sp|Q94F62|BAK1_ARATH ----> BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1

Intron 1 GTGCGTATAT ... GGGTTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.43	16	4	10
A2	44.68	13	5	16
A3	33.33	16	10	6
A4	42.11	11	7	9
A5	50	8	10	6
A6	20	2	1	0
A7	0	3	0	0
A8	14.29	3	0	1
A9	25	3	0	2
A10	50	1	1	1

>TRINITY_DN111067_c0_g2_i6 from sample A3 / Example spliced isoform

TCCATAGCAATGGTTTATGAATTTATGGTGTCAATTTTTAATAGCAGTTAAAGAATCGGTAATCCGTACTGTATTGTTCATGGGTTGGCAGGCCAAATAATTAATTGTTCATGCGTACCTCGCCCGCTCCTCTCTTTGTGTTCGGCTTCCATGTTGTGAACGCTCCGGCAATTCTCTGTCATTTCGGAACCGAGACAATG
TGGCAGCATGGAGTTCTATTGGCTAACCTGATTTGCATATGCCTTAATTCTTTCTTGGCCAAATAATTAATTGTTCATGCGCAGCTCAATACTGTATTGTACATGCATTGCCAGATCAGGAAATTCTGTGTTCGGCTTCCATGTTGTGAACGCTATGGCAATTCTGTCAGTTCGGTAGATTAGATCAGAAAAGTATTTCC
ATCCAGATTAGATGGAGAGTTTGTAGGCATCTAAGTTTCTGCAGGGAGC

>TRINITY_DN111067_c0_g2_i7 from sample A3 / Example unspliced isoform:

TCCATAGCAATGGTTTATGAATTTATGGTGTCAATTTTTAATAGCAGTTAAAGAATCGGTAATCCGTACTGTATTGTTCATGGGTTGGCAGGCCAAATAATTAATTGTTCATGCGTACCTCGCCCGCTCCTCTCTTTGTGTTCGGCTTCCATGTTGTGAACGCTCCGGCAATTCTCTGTCATTTCGGAACCGAGACAATG
TGGCAGCATGGAGTTCTATTGGCTAACCTGATTTGCATATGCCTTAATTCTTTCTTGgtgcgtatatatctttattgatattttgttagatcagattattattttgatgtattttaaaagaattcatccatagaaattgtttatgaatttatggtgtcaatttttaatagcagttaaggaatcggtaatcggtactgttt
tgttcatgggttggcagGCCAAATAATTAATTGTTCATGCGCAGCTCAATACTGTATTGTACATGCATTGCCAGATCAGGAAATTCTGTGTTCGGCTTCCATGTTGTGAACGCTATGGCAATTCTGTCAGTTCGGTAGATTAGATCAGAAAAGTATTTCCATCCAGATTAGATGGAGAGTTTGTAGGCATCTAAGTTTCT
GCAGGGAGCGGGGAATGGAACCCGTCAAGTTTCAGTTTGACATTGTAACATTTAAGCGTAGGGAAAGATGACTTACTGATTTTCCCCATGAAGCCGGCATTGCCGAGAGATCGACTCTGTTGAGAGAAAGATTTGTCAACATAGAAGCTGAGGAAGTGAGATCGAGTCCTTTCAAGTTAGGCAGATTTTCAAGCTTCCAT
GGTACGGTACCTGTAAATCTAGCTGTGTACAGGTTTAGTAGTTTCAAGTTGTTCAAAAAACCCATCTCTATAGGAATAGGAAGGCCAGTAAAATTATTGAAGCTAACATCCAAACGCTGCAAAGCC

Intron 2 GTATGGGTAT ... TAATGTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.78	3	5	16
A2	27.78	13	3	7
A3	40.35	17	10	13
A4	20	12	5	1
A5	50	1	0	2
A6	0	2	0	0
A7	5.26	9	0	1
A8	11.11	4	0	1
A9	9.09	5	0	1
A10	14.29	3	1	0

