCTGAAGTTTTCACTGGGGATGTGCATCCTTCTATTGTAAAAGCAGTATATGATGGCCTAAGACGTCCATTTTCTCAATTTGAAGGGGACAGTATGA
TGGTGAAAATTTAATCCCTTATATGGTTGATGGGAAAAATTTAAGCCCTTGTATGGCAGCCGTGAAGTAAATGTTTTTGCTACTGAATTTGATCTATGTTTAATTGCGTTGTTTAGTGGAATTTGGTTTAAGAGGCGGGCATCTCAATGGCTTCTTTTGCAATTTTTGGGTTAATGAATTCCTTTGATTTCTGGGGAAGG
TAATGAGAGGTGTACCATATTACAGTCATGCGGAAGTTTTCTCCCTTGTTAAGCCATTGTAGTCAAATAGAGTAAAATATCTCAGGGAAGACCTGGTTTTAGGGACTTGGAAATGTTCTCTATCCAATATTCTCTATCAGGGTCACAATCAATAGTTTGTCCTGAATACGTTCTAGCACAGAACGTGCTAGATGCTAATC
TGATCAAACTTGCATGTAACTTTATCTGACAATCTTATGTTGGTTTCCAGTTTCTTAATTGTAGAATGTGATTTCTGTGGCATTGTATAGTGCATTTATGCATTTCTAAATTTTATAATTTTTTCA

>TRINITY_DN119821_c0_g1_i15 from sample A2 / Example unspliced isoform:

TGACAAGGCAGTATGCAACAGAGgtaaacaattcagctgattgtcaaattatgcagatttgagctaagtaatggtatactgtggtgtctccttgctgatttagtctcttaaattgcagATTTGGCCTCATATAGAGAAGTTAGATGCTGAAGTTTTCACTGGGGATGTGCATCCTTCTATTGTAAAAGCAGTATATGATG
GCCTAAGACGTCCATTTTCTCAATTTGAAGGGGACAGTATGATGGTGAAAATTTAATCCCTTATATGGTTGATGGGAAAAATTTAAGCCCTTGTATGGCAGCCGTGAAGTAAATGTTTTTGCTACTGAATTTGATCTATGTTTAATTGCGTTGTTTAGTGGAATTTGGTTTAAGAGGCGGGCATCTCAATGGCTTCTTTT
GCAATTTTTGGGTTAATGAATTCCTTTGATTTCTGGGGAAGGTAATGAGAGGTGTACCATATTACAGTCATGCGGAAGTTTTCTCCCTTGTTAAGCCATTGTAGTCAAATAGAGTAAAATATCTCAGGGAAGACCTGGTTTTAGGGACTTGGAAATGTTCTCTATCCAATATTCTCTATCAGGGTCACAATCAATAGTTT
GTCCTGAATACGTTCTAGCACAGAACGTGCTAGATGCTAATCTGATCAAACTTGCATGTAACTTTATCTGACAATCTTATGTTGGTTTCCAGTTTCTTAATTGTAGAATGTGATTTCTGTGGCATTGTATAGTGCATTTATGCATTTCTAAATTTTATAATTTTTTCA