sp|Q9LF79|ACA8_ARATH ----> Calcium-transporting ATPase 8, plasma membrane-type

Intron 1 GTATGTACCT ... TTTCCTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.56	408	4	64
A2	18.62	306	2	70
A3	15.99	310	1	59
A4	15.58	260	2	48
A5	6.42	248	0	17
A6	10.87	82	0	10
A7	9.73	269	0	29
A8	15.04	113	0	20
A9	9.79	212	0	23
A10	12.2	324	0	45

>TRINITY_DN124992_c1_g1_i16 from sample A1 / Example spliced isoform

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTATTAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTTTTTGTGT
GGGCTGCTCTTCAGGACACGACACTCGTCATTTTGATGATTTGTGCGGTCTTGTCACTTGCTTTGGGCATAAAAACGGAAGGCATTAAGGAAGGGTGGTTAGATGGTGCAAGTATATCTTTTGCTGTGATCCTTGTCATTCTTGTTACAGCAATCAGTGATTACAGACAGTCTCTCCAGTTCCAAAATCTCAATGAAGAG
AAGGAAAACATTCAACTTCAGGTGATCAGAGGTGGAAGAATATTTAAAATTTCTATATATGATCTTGTTGTTGGTGATGTTGTCCCCCTCAATATTGGTGATCAGGTGCCAGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGACTGGGGAGAGCAAGTTTGTTTATAAAGATCAGAA
AAATCCCTTCATGATGTCTGGCTGTAAGGTGGCAGATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTGGTAAAGTTGGACTTGCAGTGGCAGTTGTAA
CCTTCGTTATCCTTTTGATTAGATTCTTTACTGGACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

>TRINITY_DN124992_c1_g1_i2 from sample A1 / Example unspliced isoform:

CTGGGGAGAGCAAGTTTgtatgtaccttttttgctgatatcttagtttcttggcctgttagcaaagcagtgaattttcccagaagcttgtgatgctaaagtttgtaacttttgcatcaggctatgaacgcctctttgtttttcctctagGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCA
GATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTGGTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGG
ACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

Intron 2 GTCTGTCTTC ... TTTATAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	1.78	193	3	4
A2	5.29	152.3	3	14
A3	1.1	180.3	0	4
A4	4.69	152.3	11	4
A5	3.58	80.7	3	3
A6	2.83	34.3	0	2
A7	3.5	110.3	0	8
A8	1.08	45.7	0	1
A9	1.51	97.7	0	3
A10	1.07	139.3	0	3

>TRINITY_DN124992_c1_g1_i2 from sample A1 / Example spliced isoform

CTGGGGAGAGCAAGTTTGTATGTACCTTTTTTGCTGATATCTTAGTTTCTTGGCCTGTTAGCAAAGCAGTGAATTTTCCCAGAAGCTTGTGATGCTAAAGTTTGTAACTTTTGCATCAGGCTATGAACGCCTCTTTGTTTTTCCTCTAGGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCA
GATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTGGTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGG
ACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

>TRINITY_DN124992_c1_g1_i5 from sample A1 / Example unspliced isoform:

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTATTAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTTTTTGTGT
GGGCTGCTCTTCAGGACACGACACTCGTCATTTTGATGATTTGTGCGGTCTTGTCACTTGCTTTGGGCATAAAAACGGAAGGCATTAAGGAAGGGTGGTTAGATGGTGCAAGTATATCTTTTGCTGTGATCCTTGTCATTCTTGTTACAGCAATCAGTGATTACAGACAGTCTCTCCAGTTCCAAAATCTCAATGAAGAG
AAGGAAAACATTCAACTTCAGGTGATCAGAGGTGGAAGAATATTTAAAATTTCTATATATGATCTTGTTGTTGGTGATGTTGTCCCCCTCAATATTGGTGATCAGGTGCCAGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGACTGGGGAGAGCAAGTTTGCTATGAACGCCTCTTT
GTTTTTCCTCTAGGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCAGATGGCTATGGTACAATGTTGGTGAGTTGTTATGCCTGGTTATTTGTCTGGAAATAATATCGCCACTTATGATTTGAGCAGCCCCGTTGACCTACTTTTGGTTATTTGTTGGACATCAACAGACTTATCTTATCTT
TCTCTATCATTGGTGTTTTTTGCCATGTTTGATAATAGCGTGGGGTGGGTTGGATTTTTATTACCTTTACAGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGgtctgtcttccctgccttgtttaatttgtatgctattctttgac
ttgagtggtagcttcagaatctcagatatttcagtcacaaagactctcccgtttataatagGTCCGCCTCAATGGTGTTGCAACTTTAATTGGTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGGACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCA
AGACCAAGATTGGAGATGCC