>TRINITY_DN111067_c0_g2_i2 from sample A3 / Example spliced isoform

TCCATAGCAATGGTTTATGAATTTATGGTGTCAATTTTTAATAGCAGTTAAAGAATCGGTAATCCGTACTGTATTGTTCATGGGTTGGCAGGCCAAATAATTAATTGTTCATGCGTACCTCGCCCGCTCCTCTCTTTGTGTTCGGCTTCCATGTTGTGAACGCTCCGGCAATTCTCTGTCATTTCGGAACCGAGACAATG
TGGCAGCATGGAGTTCTATTGGCTAACCTGATTTGCATATGCCTTAATTCTTTCTTGCCAAATAATTAATTGTTCATGCGCAGCTCAATACTGTATTGTACATGCATTGCCAGATCAGGAAATTCTGTGTTCGGCTTCCATGTTGTGAACGCTATGGCAATTCTGTCATTTCGGAACCGAGACAATGTGGGAGCATGGAG
TTCGGTAGATTAGATCAGAAAAGTATTTCCATCCAGATTAGATGGAGAGTTTGTAGGCATCTAAGTTTCTGCAGGGAGCGGGGAATGGAACCCGTCAAGTTTCAGTTTGACATTGTAACATTTAAGCGTAGGGAAAGATGACTTACTGATTTTCCCCATGAAGCCGGCATTGCCGAGAGATCGACTCTGTTGAGAGAAAG
ATTTGTCAACATAGAAGCTGAGGAAGTGAGATCGAGTCCTTTCAAGTTAGGCAGATTTTCAAGCTTCCATGATGTTTGTGGAGCATACACCGATTTTAGTTATGTTACGGCCGGCGGATTAAAGATGTAGCATGGATTTCTATTTGCTAACCTGATCGGATCTTGGAAGAAAATCGAAAATAGTTGGATCCTCTATATAC
CCGAATGCAAAAGGAAACGAAACAGGCCCCCTGCATCAACGATTCCGAGCAGGCAATCCGATACGCAATTGACCATTTTCTACCGTGGAACGGTGAAATATTCCCGCAGATAAGAACGAATGTCGATGGCAGACAGAGCTGAAAACATCTCTCCCTGTCATGAGTTATCGGAGGGATCGAGGATCCACCACCAACCTATT
AATTGCAAGGTGCACACAGGTACGATTAGGGCAACCAATTCTCGGAGACCATCCATAGATTAATCATTACATTATCTACAAATTCCTTCTTAGTTTTTCAAATTATGCCTCGAAATTTAATTAATGTTTTCCTGCTTTGATACTGTTACTGTTAAAGTTGTACGATTAAGAGATTTGAACTAACATCTACTATTATATTA
AAATTAACCATATAAAATATACTGAAATACTCG

>TRINITY_DN111067_c0_g2_i10 from sample A3 / Example unspliced isoform:

TCGGGATGGTTGTTCTGGGTTTGTAACGAGTTCTAGGTGTGGGATTGTAATGTGTAGAATAAGTACTTGGGCTTTTATTTTCAACTAATTCTTTGGTTTTTTAATCCAGGCCCCCTGCATCAACGATTCCGAGCAGGCAATCCGATACGCAATTGACCATTTTCTACCGTGGAACGGTGAAATATTCCCGCAGATAAGgt
atgggtatggtaattgaaatagttatgggatagacaaggactttgaaagattggcaggatttagtttagattagaatataatggcgtctgtctcttaatgtccagAACGAATGTCGATGGCAGACAGAGCTGAAAACATCTCTCCCTGTCATGAGTTATCGGAGGGATCGAGGATCCACCACCAACCTATTAATTGCAAG
GTGCACACAGGTACGATTAGGGCAACCAATTCTCGGAGACCATCCATAGATTAATCATTACATTATCTACAAATTCCTTCTTAGTTTTTCAAATTATGCCTCGAAATTTAATTAATGTTTTCCTGCTTTGATACTGTTACTGTTAAAGTTGTACGATTAAGAGATTTGAACTAACATCTACTATTATATTAAAATTAACC
ATATAAAATATACTGAAATACTCG

Intron 3 GCCAAATAAT ... TCATGCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.6	12	18	24
A2	77.71	4	14	14
A3	41.27	18	12.5	13
A4	52.5	11	11	15
A5	76.39	1	3	3.5
A6	55	2	2.5	2.5
A7	76.39	1	3	3.5
A8	35.09	6	3	3.5
A9	61.82	2	3	3.5
A10	0	1	0	0

