NA ----> NA

Intron 1 GCAATATTTC ... CTTTGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.3	12.7	3	8
A2	17.19	11.6	0.8	4
A3	35.24	4	2.4	2
A4	10	9	2	0
A5	25.88	6	2.2	2
A6	12.12	18.2	3	2
A7	18.28	17	2.6	5
A8	25.06	21.2	4	10
A9	32.41	18.1	5	12
A10	26.67	27	11.6	8

>TRINITY_DN45453_c5_g1_i8 from sample A7 / Example spliced isoform

GGAAATATAGCAATCCTCACCCGTGTGGGACATATATCACAACCGCTACTTCTTCTGCCTTGGGCGCGTCACGTTCCAATCCAATTGCCAATTCCACAACTCTTGTCCTATTCAGCCAGCCAGCCAGTTCGTGCGTCAATATACATTTGACTGGAAGATGAAGAAGAAATTATACACGTTTTTTTTTTCCATTTCTTACT
GCGCAATTGATGATCATTTTCTTCTTGTTGATGAAGGAGAGATGCAGAGTGATCTGAGTCGAGAGCCTGGGCGATGTCACTGGTGTGCGGAATCCCCCTGCTGGAATGCGTGTATTGCCTAGCCTGTGCTCGGTGGGCGTGGAAGCGGTGCCTGCACACGGGCGAGCAAGACAGCGAGCGATGGGGCCATGCGAGTCGGG
AGGAGTTCGAACCCGTGCCCCGACTCTGCCGCCTCATCCTCTCCGTATACGAAGACGACCTAAGGAACCCCCAGTGGGCTCCTCCGGGAGGTTACGGGATCAATCCCGAGTGGATCCTCAAGAAAGTCACCTACGAGCAGACTTTGGGTCGAGCTCCTCCTTATGTTATCTACTTGGATCATGAAAATAGCGACATTGTG
CTGGCAATACGAGGCCTGAACATGGCAATGGAGAGCGATTATGCCTTGCTCTGGGACAACAAGCTCGGGCAGAAGATGTTCGACGGGGGTTTTGTTCATAACGGGCTCTTGAAATCCGCTGCCTGGCTTCTCAACAACGAGTCCGATACGATTAAAGAATTGGTTGGGGAGTACCCGGATTACACTCTCACTTTTACTGG
GCACTCGCTTGGGTCCGGGATAGCGGCTCTGATGGCTTTGATCGTGGCCAATAACAGGGACCTGTTGGATGAAATACCTAGGAATCAGATTAGATGTTATGCGTTTGCTCCTGCCAGATGCATGTCTCTCAACTTGGCTGTCAGATATGCCGACATTGTCAATTCTGTTGTGTTACAGCTAATTGTTCCAGGATGACTTC
TTGCCAAGGACAGCTACTCCCCTGGAAGATATTTTTGAATCAGTTTTTTGCTTGCCTTGTTTACTCTGTGGCAGATGCATGAGTGATACATGTATACCAGAAGAAAGGATGCTTGCAGATCCACGAAGATTATATGCTCCAGGACGGTTGTACCACATTGTGGAAAGAAAACTTTGCAGATGTGGAAGGTTTCCACCAGT
TGTGAAAACTGCTGTTCCAGTAGAGGGAAGATTTGAGCACATTATCATTTCATGCAATGCTACATCAGACCATGCAATTATTTGGATTGAGAGGGAGTCACAAAAAGCGCTAGATATCATATGTCCCTGGCTGGGAGTTTTGGTCCCCAAAAGCTTTTTGGTGGTAGGAGATGGAAGCACGTGGCCTCTTTCTCTCCAAG
TCCG

>TRINITY_DN45453_c5_g1_i7 from sample A7 / Example unspliced isoform:

GGAAATATAGCAATCCTCACCCGTGTGGGACATATATCACAACCGCTACTTCTTCTGCCTTGGGCGCGTCACGTTCCAATCCAATTGCCAATTCCACAACTCTTGTCCTATTCAGCCAGCCAGCCAGTTCGTGCGTCAATATACATTTGACTGGAAGATGAAGAAGAAATTATACACGTTTTTTTTTTCCATTTCTTACT
GCGCAATTGATGATCATTTTCTTCTTGTTGATGAAGGAGAGATGCAGAGTGATCTGAGTCGAGAGCCTGGGCGATGTCACTGGTGTGCGGAATCCCCCTGCTGGAATGCGTGTATTGCCTAGCCTGTGCTCGGTGGGCGTGGAAGCGGTGCCTGCACACGGGCGAGCAAGACAGCGAGCGATGGGGCCATGCGAGTCGGG
AGGAGTTCGAACCCGTGCCCCGACTCTGCCGCCTCATCCTCTCCGTATACGAAGACGACCTAAGGAACCCCCAGTGGGCTCCTCCGGGAGGTTACGGGATCAATCCCGAGTGGATCCTCAAGAAAGTCACCTACGAGCAGACTTTGGGTCGAGCTCCTCCTTATGTTATCTACTTGGATCATGAAAATAGCGACATTGTG
CTGGCAATACGAGGCCTGAACATGGCAATGGAGAGCGATTATGCCTTGCTCTGGGACAACAAGCTCGGGCAGAAGATGTTCGACGGGGGTTTTGTTCATAACGGGCTCTTGAAATCCGCTGCCTGGCTTCTCAACAACGAGTCCGATACGATTAAAGAATTGGTTGGGGAGTACCCGGATTACACTCTCACTTTTACTGG
GCACTCGCTTGGGTCCGGGATAGCGGCTCTGATGGCTTTGATCGTGGCCAATAACAGGGACCTGTTGGATGAAATACCTAGGAATCAGATTAGATGTTATGCGTTTGCTCCTGCCAGATGCATGTCTCTCAACTTGGCTGTCAGATATGCCGACATTGTCAATTCTGTTGTGTTACAGCTAATTGTTCCAGGATGACTTC
TTGCCAAGGACAGCTACTCCCCTGGAAGATATTTTTGAATCAGTTTTTTGgcaatatttcaaacctgtggcatagatctctctttattggatattaaaggtgtgtatgcctttggacagCTTGCCTTGTTTACTCTGTGGCAGATGCATGAGTGATACATGTATACCAGAAGAAAGGATGCTTGCAGATCCACGAAGATT
ATATGCTCCAGGACGGTTGTACCACATTGTGGAAAGAAAACTTTGCAGATGTGGAAGGTTTCCACCAGTTGTGAAAACTGCTGTTCCAGTAGAGGGAAGATTTGAGCACATTATCATTTCATGCAATGCTACATCAGACCATGCAATTATTTGGATTGAGAGGGAGTCACAAAAAGCGCTAGATCTAATGATTGAAAGGG
AAAGAGCCATGGAGATACCTTCTGAACAAAAAATGCGCAGACAACAGACACTTGCAAATGAACATGATAAAGAGTACAAGGCTGCTATCGAGAAGGCTGCTACACTGAGAATGGCCCAGGCACACTCTCCATCTTATGGGACTTTTGATACTGTCTCTGATAACAATGACAATGAAGAAACTGATCTTTTGGATGAACAT
GGGGAAAGTTTCTCCAAGAAACCTGAAATTGGGGAGCCTTCAATAACAGATAAAAGAAAGTTCAAATGGGATGAAGTGATCAATAAACTTATAAAGAAAAAAGAAGACTCGTGAAAGTTAAAGAAAGGGGATGGATAGTCTCTCTAATGATTCTCGATTTCTACTTTCTGCAAGTTAAATGCAGGT

Intron 2 GTATGAATAG ... CTTTGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.48	13	12	8
A2	13.33	13	0	4
A3	20	4	0	2
A4	14.29	9	3	0
A5	14.29	6	1	1
A6	5	19	0	2
A7	17.07	17	1	6
A8	15.38	22	0	8
A9	20.83	19	0	10
A10	8.2	28	0	5