Intron 3 GTATGTACCT ... TTTGCATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.92	37	4	11
A2	27.27	24	2	9
A3	18.75	26	1	6
A4	28.57	20	2	8
A5	4.55	21	0	1
A6	11.11	8	0	1
A7	4.55	21	0	1
A8	22.22	14	0	4
A9	5.56	34	0	2
A10	5.26	18	0	1

>TRINITY_DN124992_c1_g1_i6 from sample A1 / Example spliced isoform

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTATTAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTTTTTGTGT
GGGCTGCTCTTCAGGACACGACACTCGTCATTTTGATGATTTGTGCGGTCTTGTCACTTGCTTTGGGCATAAAAACGGAAGGCATTAAGGAAGGGTGGTTAGATGGTGCAAGTATATCTTTTGCTGTGATCCTTGTCATTCTTGTTACAGCAATCAGTGATTACAGACAGTCTCTCCAGTTCCAAAATCTCAATGAAGAG
AAGGAAAACATTCAACTTCAGGTGATCAGAGGTGGAAGAATATTTAAAATTTCTATATATGATCTTGTTGTTGGTGATGTTGTCCCCCTCAATATTGGTGATCAGGTGCCAGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGACTGGGGAGAGCAAGTTTGCTATGAACGCCTCTTT
GTTTTTCCTCTAGGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCAGATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTG
GTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGGACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

>TRINITY_DN124992_c1_g1_i2 from sample A1 / Example unspliced isoform:

CTGGGGAGAGCAAGTTTgtatgtaccttttttgctgatatcttagtttcttggcctgttagcaaagcagtgaattttcccagaagcttgtgatgctaaagtttgtaacttttgcatcagGCTATGAACGCCTCTTTGTTTTTCCTCTAGGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCA
GATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTGGTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGG
ACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

Intron 4 GTAAGATTGA ... ACGCTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	13.66	72.7	11	12
A2	18.6	35	7	9
A3	19.59	51.3	11	14
A4	19.37	33.3	4	12
A5	14.29	30	0	10
A6	0	1	0	0
A7	9.93	22.7	0	5
A8	12.82	17	0	5
A9	22.22	7	2	2
A10	13.33	13	0	4

>TRINITY_DN124992_c1_g1_i12 from sample A1 / Example spliced isoform

CGGCATTTGAAGTCTAAAGCATTCCTAAATCCCTACCTTTTTTTCTTCTTTCAGGCAGCCTTTATCTTCAAGAGAGCTGCTATAAAGCCAAATGAAGAGGAGGAAGCACCCCCAGAAGGTTTTGGAATTGGACGAACAACAGTTACGAGGTTGGTTAGAGAACATAATGTTAATGCTCTGCAGCAGATGGGCGGGGTAAA
TCAAGTAGCCAATCTATTACATACTGATCTTGACAAGGGTATCAAGGGAAGTCCAGAAGAAATTCAAAGTCGAAGGAATGCATATGGAGAGAATTCGTATCCACCAAAGGCTGTACAGAATTTTTTTGATTTTGTGTGGGAAGCTCTTCAGGACATGATACTCATCATTTTGATGATTTGCGCGGCCTTGTCACTTGCTT
TAGGCATAAAAACTGAGGACATTAAGGAAGGGTGGTTTAATGGCGGAAGTATAGGTTTTGCTGTGATCCTTGTCATTCTTGT