>TRINITY_DN121127_c5_g3_i9 from sample A1 / Example spliced isoform

GAACGCTCCGGCAATTCTCTGTCATTTCGGAACCGAGACAATGTGGCAGCATGGAGTTCTATTGGCTAACCTGATCTGCATATGCCTTAATTCTTTCTTGCTCAATACTGTATTGTACATGCATTGCCAGATCAGGAAATTCTGTGTTCGGCTTCCATGTTGTGAACGCTATGGCAATTCTGTCATTTCGGAACCGAGAC
AATGTGGCAGCATGG

>TRINITY_DN121127_c5_g3_i11 from sample A1 / Example unspliced isoform:

GAACGCTCCGGCAATTCTCTGTCATTTCGGAACCGAGACAATGTGGCAGCATGGAGTTCTATTGGCTAACCTGATCTGCATATGCCTTAATTCTTTCTTGgccaaataattaattgttcatgcgcagCTCAATACTGTATTGTACATGCATTGCCAGATCAGGAAATTCTGTGTTCGGCTTCCATGTTGTGAACGCTATG
GCAATTCTGTCAGTTCGGTAGATTAGATCAGAAAAGTATTTCCATCCAGATTAGATGGAGAGTTTGTAGGCATCTAAGTTTCTGCAGGGAGCGGGGAATGGAACCCGTCAAGTTTCAGTTTGACATTGTAACATTTAAGCGTAGGGAAAGATGACTTACTGATTTTCCCCATGAAGCCGGCATTGCCGAGAGATCGACTC
TGTTGAGAGAAAGATTTGTCAACATAGAAGCTGAGGAAGTGAGATCGAGTCCTTTCAAGTTAGGCAGATTTTCAAGCTTCCATGGTACGGTACCTGTAAATCTAGCTGTGTACAGGTTTAGTAGTTTCAAGTTGTTCAAAATAGAATGTTGGAATTTGCAAACACCCTATAAATTAGATCAGATTATTCTTTCTTGGTGA
GTCTACTTCTTTATTGATTAGATCAGATTATTGTTTTGATGTATTTTAAAACGCATTTGAATTTATCCATAGAAATTGTTTATGAATTTATGGTGTCAATTTTTAATAGCAGTTAAGGAATCGGTAATCGGTACTGTTTTGTTCATGGGTTGGCAGGCCAAATAATTAATTGTTCATG

Intron 4 GTGAGTTTAG ... AGATTTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.83	3	16.3	9
A2	15.81	10.7	2	2
A3	38.05	11.7	8.3	6
A4	44.44	10	10	6
A5	100	0	3	4
A6	25	6	0	4
A7	28.33	4.7	1.7	2
A8	24.44	1.7	0	1
A9	0	12.3	0	0
A10	12.76	10.3	0	3

>TRINITY_DN111067_c0_g2_i2 from sample A3 / Example spliced isoform

TCCATAGCAATGGTTTATGAATTTATGGTGTCAATTTTTAATAGCAGTTAAAGAATCGGTAATCCGTACTGTATTGTTCATGGGTTGGCAGGCCAAATAATTAATTGTTCATGCGTACCTCGCCCGCTCCTCTCTTTGTGTTCGGCTTCCATGTTGTGAACGCTCCGGCAATTCTCTGTCATTTCGGAACCGAGACAATG
TGGCAGCATGGAGTTCTATTGGCTAACCTGATTTGCATATGCCTTAATTCTTTCTTGCCAAATAATTAATTGTTCATGCGCAGCTCAATACTGTATTGTACATGCATTGCCAGATCAGGAAATTCTGTGTTCGGCTTCCATGTTGTGAACGCTATGGCAATTCTGTCATTTCGGAACCGAGACAATGTGGGAGCATGGAG
TTCGGTAGATTAGATCAGAAAAGTATTTCCATCCAGATTAGATGGAGAGTTTGTAGGCATCTAAGTTTCTGCAGGGAGCGGGGAATGGAACCCGTCAAGTTTCAGTTTGACATTGTAACATTTAAGCGTAGGGAAAGATGACTTACTGATTTTCCCCATGAAGCCGGCATTGCCGAGAGATCGACTCTGTTGAGAGAAAG
ATTTGTCAACATAGAAGCTGAGGAAGTGAGATCGAGTCCTTTCAAGTTAGGCAGATTTTCAAGCTTCCATGATGTTTGTGGAGCATACACCGATTTTAGTTATGTTACGGCCGGCGGATTAAAGATGTAGCATGGATTTCTATTTGCTAACCTGATCGGATCTTGGAAGAAAATCGAAAATAGTTGGATCCTCTATATAC
CCGAATGCAAAAGGAAACGAAACAGGCCCCCTGCATCAACGATTCCGAGCAGGCAATCCGATACGCAATTGACCATTTTCTACCGTGGAACGGTGAAATATTCCCGCAGATAAGAACGAATGTCGATGGCAGACAGAGCTGAAAACATCTCTCCCTGTCATGAGTTATCGGAGGGATCGAGGATCCACCACCAACCTATT
AATTGCAAGGTGCACACAGGTACGATTAGGGCAACCAATTCTCGGAGACCATCCATAGATTAATCATTACATTATCTACAAATTCCTTCTTAGTTTTTCAAATTATGCCTCGAAATTTAATTAATGTTTTCCTGCTTTGATACTGTTACTGTTAAAGTTGTACGATTAAGAGATTTGAACTAACATCTACTATTATATTA
AAATTAACCATATAAAATATACTGAAATACTCG