>TRINITY_DN107579_c0_g1_i1 from sample A1 / Example spliced isoform

CCGCTACTTCTTCTGCCTTGGGCGCGTCACGTTCCAATCCAATTGCCAATTCCACAACTCTTGTCCTATTCAGCCAGCCAGCCAGTTCGTGCGTCAATATACATTTGACTGGAAGATGAAGAAGAAATTATACACGTTTTTTTTTTCCATTTCTTACTGCGCAATTGATGATCATTTTCTTCTTGTTGATGAAGGAGAGA
TGCAGAGTGATCTGAGTCGAGAGCCTGGGCGATGTCACTGGTGTGCGGAATCCCCCTGCTGGAATGCGTGTATTGCCTAGCCTGTGCTCGGTGGGCGTGGAAGCGGTGCCTGCACACGGGCGAGCAAGACAGCGAGCGATGGGGCCATGCGAGTCGGGAGGAGTTCGAACCCGTGCCCCGACTCTGCCGCCTCATCCTCT
CCGTATACGAAGACGACCTAAGGAACCCCCAGTGGGCTCCTCCGGGAGGTTACGGGATCAATCCCGAGTGGATCCTCAAGAAAGTCACCTACGAGCAGACTTTGGGTCGAGCTCCTCCTTATGTTATCTACTTGGATCATGAAAATAGCGACATTGTGCTGGCAATACGAGGCCTGAACATGGCAATGGAGAGCGATTAT
GCCTTGCTCTGGGACAACAAGCTCGGGCAGAAGATGTTCGACGGGGGTTTTGTTCATAACGGGCTCTTGAAATCCGCTGCCTGGCTTCTCAACAACGAGTCCGATACGATTAAAGAATTGGTTGGGGAGTACCCGGATTACACTCTCACTTTTACTGGGCACTCGCTTGGGTCCGGGATAGCGGCTCTGATGGCTTTGAT
CGTGGCCAATAACAGGGACCTGTTGGATGAAATACCTAGGAATCAGATTAGATGTTATGCGTTTGCTCCTGCCAGATGCATGTCTCTCAACTTGGCTGTCAGATATGCCGACATTGTCAATTCTGTTGTGTTACAGGATGACTTCTTGCCAAGGACAGCTACTCCCCTGGAAGATATTTTTGAATCAGTTTTTTGCTTGC
CTTGTTTACTCTGTGGCAGATGCATGAGTGATACATGTATACCAGAAGAAAGGATGCTTGCAGATCCACGAAGATTATATGCTCCAGGACGGTTGTACCACATTGTGGAAAGAAAACTTTGCAGATGTGGAAGGTTTCCACCAGTTGTGAAAACTGCTGTTCCAGTAGAGGGAAGATTTGAGCACATTATCATTTCATGC
AATGCTACATCAGACCATGCAATTATTTGGATTGAGAGGGAGTCACAAAAAGCGCTAGATCTAATGATTGAAAGGGAAAGAGCCATGGAGATACCTTCTGAACAAAAAATGCGCAGACAACAGACACTTGCAAATGAACATGATAAAGAGTACAAGGCTGCTATCGAGAAGGCTGCTACACTGAGAATGGCCCAGGCACA
CTCTCCATCTTATGGGACTTTTGATACTGTCTCTGATAACAATGACAATGAAGAAACTGATCTTTTGGATGAACATGGGGAAAGTTTCTCCAAGAAACCTGAAATTGGGGAGCCTTCAATAACAGATAAAAGAAAGTTCAAATGGGATGAAGTGATCAATAAACTTATAAAGAAAAAAGAAGACTCGTGAAAGTTAAAGA
AAGGGGATGGATAGTCTCTCTAATGATTCTCGATTTCTACTTTCTGCAAGTTAAATGCAGGTTTGGTTCTTCAATTTATTGTTTTTTCATTCCCTAAATTGTATCGTGTATTATAATTTATGGATACACAAGTAGGTGTGCAGGTTGTTTTGTTGATTTCTTTATTAGTTATACATCCAATTTTGGAGCTGACATAAGAA
GTTGAAACTAAAATACACAGATTGATTCAGAGATAAGTTTCACACCCCATGCCTACATTTTGTTTGCATTTGGATTTATATATACAAGCTTGATCATTCAAGTCAAATAGAAATTTCCATTAGAATGGTTTTCCTCCTTGTTTGTCAGATAAATCCCTGTGCACACTGCCTGCCTCAATGTAGACCTGGCGAGTCAAACT
CACCGGTCTGTCCTGAAAAACTCTCCTCTTAAGTACATGTTATCACACAACTTGTAGTTTCCCCTATCAGATGATCCTATCAAGGACGGTAGAGGAGCTAGGCAACTAAAGAGGCGACCTAAGGGGGCCTTGATCCCTGGATAGATCGGTTGTCATGCCAATCG