>TRINITY_DN124992_c1_g1_i10 from sample A1 / Example unspliced isoform:

AACATTGAACTGCATATTAAATATCATTGCAGAATGTAATTTAATGGTTATTGGCAAGAATCATTAAAATTAGTTATCTTGACATTAACATTACTTTTCTAATCAATCCTTCATCATGCACATATGCAGAAAGCAACATTGGTTCTAAATGCAACCCGCCGGTTTAGGTATACTTTAGATCTAAAAAGGGAAAAGGAGAT
AGAACAGATTAAAAGCAAGCTTAGAGCCCATGCTCAGTCTATAAGGGCAGCCTTTATCTTCAAGAGAGCTGCTATAAAGCCAAATGAAGAGGAGGAAGCACCCCCAGAAGGTTTTGGAATTGGACGAACAACAGTTACGAGGTTGGTTAGAGAACATAATGTTAATGCTCTGCAGCAGATGGGCGGGgtaagattgaaaa
gacatttcagattttgattttgtttgcacatttcaattaattgatcactttatactttatgttcctgctgaggtagttattggagtgacaacctggaacgctaacagGTAAATCAAGTAGCCAATCTATTACATACTGATCTTGACAAGGGTATCAAGGGAAGTCCAGAAGAAATTCAAAGTCGAAGGAATGCATATGGA
GAGAATTCGTATCCACCAAAGGCTGATTTTGTGTGGGAAGCTCTTCAGGACATGATACTCATCATTTTGATGATTTGCGCGGCCTTGTCACTTGCTTTAGGCATAAAAACTGAGGACATTAAGGAAGGGTGGTTTAATGGCGGAAGTATAGGTTTTGCTGTGATCCTTGTCATTCTTGTCACAGCAATAAGTGATTACAG
ACAATCTCTCCAGTTCCAAAATCTGAATGAAGAGAAGAAAAACATTCAACTTGAGGTGATCAGAGGTGGAAGAAGATGTAAAGTTTCCATATTTGATCTTGTTGTTGGTGATGTTGTACCCCTCAATATTGGTGATCAAGTCCCTGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGA
CTGGGGAGAGCAAGTTTG

Intron 5 GTAATGTGCT ... CCTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.14	44	14	2
A2	25	18	6	0
A3	27.27	24	9	0
A4	25.93	20	7	1
A5	18.92	30	7	0
A6	0	2	0	0
A7	13.64	19	3	0
A8	28.57	5	2	0
A9	50	4	4	0
A10	21.74	18	5	0

>TRINITY_DN124992_c1_g1_i6 from sample A1 / Example spliced isoform

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTATTAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTTTTTGTGT
GGGCTGCTCTTCAGGACACGACACTCGTCATTTTGATGATTTGTGCGGTCTTGTCACTTGCTTTGGGCATAAAAACGGAAGGCATTAAGGAAGGGTGGTTAGATGGTGCAAGTATATCTTTTGCTGTGATCCTTGTCATTCTTGTTACAGCAATCAGTGATTACAGACAGTCTCTCCAGTTCCAAAATCTCAATGAAGAG
AAGGAAAACATTCAACTTCAGGTGATCAGAGGTGGAAGAATATTTAAAATTTCTATATATGATCTTGTTGTTGGTGATGTTGTCCCCCTCAATATTGGTGATCAGGTGCCAGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGACTGGGGAGAGCAAGTTTGCTATGAACGCCTCTTT
GTTTTTCCTCTAGGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCAGATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTG
GTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGGACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

>TRINITY_DN124992_c1_g1_i4 from sample A1 / Example unspliced isoform:

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAGCTGAGAAAAAGATTGGGATTTTGATTTTGTTCATACATTTGAATTACCTGTTGAGGTAGTTATTACAGTGACAACCTGAAATGCTAACAGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTAT
TAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTgtaatgtgctctttagcattcatgatagatcaaagtgtatatttgctctacagaagtatttatgatcctgtctgaaaatggcagtgtagtaatttgcatatctgaacttatatcc
tttttcagGTTTTTGTGTGGGC