>TRINITY_DN111067_c0_g2_i9 from sample A3 / Example unspliced isoform:

CGGCAATTCTCTGTCATTTCGGAACCGAGACAATGTGGCAGCATGGAGTTCTATTGGCTAACCTGATTTGCATATGCCCTAATTCTTTTTTGGTGACTATACTTCTTTATTGATATTTTGTTAGATCAGATTATTATTTTGATGTATTTTAAAAGAATTCATCCATAGAAATTGTTTATGAATTTATGCTGTCAATTTTT
AATAGCAGTTAAAGAATCGGTAATCGGTACTGTATTGTTCATGCATTGGTAGGCCAAATGGCAGCATCACGTGCAGGCCAATTAACGAAGTAGTAGAGACAGAGATTTATGAGCAAGAAAATCGAAAAATAGCCGGATCGTCTACAGTTTCAGTTTCATTCTCACAGCTTCGCTTTTCTAAAATAACAAGAACTTCATCC
ATGTTGACGAGTAAACTAGATTTTCCATGAATTTCTTTTCTATCTGACAGTTCAGATTCTAGGTTCTAGGGAATGCTCGGGTTAGGTTTAATTTAGATCTTCCCATGTATTTCGAAGTGAAGGATTTTAGATCTAGTTAGTTAGACATTACATGAAATTTGACCATTTCTTAGAAAAATGTCTCGGTATTGTATTATATA
ATTAAGAGAAAAGAGTTGACCATTTGTTAACTTTTATGATATGACAAGACTCCATCAGTGAAATTCTTGCAGATGTTTGTGGAGCATACACCGATTTTAGTTATGTTACGGCCGGCGGATTAAAGATGTAGCATGGATTTCTATTTGCTAACCTGATCGGATCTTGgtgagtttagtactttatttggtattttgttgga
tcagtagatcggattattattttaacctaatcggattatgacgattttagtattttattggtattttgttggatcagaagatcggattattattttatttgatgcattctgtttatgaatgcattggtattttgttagactagaagatcagatttctagtttatgaattcatcaggttaatttttaatcacaattaagta
acaaatactgtatggttcatggcttgactgagatttttagGAAGAAAATCGAAAATAGTTGGATCCTCTATATACCCGAATGCAAAAGGAAACGAAACAGGCCCCCTGCATCAACGATTCCGAGCAGGCAATCCGATACGCAATTGACCATTTTCTACCGTGGAACGGTGAAATATTCCCGCAGATAAGAACGAATGTCG
ATGGCAGACAGAGCTGAAAACATCTCTCCCTGTCATGAGTTATCGGAGGGATCGAGGATCCACCACCAACCTATTAATTGCAAGGTGCACACAGGTACGATTAGGGCAACCAATTCTCGGAGACCATCCATAGATTAATCATTACATTATCTACAAATTCCTTCTTAGTTTTTCAAATTATGCCTCGAAATTTAATTAAT
GTTTTCCTGCTTTGATACTGTTACTGTTAAAGTTGTACGATTAAGAGATTTGAACTAACATCTACTATTATATTAAAATTAACCATATAAAATATACTGAAATACTCG