>TRINITY_DN107579_c0_g1_i2 from sample A1 / Example unspliced isoform:

GGCACGTGTAATGCCAAATCTGAATTATATGCTCTCTTGTCTGGGAGTCGCACCCTACAATTGAATCTTCCTGATAGACACTGAATGACAGTCTCAGTTTTAACTCTTTAAGTTCTTGTAGCAATTTACTTGAAATCTTTTGTATTTTAATAGTAGTAAGCTAGTGACCAGTTTACATTCCTGATATTCAGTAGTCTGAA
TGGTATATAAAGTCTATGCTTTATCAACTTTTTTATATTTATGATTCTAAGCATAACATGGTTTGTACTTACTGTACTCTTATTCAAGCTAATTGTTCCAGGATGACTTCTTGCCAAGGACAGCTACTCCCCTGGAAGATATTTTTGAATCAGTTTTTTGgtatgaatagtttctctctttggacattttatgatatgcc
atcatatttgatttcactttttcaatccttgtcatctttatttttaatatcctattaggtatgtcttattgattaatgaattgtactcatcatatcacattacaaccttaagaacttttggtgttattctttctataatcagtagccatggtaaaatattctaatctcctcattttattaggcaatatttcaaacctgtg
gcatagatctctctttattggatattaaaggtgtgtatgcctttggacagCTTGCCTTGTTTACTCTGTGGCAGATGCATGAGTGATACATGTATACCAGAAGAAAGGATGCTTGCAGATCCACGAAGATTATATGCTCCAGGACGGTTGTACCACATTGTGGAAAGAAAACTTTGCAGATGTGGAAGGTTTCCACCAGT
TGTGAAAACTGCTGTTCCAGTAGAGGGAAGATTTGAGCACATTATCATTTCATGCAATGCTACATCAGACCATGCAATTATTTGGATTGAGAGGGAGTCACAAAAAGCGCTAGATCTAATGATTGAAAGGGAAAGAGCCATGGAGATACCTTCTGAACAAAAAATGCGCAGACAACAGACACTTGCAAATGAACATGATA
AAGAGTACAAGGCTGCTATCGAGAAGGCTGCTACACTGAGAATGGCCCAGGCACACTCTCCATCTTATGGGACTTTTGATACTGTCTCTGATAACAATGACAATGAAGAAACTGATCTTTTGGATGAACATGGGGAAAGTTTCTCCAAGAAACCTGAAATTGGGGAGCCTTCAATAACAGATAAAAGAAAGTTCAAATGG
GATGAAGTGATCAATAAACTTATAAAGAAAAAAGAAGACTCGTGAAAGTTAAAGAAAGGGGATGGATAGTCTCTCTAATGATTCTCGATTTCTACTTTCTGCAAGTTAAATGCAGGTTTGGTTCTTCAATTTATTGTTTTTTCATTCCCTAAATTGTATCGTGTATTATAATTTATGGATACACAAGTAGGTGTGCAGGT
TGTTTTGTTGATTTCTTTATTAGTTATACATCCAATTTTGGAGCTGACATAAGAAGTTGAAACTAAAATACACAGATTGATTCAGAGATAAGTTTCACACCCCATGCCTACATTTTGTTTGCATTTGGATTTATATATACAAGCTTGATCATTCAAGTCAAATAGAAATTTCCATTAGAATGGTTTTCCTCCTTGTTTGT
CAGATAAATCCCTGTGCACACTGCCTGCCTCAATGTAGACCTGGCGAGTCAAACTCACCGGTCTGTCCTGAAAAACTCTCCTCTTAAGTACATGTTATCACACAACTTGTAGTTTCCCCTATCAGATGATCCTATCAAGGACGGTAGAGGAGCTAGGCAACTAAAGAGGCGACCTAAGGGGGCCTTGATCCCTGGATAGA
TCGGTTGTCATGCCAATCG