Intron 6 GCTATGAACG ... TTTCCTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	7.9	408	35	35
A2	7.27	306	24	32.5
A3	7.74	310	26	38.5
A4	7.14	260	20	25
A5	7.81	248	21	11
A6	8.38	82	7.5	7
A7	7.08	269	20.5	16
A8	10.31	113	13	11
A9	13.65	212	33.5	19
A10	5.26	324	18	23

>TRINITY_DN124992_c1_g1_i16 from sample A1 / Example spliced isoform

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTATTAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTTTTTGTGT
GGGCTGCTCTTCAGGACACGACACTCGTCATTTTGATGATTTGTGCGGTCTTGTCACTTGCTTTGGGCATAAAAACGGAAGGCATTAAGGAAGGGTGGTTAGATGGTGCAAGTATATCTTTTGCTGTGATCCTTGTCATTCTTGTTACAGCAATCAGTGATTACAGACAGTCTCTCCAGTTCCAAAATCTCAATGAAGAG
AAGGAAAACATTCAACTTCAGGTGATCAGAGGTGGAAGAATATTTAAAATTTCTATATATGATCTTGTTGTTGGTGATGTTGTCCCCCTCAATATTGGTGATCAGGTGCCAGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGACTGGGGAGAGCAAGTTTGTTTATAAAGATCAGAA
AAATCCCTTCATGATGTCTGGCTGTAAGGTGGCAGATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTGGTAAAGTTGGACTTGCAGTGGCAGTTGTAA
CCTTCGTTATCCTTTTGATTAGATTCTTTACTGGACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

>TRINITY_DN124992_c1_g1_i6 from sample A1 / Example unspliced isoform:

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTATTAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTTTTTGTGT
GGGCTGCTCTTCAGGACACGACACTCGTCATTTTGATGATTTGTGCGGTCTTGTCACTTGCTTTGGGCATAAAAACGGAAGGCATTAAGGAAGGGTGGTTAGATGGTGCAAGTATATCTTTTGCTGTGATCCTTGTCATTCTTGTTACAGCAATCAGTGATTACAGACAGTCTCTCCAGTTCCAAAATCTCAATGAAGAG
AAGGAAAACATTCAACTTCAGGTGATCAGAGGTGGAAGAATATTTAAAATTTCTATATATGATCTTGTTGTTGGTGATGTTGTCCCCCTCAATATTGGTGATCAGGTGCCAGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGACTGGGGAGAGCAAGTTTgctatgaacgcctcttt
gtttttcctctagGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCAGATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTG
GTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGGACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

Intron 7 GTGAGTTGTT ... ACCTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	2.37	209	6	3
A2	2.23	187	4	4
A3	3.08	191.7	8	3
A4	4.98	156	4	11
A5	1.17	105.3	2	0
A6	1.28	42.3	1	0
A7	0.51	113.3	1	0
A8	2.01	53	2	0
A9	1.88	89.7	3	0
A10	0.34	161.7	1	0

>TRINITY_DN124992_c1_g1_i2 from sample A1 / Example spliced isoform

CTGGGGAGAGCAAGTTTGTATGTACCTTTTTTGCTGATATCTTAGTTTCTTGGCCTGTTAGCAAAGCAGTGAATTTTCCCAGAAGCTTGTGATGCTAAAGTTTGTAACTTTTGCATCAGGCTATGAACGCCTCTTTGTTTTTCCTCTAGGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCA
GATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTGGTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGG
ACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

>TRINITY_DN124992_c1_g1_i5 from sample A1 / Example unspliced isoform:

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTATTAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTTTTTGTGT
GGGCTGCTCTTCAGGACACGACACTCGTCATTTTGATGATTTGTGCGGTCTTGTCACTTGCTTTGGGCATAAAAACGGAAGGCATTAAGGAAGGGTGGTTAGATGGTGCAAGTATATCTTTTGCTGTGATCCTTGTCATTCTTGTTACAGCAATCAGTGATTACAGACAGTCTCTCCAGTTCCAAAATCTCAATGAAGAG
AAGGAAAACATTCAACTTCAGGTGATCAGAGGTGGAAGAATATTTAAAATTTCTATATATGATCTTGTTGTTGGTGATGTTGTCCCCCTCAATATTGGTGATCAGGTGCCAGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGACTGGGGAGAGCAAGTTTGCTATGAACGCCTCTTT
GTTTTTCCTCTAGGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCAGATGGCTATGGTACAATGTTGgtgagttgttatgcctggttatttgtctggaaataatatcgccacttatgatttgagcagccccgttgacctacttttggttatttgttggacatcaacagacttatcttatctt
tctctatcattggtgttttttgccatgtttgataatagcgtggggtgggttggatttttattacctttacagGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCTGTCTTCCCTGCCTTGTTTAATTTGTATGCTATTCTTTGAC
TTGAGTGGTAGCTTCAGAATCTCAGATATTTCAGTCACAAAGACTCTCCCGTTTATAATAGGTCCGCCTCAATGGTGTTGCAACTTTAATTGGTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGGACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCA
AGACCAAGATTGGAGATGCC

Intron 8 GTAAGCTGAG ... ATGCTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.16	54	6	41
A2	27.27	24	3	9
A3	34.62	34	3	18
A4	40.54	22	2	15
A5	38.24	21	0	13
A6	50	1	0	1
A7	10	18	0	2
A8	22.22	14	0	4
A9	37.5	5	0	3
A10	26.32	14	0	5

>TRINITY_DN124992_c1_g1_i6 from sample A1 / Example spliced isoform

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTATTAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTTTTTGTGT
GGGCTGCTCTTCAGGACACGACACTCGTCATTTTGATGATTTGTGCGGTCTTGTCACTTGCTTTGGGCATAAAAACGGAAGGCATTAAGGAAGGGTGGTTAGATGGTGCAAGTATATCTTTTGCTGTGATCCTTGTCATTCTTGTTACAGCAATCAGTGATTACAGACAGTCTCTCCAGTTCCAAAATCTCAATGAAGAG
AAGGAAAACATTCAACTTCAGGTGATCAGAGGTGGAAGAATATTTAAAATTTCTATATATGATCTTGTTGTTGGTGATGTTGTCCCCCTCAATATTGGTGATCAGGTGCCAGCAGATGGCATTCTTATTACTGGACATTCCCTGTCAATTGATGAATCTAGTATGACTGGGGAGAGCAAGTTTGCTATGAACGCCTCTTT
GTTTTTCCTCTAGGTTTATAAAGATCAGAAAAATCCCTTCATGATGTCTGGCTGTAAGGTGGCAGATGGCTATGGTACAATGTTGGTAACTGGTGTGGGATTAAATACTGAGTGGGGTTTAGTGATGGCTAGAATATCAAAGGATAATGGGGAAGAAACTCCCCTACAGGTCCGCCTCAATGGTGTTGCAACTTTAATTG
GTAAAGTTGGACTTGCAGTGGCAGTTGTAACCTTCGTTATCCTTTTGATTAGATTCTTTACTGGACATACGGAAGACAAAAATGGGAAACCCATGTTTCAAGCAGGCAAGACCAAGATTGGAGATGCC

>TRINITY_DN124992_c1_g1_i4 from sample A1 / Example unspliced isoform:

AACAGTTGCAAACTTCGTCAGGGAACATGATATTAATGCTCTACAGCAGATGGGCGGGgtaagctgagaaaaagattgggattttgattttgttcatacatttgaattacctgttgaggtagttattacagtgacaacctgaaatgctaacagGTAAATCAAGTAGCCAATCTATTACATACTGATCTTCAGAAGGGTAT
TAAGGGAGATCCAGAAGAAATTCAATGTCGAAGGAATGCATATGGAGGGAACTCATATCCACGAAAGCCCCCACCCAAATTTTTTGTAATGTGCTCTTTAGCATTCATGATAGATCAAAGTGTATATTTGCTCTACAGAAGTATTTATGATCCTGTCTGAAAATGGCAGTGTAGTAATTTGCATATCTGAACTTATATCC
TTTTTCAGGTTTTTGTGTGGGC